PI0022818 (gene) Melon (PI 482460) v1

Overview


Name	PI0022818
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Trypsin inhibitor
Location	chr08: 2327105 .. 2327553 (-)
RNA-Seq Expression	PI0022818
Synteny	PI0022818

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCACACCAAACTTTGTAAGCTCACAAATAGAAAGATAACAATTCCGAGATCATGTCGTCCCCATCCGTTCGTAAGTATATCTTTTGGTGTAATTTTTTGTGTTGAGTTCATCTCTTTTCTTCCAAGTTATATATATATACACTTTTTAATCTTTGCTAATTAACAATAATATATATACTTTTTGCCAATATGTTTCGAAACAGTAAAATCATGGCCAGAACTAAACTTCATAGATGCAAACACTGTGGCAAATATCATCAAGAGAGAGAATCCTAACTTTCAACCAGTCATATTGTTGGCAGGAAGTCCAGTAACAAAGGATCTTAAGCCTGGTCGAGTTCGACTCTTTACTAATGTGCAGGGAATAGTGGTTATTGTTCCTCAAGAGGGCTGAAATGTAAACATACATGTTTCAAATAATAAATAAAAGTAATAATCTATTTCTACT

mRNA sequence

CCCACACCAAACTTTGTAAGCTCACAAATAGAAAGATAACAATTCCGAGATCATGTCGTCCCCATCCGTTCTAAAATCATGGCCAGAACTAAACTTCATAGATGCAAACACTGTGGCAAATATCATCAAGAGAGAGAATCCTAACTTTCAACCAGTCATATTGTTGGCAGGAAGTCCAGTAACAAAGGATCTTAAGCCTGGTCGAGTTCGACTCTTTACTAATGTGCAGGGAATAGTGGTTATTGTTCCTCAAGAGGGCTGAAATGTAAACATACATGTTTCAAATAATAAATAAAAGTAATAATCTATTTCTACT

Coding sequence (CDS)

ATGTCGTCCCCATCCGTTCTAAAATCATGGCCAGAACTAAACTTCATAGATGCAAACACTGTGGCAAATATCATCAAGAGAGAGAATCCTAACTTTCAACCAGTCATATTGTTGGCAGGAAGTCCAGTAACAAAGGATCTTAAGCCTGGTCGAGTTCGACTCTTTACTAATGTGCAGGGAATAGTGGTTATTGTTCCTCAAGAGGGCTGA

Protein sequence

MSSPSVLKSWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNVQGIVVIVPQEG

Homology

[Close]

BLAST of PI0022818 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.5e-08
Identity = 28/61 (45.90%), Postives = 40/61 (65.57%), Query Frame = 0


Query: 9  SWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNVQGIVVIVPQE 68
          SWP L  +  +    II+R+NPN + VIL  G+PVTKD +  RVR++ N +G+VV  P+ 
Sbjct: 8  SWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSPPRI 67

Query: 69 G 70
          G
Sbjct: 68 G 68

[Close]

BLAST of PI0022818 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 7.5e-08
Identity = 30/59 (50.85%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 10  WPELNFIDANTVANIIKRENPNFQPV-ILLAGSPVTKDLKPGRVRLFTNVQGIVVIVPQ 68
           WPEL  + A     II++ENP+   + ILL GSPVTKD +  RVRLF N+ G VV +P+
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIPR 109

[Close]

BLAST of PI0022818 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.7e-07
Identity = 33/68 (48.53%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 4   PSVLKS-WPELNFIDANTVANIIKRENPNFQPV-ILLAGSPVTKDLKPGRVRLFTNVQGI 63
           P V K  WPEL    A     II++ENP    V  +L G+PVT+DL+  RVRLF NV   
Sbjct: 40  PGVTKERWPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDF 99

Query: 64  VVIVPQEG 70
           VV  PQ G
Sbjct: 100 VVQTPQVG 107

[Close]

BLAST of PI0022818 vs. ExPASy Swiss-Prot
Match: Q03198 (Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 6.3e-07
Identity = 32/68 (47.06%), Postives = 38/68 (55.88%), Query Frame = 0


Query: 4   PSVLKS-WPELNFIDANTVANIIKRENPNFQPV-ILLAGSPVTKDLKPGRVRLFTNVQGI 63
           P V K  WPEL    A     II++ENP    V  +L G PVT+DL+  RVRLF NV   
Sbjct: 40  PGVTKERWPELLGTPAKFAMQIIQKENPKLTNVQTILNGGPVTEDLRCNRVRLFVNVLDF 99

Query: 64  VVIVPQEG 70
           +V  PQ G
Sbjct: 100 IVQTPQIG 107

[Close]

BLAST of PI0022818 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 1.8e-06
Identity = 27/58 (46.55%), Postives = 36/58 (62.07%), Query Frame = 0


Query: 10  WPELNFIDANTVANIIKRENPNFQPV-ILLAGSPVTKDLKPGRVRLFTNVQGIVVIVP 67
           WPEL  +       II++ENP+   + ILL+GSP+T D    RVRLF N+ G VV +P
Sbjct: 51  WPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQMP 108

[Close]

BLAST of PI0022818 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.0e-20
Identity = 56/71 (78.87%), Postives = 61/71 (85.92%), Query Frame = 0


Query: 1  MSSPS--VLKSWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNV 60
          MSS S   LKSWP LNF+DA TVANIIK+ENP+FQ  ILLAGSPVTKDLK GRVRLFTN+
Sbjct: 1  MSSASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNM 60

Query: 61 QGIVVIVPQEG 70
          + IVVIVPQEG
Sbjct: 61 EEIVVIVPQEG 71

[Close]

BLAST of PI0022818 vs. ExPASy TrEMBL
Match: A0A0A0LFD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-14
Identity = 44/69 (63.77%), Postives = 50/69 (72.46%), Query Frame = 0


Query: 1  MSSPSVLKSWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNVQG 60
          MS P  LKSWP L F+DA TVAN IK ENPNF+ V +  G+PVTKD+ PGRVRL  +  G
Sbjct: 1  MSFPFNLKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANG 60

Query: 61 IVVIVPQEG 70
           VV VPQEG
Sbjct: 61 KVVNVPQEG 69

[Close]

BLAST of PI0022818 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.9e-10
Identity = 32/61 (52.46%), Postives = 46/61 (75.41%), Query Frame = 0


Query: 9  SWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNVQGIVVIVPQE 68
          SWPEL F+ ++   + I+R+ P+ +PV+LLAGSPVT+DL+P RVR+F N+   VV VP+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 69 G 70
          G
Sbjct: 75 G 75

[Close]

BLAST of PI0022818 vs. ExPASy TrEMBL
Match: A0A6J1CY53 (inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=LOC111015335 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-09
Identity = 37/79 (46.84%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 1  MSSPSVLKS----------WPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPG 60
          MS+PS L+S          WPEL  +  +  A IIKREN   +P +LLAGSPVT D +P 
Sbjct: 1  MSAPSDLQSSGLEVPGRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPY 60

Query: 61 RVRLFTNVQGIVVIVPQEG 70
          RVR+F N++ +VV +P+ G
Sbjct: 61 RVRIFKNIEDVVVGIPRTG 79

[Close]

BLAST of PI0022818 vs. ExPASy TrEMBL
Match: A0A397ZK38 (Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.6e-07
Identity = 31/63 (49.21%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 7  LKSWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNVQGIVVIVP 66
          LK+WPEL     +  A++IKRENP+F   +LL GS VT DL+  RVR++ +   IVV VP
Sbjct: 3  LKTWPELVGTHGDDAASVIKRENPSFNVFVLLIGSIVTADLRENRVRVWVDTNRIVVRVP 62

Query: 67 QEG 70
            G
Sbjct: 63 TTG 65

[Close]

BLAST of PI0022818 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 107.1 bits (266), Expect = 6.3e-20
Identity = 56/71 (78.87%), Postives = 61/71 (85.92%), Query Frame = 0


Query: 1  MSSPS--VLKSWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNV 60
          MSS S   LKSWP LNF+DA TVANIIK+ENP+FQ  ILLAGSPVTKDLK GRVRLFTN+
Sbjct: 1  MSSASHHQLKSWPALNFMDARTVANIIKKENPDFQIFILLAGSPVTKDLKHGRVRLFTNM 60

Query: 61 QGIVVIVPQEG 70
          + IVVIVPQEG
Sbjct: 61 EEIVVIVPQEG 71

[Close]

BLAST of PI0022818 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 99.8 bits (247), Expect = 1.0e-17
Identity = 48/64 (75.00%), Postives = 55/64 (85.94%), Query Frame = 0


Query: 6  VLKSWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNVQGIVVIV 65
          VLKSWPELN + A+TV NIIKR++P FQ VILLAGSPVTKDLK GRVRLF N+ GIVV +
Sbjct: 4  VLKSWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVTKDLKHGRVRLFVNILGIVVEI 63

Query: 66 PQEG 70
          P+EG
Sbjct: 64 PREG 67

[Close]

BLAST of PI0022818 vs. NCBI nr
Match: KGN60478.1 (hypothetical protein Csa_001396 [Cucumis sativus])

HSP 1 Score: 88.6 bits (218), Expect = 2.3e-14
Identity = 44/69 (63.77%), Postives = 50/69 (72.46%), Query Frame = 0


Query: 1  MSSPSVLKSWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNVQG 60
          MS P  LKSWP L F+DA TVAN IK ENPNF+ V +  G+PVTKD+ PGRVRL  +  G
Sbjct: 1  MSFPFNLKSWPALKFLDAQTVANAIKTENPNFEIVTVPEGTPVTKDIIPGRVRLVVDANG 60

Query: 61 IVVIVPQEG 70
           VV VPQEG
Sbjct: 61 KVVNVPQEG 69

[Close]

BLAST of PI0022818 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 73.2 bits (178), Expect = 1.0e-09
Identity = 32/61 (52.46%), Postives = 46/61 (75.41%), Query Frame = 0


Query: 9  SWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNVQGIVVIVPQE 68
          SWPEL F+ ++   + I+R+ P+ +PV+LLAGSPVT+DL+P RVR+F N+   VV VP+ 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEVPRT 74

Query: 69 G 70
          G
Sbjct: 75 G 75

[Close]

BLAST of PI0022818 vs. NCBI nr
Match: XP_022146026.1 (inhibitor of trypsin and hageman factor-like [Momordica charantia])

HSP 1 Score: 72.0 bits (175), Expect = 2.2e-09
Identity = 37/79 (46.84%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 1  MSSPSVLKS----------WPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPG 60
          MS+PS L+S          WPEL  +  +  A IIKREN   +P +LLAGSPVT D +P 
Sbjct: 1  MSAPSDLQSSGLEVPGRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPY 60

Query: 61 RVRLFTNVQGIVVIVPQEG 70
          RVR+F N++ +VV +P+ G
Sbjct: 61 RVRIFKNIEDVVVGIPRTG 79

[Close]

BLAST of PI0022818 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 57.0 bits (136), Expect = 7.0e-09
Identity = 27/61 (44.26%), Postives = 37/61 (60.66%), Query Frame = 0


Query: 9  SWPELNFIDANTVANIIKRENPNFQPVILLAGSPVTKDLKPGRVRLFTNVQGIVVIVPQE 68
          SWPEL   + +  A +I+RENP     ++L GSPVT D +  RVR+F +   IVV  P+ 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTPKS 69

Query: 69 G 70
          G
Sbjct: 70 G 70

The following BLAST results are available for this feature:

BLAST of PI0022818 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	1.5e-08	45.90	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P16231	7.5e-08	50.85	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
Q03199	3.7e-07	48.53	Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1	[more]
Q03198	6.3e-07	47.06	Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1	[more]
P05118	1.8e-06	46.55	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]

BLAST of PI0022818 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIF6	3.0e-20	78.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1	[more]
A0A0A0LFD4	1.1e-14	63.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914580 PE=3 SV=1	[more]
A0A0A0KPI0	4.9e-10	52.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A6J1CY53	1.1e-09	46.84	inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A397ZK38	6.6e-07	49.21	Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRARA_D01163 PE=3 SV=1	[more]

BLAST of PI0022818 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN60477.1	6.3e-20	78.87	hypothetical protein Csa_001998 [Cucumis sativus]	[more]
KAG6571807.1	1.0e-17	75.00	hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN60478.1	2.3e-14	63.77	hypothetical protein Csa_001396 [Cucumis sativus]	[more]
XP_011654473.1	1.0e-09	52.46	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
XP_022146026.1	2.2e-09	46.84	inhibitor of trypsin and hageman factor-like [Momordica charantia]	[more]

BLAST of PI0022818 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	7.0e-09	44.26	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 34..45 score: 47.1 coord: 46..56 score: 49.01 coord: 9..22 score: 31.99
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..67 e-value: 2.5E-15 score: 56.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 3..69
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 5..67 e-value: 2.3E-16 score: 61.8
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 3..69
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 5..67

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0022818.1	PI0022818.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity