PI0023134 (gene) Melon (PI 482460) v1

Overview


Name	PI0023134
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	cytochrome P450 81D1-like
Location	chr11: 23650095 .. 23656172 (-)
RNA-Seq Expression	PI0023134
Synteny	PI0023134

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTTCTTTTGCTCTCTATTTCTCTAATCTCTCTAATCTCTCTTTTCCTTGCCTTCAACTTCCTTCATCATTCTCATCACAAAAACCTCCCACCATCTCCCTCTTTTTCTCTTCCATTAATAGGTCATCTCCATCTCATCAAACATCCTGTCCATCGAACCCTTCACAACCTCTCCCAAAAGCATGGCCACGTCCTCTCACTCCGACTTGGCTCACGTCTCGTCGTAGTTGTATCCTCTTCTTCTCTTGTCCAATAATGCTTTACAATAAACGATGTAGTTCTTGCTAATCGCCCATTGATGAAGAGTGCGAAGCACTTCACATACAACTATACCAACTTGGCCATCTCGCCCTACGGTGAACACTGGTGAAACCTCCGTCGAAGCAGCACTCTCGAGATCTTCTCCACAACTCGCCTTAATCTTTTCATGGGAACTAGAGAAGATGAAGTCAAACGCTTGTTGTGTAAACTGTGGGATAAAGATTCTTTTCAAGAGTTTAAGGTAGTGAAAATGTTTCCTATGTTTATGGATCCTATGTTGAATATTGTTATTAAAATGATTGGTGGAAAGAAATATTGTGGGAGGATTTTTGAAGATGAGGAAAAATCTAAGAAATTTAAAGAGATGGTGACAGCTTCTAGCAATTATTGGAATAGGAAATCCAGGAGATTTTATACCCTTATGGAATTGGATTGATCCAACTTGTTATGACAAGAAAGTAAAGAAGCTTTCGAAGACATCAGATGAATTTATTCAACAATTAATTGATGAGATTAGGAATCAGAGTGATGGAGAGAACACAATGATTCAACATTTACTTGGTTTTCAGAATTCCCAACCAGAATATTATAGTGACCAAATTATCAAAGGCCTCGTTCAAGTATGTTTTCTCTTTCTCTAATATAGCAATTTCTTCAACCGTTTTTATTGTTTCATATTTATGACAAATGAATGACAAGAGAGAGGGTGTCAAATCCACTCGAACTAGTTATATATTGAGTAGTATATAAGTGGTAATTAAGTTCAGGTCAATCCATAGGTGACGCAAGAAGATTAGTTTCTCAATATCCATTTTTAGTTAAAGCAGTAAATTGAACAGAGAATCAAAGGTGCATGAAAGTAAAGGCATGAAAGTGAAAGAATAGACATATAACCAATAAAAACGTTCTAGCGTAGAGCAACAGAACAACCCAATGTAATCTTGATCATTGAACGTCTCTCAAAATTTGATTTTAACATATAGTCTATGCCTAGCCCAGTTGATTAGAAACGCTAACTAACAGTTTTAATTACCCAATAGCAAATTTAATTACATGAAATGCATGCCAACAAATTGAGCTAAACTCCATTACGTTTTAGGGATTATTTTAAAATAAATTTTCAATATGAAACCCCTAATTAAAAATTTAATACGGGTTGATAAAATAACGCTCTTTGTTGTCTTTGAAAAAATTACATAACGTAGCCTATCTAACAATTGTTACTTAGGGATTAACATAAAATTATATGTCACATGTAATACGCTAATCCTTAGCTTTCGACTATACATCCTCAAGTAGTTTGTCAGATGTTCATAAGAAAATAATACAATCAATAATGCAAGTTGATCAAGATGAAAACCCAGACACAAAATTATAAATCAAACAAAATCCTCACAAATTAATTACAAATGGTTATTCCAAAATCCACTAAACCACAAAACACATAAAATTTACCTTACTATGGTGATGGAGAGAAGCTAAAATCTGACATCCTTTTGCTTGATTACAGAAATAAGACGAAAGAGAATCTAAAATCTAATTTTGTAAATAGATAGAGGTCGAAAAAATGAAAAAAAGGAGTGGTCATTCCCCCAAAATGCTCATCCCAACAAAAGTTTTTGTTAAACAAATAAATTTTTTAAAATTGTATTTGGTAATTTTATTTTTGTGTGGTGAAAAGATGAGAAGTATATGAGATAGGTGCATCCTATGATGTACACCCAAGTTTTTTTCTTGAACAAATGATTTACTATACCTATTAACTAAAAAAATATTGTGCAAAATTTATAGTTGTGTTTCAATTAAATTCATAAATTTGTTTAAGTGAATCAATTTAAATTGTTAATTAATATTGATATAGTTGCAAAAAATTTGTCCATGCATTCATTCTTACACGTGTGTACACAATTATACGTTTCACATCATATTTTCTCAACTAAATGTGAAAAAGAGTGTAAACTAATACAATTATGAAAGTTTAGAGTTTGATAAAAGTGTTGAAAATTAATTAAACATGTTTTAAGTAGGTAAGACCTCGTAGATTAATTAATTTATGAGAGTTGAGAAAGATAGAACGTTAGTTAATAAAGTATATTAATTGATGCGAGTTGGTAAAGTCATGTATTGGTAGAGAGTGTGAGCAATGTTAATAAATTATGTATAGGACTTTAAATGTTTTTATGGGGAGAGTAACTCAGATTTTCCATGACAATCTTATAAATAAGATTGATATTTTTGTATTGTAAAAGCAACCAAGTATATGAAGAAGAATACACATAAAGAGAGTCAATTTGAAGAGTCTTCTAAATTAGTGTTTTTGTTTTCTCTCAAATATTCTTTTGTCTTTGTGTGTCCATCACACACTTTCAACAAAGTGATATTAGAGTTTGAGTTTGGAGTTTGAAACAATTTGGTCCCTTGCAAGTGCATCAACTTATGAAAGAAAATTATACCAGTTGGTGTATTCATATGAAAGCTCTACTTGGTTTACAAGATATTCGAGACATTGTTAATATTAGTTATGAAGAGCTAACAAATGATGTGGCTTTGAGTCAAAGATCCAAGAGAAGCTTTGGAATATACAAGAAAGAAAGACTAAAAGGTTTTCACCATCATTCATCAAGCCATTGATGATCCAAATTTTGAGAAGATTTTTGGAGCAACTACTGCACATCAAGCATGGAAAAGTTTGGAGAATACATATAAAAGAGTAGATTGAGTGAAGAAGGTTCGACTTAGAAAATTGAGAGGTAATCACTACATAAGAAAGAGTCTAAATCAATTTTAGATTATAATCCAATTGTTACAAATAAGACAATGGTAAGCAGAGATGTTGTGTTTGATGAAGAAACATCATGGAATTAAAATGATAAATAAGAAGATTACAATGGTAAGCAGACATGTTGTGTTTAATGAAGATGCATCATGGAATTGGAATGATAAATTAAAAGACTACAAGTTTTTTTTTTTTTTTTTTTTATGATTATGATGAGTCTAGTGACATTGTTTTTTCAGCAACACCATCAATATCATCAATCTCCACAAAAAAAAAAAAAAAAAAAAAAAAAACACACCTTCATCATCTATAAATTTAAGTAAAGGGTCTCGTGGCATGAGAAGTTTACGAGACATATATGATGAAACTGAAGAGTTGAGTCAAAGTTTTAATAACTTGACTCTTTCTTGTCATGATTATCACATTGAAAACCTTTGAATTTTGAAAAAGCTTCACAAGATGAAAATGGAATGCTATAGATGAAGAGTTAAAATTCATAAAGAAGAATGATACATTGGAACATTCTACTCTTCCAGGTAGAAACAAGTAGTAGGTGTCAAATGAGTGTTCAAGACAAAAAAATTGAAAAGGGAGAAGTTGAGAGATATTAGACAAATTTTAGTTGTAAAATGCTATTCTCAAAGAAAAGTCGTTGATTACGAGAAGTATTTGCTTCCGTTACTCGTTTGAAGATCATCGAGTTGTTAACTGCCCTTGCTACTCAAAATATATGAAAGATCTTTCAGATGGATGTCAAATCCACATTCTTGAATGGATATCTATGGAAGAAATCTACTCAGAACAACCTCTTAGTTATGTTGTGAAAGGTCGAGAAAATAAAGTTCTAAAATTGAATAAGGCGTTGTACGAATTGAAACAAGCACCAATAATGTGGAATAGCAGAATCAACAAATATTTCCTTGGCAATGGGTATTTGAGATGCACTTATGAACATTCTTTTTATACTAAGATTAATGGTCGTGGAGATATTTTGTTGGTTTGTCTGTATGTGGATGACTTAATGTCTACAGAAAATTGTGCAAGTATGTTTAACGATCTCAAGAAGGCGATTACTCAAGAATTTGAAACGATAGATATTGGGTTCATCTCATATTATCTTGGCATATTGAGCTGTGAACGATATGCTAAAAAGGTTGAAGATGATCAATTCTAGACATGTCACAACTCTAGTAGAAATTGGGACAAAACTATCCAAACATGAAGAAGGAGATGTTATTGATCTTTCATATTGAAAAAGTTTGATTGAAACTTTGAGATATTTGACTTACACATGACCAAATACTCTTTTTAGCATTGGATTGGTAAGTCGATTTATACTATCTTCTACAACCACTTATTTGAAAGGCTAAGAGAATTCTTCGCTACTTCAAAAGTATGCTTGATTATGAGTTATTTTATTTATCATCTAAAGAACTCAAGCTTGAAGGTTATTGTGATAGGTTCAAGGTTGGAGGTGTTAATGATCGAAAAAGTACAAGTGGATATGTTTTCTTTGCTTATAATACTGCTTTTATTTGGAGTTCTAAGAAGTAACTTATTGTGACATTATCAATTTGTGAGGCAGAATATGTCGTAGCTTCATGTGTGTTTGTTATGTACTTTGGTTAAGGAAATTGCTGAAGACAGTTGCTATTTTGCAAGACAATGCAATAGTGATCCATGTAGACAATAAGTCAACAATTGCTCTAGTGAAGAGTCTAATGTTCGATGATCATAACAGACACATTAATACAATATTTCACTTTATCAAGATTGAATTTCAAAGGAGGTTAAAGTTGAATAAATGAAGAATTGACGATCAAATTGTGGATACTTTTATATATGAATCCACTCAAAGTTGATGTGTTTAATAAGTTAAGAACTTTGTTTGGAGTTCTTTAGAAAACATGTTTTAAGAAGGGATGTTGAAAATTAATTAAACATGTTTTAAGTAGATAAGTCGTTATAGATGAATTAATTTATGAGAGTGGACAAAGGTAAAGAATGTGAGTTAATTATGTATATTAATTGATAAGAGTTGATAACGTTGTGTACTAGAGAGAGTTAACTACGTTAATAAATTAAATGTAGAACTTTAAATGTGTTTATGGTGAGAGTTATAGATTTAGATTTTTCATGACAATCCTCTGAATAGGACTGATGCTTTTGTATTCTAAAAGTAACCAAGTGGGAAGAAGAGTACACACAAAAAGAGAGTTAATTTGAAGAGTCTTCTAGATAAGTGTTTTTGTTTTATCTCAAATATTCTTTTGTTGATTTTTTATTTGGAAGTGTCCATTACACACTTCCAACAAAAAGCTCTAAATTTAGGAATAATATAAATTGATATATTTCTATTTTATGTGATCATTATCCTTTTCGATTTTTAATCTTTTTGTGATTTATTCTTTCAGGTAGTACTATTAGCAGGAACTGATGCAGTAGCAGCGACCTTAGAATGGGCACTATCTCACCTACTCAATAATCCAGAGGTGTTAAAGAAAGCTAGAGATGAGATAGATGGTTCCATCGGACAAGAGCGTCTTGTTAATGAAACTGATTTGTCAAGTTTGAGTTATTTGCAAGGAGTAATTTATGAGACCTTACACTTGAACCCAACAGCTCCTCTACTTGCACCACATTGTGTATCTGAAGACTGCAAAATAGGAGGGTACGATATACCGCGTGACACAATGGTATTGATTAATGCTTGGGCCATACATAGAGACCCAAGCTTATGGGAAGATGCCACAAGTTTCAAGCCGGAAAGACATGGAAATGCAAATGGAGTTGATGCACACAAATTATTACCATTTGGGTTGGGAAGGAGAGCTTGCCCTGGAATGGGAATGGCAACATCGTGTAATTGCCTTGGCTTTGGCATCATTGATTCAATGCTTTGAGTGGCAAAGACTCAGCAACTCATTGGTGGATATGGCAGAAGGTGAAGGAGTCACTATGCCTAAAGCTCAACCATTGACAGCCAAGTGTAGACCACGTCCAATCATGAAGAAAATGTTGTCAATGAATCAATAA

mRNA sequence

ATGGATCTTCTTTTGCTCTCTATTTCTCTAATCTCTCTAATCTCTCTTTTCCTTGCCTTCAACTTCCTTCATCATTCTCATCACAAAAACCTCCCACCATCTCCCTCTTTTTCTCTTCCATTAATAGGTCATCTCCATCTCATCAAACATCCTGTCCATCGAACCCTTCACAACCTCTCCCAAAAGCATGGCCACGTCCTCTCACTCCGACTTGGCTCACGTCTCGTCGTAGTTGTATCCTCTTCTTCTCTTATCTTCTCCACAACTCGCCTTAATCTTTTCATGGGAACTAGAGAAGATGAAGTCAAACGCTTGTTGTGTAAACTGTGGGATAAAGATTCTTTTCAAGAGTTTAAGGTAGTGAAAATGTTTCCTATGTTTATGGATCCTATGTTGAATATTGTTATTAAAATGATTGGTGGAAAGAAATATTCAATTATTGGAATAGGAAATCCAGGAGATTTTATACCCTTATGGAATTGGATTGATCCAACTTGTTATGACAAGAAAGTAAAGAAGCTTTCGAAGACATCAGATGAATTTATTCAACAATTAATTGATGAGATTAGGAATCAGAGTGATGGAGAGAACACAATGATTCAACATTTACTTGGTTTTCAGAATTCCCAACCAGAATATTATAGTGACCAAATTATCAAAGGCCTCGTTCAAGTAGTACTATTAGCAGGAACTGATGCAGTAGCAGCGACCTTAGAATGGGCACTATCTCACCTACTCAATAATCCAGAGGTGTTAAAGAAAGCTAGAGATGAGATAGATGGTTCCATCGGACAAGAGCGTCTTGTTAATGAAACTGATTTGTCAAGTTTGAGTTATTTGCAAGGAGTAATTTATGAGACCTTACACTTGAACCCAACAGCTCCTCTACTTGCACCACATTGTGTATCTGAAGACTGCAAAATAGGAGGGTACGATATACCGCGTGACACAATGGTATTGATTAATGCTTGGGCCATACATAGAGACCCAAGCTTATGGGAAGATGCCACAAGTTTCAAGCCGGAAAGACATGGAAATGCAAATGGAGTTGATGCACACAAATTATTACCATTTGGGTTGGGAAGGAGAGCTTGCCCTGGAATGGGAATGGCAACATCACTCAGCAACTCATTGGTGGATATGGCAGAAGGTGAAGGAGTCACTATGCCTAAAGCTCAACCATTGACAGCCAAGTGTAGACCACGTCCAATCATGAAGAAAATGTTGTCAATGAATCAATAA

Coding sequence (CDS)

ATGGATCTTCTTTTGCTCTCTATTTCTCTAATCTCTCTAATCTCTCTTTTCCTTGCCTTCAACTTCCTTCATCATTCTCATCACAAAAACCTCCCACCATCTCCCTCTTTTTCTCTTCCATTAATAGGTCATCTCCATCTCATCAAACATCCTGTCCATCGAACCCTTCACAACCTCTCCCAAAAGCATGGCCACGTCCTCTCACTCCGACTTGGCTCACGTCTCGTCGTAGTTGTATCCTCTTCTTCTCTTATCTTCTCCACAACTCGCCTTAATCTTTTCATGGGAACTAGAGAAGATGAAGTCAAACGCTTGTTGTGTAAACTGTGGGATAAAGATTCTTTTCAAGAGTTTAAGGTAGTGAAAATGTTTCCTATGTTTATGGATCCTATGTTGAATATTGTTATTAAAATGATTGGTGGAAAGAAATATTCAATTATTGGAATAGGAAATCCAGGAGATTTTATACCCTTATGGAATTGGATTGATCCAACTTGTTATGACAAGAAAGTAAAGAAGCTTTCGAAGACATCAGATGAATTTATTCAACAATTAATTGATGAGATTAGGAATCAGAGTGATGGAGAGAACACAATGATTCAACATTTACTTGGTTTTCAGAATTCCCAACCAGAATATTATAGTGACCAAATTATCAAAGGCCTCGTTCAAGTAGTACTATTAGCAGGAACTGATGCAGTAGCAGCGACCTTAGAATGGGCACTATCTCACCTACTCAATAATCCAGAGGTGTTAAAGAAAGCTAGAGATGAGATAGATGGTTCCATCGGACAAGAGCGTCTTGTTAATGAAACTGATTTGTCAAGTTTGAGTTATTTGCAAGGAGTAATTTATGAGACCTTACACTTGAACCCAACAGCTCCTCTACTTGCACCACATTGTGTATCTGAAGACTGCAAAATAGGAGGGTACGATATACCGCGTGACACAATGGTATTGATTAATGCTTGGGCCATACATAGAGACCCAAGCTTATGGGAAGATGCCACAAGTTTCAAGCCGGAAAGACATGGAAATGCAAATGGAGTTGATGCACACAAATTATTACCATTTGGGTTGGGAAGGAGAGCTTGCCCTGGAATGGGAATGGCAACATCACTCAGCAACTCATTGGTGGATATGGCAGAAGGTGAAGGAGTCACTATGCCTAAAGCTCAACCATTGACAGCCAAGTGTAGACCACGTCCAATCATGAAGAAAATGTTGTCAATGAATCAATAA

Protein sequence

MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQKHGHVLSLRLGSRLVVVVSSSSLIFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDPMLNIVIKMIGGKKYSIIGIGNPGDFIPLWNWIDPTCYDKKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLAGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLHLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANGVDAHKLLPFGLGRRACPGMGMATSLSNSLVDMAEGEGVTMPKAQPLTAKCRPRPIMKKMLSMNQ

Homology

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BLAST of PI0023134 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.1e-101
Identity = 212/495 (42.83%), Postives = 285/495 (57.58%), Query Frame = 0


Query: 4   LLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQKH 63
           LL +   + L+S+ L   F      +NLPPSP  +LP+IGHLHLI   +HR+L++LS+K+
Sbjct: 6   LLYTFLAVVLLSISLKL-FPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 65

Query: 64  GHVLSLRLGSRLVVVVSSSSL--------------------------------------- 123
           G V SL+LG+RLV+VVSS +                                        
Sbjct: 66  GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 125

Query: 124 -----------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDPMLN 183
                      IFS   LN F+  REDEVK+LL  L+ + S Q+F  V+M     +   N
Sbjct: 126 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLY-QSSGQDFGKVEMKSKLSELSFN 185

Query: 184 IVIKMIGGKKY---------------------SIIGIGNPGDFIPLWNWIDPTCYDKKVK 243
           + ++M+ GK+Y                        G  NPGDF+P   WID   Y+KKV 
Sbjct: 186 VTMRMVAGKRYFGQDVDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVS 245

Query: 244 KLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLAGTD 303
           K+S+  D F+Q+LI E R   +   TMI HLL  Q SQPEYY+DQIIKG++ V+LLAGTD
Sbjct: 246 KISQEMDAFLQRLIHESRINKNNV-TMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTD 305

Query: 304 AVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLHLNP 363
             A T+EWA+S LLN+PE L+KAR EI+  +G  RL+ E DL  L+YL  +I ET  L P
Sbjct: 306 TSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCP 365

Query: 364 TAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANGVDA 411
            AP+L PH  S+DCK+ GYD+P+ T++L+NAWAIHRDP  W++ T FKPERHG    ++ 
Sbjct: 366 AAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERHGGVE-LEP 425

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BLAST of PI0023134 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.0e-96
Identity = 211/497 (42.45%), Postives = 281/497 (56.54%), Query Frame = 0


Query: 9   SLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQKHGH--- 68
           S+ SLI L ++F FL     +NLPPSP   LP+IGHL L+K P+HRTL + S+   H   
Sbjct: 12  SIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDG 71

Query: 69  --VLSLRLGSRLVVVVSSSSL--------------------------------------- 128
             V+SLRLGSRLV VVSS  +                                       
Sbjct: 72  GGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGD 131

Query: 129 ------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDPML 188
                       IFST RLN F+  R DEV+RL+ +L  + +  +  VV++ PM MD   
Sbjct: 132 HWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL-SRLAGTKKTVVELKPMLMDLTF 191

Query: 189 NIVIKMIGGKKY----------------------SIIGIGNPGDFIPLWNWIDPTCYDKK 248
           N +++M+ GK+Y                      +    GN  D++P+      + Y+ +
Sbjct: 192 NNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENR 251

Query: 249 VKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLAG 308
           VKKL + +D+F+Q LID+ R Q +   TMI HLL  Q S  EYY+DQIIKG++ ++++AG
Sbjct: 252 VKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAG 311

Query: 309 TDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLHL 368
           T+  A TLEWALS+LLN+P+V+ KARDEID  +G +RL+ E DLS L YL+ ++ ETL L
Sbjct: 312 TNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRL 371

Query: 369 NPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANGV 411
           +P  PLL PH  SEDCKIG YD+PR T +L+NAWAIHRDP+ W+D  SFKPER       
Sbjct: 372 HPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEE-- 431

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BLAST of PI0023134 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 350.9 bits (899), Expect = 2.0e-95
Identity = 206/500 (41.20%), Postives = 283/500 (56.60%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSH-HKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNL 60
           M++L L    +  ++LF  FN +  +   KNLPP P  SLP+IG+LH +K P+HRT   L
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGL 60

Query: 61  SQKHGHVLSLRLGSRLVVVVSSSSL----------------------------------- 120
           S+K+GHV SL  GSRLVVVVSS+S                                    
Sbjct: 61  SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120

Query: 121 ---------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMD 180
                          + S  R+N F G R DE +RL+ +L D DS   F  +++     D
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLAD-DSSTNFAEMELSSRLYD 180

Query: 181 PMLNIVIKMIGGKKY------------------------SIIGIGNPGDFIPLWNWIDPT 240
              N +++MI GK+Y                         + G  N  DF+PL  ++D  
Sbjct: 181 MTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFE 240

Query: 241 CYDKKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQV 300
             +K++K +S  +D F++ LI+E R + +  NTMI HLL  Q+SQPEYY+DQIIKGL   
Sbjct: 241 NLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALA 300

Query: 301 VLLAGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIY 360
           +LLAGTD+ A TLEW++S+LLN+PEVLKK +DE+D  +GQ+RLV+E+DL  L+YL+ VI 
Sbjct: 301 MLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVIN 360

Query: 361 ETLHLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHG 409
           ETL L   APLL PH  S++C IGGY +P+DT+VLINAWAIHRDP LW +AT+FKPER  
Sbjct: 361 ETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE 420

[Close]

BLAST of PI0023134 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 3.5e-92
Identity = 198/487 (40.66%), Postives = 274/487 (56.26%), Query Frame = 0


Query: 14  ISLFLAFNFLHHSH-HKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQKHGHVLSLRLG 73
           +S+F     L  S   KNLPP P+ SLP+IG+LH +K P++RT   L++K+G+V+SL  G
Sbjct: 14  LSIFFIIRLLFQSRKFKNLPPGPT-SLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWFG 73

Query: 74  SRLVVVVSSSSL------------------------------------------------ 133
           SRLVVVVSS S                                                 
Sbjct: 74  SRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITS 133

Query: 134 --IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDPMLNIVIKMIGGK 193
             + S  R+N F   R DE +RL+ KL  +DS  +F  V++   F D   N +++MI GK
Sbjct: 134 LDVLSNHRINNFAPIRRDETQRLIKKL-AEDSSTKFAEVELTFRFFDMTFNNIMRMISGK 193

Query: 194 KY------------------------SIIGIGNPGDFIPLWNWIDPTCYDKKVKKLSKTS 253
           +Y                         + G  N  DF+PL  ++D    +K+VK++ + +
Sbjct: 194 RYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIGEKN 253

Query: 254 DEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLAGTDAVAATL 313
           D F+  L+ E R++ +  NTMI HLL  Q SQPEYY+D IIKGL   +LLAGTD+ A TL
Sbjct: 254 DVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAGTDSSAVTL 313

Query: 314 EWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLHLNPTAPLLA 373
           EW +S++LN PEVLKK RDE+D  +GQ+RLV+E+DL  L+YL+ VIYETL L   APLL 
Sbjct: 314 EWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLYTPAPLLL 373

Query: 374 PHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANGVDAHKLLPF 409
           PH  +++C +GGY +PRDT+VLINAWAIHRDP  W +AT+FKPER       +  K++ F
Sbjct: 374 PHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKG--ELEKMIAF 433

[Close]

BLAST of PI0023134 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 3.5e-92
Identity = 204/494 (41.30%), Postives = 271/494 (54.86%), Query Frame = 0


Query: 4   LLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQKH 63
           L L ISL  LI     F F      KNLPP P   LP+IG+LH +K P+H T H LSQK+
Sbjct: 6   LSLIISLFFLIITLKVF-FNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTLSQKY 65

Query: 64  GHVLSLRLGSRLVVVVSSSSL--------------------------------------- 123
           G + SL  GSRLVVVVSS ++                                       
Sbjct: 66  GQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDH 125

Query: 124 -----------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDPMLN 183
                      I S+ RLN F+  R DE+ RL+ KL  K S+  F  V++ PMF +   N
Sbjct: 126 WRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQK-SYNGFTEVELRPMFSEMTFN 185

Query: 184 IVIKMIGGKKY------------------------SIIGIGNPGDFIPLWNWIDPTCYDK 243
            +++M+ GK+Y                        S+ G  N GDF+    W D    +K
Sbjct: 186 TIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEK 245

Query: 244 KVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLA 303
           ++KK+SK +D F+Q LIDE R      NTMI HLL  Q SQPEYY+DQIIKGL+ V+LLA
Sbjct: 246 RLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLA 305

Query: 304 GTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLH 363
           GTD  + T+EWA+S+LLN+PE++KKA++E+D  IG +R V+E D+S L YLQ ++YETL 
Sbjct: 306 GTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLR 365

Query: 364 LNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANG 407
           L+  APLL PH  SED  +GGY+IP++T++++NAW IHRDP+LW D T FKPER      
Sbjct: 366 LHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEG- 425

[Close]

BLAST of PI0023134 vs. ExPASy TrEMBL
Match: A0A5D3BF28 (Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00680 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.3e-178
Identity = 341/503 (67.79%), Postives = 363/503 (72.17%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MDLLLLSIS+ISL  L LAFNFLH  HHKNLPPSPSFSLPLIGHLHL+KHPVHRTL NLS
Sbjct: 1   MDLLLLSISIISLFCLLLAFNFLHQPHHKNLPPSPSFSLPLIGHLHLLKHPVHRTLQNLS 60

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
           QKHG VLSLR GS L VVVSSSSL                                    
Sbjct: 61  QKHGPVLSLRFGSCLAVVVSSSSLVQECFTKNDVVLANRPLLKSAKHLTYNYTNLAISPY 120

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVV-KMFPMFMD 180
                         I S +RLNLFMG REDEVKRLLCKLWD  SF+EFKVV +M PMFMD
Sbjct: 121 GEHWRNLRRISTLEILSLSRLNLFMGVREDEVKRLLCKLWDGYSFEEFKVVDQMLPMFMD 180

Query: 181 PMLNIVIKMIGGKKY----------------------SIIGIGNPGDFIPLWNWIDPTCY 240
            M NIV++M+ GKKY                      +I G GNPGDFIPLWNWIDPT Y
Sbjct: 181 LMFNIVMRMMCGKKYCGDDLKDEERSKKFKEMVKQVLAISGAGNPGDFIPLWNWIDPTRY 240

Query: 241 DKKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVL 300
           +K+VKKL+KTSDE IQQLIDEIRNQ+DGENTMIQHLLG QN+QPE YSDQIIKGL+QV+L
Sbjct: 241 EKRVKKLTKTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQVIL 300

Query: 301 LAGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYET 360
           L G D  A TLEWALSHLLNNPEVLKKA DEID SIGQE LV E D  SLSYLQG+I ET
Sbjct: 301 LGGIDTAAVTLEWALSHLLNNPEVLKKAIDEIDSSIGQECLVKEVDSFSLSYLQGIISET 360

Query: 361 LHLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNA 414
           L LNP APLL PHC  EDCKIGGYD+PRDT+VLINAW IHRDPSLWEDATSFKPERH NA
Sbjct: 361 LRLNPAAPLLVPHCAFEDCKIGGYDVPRDTIVLINAWTIHRDPSLWEDATSFKPERHENA 420

[Close]

BLAST of PI0023134 vs. ExPASy TrEMBL
Match: A0A1S3C2H7 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495922 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.3e-178
Identity = 341/503 (67.79%), Postives = 363/503 (72.17%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MDLLLLSIS+ISL  L LAFNFLH  HHKNLPPSPSFSLPLIGHLHL+KHPVHRTL NLS
Sbjct: 1   MDLLLLSISIISLFCLLLAFNFLHQPHHKNLPPSPSFSLPLIGHLHLLKHPVHRTLQNLS 60

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
           QKHG VLSLR GS L VVVSSSSL                                    
Sbjct: 61  QKHGPVLSLRFGSCLAVVVSSSSLVQECFTKNDVVLANRPLLKSAKHLTYNYTNLAISPY 120

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVV-KMFPMFMD 180
                         I S +RLNLFMG REDEVKRLLCKLWD  SF+EFKVV +M PMFMD
Sbjct: 121 GEHWRNLRRISTLEILSLSRLNLFMGVREDEVKRLLCKLWDGYSFEEFKVVDQMLPMFMD 180

Query: 181 PMLNIVIKMIGGKKY----------------------SIIGIGNPGDFIPLWNWIDPTCY 240
            M NIV++M+ GKKY                      +I G GNPGDFIPLWNWIDPT Y
Sbjct: 181 LMFNIVMRMMCGKKYCGDDLKDEERSKKFKEMVKQVLAISGAGNPGDFIPLWNWIDPTRY 240

Query: 241 DKKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVL 300
           +K+VKKL+KTSDE IQQLIDEIRNQ+DGENTMIQHLLG QN+QPE YSDQIIKGL+QV+L
Sbjct: 241 EKRVKKLTKTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQVIL 300

Query: 301 LAGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYET 360
           L G D  A TLEWALSHLLNNPEVLKKA DEID SIGQE LV E D  SLSYLQG+I ET
Sbjct: 301 LGGIDTAAVTLEWALSHLLNNPEVLKKAIDEIDSSIGQECLVKEVDSFSLSYLQGIISET 360

Query: 361 LHLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNA 414
           L LNP APLL PHC  EDCKIGGYD+PRDT+VLINAW IHRDPSLWEDATSFKPERH NA
Sbjct: 361 LRLNPAAPLLVPHCAFEDCKIGGYDVPRDTIVLINAWTIHRDPSLWEDATSFKPERHENA 420

[Close]

BLAST of PI0023134 vs. ExPASy TrEMBL
Match: A0A5A7SSK3 (Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00430 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.8e-177
Identity = 340/503 (67.59%), Postives = 362/503 (71.97%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MDLLLLSIS+ISL  L LAFNFLH  HHKN PPSPSFSLPLIGHLHL+KHPVHRTL NLS
Sbjct: 1   MDLLLLSISIISLFCLLLAFNFLHQPHHKNHPPSPSFSLPLIGHLHLLKHPVHRTLQNLS 60

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
           QKHG VLSLR GS L VVVSSSSL                                    
Sbjct: 61  QKHGPVLSLRFGSCLAVVVSSSSLVQECFTKNDVVLANRPLLKSAKHLTYNYTNLAISPY 120

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVV-KMFPMFMD 180
                         I S +RLNLFMG REDEVKRLLCKLWD  SF+EFKVV +M PMFMD
Sbjct: 121 GEHWRNLRRISTLEILSLSRLNLFMGVREDEVKRLLCKLWDGYSFEEFKVVDQMLPMFMD 180

Query: 181 PMLNIVIKMIGGKKY----------------------SIIGIGNPGDFIPLWNWIDPTCY 240
            M NIV++M+ GKKY                      +I G GNPGDFIPLWNWIDPT Y
Sbjct: 181 LMFNIVMRMMCGKKYCGDDLKDEERSKKFKEMVKQVLAISGAGNPGDFIPLWNWIDPTRY 240

Query: 241 DKKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVL 300
           +K+VKKL+KTSDE IQQLIDEIRNQ+DGENTMIQHLLG QN+QPE YSDQIIKGL+QV+L
Sbjct: 241 EKRVKKLTKTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQVIL 300

Query: 301 LAGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYET 360
           L G D  A TLEWALSHLLNNPEVLKKA DEID SIGQE LV E D  SLSYLQG+I ET
Sbjct: 301 LGGIDTAAVTLEWALSHLLNNPEVLKKAIDEIDSSIGQECLVKEVDSFSLSYLQGIISET 360

Query: 361 LHLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNA 414
           L LNP APLL PHC  EDCKIGGYD+PRDT+VLINAW IHRDPSLWEDATSFKPERH NA
Sbjct: 361 LRLNPAAPLLVPHCAFEDCKIGGYDVPRDTIVLINAWTIHRDPSLWEDATSFKPERHENA 420

[Close]

BLAST of PI0023134 vs. ExPASy TrEMBL
Match: A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.7e-150
Identity = 285/494 (57.69%), Postives = 341/494 (69.03%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MD+L LSISL SL SL LAF+FL  S  +NLPPSP FSLP++GHLHL+KHPVH+T H LS
Sbjct: 1   MDILFLSISL-SLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELS 60

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
            K+GHV SLR GSR+VVVVSS S                                     
Sbjct: 61  HKYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPY 120

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDP 180
                         IFSTTRLN+F+G REDEV+ L+ KL + +SF+EF  V++ PM  D 
Sbjct: 121 GEHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCE-NSFEEFTAVELEPMLFDL 180

Query: 181 MLNIVIKMIGGKKY-----------------SIIGIGNPGDFIPLWNWIDPTCYDKKVKK 240
             NIV++M+ GKKY                 + +G  NPGDFIP+WNWIDPT ++K++ K
Sbjct: 181 TFNIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIK 240

Query: 241 LSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLAGTDA 300
           L + SDE +Q+LIDEIRNQ  G+NTMI HLL  Q ++PEYYSDQIIKGL+QV+LLAG D 
Sbjct: 241 LGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDT 300

Query: 301 VAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLHLNPT 360
            A TLEWALS LLNNPEVL+KA+ EID  +GQERLVNE DLSSL YLQG+I+ETL LNP 
Sbjct: 301 AAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPA 360

Query: 361 APLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANGVDAH 411
           APLL PHC S DCKIGGYD+PRDT++LIN WAIHRDP+LWEDAT+FKPERHGN  GVD++
Sbjct: 361 APLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSY 420

[Close]

BLAST of PI0023134 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 7.1e-149
Identity = 294/501 (58.68%), Postives = 340/501 (67.86%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MD+LLLSISL SL SLF+ F+FL   + +NLPPSP F LPLIGHLHL+KHPVHRTLH LS
Sbjct: 81  MDILLLSISL-SLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLS 140

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
             +GHV SLR GSRLVVVVSS S                                     
Sbjct: 141 HNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPY 200

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDP 180
                         IFST+RLNLF+G REDEVK LL KL   DS QEF+ V++  M +D 
Sbjct: 201 GEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKL-RGDSLQEFRAVELDSMLLDH 260

Query: 181 MLNIVIKMIGGKKY-------------------SII---GIGNPGDFIPLWNWIDPTCYD 240
             NIV++MI GKKY                    I+   G  N GDF+PLWNWIDPT Y+
Sbjct: 261 TFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYE 320

Query: 241 KKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLL 300
           K++ K+ + SDE +Q+L+DEIRNQ DG NTMI HLL  QN+QPEY+SDQIIKGL Q++LL
Sbjct: 321 KRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLL 380

Query: 301 AGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETL 360
           AG D  A TLEWALS LLNNPEVLKKAR EID  IG++RLV+E DLSSLSYLQGVI ETL
Sbjct: 381 AGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETL 440

Query: 361 HLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNA- 412
            LNP APLL PHC SEDCKI GYDIPRDT++LINAWAIHRD ++WEDATSFKPERH N  
Sbjct: 441 RLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTM 500

[Close]

BLAST of PI0023134 vs. NCBI nr
Match: XP_004151923.1 (cytochrome P450 81Q32 [Cucumis sativus] >KAE8652307.1 hypothetical protein Csa_022053 [Cucumis sativus])

HSP 1 Score: 640.2 bits (1650), Expect = 1.2e-179
Identity = 340/502 (67.73%), Postives = 365/502 (72.71%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MDLLLLSISLISL SL LAFNFLH   HKNLPPSPSFSLPLIGHLHL++HPVHRTLHNLS
Sbjct: 1   MDLLLLSISLISLFSLLLAFNFLHQPRHKNLPPSPSFSLPLIGHLHLLRHPVHRTLHNLS 60

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
           +K G VLSLR GS L VVVSSSSL                                    
Sbjct: 61  EKLGPVLSLRFGSCLAVVVSSSSLVQECFTKNDIILANRPLVKSGKYLSYNYTNLVISPY 120

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDP 180
                         IFS +RLNLF+G REDEVKRLLCKLWD  SF+EFKVV+MFPMFMD 
Sbjct: 121 GEHWRNLRRISTLEIFSISRLNLFVGGREDEVKRLLCKLWDGYSFEEFKVVEMFPMFMDL 180

Query: 181 MLNIVIKMIGGKK----------------------YSIIGIGNPGDFIPLWNWIDPTCYD 240
           M NI+++M+ GKK                       +I G GNPGDFIPLWNWIDPTCY+
Sbjct: 181 MFNILMRMMSGKKNCGDDLKDEERSKKFKEMVKQVLAIAGAGNPGDFIPLWNWIDPTCYN 240

Query: 241 KKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLL 300
           K+VKKLSKTSDE IQQLIDEIRNQ+DGENTMIQHLLG QN+QPE YSDQIIKGL+QV+LL
Sbjct: 241 KRVKKLSKTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQVILL 300

Query: 301 AGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETL 360
           AG D  A TLEWAL HLLNNPEVLKKA DEID SIGQE LV E DL  LSYLQG+I+ETL
Sbjct: 301 AGIDTAAVTLEWALCHLLNNPEVLKKATDEIDSSIGQECLVKEVDLLRLSYLQGIIFETL 360

Query: 361 HLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNAN 414
            LNP  PLL PHC SEDCKIGGYD+PRDT VLINAWAIHRDPSLWEDATSFKPERH NAN
Sbjct: 361 RLNPATPLLVPHCASEDCKIGGYDVPRDTTVLINAWAIHRDPSLWEDATSFKPERHENAN 420

[Close]

BLAST of PI0023134 vs. NCBI nr
Match: XP_008455823.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo] >TYJ98342.1 cytochrome P450 81D1-like [Cucumis melo var. makuwa])

HSP 1 Score: 634.0 bits (1634), Expect = 8.9e-178
Identity = 341/503 (67.79%), Postives = 363/503 (72.17%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MDLLLLSIS+ISL  L LAFNFLH  HHKNLPPSPSFSLPLIGHLHL+KHPVHRTL NLS
Sbjct: 1   MDLLLLSISIISLFCLLLAFNFLHQPHHKNLPPSPSFSLPLIGHLHLLKHPVHRTLQNLS 60

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
           QKHG VLSLR GS L VVVSSSSL                                    
Sbjct: 61  QKHGPVLSLRFGSCLAVVVSSSSLVQECFTKNDVVLANRPLLKSAKHLTYNYTNLAISPY 120

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVV-KMFPMFMD 180
                         I S +RLNLFMG REDEVKRLLCKLWD  SF+EFKVV +M PMFMD
Sbjct: 121 GEHWRNLRRISTLEILSLSRLNLFMGVREDEVKRLLCKLWDGYSFEEFKVVDQMLPMFMD 180

Query: 181 PMLNIVIKMIGGKKY----------------------SIIGIGNPGDFIPLWNWIDPTCY 240
            M NIV++M+ GKKY                      +I G GNPGDFIPLWNWIDPT Y
Sbjct: 181 LMFNIVMRMMCGKKYCGDDLKDEERSKKFKEMVKQVLAISGAGNPGDFIPLWNWIDPTRY 240

Query: 241 DKKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVL 300
           +K+VKKL+KTSDE IQQLIDEIRNQ+DGENTMIQHLLG QN+QPE YSDQIIKGL+QV+L
Sbjct: 241 EKRVKKLTKTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQVIL 300

Query: 301 LAGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYET 360
           L G D  A TLEWALSHLLNNPEVLKKA DEID SIGQE LV E D  SLSYLQG+I ET
Sbjct: 301 LGGIDTAAVTLEWALSHLLNNPEVLKKAIDEIDSSIGQECLVKEVDSFSLSYLQGIISET 360

Query: 361 LHLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNA 414
           L LNP APLL PHC  EDCKIGGYD+PRDT+VLINAW IHRDPSLWEDATSFKPERH NA
Sbjct: 361 LRLNPAAPLLVPHCAFEDCKIGGYDVPRDTIVLINAWTIHRDPSLWEDATSFKPERHENA 420

[Close]

BLAST of PI0023134 vs. NCBI nr
Match: KAA0032531.1 (cytochrome P450 81D1-like [Cucumis melo var. makuwa])

HSP 1 Score: 631.3 bits (1627), Expect = 5.7e-177
Identity = 340/503 (67.59%), Postives = 362/503 (71.97%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MDLLLLSIS+ISL  L LAFNFLH  HHKN PPSPSFSLPLIGHLHL+KHPVHRTL NLS
Sbjct: 1   MDLLLLSISIISLFCLLLAFNFLHQPHHKNHPPSPSFSLPLIGHLHLLKHPVHRTLQNLS 60

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
           QKHG VLSLR GS L VVVSSSSL                                    
Sbjct: 61  QKHGPVLSLRFGSCLAVVVSSSSLVQECFTKNDVVLANRPLLKSAKHLTYNYTNLAISPY 120

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVV-KMFPMFMD 180
                         I S +RLNLFMG REDEVKRLLCKLWD  SF+EFKVV +M PMFMD
Sbjct: 121 GEHWRNLRRISTLEILSLSRLNLFMGVREDEVKRLLCKLWDGYSFEEFKVVDQMLPMFMD 180

Query: 181 PMLNIVIKMIGGKKY----------------------SIIGIGNPGDFIPLWNWIDPTCY 240
            M NIV++M+ GKKY                      +I G GNPGDFIPLWNWIDPT Y
Sbjct: 181 LMFNIVMRMMCGKKYCGDDLKDEERSKKFKEMVKQVLAISGAGNPGDFIPLWNWIDPTRY 240

Query: 241 DKKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVL 300
           +K+VKKL+KTSDE IQQLIDEIRNQ+DGENTMIQHLLG QN+QPE YSDQIIKGL+QV+L
Sbjct: 241 EKRVKKLTKTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQVIL 300

Query: 301 LAGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYET 360
           L G D  A TLEWALSHLLNNPEVLKKA DEID SIGQE LV E D  SLSYLQG+I ET
Sbjct: 301 LGGIDTAAVTLEWALSHLLNNPEVLKKAIDEIDSSIGQECLVKEVDSFSLSYLQGIISET 360

Query: 361 LHLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNA 414
           L LNP APLL PHC  EDCKIGGYD+PRDT+VLINAW IHRDPSLWEDATSFKPERH NA
Sbjct: 361 LRLNPAAPLLVPHCAFEDCKIGGYDVPRDTIVLINAWTIHRDPSLWEDATSFKPERHENA 420

[Close]

BLAST of PI0023134 vs. NCBI nr
Match: XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])

HSP 1 Score: 542.3 bits (1396), Expect = 3.5e-150
Identity = 285/494 (57.69%), Postives = 341/494 (69.03%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MD+L LSISL SL SL LAF+FL  S  +NLPPSP FSLP++GHLHL+KHPVH+T H LS
Sbjct: 1   MDILFLSISL-SLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELS 60

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
            K+GHV SLR GSR+VVVVSS S                                     
Sbjct: 61  HKYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPY 120

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDP 180
                         IFSTTRLN+F+G REDEV+ L+ KL + +SF+EF  V++ PM  D 
Sbjct: 121 GEHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCE-NSFEEFTAVELEPMLFDL 180

Query: 181 MLNIVIKMIGGKKY-----------------SIIGIGNPGDFIPLWNWIDPTCYDKKVKK 240
             NIV++M+ GKKY                 + +G  NPGDFIP+WNWIDPT ++K++ K
Sbjct: 181 TFNIVMRMVAGKKYYEEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIK 240

Query: 241 LSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLAGTDA 300
           L + SDE +Q+LIDEIRNQ  G+NTMI HLL  Q ++PEYYSDQIIKGL+QV+LLAG D 
Sbjct: 241 LGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDT 300

Query: 301 VAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLHLNPT 360
            A TLEWALS LLNNPEVL+KA+ EID  +GQERLVNE DLSSL YLQG+I+ETL LNP 
Sbjct: 301 AAVTLEWALSRLLNNPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPA 360

Query: 361 APLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANGVDAH 411
           APLL PHC S DCKIGGYD+PRDT++LIN WAIHRDP+LWEDAT+FKPERHGN  GVD++
Sbjct: 361 APLLIPHCASADCKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSY 420

[Close]

BLAST of PI0023134 vs. NCBI nr
Match: XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 537.0 bits (1382), Expect = 1.5e-148
Identity = 294/501 (58.68%), Postives = 340/501 (67.86%), Query Frame = 0


Query: 1   MDLLLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLS 60
           MD+LLLSISL SL SLF+ F+FL   + +NLPPSP F LPLIGHLHL+KHPVHRTLH LS
Sbjct: 81  MDILLLSISL-SLFSLFIFFHFLLRPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLS 140

Query: 61  QKHGHVLSLRLGSRLVVVVSSSSL------------------------------------ 120
             +GHV SLR GSRLVVVVSS S                                     
Sbjct: 141 HNYGHVFSLRYGSRLVVVVSSPSAVQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPY 200

Query: 121 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDP 180
                         IFST+RLNLF+G REDEVK LL KL   DS QEF+ V++  M +D 
Sbjct: 201 GEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKL-RGDSLQEFRAVELDSMLLDH 260

Query: 181 MLNIVIKMIGGKKY-------------------SII---GIGNPGDFIPLWNWIDPTCYD 240
             NIV++MI GKKY                    I+   G  N GDF+PLWNWIDPT Y+
Sbjct: 261 TFNIVVRMIAGKKYYGEDLRDEGQSKKFREMVKEILANGGTANLGDFVPLWNWIDPTGYE 320

Query: 241 KKVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLL 300
           K++ K+ + SDE +Q+L+DEIRNQ DG NTMI HLL  QN+QPEY+SDQIIKGL Q++LL
Sbjct: 321 KRIMKIGERSDELLQELVDEIRNQKDGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLL 380

Query: 301 AGTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETL 360
           AG D  A TLEWALS LLNNPEVLKKAR EID  IG++RLV+E DLSSLSYLQGVI ETL
Sbjct: 381 AGIDTAAITLEWALSQLLNNPEVLKKARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETL 440

Query: 361 HLNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNA- 412
            LNP APLL PHC SEDCKI GYDIPRDT++LINAWAIHRD ++WEDATSFKPERH N  
Sbjct: 441 RLNPAAPLLVPHCASEDCKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTM 500

[Close]

BLAST of PI0023134 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 357.8 bits (917), Expect = 1.1e-98
Identity = 212/497 (42.66%), Postives = 284/497 (57.14%), Query Frame = 0


Query: 4   LLLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQ-- 63
           L+ SI  + L  ++L           NLPPSP++SLP+IGHL L+K P+HRT  +LSQ  
Sbjct: 6   LIFSILFVVLSLIYLIGKL---KRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSL 65

Query: 64  KHGHVLSLRLGSRLVVVVSSSSL------------------------------------- 123
            +  + SLRLG+RLV V SS S+                                     
Sbjct: 66  NNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYG 125

Query: 124 -------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDPM 183
                        IFS  RLN F+  R+DE++RL+ +L  ++  QEF  V M  M  D  
Sbjct: 126 DHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRL-SRNFSQEFVKVDMKSMLSDLT 185

Query: 184 LNIVIKMIGGKKY----------------------SIIGIGNPGDFIPLWNWIDPTCYDK 243
            N +++M+ GK+Y                      +  G GN  D++P+   +    Y+ 
Sbjct: 186 FNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVSD--YET 245

Query: 244 KVKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLA 303
           +VKKL+   DEF+Q L+DE R   +  NTMI HLL  Q SQP+Y++D+IIKG +  ++LA
Sbjct: 246 RVKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILA 305

Query: 304 GTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLH 363
           GTD  A TLEWALS++LN+P+VL KARDEID  IG +RL++E+D+S+L YLQ ++ ETL 
Sbjct: 306 GTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLR 365

Query: 364 LNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANG 411
           L P AP+L PH  SEDCK+ GYD+PR T++L N WAIHRDP LW+D  SFKPER      
Sbjct: 366 LYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEG- 425

[Close]

BLAST of PI0023134 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 357.1 bits (915), Expect = 1.9e-98
Identity = 210/495 (42.42%), Postives = 284/495 (57.37%), Query Frame = 0


Query: 5   LLSISLISLISLF-LAFNFLHHSHHK-NLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQK 64
           L  I ++S +SLF LA   L  S  K NLPPSP+ SLP+IGHLHL+K P+HRT  +LS+ 
Sbjct: 3   LNQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKS 62

Query: 65  HGH--VLSLRLGSRLVVVVSSSSL------------------------------------ 124
            G+  V  LRLG+RLV V+SS S+                                    
Sbjct: 63  IGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASY 122

Query: 125 --------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDP 184
                         IFST RLN F+  R+DE++RL+  L  +DS   F  V+M  +  + 
Sbjct: 123 GDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHL-SRDSLHGFVEVEMKTLLTNL 182

Query: 185 MLNIVIKMIGGKKY-------------------SIIGIGNPGDFIPLWNWIDPTCYDKKV 244
             N  I+M+ GK+Y                   +  G GNP D++ +  W+  + Y+K++
Sbjct: 183 ASNTTIRMLAGKRYFGEDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRI 242

Query: 245 KKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLAGT 304
           K L    D F+Q+L+DE R + +   TMI HLL  Q+ QP+YY+D IIKG++  +++AGT
Sbjct: 243 KNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGT 302

Query: 305 DAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLHLN 364
           D  + TLEWA+S+LLN+PE+LKKAR EID  +G +RLV+E+D+ +LSYLQ ++ ETL + 
Sbjct: 303 DTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMY 362

Query: 365 PTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANGVD 410
           P  PLL PH  SEDCK+GGYDIP  TMVL NAWA+HRDP +WED   FKPER       +
Sbjct: 363 PAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEG--E 422

[Close]

BLAST of PI0023134 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 355.1 bits (910), Expect = 7.4e-98
Identity = 217/497 (43.66%), Postives = 275/497 (55.33%), Query Frame = 0


Query: 5   LLSISLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQKHG 64
           L+ I     I L L F         NLPPSP+++LP+IGHL L+K P+HR   ++SQ  G
Sbjct: 4   LMLIFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLG 63

Query: 65  H--VLSLRLGSRLVVVVSSSSL-------------------------------------- 124
              ++SLRLG+RL+ VVSS S+                                      
Sbjct: 64  DAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSE 123

Query: 125 ------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDPML 184
                       IFS  RLN F   R DE++RL+ +L    S+  F  V+M  MF D   
Sbjct: 124 HWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSY-GFTKVEMKSMFSDLTF 183

Query: 185 NIVIKMIGGKKY----------------------SIIGIGNPGDFIPLWNWIDPTCYDKK 244
           N +I+M+ GK Y                      S  G GN  D+IP+  WI  T  + +
Sbjct: 184 NNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWI--TYSETR 243

Query: 245 VKKLSKTSDEFIQQLIDEIR-NQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLA 304
           +KKL+   DEF+Q L+DE R  +   ENTM+ HLL  Q +QPEYY D+IIKG +  ++  
Sbjct: 244 IKKLAGRLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAG 303

Query: 305 GTDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLH 364
           GTD  A TLEWALS LLNNPEVL KARDEID  IG +RL+ E+D+ +L YLQ ++ ETL 
Sbjct: 304 GTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLR 363

Query: 365 LNPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANG 410
           L P AP+L PH  S+DCK+GGYD+PR TM+L NAWAIHRDP LW+D TSFKPER      
Sbjct: 364 LYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEG- 423

[Close]

BLAST of PI0023134 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 354.8 bits (909), Expect = 9.7e-98
Identity = 213/490 (43.47%), Postives = 275/490 (56.12%), Query Frame = 0


Query: 14  ISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQKHGH--VLSLRL 73
           ISL L F         NLPPSPS++LP+IGHL L+K P+HR   ++S+  G   ++SLRL
Sbjct: 13  ISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIISLRL 72

Query: 74  GSRLVVVVSSSSL----------------------------------------------- 133
           G+RLV VVSS SL                                               
Sbjct: 73  GNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLRRIG 132

Query: 134 ---IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDPMLNIVIKMIGG 193
              IFS  RLN F   R DE+ RL+  L  ++S  EF  V+M  MF +   N +I+M+ G
Sbjct: 133 AVEIFSAHRLNSFSSIRRDEIHRLIACL-SRNSSLEFTKVEMKSMFSNLTFNNIIRMLAG 192

Query: 194 KKY----------------------SIIGIGNPGDFIPLWNWIDPTCYDKKVKKLSKTSD 253
           K Y                         G GN  D++P+  WI  T  +K++KK++   D
Sbjct: 193 KCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI--TGSEKRIKKIASRLD 252

Query: 254 EFIQQLIDEIR-NQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLAGTDAVAATL 313
           EF+Q L+DE R  +   +NTM+ HLL  Q +QPEYY+D IIKG++  ++LAGTD  A TL
Sbjct: 253 EFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSAVTL 312

Query: 314 EWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLHLNPTAPLLA 373
           EW LS LLN+P++L KARDEID  +G  RLV E+DLS L YLQ ++ E+L L P +PLL 
Sbjct: 313 EWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPLLV 372

Query: 374 PHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANGVDAHKLLPF 411
           PH  SEDCK+GGY +PR TM+L NAWAIHRDP +W+D TSFKPER       +A KLL F
Sbjct: 373 PHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEG--EAQKLLGF 432

[Close]

BLAST of PI0023134 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 353.6 bits (906), Expect = 2.2e-97
Identity = 211/497 (42.45%), Postives = 281/497 (56.54%), Query Frame = 0


Query: 9   SLISLISLFLAFNFLHHSHHKNLPPSPSFSLPLIGHLHLIKHPVHRTLHNLSQKHGH--- 68
           S+ SLI L ++F FL     +NLPPSP   LP+IGHL L+K P+HRTL + S+   H   
Sbjct: 12  SIFSLIFLIISFKFL-KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDG 71

Query: 69  --VLSLRLGSRLVVVVSSSSL--------------------------------------- 128
             V+SLRLGSRLV VVSS  +                                       
Sbjct: 72  GGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGD 131

Query: 129 ------------IFSTTRLNLFMGTREDEVKRLLCKLWDKDSFQEFKVVKMFPMFMDPML 188
                       IFST RLN F+  R DEV+RL+ +L  + +  +  VV++ PM MD   
Sbjct: 132 HWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL-SRLAGTKKTVVELKPMLMDLTF 191

Query: 189 NIVIKMIGGKKY----------------------SIIGIGNPGDFIPLWNWIDPTCYDKK 248
           N +++M+ GK+Y                      +    GN  D++P+      + Y+ +
Sbjct: 192 NNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENR 251

Query: 249 VKKLSKTSDEFIQQLIDEIRNQSDGENTMIQHLLGFQNSQPEYYSDQIIKGLVQVVLLAG 308
           VKKL + +D+F+Q LID+ R Q +   TMI HLL  Q S  EYY+DQIIKG++ ++++AG
Sbjct: 252 VKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAG 311

Query: 309 TDAVAATLEWALSHLLNNPEVLKKARDEIDGSIGQERLVNETDLSSLSYLQGVIYETLHL 368
           T+  A TLEWALS+LLN+P+V+ KARDEID  +G +RL+ E DLS L YL+ ++ ETL L
Sbjct: 312 TNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRL 371

Query: 369 NPTAPLLAPHCVSEDCKIGGYDIPRDTMVLINAWAIHRDPSLWEDATSFKPERHGNANGV 411
           +P  PLL PH  SEDCKIG YD+PR T +L+NAWAIHRDP+ W+D  SFKPER       
Sbjct: 372 HPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEE-- 431

The following BLAST results are available for this feature:

BLAST of PI0023134 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	1.1e-101	42.83	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q9FG65	3.0e-96	42.45	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
P93147	2.0e-95	41.20	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNR0	3.5e-92	40.66	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]
Q6WNQ8	3.5e-92	41.30	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]

BLAST of PI0023134 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BF28	4.3e-178	67.79	Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3C2H7	4.3e-178	67.79	cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495922 PE=3 SV=1	[more]
A0A5A7SSK3	2.8e-177	67.59	Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A6J1D5S1	1.7e-150	57.69	cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV...	[more]
A0A6J1D5S6	7.1e-149	58.68	isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE...	[more]

BLAST of PI0023134 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151923.1	1.2e-179	67.73	cytochrome P450 81Q32 [Cucumis sativus] >KAE8652307.1 hypothetical protein Csa_0...	[more]
XP_008455823.1	8.9e-178	67.79	PREDICTED: cytochrome P450 81D1-like [Cucumis melo] >TYJ98342.1 cytochrome P450 ...	[more]
KAA0032531.1	5.7e-177	67.59	cytochrome P450 81D1-like [Cucumis melo var. makuwa]	[more]
XP_022149455.1	3.5e-150	57.69	cytochrome P450 81E8-like [Momordica charantia]	[more]
XP_022149460.1	1.5e-148	58.68	isoflavone 2'-hydroxylase-like [Momordica charantia]	[more]

BLAST of PI0023134 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	1.1e-98	42.66	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37330.1	1.9e-98	42.42	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT4G37360.1	7.4e-98	43.66	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT4G37340.1	9.7e-98	43.47	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT5G36220.1	2.2e-97	42.45	cytochrome p450 81d1	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 229..246 score: 35.78 coord: 356..365 score: 60.23 coord: 282..293 score: 34.07
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 151..372 e-value: 1.2E-59 score: 202.3 coord: 32..85 e-value: 1.2E-5 score: 24.2
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 60..79 score: 39.88 coord: 355..365 score: 56.69 coord: 322..346 score: 31.29 coord: 281..299 score: 30.99
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 24..374 e-value: 2.2E-85 score: 289.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 32..402
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 8..84 coord: 85..408
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 8..84 coord: 85..408
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 358..367

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0023134.1	PI0023134.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen