PI0022456 (gene) Melon (PI 482460) v1

Overview


Name	PI0022456
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	cysteine proteinase inhibitor 5-like
Location	chr01: 1544401 .. 1545111 (-)
RNA-Seq Expression	PI0022456
Synteny	PI0022456

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCAATAGGTATCAATCTCACAAGGCTCTAGAACTACACCATTTAGAAGATATCACATTATTGATACACTTCTATAAATATCAAACAGCTACAAGAACCAAAATCATCACAAACTAAACCTTTTGTCCATATACACCAATACTAAATCAAATTAGGTCACAATGATTTCTACTGTGCTTGCCAACATCGGTCAACATGATCCCATCCTCCACGTGACAAAGGATACAAAGGGCGATCATCAATCCGAATGGATATCGATTCGCGCTATCGAAGACCCTTACGTGCAAGAAGTCGGGAAATTCGCCGTGAGAGAATTCGAGAAGGAAAAAATGGCAAGGTTTCAATACAAACGCTTGCTTAGTTGTTGGATTAAAGAGGAGATGGGAGGCATAAAATATCGCCTTATTTTAGAGGTAATTAAAGAGGAAAAGATTGAGGGTAAATATGAAGCTATTGTATGGGAGGCTTCTGGCCGTTTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTGTATGCATGGGGCTGCATGACATTTCCCCTCTTTGTATTATTCAATCTACAAAGCTTATGGTGTGAAATGCCATTGGTAAAATAAAAAAGTATATATATACTTTCTATGTAAAAATCTTCTCTATGTTCAAAAACTAACTTTTACCATATGGGTAAATTAATTATTACACCTATGGATCTTTGTGTTCAATCAAA

mRNA sequence

CCCAATAGGTATCAATCTCACAAGGCTCTAGAACTACACCATTTAGAAGATATCACATTATTGATACACTTCTATAAATATCAAACAGCTACAAGAACCAAAATCATCACAAACTAAACCTTTTGTCCATATACACCAATACTAAATCAAATTAGGTCACAATGATTTCTACTGTGCTTGCCAACATCGGTCAACATGATCCCATCCTCCACGTGACAAAGGATACAAAGGGCGATCATCAATCCGAATGGATATCGATTCGCGCTATCGAAGACCCTTACGTGCAAGAAGTCGGGAAATTCGCCGTGAGAGAATTCGAGAAGGAAAAAATGGCAAGGTTTCAATACAAACGCTTGCTTAGTTGTTGGATTAAAGAGGAGATGGGAGGCATAAAATATCGCCTTATTTTAGAGGTAATTAAAGAGGAAAAGATTGAGGGTAAATATGAAGCTATTGTATGGGAGGCTTCTGGCCGTTTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTGTATGCATGGGGCTGCATGACATTTCCCCTCTTTGTATTATTCAATCTACAAAGCTTATGGTGTGAAATGCCATTGGTAAAATAAAAAAGTATATATATACTTTCTATGTAAAAATCTTCTCTATGTTCAAAAACTAACTTTTACCATATGGGTAAATTAATTATTACACCTATGGATCTTTGTGTTCAATCAAA

Coding sequence (CDS)

ATGATTTCTACTGTGCTTGCCAACATCGGTCAACATGATCCCATCCTCCACGTGACAAAGGATACAAAGGGCGATCATCAATCCGAATGGATATCGATTCGCGCTATCGAAGACCCTTACGTGCAAGAAGTCGGGAAATTCGCCGTGAGAGAATTCGAGAAGGAAAAAATGGCAAGGTTTCAATACAAACGCTTGCTTAGTTGTTGGATTAAAGAGGAGATGGGAGGCATAAAATATCGCCTTATTTTAGAGGTAATTAAAGAGGAAAAGATTGAGGGTAAATATGAAGCTATTGTATGGGAGGCTTCTGGCCGTTTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTGTATGCATGGGGCTGCATGA

Protein sequence

MISTVLANIGQHDPILHVTKDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMARFQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPCMHGAA

Homology

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BLAST of PI0022456 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 2.8e-05
Identity = 31/83 (37.35%), Postives = 47/83 (56.63%), Query Frame = 0


Query: 36  IEDPYVQEVGKFAVREFEKEKMARFQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKY 95
           I DP+VQE+G++AV E ++   A       ++   K+ + G+ YRL LE       +G+Y
Sbjct: 35  INDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDGRY 94

Query: 96  EAIVWEASGRLIIRKLKLISFMP 119
           EA+V+E   R   R  KL+SF P
Sbjct: 95  EAVVYEQDPRSNAR--KLVSFEP 115

[Close]

BLAST of PI0022456 vs. ExPASy TrEMBL
Match: A0A5A7SN35 (Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001920 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.4e-60
Identity = 121/125 (96.80%), Postives = 121/125 (96.80%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVT-KDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMAR 60
           MISTVLANIGQHDPILHVT KDTKGDHQSEWISIRAIEDPYVQEV KFAVREFEKEKMAR
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  FQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120
            QYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC
Sbjct: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120

Query: 121 MHGAA 125
           MHG A
Sbjct: 121 MHGVA 125

[Close]

BLAST of PI0022456 vs. ExPASy TrEMBL
Match: A0A0A0K644 (Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.7e-59
Identity = 116/125 (92.80%), Postives = 122/125 (97.60%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVT-KDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMAR 60
           MIST+LAN+GQHDPILHVT KDTKGDHQSEWISIRAIEDPYVQEV KFA+REFEKEKMAR
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120
           FQYKRLLSCWIKEEMGGIKYRL+LEVIKEEK+EGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 MHGAA 125
           MHGAA
Sbjct: 121 MHGAA 125

[Close]

BLAST of PI0022456 vs. ExPASy TrEMBL
Match: A0A6J1IC35 (cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 4.8e-08
Identity = 37/105 (35.24%), Postives = 61/105 (58.10%), Query Frame = 0


Query: 14  PILHVTKDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMARFQYKRLLSCWIKEE 73
           P+L V     G     W  I+ ++DP++QE+GKFAV E+ K+     ++K ++     + 
Sbjct: 15  PLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQV 74

Query: 74  MGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMP 119
           + G+ YRL++E  K E I GKY+A+VWE + +     ++L SF P
Sbjct: 75  VSGMNYRLVIEAKKGESI-GKYQALVWEKAWQ---HFMELTSFKP 114

[Close]

BLAST of PI0022456 vs. ExPASy TrEMBL
Match: A0A6J1EKC1 (cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111434113 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 6.3e-08
Identity = 36/105 (34.29%), Postives = 61/105 (58.10%), Query Frame = 0


Query: 14  PILHVTKDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMARFQYKRLLSCWIKEE 73
           P+L V     G     W  I+ ++DP++QE+GKFAV E+ K+     ++K ++     + 
Sbjct: 15  PLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQV 74

Query: 74  MGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMP 119
           + G+ YRL++E  K E I GKY+A++WE + +     ++L SF P
Sbjct: 75  VSGMNYRLVIEAKKGESI-GKYQALIWEKAWQ---HFMELTSFKP 114

[Close]

BLAST of PI0022456 vs. ExPASy TrEMBL
Match: A0A6J1IK23 (cysteine proteinase inhibitor A-like OS=Cucurbita maxima OX=3661 GN=LOC111475738 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.0e-06
Identity = 42/111 (37.84%), Postives = 57/111 (51.35%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVTKDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMARF 60
           M S V A  GQ++ +    +D KG    +W  I  I DPYVQE+G+FAV E  K      
Sbjct: 1   MSSYVEAAPGQNEAVF---QDQKGIECGQWEPIVNIMDPYVQELGRFAVMENNKNTRGHL 60

Query: 61  QYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIV----WEASGRLI 108
           ++  ++  W  + + G  YRLI+E   E    G YEAIV    WE +  LI
Sbjct: 61  KFICVIKGW-TQVVAGKNYRLIIEACNELGFPGTYEAIVYVKPWEKTWELI 107

[Close]

BLAST of PI0022456 vs. NCBI nr
Match: KAA0031436.1 (Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystatin domain containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 241.1 bits (614), Expect = 5.0e-60
Identity = 121/125 (96.80%), Postives = 121/125 (96.80%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVT-KDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMAR 60
           MISTVLANIGQHDPILHVT KDTKGDHQSEWISIRAIEDPYVQEV KFAVREFEKEKMAR
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  FQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120
            QYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC
Sbjct: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120

Query: 121 MHGAA 125
           MHG A
Sbjct: 121 MHGVA 125

[Close]

BLAST of PI0022456 vs. NCBI nr
Match: KGN43767.1 (hypothetical protein Csa_017208 [Cucumis sativus])

HSP 1 Score: 237.7 bits (605), Expect = 5.5e-59
Identity = 116/125 (92.80%), Postives = 122/125 (97.60%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVT-KDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMAR 60
           MIST+LAN+GQHDPILHVT KDTKGDHQSEWISIRAIEDPYVQEV KFA+REFEKEKMAR
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120
           FQYKRLLSCWIKEEMGGIKYRL+LEVIKEEK+EGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 MHGAA 125
           MHGAA
Sbjct: 121 MHGAA 125

[Close]

BLAST of PI0022456 vs. NCBI nr
Match: KAG6571859.1 (hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011544.1 hypothetical protein SDJN02_26450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 191.4 bits (485), Expect = 4.6e-45
Identity = 96/118 (81.36%), Postives = 100/118 (84.75%), Query Frame = 0


Query: 5   VLANIGQHDPILHVTKDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMARFQYKR 64
           +LANIGQHDPILHVTKD KGDH SEWISIRAIEDPYVQEV KF V E  KEK A FQYK 
Sbjct: 1   MLANIGQHDPILHVTKDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYKG 60

Query: 65  LLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPCMHG 123
           LL CW+KEEMGGIKYRLILEV K    EGKYEAIVWEASGRLIIRKLKL+SF+ C HG
Sbjct: 61  LLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHG 117

[Close]

BLAST of PI0022456 vs. NCBI nr
Match: KAG6588760.1 (hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 189.9 bits (481), Expect = 1.3e-44
Identity = 93/121 (76.86%), Postives = 103/121 (85.12%), Query Frame = 0


Query: 1   MISTVLANIGQHDPILHVTKDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMARF 60
           MIST+L NIGQHDPILHVTKD KGD +SEWISIR IEDPYVQEV KFAV EF  +KM  F
Sbjct: 1   MISTLLTNIGQHDPILHVTKDVKGDPRSEWISIRTIEDPYVQEVAKFAVIEFNVDKMTTF 60

Query: 61  QYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEG--KYEAIVWEASGRLIIRKLKLISFMP 120
           QYKR+L CWIKEEMGGIKYRL+LEV +E   +   KYEA+VWEASGRLIIRKLKL+SF+P
Sbjct: 61  QYKRMLRCWIKEEMGGIKYRLVLEVKQEAPTQKVCKYEAVVWEASGRLIIRKLKLVSFVP 120

[Close]

BLAST of PI0022456 vs. NCBI nr
Match: XP_022973098.1 (cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine proteinase inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 67.4 bits (163), Expect = 9.9e-08
Identity = 37/105 (35.24%), Postives = 61/105 (58.10%), Query Frame = 0


Query: 14  PILHVTKDTKGDHQSEWISIRAIEDPYVQEVGKFAVREFEKEKMARFQYKRLLSCWIKEE 73
           P+L V     G     W  I+ ++DP++QE+GKFAV E+ K+     ++K ++     + 
Sbjct: 15  PLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQV 74

Query: 74  MGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMP 119
           + G+ YRL++E  K E I GKY+A+VWE + +     ++L SF P
Sbjct: 75  VSGMNYRLVIEAKKGESI-GKYQALVWEKAWQ---HFMELTSFKP 114

[Close]

BLAST of PI0022456 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 3.2e-04
Identity = 27/93 (29.03%), Postives = 47/93 (50.54%), Query Frame = 0


Query: 30  WISIRAIEDPYVQEVGKFAVREFEKEKMARFQYKRLLSCWIKEEMGGIKYRLILEVIKEE 89
           W  I  + DP V E+G+FAV E+ K   +  +++ ++S    + + G  YRL +     +
Sbjct: 31  WSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVS-GETQVVSGTNYRLKVAANDGD 90

Query: 90  KIEGKYEAIVWEASGRLIIRKLKLISFMPCMHG 123
            +   Y AIVW+   +  ++   L SF P  +G
Sbjct: 91  GVSKNYLAIVWD---KPWMKFRNLTSFEPANNG 119

The following BLAST results are available for this feature:

BLAST of PI0022456 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10Q47	2.8e-05	37.35	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]

BLAST of PI0022456 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SN35	2.4e-60	96.80	Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0K644	2.7e-59	92.80	Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE...	[more]
A0A6J1IC35	4.8e-08	35.24	cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628...	[more]
A0A6J1EKC1	6.3e-08	34.29	cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC1114341...	[more]
A0A6J1IK23	2.0e-06	37.84	cysteine proteinase inhibitor A-like OS=Cucurbita maxima OX=3661 GN=LOC111475738...	[more]

BLAST of PI0022456 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0031436.1	5.0e-60	96.80	Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystat...	[more]
KGN43767.1	5.5e-59	92.80	hypothetical protein Csa_017208 [Cucumis sativus]	[more]
KAG6571859.1	4.6e-45	81.36	hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6588760.1	1.3e-44	76.86	hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022973098.1	9.9e-08	35.24	cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine p...	[more]

BLAST of PI0022456 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	3.2e-04	29.03	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 28..123 e-value: 3.4E-15 score: 57.9
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 27..117
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 30..119
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 33..104 e-value: 6.4E-12 score: 45.6
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 27..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0022456.1	PI0022456.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity