PI0022653 (gene) Melon (PI 482460) v1

BLAST of PI0022653 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 560/744 (75.27%), Postives = 654/744 (87.90%), Query Frame = 0

Query: 35  KTFIFRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHP 94
           KTFIFRID  S PS+FPTHYHWY++EF +  +I+HVY TVFHGFSA +T D+ D++  HP
Sbjct: 26  KTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHP 85

Query: 95  SVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVN 154
           +VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGSDVIIGVFDTGI PERRSFSD+N
Sbjct: 86  AVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLN 145

Query: 155 LGPIPRRWKGVCETGTKFTATNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPR 214
           LGPIP+RW+GVCE+G +F+  NCNRKI+GARFF+KG +A       I GIN T+E+ SPR
Sbjct: 146 LGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAV-----IGGINKTVEFLSPR 205

Query: 215 DADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAA 274
           DADGHGTHT+STAAGRH+F+AS+ GYASG+AKGVAPKAR+A YKVCWK+SGC DSDILAA
Sbjct: 206 DADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAA 265

Query: 275 FDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVT 334
           FDAAV DGVDVISISIGGGDG++SPYYLDPIAIG+YGAASKG+FVSSSAGN+GPNGMSVT
Sbjct: 266 FDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVT 325

Query: 335 NLAPWVTTVGAGTIDRNFPAVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVS 394
           NLAPWVTTVGA TIDRNFPA   LG+G ++ GVSLYAG PLNG M+P+VYPGKSG+ S S
Sbjct: 326 NLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS 385

Query: 395 LCMENSLDPKVVSGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLL 454
           LCMEN+LDPK V GKIVICDRGSSPRVAKGLVVKKAGGVGMILANG SNGEGLVGDAHL+
Sbjct: 386 LCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLI 445

Query: 455 PACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKP 514
           PACAVGS+EGD +KAYASS  NP A+I F+GTI+GIKPAPV+ASFS RGPNGL+PEILKP
Sbjct: 446 PACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKP 505

Query: 515 DIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSP 574
           D+IAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSP
Sbjct: 506 DLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSP 565

Query: 575 AALRSAMMTTASITDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYI 634
           A +RSAMMTT ++ DN  + + +ESTGK +TPYD+G+GH+NLG AM+PGL+YDI+N DYI
Sbjct: 566 AVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYI 625

Query: 635 NFLCSIGYGPKMIQVITRTPVRCP-TKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTAT 694
            FLCSIGYGPK IQVITRTPVRCP T+KP P NLNYPSI AVF +  +G  +K+ IRTAT
Sbjct: 626 TFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTAT 685

Query: 695 NVGPSNSVYRAKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFG 754
           NVG + +VYRA+IE+P+GVTV VKP +LVF++ VK++S+ V ++ + +N+ LG+ GAVFG
Sbjct: 686 NVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFG 745

Query: 755 WLSWSD-GKHVVRSPLVVTQLEPL 777
            ++W D GKHVVRSP+VVTQ++ L
Sbjct: 746 SVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of PI0022653 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 1.9e-245
Identity = 452/783 (57.73%), Postives = 552/783 (70.50%), Query Frame = 0

Query: 15  FFFFFFFLLLLSTVS---SHSPLKTFIFRIDRFSKPSVFPTHYHWYTSEF----TQSPQI 74
           FFF+FFFLL LS+ S   S S   T+I  +D  +KPS+FPTH+HWYTS      +  P I
Sbjct: 3   FFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSI 62

Query: 75  LHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLW 134
           +H YDTVFHGFSA LT      +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL 
Sbjct: 63  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 122

Query: 135 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRKIVGAR 194
            +SD+GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F  + CNRK+VGAR
Sbjct: 123 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 182

Query: 195 FFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGIA 254
           FF  G+EA          +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A
Sbjct: 183 FFCGGYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 242

Query: 255 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPI 314
            G+APKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD I
Sbjct: 243 AGMAPKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAI 302

Query: 315 AIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKIY 374
           AIGA+GA  +G+FVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ I 
Sbjct: 303 AIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMIS 362

Query: 375 GVSLYAGAPLN-GTMYPLVYPGK---SGVLSVSLCMENSLDPKVVSGKIVICDRGSSPRV 434
           GVS+Y G  L+ G MYPLVY G        S SLC+E SLDP +V GKIV+CDRG + R 
Sbjct: 363 GVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRA 422

Query: 435 AKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYAS------SST 494
            KG +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS 
Sbjct: 423 TKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSK 482

Query: 495 NPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTG 554
           +PTATI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G
Sbjct: 483 HPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 542

Query: 555 LDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPM 614
           +  D R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA   DN  +PM
Sbjct: 543 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 602

Query: 615 TEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPV 674
            +ESTG  S+  D+G+GHV+   AMDPGL+YDI++ DYINFLC+  Y    I  ITR   
Sbjct: 603 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 662

Query: 675 RCPTKKPLPE--NLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVT 734
            C   +      NLNYPS   VF    +   +  FIRT TNVG S+SVY  KI  P+G T
Sbjct: 663 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 722

Query: 735 VKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQL 777
           V V+P KL F    +K SFVV +      L+ G      G + WSDGK  V SPLVVT  
Sbjct: 723 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 775

BLAST of PI0022653 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 3.9e-206
Identity = 392/773 (50.71%), Postives = 521/773 (67.40%), Query Frame = 0

Query: 9   LSPFFFFFFFFFFLLLLS---TVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTS---EFT 68
           +S  F     FF LL L      SS S   T+I  + +   PS F  H +WY S     +
Sbjct: 1   MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS 60

Query: 69  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQ 128
            S ++L+ Y+   HGFS  LTQ++ DS+   P V++V  + R +LHTTR+P FLGL  + 
Sbjct: 61  DSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHT 120

Query: 129 RGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRKI 188
             L+ ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG CE GT FTA+ CNRK+
Sbjct: 121 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 180

Query: 189 VGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYA 248
           +GARFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYA
Sbjct: 181 IGARFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 240

Query: 249 SGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYY 308
           SG A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S YY
Sbjct: 241 SGTARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYY 300

Query: 309 LDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNG 368
            D +AIGA+ A  +G+ VS SAGN GP+  S++N+APW+TTVGAGT+DR+FPA+  LGNG
Sbjct: 301 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 360

Query: 369 RKIYGVSLYAGAPLNGTMYPLVYPGK-SGVLSVSLCMENSLDPKVVSGKIVICDRGSSPR 428
           +   GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + R
Sbjct: 361 KNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINAR 420

Query: 429 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 488
           V KG VVK AGGVGMILAN  +NGE LV DAHLLPA  VG   GD ++ Y ++  NPTA+
Sbjct: 421 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 480

Query: 489 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 548
           I+  GT++G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT A GPTGL  D+
Sbjct: 481 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 540

Query: 549 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 608
           R+ EFNI+SGTSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T    +P+ + +T
Sbjct: 541 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 600

Query: 609 GKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRC-PT 668
           GKPSTP+D GAGHV+   A +PGLIYD++  DY+ FLC++ Y    I+ ++R    C P+
Sbjct: 601 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPS 660

Query: 669 KKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEA-PKGVTVKVKP 728
           K     +LNYPS    F+    G     + RT T+VG + + Y  K+ +   GV + V+P
Sbjct: 661 KSYSVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEP 720

Query: 729 SKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVT 772
           + L F    +K+S+ V  + D+   +  +    FG + WSDGKHVV SP+ ++
Sbjct: 721 AVLNFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of PI0022653 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 1.7e-204
Identity = 380/765 (49.67%), Postives = 508/765 (66.41%), Query Frame = 0

Query: 6   STSLSPFFFFFFFFFFLLLLSTVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTSEFTQSP 65
           S+S S        F FLLL +T       KT+I R++   KP  F TH+ WYTS+     
Sbjct: 4   SSSSSSSITIITTFLFLLLHTTAK-----KTYIIRVNHSDKPESFLTHHDWYTSQLNSES 63

Query: 66  QILHVYDTVFHGFSATLTQDQVDS-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 125
            +L+ Y T FHGFSA L   + DS +    S+L +FED    LHTTR+P+FLGL ++ G+
Sbjct: 64  SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV 123

Query: 126 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRKIVGA 185
                  + VIIGV DTG+ PE RSF D ++  IP +WKG CE+G+ F +  CN+K++GA
Sbjct: 124 HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGA 183

Query: 186 RFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGI 245
           R FSKG +  +       G +   E  SPRD DGHGTHT++TAAG     AS  GYA+G 
Sbjct: 184 RSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGT 243

Query: 246 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDP 305
           A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D 
Sbjct: 244 ARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRDT 303

Query: 306 IAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKI 365
           IAIGA+ A  +GVFVS SAGN GP   SV N+APWV TVGAGT+DR+FPA   LGNG+++
Sbjct: 304 IAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRL 363

Query: 366 YGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPRVAKG 425
            GVSLY+G  +      LVY  K    S +LC+  SLD  +V GKIV+CDRG + RV KG
Sbjct: 364 TGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKG 423

Query: 426 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIAFQ 485
            VV+ AGG+GMI+AN  ++GE LV D+HLLPA AVG   GD ++ Y  S + PTA + F+
Sbjct: 424 AVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFK 483

Query: 486 GTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTE 545
           GT++ +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T+
Sbjct: 484 GTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQ 543

Query: 546 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKPS 605
           FNI+SGTSM+CPH+SG A LLK+AHP+WSP+A++SA+MTTA + DN   P+ + +    S
Sbjct: 544 FNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLS 603

Query: 606 TPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYG-PKMIQVITRTPVRCPTKKPL 665
            PY  G+GHV+   A+ PGL+YDIS  +YI FLCS+ Y    ++ ++ R  V C  K   
Sbjct: 604 NPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSD 663

Query: 666 PENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSKLVF 725
           P  LNYPS    FS L  G     + R  TNVG ++SVY+  +     V + VKPSKL F
Sbjct: 664 PGQLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSF 723

Query: 726 STTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPL 769
            +  +K+ + V      + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 724 KSVGEKKRYTVTF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of PI0022653 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 2.8e-196
Identity = 379/787 (48.16%), Postives = 498/787 (63.28%), Query Frame = 0

Query: 11  PFFFFFFFFFFLLL-LSTVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTSEFTQSPQ--- 70
           PF F       + L   T +  S  KT++  +D+ + P  +  H  WY+S+     Q   
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS 69

Query: 71  ---------ILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLG 130
                    IL+ Y T FHG +A LTQ++ + + +   V+AV  + R +LHTTRSP FLG
Sbjct: 70  QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLG 129

Query: 131 LRNQRG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAT 190
           L  Q    +W++     DV++GV DTGI PE  SF+D  + P+P  W+G CETG +F   
Sbjct: 130 LERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 189

Query: 191 NCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQA 250
           NCNRKIVGAR F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A
Sbjct: 190 NCNRKIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGA 249

Query: 251 SLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDG 310
           +L G+A G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG  
Sbjct: 250 NLFGFAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG-- 309

Query: 311 VSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAV 370
             S Y  D ++I  +GA   GVFVS SAGN GP+ +S+TN++PW+TTVGA T+DR+FPA 
Sbjct: 310 -VSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPAT 369

Query: 371 VTLGNGRKIYGVSLYAGAPL--NGTMYPLVYPGK--SGVLSVSLCMENSLDPKVVSGKIV 430
           V +G  R   GVSLY G  +      YPLVY G+  S     S C++ +LD + V+GKIV
Sbjct: 370 VKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIV 429

Query: 431 ICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYA 490
           ICDRG +PRV KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA
Sbjct: 430 ICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYA 489

Query: 491 SSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAV 550
            +S   TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  +
Sbjct: 490 MTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDM 549

Query: 551 GPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNR 610
            P+ L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA + DN 
Sbjct: 550 APSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNM 609

Query: 611 RQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVIT 670
            +P+T+ S   PS+PYD GAGH++   A DPGL+YDI   +Y  FLC+    P  ++V T
Sbjct: 610 FKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFT 669

Query: 671 RTPVRC--PTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAP 730
           +   R    T    P NLNYP+I A+F   +      +  RT TNVGP  S Y+  +   
Sbjct: 670 KHSNRTCKHTLAKNPGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPF 729

Query: 731 KGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLV 777
           KG +V V+P  L F++  +K S+ V      +          FG L W    H VRSP++
Sbjct: 730 KGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVI 780

BLAST of PI0022653 vs. ExPASy TrEMBL
Match: A0A0A0LJ58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1)

HSP 1 Score: 1503.8 bits (3892), Expect = 0.0e+00
Identity = 760/776 (97.94%), Postives = 764/776 (98.45%), Query Frame = 0

Query: 1   MAAFLSTSLSPFFFFFFFFFFLLLLSTVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTSE 60
           MAAFLSTSLS      FFF FLLLLSTVSSHSPLKTFI RIDRFSKPSVFPTHYHWYTSE
Sbjct: 1   MAAFLSTSLSS-----FFFSFLLLLSTVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSE 60

Query: 61  FTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120
           FTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN
Sbjct: 61  FTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120

Query: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRK 180
           QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA NCNRK
Sbjct: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRK 180

Query: 181 IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY 240
           IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY
Sbjct: 181 IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY 240

Query: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300
           ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY
Sbjct: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300

Query: 301 YLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGN 360
           YLDPIAIG+YGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGN
Sbjct: 301 YLDPIAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGN 360

Query: 361 GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPR 420
           GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVV+GKIVICDRGSSPR
Sbjct: 361 GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPR 420

Query: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 480
           VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT
Sbjct: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 480

Query: 481 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 540
           IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFD 
Sbjct: 481 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDK 540

Query: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 600
           RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST
Sbjct: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 600

Query: 601 GKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRCPTK 660
           GKPSTPYDFGAGHVNLGLAMDPGLIYDI+NTDYINFLCSIGYGPKMIQVITRTPVRCPTK
Sbjct: 601 GKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTK 660

Query: 661 KPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSK 720
           KPLPENLNYPSIV VFSSLSKGWSTKSFIRTATNVGPSNSVYR KIEAPKGVTVKVKPSK
Sbjct: 661 KPLPENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSK 720

Query: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 777
           LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of PI0022653 vs. ExPASy TrEMBL
Match: A0A1S3C7D0 (subtilisin-like protease SBT1.6 OS=Cucumis melo OX=3656 GN=LOC103497323 PE=3 SV=1)

HSP 1 Score: 1500.0 bits (3882), Expect = 0.0e+00
Identity = 759/776 (97.81%), Postives = 763/776 (98.32%), Query Frame = 0

Query: 1   MAAFLSTSLSPFFFFFFFFFFLLLLSTVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTSE 60
           MAAFLSTSLSP     FFF FLLLLSTVSS SPLKTFI RIDRFSKPSVFPTHYHWYTSE
Sbjct: 1   MAAFLSTSLSP-----FFFSFLLLLSTVSSLSPLKTFIVRIDRFSKPSVFPTHYHWYTSE 60

Query: 61  FTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120
           FTQSPQILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN
Sbjct: 61  FTQSPQILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120

Query: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRK 180
           QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA NCNRK
Sbjct: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRK 180

Query: 181 IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY 240
           IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY
Sbjct: 181 IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY 240

Query: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300
           ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY
Sbjct: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300

Query: 301 YLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGN 360
           YLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGN
Sbjct: 301 YLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGN 360

Query: 361 GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPR 420
           GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVV+GKIVICDRGSSPR
Sbjct: 361 GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPR 420

Query: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 480
           VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT
Sbjct: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 480

Query: 481 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 540
           IAFQGTIIGIKPAPVVASFSARGPNGL PEILKPDIIAPGVNILAAWTDAVGPTGLDFDT
Sbjct: 481 IAFQGTIIGIKPAPVVASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 540

Query: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 600
           RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASI DNRRQPMTEEST
Sbjct: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEEST 600

Query: 601 GKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRCPTK 660
           GKPSTPYDFGAGHVNLGLAMDPGLIYDI+NTDYINFLCSIGYGPKMIQVITRTPVRCPTK
Sbjct: 601 GKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTK 660

Query: 661 KPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSK 720
           KPLPENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGPSNSVYR KIEAPKGVTVKVKPSK
Sbjct: 661 KPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSK 720

Query: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 777
           LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of PI0022653 vs. ExPASy TrEMBL
Match: A0A5D3BLE2 (Subtilisin-like protease SBT1.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001210 PE=3 SV=1)

HSP 1 Score: 1463.0 bits (3786), Expect = 0.0e+00
Identity = 731/741 (98.65%), Postives = 735/741 (99.19%), Query Frame = 0

Query: 36  TFIFRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPS 95
           TFI RIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQ+QVDSIGKHPS
Sbjct: 2   TFIVRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQEQVDSIGKHPS 61

Query: 96  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 155
           VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL
Sbjct: 62  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 121

Query: 156 GPIPRRWKGVCETGTKFTATNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD 215
           GPIPRRWKGVCETGTKFTA NCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD
Sbjct: 122 GPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD 181

Query: 216 ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 275
           ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF
Sbjct: 182 ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 241

Query: 276 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN 335
           DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN
Sbjct: 242 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN 301

Query: 336 LAPWVTTVGAGTIDRNFPAVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 395
           LAPWVTTVGAGTIDRNFP+VVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL
Sbjct: 302 LAPWVTTVGAGTIDRNFPSVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 361

Query: 396 CMENSLDPKVVSGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 455
           CMENSLDPKVV+GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP
Sbjct: 362 CMENSLDPKVVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 421

Query: 456 ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPD 515
           ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGL PEILKPD
Sbjct: 422 ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLTPEILKPD 481

Query: 516 IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 575
           IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA
Sbjct: 482 IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 541

Query: 576 ALRSAMMTTASITDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYIN 635
           ALRSAMMTTASI DNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDI+NTDYIN
Sbjct: 542 ALRSAMMTTASIIDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYIN 601

Query: 636 FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNV 695
           FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRT TNV
Sbjct: 602 FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNV 661

Query: 696 GPSNSVYRAKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL 755
           GPSNSVYR KIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL
Sbjct: 662 GPSNSVYRVKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL 721

Query: 756 SWSDGKHVVRSPLVVTQLEPL 777
           SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 SWSDGKHVVRSPLVVTQLEPL 742

BLAST of PI0022653 vs. ExPASy TrEMBL
Match: A0A6J1FYE5 (subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE=3 SV=1)

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 727/774 (93.93%), Postives = 751/774 (97.03%), Query Frame = 0

Query: 5   LSTSLSPFFFFFFFFFFLLL-LSTVSSHSPL-KTFIFRIDRFSKPSVFPTHYHWYTSEFT 64
           ++  LSP FFFFFFFF L++   TVS+ SPL KTFIFR+DRFSKPSVFPTHYHWYTSEF 
Sbjct: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 65  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 124
           +S +ILHVYDTVFHGFSATLTQ QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 125 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRKIV 184
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA NCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 185 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 244
           GARFFSKGHEAG+NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRH+FQASLEG+AS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 245 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 304
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 305 DPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 364
           DPIAIGAYGAAS+G+FVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 365 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPRVA 424
           +I GVSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPKVV+GKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 425 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 484
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480

Query: 485 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 544
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 545 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 604
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 605 PSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 664
            STPYDFGAGHVNLGLAMDPGL+YDI+NTDYINFLCSIGYGPKMIQVITRTPV CP KKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 665 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSKLV 724
           LPENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGP+NSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 725 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 777
           FS   KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

BLAST of PI0022653 vs. ExPASy TrEMBL
Match: A0A6J1JEF5 (subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3 SV=1)

HSP 1 Score: 1433.3 bits (3709), Expect = 0.0e+00
Identity = 719/773 (93.01%), Postives = 744/773 (96.25%), Query Frame = 0

Query: 5   LSTSLSPFFFFFFFFFFLLLLSTVSSHSPL-KTFIFRIDRFSKPSVFPTHYHWYTSEFTQ 64
           ++  LSP FFFF      L   TVS+ SPL KTFIFR+DRFSKPSVFPTHYHWYTSEF +
Sbjct: 1   MAAYLSPLFFFFLLIVHRL---TVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAE 60

Query: 65  SPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 124
           S +ILHVYDTVFHGFSATLTQ QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG
Sbjct: 61  SHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 120

Query: 125 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRKIVG 184
           LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA NCNRKIVG
Sbjct: 121 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVG 180

Query: 185 ARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASG 244
           ARFFSKGHEAG+NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRH+FQASLEG+ASG
Sbjct: 181 ARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASG 240

Query: 245 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLD 304
           IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLD
Sbjct: 241 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLD 300

Query: 305 PIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRK 364
           PIAIGAYGAAS+G+FVSSS GNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR+
Sbjct: 301 PIAIGAYGAASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRR 360

Query: 365 IYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPRVAK 424
           I GVSLYAGAPLN TM+PLVYPGKSGVLSVSLCM+NSLDPKVV+GKIVICDRGSSPRVAK
Sbjct: 361 ISGVSLYAGAPLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAK 420

Query: 425 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIAF 484
           GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIAF
Sbjct: 421 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAF 480

Query: 485 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKT 544
           QGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKT
Sbjct: 481 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT 540

Query: 545 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKP 604
           EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGK 
Sbjct: 541 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKA 600

Query: 605 STPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKPL 664
           STPYDFGAGHVNLGLAMDPGL+YDI+NTDY+NFLCSIGYGPKMIQVITRTPV CP KKPL
Sbjct: 601 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKPL 660

Query: 665 PENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSKLVF 724
           PENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGP+NSVYRAKIEAPKGVTVKVKPSKLVF
Sbjct: 661 PENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVF 720

Query: 725 STTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 777
           S  +KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 SAAMKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

BLAST of PI0022653 vs. NCBI nr
Match: XP_004147036.1 (subtilisin-like protease SBT1.6 [Cucumis sativus] >KGN61925.1 hypothetical protein Csa_006141 [Cucumis sativus])

HSP 1 Score: 1503.8 bits (3892), Expect = 0.0e+00
Identity = 760/776 (97.94%), Postives = 764/776 (98.45%), Query Frame = 0

Query: 1   MAAFLSTSLSPFFFFFFFFFFLLLLSTVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTSE 60
           MAAFLSTSLS      FFF FLLLLSTVSSHSPLKTFI RIDRFSKPSVFPTHYHWYTSE
Sbjct: 1   MAAFLSTSLSS-----FFFSFLLLLSTVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSE 60

Query: 61  FTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120
           FTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN
Sbjct: 61  FTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120

Query: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRK 180
           QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA NCNRK
Sbjct: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRK 180

Query: 181 IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY 240
           IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY
Sbjct: 181 IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY 240

Query: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300
           ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY
Sbjct: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300

Query: 301 YLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGN 360
           YLDPIAIG+YGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGN
Sbjct: 301 YLDPIAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGN 360

Query: 361 GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPR 420
           GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVV+GKIVICDRGSSPR
Sbjct: 361 GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPR 420

Query: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 480
           VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT
Sbjct: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 480

Query: 481 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 540
           IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFD 
Sbjct: 481 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDK 540

Query: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 600
           RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST
Sbjct: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 600

Query: 601 GKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRCPTK 660
           GKPSTPYDFGAGHVNLGLAMDPGLIYDI+NTDYINFLCSIGYGPKMIQVITRTPVRCPTK
Sbjct: 601 GKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTK 660

Query: 661 KPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSK 720
           KPLPENLNYPSIV VFSSLSKGWSTKSFIRTATNVGPSNSVYR KIEAPKGVTVKVKPSK
Sbjct: 661 KPLPENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSK 720

Query: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 777
           LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of PI0022653 vs. NCBI nr
Match: XP_008457681.1 (PREDICTED: subtilisin-like protease SBT1.6 [Cucumis melo])

HSP 1 Score: 1500.0 bits (3882), Expect = 0.0e+00
Identity = 759/776 (97.81%), Postives = 763/776 (98.32%), Query Frame = 0

Query: 1   MAAFLSTSLSPFFFFFFFFFFLLLLSTVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTSE 60
           MAAFLSTSLSP     FFF FLLLLSTVSS SPLKTFI RIDRFSKPSVFPTHYHWYTSE
Sbjct: 1   MAAFLSTSLSP-----FFFSFLLLLSTVSSLSPLKTFIVRIDRFSKPSVFPTHYHWYTSE 60

Query: 61  FTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120
           FTQSPQILHVYDTVFHGFSATLTQ+QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN
Sbjct: 61  FTQSPQILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120

Query: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRK 180
           QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA NCNRK
Sbjct: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRK 180

Query: 181 IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY 240
           IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY
Sbjct: 181 IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY 240

Query: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300
           ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY
Sbjct: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300

Query: 301 YLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGN 360
           YLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGN
Sbjct: 301 YLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGN 360

Query: 361 GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPR 420
           GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVV+GKIVICDRGSSPR
Sbjct: 361 GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPR 420

Query: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 480
           VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT
Sbjct: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 480

Query: 481 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 540
           IAFQGTIIGIKPAPVVASFSARGPNGL PEILKPDIIAPGVNILAAWTDAVGPTGLDFDT
Sbjct: 481 IAFQGTIIGIKPAPVVASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 540

Query: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 600
           RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASI DNRRQPMTEEST
Sbjct: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEEST 600

Query: 601 GKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRCPTK 660
           GKPSTPYDFGAGHVNLGLAMDPGLIYDI+NTDYINFLCSIGYGPKMIQVITRTPVRCPTK
Sbjct: 601 GKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTK 660

Query: 661 KPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSK 720
           KPLPENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGPSNSVYR KIEAPKGVTVKVKPSK
Sbjct: 661 KPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSK 720

Query: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 777
           LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of PI0022653 vs. NCBI nr
Match: XP_038901136.1 (subtilisin-like protease SBT1.6 [Benincasa hispida])

HSP 1 Score: 1477.2 bits (3823), Expect = 0.0e+00
Identity = 744/776 (95.88%), Postives = 756/776 (97.42%), Query Frame = 0

Query: 1   MAAFLSTSLSPFFFFFFFFFFLLLLSTVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTSE 60
           MAAFLSTSLSP    FFF   L L STVS+ SPLKTFIFR+DRFSKPSVFPTHYHWYTSE
Sbjct: 1   MAAFLSTSLSP----FFFLLLLTLHSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSE 60

Query: 61  FTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120
           F +SP+ILHVYDTVFHGFSATLTQ QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN
Sbjct: 61  FAESPKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRN 120

Query: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRK 180
           QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA NCNRK
Sbjct: 121 QRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRK 180

Query: 181 IVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGY 240
           IVGARFFSKGHEAG+NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRH+FQASLEGY
Sbjct: 181 IVGARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGY 240

Query: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300
           ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY
Sbjct: 241 ASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPY 300

Query: 301 YLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGN 360
           YLDPIAIGAYGAAS+GVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGN
Sbjct: 301 YLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGN 360

Query: 361 GRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPR 420
           GRKI GVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPK V+GKIVICDRGSSPR
Sbjct: 361 GRKISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPR 420

Query: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 480
           VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNP AT
Sbjct: 421 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPIAT 480

Query: 481 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 540
           IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT
Sbjct: 481 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 540

Query: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 600
           RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST
Sbjct: 541 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 600

Query: 601 GKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRCPTK 660
           GK STPYDFGAGHVNLGLAMDPGL+YDI+NTDYINFLCSIGYGPKMIQVITRTPV+CP K
Sbjct: 601 GKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVKCPAK 660

Query: 661 KPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSK 720
           KPLPENLNYPSIV+VFSSLSKGWSTKSFIRT TNVGPSNS YRAKIEAPKGVTVKVKPSK
Sbjct: 661 KPLPENLNYPSIVSVFSSLSKGWSTKSFIRTVTNVGPSNSAYRAKIEAPKGVTVKVKPSK 720

Query: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 777
           LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 LVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 772

BLAST of PI0022653 vs. NCBI nr
Match: KAA0045729.1 (subtilisin-like protease SBT1.6 [Cucumis melo var. makuwa] >TYJ99551.1 subtilisin-like protease SBT1.6 [Cucumis melo var. makuwa])

HSP 1 Score: 1463.0 bits (3786), Expect = 0.0e+00
Identity = 731/741 (98.65%), Postives = 735/741 (99.19%), Query Frame = 0

Query: 36  TFIFRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHPS 95
           TFI RIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQ+QVDSIGKHPS
Sbjct: 2   TFIVRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQEQVDSIGKHPS 61

Query: 96  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 155
           VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL
Sbjct: 62  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 121

Query: 156 GPIPRRWKGVCETGTKFTATNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD 215
           GPIPRRWKGVCETGTKFTA NCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD
Sbjct: 122 GPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD 181

Query: 216 ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 275
           ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF
Sbjct: 182 ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 241

Query: 276 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN 335
           DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN
Sbjct: 242 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN 301

Query: 336 LAPWVTTVGAGTIDRNFPAVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 395
           LAPWVTTVGAGTIDRNFP+VVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL
Sbjct: 302 LAPWVTTVGAGTIDRNFPSVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 361

Query: 396 CMENSLDPKVVSGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 455
           CMENSLDPKVV+GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP
Sbjct: 362 CMENSLDPKVVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 421

Query: 456 ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPD 515
           ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGL PEILKPD
Sbjct: 422 ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLTPEILKPD 481

Query: 516 IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 575
           IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA
Sbjct: 482 IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 541

Query: 576 ALRSAMMTTASITDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYIN 635
           ALRSAMMTTASI DNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDI+NTDYIN
Sbjct: 542 ALRSAMMTTASIIDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYIN 601

Query: 636 FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNV 695
           FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRT TNV
Sbjct: 602 FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNV 661

Query: 696 GPSNSVYRAKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL 755
           GPSNSVYR KIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL
Sbjct: 662 GPSNSVYRVKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL 721

Query: 756 SWSDGKHVVRSPLVVTQLEPL 777
           SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 SWSDGKHVVRSPLVVTQLEPL 742

BLAST of PI0022653 vs. NCBI nr
Match: XP_022943985.1 (subtilisin-like protease SBT1.6 [Cucurbita moschata])

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 727/774 (93.93%), Postives = 751/774 (97.03%), Query Frame = 0

Query: 5   LSTSLSPFFFFFFFFFFLLL-LSTVSSHSPL-KTFIFRIDRFSKPSVFPTHYHWYTSEFT 64
           ++  LSP FFFFFFFF L++   TVS+ SPL KTFIFR+DRFSKPSVFPTHYHWYTSEF 
Sbjct: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 65  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 124
           +S +ILHVYDTVFHGFSATLTQ QVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 125 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRKIV 184
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA NCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 185 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 244
           GARFFSKGHEAG+NAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRH+FQASLEG+AS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 245 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 304
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 305 DPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 364
           DPIAIGAYGAAS+G+FVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 365 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPRVA 424
           +I GVSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPKVV+GKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 425 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 484
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480

Query: 485 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 544
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 545 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 604
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 605 PSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 664
            STPYDFGAGHVNLGLAMDPGL+YDI+NTDYINFLCSIGYGPKMIQVITRTPV CP KKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 665 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSKLV 724
           LPENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGP+NSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 725 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 777
           FS   KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

BLAST of PI0022653 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 560/744 (75.27%), Postives = 654/744 (87.90%), Query Frame = 0

Query: 35  KTFIFRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQDQVDSIGKHP 94
           KTFIFRID  S PS+FPTHYHWY++EF +  +I+HVY TVFHGFSA +T D+ D++  HP
Sbjct: 26  KTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHP 85

Query: 95  SVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVN 154
           +VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGSDVIIGVFDTGI PERRSFSD+N
Sbjct: 86  AVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLN 145

Query: 155 LGPIPRRWKGVCETGTKFTATNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPR 214
           LGPIP+RW+GVCE+G +F+  NCNRKI+GARFF+KG +A       I GIN T+E+ SPR
Sbjct: 146 LGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAV-----IGGINKTVEFLSPR 205

Query: 215 DADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAA 274
           DADGHGTHT+STAAGRH+F+AS+ GYASG+AKGVAPKAR+A YKVCWK+SGC DSDILAA
Sbjct: 206 DADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAA 265

Query: 275 FDAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVT 334
           FDAAV DGVDVISISIGGGDG++SPYYLDPIAIG+YGAASKG+FVSSSAGN+GPNGMSVT
Sbjct: 266 FDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVT 325

Query: 335 NLAPWVTTVGAGTIDRNFPAVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVS 394
           NLAPWVTTVGA TIDRNFPA   LG+G ++ GVSLYAG PLNG M+P+VYPGKSG+ S S
Sbjct: 326 NLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS 385

Query: 395 LCMENSLDPKVVSGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLL 454
           LCMEN+LDPK V GKIVICDRGSSPRVAKGLVVKKAGGVGMILANG SNGEGLVGDAHL+
Sbjct: 386 LCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLI 445

Query: 455 PACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKP 514
           PACAVGS+EGD +KAYASS  NP A+I F+GTI+GIKPAPV+ASFS RGPNGL+PEILKP
Sbjct: 446 PACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKP 505

Query: 515 DIIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSP 574
           D+IAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSP
Sbjct: 506 DLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSP 565

Query: 575 AALRSAMMTTASITDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYI 634
           A +RSAMMTT ++ DN  + + +ESTGK +TPYD+G+GH+NLG AM+PGL+YDI+N DYI
Sbjct: 566 AVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYI 625

Query: 635 NFLCSIGYGPKMIQVITRTPVRCP-TKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTAT 694
            FLCSIGYGPK IQVITRTPVRCP T+KP P NLNYPSI AVF +  +G  +K+ IRTAT
Sbjct: 626 TFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTAT 685

Query: 695 NVGPSNSVYRAKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFG 754
           NVG + +VYRA+IE+P+GVTV VKP +LVF++ VK++S+ V ++ + +N+ LG+ GAVFG
Sbjct: 686 NVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFG 745

Query: 755 WLSWSD-GKHVVRSPLVVTQLEPL 777
            ++W D GKHVVRSP+VVTQ++ L
Sbjct: 746 SVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of PI0022653 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 850.1 bits (2195), Expect = 1.4e-246
Identity = 452/783 (57.73%), Postives = 552/783 (70.50%), Query Frame = 0

Query: 15  FFFFFFFLLLLSTVS---SHSPLKTFIFRIDRFSKPSVFPTHYHWYTSEF----TQSPQI 74
           FFF+FFFLL LS+ S   S S   T+I  +D  +KPS+FPTH+HWYTS      +  P I
Sbjct: 3   FFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSI 62

Query: 75  LHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLW 134
           +H YDTVFHGFSA LT      +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL 
Sbjct: 63  IHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLL 122

Query: 135 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRKIVGAR 194
            +SD+GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F  + CNRK+VGAR
Sbjct: 123 EESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGAR 182

Query: 195 FFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGIA 254
           FF  G+EA          +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A
Sbjct: 183 FFCGGYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 242

Query: 255 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPI 314
            G+APKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD I
Sbjct: 243 AGMAPKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAI 302

Query: 315 AIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKIY 374
           AIGA+GA  +G+FVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ I 
Sbjct: 303 AIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMIS 362

Query: 375 GVSLYAGAPLN-GTMYPLVYPGK---SGVLSVSLCMENSLDPKVVSGKIVICDRGSSPRV 434
           GVS+Y G  L+ G MYPLVY G        S SLC+E SLDP +V GKIV+CDRG + R 
Sbjct: 363 GVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRA 422

Query: 435 AKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYAS------SST 494
            KG +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS 
Sbjct: 423 TKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSK 482

Query: 495 NPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTG 554
           +PTATI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G
Sbjct: 483 HPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSG 542

Query: 555 LDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPM 614
           +  D R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA   DN  +PM
Sbjct: 543 VTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPM 602

Query: 615 TEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPV 674
            +ESTG  S+  D+G+GHV+   AMDPGL+YDI++ DYINFLC+  Y    I  ITR   
Sbjct: 603 MDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA 662

Query: 675 RCPTKKPLPE--NLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVT 734
            C   +      NLNYPS   VF    +   +  FIRT TNVG S+SVY  KI  P+G T
Sbjct: 663 DCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTT 722

Query: 735 VKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQL 777
           V V+P KL F    +K SFVV +      L+ G      G + WSDGK  V SPLVVT  
Sbjct: 723 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 775

BLAST of PI0022653 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 719.5 bits (1856), Expect = 2.8e-207
Identity = 392/773 (50.71%), Postives = 521/773 (67.40%), Query Frame = 0

Query: 9   LSPFFFFFFFFFFLLLLS---TVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTS---EFT 68
           +S  F     FF LL L      SS S   T+I  + +   PS F  H +WY S     +
Sbjct: 1   MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS 60

Query: 69  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQ 128
            S ++L+ Y+   HGFS  LTQ++ DS+   P V++V  + R +LHTTR+P FLGL  + 
Sbjct: 61  DSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHT 120

Query: 129 RGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRKI 188
             L+ ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG CE GT FTA+ CNRK+
Sbjct: 121 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 180

Query: 189 VGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYA 248
           +GARFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYA
Sbjct: 181 IGARFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 240

Query: 249 SGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYY 308
           SG A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S YY
Sbjct: 241 SGTARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYY 300

Query: 309 LDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNG 368
            D +AIGA+ A  +G+ VS SAGN GP+  S++N+APW+TTVGAGT+DR+FPA+  LGNG
Sbjct: 301 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 360

Query: 369 RKIYGVSLYAGAPLNGTMYPLVYPGK-SGVLSVSLCMENSLDPKVVSGKIVICDRGSSPR 428
           +   GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + R
Sbjct: 361 KNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINAR 420

Query: 429 VAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTAT 488
           V KG VVK AGGVGMILAN  +NGE LV DAHLLPA  VG   GD ++ Y ++  NPTA+
Sbjct: 421 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 480

Query: 489 IAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDT 548
           I+  GT++G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT A GPTGL  D+
Sbjct: 481 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 540

Query: 549 RKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEEST 608
           R+ EFNI+SGTSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T    +P+ + +T
Sbjct: 541 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 600

Query: 609 GKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVITRTPVRC-PT 668
           GKPSTP+D GAGHV+   A +PGLIYD++  DY+ FLC++ Y    I+ ++R    C P+
Sbjct: 601 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPS 660

Query: 669 KKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEA-PKGVTVKVKP 728
           K     +LNYPS    F+    G     + RT T+VG + + Y  K+ +   GV + V+P
Sbjct: 661 KSYSVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEP 720

Query: 729 SKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVT 772
           + L F    +K+S+ V  + D+   +  +    FG + WSDGKHVV SP+ ++
Sbjct: 721 AVLNFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of PI0022653 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 714.1 bits (1842), Expect = 1.2e-205
Identity = 380/765 (49.67%), Postives = 508/765 (66.41%), Query Frame = 0

Query: 6   STSLSPFFFFFFFFFFLLLLSTVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTSEFTQSP 65
           S+S S        F FLLL +T       KT+I R++   KP  F TH+ WYTS+     
Sbjct: 4   SSSSSSSITIITTFLFLLLHTTAK-----KTYIIRVNHSDKPESFLTHHDWYTSQLNSES 63

Query: 66  QILHVYDTVFHGFSATLTQDQVDS-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 125
            +L+ Y T FHGFSA L   + DS +    S+L +FED    LHTTR+P+FLGL ++ G+
Sbjct: 64  SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV 123

Query: 126 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTATNCNRKIVGA 185
                  + VIIGV DTG+ PE RSF D ++  IP +WKG CE+G+ F +  CN+K++GA
Sbjct: 124 HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGA 183

Query: 186 RFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGI 245
           R FSKG +  +       G +   E  SPRD DGHGTHT++TAAG     AS  GYA+G 
Sbjct: 184 RSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGT 243

Query: 246 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDP 305
           A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D 
Sbjct: 244 ARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRDT 303

Query: 306 IAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKI 365
           IAIGA+ A  +GVFVS SAGN GP   SV N+APWV TVGAGT+DR+FPA   LGNG+++
Sbjct: 304 IAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRL 363

Query: 366 YGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVSGKIVICDRGSSPRVAKG 425
            GVSLY+G  +      LVY  K    S +LC+  SLD  +V GKIV+CDRG + RV KG
Sbjct: 364 TGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKG 423

Query: 426 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIAFQ 485
            VV+ AGG+GMI+AN  ++GE LV D+HLLPA AVG   GD ++ Y  S + PTA + F+
Sbjct: 424 AVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFK 483

Query: 486 GTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTE 545
           GT++ +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T+
Sbjct: 484 GTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQ 543

Query: 546 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKPS 605
           FNI+SGTSM+CPH+SG A LLK+AHP+WSP+A++SA+MTTA + DN   P+ + +    S
Sbjct: 544 FNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLS 603

Query: 606 TPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYG-PKMIQVITRTPVRCPTKKPL 665
            PY  G+GHV+   A+ PGL+YDIS  +YI FLCS+ Y    ++ ++ R  V C  K   
Sbjct: 604 NPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSD 663

Query: 666 PENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAPKGVTVKVKPSKLVF 725
           P  LNYPS    FS L  G     + R  TNVG ++SVY+  +     V + VKPSKL F
Sbjct: 664 PGQLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSF 723

Query: 726 STTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPL 769
            +  +K+ + V      + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 724 KSVGEKKRYTVTF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of PI0022653 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 686.8 bits (1771), Expect = 2.0e-197
Identity = 379/787 (48.16%), Postives = 498/787 (63.28%), Query Frame = 0

Query: 11  PFFFFFFFFFFLLL-LSTVSSHSPLKTFIFRIDRFSKPSVFPTHYHWYTSEFTQSPQ--- 70
           PF F       + L   T +  S  KT++  +D+ + P  +  H  WY+S+     Q   
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS 69

Query: 71  ---------ILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLG 130
                    IL+ Y T FHG +A LTQ++ + + +   V+AV  + R +LHTTRSP FLG
Sbjct: 70  QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLG 129

Query: 131 LRNQRG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAT 190
           L  Q    +W++     DV++GV DTGI PE  SF+D  + P+P  W+G CETG +F   
Sbjct: 130 LERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 189

Query: 191 NCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQA 250
           NCNRKIVGAR F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A
Sbjct: 190 NCNRKIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGA 249

Query: 251 SLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDG 310
           +L G+A G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG  
Sbjct: 250 NLFGFAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG-- 309

Query: 311 VSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAV 370
             S Y  D ++I  +GA   GVFVS SAGN GP+ +S+TN++PW+TTVGA T+DR+FPA 
Sbjct: 310 -VSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPAT 369

Query: 371 VTLGNGRKIYGVSLYAGAPL--NGTMYPLVYPGK--SGVLSVSLCMENSLDPKVVSGKIV 430
           V +G  R   GVSLY G  +      YPLVY G+  S     S C++ +LD + V+GKIV
Sbjct: 370 VKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIV 429

Query: 431 ICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYA 490
           ICDRG +PRV KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA
Sbjct: 430 ICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYA 489

Query: 491 SSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAV 550
            +S   TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  +
Sbjct: 490 MTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDM 549

Query: 551 GPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNR 610
            P+ L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA + DN 
Sbjct: 550 APSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNM 609

Query: 611 RQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDISNTDYINFLCSIGYGPKMIQVIT 670
            +P+T+ S   PS+PYD GAGH++   A DPGL+YDI   +Y  FLC+    P  ++V T
Sbjct: 610 FKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFT 669

Query: 671 RTPVRC--PTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPSNSVYRAKIEAP 730
           +   R    T    P NLNYP+I A+F   +      +  RT TNVGP  S Y+  +   
Sbjct: 670 KHSNRTCKHTLAKNPGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPF 729

Query: 731 KGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLV 777
           KG +V V+P  L F++  +K S+ V      +          FG L W    H VRSP++
Sbjct: 730 KGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVI 780