PI0025390 (gene) Melon (PI 482460) v1

Overview
NamePI0025390
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionL-galactono-1,4-lactone dehydrogenase
Locationchr08: 4945266 .. 4950258 (-)
RNA-Seq ExpressionPI0025390
SyntenyPI0025390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTCAATTGAATTCCTTTGTTGGGTTGGGGAATCCATAAACCCAAGAATCCATCTTTCAACTTTCTCAAATGCTCAACTTTCTCTCTCTCCGGCGATCCCTCCATTACTTCCGCCACCGCCGTCTCCAAATCTCACCCACCGCCGCCATTTCCAAACCCCCTTTCAATCCCCTTCGCCCATTTTCCACTGTTTCTCCTTCACCTTCCTCCTCCTCTGATTCCGAGCTTCGTAAGTACCTCGCCTATGGTGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTTCACGAAAACGCCAAGCACAAGAAGGCCCAGATCTTCCGATACGCTCCTCTGCCTGAGGATCTTCACACTGTGTCCAATTGGAGTGGAACTCATGAGGTTTGGACCAGAGTGTTTCACCAGCCGGAGGATTTGGAGCAGCTTGAGCAAGTGGTGAAAGAGGCTAATGAGAAGAAGGCTAGGATTCGGCCTGTTGGCTCCGGGCTTTCGCCTAATGGGATTGGATTATCGAGGATGGGGATGGTGAATTTGGCGTTGATGGATAAAGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTGGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAGATTGGTGGCATCATTCAGGTGGTTATATGAACATTTTTGTGTTTTTCATGTTGTTTAATTGCTAGTAGAATTTTAATATATTTGAAAACTTGAGTATCAATGTCATGGCTGCGTGTTGATAAATATGAACAATCACCTGTCCAGGGTGTACATTAGTGTTTTGAGTGAAGAAAAAGAATACTATTGATTTATTGTCTTAAATAGATTGAAAGTGATTAGATTTGATATGCAAACTTCCATTCGATCTTGATTGGCTTGTATTTGGTATCTTCTAGTGCCAAATTGTCAAGGATGTGATTTTTAAAGGCAGGTCGAGGTTAGTAATAGTGTTAGGAGGTTACATTTGAAACAAAATTTATCTCGTTTGAAGGAGAAATGATCACATTGTTACTCCTAATGATTTGAAATGAACATTGATTGCATAGATGGCTATTTCTTTCAGATTTTACTTTGTTTGATTGGAGTCCCTTGTTGTTTTAGTTTGGCTTTTTTGGTTGGTTGGGGCTTTATTTTGTATGTCCCTCATACTCTTTTGTTTGTTCTTTATAAACAAAAAAGTAATGACTATTTTAGGTGGCATAACTATTATATGTGGGATTCGATTGAAGTTTTCAATTTTTATCTTTTCTCTTGGCTTTTTTGCTTATGGACACTTTTCACAATTATGCATCTAGGTTGGTGCACATGGTACCGGGGCAAAATTGCCTCCAATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCTGCCAAGGGTACCATAGAGATTTCCAAAGATAAAGATCCAGAGCTTTTTTTACTAGCTCGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCTGAAGTAACCCTCCAATGTGTTGAGAGACAGGAACTCGTGGAGCACACATATATTTCGAACATGAAAGACATCAAGAAGAACCACAAGTAATTCCGTACTTTTTGATAGTTAATTTTATACAGTTCTATAGTATGTTAGAGACTTCAGTTTACATCTCATTTTATTTATTCTTATTATTATTTGACAATGTTTAGAGGTTAATTGAAATAGAGCTCTGAATAGGTATATGCTGCCATCTTTGATGCTTTTATGAAACAAGCATTCCATATCTTAGTGAGAGAATTACCAATTTGTGAAAGTTGAATTTTGCCATTTCTTAACCAAAAATATATTTTGAACATAATTAATGGATATAAATGTAATTTTCTAATCTATAAAAGGAAACTTTCTATAGCTGGAATTTTGGGCTCTTCAAGAATGTATCGTGTTTGGAATTCTAATTGATGGAAAGTGAGATTGTGATTCAGATGTTTTTCCATTTTTTTAAACTTTCATACGTAAATGTAGAGCCTTTCTTTTTTCCTCCTCTCTGAAAAACCAAGACCGCAAAACTCCTCTAGAAAAATGGTTTTCCATCCAAGTAAGATTAGAGAAAAATTATAAAAAGTCTTTGAGACCGAAGCTCAATGAGAAACATGGAACCTCACCAAGGTCTGAAGCCCTAGACTTGCAAGCTATACTATTTATTGTGTTATAGTATACAAAAGTTGTATATAGATTGAAATCAAAACTATTGATTCATGGGAGTTGTTGCCTAATGTAGACTTATACTATTAATTTCAATTTGTCTAGTTTTGAACAAGCCTTCTCTTTTTTTTCTAAACTAAATTTAATCCTTTATCTCTACCGTTCACGTCACTCACAAATAGGATGAAACTTTTTTATTTCATTTCATTTCATTTTTTTTGTCTTCATCCTCTAAAGTCAAGTCACTTAAGTAATGTTGAAGAATTACACATTTAAGGTCTGATATTCTCTCTATGCAGGAAATTATTGGCTGACAATAAGCATGTGAAATACCTGTATATTCCTTACACCGATGCTATTGTTGTTGTAACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCCAAATTCAAACCGAAATATACAAGTGAGGAAGCCATACAGCATGTTCGTGATCTCTATGTGGAATCCCTTAAAAAATACAGGTTACTTCCATCTTCTGTTTGAGTATATTTAGTCATCAATAATTTTAGGACGCTGGAAATGGGTTGAAACATAGTTGACATTTTTATCCTCTCAAATCACTTTTCATTCTTTTGATTTCTTTCTCCTTCTCTTTTGGTTGATATTCATTTTTCATCAAAGAGTCACTTGAGGCCTTTTTTCAAATTTATTTGCTATATATACTTTTCTTCCCCATTGTTTTCCCAGTGCTTTTTATTTCATCTAATTGCTTGCTTTATAATTGCAGTGCTAGTGAGAAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGCGATAAACTACTTGCCCTTGATCCTCTTAACAAAGAGCATGTCATGAAAGTGAATCAAGCAGAGGCTGAGTTCTGGAGGAAGTCAGAAGGATACAGAGTCGGATGGAGTGATGAAATCTTGGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAGACTTGCTTTCCAGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATGGAGGAATTGAAGCAACTTATTGAAAAAGAAAACATACCTGCACCTGCTCCTCTTGAGCAGCGGTGGACTGCTCGTAGCAAGAGTCCCATGAGTCCAGCTTCAAGCACAGCTGAGGATGATATATTCTCTTGGGTAAACTCTAAATTTCAAACTTTTTACTCTCTCTTTCAAGTTTTAAATCTATACTTGTGCAGCCCTGAGGGTTGCCCTGGTGATAAAGACAGGGACGTTTAGAGGTATCCTCCAAGATTTTGGAACTTAATTACTTAAAACAATCCTTGTTCTTTTGGAGTTGGTATTGGGCCAGGCGAGCTTCATAACCACGTGTATAATGGAAAGAGTGTGTCCCCACATCTTGGTTAAAAATTGACTTTCTACACTTGTGCTATTGTGCATGAAGGGAGAAGCACAAGTTATATTTTAAAGAAATAGTGAAACTAAATATCAAGTATTATATAGTTTCTTTCTGCTAAATGGGTCTTATATTCTGAAGAGTGTTGTGATCTTATATGATGATTAGTTGCTGATGAGTGAGTACTCAAATTCATTTTCTTTCAGTAGCTTCTGGGCTTTACCATCTCAAAAGCACTAACACTTTAGTTTGGCCAATGTGTCTGTATCCAACCTGTGTCAATCACTTGTTGCACATGTATCAAACACTTGTTAGTGCAATACATATGTTAAACATTAGTTATACAAAGTCCAAATAAGGACTGACATTTGTTAGACATGCATCAAATATTTGTTTCATATACTAAAACTAAAGACACATAAATGACAAAAAGAAAAAAAAATTGAGAGCAAAATACTGAATTTTTAAGCATATAAATGCCTAACTTATTGACTTTGATGTACTTGTATAAAATTTTATGTATTTTAAAGAAGTACGTTTTAACCTTTAGTACATGTGTTCTTGTTGTGTTTGTGTCCTAAATTACAAAAATGATGTGTCGCTGTGTTCTTGTTGCATGAGTGTGGTGTTGTGCCACATCCGTATCTGTGCTTCTCTCGACAGAAATCTTTACTCAATTGCCAACAGATTTTATTTTGATGTCTCACCAATTAGAACTCAGTAAACCCAACTGTGGTTTCTTCTTTGTTAATCTTGTTAATTTGATTGATTGTCTTTTACTGCGATTAGGTCGGAATAATCATGTACCTACCTACGTCAGATGCGCGCCAGAGAAAGGAAATCACAGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTATGGGATCAATATTCTGCCTTTGAACATTGGGCTAAGATCGAGGTACCCAATTTCAAAATCTTTTCCTGATTAAGCCCTACAAAAATGGGGGCATAACGTTTTGACCACTTTGTTGTATTGTATGTAAAATCCAGGTTCCCAAGGACAAGGATGAGCTTGCAGCCCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTTGACGAGTACAACAAGGCACGACAGGCATTGGACCCGAACAAAATTCTTTCAAATAACAAGTTGGAAAAACTGTTCTCGTCATCGGATACGGTTTGAGATGCACATATGTTTTAGTGATCAATAATAAGTCCTGTTATCCAGTTGGATAAGTGTTGTTTTCTGGCTGTACTGTACATGTTCACAGGAATGGTGTATTTGTCAATTCCTTTGGGTATATTATTTCTTTGAATTCTTTTGCCATAAAAAGTATTCTTGAGGAAGGAGATTATTTTCCATGGCCTGTTCACATTTTAAAATGATAATACCAAATTTTGGATGTTTTTGGGA

mRNA sequence

GCTCAATTGAATTCCTTTGTTGGGTTGGGGAATCCATAAACCCAAGAATCCATCTTTCAACTTTCTCAAATGCTCAACTTTCTCTCTCTCCGGCGATCCCTCCATTACTTCCGCCACCGCCGTCTCCAAATCTCACCCACCGCCGCCATTTCCAAACCCCCTTTCAATCCCCTTCGCCCATTTTCCACTGTTTCTCCTTCACCTTCCTCCTCCTCTGATTCCGAGCTTCGTAAGTACCTCGCCTATGGTGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTTCACGAAAACGCCAAGCACAAGAAGGCCCAGATCTTCCGATACGCTCCTCTGCCTGAGGATCTTCACACTGTGTCCAATTGGAGTGGAACTCATGAGGTTTGGACCAGAGTGTTTCACCAGCCGGAGGATTTGGAGCAGCTTGAGCAAGTGGTGAAAGAGGCTAATGAGAAGAAGGCTAGGATTCGGCCTGTTGGCTCCGGGCTTTCGCCTAATGGGATTGGATTATCGAGGATGGGGATGGTGAATTTGGCGTTGATGGATAAAGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTGGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAGATTGGTGGCATCATTCAGGTTGGTGCACATGGTACCGGGGCAAAATTGCCTCCAATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCTGCCAAGGGTACCATAGAGATTTCCAAAGATAAAGATCCAGAGCTTTTTTTACTAGCTCGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCTGAAGTAACCCTCCAATGTGTTGAGAGACAGGAACTCGTGGAGCACACATATATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAATTATTGGCTGACAATAAGCATGTGAAATACCTGTATATTCCTTACACCGATGCTATTGTTGTTGTAACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCCAAATTCAAACCGAAATATACAAGTGAGGAAGCCATACAGCATGTTCGTGATCTCTATGTGGAATCCCTTAAAAAATACAGTGCTAGTGAGAAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGCGATAAACTACTTGCCCTTGATCCTCTTAACAAAGAGCATGTCATGAAAGTGAATCAAGCAGAGGCTGAGTTCTGGAGGAAGTCAGAAGGATACAGAGTCGGATGGAGTGATGAAATCTTGGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAGACTTGCTTTCCAGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATGGAGGAATTGAAGCAACTTATTGAAAAAGAAAACATACCTGCACCTGCTCCTCTTGAGCAGCGGTGGACTGCTCGTAGCAAGAGTCCCATGAGTCCAGCTTCAAGCACAGCTGAGGATGATATATTCTCTTGGGTCGGAATAATCATGTACCTACCTACGTCAGATGCGCGCCAGAGAAAGGAAATCACAGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTATGGGATCAATATTCTGCCTTTGAACATTGGGCTAAGATCGAGGTTCCCAAGGACAAGGATGAGCTTGCAGCCCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTTGACGAGTACAACAAGGCACGACAGGCATTGGACCCGAACAAAATTCTTTCAAATAACAAGTTGGAAAAACTGTTCTCGTCATCGGATACGGTTTGAGATGCACATATGTTTTAGTGATCAATAATAAGTCCTGTTATCCAGTTGGATAAGTGTTGTTTTCTGGCTGTACTGTACATGTTCACAGGAATGGTGTATTTGTCAATTCCTTTGGGTATATTATTTCTTTGAATTCTTTTGCCATAAAAAGTATTCTTGAGGAAGGAGATTATTTTCCATGGCCTGTTCACATTTTAAAATGATAATACCAAATTTTGGATGTTTTTGGGA

Coding sequence (CDS)

ATGCTCAACTTTCTCTCTCTCCGGCGATCCCTCCATTACTTCCGCCACCGCCGTCTCCAAATCTCACCCACCGCCGCCATTTCCAAACCCCCTTTCAATCCCCTTCGCCCATTTTCCACTGTTTCTCCTTCACCTTCCTCCTCCTCTGATTCCGAGCTTCGTAAGTACCTCGCCTATGGTGCTCTCGTCATTTTCTGCGGCGCCGCCACTTACTACTCCTTCCCTTTTCACGAAAACGCCAAGCACAAGAAGGCCCAGATCTTCCGATACGCTCCTCTGCCTGAGGATCTTCACACTGTGTCCAATTGGAGTGGAACTCATGAGGTTTGGACCAGAGTGTTTCACCAGCCGGAGGATTTGGAGCAGCTTGAGCAAGTGGTGAAAGAGGCTAATGAGAAGAAGGCTAGGATTCGGCCTGTTGGCTCCGGGCTTTCGCCTAATGGGATTGGATTATCGAGGATGGGGATGGTGAATTTGGCGTTGATGGATAAAGTTTTGGAAGTAGATAAGGAGAAGAAGAGGGTTAGGGTTCAAGCGGGAATAAGGGTTCAGCAGCTTGTGGATGGAATCAAGGAGTATGGGCTTACTTTGCAGAATTTTGCTTCTATCAGGGAGCAGCAGATTGGTGGCATCATTCAGGTTGGTGCACATGGTACCGGGGCAAAATTGCCTCCAATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCTGCCAAGGGTACCATAGAGATTTCCAAAGATAAAGATCCAGAGCTTTTTTTACTAGCTCGCTGTGGTCTTGGGGGGCTTGGAGTGGTTGCTGAAGTAACCCTCCAATGTGTTGAGAGACAGGAACTCGTGGAGCACACATATATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAATTATTGGCTGACAATAAGCATGTGAAATACCTGTATATTCCTTACACCGATGCTATTGTTGTTGTAACTTGCAATCCTATCTCAAAATGGAGAGGCCCTCCCAAATTCAAACCGAAATATACAAGTGAGGAAGCCATACAGCATGTTCGTGATCTCTATGTGGAATCCCTTAAAAAATACAGTGCTAGTGAGAAAAGAGACATAAATGAATTTTCATTTACTGAGTTGCGCGATAAACTACTTGCCCTTGATCCTCTTAACAAAGAGCATGTCATGAAAGTGAATCAAGCAGAGGCTGAGTTCTGGAGGAAGTCAGAAGGATACAGAGTCGGATGGAGTGATGAAATCTTGGGTTTTGATTGTGGTGGCCAACAATGGGTCTCTGAGACTTGCTTTCCAGCCGGTACTCTTGCAAAACCTAACATGAAAGACATTGAATACATGGAGGAATTGAAGCAACTTATTGAAAAAGAAAACATACCTGCACCTGCTCCTCTTGAGCAGCGGTGGACTGCTCGTAGCAAGAGTCCCATGAGTCCAGCTTCAAGCACAGCTGAGGATGATATATTCTCTTGGGTCGGAATAATCATGTACCTACCTACGTCAGATGCGCGCCAGAGAAAGGAAATCACAGAGGAGTTCTTTCACTATAGGCACCTCACCCAAACACTGCTATGGGATCAATATTCTGCCTTTGAACATTGGGCTAAGATCGAGGTTCCCAAGGACAAGGATGAGCTTGCAGCCCTGCAAGCAAGACTTAGGAAACGGTTTCCTGTTGACGAGTACAACAAGGCACGACAGGCATTGGACCCGAACAAAATTCTTTCAAATAACAAGTTGGAAAAACTGTTCTCGTCATCGGATACGGTTTGA

Protein sequence

MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVSPSPSSSSDSELRKYLAYGALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEDLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV
Homology
[Close]
BLAST of PI0025390 vs. ExPASy Swiss-Prot
Match: O47881 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=3712 PE=1 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 2.3e-267
Identity = 467/612 (76.31%), Postives = 514/612 (83.99%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPF----STVSPSP----------- 60
           ML  L LRRS              A   +PPF PLR       T++P+P           
Sbjct: 1   MLRSLLLRRS-------------NARSLRPPFPPLRTLCTSGQTLTPAPPPPPPPPPPIS 60

Query: 61  SSSSDSELRKYLAYGALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSG 120
           SS+S+ E RKY  Y AL +F GAATY+SFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSG
Sbjct: 61  SSASEKEFRKYAGYAALALFSGAATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVWTRVFHQPEDLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKV 180
           THEV TR F+QPE L  LE +VKEA+EKK RIRPVGSGLSPNGIGLSR GMVNLALMDKV
Sbjct: 121 THEVQTRNFNQPETLADLEALVKEAHEKKNRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPP 240
           LEVDKEKKRVRVQAGIRVQQLVD I+EYGLTLQNFASIREQQIGGIIQVGAHGTGA+LPP
Sbjct: 181 LEVDKEKKRVRVQAGIRVQQLVDAIQEYGLTLQNFASIREQQIGGIIQVGAHGTGARLPP 240

Query: 241 IDEQVIAMKLVTPAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTY 300
           IDEQVI MKLVTPAKGTIE+SKD DPELF LARCGLGGLGVVAEVTLQCVERQEL+EHTY
Sbjct: 241 IDEQVIGMKLVTPAKGTIELSKDNDPELFHLARCGLGGLGVVAEVTLQCVERQELLEHTY 300

Query: 301 ISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQ 360
           +S +++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW G PK KPKYT+EEA++
Sbjct: 301 VSTLEEIKKNHKKLLSTNKHVKYLYIPYTDTVVVVTCNPVSKWSGAPKDKPKYTTEEALK 360

Query: 361 HVRDLYVESLKKYSA---------SEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAE 420
           HVRDLY ES+ KY           S + DINE SFTELRDKL+ALDPLN  HV KVNQAE
Sbjct: 361 HVRDLYRESIVKYRVQDSSKKTPDSREPDINELSFTELRDKLIALDPLNDVHVGKVNQAE 420

Query: 421 AEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKE 480
           AEFW+KSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKP+MKD+EY+E+LK+LI+KE
Sbjct: 421 AEFWKKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPSMKDLEYIEQLKELIQKE 480

Query: 481 NIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYR 540
            IPAP+P+EQRWT RSKSPMSPA STAE+DIFSWVGIIMYLPT+D RQRK+IT+EFFHYR
Sbjct: 481 AIPAPSPIEQRWTGRSKSPMSPAFSTAEEDIFSWVGIIMYLPTADPRQRKDITDEFFHYR 540

Query: 541 HLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARQALDPNKILSN 589
           HLTQ  LWDQYSA+EHWAKIE+PKDK+EL ALQ RLRKRFPVD YNKAR+ LDPN+ILSN
Sbjct: 541 HLTQAKLWDQYSAYEHWAKIEIPKDKEELEALQERLRKRFPVDAYNKARRELDPNRILSN 599

[Close]
BLAST of PI0025390 vs. ExPASy Swiss-Prot
Match: Q9SU56 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GLDH PE=1 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 1.2e-266
Identity = 461/612 (75.33%), Postives = 518/612 (84.64%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVS---------------PSP 60
           ML  L LRRS+    H    +SP+++  +  F+P R   T                 P P
Sbjct: 1   MLRSLLLRRSV---GHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRPPPPPP 60

Query: 61  SSSSDSELRKYLAYGALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSG 120
           +++S+++ RKY  Y AL IF G ATY+SFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSG
Sbjct: 61  ATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVWTRVFHQPEDLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKV 180
           THEV TR F+QPE+L  LE +VKE++EKK RIRPVGSGLSPNGIGLSR GMVNLALMDKV
Sbjct: 121 THEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPP 240
           LEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQVGAHGTGA+LPP
Sbjct: 181 LEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPP 240

Query: 241 IDEQVIAMKLVTPAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTY 300
           IDEQVI+MKLVTPAKGTIE+S++KDPELF LARCGLGGLGVVAEVTLQCV R ELVEHTY
Sbjct: 241 IDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHTY 300

Query: 301 ISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQ 360
           +SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK KPKYT++EA+Q
Sbjct: 301 VSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAVQ 360

Query: 361 HVRDLYVESLKKYSA---------SEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAE 420
           HVRDLY ES+ KY           S + DI E SFTELRDKLLALDPLN  HV KVNQAE
Sbjct: 361 HVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQAE 420

Query: 421 AEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKE 480
           AEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EY+EELK+LIEKE
Sbjct: 421 AEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEKE 480

Query: 481 NIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYR 540
            IPAPAP+EQRWTARSKSP+SPA ST+EDDIFSWVGIIMYLPT+D RQRK+IT+EFFHYR
Sbjct: 481 AIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTADPRQRKDITDEFFHYR 540

Query: 541 HLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARQALDPNKILSN 589
           HLTQ  LWDQ+SA+EHWAKIE+PKDK+EL ALQAR+RKRFPVD YNKAR+ LDPN+ILSN
Sbjct: 541 HLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDAYNKARRELDPNRILSN 600

[Close]
BLAST of PI0025390 vs. ExPASy Swiss-Prot
Match: Q2RAP0 (L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH1 PE=2 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 4.1e-240
Identity = 412/570 (72.28%), Postives = 481/570 (84.39%), Query Frame = 0 

Query: 17  RRLQISPTAAISKPPFNPLRPFSTVSPSPSSSSDSELRKYLAYGALVIFCGAATYYSFPF 76
           RR   SP+   S    + +R  S  SP P  +SD++LRKY  Y  L++ CGAATYYSFP 
Sbjct: 11  RRASSSPS---SHHHLHLVRALSASSPLP--ASDADLRKYAGYALLLLGCGAATYYSFPL 70

Query: 77  HENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEDLEQLEQVVKEANEKKAR 136
             +A HKKA  F+YAPLP+DLH VSNWS THEV TRV  QP+ L  L   +  A+ +  +
Sbjct: 71  PPDALHKKAVPFKYAPLPDDLHAVSNWSATHEVHTRVLLQPDSLPALHDALAAAHGECRK 130

Query: 137 IRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLT 196
           +RP+GSGLSPNG+ LSR GMVNLALMDKVL VD +KK V VQAGIRV +LVD ++E+GLT
Sbjct: 131 LRPLGSGLSPNGLALSRAGMVNLALMDKVLGVDAKKKTVTVQAGIRVAELVDALREHGLT 190

Query: 197 LQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFLL 256
           LQNFASIREQQ+GGIIQVGAHGTGA+LPPIDEQVI+MKLVTPAKGTIE+S++KDP+LF L
Sbjct: 191 LQNFASIREQQVGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPDLFYL 250

Query: 257 ARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDA 316
           ARCGLGGLGVVAEVTLQCVER +L+EHT++SN  ++KKNHKK L++NKH+KYL+IPYTD 
Sbjct: 251 ARCGLGGLGVVAEVTLQCVERHQLIEHTFVSNADEVKKNHKKWLSENKHIKYLWIPYTDT 310

Query: 317 IVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY---SASEKRDINEFSFTE 376
           +VVV CNP S+WR  PKF  KY  +EAIQHVRDLY ESLKKY   + S   ++++ SFTE
Sbjct: 311 VVVVQCNPPSRWR-TPKFTSKYGKDEAIQHVRDLYHESLKKYRTKAESNDPEVDQLSFTE 370

Query: 377 LRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAG 436
           LRD+LL LDPL+K+HV+++N+AEAE+W+KSEGYR+GWSDEILGFDCGGQQWVSETCFPAG
Sbjct: 371 LRDRLLTLDPLDKDHVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCFPAG 430

Query: 437 TLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGI 496
           TLAKPNMKD++Y+EEL QLIEKE+IPAPAP+EQRWTA S+SPMSPASS+ EDDIFSWVGI
Sbjct: 431 TLAKPNMKDLDYIEELLQLIEKEDIPAPAPIEQRWTACSRSPMSPASSSQEDDIFSWVGI 490

Query: 497 IMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLR 556
           IMYLPTSDARQRKEITEEFF+YR  TQT LWD YSA+EHWAKIEVPKDKDELA LQARLR
Sbjct: 491 IMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELAELQARLR 550

Query: 557 KRFPVDEYNKARQALDPNKILSNNKLEKLF 584
           KRFPVD YNKAR  LDPNK+LSN KLEKLF
Sbjct: 551 KRFPVDAYNKARMELDPNKVLSNAKLEKLF 574

[Close]
BLAST of PI0025390 vs. ExPASy Swiss-Prot
Match: Q2QXY1 (L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH2 PE=2 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 2.1e-239
Identity = 410/570 (71.93%), Postives = 482/570 (84.56%), Query Frame = 0 

Query: 17  RRLQISPTAAISKPPFNPLRPFSTVSPSPSSSSDSELRKYLAYGALVIFCGAATYYSFPF 76
           RR   SP+   S    + +R  S  SP P  +SD++LRKY  Y  L++ CGAATYYSFP 
Sbjct: 11  RRASSSPS---SHHHLHLVRALSASSPLP--ASDADLRKYAGYALLLLGCGAATYYSFPL 70

Query: 77  HENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEDLEQLEQVVKEANEKKAR 136
             +A HKKA  F+YAPLP+DLH VSNWS THEV TRV  QP+ L  L   +  A+ ++ +
Sbjct: 71  PPDALHKKAVPFKYAPLPDDLHAVSNWSATHEVHTRVLLQPDSLPVLHDALAAAHGERRK 130

Query: 137 IRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLT 196
           +RP+GSGLSPNG+ LSR GMVNLALMDKVL+VD +KK V VQAGIRV +LVD ++E+GLT
Sbjct: 131 LRPLGSGLSPNGLALSRAGMVNLALMDKVLDVDAKKKTVTVQAGIRVAELVDTLREHGLT 190

Query: 197 LQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFLL 256
           LQNFASIREQQ+GGIIQVGAHGTGA+LPPIDEQVI+MKLVTPAKGTIE+S++KDP+LF L
Sbjct: 191 LQNFASIREQQVGGIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPDLFYL 250

Query: 257 ARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDA 316
           ARCGLGGLGVVAEVTLQCVER +L+EHT++S+  ++KKNHKK L++NKH+KYL+IPYTD 
Sbjct: 251 ARCGLGGLGVVAEVTLQCVERHQLIEHTFVSSADEVKKNHKKWLSENKHIKYLWIPYTDT 310

Query: 317 IVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKY---SASEKRDINEFSFTE 376
           +VVV CNP S+WR  PKF  KY  +EAIQHVRDLY ESLKKY   + S   ++++ SFTE
Sbjct: 311 VVVVQCNPPSRWR-TPKFTSKYGKDEAIQHVRDLYRESLKKYRTKAESNDPEVDQLSFTE 370

Query: 377 LRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAG 436
           LRD+LLALDPL+K+HV+++N+AEAE+W+KSEGYR+GWSDEILGFDCGGQQWVSETCFPAG
Sbjct: 371 LRDRLLALDPLDKDHVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCFPAG 430

Query: 437 TLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGI 496
           TLAKPNMKD++Y+EEL QLIEKE+IPAPAP+EQRWTA S+SPMSPASS+ EDDIFSWVGI
Sbjct: 431 TLAKPNMKDLDYIEELLQLIEKEDIPAPAPIEQRWTACSRSPMSPASSSQEDDIFSWVGI 490

Query: 497 IMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLR 556
           IMYLPTSDARQRKEITEEFF+YR  TQT LWD YSA+EHWAKIEVPKDKDEL  L ARLR
Sbjct: 491 IMYLPTSDARQRKEITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELTELLARLR 550

Query: 557 KRFPVDEYNKARQALDPNKILSNNKLEKLF 584
           KRFPVD YNKAR  LDPNK+LSN KLEKLF
Sbjct: 551 KRFPVDAYNKARMELDPNKVLSNAKLEKLF 574

[Close]
BLAST of PI0025390 vs. ExPASy Swiss-Prot
Match: P10867 (L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3)

HSP 1 Score: 156.8 bits (395), Expect = 7.8e-37
Identity = 128/484 (26.45%), Postives = 223/484 (46.07%), Query Frame = 0 

Query: 102 NWSGTHEVWTRVFHQPEDLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLAL 161
           NW+ T+     V++QP  +E++ +V+  A E+K +++ VG G SP+ I  +   M+++  
Sbjct: 12  NWAKTYGCSPEVYYQPTSVEEVREVLALAREQKKKVKVVGGGHSPSDIACTDGFMIHMGK 71

Query: 162 MDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGA 221
           M++VL+VDKEKK++ V+AGI +  L   + E+GL + N  ++ +  + G+I  G H TG 
Sbjct: 72  MNRVLQVDKEKKQITVEAGILLADLHPQLDEHGLAMSNLGAVSDVTVAGVIGSGTHNTGI 131

Query: 222 KLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELV 281
           K   +  QV+A+ L+T     +E S+ ++ ++F  AR  LG LG++  VTLQCV +  L 
Sbjct: 132 KHGILATQVVALTLMTADGEVLECSESRNADVFQAARVHLGCLGIILTVTLQCVPQFHLQ 191

Query: 282 EHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSE 341
           E ++ S +K++  N    L  +++ ++L+ P+T+ + ++  +  +K    P     +  +
Sbjct: 192 ETSFPSTLKEVLDNLDSHLKRSEYFRFLWFPHTENVSIIYQDHTNK---APSSASNWFWD 251

Query: 342 EAIQHVRDLYVESLKKYSASEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWR 401
            AI      ++     Y       IN F F  L                        F  
Sbjct: 252 YAIGFYLLEFLLWTSTYLPCLVGWINRFFFWML------------------------FNC 311

Query: 402 KSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKE-NIPA 461
           K E   +  S +I  ++C  +Q V +        A P  K  E + ELK ++E    + A
Sbjct: 312 KKESSNL--SHKIFTYECRFKQHVQD-------WAIPREKTKEALLELKAMLEAHPKVVA 371

Query: 462 PAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQ 521
             P+E R+T      +SP           ++ IIMY P      R +          L  
Sbjct: 372 HYPVEVRFTRGDDILLSPCFQRDS----CYMNIIMYRPYGKDVPRLDY--------WLAY 431

Query: 522 TLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPV-DEYNKARQALDPNKILSNNKL 581
             +  ++    HWAK      KD          + +P   ++   R+ LDP  +  N+ L
Sbjct: 432 ETIMKKFGGRPHWAKAHNCTQKD--------FEEMYPTFHKFCDIREKLDPTGMFLNSYL 439

Query: 582 EKLF 584
           EK+F
Sbjct: 492 EKVF 439

[Close]
BLAST of PI0025390 vs. ExPASy TrEMBL
Match: E7E016 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 575/590 (97.46%), Postives = 585/590 (99.15%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVSPSPSSSS-DSELRKYLAY 60
           MLNFLSLRRS+HYFRHRRLQISPTAAISKPPFNP RPFSTVSPSPSSSS DSELRKYL Y
Sbjct: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPED 120
           GALVIFCGAATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPE+
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVK+ANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           ASE+RD+NE SFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDC
Sbjct: 361 ASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEY+EELKQLIEK+NIPAPAPLEQRWTARSKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSPA 480

Query: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           KDKDELAALQARLRKRFPVDEYNKAR+ALDPNKILSNNKLEKLFSS+DTV
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 590

[Close]
BLAST of PI0025390 vs. ExPASy TrEMBL
Match: Q9M4U9 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE=2 SV=2)

HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 565/589 (95.93%), Postives = 575/589 (97.62%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVSPSPSSSSDSELRKYLAYG 60
           MLNFLSLRRSLHYFRHRRLQISPTAAISKPP N  RPF TVSPSPSSS DSELRKYL YG
Sbjct: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYG 60

Query: 61  ALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEDL 120
           ALVIFCGAATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPEDL
Sbjct: 61  ALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDL 120

Query: 121 EQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAG 180
           EQLEQVVK+ANEKKAR+RPVGSGLSPNGIGLSR+GMVN+ALMDKVLEVDKEKKRVRVQAG
Sbjct: 121 EQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240

Query: 241 GTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300
           GTIEIS DKDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL
Sbjct: 241 GTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300

Query: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSA 360
           ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSA
Sbjct: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSA 360

Query: 361 SEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420
           SE+RDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGY+VGWSDEILGFDCG
Sbjct: 361 SEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYKVGWSDEILGFDCG 420

Query: 421 GQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPAS 480
           GQQWVSETCFPAGTLAKPNMKDIEY+EELKQLIEKENIPAPAPLEQRWT RSKSPMSPAS
Sbjct: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPAS 480

Query: 481 STAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPK 540
           STAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEVPK
Sbjct: 481 STAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVPK 540

Query: 541 DKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           DKDELAALQARLRKRFPVDE NKAR+ALDPNKILSNNKLEKLFSS D V
Sbjct: 541 DKDELAALQARLRKRFPVDESNKARRALDPNKILSNNKLEKLFSSLDMV 589

[Close]
BLAST of PI0025390 vs. ExPASy TrEMBL
Match: A0A6J1HRG8 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111465966 PE=4 SV=1)

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 549/590 (93.05%), Postives = 565/590 (95.76%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVS-PSPSSSSDSELRKYLAY 60
           ML  LSLRRSLH   HRR Q  PT AISKPP NP R FSTVS P PSSSS+SELRKYL Y
Sbjct: 1   MLKLLSLRRSLH-LHHRRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSSESELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPED 120
           GALVIFCGA TYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQP++
Sbjct: 61  GALVIFCGATTYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVHTRVFHQPDN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQL++VVKEAN KKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLQEVVKEANVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEISK+KDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYS 360

Query: 361 ASEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           AS++RDINEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFW KSEGYRVGWSDEILGFDC
Sbjct: 361 ASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWWKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEY+EELKQLIEKENIPAPAP+EQRWTA SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480

Query: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS++EDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSSSEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           KDKDELAALQARLRKRFPVDEYNKARQ LDPNKILS+NKLEKLF SSD V
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARQELDPNKILSSNKLEKLFVSSDVV 589

[Close]
BLAST of PI0025390 vs. ExPASy TrEMBL
Match: A0A6J1EEF4 (L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111432481 PE=4 SV=1)

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 545/590 (92.37%), Postives = 565/590 (95.76%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVS-PSPSSSSDSELRKYLAY 60
           ML  LSLRRSLH   HRR    PT AIS+PP NP R FSTVS P PSSSS+SELRKY+ Y
Sbjct: 1   MLRLLSLRRSLH-LHHRRPHFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGY 60

Query: 61  GALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPED 120
           GALVI CGAATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPE+
Sbjct: 61  GALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLE+VVKE+N KKARIRPVGSGLSPNGIGLSRMGMVNLALMD+VLEVDKEKKRVRVQA
Sbjct: 121 LEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEIS++KDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYS 360

Query: 361 ASEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           AS++RDINEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDC
Sbjct: 361 ASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEY+EELKQLIEKENIPAPAP+EQRWTA SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480

Query: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           KDKDELAALQARLRKRFPVDEYNKAR+ LDPNKILS+NKLEK+F SSD V
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 589

[Close]
BLAST of PI0025390 vs. ExPASy TrEMBL
Match: A0A6J1DK81 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111021307 PE=4 SV=1)

HSP 1 Score: 1040.8 bits (2690), Expect = 2.2e-300
Identity = 524/593 (88.36%), Postives = 550/593 (92.75%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLR---PFSTVSPSPSSSS-DSELRKY 60
           ML  L LRRS     HRRL   P AAIS+ P N       FSTVSP P SSS DSELRKY
Sbjct: 1   MLRILPLRRS-----HRRLLSPPAAAISRTPLNHHHHPPSFSTVSPPPPSSSFDSELRKY 60

Query: 61  LAYGALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQ 120
           L YGALV+FCGAATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVF Q
Sbjct: 61  LGYGALVVFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRVFQQ 120

Query: 121 PEDLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVR 180
           PE+LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVR
Sbjct: 121 PENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVR 180

Query: 181 VQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLV 240
           VQAGIRVQQLVDGIKE+GLTLQNFASIREQQIGGIIQVGAHGTG KLPPIDEQVIAMKLV
Sbjct: 181 VQAGIRVQQLVDGIKEHGLTLQNFASIREQQIGGIIQVGAHGTGPKLPPIDEQVIAMKLV 240

Query: 241 TPAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNH 300
           TPAKGTIEISK+KDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNM DIKKNH
Sbjct: 241 TPAKGTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMNDIKKNH 300

Query: 301 KKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLK 360
           KKLL DNKHVKYLYIPYTDA+VVVTCNPISKWRGPPK KPKYTS+EAIQHVRDLY ESLK
Sbjct: 301 KKLLTDNKHVKYLYIPYTDAVVVVTCNPISKWRGPPKLKPKYTSDEAIQHVRDLYAESLK 360

Query: 361 KYSASEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILG 420
           KYS S++ DINE+S+TELRDKLLALDPLNKEHV KVNQAEAEFWRKSEGY+VGWSDEILG
Sbjct: 361 KYSTSDETDINEYSYTELRDKLLALDPLNKEHVTKVNQAEAEFWRKSEGYKVGWSDEILG 420

Query: 421 FDCGGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPM 480
           FDCGGQQWVSETCFPAGTLAKP+MKDI+Y+E+LKQLIEKENIPAPAP+EQRWTA SKSPM
Sbjct: 421 FDCGGQQWVSETCFPAGTLAKPSMKDIKYIEDLKQLIEKENIPAPAPIEQRWTACSKSPM 480

Query: 481 SPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKI 540
           SPASS A+DD+FSWVGIIMYLPT DARQRKEITEEFFHYRHLTQ+ LWDQYSAFEHWAKI
Sbjct: 481 SPASSLAQDDLFSWVGIIMYLPTLDARQRKEITEEFFHYRHLTQSQLWDQYSAFEHWAKI 540

Query: 541 EVPKDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           EVP+DK+ELAALQARLRKRFPVDE+NKA++ALDPNKILSNNKLEKLF S+D V
Sbjct: 541 EVPRDKEELAALQARLRKRFPVDEFNKAQRALDPNKILSNNKLEKLFPSNDAV 588

[Close]
BLAST of PI0025390 vs. NCBI nr
Match: NP_001292704.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis sativus] >ADT80722.1 L-galactono-1,4-lactone dehydrogenase [Cucumis sativus])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 575/590 (97.46%), Postives = 585/590 (99.15%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVSPSPSSSS-DSELRKYLAY 60
           MLNFLSLRRS+HYFRHRRLQISPTAAISKPPFNP RPFSTVSPSPSSSS DSELRKYL Y
Sbjct: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPED 120
           GALVIFCGAATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPE+
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVK+ANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           ASE+RD+NE SFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDC
Sbjct: 361 ASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEY+EELKQLIEK+NIPAPAPLEQRWTARSKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSPA 480

Query: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           KDKDELAALQARLRKRFPVDEYNKAR+ALDPNKILSNNKLEKLFSS+DTV
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 590

[Close]
BLAST of PI0025390 vs. NCBI nr
Match: KAE8637410.1 (hypothetical protein CSA_023387 [Cucumis sativus])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 569/583 (97.60%), Postives = 579/583 (99.31%), Query Frame = 0 

Query: 8   RRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVSPSPSSSS-DSELRKYLAYGALVIFC 67
           RRS+HYFRHRRLQISPTAAISKPPFNP RPFSTVSPSPSSSS DSELRKYL YGALVIFC
Sbjct: 240 RRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGYGALVIFC 299

Query: 68  GAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEDLEQLEQV 127
           GAATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPE+LEQLEQV
Sbjct: 300 GAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPENLEQLEQV 359

Query: 128 VKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQL 187
           VK+ANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQL
Sbjct: 360 VKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAGIRVQQL 419

Query: 188 VDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEIS 247
           VDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEIS
Sbjct: 420 VDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEIS 479

Query: 248 KDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHV 307
           KDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHV
Sbjct: 480 KDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHV 539

Query: 308 KYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEKRDI 367
           KYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASE+RD+
Sbjct: 540 KYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSASEERDM 599

Query: 368 NEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVS 427
           NEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVS
Sbjct: 600 NEFSFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVS 659

Query: 428 ETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPASSTAEDD 487
           ETCFPAGTLAKPNMKDIEY+EELKQLIEK+NIPAPAPLEQRWTARSKSPMSPASSTAEDD
Sbjct: 660 ETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSPASSTAEDD 719

Query: 488 IFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELA 547
           IFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELA
Sbjct: 720 IFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPKDKDELA 779

Query: 548 ALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           ALQARLRKRFPVDEYNKAR+ALDPNKILSNNKLEKLFSS+DTV
Sbjct: 780 ALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 822

[Close]
BLAST of PI0025390 vs. NCBI nr
Match: NP_001284400.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis melo] >AAF64319.2 L-galactono-1,4-lactone dehydrogenase [Cucumis melo])

HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 565/589 (95.93%), Postives = 575/589 (97.62%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVSPSPSSSSDSELRKYLAYG 60
           MLNFLSLRRSLHYFRHRRLQISPTAAISKPP N  RPF TVSPSPSSS DSELRKYL YG
Sbjct: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVSPSPSSSFDSELRKYLGYG 60

Query: 61  ALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEDL 120
           ALVIFCGAATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPEDL
Sbjct: 61  ALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEDL 120

Query: 121 EQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAG 180
           EQLEQVVK+ANEKKAR+RPVGSGLSPNGIGLSR+GMVN+ALMDKVLEVDKEKKRVRVQAG
Sbjct: 121 EQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240

Query: 241 GTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300
           GTIEIS DKDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL
Sbjct: 241 GTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300

Query: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSA 360
           ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSA
Sbjct: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYSA 360

Query: 361 SEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420
           SE+RDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGY+VGWSDEILGFDCG
Sbjct: 361 SEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYKVGWSDEILGFDCG 420

Query: 421 GQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPAS 480
           GQQWVSETCFPAGTLAKPNMKDIEY+EELKQLIEKENIPAPAPLEQRWT RSKSPMSPAS
Sbjct: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPAS 480

Query: 481 STAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPK 540
           STAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEVPK
Sbjct: 481 STAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVPK 540

Query: 541 DKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           DKDELAALQARLRKRFPVDE NKAR+ALDPNKILSNNKLEKLFSS D V
Sbjct: 541 DKDELAALQARLRKRFPVDESNKARRALDPNKILSNNKLEKLFSSLDMV 589

[Close]
BLAST of PI0025390 vs. NCBI nr
Match: XP_023517263.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 551/590 (93.39%), Postives = 565/590 (95.76%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVS-PSPSSSSDSELRKYLAY 60
           ML  LSLRRSLH   HRR Q  PT AISKPP NP R FSTVS P PSSSS+SELRKYL Y
Sbjct: 1   MLKLLSLRRSLH-LHHRRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSSESELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPED 120
           GALVI CGAATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPE+
Sbjct: 61  GALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLE+VVKE+N KKARIRPVGSGLSPNGIGLSRMGMVNLALMD+VLEVDKEKKRVRVQA
Sbjct: 121 LEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDRVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEISKDKDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKDKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESLKKYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYS 360

Query: 361 ASEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           AS++RDINEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDC
Sbjct: 361 ASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEY+EELKQLIEKENIPAPAP+EQRWTA SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480

Query: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           KDKDELAALQARLRKRFPVDEYNKAR  LDPNKILS+NKLEKLF SSD V
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARGELDPNKILSSNKLEKLFVSSDVV 589

[Close]
BLAST of PI0025390 vs. NCBI nr
Match: XP_038882838.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 550/590 (93.22%), Postives = 564/590 (95.59%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVSPSPSSSS-DSELRKYLAY 60
           ML  L+LRRS H+ R R LQI PT AISKPP NP RPFSTVSP P SSS DSELRKYL Y
Sbjct: 1   MLKLLALRRS-HFCRLRGLQIPPTTAISKPPLNPPRPFSTVSPPPQSSSFDSELRKYLGY 60

Query: 61  GALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPED 120
           G LVI CGAATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPE+
Sbjct: 61  GTLVIICGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRVFHQPEN 120

Query: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180
           LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMD VLEVDK++KRVRVQA
Sbjct: 121 LEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDNVLEVDKDRKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEISKDKDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPK KPKYTSEEAIQHVRDLYVESLKK+S
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKLKPKYTSEEAIQHVRDLYVESLKKHS 360

Query: 361 ASEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
            S++ DINEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFW+KSEGYRVGWSDEILGFDC
Sbjct: 361 VSDETDINEFSFTELRDKLLALDPLNKEHVIKVNQAEAEFWKKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKENIPAPAPLEQRWTARSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEY+EELKQLIEKENIPAPAP+EQRWTA SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480

Query: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHL QTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLAQTLLWDQYSAFEHWAKIEVP 540

Query: 541 KDKDELAALQARLRKRFPVDEYNKARQALDPNKILSNNKLEKLFSSSDTV 590
           KDKDELAALQARLRKRFPVDEYNKAR+ALDPNKILSNNKLEKL SSSDTV
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLLSSSDTV 589

[Close]
BLAST of PI0025390 vs. TAIR 10
Match: AT3G47930.1 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 920.2 bits (2377), Expect = 8.2e-268
Identity = 461/612 (75.33%), Postives = 518/612 (84.64%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVS---------------PSP 60
           ML  L LRRS+    H    +SP+++  +  F+P R   T                 P P
Sbjct: 1   MLRSLLLRRSV---GHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRPPPPPP 60

Query: 61  SSSSDSELRKYLAYGALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSG 120
           +++S+++ RKY  Y AL IF G ATY+SFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSG
Sbjct: 61  ATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVWTRVFHQPEDLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKV 180
           THEV TR F+QPE+L  LE +VKE++EKK RIRPVGSGLSPNGIGLSR GMVNLALMDKV
Sbjct: 121 THEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPP 240
           LEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQVGAHGTGA+LPP
Sbjct: 181 LEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPP 240

Query: 241 IDEQVIAMKLVTPAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTY 300
           IDEQVI+MKLVTPAKGTIE+S++KDPELF LARCGLGGLGVVAEVTLQCV R ELVEHTY
Sbjct: 241 IDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHTY 300

Query: 301 ISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQ 360
           +SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK KPKYT++EA+Q
Sbjct: 301 VSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAVQ 360

Query: 361 HVRDLYVESLKKYSA---------SEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAE 420
           HVRDLY ES+ KY           S + DI E SFTELRDKLLALDPLN  HV KVNQAE
Sbjct: 361 HVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQAE 420

Query: 421 AEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKE 480
           AEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EY+EELK+LIEKE
Sbjct: 421 AEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEKE 480

Query: 481 NIPAPAPLEQRWTARSKSPMSPASSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYR 540
            IPAPAP+EQRWTARSKSP+SPA ST+EDDIFSWVGIIMYLPT+D RQRK+IT+EFFHYR
Sbjct: 481 AIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTADPRQRKDITDEFFHYR 540

Query: 541 HLTQTLLWDQYSAFEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARQALDPNKILSN 589
           HLTQ  LWDQ+SA+EHWAKIE+PKDK+EL ALQAR+RKRFPVD YNKAR+ LDPN+ILSN
Sbjct: 541 HLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDAYNKARRELDPNRILSN 600

[Close]
BLAST of PI0025390 vs. TAIR 10
Match: AT3G47930.2 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 729.2 bits (1881), Expect = 2.7e-210
Identity = 369/496 (74.40%), Postives = 413/496 (83.27%), Query Frame = 0 

Query: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPFNPLRPFSTVS---------------PSP 60
           ML  L LRRS+    H    +SP+++  +  F+P R   T                 P P
Sbjct: 1   MLRSLLLRRSV---GHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRPPPPPP 60

Query: 61  SSSSDSELRKYLAYGALVIFCGAATYYSFPFHENAKHKKAQIFRYAPLPEDLHTVSNWSG 120
           +++S+++ RKY  Y AL IF G ATY+SFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSG
Sbjct: 61  ATASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSG 120

Query: 121 THEVWTRVFHQPEDLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKV 180
           THEV TR F+QPE+L  LE +VKE++EKK RIRPVGSGLSPNGIGLSR GMVNLALMDKV
Sbjct: 121 THEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKV 180

Query: 181 LEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPP 240
           LEVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQVGAHGTGA+LPP
Sbjct: 181 LEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPP 240

Query: 241 IDEQVIAMKLVTPAKGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTY 300
           IDEQVI+MKLVTPAKGTIE+S++KDPELF LARCGLGGLGVVAEVTLQCV R ELVEHTY
Sbjct: 241 IDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHTY 300

Query: 301 ISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQ 360
           +SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK KPKYT++EA+Q
Sbjct: 301 VSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAVQ 360

Query: 361 HVRDLYVESLKKYSA---------SEKRDINEFSFTELRDKLLALDPLNKEHVMKVNQAE 420
           HVRDLY ES+ KY           S + DI E SFTELRDKLLALDPLN  HV KVNQAE
Sbjct: 361 HVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQAE 420

Query: 421 AEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYMEELKQLIEKE 473
           AEFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EY+EELK+LIEKE
Sbjct: 421 AEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEKE 480

[Close]
BLAST of PI0025390 vs. TAIR 10
Match: AT5G56490.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 56.6 bits (135), Expect = 7.8e-08
Identity = 34/126 (26.98%), Postives = 70/126 (55.56%), Query Frame = 0 

Query: 156 MVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVG 215
           +++   ++ V+  + E K + V++G+ ++QL++   ++ L L          +GG++  G
Sbjct: 99  LISTKFLNHVVTTNPEAKTLTVESGVTLRQLIEEAAKFDLALPYAPYWWGLTVGGMMGTG 158

Query: 216 AHGTG--AKLPPIDEQVIAMKLVTP---AKGTIEI---SKDKDPELFLLARCGLGGLGVV 274
           AHG+    K   + + V  ++LV+P   + G +++   S+  DPE F  A+  LG LGV+
Sbjct: 159 AHGSSLWGKGSAVHDYVTEIRLVSPGLASDGYVKVQVLSETIDPEEFRAAKVSLGVLGVI 218

[Close]
BLAST of PI0025390 vs. TAIR 10
Match: AT1G32300.1 (D-arabinono-1,4-lactone oxidase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.7e-07
Identity = 34/126 (26.98%), Postives = 69/126 (54.76%), Query Frame = 0 

Query: 156 MVNLALMDKVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVG 215
           +++   ++ V+  + E K + V++G+ ++QL+    E  L L +        +GG++  G
Sbjct: 100 LISTKFLNNVVGTNPEAKTLTVESGVTLRQLIGEAAELELALPHAPYWWGLTVGGLMGTG 159

Query: 216 AHGTG--AKLPPIDEQVIAMKLVTP---AKGTIEI---SKDKDPELFLLARCGLGGLGVV 274
           AHG+    K   + + V  +++V+P   + G +++   S+  DP+ F  A+  LG LGV+
Sbjct: 160 AHGSSLWGKGSAVHDYVSEIRMVSPGLASDGYVKVRVLSETIDPDEFRAAKVSLGVLGVI 219

The following BLAST results are available for this feature:
BLAST of PI0025390 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O478812.3e-26776.31L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=371... [more]
Q9SU561.2e-26675.33L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q2RAP04.1e-24072.28L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. ja... [more]
Q2QXY12.1e-23971.93L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. ja... [more]
P108677.8e-3726.45L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3[more]
BLAST of PI0025390 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
E7E0160.0e+0097.46L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Q9M4U90.0e+0095.93L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE... [more]
A0A6J1HRG80.0e+0093.05L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661... [more]
A0A6J1EEF40.0e+0092.37L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata ... [more]
A0A6J1DK812.2e-30088.36L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3... [more]
BLAST of PI0025390 vs. NCBI nr
Match NameE-valueIdentityDescription
NP_001292704.10.0e+0097.46L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis sativus] >ADT80722... [more]
KAE8637410.10.0e+0097.60hypothetical protein CSA_023387 [Cucumis sativus][more]
NP_001284400.10.0e+0095.93L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucumis melo] >AAF64319.2 ... [more]
XP_023517263.10.0e+0093.39L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo... [more]
XP_038882838.10.0e+0093.22L-galactono-1,4-lactone dehydrogenase, mitochondrial isoform X1 [Benincasa hispi... [more]
BLAST of PI0025390 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G47930.18.2e-26875.33L-galactono-1,4-lactone dehydrogenase [more]
AT3G47930.22.7e-21074.40L-galactono-1,4-lactone dehydrogenase [more]
AT5G56490.17.8e-0826.98D-arabinono-1,4-lactone oxidase family protein [more]
AT1G32300.11.7e-0726.98D-arabinono-1,4-lactone oxidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010029Galactonolactone dehydrogenaseTIGRFAMTIGR01676TIGR01676coord: 50..584
e-value: 0.0
score: 1209.2
IPR007173D-arabinono-1,4-lactone oxidasePFAMPF04030ALOcoord: 270..580
e-value: 4.1E-16
score: 59.5
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 95..163
e-value: 2.9E-12
score: 47.8
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 113..243
e-value: 5.7E-26
score: 90.9
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 165..292
e-value: 6.7E-28
score: 98.7
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePIRSFPIRSF000136LGO_GLOcoord: 95..587
e-value: 2.8E-60
score: 202.3
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePANTHERPTHR43762L-GULONOLACTONE OXIDASEcoord: 93..584
NoneNo IPR availablePANTHERPTHR43762:SF1L-GULONOLACTONE OXIDASEcoord: 93..584
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 106..277
score: 17.164734
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 94..275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0025390.1PI0025390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0016020 membrane
molecular_function GO:0003885 D-arabinono-1,4-lactone oxidase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0016633 galactonolactone dehydrogenase activity
molecular_function GO:0080049 L-gulono-1,4-lactone dehydrogenase activity
molecular_function GO:0050105 L-gulonolactone oxidase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016899 oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor