PI0022956 (gene) Melon (PI 482460) v1

Overview
NamePI0022956
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr11: 30270629 .. 30271795 (+)
RNA-Seq ExpressionPI0022956
SyntenyPI0022956
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAAAATGTGGCCGTCCAATTCGTATTTGGAAATCTCAACCTCTCAACTTCCATGGAAGCACAAGCTTTCTATTCGTTTCGCTTCTCTACTCTTCAACATTCTTCTACGTTCAAATCTCACCGTCAGTCGTTGGCTTCTCAATTTCATCGACCCCAAAATCCCTCCCAATCTCAATTCAATCCACCCCGTTTTCTCTTCCGATCTCACCATCGACCCTTCTCGACATCTCTTCCTTCGAATCTTCATCCCCACCGCCGCCGAATCACTCCGCCTGCCGCTCATTTTCTTCTTCCACGGTGGCGGCTTTGCCCTCGGCTCTGCCGACTCCACTTCAATGGACCTAGCTGCCACTCTTTTTGCTAAACACCTACCGGGCGTTGTAATTTCCGTCAACTACCGCCTCGCTCCGGAGTTTCGATTCCCCTGCCAATATGACGACGGATTCGACGCTTTGAAATTCATCGACGAAATGGACGACACCCAATTGGTCGGGAGGGCGGATCTGAGCCGTTGCTTCATTTTGGGGGAAAGCGCCGGTGGGAATCTAGGACACCACGTGGCGGTGAGAGCGAGCGAGTACGCGTTCAAGAGAGTGAAGTTAGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAACGAACAGAGTCGGAGATTCGGCTATGTAAACAGCCTTTGTTGAGTTTGAATATTACTGATTGGTTTTGGAAGGCGTTTTTACCGGTTGGGGAAGACCGTAACCATGCAGCGTCGAATGTGAGTGGACCCAATGGAAGGGATATTTTGGGACTGAGGAATTTTCCGGCGACGGTGATTTTCGCGGGAGGATTGGATGTTTTGATTGATCGGCAGAAGAGTTATTATGAGTGGTTAAAGAGAATGGAAAAGGATGTGAAATTGGTGGTTTTCTCGAACGCCGTTCATGGATTCTTTGTCTTTCCAGATTTGCCGGAATATTCTTTGATGGTTGAGGAGATGAAGGATTTCATTGCAAAGTTGAGATAGCACCATGAGTGGTCCAAATTTTATATATAAATTTCAACCTTTAAATCACATTTTTGCCTCAAAAGTTTGTATTTTGATCTCTAAACTATTAAAAACGAAACGTTTCTTTTAGTCATAAACTCAAGGAAAACAACTTATTTTGGTCCCTCTCACTTTT

mRNA sequence

TCAAAATGTGGCCGTCCAATTCGTATTTGGAAATCTCAACCTCTCAACTTCCATGGAAGCACAAGCTTTCTATTCGTTTCGCTTCTCTACTCTTCAACATTCTTCTACGTTCAAATCTCACCGTCAGTCGTTGGCTTCTCAATTTCATCGACCCCAAAATCCCTCCCAATCTCAATTCAATCCACCCCGTTTTCTCTTCCGATCTCACCATCGACCCTTCTCGACATCTCTTCCTTCGAATCTTCATCCCCACCGCCGCCGAATCACTCCGCCTGCCGCTCATTTTCTTCTTCCACGGTGGCGGCTTTGCCCTCGGCTCTGCCGACTCCACTTCAATGGACCTAGCTGCCACTCTTTTTGCTAAACACCTACCGGGCGTTGTAATTTCCGTCAACTACCGCCTCGCTCCGGAGTTTCGATTCCCCTGCCAATATGACGACGGATTCGACGCTTTGAAATTCATCGACGAAATGGACGACACCCAATTGGTCGGGAGGGCGGATCTGAGCCGTTGCTTCATTTTGGGGGAAAGCGCCGGTGGGAATCTAGGACACCACGTGGCGGTGAGAGCGAGCGAGTACGCGTTCAAGAGAGTGAAGTTAGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAACGAACAGAGTCGGAGATTCGGCTATGTAAACAGCCTTTGTTGAGTTTGAATATTACTGATTGGTTTTGGAAGGCGTTTTTACCGGTTGGGGAAGACCGTAACCATGCAGCGTCGAATGTGAGTGGACCCAATGGAAGGGATATTTTGGGACTGAGGAATTTTCCGGCGACGGTGATTTTCGCGGGAGGATTGGATGTTTTGATTGATCGGCAGAAGAGTTATTATGAGTGGTTAAAGAGAATGGAAAAGGATGTGAAATTGGTGGTTTTCTCGAACGCCGTTCATGGATTCTTTGTCTTTCCAGATTTGCCGGAATATTCTTTGATGGTTGAGGAGATGAAGGATTTCATTGCAAAGTTGAGATAGCACCATGAGTGGTCCAAATTTTATATATAAATTTCAACCTTTAAATCACATTTTTGCCTCAAAAGTTTGTATTTTGATCTCTAAACTATTAAAAACGAAACGTTTCTTTTAGTCATAAACTCAAGGAAAACAACTTATTTTGGTCCCTCTCACTTTT

Coding sequence (CDS)

ATGTGGCCGTCCAATTCGTATTTGGAAATCTCAACCTCTCAACTTCCATGGAAGCACAAGCTTTCTATTCGTTTCGCTTCTCTACTCTTCAACATTCTTCTACGTTCAAATCTCACCGTCAGTCGTTGGCTTCTCAATTTCATCGACCCCAAAATCCCTCCCAATCTCAATTCAATCCACCCCGTTTTCTCTTCCGATCTCACCATCGACCCTTCTCGACATCTCTTCCTTCGAATCTTCATCCCCACCGCCGCCGAATCACTCCGCCTGCCGCTCATTTTCTTCTTCCACGGTGGCGGCTTTGCCCTCGGCTCTGCCGACTCCACTTCAATGGACCTAGCTGCCACTCTTTTTGCTAAACACCTACCGGGCGTTGTAATTTCCGTCAACTACCGCCTCGCTCCGGAGTTTCGATTCCCCTGCCAATATGACGACGGATTCGACGCTTTGAAATTCATCGACGAAATGGACGACACCCAATTGGTCGGGAGGGCGGATCTGAGCCGTTGCTTCATTTTGGGGGAAAGCGCCGGTGGGAATCTAGGACACCACGTGGCGGTGAGAGCGAGCGAGTACGCGTTCAAGAGAGTGAAGTTAGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAACGAACAGAGTCGGAGATTCGGCTATGTAAACAGCCTTTGTTGAGTTTGAATATTACTGATTGGTTTTGGAAGGCGTTTTTACCGGTTGGGGAAGACCGTAACCATGCAGCGTCGAATGTGAGTGGACCCAATGGAAGGGATATTTTGGGACTGAGGAATTTTCCGGCGACGGTGATTTTCGCGGGAGGATTGGATGTTTTGATTGATCGGCAGAAGAGTTATTATGAGTGGTTAAAGAGAATGGAAAAGGATGTGAAATTGGTGGTTTTCTCGAACGCCGTTCATGGATTCTTTGTCTTTCCAGATTTGCCGGAATATTCTTTGATGGTTGAGGAGATGAAGGATTTCATTGCAAAGTTGAGATAG

Protein sequence

MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRHLFLRIFIPTAAESLRLPLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGESAGGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFIAKLR
Homology
BLAST of PI0022956 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 5.7e-77
Identity = 147/319 (46.08%), Postives = 204/319 (63.95%), Query Frame = 0

Query: 15  LPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRH 74
           LP K ++++   S + +   R + T++R  L   D + PPN   ++ V +SD  +D SR 
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFIPTAAESLRLPLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNYRLA 134
           L+ R++ P  +   ++P++ FFHGGGFA  S ++   D     FA+ LP  VISVNYRLA
Sbjct: 73  LWFRLYTPHVSGD-KIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRLA 132

Query: 135 PEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGESAGGNLGHHVAV---RASE 194
           PE R+P QYDDGFDALK+I+E   + L   ADLSRCF  G+SAGGN+ H+VA+   R   
Sbjct: 133 PEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICREPR 192

Query: 195 YAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAAS 254
            +F  VKL+G I+ QPFFGGEERTE+E +L   PL+S + TDW WKA   +G +R+H A 
Sbjct: 193 SSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA---MGLNRDHEAV 252

Query: 255 NVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHGFF 314
           NV GPN  DI GL ++P T++   G D L D Q+SYYEWLK   K   L+ + N  H F+
Sbjct: 253 NVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFHAFY 312

Query: 315 VFPDLPEYSLMVEEMKDFI 331
           +FP+LPE   ++  +KDF+
Sbjct: 313 IFPELPEAGQLIMRIKDFV 326

BLAST of PI0022956 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 5.0e-57
Identity = 132/325 (40.62%), Postives = 180/325 (55.38%), Query Frame = 0

Query: 28  LLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRHLFLRI-------- 87
           L +NIL R++ T  R L  ++D ++P N   +  V S D  ID S  L +RI        
Sbjct: 29  LSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGD 88

Query: 88  --------------FIPTAAESLRLPLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGV 147
                         F+  A  +   P+I FFHGG F   SA ST  D     F K   GV
Sbjct: 89  AEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGV 148

Query: 148 VISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGESAGGNLGHHV 207
           V+SVNYR APE R+PC YDDG+ ALK++      +  G A  +R F+ G+S+GGN+ HHV
Sbjct: 149 VVSVNYRRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQ-ARVFLSGDSSGGNIAHHV 208

Query: 208 AVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGED 267
           AVRA++   + VK+ G I     FGG ERTESE RL  +  ++L   DW+WKA+LP   D
Sbjct: 209 AVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDAD 268

Query: 268 RNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSN 327
           R+H A N  GPNGR + GL  F  ++I   GLD+  DRQ +Y + L+     VK+V   N
Sbjct: 269 RDHPACNPFGPNGRRLGGL-PFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCEN 328

Query: 328 AVHGFFVFPDLPEYSLMVEEMKDFI 331
           A  GF++ P+   Y  ++EE+ DF+
Sbjct: 329 ATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of PI0022956 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 6.5e-57
Identity = 123/317 (38.80%), Postives = 183/317 (57.73%), Query Frame = 0

Query: 28  LLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRHLFLRIFIPTAA-- 87
           L +N+L R + T +R L  F+D K+P N N ++ VFS D+ ID   +L  R++ P  A  
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  -----------ESLRLPLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNYRLA 147
                      +   +P+I FFHGG FA  SA+S   D            VV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLS-RCFILGESAGGNLGHHVAVRASEYA 207
           PE R+PC YDDG+  LK+++    + L  + D   R F+ G+S+GGN+ H+VAVRA E  
Sbjct: 149 PENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE-- 208

Query: 208 FKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAASNV 267
             R+ ++G I   P FGG ERTESE RL  +  +++   DW+W+AFLP GEDR H A + 
Sbjct: 209 -SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSP 268

Query: 268 SGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHGFFVF 327
            GP  + + GL +FP +++   GLD++ D Q  Y E LK+  ++VKL+    A  GF++ 
Sbjct: 269 FGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLL 328

Query: 328 PDLPEYSLMVEEMKDFI 331
           P+   +  +++E+  F+
Sbjct: 329 PNNNHFHTVMDEIAAFV 339

BLAST of PI0022956 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.7e-53
Identity = 117/317 (36.91%), Postives = 178/317 (56.15%), Query Frame = 0

Query: 30  FNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRHLFLRIFIPTAAESLR 89
           +NIL R + T +R L  ++D K+  N N +  VFS D+ ID   +L  R++ P  A+  +
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  ---------------LPLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNYRLA 149
                          +P+I FFHGG FA  SA+S   D            VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLS-RCFILGESAGGNLGHHVAVRASEYA 209
           PE  +PC YDDG+ AL +++    + L  + D     F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE-- 210

Query: 210 FKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAASNV 269
              + ++G I   P FGG ERTESE  L  +  +++   DW+WKAFLP GEDR H A N 
Sbjct: 211 -SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 SGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHGFFVF 329
             P G+ + G+ +FP +++   GLD++ D Q +Y E LK+  ++VKL+    A  GF++ 
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 PDLPEYSLMVEEMKDFI 331
           P+   +  +++E+  F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of PI0022956 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.6e-50
Identity = 114/319 (35.74%), Postives = 179/319 (56.11%), Query Frame = 0

Query: 28  LLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRHLFLRIFIP----- 87
           L + +L R + + +R L  F+D K+P N   +  VFS D  +D + +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  -TAAESLRL----------PLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNY 147
            T   +L L          P++ FFHGG F   SA+S   D            VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGESAGGNLGHHVAVRASE 207
           R +PE R+PC YDDG++AL ++      Q  G+      ++ G+S+GGN+ H+VAVRA+ 
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQ-SGKDSNVYVYLAGDSSGGNIAHNVAVRATN 208

Query: 208 YAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAAS 267
              + VK++G I   P FGG+ERT+SE  L  +  +++   DW+W+A+LP GEDR+H A 
Sbjct: 209 ---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPAC 268

Query: 268 NVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHGFF 327
           N  GP G+ + G+ NFP +++   GLD++ D Q +Y + LK+   +V L+    A  GF+
Sbjct: 269 NPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFY 328

Query: 328 VFPDLPEYSLMVEEMKDFI 331
             P+   +  ++EE+  F+
Sbjct: 329 FLPNNDHFHCLMEELNKFV 341

BLAST of PI0022956 vs. ExPASy TrEMBL
Match: A0A6J1ECC8 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.6e-146
Identity = 257/334 (76.95%), Postives = 293/334 (87.72%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M PS+   EISTSQLPWKH+L++RFA+LLFN  +RS+LTV+R LLNF+DPKIPPN NSIH
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFIPT--AAESLRLPLIFFFHGGGFALGSADSTSMDLAATLF 120
            V SSDLTID SR LFLRIF PT  A +S+  P+IF+FHGGGFA GSAD+TSMD+AA  F
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSADATSMDMAARRF 120

Query: 121 AKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQ-LVGRADLSRCFILGESA 180
           +K L  VVISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDD   L+G+ADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWK 240
           GGNLGHHVAVRASEYAFKRVK++GFIASQPFFGGEERTESEIRLC QP L+L + DWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKD 300
           AFLP GEDR+HAA+NV+GPNGRD+ GLRNFPAT+I AGGLD+LIDRQ+ YYE LKRM K+
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKE 300

Query: 301 VKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFIA 332
           VKLVVF+N VHGFF F D PEYSLM++EM+DFIA
Sbjct: 301 VKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of PI0022956 vs. ExPASy TrEMBL
Match: A0A6J1IER7 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 1.0e-145
Identity = 257/333 (77.18%), Postives = 290/333 (87.09%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M P +   EISTSQLPWKH+L++RFA+LLFN  +RS+LTV+R LLNF+DPKIPPN NSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFIPT--AAESLRLPLIFFFHGGGFALGSADSTSMDLAATLF 120
            V SSDLTID SR LFLRIF PT  A +SL  P+IF+FHGGGFA GSA +TSMD+AA  F
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPTVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120

Query: 121 AKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQ-LVGRADLSRCFILGESA 180
           +K L  VVISVNYRL+P+FRFPCQYDDGFDA+KFIDEMDD   L+GRADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWK 240
           GGNLGHHVAVRASEYAFKRVK++GFIASQPFFGGEERTESEIRLC QP L+L + DWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKD 300
           AFLP GEDR+HAA+NV+GPNGRD+ GLRNFPAT+I AGGLD LIDRQ+ YYE LKRM KD
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKD 300

Query: 301 VKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFI 331
           VKLVVF+N VHGFF F DLPEYSLM++EM+DFI
Sbjct: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 333

BLAST of PI0022956 vs. ExPASy TrEMBL
Match: A0A0A0KXS8 (Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.9e-144
Identity = 263/338 (77.81%), Postives = 290/338 (85.80%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M  S+   E+ST QLPWKHK+++RFA+LLFN  LRS+ TV+R LL F+DPKIPPN NS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFI--PTAA--ESL-RLPLIFFFHGGGFALGSADSTSMDLAA 120
            V SSDLTID SR LFLRIF   PTAA  ESL  LP+IF+FHGGGFA GSAD+TS D+AA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 TLFAKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGE 180
             FA+ L  VVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD  L+ R DLSRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLCKQ  L+L +TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WKAFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRME 300
           W+AFLP GEDR+HAA+NV+GPNGRDI GL NFPATVIFAGGLD+L+DRQKSYYE LKRM 
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFIAKL 334
           KDVKLVVFSNA HGFF FPDLPEYSLM+EEM DFIAKL
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

BLAST of PI0022956 vs. ExPASy TrEMBL
Match: A0A1S3CBA4 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 5.8e-141
Identity = 251/337 (74.48%), Postives = 284/337 (84.27%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M  S    +IST QLPWKH++++RFA+LL+N  LRS+LTV+R LLNFIDPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFIPTAAESL-----RLPLIFFFHGGGFALGSADSTSMDLAA 120
            V SSDL +D SR LFLRIF PT   +L      LP+IF+FHGGGF  GSAD+TS D+AA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 TLFAKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGE 180
             FAK L  VVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD  L+ R DLS CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLCKQ  L+L++TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WKAFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRME 300
           W+AFLP GEDR+HAA+NV+GPNGRDI GL NFPAT+IFAGGLD+LIDRQK YYE LKRM 
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFIAK 333
           KDVKLVVFSNAVHG+F F DLPEYSLM++EM+DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of PI0022956 vs. ExPASy TrEMBL
Match: A0A5D3BL91 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001330 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.1e-139
Identity = 249/337 (73.89%), Postives = 282/337 (83.68%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M  S    +IST QLPWKH++++RFA+LL+N  LRS+LTV+R LLNFIDPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFIPTAAESL-----RLPLIFFFHGGGFALGSADSTSMDLAA 120
            V SSDL +D SR LFLRIF PT   +L      LP+IF+FHGGGF  GSAD+TS D+AA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 TLFAKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGE 180
             FAK L  VVISVNYRLAPEFRFPCQ DDGFD LKFIDEMDD  L+ R DLSRCFILGE
Sbjct: 121 RRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLCKQ  L+L++TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WKAFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRME 300
           W+AFLP GEDR+HA +NV+GPNGRDI GL NFPAT+IFAGGLD+LIDRQK YYE LKRM 
Sbjct: 241 WRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFIAK 333
           KDVKLVVFSNAVHG+F F DLPEYSLM++EM+DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of PI0022956 vs. NCBI nr
Match: XP_022925491.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 528.9 bits (1361), Expect = 3.2e-146
Identity = 257/334 (76.95%), Postives = 293/334 (87.72%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M PS+   EISTSQLPWKH+L++RFA+LLFN  +RS+LTV+R LLNF+DPKIPPN NSIH
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFIPT--AAESLRLPLIFFFHGGGFALGSADSTSMDLAATLF 120
            V SSDLTID SR LFLRIF PT  A +S+  P+IF+FHGGGFA GSAD+TSMD+AA  F
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSADATSMDMAARRF 120

Query: 121 AKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQ-LVGRADLSRCFILGESA 180
           +K L  VVISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDD   L+G+ADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWK 240
           GGNLGHHVAVRASEYAFKRVK++GFIASQPFFGGEERTESEIRLC QP L+L + DWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKD 300
           AFLP GEDR+HAA+NV+GPNGRD+ GLRNFPAT+I AGGLD+LIDRQ+ YYE LKRM K+
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKE 300

Query: 301 VKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFIA 332
           VKLVVF+N VHGFF F D PEYSLM++EM+DFIA
Sbjct: 301 VKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of PI0022956 vs. NCBI nr
Match: KAG6592331.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025153.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 526.9 bits (1356), Expect = 1.2e-145
Identity = 256/334 (76.65%), Postives = 292/334 (87.43%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M P +   EISTSQLPWKH+L++RFA+LLFN  +RS+LTV+R LLNF+DPKIPPN NSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFIPT--AAESLRLPLIFFFHGGGFALGSADSTSMDLAATLF 120
            V SSDLTID SR LFLRIF PT  A +S+  P+IF+FHGGGFA GSA +TSMD+AA  F
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120

Query: 121 AKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQ-LVGRADLSRCFILGESA 180
           +K L  VVISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDD   L+G+ADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWK 240
           GGNLGHHVAVRASEYAFKRVK++GFIASQPFFGGEERTESEIRLC QP L+L + DWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKD 300
           AFLP GEDR+HAA+NV+GPNGRD+ GLRNFPAT+I AGGLD+LIDRQ+ YYE LKRM K+
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKE 300

Query: 301 VKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFIA 332
           VKLVVF+N VHGFF F DLPEYSLM++EM+DFIA
Sbjct: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 334

BLAST of PI0022956 vs. NCBI nr
Match: XP_022973499.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 526.2 bits (1354), Expect = 2.1e-145
Identity = 257/333 (77.18%), Postives = 290/333 (87.09%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M P +   EISTSQLPWKH+L++RFA+LLFN  +RS+LTV+R LLNF+DPKIPPN NSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFIPT--AAESLRLPLIFFFHGGGFALGSADSTSMDLAATLF 120
            V SSDLTID SR LFLRIF PT  A +SL  P+IF+FHGGGFA GSA +TSMD+AA  F
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPTVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRF 120

Query: 121 AKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQ-LVGRADLSRCFILGESA 180
           +K L  VVISVNYRL+P+FRFPCQYDDGFDA+KFIDEMDD   L+GRADLSRCFILGESA
Sbjct: 121 SKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWK 240
           GGNLGHHVAVRASEYAFKRVK++GFIASQPFFGGEERTESEIRLC QP L+L + DWFWK
Sbjct: 181 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 240

Query: 241 AFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKD 300
           AFLP GEDR+HAA+NV+GPNGRD+ GLRNFPAT+I AGGLD LIDRQ+ YYE LKRM KD
Sbjct: 241 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKD 300

Query: 301 VKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFI 331
           VKLVVF+N VHGFF F DLPEYSLM++EM+DFI
Sbjct: 301 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 333

BLAST of PI0022956 vs. NCBI nr
Match: XP_023536262.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523.5 bits (1347), Expect = 1.4e-144
Identity = 256/342 (74.85%), Postives = 292/342 (85.38%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M P +   EISTSQLPWKH+L++RFA+LLFN  +RS+LTV+R LLNF+DPKIPPN NSIH
Sbjct: 1   MQPFSVSSEISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFIPT----------AAESLRLPLIFFFHGGGFALGSADSTS 120
            V SSDLTID SR LFLRIF PT          A +SL  P+IF+FHGGGFA GSA +TS
Sbjct: 61  AVSSSDLTIDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATS 120

Query: 121 MDLAATLFAKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQ-LVGRADLSR 180
           MD+AA  F+K L  V+ISVNYRLAP+FRFPCQYDDGFDA+KFIDEMDD   L+G+ADLSR
Sbjct: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180

Query: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSL 240
           CFILGESAGGNLGHHVAVRASEYAFKRVK++GFIASQPFFGGEERTESEIRLC QP L+L
Sbjct: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240

Query: 241 NITDWFWKAFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYE 300
            + DWFWKAFLP GEDR+HAA+NV+GPNGRD+ GLRNFPAT+I AGGLD+LIDRQ+ YYE
Sbjct: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300

Query: 301 WLKRMEKDVKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFIA 332
            LKRM K+VKLVVF+N VHGFF F DLPEYSLM++EM+DFIA
Sbjct: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 342

BLAST of PI0022956 vs. NCBI nr
Match: XP_004141618.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein Csa_008680 [Cucumis sativus])

HSP 1 Score: 521.9 bits (1343), Expect = 4.0e-144
Identity = 263/338 (77.81%), Postives = 290/338 (85.80%), Query Frame = 0

Query: 1   MWPSNSYLEISTSQLPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIH 60
           M  S+   E+ST QLPWKHK+++RFA+LLFN  LRS+ TV+R LL F+DPKIPPN NS H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  PVFSSDLTIDPSRHLFLRIFI--PTAA--ESL-RLPLIFFFHGGGFALGSADSTSMDLAA 120
            V SSDLTID SR LFLRIF   PTAA  ESL  LP+IF+FHGGGFA GSAD+TS D+AA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 TLFAKHLPGVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGE 180
             FA+ L  VVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD  L+ R DLSRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLCKQ  L+L +TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WKAFLPVGEDRNHAASNVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRME 300
           W+AFLP GEDR+HAA+NV+GPNGRDI GL NFPATVIFAGGLD+L+DRQKSYYE LKRM 
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFFVFPDLPEYSLMVEEMKDFIAKL 334
           KDVKLVVFSNA HGFF FPDLPEYSLM+EEM DFIAKL
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

BLAST of PI0022956 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 289.3 bits (739), Expect = 4.0e-78
Identity = 147/319 (46.08%), Postives = 204/319 (63.95%), Query Frame = 0

Query: 15  LPWKHKLSIRFASLLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRH 74
           LP K ++++   S + +   R + T++R  L   D + PPN   ++ V +SD  +D SR 
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFIPTAAESLRLPLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNYRLA 134
           L+ R++ P  +   ++P++ FFHGGGFA  S ++   D     FA+ LP  VISVNYRLA
Sbjct: 73  LWFRLYTPHVSGD-KIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVNYRLA 132

Query: 135 PEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGESAGGNLGHHVAV---RASE 194
           PE R+P QYDDGFDALK+I+E   + L   ADLSRCF  G+SAGGN+ H+VA+   R   
Sbjct: 133 PEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRICREPR 192

Query: 195 YAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAAS 254
            +F  VKL+G I+ QPFFGGEERTE+E +L   PL+S + TDW WKA   +G +R+H A 
Sbjct: 193 SSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA---MGLNRDHEAV 252

Query: 255 NVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHGFF 314
           NV GPN  DI GL ++P T++   G D L D Q+SYYEWLK   K   L+ + N  H F+
Sbjct: 253 NVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMFHAFY 312

Query: 315 VFPDLPEYSLMVEEMKDFI 331
           +FP+LPE   ++  +KDF+
Sbjct: 313 IFPELPEAGQLIMRIKDFV 326

BLAST of PI0022956 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 222.6 bits (566), Expect = 4.6e-58
Identity = 123/317 (38.80%), Postives = 183/317 (57.73%), Query Frame = 0

Query: 28  LLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRHLFLRIFIPTAA-- 87
           L +N+L R + T +R L  F+D K+P N N ++ VFS D+ ID   +L  R++ P  A  
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  -----------ESLRLPLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNYRLA 147
                      +   +P+I FFHGG FA  SA+S   D            VV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLS-RCFILGESAGGNLGHHVAVRASEYA 207
           PE R+PC YDDG+  LK+++    + L  + D   R F+ G+S+GGN+ H+VAVRA E  
Sbjct: 149 PENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE-- 208

Query: 208 FKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAASNV 267
             R+ ++G I   P FGG ERTESE RL  +  +++   DW+W+AFLP GEDR H A + 
Sbjct: 209 -SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSP 268

Query: 268 SGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHGFFVF 327
            GP  + + GL +FP +++   GLD++ D Q  Y E LK+  ++VKL+    A  GF++ 
Sbjct: 269 FGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLL 328

Query: 328 PDLPEYSLMVEEMKDFI 331
           P+   +  +++E+  F+
Sbjct: 329 PNNNHFHTVMDEIAAFV 339

BLAST of PI0022956 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 209.5 bits (532), Expect = 4.1e-54
Identity = 117/317 (36.91%), Postives = 178/317 (56.15%), Query Frame = 0

Query: 30  FNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRHLFLRIFIPTAAESLR 89
           +NIL R + T +R L  ++D K+  N N +  VFS D+ ID   +L  R++ P  A+  +
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  ---------------LPLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNYRLA 149
                          +P+I FFHGG FA  SA+S   D            VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLS-RCFILGESAGGNLGHHVAVRASEYA 209
           PE  +PC YDDG+ AL +++    + L  + D     F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE-- 210

Query: 210 FKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAASNV 269
              + ++G I   P FGG ERTESE  L  +  +++   DW+WKAFLP GEDR H A N 
Sbjct: 211 -SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 SGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHGFFVF 329
             P G+ + G+ +FP +++   GLD++ D Q +Y E LK+  ++VKL+    A  GF++ 
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 PDLPEYSLMVEEMKDFI 331
           P+   +  +++E+  F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of PI0022956 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-51
Identity = 114/319 (35.74%), Postives = 179/319 (56.11%), Query Frame = 0

Query: 28  LLFNILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRHLFLRIFIP----- 87
           L + +L R + + +R L  F+D K+P N   +  VFS D  +D + +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  -TAAESLRL----------PLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNY 147
            T   +L L          P++ FFHGG F   SA+S   D            VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFIDEMDDTQLVGRADLSRCFILGESAGGNLGHHVAVRASE 207
           R +PE R+PC YDDG++AL ++      Q  G+      ++ G+S+GGN+ H+VAVRA+ 
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQ-SGKDSNVYVYLAGDSSGGNIAHNVAVRATN 208

Query: 208 YAFKRVKLVGFIASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAAS 267
              + VK++G I   P FGG+ERT+SE  L  +  +++   DW+W+A+LP GEDR+H A 
Sbjct: 209 ---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPAC 268

Query: 268 NVSGPNGRDILGLRNFPATVIFAGGLDVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHGFF 327
           N  GP G+ + G+ NFP +++   GLD++ D Q +Y + LK+   +V L+    A  GF+
Sbjct: 269 NPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFY 328

Query: 328 VFPDLPEYSLMVEEMKDFI 331
             P+   +  ++EE+  F+
Sbjct: 329 FLPNNDHFHCLMEELNKFV 341

BLAST of PI0022956 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 176.0 bits (445), Expect = 5.0e-44
Identity = 104/289 (35.99%), Postives = 158/289 (54.67%), Query Frame = 0

Query: 31  NILLRSNLTVSRWLLNFIDPKIPPNLNSIHPVFSSDLTIDPSRHLFLRIFIPTAA----- 90
           NI+   + +++R L NF      P+ + ++P  S DL ++  +  +LR+++P++A     
Sbjct: 15  NIVNNPDGSITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGN 74

Query: 91  -ESLRLPLIFFFHGGGFALGSADSTSMDLAATLFAKHLPGVVISVNYRLAPEFRFPCQYD 150
             S +LP++ ++HGGGF L S D        +  A+ L  +V+S +YRLAPE R P  YD
Sbjct: 75  VSSQKLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYD 134

Query: 151 DGFDALKFIDEMDDTQLVGRADLSRCFILGESAGGNLGHHVAVRA--SEYAFKRVKLVGF 210
           DG +AL +I   DD  +   AD S  F++G SAGGNL ++V +R+  S      +++ G 
Sbjct: 135 DGVEALDWIKTSDDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGL 194

Query: 211 IASQPFFGGEERTESEIRLCKQPLLSLNITDWFWKAFLPVGEDRNHAASNVSGPNGRDIL 270
           I   PFFGGEER+ESEIRL    +    +TD  W   LPVG DR+H  SN +  +G + L
Sbjct: 195 ILHHPFFGGEERSESEIRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKL 254

Query: 271 -GLRNFPATVIFAGGL-DVLIDRQKSYYEWLKRMEKDVKLVVFSNAVHG 310
             +      V+  GG  D +ID QK   + +K+   +V        VHG
Sbjct: 255 EKIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHG 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT105.7e-7746.08Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q6L5455.0e-5740.62Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q940G66.5e-5738.80Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q9MAA75.7e-5336.91Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q9LYC11.6e-5035.74Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ECC81.6e-14676.95probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 ... [more]
A0A6J1IER71.0e-14577.18probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV... [more]
A0A0A0KXS81.9e-14477.81Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1[more]
A0A1S3CBA45.8e-14174.48probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1[more]
A0A5D3BL911.1e-13973.89Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
XP_022925491.13.2e-14676.95probable carboxylesterase 18 [Cucurbita moschata][more]
KAG6592331.11.2e-14576.65putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022973499.12.1e-14577.18probable carboxylesterase 18 [Cucurbita maxima][more]
XP_023536262.11.4e-14474.85probable carboxylesterase 18 [Cucurbita pepo subsp. pepo][more]
XP_004141618.14.0e-14477.81probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein ... [more]
Match NameE-valueIdentityDescription
AT5G23530.14.0e-7846.08carboxyesterase 18 [more]
AT5G27320.14.6e-5838.80alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.14.1e-5436.91alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.11.1e-5135.74alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.15.0e-4435.99carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 93..311
e-value: 2.1E-49
score: 168.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 4..331
e-value: 3.8E-80
score: 271.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 62..333
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 12..331
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 12..331
IPR002168Lipase, GDXG, putative histidine active sitePROSITEPS01173LIPASE_GDXG_HIScoord: 93..109

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0022956.1PI0022956.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity