PI0022374 (gene) Melon (PI 482460) v1

Overview
NamePI0022374
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
Locationchr01: 23105054 .. 23108321 (-)
RNA-Seq ExpressionPI0022374
SyntenyPI0022374
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAAGCATCCAAAGAAGAGCCAAAGGAGATGATGATGAACTCTCCTTCAAGGCTGTTTAAGAGGAAAAAATGGACAAAGTTTGATAAAAACGTTGCCTTTTACTTTCTTCTTTTGCATCTACTTTGTATTTTGGCACCATTATATTTTAGTTGGACTTGTTTGTTGCTTTCTTTGGGATTAGCCAATCTAACAGGTATGGCAATTAGTGTTTCGTATCATAGAAATCTTGCACATCGAAGCTTCAAACTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAGCTCATGCAATTCAGGTTCGATATTTTGAATCCCTTGCTCTAACTAAAAATGGACTAAACTATGAATAAAAAATGAATAACAAATACTTTTCGATTTTATATTAATTTAAGGTTGAACACTATAGATGACACAATATTTAAATTTCACTCCACCCATTAACTTAAACTTTTGAATTAATCAATGATTTAATATGATATTAAACTAAATGGTTCATGAGGTTCTTCTGTATTTGAACACATGCAATGTTACTACCTCCTCAATTAATATCGATTCTCACTTTTTGGGTCTTGTACAGACATTTCAATCCCACAAATGTTAGTGTTAAATGATTTTGAATCAACTGATAATTTCACGATGTATTATTTTTTGTTTTTTTAATCTAAAAACAAACATTATTGATCATTACGTTATATATGAAAAATGATTTTTATACCCATTACTTAAACACCAAATCATAAAATTAGAATGAGTGGTGGAGTAATTGCCTACATGCCATTAAAAAGATAAAATTAAAGACAAACCCTATATCATATTTTAAATATGGTCTTTTGATAACATTTTGTTGGTTCTTCAATTTTAGGGTGATCCAATTGATTGGGTGAGCACACACAGATGTCACCATCGATTTGTTGATTCAGAACGAGATCCACATAGTCCTATTTATGGATTCTGGTTTAGTCAAATGACGTGGTTTTTCGATTCGTATAACTTGACGAGAAAAGTTTGTCCTAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATCTTCATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAGGTTCTATAGATTTATACACCAAACATATTGGCTTCATCCACTTGCTCTTTCATTCTTGCTCTATGCCATTGGAGGACTTCCTTTCATCATTTGGGGAATGGTAAGATATAGTTATTATTCCCTTTCCCTTTCCTCTTTTAATTATTAAGTTATGAAATCTATTTGTTTGGATGTTTAAATTCTTTTCAATTGTTAATTTTTCTTTACAAATTTTTAAAAAAGAAGGAAATTTTAATTTTTTTCATTAATAAATTAAGTTTTTGATTTTTTTACATTGTTATATTTAATTTATTCACTACATGTTTTTAACATCTACACAGTAGATAATTTAAGGGTTTTTAAAAAAGAAAATAAAAAAGAAAGAAAAAGAAAATGCATGCATGTATAATGCTTTTTTTTTTTCAATAAAGGTATTTGTTGTATTTTTATTTTATTTTATTTGACAATTAATCATTAATTTATATTATATATAAAGTAAAATCAATATTATATATAAGACAGTGAACCCTAAACTCTAAATCCTAAATCTTAAACCAGTTAAATTACAAGTTTAACTCTTTATTGAGGTGCAAAACTTATGTCCACCTAATTCTTGATCTCTTAAAAATATGTAGTAGGTTTCTAGACTGTTTGTATGTTCAACCAAGCCGTACTTTCAATTTTTTTTGTTTTGAAGATCTGTGAATTTGGAAAGAAATTAAAATTTTCTTATCTTGTTTGATGATGATTTCATTTTAATTTTATGTAAATTTTTTTGCTTGTTTCCTCTCAATTTTCTTACCATGATTTTTATCTTTTAAAAAAAACATTAAGATTTTTCGTCTAACCAAAAACATAAATTACAAAATCTTTAAAACCGCTTTTTTTTTTTTTTTTCTCTATTTTGTTGAAACACTAGATGATTGATAATATCAATCTAAAGTATTGTCTTTGATTATGATTATAATAATGAAATTAAGCTAGAGAGATTGATGATATATTTCTACCATTTATTTATTTAATTATATATTTTATTGATGAAAGTGTTTTTGTGTTTGATTAAACAATTGATAGTGCGTGAGGTTCGTACTCAGCTTGCATTTCACATCTATGATAAATTCAGTTTGTCATATTTGGGGAAACCAACCATGGAACACTGGAGATTTATCTAAGAACAATTGGTAAGACATACCCATTTATCTATCTGTCTTTTTATTTATATTAATTAATGATTCATACATCAATTTTCATATTTACATCATCAATCCAATTATATAAATTTTTAAATATTCTATATCAATATCAACCCATTACCTTTTGTTTTTAACTTACCAAGTAAATTTTTAAAACCTTATTAGTGACAATTACAAAAGTGATGTTTATGTAAAATTTAGAGATCAAAGATCTGTTTCAAATAAAAAATATATATATTTTCCATATAAATTCTATTATATATTACTTGTGATTATTTTATTGGTTTGAATATGTTTTTATTTATTTCAAATCACATATTTTGAAGATTTTCAAATAATCTCTTGGATAATCTATCACTTACTGTTTTTTTTACAAGATTCTTAAATTAACCCCTTTTTTTTAAAAAAAAAAAAAAAAACTATTTCTTATTTAGTATGATGAAAAAGAACACTTTTATCATATAAAATATTCAAATAATTCATACCACATGCCTACTTTAGATTATTAATTTTTTCAACTGCAGAAAAAACCAATTTTATTATTAAAAACTATTTTTTCCAATATAGTTAGGTATGTAATAGAATGTCACTATCTATCGGTAATAGACAAAGATGGACACCGGTAAAGAAAAATATCAATTGGTGTCTAAATGTTCCTGTCTATCAAAGTTATAGACAATGATAGACATCTATCAGTATCTAGATGCCCTTTGACAAATTTTAAATAGTTCATTGTTTTATTTTTAGGATGGTTGCTATATTTACTTTTGGAGAAGGATGGCACAATAACCACCATGCTTTCGAGTATTCGGCACGACATGGTCATGAATGGTGGCAAATTGATTATGGTTGGTATATTATTAAGTTCCTTCAAGTCATTGGATTAGCCACCAATGTTAAATTACCTTCTGGAAAGCAAAAATTGGCTGCTTTAAACAAATCAAATCCCACCATTCTTAAGCCTATGATTGATTGA

mRNA sequence

ATGGAAGAAGCATCCAAAGAAGAGCCAAAGGAGATGATGATGAACTCTCCTTCAAGGCTGTTTAAGAGGAAAAAATGGACAAAGTTTGATAAAAACGTTGCCTTTTACTTTCTTCTTTTGCATCTACTTTGTATTTTGGCACCATTATATTTTAGTTGGACTTGTTTGTTGCTTTCTTTGGGATTAGCCAATCTAACAGGTATGGCAATTAGTGTTTCGTATCATAGAAATCTTGCACATCGAAGCTTCAAACTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAGCTCATGCAATTCAGGGTGATCCAATTGATTGGGTGAGCACACACAGATGTCACCATCGATTTGTTGATTCAGAACGAGATCCACATAGTCCTATTTATGGATTCTGGTTTAGTCAAATGACGTGGTTTTTCGATTCGTATAACTTGACGAGAAAAGTTTGTCCTAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATCTTCATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAGGTTCTATAGATTTATACACCAAACATATTGGCTTCATCCACTTGCTCTTTCATTCTTGCTCTATGCCATTGGAGGACTTCCTTTCATCATTTGGGGAATGTGCGTGAGGTTCGTACTCAGCTTGCATTTCACATCTATGATAAATTCAGTTTGTCATATTTGGGGAAACCAACCATGGAACACTGGAGATTTATCTAAGAACAATTGGATGGTTGCTATATTTACTTTTGGAGAAGGATGGCACAATAACCACCATGCTTTCGAGTATTCGGCACGACATGGTCATGAATGGTGGCAAATTGATTATGGTTGGTATATTATTAAGTTCCTTCAAGTCATTGGATTAGCCACCAATGTTAAATTACCTTCTGGAAAGCAAAAATTGGCTGCTTTAAACAAATCAAATCCCACCATTCTTAAGCCTATGATTGATTGA

Coding sequence (CDS)

ATGGAAGAAGCATCCAAAGAAGAGCCAAAGGAGATGATGATGAACTCTCCTTCAAGGCTGTTTAAGAGGAAAAAATGGACAAAGTTTGATAAAAACGTTGCCTTTTACTTTCTTCTTTTGCATCTACTTTGTATTTTGGCACCATTATATTTTAGTTGGACTTGTTTGTTGCTTTCTTTGGGATTAGCCAATCTAACAGGTATGGCAATTAGTGTTTCGTATCATAGAAATCTTGCACATCGAAGCTTCAAACTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAGCTCATGCAATTCAGGGTGATCCAATTGATTGGGTGAGCACACACAGATGTCACCATCGATTTGTTGATTCAGAACGAGATCCACATAGTCCTATTTATGGATTCTGGTTTAGTCAAATGACGTGGTTTTTCGATTCGTATAACTTGACGAGAAAAGTTTGTCCTAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATCTTCATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAGGTTCTATAGATTTATACACCAAACATATTGGCTTCATCCACTTGCTCTTTCATTCTTGCTCTATGCCATTGGAGGACTTCCTTTCATCATTTGGGGAATGTGCGTGAGGTTCGTACTCAGCTTGCATTTCACATCTATGATAAATTCAGTTTGTCATATTTGGGGAAACCAACCATGGAACACTGGAGATTTATCTAAGAACAATTGGATGGTTGCTATATTTACTTTTGGAGAAGGATGGCACAATAACCACCATGCTTTCGAGTATTCGGCACGACATGGTCATGAATGGTGGCAAATTGATTATGGTTGGTATATTATTAAGTTCCTTCAAGTCATTGGATTAGCCACCAATGTTAAATTACCTTCTGGAAAGCAAAAATTGGCTGCTTTAAACAAATCAAATCCCACCATTCTTAAGCCTATGATTGATTGA

Protein sequence

MEEASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLATNVKLPSGKQKLAALNKSNPTILKPMID
Homology
BLAST of PI0022374 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 355.9 bits (912), Expect = 4.9e-97
Identity = 165/290 (56.90%), Postives = 203/290 (70.00%), Query Frame = 0

Query: 24  KKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGLANLTG-MAISVSYHRNLAHRS 83
           ++W   D       L +HLL +LAP  F+W  + ++ GL  +TG + I++S+HRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 84  FKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMTWFFD 143
           FKLPKWLEY  AY  A A+QG+PIDWVSTHR HH+F DS+RDPHSP+ GFWFS M W FD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 144 SYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQRFYRFIHQTYWLHPLALS 203
           +  +T++                       G P+NV DLEKQ FYRF+  TY LHPLAL+
Sbjct: 216 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 275

Query: 204 FLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLSKNNWMVAIFTFG 263
             LYA+GG PFI+WGM VR V   H T ++NS CH+WG Q WNTGDLSKNNW VA   FG
Sbjct: 276 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFG 335

Query: 264 EGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLATNVKLPSGKQK 313
           EGWHNNHHAFE+SARHG EWWQ+D  WY++KFLQ IGLAT+VKLPS  QK
Sbjct: 336 EGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQK 363

BLAST of PI0022374 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 322.8 bits (826), Expect = 4.6e-87
Identity = 151/301 (50.17%), Postives = 193/301 (64.12%), Query Frame = 0

Query: 11  EMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGLANLTGMAI 70
           E+++    R F  + WT +D +    F+  HLL +LAP YFSW    +   L  + G+ I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 71  SVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 130
           ++SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  +++RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 131 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQRFYRFI 190
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRF+
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 191 HQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLS 250
            +T  LH +A SFL Y  GG+P ++WG+ +   + LH T ++NSVCHIWG + WNT D S
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 251 KNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLATNVKLPSGK 310
           KNNW VAI T GEGWHNNHHAFE+SARHG EWWQ+D  W +I+FL+ IGLATNVKLP+  
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 311 Q 312
           Q
Sbjct: 376 Q 354

BLAST of PI0022374 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.7e-86
Identity = 156/318 (49.06%), Postives = 200/318 (62.89%), Query Frame = 0

Query: 1   MEEASKEEPKEMM------MNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWT 60
           +  + KEE  + M      M    R    +KW + D   AF  L +H LC+LAP  F+W 
Sbjct: 3   LSASEKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWP 62

Query: 61  CLLLSLGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRC 120
            L ++L +  + G+ I+VSYHRNLAHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR 
Sbjct: 63  ALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRY 122

Query: 121 HHRFVDSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGY 180
           HH+F DS+RDPHSP  GFWFS + W FD+  L  K                       G 
Sbjct: 123 HHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GR 182

Query: 181 PDNVTDLEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINS 240
             NV DL++Q +Y+F+ +T   H L   FLLY  GGL F+ WGM +   +  H T +INS
Sbjct: 183 RTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINS 242

Query: 241 VCHIWGNQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKF 300
           +CH+WG++ W T D S+N W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++F
Sbjct: 243 LCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRF 298

Query: 301 LQVIGLATNVKLPSGKQK 313
           L++IGLAT+VKLPS  Q+
Sbjct: 303 LEIIGLATDVKLPSESQR 298

BLAST of PI0022374 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 315.1 bits (806), Expect = 9.5e-85
Identity = 154/309 (49.84%), Postives = 198/309 (64.08%), Query Frame = 0

Query: 4   ASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGLA 63
           A+ EE K+       R ++R  + KF  +       +H L +LAP YF+W+ L ++    
Sbjct: 19  AAVEEKKKRRWVFWDRRWRRLDYVKFSAS-----FTVHSLALLAPFYFTWSALWVTFLFY 78

Query: 64  NLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSER 123
            + G+ I+VSYHRNLAHRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DSER
Sbjct: 79  TIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSER 138

Query: 124 DPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEK 183
           DPHSP  GFWFS + W +DS  L  K                       G   NV DL++
Sbjct: 139 DPHSPKEGFWFSHLLWIYDSAYLVSKC----------------------GRRANVEDLKR 198

Query: 184 QRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQP 243
           Q FYRF+ +T   H L L F L+ +GG+ F+ WGM V   L +H T +INS+CHIWG + 
Sbjct: 199 QWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRT 258

Query: 244 WNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLATN 303
           W T D S+N W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++F ++IGLAT+
Sbjct: 259 WKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATD 300

Query: 304 VKLPSGKQK 313
           VK+P+  Q+
Sbjct: 319 VKVPTEAQR 300

BLAST of PI0022374 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.7e-84
Identity = 150/297 (50.51%), Postives = 191/297 (64.31%), Query Frame = 0

Query: 24  KKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGLANLTGM-AISVSYHRNLAHRS 83
           +KW   D     +   LH+LC+ AP  FSW    +   L  + G+   ++S+HRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 84  FKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMTWFFD 143
           FKLPK+LEY  AY+  HA+QGDP+ WVSTHR HH++ D+  DPHSPI GFWF  + W FD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 144 SYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQRFYRFIHQTYWLHPLALS 203
           S  +  K C  Y                     +N  DL KQ +YRF+ +T+  H    +
Sbjct: 158 SKYIIEK-CGRY---------------------ENAGDLMKQSYYRFLERTFVYHVYLQA 217

Query: 204 FLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLSKNNWMVAIFTFG 263
            LLY  GG PFI+WGM VR +L  H + ++NSVCH WGN+PWNTGDLS NNW +A+ T G
Sbjct: 218 ALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSG 277

Query: 264 EGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLATNVKLPS--GKQKLAALN 318
           EGWHNNHHAFEYSARHG EWWQID  WYIIK L+ +GLAT++K+PS   K+K++  N
Sbjct: 278 EGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMSFKN 312

BLAST of PI0022374 vs. ExPASy TrEMBL
Match: A0A1S3BS49 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492608 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 4.8e-188
Identity = 312/329 (94.83%), Postives = 317/329 (96.35%), Query Frame = 0

Query: 1   MEEASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSL 60
           MEEASKEE KEM+MNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAP YFSWTCL LSL
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSL 60

Query: 61  GLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GLANLTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180
           SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QKVERN FMFYMKHGY DNVTD
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTD 180

Query: 181 LEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWG 240
           LEKQRFYRFIH+TYWLHPLALS LLYAIGGLPFIIWGMCVRFV+SLHFTSMINSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGL 300
           NQPWNTGDLSKNNW+VAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIG 
Sbjct: 241 NQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGF 300

Query: 301 ATNVKLPSGKQKLAALNKSNPTILKPMID 330
           ATNVKLPS KQK AALNKS PT LKPMID
Sbjct: 301 ATNVKLPSEKQKWAALNKSKPTFLKPMID 329

BLAST of PI0022374 vs. ExPASy TrEMBL
Match: A0A0A0KAB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.5e-181
Identity = 298/329 (90.58%), Postives = 311/329 (94.53%), Query Frame = 0

Query: 1   MEEASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSL 60
           M+E SKE+PKEM+M SP RLF+RKKWTKFD+NVAFYFL LHLLCILAP YFSWTCLLL+L
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 61  GLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GL+ LTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180
           S RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180

Query: 181 LEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWG 240
           LEKQ+FYRFIH+TYWLHPLALS LLYAIGGLPFIIWGMCVRFV SLH TSM+NSVCHIWG
Sbjct: 181 LEKQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGL 300
           NQPWNTGDLSKNNWMVA+ TFGEGWHNNHHAFEYSARHGHEWWQIDYGWY+I+FLQVIGL
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 301 ATNVKLPSGKQKLAALNKSNPTILKPMID 330
           ATNVKLPS KQKLAALNKS P  LK MID
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of PI0022374 vs. ExPASy TrEMBL
Match: A0A1S3BQG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492609 PE=3 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 7.6e-170
Identity = 282/328 (85.98%), Postives = 299/328 (91.16%), Query Frame = 0

Query: 3   EASKEEPKE-MMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLG 62
           E SKE+PKE ++M SP +LF+RKKWTKFD+N AFYFL  HLLCILAP  FSWT LLL+LG
Sbjct: 4   EVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLALG 63

Query: 63  LANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 122
           L+ L+GM ISVSYHRNLAHRSFKLPKW EYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS
Sbjct: 64  LSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 123

Query: 123 ERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDL 182
           ERDPHSPIYGFWFSQMTW FDSYNLTRKVCPNYFNNYQK ERNIFM YMKHGYPDNV DL
Sbjct: 124 ERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPDL 183

Query: 183 EKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGN 242
           EKQ FYRFIH+TYWLHPLALS LLYAIGGLPFIIWGMCVRFV S+H TSM+NSVCH+WGN
Sbjct: 184 EKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWGN 243

Query: 243 QPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLA 302
           QPWNTGDLSKNNWMVA+ TFGEGWHNNHHAFEYSARHG EWWQIDYGWY I+FL+VIGLA
Sbjct: 244 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGLA 303

Query: 303 TNVKLPSGKQKLAALNKSNPTILKPMID 330
           TNVK PS KQKLAALNKS PT LKP I+
Sbjct: 304 TNVKFPSEKQKLAALNKSKPTFLKPTIN 331

BLAST of PI0022374 vs. ExPASy TrEMBL
Match: A0A6J1JUL6 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488510 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.1e-163
Identity = 269/319 (84.33%), Postives = 291/319 (91.22%), Query Frame = 0

Query: 3   EASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGL 62
           EASK+ P   M+ SP  LF RKKWTKFD+NVA YFLLLHLLCILAP YF+WTC  L++ L
Sbjct: 2   EASKQHPN--MVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIIL 61

Query: 63  ANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122
           ANLTGMAISVSYHRNLAHR+FKLPKWLEYSLAY AAHAIQGDPIDWVSTHRCHHR+VD+E
Sbjct: 62  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 121

Query: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLE 182
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYF N++KVERN+FMF+MKHGYPDNV DLE
Sbjct: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 181

Query: 183 KQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQ 242
           KQ FYRF+ +TYWLHPLAL+ LLYA GG+P++IWGMCVRFVLSLH TSM+NSVCHIWGNQ
Sbjct: 182 KQWFYRFMRKTYWLHPLALAGLLYAAGGIPYVIWGMCVRFVLSLHVTSMVNSVCHIWGNQ 241

Query: 243 PWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 302
           PWNTGDLS+NNWMVA+ TFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT
Sbjct: 242 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 301

Query: 303 NVKLPSGKQKLAALNKSNP 322
            VKLPS    LAALNKS P
Sbjct: 302 CVKLPSQTHNLAALNKSKP 318

BLAST of PI0022374 vs. ExPASy TrEMBL
Match: A0A6J1GPG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456275 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 5.3e-163
Identity = 267/319 (83.70%), Postives = 292/319 (91.54%), Query Frame = 0

Query: 3   EASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGL 62
           EASK+ P   M+ SP  LF RKKWTKFD+NVA YFLLLHLLCILAP YF+WT   L++ L
Sbjct: 2   EASKQHPN--MVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTSFFLTIIL 61

Query: 63  ANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSE 122
           ANLTGMAISVSYHRNLAHR+FKLPKWLEYSLAY AAHAIQGDPIDWVSTHRCHHR+VD+E
Sbjct: 62  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 121

Query: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLE 182
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYF N++KVERN+FMF+MKHGYPDNV DLE
Sbjct: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 181

Query: 183 KQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQ 242
           KQ FYRF+ +TYWLHPLAL+ LLYA+GG+P++IWGMCVRFVLSLH TSM+NSVCHIWG Q
Sbjct: 182 KQWFYRFMRKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVLSLHITSMVNSVCHIWGTQ 241

Query: 243 PWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 302
           PWNTGDLS+NNWMVA+ TFGEGWHNNHHAFEYSARHGHEWW+IDYGWY+IKFLQVIGLAT
Sbjct: 242 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWEIDYGWYVIKFLQVIGLAT 301

Query: 303 NVKLPSGKQKLAALNKSNP 322
            VKLPS  QKLAALNKS P
Sbjct: 302 CVKLPSQTQKLAALNKSKP 318

BLAST of PI0022374 vs. NCBI nr
Match: XP_008451258.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 666.8 bits (1719), Expect = 9.8e-188
Identity = 312/329 (94.83%), Postives = 317/329 (96.35%), Query Frame = 0

Query: 1   MEEASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSL 60
           MEEASKEE KEM+MNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAP YFSWTCL LSL
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSL 60

Query: 61  GLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GLANLTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180
           SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QKVERN FMFYMKHGY DNVTD
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTD 180

Query: 181 LEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWG 240
           LEKQRFYRFIH+TYWLHPLALS LLYAIGGLPFIIWGMCVRFV+SLHFTSMINSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGL 300
           NQPWNTGDLSKNNW+VAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIG 
Sbjct: 241 NQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGF 300

Query: 301 ATNVKLPSGKQKLAALNKSNPTILKPMID 330
           ATNVKLPS KQK AALNKS PT LKPMID
Sbjct: 301 ATNVKLPSEKQKWAALNKSKPTFLKPMID 329

BLAST of PI0022374 vs. NCBI nr
Match: XP_011659770.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus])

HSP 1 Score: 659.8 bits (1701), Expect = 1.2e-185
Identity = 311/330 (94.24%), Postives = 321/330 (97.27%), Query Frame = 0

Query: 1   MEEASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPL-YFSWTCLLLS 60
           ME ASKE+PKEM+M+SPSRLFKRKKWTKFDKNVAFYFLLLHLL ILAPL YFSW CLLLS
Sbjct: 1   MEGASKEDPKEMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60

Query: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120
           LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV
Sbjct: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120

Query: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT 180
           DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT
Sbjct: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT 180

Query: 181 DLEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIW 240
           DLEKQ+FYRFIH+TYWLHPLALS LLYA+GGLPFIIWG+CVRFVLSLHFTSMINSVCHIW
Sbjct: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240

Query: 241 GNQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIG 300
           GNQPWNTGDLSKNNW+VAIFTFGEGWHNNHHAFEYSARHGHEWWQID GWYIIKFLQVIG
Sbjct: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300

Query: 301 LATNVKLPSGKQKLAALNKSNPTILKPMID 330
           LATNVKLPS KQK+AALNKS PT L+PMID
Sbjct: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 330

BLAST of PI0022374 vs. NCBI nr
Match: XP_038899717.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 646.7 bits (1667), Expect = 1.1e-181
Identity = 299/329 (90.88%), Postives = 312/329 (94.83%), Query Frame = 0

Query: 1   MEEASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSL 60
           MEEASKE PKEM+M SP RLF+RKKWTKFD+NVAFYFL+LHLLCILAP YFSWTCL L+L
Sbjct: 1   MEEASKEGPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLILHLLCILAPFYFSWTCLFLAL 60

Query: 61  GLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GL+ LTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHR+VD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRYVD 120

Query: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180
           SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QKVERN FMFYMKHG+PDNVTD
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGHPDNVTD 180

Query: 181 LEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWG 240
           LEKQRFYRFIH+TYWLHPLALS LLYAIGG+PFIIWGMCVRFV SLH TSM+NSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSVLLYAIGGVPFIIWGMCVRFVFSLHVTSMVNSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGL 300
            QPWNTGDLSKNNWMVAI TFGEGWHNNHHAFEYSARHGHEWWQID+GWYIIKFLQVIGL
Sbjct: 241 KQPWNTGDLSKNNWMVAILTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGL 300

Query: 301 ATNVKLPSGKQKLAALNKSNPTILKPMID 330
           ATNVKLPS KQKLAALNKS PT LKPMID
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPTFLKPMID 329

BLAST of PI0022374 vs. NCBI nr
Match: XP_011659261.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KAE8646293.1 hypothetical protein Csa_016268 [Cucumis sativus])

HSP 1 Score: 641.7 bits (1654), Expect = 3.4e-180
Identity = 297/329 (90.27%), Postives = 310/329 (94.22%), Query Frame = 0

Query: 1   MEEASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSL 60
           M+E SKE+PKEM+M SP RLF+RKKWTKFD+NVAFYFL LHLLCILAP YFSWTCLLL+L
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 61  GLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120
           GL+ LTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180
           S RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVTD
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180

Query: 181 LEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWG 240
           LEKQRFYRF+H+TYWLHPLALS LLYAIGGLPFIIWGMCVRFV SLH TSM+NSVCHIWG
Sbjct: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGL 300
           NQPWNTGDLSKNNWMVA+ TFGEGWHNNHHAFEYSARHGHEWWQIDYGWY+I+FLQVIGL
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 301 ATNVKLPSGKQKLAALNKSNPTILKPMID 330
           ATNVKLPS KQKLAALNKS P  LK MID
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of PI0022374 vs. NCBI nr
Match: XP_008451259.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 606.3 bits (1562), Expect = 1.6e-169
Identity = 282/328 (85.98%), Postives = 299/328 (91.16%), Query Frame = 0

Query: 3   EASKEEPKE-MMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLG 62
           E SKE+PKE ++M SP +LF+RKKWTKFD+N AFYFL  HLLCILAP  FSWT LLL+LG
Sbjct: 4   EVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLALG 63

Query: 63  LANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 122
           L+ L+GM ISVSYHRNLAHRSFKLPKW EYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS
Sbjct: 64  LSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDS 123

Query: 123 ERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDL 182
           ERDPHSPIYGFWFSQMTW FDSYNLTRKVCPNYFNNYQK ERNIFM YMKHGYPDNV DL
Sbjct: 124 ERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPDL 183

Query: 183 EKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGN 242
           EKQ FYRFIH+TYWLHPLALS LLYAIGGLPFIIWGMCVRFV S+H TSM+NSVCH+WGN
Sbjct: 184 EKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWGN 243

Query: 243 QPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLA 302
           QPWNTGDLSKNNWMVA+ TFGEGWHNNHHAFEYSARHG EWWQIDYGWY I+FL+VIGLA
Sbjct: 244 QPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGLA 303

Query: 303 TNVKLPSGKQKLAALNKSNPTILKPMID 330
           TNVK PS KQKLAALNKS PT LKP I+
Sbjct: 304 TNVKFPSEKQKLAALNKSKPTFLKPTIN 331

BLAST of PI0022374 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 355.9 bits (912), Expect = 3.5e-98
Identity = 165/290 (56.90%), Postives = 203/290 (70.00%), Query Frame = 0

Query: 24  KKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGLANLTG-MAISVSYHRNLAHRS 83
           ++W   D       L +HLL +LAP  F+W  + ++ GL  +TG + I++S+HRNL+H++
Sbjct: 96  REWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKA 155

Query: 84  FKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIYGFWFSQMTWFFD 143
           FKLPKWLEY  AY  A A+QG+PIDWVSTHR HH+F DS+RDPHSP+ GFWFS M W FD
Sbjct: 156 FKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFD 215

Query: 144 SYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQRFYRFIHQTYWLHPLALS 203
           +  +T++                       G P+NV DLEKQ FYRF+  TY LHPLAL+
Sbjct: 216 TNTITQRC----------------------GEPNNVGDLEKQPFYRFLRTTYILHPLALA 275

Query: 204 FLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLSKNNWMVAIFTFG 263
             LYA+GG PFI+WGM VR V   H T ++NS CH+WG Q WNTGDLSKNNW VA   FG
Sbjct: 276 VALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFG 335

Query: 264 EGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLATNVKLPSGKQK 313
           EGWHNNHHAFE+SARHG EWWQ+D  WY++KFLQ IGLAT+VKLPS  QK
Sbjct: 336 EGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQK 363

BLAST of PI0022374 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 322.8 bits (826), Expect = 3.2e-88
Identity = 151/301 (50.17%), Postives = 193/301 (64.12%), Query Frame = 0

Query: 11  EMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGLANLTGMAI 70
           E+++    R F  + WT +D +    F+  HLL +LAP YFSW    +   L  + G+ I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 71  SVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSERDPHSPIY 130
           ++SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  +++RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 131 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEKQRFYRFI 190
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRF+
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 191 HQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQPWNTGDLS 250
            +T  LH +A SFL Y  GG+P ++WG+ +   + LH T ++NSVCHIWG + WNT D S
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 251 KNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLATNVKLPSGK 310
           KNNW VAI T GEGWHNNHHAFE+SARHG EWWQ+D  W +I+FL+ IGLATNVKLP+  
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 311 Q 312
           Q
Sbjct: 376 Q 354

BLAST of PI0022374 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 320.9 bits (821), Expect = 1.2e-87
Identity = 156/318 (49.06%), Postives = 200/318 (62.89%), Query Frame = 0

Query: 1   MEEASKEEPKEMM------MNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWT 60
           +  + KEE  + M      M    R    +KW + D   AF  L +H LC+LAP  F+W 
Sbjct: 3   LSASEKEENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWP 62

Query: 61  CLLLSLGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRC 120
            L ++L +  + G+ I+VSYHRNLAHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR 
Sbjct: 63  ALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRY 122

Query: 121 HHRFVDSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGY 180
           HH+F DS+RDPHSP  GFWFS + W FD+  L  K                       G 
Sbjct: 123 HHQFTDSDRDPHSPNEGFWFSHLLWLFDTGYLVEKC----------------------GR 182

Query: 181 PDNVTDLEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINS 240
             NV DL++Q +Y+F+ +T   H L   FLLY  GGL F+ WGM +   +  H T +INS
Sbjct: 183 RTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINS 242

Query: 241 VCHIWGNQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKF 300
           +CH+WG++ W T D S+N W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++F
Sbjct: 243 LCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRF 298

Query: 301 LQVIGLATNVKLPSGKQK 313
           L++IGLAT+VKLPS  Q+
Sbjct: 303 LEIIGLATDVKLPSESQR 298

BLAST of PI0022374 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 315.1 bits (806), Expect = 6.8e-86
Identity = 154/309 (49.84%), Postives = 198/309 (64.08%), Query Frame = 0

Query: 4   ASKEEPKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLLSLGLA 63
           A+ EE K+       R ++R  + KF  +       +H L +LAP YF+W+ L ++    
Sbjct: 19  AAVEEKKKRRWVFWDRRWRRLDYVKFSAS-----FTVHSLALLAPFYFTWSALWVTFLFY 78

Query: 64  NLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVDSER 123
            + G+ I+VSYHRNLAHRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DSER
Sbjct: 79  TIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSER 138

Query: 124 DPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTDLEK 183
           DPHSP  GFWFS + W +DS  L  K                       G   NV DL++
Sbjct: 139 DPHSPKEGFWFSHLLWIYDSAYLVSKC----------------------GRRANVEDLKR 198

Query: 184 QRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHIWGNQP 243
           Q FYRF+ +T   H L L F L+ +GG+ F+ WGM V   L +H T +INS+CHIWG + 
Sbjct: 199 QWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRT 258

Query: 244 WNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLATN 303
           W T D S+N W +++F+FGE WHNNHHAFE SAR G EWWQID  WYI++F ++IGLAT+
Sbjct: 259 WKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATD 300

Query: 304 VKLPSGKQK 313
           VK+P+  Q+
Sbjct: 319 VKVPTEAQR 300

BLAST of PI0022374 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 303.5 bits (776), Expect = 2.0e-82
Identity = 153/314 (48.73%), Postives = 197/314 (62.74%), Query Frame = 0

Query: 1   MEEASKEE--PKEMMMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPLYFSWTCLLL 60
           M E +K++   ++  +    R +  +KWT+FD   A     +HLLC+LAP  + W     
Sbjct: 1   MSETTKDDGSSQKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRF 60

Query: 61  SLGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRF 120
            + LA LT + I+ SYHRNL HRSFKLPKWLEY  AY A  A+QGDP+DWVS HR HH+F
Sbjct: 61  GIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQF 120

Query: 121 VDSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNV 180
            DS+RDPHSPI GFWFS + W FD+ +  R+ C                     G  +NV
Sbjct: 121 TDSDRDPHSPIEGFWFSHVLWIFDT-DYIREKC---------------------GRRNNV 180

Query: 181 TDLEKQRFYRFIHQTYWLHPLALSFLLYAIGGLPFIIWGMCVRFVLSLHFTSMINSVCHI 240
            DL++Q FYRF+ +T  LH LA   L+Y  GGLP++ W +    V+  H T ++NS CHI
Sbjct: 181 MDLKQQWFYRFLKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHI 240

Query: 241 WGNQPWNTGDLSKNNWMVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVI 300
            G+Q W T D S+N W +A+ T GE WHNNHHAFE SARHG EW+Q+D  WY+I F Q +
Sbjct: 241 CGSQAWQTNDTSRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQAL 292

Query: 301 GLATNVKLPSGKQK 313
           GLATNVKLP+  QK
Sbjct: 301 GLATNVKLPTDAQK 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X04.9e-9756.90Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
Q9LVZ34.6e-8750.17Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
O657971.7e-8649.06Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
Q9SID29.5e-8549.84Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=... [more]
P0DOW24.7e-8450.51Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BS494.8e-18894.83palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A0A0KAB12.5e-18190.58FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G3763... [more]
A0A1S3BQG57.6e-17085.98palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1JUL61.1e-16384.33palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GPG55.3e-16383.70palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
XP_008451258.19.8e-18894.83PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
XP_011659770.11.2e-18594.24palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_038899717.11.1e-18190.88palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
XP_011659261.23.4e-18090.27palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_008451259.11.6e-16985.98PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
Match NameE-valueIdentityDescription
AT3G15850.13.5e-9856.90fatty acid desaturase 5 [more]
AT3G15870.13.2e-8850.17Fatty acid desaturase family protein [more]
AT1G06080.11.2e-8749.06delta 9 desaturase 1 [more]
AT2G31360.16.8e-8649.8416:0delta9 desaturase 2 [more]
AT1G06100.12.0e-8248.73Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 75..95
score: 37.09
coord: 112..141
score: 44.04
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 12..314
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 66..305
e-value: 1.74792E-67
score: 207.408
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 52..291
e-value: 2.1E-13
score: 50.7
NoneNo IPR availablePANTHERPTHR11351:SF87FATTY ACID DESATURASE, TYPE 1, CORE-RELATEDcoord: 12..314

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0022374.1PI0022374.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water