PI0025228 (gene) Melon (PI 482460) v1

BLAST of PI0025228 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 8.8e-149
Identity = 261/511 (51.08%), Postives = 354/511 (69.28%), Query Frame = 0

Query: 32  ENFLGCLSHHSPN---VSKVIYTPFNTSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 91
           ENFL C S H PN     K++YT  +  Y SILN T++NLRF +  TPKPL+I+TPS+ S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 92  HIQATVICSKSYDLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSISIEVETNTAWVQS 151
           HIQAT++CSK   LQIRTRSGGHD EG+SY++ +PF++VDL N+ SI I+V + TAWV++
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 152 GATLGELYYRIAEKNQTLAFPAGTCPIVGVGGHFSGGGYGMLMRKYGLAADNVIDAYLVD 211
           GATLGE+YY I EKN+ L+FP G CP VGVGGHFSGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 212 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVSKTWE-EGATN 271
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  T+  V K  E  G   
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 272 LIHQWQYVAHELDENV-----FLGILLAD-----KTNPTALFFSLYLGKVDELVSILNTT 331
           L ++WQ +A++ D+++     F+   + D     KT     F S++ G VD LV ++N +
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 332 FPVLGLAKKHCIETSWVESALIMGTGLQ---SGRPLEDLLSKASLTNGSTKIKSDYIKQP 391
           FP LG+ K  C E SW+++ +     +    +    E LL +++    +  IK DY+K+P
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391

Query: 392 IPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFESETPFPHRAGYLYKIGYVVNWEH 451
           IP  A+  I ++L  ED+     ++ PYGG M ++ ES  PFPHRAG +Y++ Y  +WE 
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451

Query: 452 EGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNRYGKTNYKEASVWGLK 511
           + +D EK H+ W+R +Y++ TP+VS++PR AY+NYRDLD+G  N     NY +A +WG K
Sbjct: 452 Q-EDNEK-HINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEK 511

Query: 512 YFGNNFNRLVDVKTRVDPRNFFTHEQSIPTI 526
           YFG NFNRLV VKT+VDP NFF +EQSIP +
Sbjct: 512 YFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of PI0025228 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 4.8e-147
Identity = 265/512 (51.76%), Postives = 356/512 (69.53%), Query Frame = 0

Query: 32  ENFLGCLSHHSPNVS---KVIYTPFNTSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 91
           ENFL C S + PN +   K++YT  N  Y S+LN T+ NLRF++  TPKPL+I+TPSH+S
Sbjct: 32  ENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVS 91

Query: 92  HIQATVICSKSYDLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSISIEVETNTAWVQS 151
           HIQ T++CSK   LQIRTRSGGHD EG+SY++ +PF+IVDL N+ SI I+V + TAWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEA 151

Query: 152 GATLGELYYRIAEKNQTLAFPAGTCPIVGVGGHFSGGGYGMLMRKYGLAADNVIDAYLVD 211
           GATLGE+YY + EKN+ L+  AG CP V  GGHF GGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVN 211

Query: 212 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVSKTWE-EGATN 271
            +G+  DR+SMGEDLFWA+RGGG  SFGI+VAWK++LV VP + T+  V K  E      
Sbjct: 212 VHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIMEIHELVK 271

Query: 272 LIHQWQYVAHELDENVFL-------GILLADKTNPTAL---FFSLYLGKVDELVSILNTT 331
           L+++WQ +A++ D+++ L        I      N TA+   F S++LG VD LV ++N +
Sbjct: 272 LVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKS 331

Query: 332 FPVLGLAKKHCIETSWVESALIMGTGL----QSGRPLEDLLSKASLTNGSTKIKSDYIKQ 391
           FP LG+ K  C + SW+++ +I  +G+          E LL +++  NG+ KIK DY+K+
Sbjct: 332 FPELGIKKTDCRQLSWIDT-IIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKK 391

Query: 392 PIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFESETPFPHRAGYLYKIGYVVNWE 451
           PIP      I ++L  EDI      + PYGG M ++ ES  PFPHRAG LY++ Y+ +WE
Sbjct: 392 PIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSWE 451

Query: 452 HEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNRYGKTNYKEASVWGL 511
            + +D EK HL WIR +Y++MTP+VSK+PR AY+NYRDLDIG N+     NY +A +WG 
Sbjct: 452 KQ-EDNEK-HLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWGE 511

Query: 512 KYFGNNFNRLVDVKTRVDPRNFFTHEQSIPTI 526
           KYFG NF+RLV VKT VDP NFF +EQSIP +
Sbjct: 512 KYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of PI0025228 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 5.4e-146
Identity = 257/528 (48.67%), Postives = 373/528 (70.64%), Query Frame = 0

Query: 6   LILLSLVFVVLSKSSPWVASNSSEK--YENFLGCLSHH-SPNVSKVIYTPFNTSYSSILN 65
           L+ L + F++L+ S     S S+++  +ENFL CLSH  + + S++I+T  + SY SILN
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFSILN 66

Query: 66  FTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLSYVA-Y 125
            +++N RF    TPKP+ IITP   + +Q+T+ C++ + + IRTRSGGHD+EGLSY+A  
Sbjct: 67  SSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKS 126

Query: 126 LPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVGVGGH 185
            PF+++DL NL SI+++V+  T WVQSGAT+GELYY I + +++LAFPAG  P VG+GG 
Sbjct: 127 RPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQ 186

Query: 186 FSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAW 245
           F GGGYG LMRKYGL+ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V++W
Sbjct: 187 FGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSW 246

Query: 246 KVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLADKTNPTALFFS 305
           K++L+ VP+ VT+  V KT E+ A ++I++WQY+A ++  ++F+  +L  +T   A F  
Sbjct: 247 KIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETEVYASFPG 306

Query: 306 LYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESAL--IMGTGLQSGRPLEDLLSKASL 365
           LYLG V +L++++   FP LGL   +C E SW+ES L  I G  +       ++L+K   
Sbjct: 307 LYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKGESM-------EILAKRKR 366

Query: 366 TNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFESETPFPHR 425
           T+ S K K D+I++PIP  AI+ +W+R +  +  + ++++ P+GG+MS++ ++E PFPHR
Sbjct: 367 TSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHR 426

Query: 426 AGYLYKIGYVVNW---EHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRDLDIGS 485
            G LY+I Y+  W   E + K   +++LRW+  +Y +MTP+VSKSPR AY+N+RD+D+G 
Sbjct: 427 EGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGM 486

Query: 486 NNRYG-KTNYKEASVWGLKYFGNNFNRLVDVKTRVDPRNFFTHEQSIP 524
                 KT Y+EA VWG+KYF NNF+RLV VKT VDP +FF  EQSIP
Sbjct: 487 YLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of PI0025228 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 9.1e-146
Identity = 258/524 (49.24%), Postives = 366/524 (69.85%), Query Frame = 0

Query: 4   SPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLS---HHSPNVSKVIYTPFNTSYSSI 63
           S LI  +   ++LS   P  A+ S++   +FL CLS   + S  VSKVI+TP +TS+SS+
Sbjct: 9   SVLIFFTTSTLLLSIPHPVSANRSNQ--SSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 68

Query: 64  LNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLSYVA 123
           L  +++N RFS P+ PKP+LI+TP   S +Q+ V C++ + + IRTRSGGHD+EGLSYV 
Sbjct: 69  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 128

Query: 124 YLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVGVGG 183
           + PF+I+DL NL SI+++V+  + WVQ+GAT+GELYY I +KN+TLAFPAG CP VGVGG
Sbjct: 129 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 188

Query: 184 HFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVA 243
           HFSGGGYG L+RK+GLAAD+VIDA +VDA G   +R  MGED FWAIRGGGG SF +V++
Sbjct: 189 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 248

Query: 244 WKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLADKTNPT-ALF 303
           WK+ L+ VP+TVT+  V+K  E+ A  +IH+WQ+VA ++ +++F+ ++L    N   A F
Sbjct: 249 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVRASF 308

Query: 304 FSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRPLEDLLSKASL 363
             LYLG V  L+ ++N  FP LGL +  C E SW+ES +      + G    ++L+K + 
Sbjct: 309 PGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRTR 368

Query: 364 TNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFESETPFPHR 423
            + + K KSD++++P+P  AI  +W+RL+  + E  QL+  P+GG+MS++ + ETPFPHR
Sbjct: 369 ASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHR 428

Query: 424 AGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNR 483
            G +Y+I Y+  W     DV+++++RW+  +Y  M+ FV+KSPR AYIN RDLD+G    
Sbjct: 429 KGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVG 488

Query: 484 YGKTNYKEASVWGLKYFGNNFNRLVDVKTRVDPRNFFTHEQSIP 524
             ++ Y+E   WG+KYF NNF RLV VKT VDP +FF  EQSIP
Sbjct: 489 VKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of PI0025228 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.2e-145
Identity = 258/512 (50.39%), Postives = 355/512 (69.34%), Query Frame = 0

Query: 32  ENFLGCLSHHSP-NV--SKVIYTPFNTSYSSILNFTLRNLRFSTPNTPKPLLIITPSHIS 91
           ENFL C S + P NV  +K++YT  +  Y SILN T++NLRF++  TPKPL+IITP ++S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 92  HIQATVICSKSYDLQIRTRSGGHDFEGLSYVAYLPFIIVDLINLSSISIEVETNTAWVQS 151
           HIQ T++CSK   LQIRTRSGGHD EG+SY++ +PF+IVDL N+ S+ I+V + TAWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 152 GATLGELYYRIAEKNQTLAFPAGTCPIVGVGGHFSGGGYGMLMRKYGLAADNVIDAYLVD 211
           GATLGE+YY I E N+ L+FPAG CP VG GGHFSGGGYG LMR YGLAADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 212 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTLCFVSKTWE-EGATN 271
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  T+  V K  E      
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 272 LIHQWQYVAHELDENVFLGILL----------ADKTNPTALFFSLYLGKVDELVSILNTT 331
           L+++WQ +A+  ++ + L               +KT   + F S++ G VD LV ++N +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 332 FPVLGLAKKHCIETSWVESALIMGTGLQSGRPL----EDLLSKASLTNGSTKIKSDYIKQ 391
           FP LG+ K  C + SW+++ +I  +G+ +        E LL ++     +  IK DY+K+
Sbjct: 332 FPELGIKKTDCKQLSWIDT-IIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKK 391

Query: 392 PIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFESETPFPHRAGYLYKIGYVVNWE 451
           PIP  A+  I ++L  ED+ +   +  PYGG M ++ ES  PFPHRAG +Y+I Y+ +WE
Sbjct: 392 PIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWE 451

Query: 452 HEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNRYGKTNYKEASVWGL 511
            + +D EK H+ WIR +Y++ TP+VS++PR AY+NYRDLD+G  N     NY +A +WG 
Sbjct: 452 KQ-EDNEK-HINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGE 511

Query: 512 KYFGNNFNRLVDVKTRVDPRNFFTHEQSIPTI 526
           KYFG NFNRLV VKT+VDP NFF +EQSIP +
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of PI0025228 vs. ExPASy TrEMBL
Match: A0A6J1K2F5 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490442 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 3.1e-237
Identity = 406/540 (75.19%), Postives = 464/540 (85.93%), Query Frame = 0

Query: 1   MKQSPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHS---PNVSKVIYTPFNTSY 60
           M  SPL+ LSL  +VL   S WV+SN  + ++NFL CLSH+S    ++S V+YTPFN+SY
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSY 65

Query: 61  SSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLS 120
           SS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQA V+CSKS+ LQIRTRSGGHDFEGLS
Sbjct: 66  SSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125

Query: 121 YVAYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 180
           YVA LPFIIVDLINL+SISI+VE+ TAWVQSGATLGELYYRIA+K++TLAFPAG CP VG
Sbjct: 126 YVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGICPTVG 185

Query: 181 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYGML+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFGI
Sbjct: 186 VGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGI 245

Query: 241 VVAWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLA------- 300
           VVAWKVKLVPVPA+VTLC  +KT E+GAT LIHQWQYVAH+L+ N FLGILLA       
Sbjct: 246 VVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQ 305

Query: 301 -DKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRP 360
             K NP ALFFSLYLGK DEL++ILNTTFP LGL+KK C+ETSWVE+ALIMGTGLQ G  
Sbjct: 306 GGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGSK 365

Query: 361 LEDLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQV 420
           LEDLLSKA L NGSTKIKSDYIK+PIPM AIEGIW+RL+ EDIEMPQ +  PYGG+M Q+
Sbjct: 366 LEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQI 425

Query: 421 FESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINY 480
            E ETPF HR  YLYKIGY+VNW  +G + EKRH+ WIRELYSYMTPFVSKSPRAAY+NY
Sbjct: 426 PEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNY 485

Query: 481 RDLDIGSNNRYGKTNYKEASVWGLKYFG-NNFNRLVDVKTRVDPRNFFTHEQSIPTISNK 529
           RDLDIGSNN+YG+T+Y++AS+WG KYFG  NF+RLV VKT+VDP +FF HEQSIPT+S+K
Sbjct: 486 RDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSSK 545

BLAST of PI0025228 vs. ExPASy TrEMBL
Match: A0A6J1GRE1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456783 PE=3 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 1.3e-235
Identity = 404/540 (74.81%), Postives = 462/540 (85.56%), Query Frame = 0

Query: 1   MKQSPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHS---PNVSKVIYTPFNTSY 60
           M  SPL+ LSL  +VL   S WV+SN  + ++NFL CLSH+S    ++S V+YTPFN+SY
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSY 65

Query: 61  SSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLS 120
           SS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQA V+CSKS+ LQIRTRSGGHDFEGLS
Sbjct: 66  SSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125

Query: 121 YVAYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 180
           YVA LPFIIVDLINL+SISI+VE  TAWVQSGATLGELYY IA+K+QTLAFPAG CP VG
Sbjct: 126 YVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVG 185

Query: 181 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYGML+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFGI
Sbjct: 186 VGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFGI 245

Query: 241 VVAWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLA------- 300
           VVAWKVKLVPVPA+VTLC  +KT E+GAT LIHQWQYVAH+L+ N FLGILLA       
Sbjct: 246 VVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQ 305

Query: 301 -DKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRP 360
             K NP ALFFSLYLGK +EL++ILNTTFP LGL+KK C+ETSWVE+ALIMGTGLQ G  
Sbjct: 306 GGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGSK 365

Query: 361 LEDLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQV 420
           LEDLLSKA+L NGSTKIKSDYIK+PIPM AIEGIW+RL+ EDIEMPQ +  PYGG+M Q+
Sbjct: 366 LEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQI 425

Query: 421 FESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINY 480
            E ETPF HR   LYKIGY+VNW  +G + EKRH+ WIRELYSYMTPFVSKSPRAAY+NY
Sbjct: 426 PEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNY 485

Query: 481 RDLDIGSNNRYGKTNYKEASVWGLKYFG-NNFNRLVDVKTRVDPRNFFTHEQSIPTISNK 529
           RDLDIGSNN+YG+T+Y++AS+WG KYFG  NF+RLV VKT+VDP +FF HEQSIPT+S+K
Sbjct: 486 RDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSSK 545

BLAST of PI0025228 vs. ExPASy TrEMBL
Match: A0A6J1GRG1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456798 PE=3 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 3.0e-232
Identity = 398/544 (73.16%), Postives = 461/544 (84.74%), Query Frame = 0

Query: 1   MKQSPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHS---PNVSKVIYTPFNTSY 60
           M  SPL+ LSL  +VL   S WV++N  + +ENFL CLSH+S    ++S V+YTPFN+SY
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSWVSANFGKPHENFLQCLSHYSQDGSSISNVLYTPFNSSY 65

Query: 61  SSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLS 120
           S++LNFTLRNLRFSTP+TPKPLLIITP H+SHIQA V+CSKS+ LQIRTRSGGHDFEGLS
Sbjct: 66  SNVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125

Query: 121 YVAYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 180
           YVA LPFIIVDLINL+SISI+V++ TAWVQSGATLGELYY IA+K+QTL FPAG CP VG
Sbjct: 126 YVASLPFIIVDLINLNSISIDVKSKTAWVQSGATLGELYYSIAKKSQTLTFPAGICPTVG 185

Query: 181 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYG L+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFGI
Sbjct: 186 VGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGI 245

Query: 241 VVAWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILL-------- 300
           VVAWKVKLV VPA+VTLC V+KT E+GAT LIHQWQYVAH+L+ ++FLGILL        
Sbjct: 246 VVAWKVKLVSVPASVTLCMVNKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGGNFTRQ 305

Query: 301 ADKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGT----GLQ 360
            DK NP ALFFSLYLGK DELV+ILNTTFP LGL+KK C+ETSWVE+A+IMGT    GLQ
Sbjct: 306 GDKINPVALFFSLYLGKADELVAILNTTFPELGLSKKDCMETSWVETAIIMGTGLQLGLQ 365

Query: 361 SGRPLEDLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGR 420
            G  LEDLLSK +    STKIKSDY+K+PIPM AIEGIW+RL+ +DIE+PQ +  PYGGR
Sbjct: 366 PGSKLEDLLSKTTPIIASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQYIFVPYGGR 425

Query: 421 MSQVFESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAA 480
           M Q+ ESETPF HR  Y+YKIGYVV W  +G D EKRH+ WIRELYSYMTPFVSKSPRAA
Sbjct: 426 MGQISESETPFSHRGEYMYKIGYVVGWVEQGIDAEKRHIDWIRELYSYMTPFVSKSPRAA 485

Query: 481 YINYRDLDIGSNNRYGKTNYKEASVWGLKYFGN-NFNRLVDVKTRVDPRNFFTHEQSIPT 529
           Y+NYRDLDIGSNN+YG+T+Y++AS+WG KYFGN NF+RLV VKT+VDP +FF HEQSIPT
Sbjct: 486 YLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGNENFDRLVHVKTKVDPYDFFRHEQSIPT 545

BLAST of PI0025228 vs. ExPASy TrEMBL
Match: A0A6J1GTA5 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456788 PE=3 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 2.0e-228
Identity = 391/540 (72.41%), Postives = 448/540 (82.96%), Query Frame = 0

Query: 1   MKQSPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHS---PNVSKVIYTPFNTSY 60
           M  SP +  SL F+V    S WV+SN ++ +ENFL CLSH+S    ++S V+YTPFN+SY
Sbjct: 6   MNSSPFLPFSLTFLVFCSCSSWVSSNFAKPHENFLQCLSHYSQDASSISNVLYTPFNSSY 65

Query: 61  SSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLS 120
           S +LNFTLRNLRFSTP+TPKPLLIITP H+SHIQA V+CSKS+ LQIRTRSGGHD EGLS
Sbjct: 66  SDVLNFTLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDIEGLS 125

Query: 121 YVAYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 180
           YVA LPFIIVDLINL+SISI+VE+ TAWVQSGATLGELYYRIAEK+++LAFPAG CP VG
Sbjct: 126 YVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAEKSRSLAFPAGICPTVG 185

Query: 181 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGH SGGGYG+L+RKYGLAADNVIDA LVDANG+F DR+ MGEDLFWAIRGGGGGSFGI
Sbjct: 186 VGGHLSGGGYGLLLRKYGLAADNVIDAQLVDANGKFFDRKLMGEDLFWAIRGGGGGSFGI 245

Query: 241 VVAWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILL-------- 300
           VVAWKV LV VPATVTLC V+KT E+GAT LIHQWQYVAH+L+ N+FL ILL        
Sbjct: 246 VVAWKVNLVSVPATVTLCVVNKTVEDGATKLIHQWQYVAHKLENNLFLSILLEGGNFTRQ 305

Query: 301 ADKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRP 360
             K NP ALFFSLYLGK DEL++ILNTTFP LGL+K  C+ETSWVE+ALIMGTGLQ G  
Sbjct: 306 GSKINPLALFFSLYLGKADELLTILNTTFPELGLSKTDCMETSWVETALIMGTGLQPGSK 365

Query: 361 LEDLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQV 420
           LEDLLSK  L    TK+KSDY+K+PIPM AIEGIWQRL+  DIE PQ++  PYGG+MS++
Sbjct: 366 LEDLLSKTPLMTVRTKMKSDYVKEPIPMAAIEGIWQRLRSGDIETPQIIFIPYGGKMSEI 425

Query: 421 FESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINY 480
            E ETPF HR  Y YKIGY  NW   G D E +HL WIRELYSYM PFVSKSPRAAY+NY
Sbjct: 426 SELETPFSHRGEYSYKIGYTTNWIEGGIDAENKHLNWIRELYSYMAPFVSKSPRAAYLNY 485

Query: 481 RDLDIGSNNRYGKTNYKEASVWGLKYFG-NNFNRLVDVKTRVDPRNFFTHEQSIPTISNK 529
           RDLDIGSNN+YG+TNY++AS+WG KYFG  NF+RLV VKT+VDP +FF HEQSIPT+S+K
Sbjct: 486 RDLDIGSNNKYGRTNYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSSK 545

BLAST of PI0025228 vs. ExPASy TrEMBL
Match: A0A6J1K3Z1 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490441 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 5.8e-228
Identity = 394/544 (72.43%), Postives = 453/544 (83.27%), Query Frame = 0

Query: 1   MKQSPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHS---PNVSKVIYTPFNTSY 60
           M  SPL+ LSL  +VL   S WV+SN  + ++NFL CLSH+S    ++S V+YTPFN+SY
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSY 65

Query: 61  SSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLS 120
           SS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQA V+CSKS+ LQIRTRSGGHDFEGLS
Sbjct: 66  SSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125

Query: 121 YVAYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 180
           YVA LPFIIVDLI L+SISI+VE+ TAWVQSGATLGELYY IA+K+QTLAFPAG CP VG
Sbjct: 126 YVASLPFIIVDLIKLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVG 185

Query: 181 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYG L+RKYGLAADNVIDA LVDANG+F +RE MGEDLFWAIRGGGGGSFGI
Sbjct: 186 VGGHFSGGGYGRLLRKYGLAADNVIDAQLVDANGKFVNRELMGEDLFWAIRGGGGGSFGI 245

Query: 241 VVAWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILL-------- 300
           VVAWKV LV VPA+VTLC  SKT E+GAT LIHQWQYVAH+L+ ++FLGILL        
Sbjct: 246 VVAWKVNLVSVPASVTLCMASKTSEDGATKLIHQWQYVAHKLENDIFLGILLGGGNFSRQ 305

Query: 301 ADKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGT----GLQ 360
            DK NP ALFFSLYLGK DELV+IL+T FP LGL+KK C+ETSWVE+A+IMGT    GLQ
Sbjct: 306 GDKINPVALFFSLYLGKADELVAILDTKFPELGLSKKDCMETSWVETAIIMGTGLQPGLQ 365

Query: 361 SGRPLEDLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGR 420
            G  LEDL SK +  N STKIKSDY+K+PIPM AIEGIW+RL+ +DIE+PQ L  PYGGR
Sbjct: 366 PGSKLEDLQSKTTPINASTKIKSDYVKEPIPMAAIEGIWERLRSQDIELPQHLFVPYGGR 425

Query: 421 MSQVFESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAA 480
           M Q+ E ETPF HR  Y+YKIGY V W   G D EKRH+ WIRE+YSYMTPFVSKSPRAA
Sbjct: 426 MGQIQEFETPFSHRGEYMYKIGYTVGWVEGGIDAEKRHIDWIREIYSYMTPFVSKSPRAA 485

Query: 481 YINYRDLDIGSNNRYGKTNYKEASVWGLKYFG-NNFNRLVDVKTRVDPRNFFTHEQSIPT 529
           Y+NYRDLDIGSNN+YG+T+Y++AS+WG KYFG  NF+RLV VKT+VDP +FF HEQSIPT
Sbjct: 486 YLNYRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPT 545

BLAST of PI0025228 vs. NCBI nr
Match: XP_038895570.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 966.8 bits (2498), Expect = 7.4e-278
Identity = 474/539 (87.94%), Postives = 500/539 (92.76%), Query Frame = 0

Query: 1   MKQSPLI-LLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHSPNVSKVIYTPFNTSYSS 60
           MK SPLI LLSLV ++LS S+PWVASNSSEK+ENFL CL HHSPN+SKVIYTPFN+SYSS
Sbjct: 1   MKSSPLILLLSLVVILLSSSAPWVASNSSEKHENFLECLFHHSPNISKVIYTPFNSSYSS 60

Query: 61  ILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLSYV 120
           ILNFTLRNLRFSTPNTPKPLLIITPSHISHIQ  VICSKSY LQIRTRSGGHDFEGLSYV
Sbjct: 61  ILNFTLRNLRFSTPNTPKPLLIITPSHISHIQVAVICSKSYGLQIRTRSGGHDFEGLSYV 120

Query: 121 AYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVGVG 180
           AYLPFIIVDLINLSSI IE+ETNTAWV+SGATLGELYYRIAEK++TLAFPAGTCPIVGVG
Sbjct: 121 AYLPFIIVDLINLSSIQIEIETNTAWVESGATLGELYYRIAEKSRTLAFPAGTCPIVGVG 180

Query: 181 GHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 240
           GH SGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV
Sbjct: 181 GHLSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 240

Query: 241 AWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLA--------D 300
           AWKVKLV VPATVTLCFVSKTWEEGATNLIH+WQYVAH+LDEN+F+GILL+         
Sbjct: 241 AWKVKLVLVPATVTLCFVSKTWEEGATNLIHRWQYVAHDLDENLFIGILLSGGNFTRQEG 300

Query: 301 KTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRPLE 360
           KTNPTALFFSLYLGKVDELVSILN TFP LGLAKK CIETSWVESALIMGTGLQ G+ LE
Sbjct: 301 KTNPTALFFSLYLGKVDELVSILNKTFPELGLAKKDCIETSWVESALIMGTGLQPGKSLE 360

Query: 361 DLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFE 420
           DLLSKASLTNG+TKIKSDYIKQPIP+ A+EGIWQRLKLEDIEMPQLL APYGGRMSQ+ E
Sbjct: 361 DLLSKASLTNGNTKIKSDYIKQPIPLAAVEGIWQRLKLEDIEMPQLLFAPYGGRMSQISE 420

Query: 421 SETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRD 480
           SE+PF HRAGYLYKIGYV  WE +  DVEKRHL WIRELYSYMTPFVSKSPRAAYINYRD
Sbjct: 421 SESPFSHRAGYLYKIGYVTYWEQQDNDVEKRHLSWIRELYSYMTPFVSKSPRAAYINYRD 480

Query: 481 LDIGSNNRYGKTNYKEASVWGLKYFGNNFNRLVDVKTRVDPRNFFTHEQSIPTISNKKV 531
           LDIGSNN+YGKT+YK+AS+WGLKYFGNNFNRLVDVKTRVDP NFF HEQSIPT SNKKV
Sbjct: 481 LDIGSNNKYGKTSYKQASIWGLKYFGNNFNRLVDVKTRVDPHNFFRHEQSIPTFSNKKV 539

BLAST of PI0025228 vs. NCBI nr
Match: XP_022994835.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 830.9 bits (2145), Expect = 6.3e-237
Identity = 406/540 (75.19%), Postives = 464/540 (85.93%), Query Frame = 0

Query: 1   MKQSPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHS---PNVSKVIYTPFNTSY 60
           M  SPL+ LSL  +VL   S WV+SN  + ++NFL CLSH+S    ++S V+YTPFN+SY
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSY 65

Query: 61  SSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLS 120
           SS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQA V+CSKS+ LQIRTRSGGHDFEGLS
Sbjct: 66  SSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125

Query: 121 YVAYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 180
           YVA LPFIIVDLINL+SISI+VE+ TAWVQSGATLGELYYRIA+K++TLAFPAG CP VG
Sbjct: 126 YVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYRIAKKSRTLAFPAGICPTVG 185

Query: 181 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYGML+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFGI
Sbjct: 186 VGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKLMGEDLFWAIRGGGGGSFGI 245

Query: 241 VVAWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLA------- 300
           VVAWKVKLVPVPA+VTLC  +KT E+GAT LIHQWQYVAH+L+ N FLGILLA       
Sbjct: 246 VVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQ 305

Query: 301 -DKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRP 360
             K NP ALFFSLYLGK DEL++ILNTTFP LGL+KK C+ETSWVE+ALIMGTGLQ G  
Sbjct: 306 GGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGSK 365

Query: 361 LEDLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQV 420
           LEDLLSKA L NGSTKIKSDYIK+PIPM AIEGIW+RL+ EDIEMPQ +  PYGG+M Q+
Sbjct: 366 LEDLLSKAPLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQI 425

Query: 421 FESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINY 480
            E ETPF HR  YLYKIGY+VNW  +G + EKRH+ WIRELYSYMTPFVSKSPRAAY+NY
Sbjct: 426 PEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNY 485

Query: 481 RDLDIGSNNRYGKTNYKEASVWGLKYFG-NNFNRLVDVKTRVDPRNFFTHEQSIPTISNK 529
           RDLDIGSNN+YG+T+Y++AS+WG KYFG  NF+RLV VKT+VDP +FF HEQSIPT+S+K
Sbjct: 486 RDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSSK 545

BLAST of PI0025228 vs. NCBI nr
Match: KAG6573426.1 (Cannabidiolic acid synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 828.9 bits (2140), Expect = 2.4e-236
Identity = 405/540 (75.00%), Postives = 463/540 (85.74%), Query Frame = 0

Query: 1   MKQSPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHS---PNVSKVIYTPFNTSY 60
           M  S L+ LSL  +VL   S WV+SN  + ++NFL CLSH+S    ++S V+YTPFN++Y
Sbjct: 6   MNSSSLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSTY 65

Query: 61  SSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLS 120
           SS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQA V+CSKS+ LQIRTRSGGHDFEGLS
Sbjct: 66  SSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125

Query: 121 YVAYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 180
           YVA LPFIIVDLINL+SISI+VE+ TAWVQSGATLGELYY IA+K+QTLAFPAG CP VG
Sbjct: 126 YVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVG 185

Query: 181 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYGML+RKYGLAADNVIDA LVDANG+F DR+ MGEDLFWAIRGGGGGSFGI
Sbjct: 186 VGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVDRKLMGEDLFWAIRGGGGGSFGI 245

Query: 241 VVAWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLA------- 300
           VVAWKVKLVPVPA+VTLC  +KT E+GAT LIHQWQYVAH+L+ N FLGILLA       
Sbjct: 246 VVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQ 305

Query: 301 -DKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRP 360
             K NP ALFFSLYLGK DEL++ILNTTFP LGL+KK C+ETSWVE+ALIMGTGLQ G  
Sbjct: 306 GGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCLETSWVETALIMGTGLQFGSK 365

Query: 361 LEDLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQV 420
           LEDLLSKA+L NGSTKIKSDYIK+PIPM AIEGIW+RL+ EDIEMPQ +  PYGG+M Q+
Sbjct: 366 LEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQI 425

Query: 421 FESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINY 480
            E ETPF HR  YLYKIGY+VNW  +G + EKRH+ WIRELYSYMTPFVSKSPRAAY+NY
Sbjct: 426 PEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNY 485

Query: 481 RDLDIGSNNRYGKTNYKEASVWGLKYFG-NNFNRLVDVKTRVDPRNFFTHEQSIPTISNK 529
           RDLDIGSNN+YG+T+Y++AS+WG KYFG  NF+RLV VKT+VDP +FF HEQSIPT+S+K
Sbjct: 486 RDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSSK 545

BLAST of PI0025228 vs. NCBI nr
Match: XP_022954543.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 825.5 bits (2131), Expect = 2.7e-235
Identity = 404/540 (74.81%), Postives = 462/540 (85.56%), Query Frame = 0

Query: 1   MKQSPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHS---PNVSKVIYTPFNTSY 60
           M  SPL+ LSL  +VL   S WV+SN  + ++NFL CLSH+S    ++S V+YTPFN+SY
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSWVSSNFVKPHQNFLQCLSHYSQDGSSISDVLYTPFNSSY 65

Query: 61  SSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLS 120
           SS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQA V+CSKS+ LQIRTRSGGHDFEGLS
Sbjct: 66  SSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125

Query: 121 YVAYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 180
           YVA LPFIIVDLINL+SISI+VE  TAWVQSGATLGELYY IA+K+QTLAFPAG CP VG
Sbjct: 126 YVASLPFIIVDLINLNSISIDVENKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVG 185

Query: 181 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYGML+RKYGLAADNVIDA LVDANG+F +R+ MGEDLFWAIRGGGGGSFGI
Sbjct: 186 VGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRQLMGEDLFWAIRGGGGGSFGI 245

Query: 241 VVAWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLA------- 300
           VVAWKVKLVPVPA+VTLC  +KT E+GAT LIHQWQYVAH+L+ N FLGILLA       
Sbjct: 246 VVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTRQ 305

Query: 301 -DKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRP 360
             K NP ALFFSLYLGK +EL++ILNTTFP LGL+KK C+ETSWVE+ALIMGTGLQ G  
Sbjct: 306 GGKINPLALFFSLYLGKANELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQLGSK 365

Query: 361 LEDLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQV 420
           LEDLLSKA+L NGSTKIKSDYIK+PIPM AIEGIW+RL+ EDIEMPQ +  PYGG+M Q+
Sbjct: 366 LEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQI 425

Query: 421 FESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINY 480
            E ETPF HR   LYKIGY+VNW  +G + EKRH+ WIRELYSYMTPFVSKSPRAAY+NY
Sbjct: 426 PEFETPFSHRGENLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLNY 485

Query: 481 RDLDIGSNNRYGKTNYKEASVWGLKYFG-NNFNRLVDVKTRVDPRNFFTHEQSIPTISNK 529
           RDLDIGSNN+YG+T+Y++AS+WG KYFG  NF+RLV VKT+VDP +FF HEQSIPT+S+K
Sbjct: 486 RDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSSK 545

BLAST of PI0025228 vs. NCBI nr
Match: XP_023542525.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 823.9 bits (2127), Expect = 7.8e-235
Identity = 404/541 (74.68%), Postives = 464/541 (85.77%), Query Frame = 0

Query: 1   MKQSPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHS---PNVSKVIYTPFNTSY 60
           M  SPL+ LSL  +VL   S WV++N  + +++FL CLSH+S    ++S V+YTPFN+SY
Sbjct: 6   MNSSPLLPLSLTLLVLCSCSSWVSANFVKPHQSFLQCLSHYSQDASSISDVLYTPFNSSY 65

Query: 61  SSILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLS 120
           SS+LN TLRNLRFSTP+TPKPLLIITP H+SHIQA V+CSKS+ LQIRTRSGGHDFEGLS
Sbjct: 66  SSVLNITLRNLRFSTPHTPKPLLIITPLHVSHIQAAVLCSKSHGLQIRTRSGGHDFEGLS 125

Query: 121 YVAYLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 180
           YVA LPFIIVDLINL+SISI+VE+ TAWVQSGATLGELYY IA+K+QTLAFPAG CP VG
Sbjct: 126 YVASLPFIIVDLINLNSISIDVESKTAWVQSGATLGELYYSIAKKSQTLAFPAGICPTVG 185

Query: 181 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRES-MGEDLFWAIRGGGGGSFG 240
           VGGHFSGGGYGML+RKYGLAADNVIDA LVDANG+F +R+  MGEDLFWAIRGGGGGSFG
Sbjct: 186 VGGHFSGGGYGMLLRKYGLAADNVIDAQLVDANGKFVNRKKLMGEDLFWAIRGGGGGSFG 245

Query: 241 IVVAWKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLA------ 300
           IVVAWKVKLVPVPA+VTLC  +KT E+GAT LIHQWQYVAH+L+ N FLGILLA      
Sbjct: 246 IVVAWKVKLVPVPASVTLCMANKTSEDGATKLIHQWQYVAHKLENNAFLGILLAGGNFTR 305

Query: 301 --DKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGR 360
              K NP ALFFSLYLGK DEL++ILNTTFP LGL+KK C+ETSWVE+ALIMGTGLQ G 
Sbjct: 306 QGGKINPLALFFSLYLGKADELLTILNTTFPELGLSKKDCMETSWVETALIMGTGLQFGS 365

Query: 361 PLEDLLSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQ 420
            LEDLLSKA+L NGSTKIKSDYIK+PIPM AIEGIW+RL+ EDIEMPQ +  PYGG+M Q
Sbjct: 366 KLEDLLSKATLVNGSTKIKSDYIKEPIPMAAIEGIWERLRYEDIEMPQHIFVPYGGKMGQ 425

Query: 421 VFESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYIN 480
           + E ETPF HR  YLYKIGY+VNW  +G + EKRH+ WIRELYSYMTPFVSKSPRAAY+N
Sbjct: 426 IPEFETPFSHRGEYLYKIGYIVNWAEQGIEAEKRHIDWIRELYSYMTPFVSKSPRAAYLN 485

Query: 481 YRDLDIGSNNRYGKTNYKEASVWGLKYFG-NNFNRLVDVKTRVDPRNFFTHEQSIPTISN 529
           YRDLDIGSNN+YG+T+Y++AS+WG KYFG  NF+RLV VKT+VDP +FF HEQSIPT+S+
Sbjct: 486 YRDLDIGSNNKYGRTSYEQASIWGYKYFGKENFDRLVHVKTKVDPYDFFRHEQSIPTLSS 545

BLAST of PI0025228 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 519.2 bits (1336), Expect = 3.8e-147
Identity = 257/528 (48.67%), Postives = 373/528 (70.64%), Query Frame = 0

Query: 6   LILLSLVFVVLSKSSPWVASNSSEK--YENFLGCLSHH-SPNVSKVIYTPFNTSYSSILN 65
           L+ L + F++L+ S     S S+++  +ENFL CLSH  + + S++I+T  + SY SILN
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFSILN 66

Query: 66  FTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLSYVA-Y 125
            +++N RF    TPKP+ IITP   + +Q+T+ C++ + + IRTRSGGHD+EGLSY+A  
Sbjct: 67  SSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKS 126

Query: 126 LPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVGVGGH 185
            PF+++DL NL SI+++V+  T WVQSGAT+GELYY I + +++LAFPAG  P VG+GG 
Sbjct: 127 RPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQ 186

Query: 186 FSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAW 245
           F GGGYG LMRKYGL+ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V++W
Sbjct: 187 FGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSW 246

Query: 246 KVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLADKTNPTALFFS 305
           K++L+ VP+ VT+  V KT E+ A ++I++WQY+A ++  ++F+  +L  +T   A F  
Sbjct: 247 KIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETEVYASFPG 306

Query: 306 LYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESAL--IMGTGLQSGRPLEDLLSKASL 365
           LYLG V +L++++   FP LGL   +C E SW+ES L  I G  +       ++L+K   
Sbjct: 307 LYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKGESM-------EILAKRKR 366

Query: 366 TNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFESETPFPHR 425
           T+ S K K D+I++PIP  AI+ +W+R +  +  + ++++ P+GG+MS++ ++E PFPHR
Sbjct: 367 TSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHR 426

Query: 426 AGYLYKIGYVVNW---EHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRDLDIGS 485
            G LY+I Y+  W   E + K   +++LRW+  +Y +MTP+VSKSPR AY+N+RD+D+G 
Sbjct: 427 EGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGM 486

Query: 486 NNRYG-KTNYKEASVWGLKYFGNNFNRLVDVKTRVDPRNFFTHEQSIP 524
                 KT Y+EA VWG+KYF NNF+RLV VKT VDP +FF  EQSIP
Sbjct: 487 YLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of PI0025228 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 518.5 bits (1334), Expect = 6.5e-147
Identity = 258/524 (49.24%), Postives = 366/524 (69.85%), Query Frame = 0

Query: 4   SPLILLSLVFVVLSKSSPWVASNSSEKYENFLGCLS---HHSPNVSKVIYTPFNTSYSSI 63
           S LI  +   ++LS   P  A+ S++   +FL CLS   + S  VSKVI+TP +TS+SS+
Sbjct: 9   SVLIFFTTSTLLLSIPHPVSANRSNQ--SSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSV 68

Query: 64  LNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLSYVA 123
           L  +++N RFS P+ PKP+LI+TP   S +Q+ V C++ + + IRTRSGGHD+EGLSYV 
Sbjct: 69  LASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVT 128

Query: 124 YLPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVGVGG 183
           + PF+I+DL NL SI+++V+  + WVQ+GAT+GELYY I +KN+TLAFPAG CP VGVGG
Sbjct: 129 HKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGG 188

Query: 184 HFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVA 243
           HFSGGGYG L+RK+GLAAD+VIDA +VDA G   +R  MGED FWAIRGGGG SF +V++
Sbjct: 189 HFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLS 248

Query: 244 WKVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILLADKTNPT-ALF 303
           WK+ L+ VP+TVT+  V+K  E+ A  +IH+WQ+VA ++ +++F+ ++L    N   A F
Sbjct: 249 WKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRYKNMVRASF 308

Query: 304 FSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRPLEDLLSKASL 363
             LYLG V  L+ ++N  FP LGL +  C E SW+ES +      + G    ++L+K + 
Sbjct: 309 PGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRTR 368

Query: 364 TNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFESETPFPHR 423
            + + K KSD++++P+P  AI  +W+RL+  + E  QL+  P+GG+MS++ + ETPFPHR
Sbjct: 369 ASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHR 428

Query: 424 AGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRDLDIGSNNR 483
            G +Y+I Y+  W     DV+++++RW+  +Y  M+ FV+KSPR AYIN RDLD+G    
Sbjct: 429 KGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVG 488

Query: 484 YGKTNYKEASVWGLKYFGNNFNRLVDVKTRVDPRNFFTHEQSIP 524
             ++ Y+E   WG+KYF NNF RLV VKT VDP +FF  EQSIP
Sbjct: 489 VKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of PI0025228 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 2.0e-143
Identity = 260/533 (48.78%), Postives = 358/533 (67.17%), Query Frame = 0

Query: 7   ILLSLVFVVLSKSSPWVASNSSEKYENFLGCLSHHSP---NVSKVIYTPFNTSYSSILNF 66
           ++L L FVV      W +S+SS   E F  CL+ +S     +S  I+   N SYSS+L  
Sbjct: 5   LILVLFFVVFI----WQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQA 64

Query: 67  TLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLSYVAY-- 126
            +RNLRF+T +TPKP LII  +H SH+QA + C K ++LQ++ RSGGHD++GLSYV Y  
Sbjct: 65  NIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSG 124

Query: 127 LPFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVGVGGH 186
            PF ++D+ NL S+ ++V + TAWVQ+GA LGE+YY I EK++TLA+PAG CP VGVGGH
Sbjct: 125 KPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGH 184

Query: 187 FSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAW 246
            SGGGYG +MRKYGL  DN IDA +VD NG+  DR+ MGEDL+WAI GGGGGS+G+V+A+
Sbjct: 185 ISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAY 244

Query: 247 KVKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFL--------GILLADKT 306
           K+ LV VP  VT+  +S+T E+ AT++IH+WQ VA +L + +F+        G + + KT
Sbjct: 245 KINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKT 304

Query: 307 NPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRPLEDL 366
             T  F +++LG    L+SILN  FP LGL +  C ETSW++S L   T +Q G   E L
Sbjct: 305 VRTT-FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFW-TNIQVGSS-ETL 364

Query: 367 LSKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFESE 426
           L + +      K KSDY+++PI    +E IW+  K+ ++E+P +   PYGG M ++  + 
Sbjct: 365 LLQRNQPVNYLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTV 424

Query: 427 TPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRDLD 486
           TPFP+RAG L+KI Y  NW  E   +  R++   R+LY +MTPFVSK+PR ++ NYRD+D
Sbjct: 425 TPFPYRAGNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVD 484

Query: 487 IGSNNRYGK-TNYKEASVWGLKYFGNNFNRLVDVKTRVDPRNFFTHEQSIPTI 526
           +G N+  GK ++Y E   +G KYF  NF RLV +KTRVD  NFF +EQSIP +
Sbjct: 485 LGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of PI0025228 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 506.5 bits (1303), Expect = 2.6e-143
Identity = 256/529 (48.39%), Postives = 365/529 (69.00%), Query Frame = 0

Query: 10  SLVFVVLSKS---SPWVASNSSEKYENFLGCLSHHSPNVS---KVIYTPFNTSYSSILNF 69
           S +F +L  S   SP V+++ S  +E+FL CLS+   + +   KVI+T  ++S+ SIL+ 
Sbjct: 5   SFLFTILLFSLNISPLVSAHGS-NHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDS 64

Query: 70  TLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLSYVAY-L 129
           +++N RFS   TPKP+ IITP   S +Q  + C++ + + +RTRS GH +EGLSY+AY  
Sbjct: 65  SIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNK 124

Query: 130 PFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVGVGGHF 189
           PF ++DL NL SIS++V+  T WVQ+GAT GELYY I +  ++LAFPAG  P VGVGG F
Sbjct: 125 PFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQF 184

Query: 190 SGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWK 249
           SGGGYG L+RKYGLAADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WK
Sbjct: 185 SGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWK 244

Query: 250 VKLVPVPATVTLCFVSKTWEEGATNLIHQWQYVAHELDENVFLGILL--ADKTNPTALFF 309
           VKLV VP+T+T+  V KT ++ A  +I +WQY A ++ +++F+   L  ++K    ALF 
Sbjct: 245 VKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKNAVHALFT 304

Query: 310 SLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRPLEDLLSKASLT 369
            LY+G V+ L++++   FP LGL K+ C E SW+ES L        G  L  +L+    T
Sbjct: 305 GLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWF-ADFPKGESL-GVLTNRERT 364

Query: 370 NGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQVFESETPFPHRA 429
           + S K K D++++PIP  AI+ IW+RL+  +  + ++++ P+GG+MS++ E ETPFPHR 
Sbjct: 365 SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRG 424

Query: 430 GYLYKIGYVVNW---EHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINYRDLDIGSN 489
           G LY+I YV  W   E + K    ++L+W+  +Y +MTP+VSKSPR AY+N++D+D+G  
Sbjct: 425 GNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMY 484

Query: 490 NRYGKTNYKEASVWGLKYFGNNFNRLVDVKTRVDPRNFFTHEQSIPTIS 527
               KT Y+E   WG+KYF NNF RLV VKTRVDP +FF  EQSIP ++
Sbjct: 485 LGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of PI0025228 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 501.5 bits (1290), Expect = 8.2e-142
Identity = 258/534 (48.31%), Postives = 358/534 (67.04%), Query Frame = 0

Query: 5   PLILLSLVFVVLSKSSPWVASNSSEKYENFLGCL---SHHSPNVSKVIYTPFN--TSYSS 64
           PL L S++ +  S  +    S+ +   + F+ C+   +H    + K  + P    + +S 
Sbjct: 6   PLPLFSILVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQ 65

Query: 65  ILNFTLRNLRFSTPNTPKPLLIITPSHISHIQATVICSKSYDLQIRTRSGGHDFEGLSYV 124
           +L  T +NLRF   + PKP  I +P H SH+QA++ICSK   + +R RSGGHD+EGLSYV
Sbjct: 66  VLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYV 125

Query: 125 AYL--PFIIVDLINLSSISIEVETNTAWVQSGATLGELYYRIAEKNQTLAFPAGTCPIVG 184
           + +  PFI++DL  +  ++I ++ N+AWVQSGAT+GELYYRIAEK++   FPAG C  +G
Sbjct: 126 SQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLG 185

Query: 185 VGGHFSGGGYGMLMRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 244
           +GGH +GG YG +MRKYGL ADNV+DA +VDANG+  DR +MGED FWAIRGG GGSFGI
Sbjct: 186 IGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGI 245

Query: 245 VVAWKVKLVPVPATVTLCFVSKTWEEGATN-LIHQWQYVAHELDENVFLGILL------A 304
           ++AWK+KLVPVP TVT+  V+KT ++   N +I +WQ VA +L E +F+ +L        
Sbjct: 246 ILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGG 305

Query: 305 DKTNPTALFFSLYLGKVDELVSILNTTFPVLGLAKKHCIETSWVESALIMGTGLQSGRPL 364
           +KT  T+ + +L+LG    L++++  +FP LGL  K CIE SW+ES   + +G  +  P 
Sbjct: 306 NKTVTTS-YNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYI-SGFPTHTPT 365

Query: 365 EDLL-SKASLTNGSTKIKSDYIKQPIPMVAIEGIWQRLKLEDIEMPQLLIAPYGGRMSQV 424
             LL  K+     S K KSD++K PIP   ++GI+++L  EDI  P ++  PYGG M+++
Sbjct: 366 NVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMMAKI 425

Query: 425 FESETPFPHRAGYLYKIGYVVNWEHEGKDVEKRHLRWIRELYSYMTPFVSKSPRAAYINY 484
            ES+ PFPHR G L+K+ YV +W    K    RH+ WIR+LYSYMTP+VS +PR AY+NY
Sbjct: 426 PESQIPFPHRKGVLFKVQYVTSWLDSDKR-PSRHINWIRDLYSYMTPYVSSNPREAYVNY 485

Query: 485 RDLDIGSNNRYGKTNYKEASVWGLKYFGNNFNRLVDVKTRVDPRNFFTHEQSIP 524
           RDLD+G N +  KT  K+A VWG  YF NNFNRL+ +K +VDP NFF HEQSIP
Sbjct: 486 RDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534