PI0024611 (gene) Melon (PI 482460) v1

Overview


Name	PI0024611
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Reverse transcriptase
Location	chr01: 19830824 .. 19831285 (+)
RNA-Seq Expression	PI0024611
Synteny	PI0024611

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTTCTCACTAAGTATCACGCGGTGTTGGATTGCTCCAATAAGGAGGTAGTACAAAGAGAACCAGGAAAATTTGAAGTTAAATTTATAGGCGACAAGAAAGTGGAATTAGCAAGTATGATATCAGTACTTAAGGCAAGGAAGTTGATTAAGAAGGGGCATACCGCCTACCTTGCTCGCATAGTTGACACCCAAGCGATAAGGAATAATCCAAGAAACATACCTATAGTTTGCGAATACTTGGATGTGTTTCCAAAAGAAATAAGTGGATTACCTCCAAAAAGAGAGGTTGAGTTTACAATTGAAGTTGTGCCTAGAACAAAACCAGTTTCTCAAACTCCTTACCGCATGGCACCAAGCGAGCTGAAAGAATTGAAAGATCAATTGGAGGAATTATTAGAGAAGGGTTATATTCGACCTAGTACCTCACCTTGGGGAGCCCTTGTTCTATTTGTGTAA

mRNA sequence

ATGGATTTTCTCACTAAGTATCACGCGGTGTTGGATTGCTCCAATAAGGAGGTAGTACAAAGAGAACCAGGAAAATTTGAAGTTAAATTTATAGGCGACAAGAAAGTGGAATTAGCAAGTATGATATCAGTACTTAAGGCAAGGAAGTTGATTAAGAAGGGGCATACCGCCTACCTTGCTCGCATAGTTGACACCCAAGCGATAAGGAATAATCCAAGAAACATACCTATAGTTTGCGAATACTTGGATGTGTTTCCAAAAGAAATAAGTGGATTACCTCCAAAAAGAGAGGTTGAGTTTACAATTGAAGTTGTGCCTAGAACAAAACCAGTTTCTCAAACTCCTTACCGCATGGCACCAAGCGAGCTGAAAGAATTGAAAGATCAATTGGAGGAATTATTAGAGAAGGGTTATATTCGACCTAGTACCTCACCTTGGGGAGCCCTTGTTCTATTTGTGTAA

Coding sequence (CDS)

ATGGATTTTCTCACTAAGTATCACGCGGTGTTGGATTGCTCCAATAAGGAGGTAGTACAAAGAGAACCAGGAAAATTTGAAGTTAAATTTATAGGCGACAAGAAAGTGGAATTAGCAAGTATGATATCAGTACTTAAGGCAAGGAAGTTGATTAAGAAGGGGCATACCGCCTACCTTGCTCGCATAGTTGACACCCAAGCGATAAGGAATAATCCAAGAAACATACCTATAGTTTGCGAATACTTGGATGTGTTTCCAAAAGAAATAAGTGGATTACCTCCAAAAAGAGAGGTTGAGTTTACAATTGAAGTTGTGCCTAGAACAAAACCAGTTTCTCAAACTCCTTACCGCATGGCACCAAGCGAGCTGAAAGAATTGAAAGATCAATTGGAGGAATTATTAGAGAAGGGTTATATTCGACCTAGTACCTCACCTTGGGGAGCCCTTGTTCTATTTGTGTAA

Protein sequence

MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLARIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAPSELKELKDQLEELLEKGYIRPSTSPWGALVLFV

Homology

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BLAST of PI0024611 vs. ExPASy TrEMBL
Match: A0A5D3BRB8 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001320 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 9.0e-49
Identity = 101/153 (66.01%), Postives = 123/153 (80.39%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           M FLTKYHA  DCSN EVV +E  KF+VK + +KKVELA++ISVLKA K I+K +  YL 
Sbjct: 33  MYFLTKYHAARDCSNNEVVLKELEKFKVKIVSNKKVELANIISVLKAMKFIRKRYIVYLP 92

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            IVDTQA+ N+P ++ IVC+YLDVF KE++GLP KRE++FTIEVV  T P+SQT Y M P
Sbjct: 93  YIVDTQAVNNDPISVLIVCKYLDVFSKEMNGLPLKREIKFTIEVVLETTPISQTSYSMEP 152

Query: 121 SELKELKDQLEELLEKGYIRPSTSPWGALVLFV 154
           SELKELKDQLEELLE+ YI+P+TSPWG  +LFV
Sbjct: 153 SELKELKDQLEELLERCYIQPNTSPWGTPILFV 185

[Close]

BLAST of PI0024611 vs. ExPASy TrEMBL
Match: A0A5D3D2P1 (Putative Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G00770 PE=4 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 5.9e-48
Identity = 97/152 (63.82%), Postives = 123/152 (80.92%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           ++FLTKYHA+ D SNKEVV R+P K E+KF  DK+VE   +I VLKARKL+K  H +YLA
Sbjct: 204 VNFLTKYHAIFDYSNKEVVLRDPKKIEIKFEADKRVE--RIIFVLKARKLMKNEHVSYLA 263

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            +++T A RN+P  +PIVCEY+DVFPKE+SGL P+RE++FTIE+V  T P+SQTPYRMA 
Sbjct: 264 HVINTLAPRNDPSKVPIVCEYMDVFPKELSGLLPQREIKFTIEIVQGTTPISQTPYRMAL 323

Query: 121 SELKELKDQLEELLEKGYIRPSTSPWGALVLF 153
            ELKELK+QL+EL+EK YI+P TSPW A +LF
Sbjct: 324 GELKELKNQLKELIEKDYIQPRTSPWRAPILF 353

[Close]

BLAST of PI0024611 vs. ExPASy TrEMBL
Match: A0A5A7V2B5 (Gag protease polyprotein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold749G001070 PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.4e-46
Identity = 93/129 (72.09%), Postives = 109/129 (84.50%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           MDFLTKYHA+L+CSNKEVV R+P KFE+KF+GDK V+LA + SVL ARKLIKKGHT YLA
Sbjct: 23  MDFLTKYHAILNCSNKEVVLRDPRKFEIKFVGDKMVKLAGITSVLMARKLIKKGHTPYLA 82

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            +VDTQ ++ +P  +PIVCEYLDVFP E++GLPPKRE+E  IEV PRT P+SQT YRM P
Sbjct: 83  CVVDTQPLQTDPSKVPIVCEYLDVFPGELNGLPPKREIELMIEVAPRTTPISQTLYRMVP 142

Query: 121 SELKELKDQ 130
           SELKELK Q
Sbjct: 143 SELKELKIQ 151

[Close]

BLAST of PI0024611 vs. ExPASy TrEMBL
Match: A0A5A7TLU6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold824G001060 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.9e-46
Identity = 97/153 (63.40%), Postives = 120/153 (78.43%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           MDFL  ++A +DC  KEVV R+PG  EV F G +K    S+ISVLKA KL++KG TA+LA
Sbjct: 488 MDFLFAHYASMDCHRKEVVFRKPGFAEVVFRGMRKAVSRSLISVLKAEKLLRKGCTAFLA 547

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            IV  Q  +  P ++P+V E+LDVFP ++SGLPP RE+EFTIE++P T P+SQ PYRMAP
Sbjct: 548 HIVVVQREKLKPEDVPVVKEFLDVFPDDLSGLPPDREIEFTIELLPGTAPISQAPYRMAP 607

Query: 121 SELKELKDQLEELLEKGYIRPSTSPWGALVLFV 154
           SELKELK QL+EL++KGYIRPS SPWGALVLFV
Sbjct: 608 SELKELKMQLQELVDKGYIRPSVSPWGALVLFV 640

[Close]

BLAST of PI0024611 vs. ExPASy TrEMBL
Match: A0A5A7TIG0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G00120 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 7.2e-46
Identity = 96/153 (62.75%), Postives = 119/153 (77.78%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           MDFL  ++A +DC  KEVV R+PG  EV F G +K    S+ISVLKA KL++KG TA+LA
Sbjct: 478 MDFLFAHYASMDCHRKEVVFRKPGFAEVVFRGMRKAVSRSLISVLKAEKLLRKGCTAFLA 537

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            IV  Q  +  P ++P+V E+LDVFP ++SGLPP RE+EFTIE++P T P+SQ PYRMAP
Sbjct: 538 HIVVVQREKLKPEDVPVVKEFLDVFPDDLSGLPPDREIEFTIELLPETAPISQAPYRMAP 597

Query: 121 SELKELKDQLEELLEKGYIRPSTSPWGALVLFV 154
           SELKELK QL+EL++KGYIRPS SPWGA VLFV
Sbjct: 598 SELKELKMQLQELVDKGYIRPSVSPWGAPVLFV 630

[Close]

BLAST of PI0024611 vs. NCBI nr
Match: TYK01558.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 203.0 bits (515), Expect = 1.9e-48
Identity = 101/153 (66.01%), Postives = 123/153 (80.39%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           M FLTKYHA  DCSN EVV +E  KF+VK + +KKVELA++ISVLKA K I+K +  YL 
Sbjct: 33  MYFLTKYHAARDCSNNEVVLKELEKFKVKIVSNKKVELANIISVLKAMKFIRKRYIVYLP 92

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            IVDTQA+ N+P ++ IVC+YLDVF KE++GLP KRE++FTIEVV  T P+SQT Y M P
Sbjct: 93  YIVDTQAVNNDPISVLIVCKYLDVFSKEMNGLPLKREIKFTIEVVLETTPISQTSYSMEP 152

Query: 121 SELKELKDQLEELLEKGYIRPSTSPWGALVLFV 154
           SELKELKDQLEELLE+ YI+P+TSPWG  +LFV
Sbjct: 153 SELKELKDQLEELLERCYIQPNTSPWGTPILFV 185

[Close]

BLAST of PI0024611 vs. NCBI nr
Match: KAA0031989.1 (putative Retrotransposon protein [Cucumis melo var. makuwa] >TYK16809.1 putative Retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 200.3 bits (508), Expect = 1.2e-47
Identity = 97/152 (63.82%), Postives = 123/152 (80.92%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           ++FLTKYHA+ D SNKEVV R+P K E+KF  DK+VE   +I VLKARKL+K  H +YLA
Sbjct: 204 VNFLTKYHAIFDYSNKEVVLRDPKKIEIKFEADKRVE--RIIFVLKARKLMKNEHVSYLA 263

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            +++T A RN+P  +PIVCEY+DVFPKE+SGL P+RE++FTIE+V  T P+SQTPYRMA 
Sbjct: 264 HVINTLAPRNDPSKVPIVCEYMDVFPKELSGLLPQREIKFTIEIVQGTTPISQTPYRMAL 323

Query: 121 SELKELKDQLEELLEKGYIRPSTSPWGALVLF 153
            ELKELK+QL+EL+EK YI+P TSPW A +LF
Sbjct: 324 GELKELKNQLKELIEKDYIQPRTSPWRAPILF 353

[Close]

BLAST of PI0024611 vs. NCBI nr
Match: KAA0061410.1 (Gag protease polyprotein-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 195.7 bits (496), Expect = 3.0e-46
Identity = 93/129 (72.09%), Postives = 109/129 (84.50%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           MDFLTKYHA+L+CSNKEVV R+P KFE+KF+GDK V+LA + SVL ARKLIKKGHT YLA
Sbjct: 23  MDFLTKYHAILNCSNKEVVLRDPRKFEIKFVGDKMVKLAGITSVLMARKLIKKGHTPYLA 82

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            +VDTQ ++ +P  +PIVCEYLDVFP E++GLPPKRE+E  IEV PRT P+SQT YRM P
Sbjct: 83  CVVDTQPLQTDPSKVPIVCEYLDVFPGELNGLPPKREIELMIEVAPRTTPISQTLYRMVP 142

Query: 121 SELKELKDQ 130
           SELKELK Q
Sbjct: 143 SELKELKIQ 151

[Close]

BLAST of PI0024611 vs. NCBI nr
Match: KAA0042309.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 195.3 bits (495), Expect = 3.9e-46
Identity = 97/153 (63.40%), Postives = 120/153 (78.43%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           MDFL  ++A +DC  KEVV R+PG  EV F G +K    S+ISVLKA KL++KG TA+LA
Sbjct: 488 MDFLFAHYASMDCHRKEVVFRKPGFAEVVFRGMRKAVSRSLISVLKAEKLLRKGCTAFLA 547

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            IV  Q  +  P ++P+V E+LDVFP ++SGLPP RE+EFTIE++P T P+SQ PYRMAP
Sbjct: 548 HIVVVQREKLKPEDVPVVKEFLDVFPDDLSGLPPDREIEFTIELLPGTAPISQAPYRMAP 607

Query: 121 SELKELKDQLEELLEKGYIRPSTSPWGALVLFV 154
           SELKELK QL+EL++KGYIRPS SPWGALVLFV
Sbjct: 608 SELKELKMQLQELVDKGYIRPSVSPWGALVLFV 640

[Close]

BLAST of PI0024611 vs. NCBI nr
Match: KAA0041109.1 (hypothetical protein E6C27_scaffold128G00120 [Cucumis melo var. makuwa])

HSP 1 Score: 193.4 bits (490), Expect = 1.5e-45
Identity = 96/153 (62.75%), Postives = 119/153 (77.78%), Query Frame = 0


Query: 1   MDFLTKYHAVLDCSNKEVVQREPGKFEVKFIGDKKVELASMISVLKARKLIKKGHTAYLA 60
           MDFL  ++A +DC  KEVV R+PG  EV F G +K    S+ISVLKA KL++KG TA+LA
Sbjct: 478 MDFLFAHYASMDCHRKEVVFRKPGFAEVVFRGMRKAVSRSLISVLKAEKLLRKGCTAFLA 537

Query: 61  RIVDTQAIRNNPRNIPIVCEYLDVFPKEISGLPPKREVEFTIEVVPRTKPVSQTPYRMAP 120
            IV  Q  +  P ++P+V E+LDVFP ++SGLPP RE+EFTIE++P T P+SQ PYRMAP
Sbjct: 538 HIVVVQREKLKPEDVPVVKEFLDVFPDDLSGLPPDREIEFTIELLPETAPISQAPYRMAP 597

Query: 121 SELKELKDQLEELLEKGYIRPSTSPWGALVLFV 154
           SELKELK QL+EL++KGYIRPS SPWGA VLFV
Sbjct: 598 SELKELKMQLQELVDKGYIRPSVSPWGAPVLFV 630

The following BLAST results are available for this feature:

BLAST of PI0024611 vs.


Match Name	E-value	Identity	Description

BLAST of PI0024611 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BRB8	9.0e-49	66.01	DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A5D3D2P1	5.9e-48	63.82	Putative Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7V2B5	1.4e-46	72.09	Gag protease polyprotein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A5A7TLU6	1.9e-46	63.40	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82...	[more]
A0A5A7TIG0	7.2e-46	62.75	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold12...	[more]

BLAST of PI0024611 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK01558.1	1.9e-48	66.01	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa]	[more]
KAA0031989.1	1.2e-47	63.82	putative Retrotransposon protein [Cucumis melo var. makuwa] >TYK16809.1 putative...	[more]
KAA0061410.1	3.0e-46	72.09	Gag protease polyprotein-like protein [Cucumis melo var. makuwa]	[more]
KAA0042309.1	3.9e-46	63.40	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0041109.1	1.5e-45	62.75	hypothetical protein E6C27_scaffold128G00120 [Cucumis melo var. makuwa]	[more]

BLAST of PI0024611 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 116..136
None	No IPR available	GENE3D	3.10.10.10	HIV Type 1 Reverse Transcriptase, subunit A, domain 1	coord: 89..153 e-value: 1.1E-16 score: 62.9
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 1..153
None	No IPR available	PANTHER	PTHR24559:SF330	RNA-DIRECTED DNA POLYMERASE HOMOLOG	coord: 1..153
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 77..153

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0024611.1	PI0024611.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
biological_process	GO:0006508	proteolysis
molecular_function	GO:0005488	binding
molecular_function	GO:0004518	nuclease activity
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0008233	peptidase activity