PI0021844 (gene) Melon (PI 482460) v1

Overview
NamePI0021844
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionmethyltransferase-like protein 13
Locationchr01: 25834928 .. 25838587 (+)
RNA-Seq ExpressionPI0021844
SyntenyPI0021844
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACAGGGACGTGTCGAGCTGCAACACCTACAACTATGGCGATGCTGTCTACTGGGACGCTCGTTACCTGCAGGAGGCTGGTTCTTTTGATTGGTACCAGCGTTATTCTTCTCTTCGTCCCTTTGTTCGTAAGTTCATCAGCACTTCCGCCTCCGTCCTTATGGTCGGCTGTGGTAATGCTGGTAATTTCCTCCTATTTCACCTTTCTTCCTTTTTCCAGTTTCTTTTTCTTTTTAGATTTCGATTGTTCTCGAATATGTGTTGGAAGTTGGAATTCGAATAAATTTTCGATGCATTCAATTTTTCGTCGTCCGAAATTAATGTTTGGCGGATTGAGAGATTTAGGCGGTTTAGCATGCGTATAAATTGAAACGAGGAGCTGTCTTGGAATCTTGTTCCTGAGAATTCGTAGTCAACTATTTGGACTCTGTTGGATGAGAATTACACGTTTGACGGAATGAACCGGAAAACCTGGTTTTGGTATTCAAATTTTTAATGTTAATCCTAACTTAAGTAGCTTAATATGTGGATTAGCAGAATGGAAGGCCAAGAAGTTAATGGATGAGAGGGGGTTCAAGTAACTGATTATCGAGGTTGTAAGCTGTATTTGGAAATTGGAATTTCTGAATTTGGTTGTTTCTGAAAATGTGTACATATTTGGCTAAAATATCCGCAATAACACGGGTTAAGATACAAGAATTAGTAAGTATTTGGAAGTTATAGACGCTGTATTGTTTGATTATTAATCTATATAATGTAAAAGATCCTGTATAAAAATTGATAACTGATGCAGTGTAATTTAGAGCTTCGGCACATTAAATATAACCTTATTGTTGTCTACTACTTACATTCACTTTAACCGTGACATCTTTTGTACTTGTAGTCATGTCAGAAGATATGGTCAAGGATGGGTATGAAGACATAATGAACGTTGACATTTCTTCAGTAGCAATCGACATGATGAAAAGAAAGTATCAGTTCATCCCCAGTTGAAATGTATCCTCTGAGGCTTGTATCTTTGTTTTGTTTTCAAATTAACACTCGAGTTGCTTATGTTGACAGAATAGTTAGTATGGTGATCTACATAGTACTTAGAGAGAGAGAGAGAGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAATTAATATGGTCATCTTAGCCATAATTATTAAGTTTGTTAAAATTGAATCATAAATACTTCAGTAAAGTGTAGGAGATATGGTTCTCTCAATAGGTTATCGTCCTTGTATTCCCTTTTAGCTAAAGTACAATCGGCTCGTACAAGTTCTACAAGTTTCCTTGACAAATCTGTATAGATATGGAAATGGATGTCAGGGACATGAGCTTTTTCCCAGACGAGAAATTTGGTGCTGTCATTGATAAAGGTCTGGCTTCTTGATCTTCTTTTACCCACCGTTGAATTTCCCAGTCTTTAATACTGAAGTTTGCTCTATGTGTTGATCTTTCAATGCTGGTGCCACCACGTTTACTCCTGGCCTCTTGATTCTTGATATAGGGACACTTGATTCATTGATGGTATGTGAGCTTTCCATATTTCTCATGCATGTTATTCACTATTTCCCCATGTTCTAATTCTTTAATTTTGTGTACACAGTGTGGCACTGATGCTCCGATTAGTGCTGCTCAAATGCTGGGAGAAGTGAGTAGGTTGTGGACTTTATGAACTTCACAAGATTGGTTGACTATTTTAGATTCTCTTCTTTGGTTTTATATTTCTCCACTAATTTTTGGCTGTTACTCCTGTAGGCTTCTAAACCAGGAGGAGTCTATTTACTGTATCCATCTTTTATCTCTCGTCAAGACTTTACTTTTCAATGTTCTGTTTGGAGTAAAATAATCAAAATAGATTTTTGGTGGTTTGCAGATAACATATGGTGATCCCAAAGTAAGGATGCCCATTTGATGCGACCTTCATACAATTGGAAGATAGCGTTTGTTTATAATCCGTGAGTTCGTTTTCCTCCTCTCACTGAACTTTTCTTTTTATTTCCAGGTCTCTAGTTGATACAGGTGTGTTTATTTGGTTTCTCCTGCTTTTGTTTCAAACATGTCTGCAGAAAAAACAATTTAAAGAACAAAAAAAATGAAGAATCGTAGTCAACTACTAAAAATAGTTAGTTTAATATTAGATTTCAAGAGCTCTCTCTGAGTGTTGAAAGCTCTGAACATGAACTATGGATGCAGGTAAGTTAAGCCATATTACTAAGATGTGCAACGGGGTGCGTGAGAGTATTAGTAAGGATATTTGGGAATATTAGTAGGATAACAATAAGGATTTAGTGGTAATTAGATGGTGCACTTGTTATAAATAAGGGGAGTTGGGCACCTTAGGAGGGAATCAAAAGACCATTGATTTTGTAATTTATTATCGATATTACATTATAGACATCTTTAGTGACATTTTCTGTGTTTTGGTATCCTAACAGTGGCCTTTTGGGCCATTTCTTTCTCTAGGCGTGTTTTCACCCATGAGAAAATATATGATTTATAGAGTGACAAGACAGAAGGACAAATGAACTTCTGAAGTGGTATTTATTGGAAGAAGTGCTATTACGATTCCCAGTTAGTGTTGTAGCCTAAGGAATTAAAGGAGAGCATGCTTCAAGAGTCAAATCAATATCAGTAAAAAATTCCTGAATACGTGATACCACAGGCAAGAAACTACGAATTAAAATACAAAAAAGAAACAAAAAGGAAAACTGAAATTTAACAGGAAACTAAAAGGATCATTCAAGGATTTAACCAAGAGTAATATGAATTCTACATCAGTTAAAACTAGCTTTCCAGTGTTTTGGTTTGCTCCCTACTATGCGGTCGGGGTTTTGGATGCATTGTGATGTTGGCCACGCTGCCCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTGCAAAAATGTACCTTATTTTTGGATTTTTTATTATTAGACCCATATATGTTTATTTGGGTTTTAATTCCTTGAGTTAGGGGTAGAATGTTCTTTTAGTGTAGTAGGATAAGTTTAAAGTAGGGAAACAAAATACACACATTAGTTTTTTATAATTTTAAAGATTTAGAGTTTTCTATCAATGAAAATTCTCTGGTAGATGACAAAATCTCATCACCACTCTTTTGCAATTCTTGACTCAGGTTTGTATGCTAAAAACATTTAGGAAAGTTTGATGATCACATTGCTTATGGAGTGTTTGAAATTGGAGTGAGGAACTAGTTCTCATTTATCTTTTGGTCTTCGATCAAAGTGGTAATTCAAATAAATCTATCACCTTGGGTTATTGTCAAATTGATTTGGAAGTCTTAGAATTTGATATTAGGTGTTCACCAAGTGTGGGTTCCTAATTTTTAAATTCAGGGTTTTCATATTAGTAGTTGAACTATAAGAAATGCTAAATTATAAAAACTATTCTCGATCAACTTTGCCCTAGTATAATAATATTAAATTACGTCAACGTCATTTGAAATGACCAATCAAGTCTTCCTAATTTTAGAGTCGAAAATATATATATATTAACCTCTCGAGTTTATAGTAATGTTGAAAGGATAAGATCAGGATGA

mRNA sequence

ATGTACAGGGACGTGTCGAGCTGCAACACCTACAACTATGGCGATGCTGTCTACTGGGACGCTCGTTACCTGCAGGAGGCTGGTTCTTTTGATTGGTACCAGCGTTATTCTTCTCTTCGTCCCTTTGTTCGTAAGTTCATCAGCACTTCCGCCTCCGTCCTTATGGTCGGCTGTGGTAATGCTGTCATGTCAGAAGATATGGTCAAGGATGGGTATGAAGACATAATGAACGTTGACATTTCTTCAGTAGCAATCGACATGATGAAAAGAAAGTATCAGTTCATCCCCAATATGGAAATGGATGTCAGGGACATGAGCTTTTTCCCAGACGAGAAATTTGGTGCTGTCATTGATAAAGGGACACTTGATTCATTGATGTGTGGCACTGATGCTCCGATTAGTGCTGCTCAAATGCTGGGAGAAGCTTCTAAACCAGGAGGAGTCTATTTACTTAAGGATGCCCATTTGATGCGACCTTCATACAATTGGAAGATAGCGTTTGTTTATAATCCTCTTCCTAATTTTAGAGTCGAAAATATATATATATTAACCTCTCGAGTTTATAGTAATGTTGAAAGGATAAGATCAGGATGA

Coding sequence (CDS)

ATGTACAGGGACGTGTCGAGCTGCAACACCTACAACTATGGCGATGCTGTCTACTGGGACGCTCGTTACCTGCAGGAGGCTGGTTCTTTTGATTGGTACCAGCGTTATTCTTCTCTTCGTCCCTTTGTTCGTAAGTTCATCAGCACTTCCGCCTCCGTCCTTATGGTCGGCTGTGGTAATGCTGTCATGTCAGAAGATATGGTCAAGGATGGGTATGAAGACATAATGAACGTTGACATTTCTTCAGTAGCAATCGACATGATGAAAAGAAAGTATCAGTTCATCCCCAATATGGAAATGGATGTCAGGGACATGAGCTTTTTCCCAGACGAGAAATTTGGTGCTGTCATTGATAAAGGGACACTTGATTCATTGATGTGTGGCACTGATGCTCCGATTAGTGCTGCTCAAATGCTGGGAGAAGCTTCTAAACCAGGAGGAGTCTATTTACTTAAGGATGCCCATTTGATGCGACCTTCATACAATTGGAAGATAGCGTTTGTTTATAATCCTCTTCCTAATTTTAGAGTCGAAAATATATATATATTAACCTCTCGAGTTTATAGTAATGTTGAAAGGATAAGATCAGGATGA

Protein sequence

MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGNAVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPNMEMDVRDMSFFPDEKFGAVIDKGTLDSLMCGTDAPISAAQMLGEASKPGGVYLLKDAHLMRPSYNWKIAFVYNPLPNFRVENIYILTSRVYSNVERIRSG
Homology
BLAST of PI0021844 vs. ExPASy Swiss-Prot
Match: Q91YR5 (eEF1A lysine and N-terminal methyltransferase OS=Mus musculus OX=10090 GN=EEF1AKNMT PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 6.0e-18
Identity = 58/160 (36.25%), Postives = 90/160 (56.25%), Query Frame = 0

Query: 13  YGDAVYWDARYLQEAG--SFDWYQRYSSLRPFVRKFISTSASVLMVGCGNAVMSEDMVKD 72
           +G A YW+ ++ Q+ G  +F+WY  Y  L   + K+I     VL++GCGN+ +SE +   
Sbjct: 11  FGSADYWE-KFFQQRGKTAFEWYGTYLELCEVLHKYIKPKEKVLVIGCGNSELSEQLYDV 70

Query: 73  GYEDIMNVDISSVAIDMMK-RKYQFIPNMEMDVRDMS--FFPDEKFGAVIDKGTLDSLMC 132
           GY+DI+N+DIS V I  MK R     P+M     DM+   FPD  F  V+DKGTLD+++ 
Sbjct: 71  GYQDIVNIDISEVVIKQMKERNGSRRPHMSFLKMDMTQLEFPDATFQVVLDKGTLDAVLT 130

Query: 133 GTDAPISAAQM------LGEASKPGGVYL---LKDAHLMR 159
             +  ++  Q+      +G   + GG YL   L  AH+++
Sbjct: 131 -DEEEVTLRQVDRMLAEVGRVLQVGGRYLCISLAQAHILK 168

BLAST of PI0021844 vs. ExPASy Swiss-Prot
Match: A5PK19 (eEF1A lysine and N-terminal methyltransferase OS=Bos taurus OX=9913 GN=EEF1AKNMT PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 7.8e-18
Identity = 61/162 (37.65%), Postives = 88/162 (54.32%), Query Frame = 0

Query: 13  YGDAVYWDARYLQEAG--SFDWYQRYSSLRPFVRKFISTSASVLMVGCGNAVMSEDMVKD 72
           +G   YW+ ++ Q+ G  +F+WY  Y  L   + K+I     VL+VGCGN+ +SE +   
Sbjct: 11  FGSVDYWE-KFFQQRGKKAFEWYGTYLELCGVLHKYIKPREKVLVVGCGNSELSEQLYDV 70

Query: 73  GYEDIMNVDISSVAIDMMK-RKYQFIPNM---EMDVRDMSFFPDEKFGAVIDKGTLDSLM 132
           GY+DI+N+DIS V I  MK R     P M   +MD+  M  FPD  F  V+DKGTLD+++
Sbjct: 71  GYQDIVNIDISEVVIKQMKERNASRRPRMSFLKMDMTQME-FPDASFQVVLDKGTLDAVL 130

Query: 133 CGTDAPISAAQM-------LGEASKPGGVYL---LKDAHLMR 159
             TD      Q        +G   + GG YL   L  AH+++
Sbjct: 131 --TDEEEKTLQQVDRMLAEVGRVLQVGGRYLCISLAQAHVLK 168

BLAST of PI0021844 vs. ExPASy Swiss-Prot
Match: P0DPD7 (EEF1A lysine methyltransferase 4 OS=Homo sapiens OX=9606 GN=EEF1AKMT4 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.0e-17
Identity = 53/177 (29.94%), Postives = 90/177 (50.85%), Query Frame = 0

Query: 13  YGDAVYWDARYLQEAGS--FDWYQRYSSLRPFVRKFISTSASVLMVGCGNAVMSEDMVKD 72
           Y +  YWD RY   A S  +DW+  +SS R  +   +     +L++GCGN+ +S ++   
Sbjct: 20  YREVEYWDQRYQGAADSAPYDWFGDFSSFRALLEPELRPEDRILVLGCGNSALSYELFLG 79

Query: 73  GYEDIMNVDISSVAIDMMKRKYQFIPNME---MDVRDMSFFPDEKFGAVIDKGTLDSLMC 132
           G+ ++ +VD SSV +  M+ ++  +P +    MDVR +  FP   F  V++KGTLD+L+ 
Sbjct: 80  GFPNVTSVDYSSVVVAAMQARHAHVPQLRWETMDVRKLD-FPSASFDVVLEKGTLDALLA 139

Query: 133 GTDAPISAA--------QMLGEASK---PGGVYL--------LKDAHLMRPSYNWKI 166
           G   P + +        Q+L E S+   PGG ++         +  H  +  Y W +
Sbjct: 140 GERDPWTVSSEGVHTVDQVLSEVSRVLVPGGRFISMTSAAPHFRTRHYAQAYYGWSL 195

BLAST of PI0021844 vs. ExPASy Swiss-Prot
Match: Q8N6R0 (eEF1A lysine and N-terminal methyltransferase OS=Homo sapiens OX=9606 GN=EEF1AKNMT PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.0e-17
Identity = 57/162 (35.19%), Postives = 87/162 (53.70%), Query Frame = 0

Query: 13  YGDAVYWDARYLQEAG--SFDWYQRYSSLRPFVRKFISTSASVLMVGCGNAVMSEDMVKD 72
           +G   YW+ ++ Q+ G  +F+WY  Y  L   + K+I     VL++GCGN+ +SE +   
Sbjct: 11  FGSVDYWE-KFFQQRGKKAFEWYGTYLELCGVLHKYIKPREKVLVIGCGNSELSEQLYDV 70

Query: 73  GYEDIMNVDISSVAIDMMKR----KYQFIPNMEMDVRDMSFFPDEKFGAVIDKGTLDSLM 132
           GY DI+N+DIS V I  MK     +   +  ++MD+  M  FPD  F  V+DKGTLD+++
Sbjct: 71  GYRDIVNIDISEVVIKQMKECNATRRPQMSFLKMDMTQME-FPDASFQVVLDKGTLDAVL 130

Query: 133 CGTDAPISAAQM-------LGEASKPGGVYL---LKDAHLMR 159
             TD      Q        +G   + GG YL   L  AH+++
Sbjct: 131 --TDEEEKTLQQVDRMLAEVGRVLQVGGRYLCISLAQAHILK 168

BLAST of PI0021844 vs. ExPASy Swiss-Prot
Match: P0DPE1 (EEF1A lysine methyltransferase 4 OS=Bos taurus OX=9913 GN=EEF1AKMT4 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.9e-16
Identity = 52/177 (29.38%), Postives = 88/177 (49.72%), Query Frame = 0

Query: 13  YGDAVYWDARYLQEAGS--FDWYQRYSSLRPFVRKFISTSASVLMVGCGNAVMSEDMVKD 72
           Y +  YWD RY   A S  ++W+  +S  R  +   +     +L++GCGN+ +S ++   
Sbjct: 20  YREVQYWDQRYQGAADSAPYEWFGDFSCFRDLLEPELRPLDRILVLGCGNSALSYEIFLG 79

Query: 73  GYEDIMNVDISSVAIDMMKRKYQFIPNME---MDVRDMSFFPDEKFGAVIDKGTLDSLMC 132
           G+ D+ +VD SSV +  M+ +Y  +P +    MDVR +  FP   F  V++KGTLD+L+ 
Sbjct: 80  GFPDVTSVDYSSVVVAAMRARYAHVPTLRWETMDVRALG-FPSGSFDVVLEKGTLDALLT 139

Query: 133 GTDAPISAA--------QMLGEASK---PGGVYL--------LKDAHLMRPSYNWKI 166
           G   P + +        Q+L E S+   P G ++         +  H  +  Y W +
Sbjct: 140 GEQDPWTVSSEGVHTVDQVLNEVSRVLVPAGRFISLTSAAPHFRTRHYAQAHYGWSL 195

BLAST of PI0021844 vs. ExPASy TrEMBL
Match: A0A0A0K6H6 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G420810 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.5e-83
Identity = 163/190 (85.79%), Postives = 166/190 (87.37%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIP    MEMDVRDMSFFPDEKFGAVI
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS---KPGGVYLL--------KDAHLMRPSYNWKIA 177
           DKGTLDSLMCGTDAPISAAQMLGE S   KPGGVYLL        +  HLMRPSYNWKIA
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIA 180

BLAST of PI0021844 vs. ExPASy TrEMBL
Match: A0A5D3CGK5 (Methyltransferase-like protein 13 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00240 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 9.4e-83
Identity = 162/190 (85.26%), Postives = 165/190 (86.84%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTYNYGDAVYWDARYL EAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLHEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIP    MEMDVRDMSFFPDEKFGAVI
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS---KPGGVYLL--------KDAHLMRPSYNWKIA 177
           DKGTLDSLMCGTDAPISAAQMLGE S   KPGGVYLL        +  HLMRPSYNWKIA
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIA 180

BLAST of PI0021844 vs. ExPASy TrEMBL
Match: A0A1S3CE67 (methyltransferase-like protein 13 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499963 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 9.4e-83
Identity = 162/190 (85.26%), Postives = 165/190 (86.84%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTYNYGDAVYWDARYL EAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLHEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIP    MEMDVRDMSFFPDEKFGAVI
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS---KPGGVYLL--------KDAHLMRPSYNWKIA 177
           DKGTLDSLMCGTDAPISAAQMLGE S   KPGGVYLL        +  HLMRPSYNWKIA
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIA 180

BLAST of PI0021844 vs. ExPASy TrEMBL
Match: A0A5A7URY3 (Methyltransferase-like protein 13 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00490 PE=4 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.2e-82
Identity = 162/191 (84.82%), Postives = 165/191 (86.39%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTYNYGDAVYWDARYL EAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLHEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIP    MEMDVRDMSFFPDEKFGAVI
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS----KPGGVYLL--------KDAHLMRPSYNWKI 177
           DKGTLDSLMCGTDAPISAAQMLGE S    KPGGVYLL        +  HLMRPSYNWKI
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKI 180

BLAST of PI0021844 vs. ExPASy TrEMBL
Match: A0A6J1KQY8 (methyltransferase-like protein 13 OS=Cucurbita maxima OX=3661 GN=LOC111497867 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 5.7e-80
Identity = 153/189 (80.95%), Postives = 164/189 (86.77%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTY+YGDAVYWDARYLQEAGSFDWYQRY++LRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYDYGDAVYWDARYLQEAGSFDWYQRYAALRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDM+KDGYEDIMN+DISSVAID+M+RKYQFIP    M+MDV+DMSFFPDEKFGAV+
Sbjct: 61  AVMSEDMIKDGYEDIMNIDISSVAIDIMRRKYQFIPQLKYMQMDVKDMSFFPDEKFGAVV 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS---KPGGVYLL--------KDAHLMRPSYNWKIA 176
           DKGTLDSLMCGTDAPISAAQMLGE S   KPGGVYLL        +  HLMRPSYNWKI 
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIT 180

BLAST of PI0021844 vs. NCBI nr
Match: XP_004136132.1 (EEF1A lysine methyltransferase 4 isoform X3 [Cucumis sativus] >KGN45088.1 hypothetical protein Csa_016639 [Cucumis sativus])

HSP 1 Score: 318.2 bits (814), Expect = 5.1e-83
Identity = 163/190 (85.79%), Postives = 166/190 (87.37%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIP    MEMDVRDMSFFPDEKFGAVI
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS---KPGGVYLL--------KDAHLMRPSYNWKIA 177
           DKGTLDSLMCGTDAPISAAQMLGE S   KPGGVYLL        +  HLMRPSYNWKIA
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIA 180

BLAST of PI0021844 vs. NCBI nr
Match: XP_008461358.1 (PREDICTED: methyltransferase-like protein 13 isoform X1 [Cucumis melo] >TYK10382.1 methyltransferase-like protein 13 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 316.2 bits (809), Expect = 1.9e-82
Identity = 162/190 (85.26%), Postives = 165/190 (86.84%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTYNYGDAVYWDARYL EAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLHEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIP    MEMDVRDMSFFPDEKFGAVI
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS---KPGGVYLL--------KDAHLMRPSYNWKIA 177
           DKGTLDSLMCGTDAPISAAQMLGE S   KPGGVYLL        +  HLMRPSYNWKIA
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIA 180

BLAST of PI0021844 vs. NCBI nr
Match: KAA0058582.1 (methyltransferase-like protein 13 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 315.8 bits (808), Expect = 2.5e-82
Identity = 162/191 (84.82%), Postives = 165/191 (86.39%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTYNYGDAVYWDARYL EAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLHEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIP    MEMDVRDMSFFPDEKFGAVI
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS----KPGGVYLL--------KDAHLMRPSYNWKI 177
           DKGTLDSLMCGTDAPISAAQMLGE S    KPGGVYLL        +  HLMRPSYNWKI
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKI 180

BLAST of PI0021844 vs. NCBI nr
Match: XP_031744225.1 (EEF1A lysine methyltransferase 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 315.8 bits (808), Expect = 2.5e-82
Identity = 163/196 (83.16%), Postives = 166/196 (84.69%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIP    MEMDVRDMSFFPDEKFGAVI
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS---KPGGVYLL--------------KDAHLMRPS 177
           DKGTLDSLMCGTDAPISAAQMLGE S   KPGGVYLL              +  HLMRPS
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLIFRGLQITYGDPKVRMPHLMRPS 180

BLAST of PI0021844 vs. NCBI nr
Match: XP_031744227.1 (EEF1A lysine methyltransferase 4 isoform X4 [Cucumis sativus])

HSP 1 Score: 314.3 bits (804), Expect = 7.4e-82
Identity = 161/180 (89.44%), Postives = 162/180 (90.00%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN
Sbjct: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIP    MEMDVRDMSFFPDEKFGAVI
Sbjct: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPQLKYMEMDVRDMSFFPDEKFGAVI 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS---KPGGVYLL--------KDAHLMRPSYNWKIA 167
           DKGTLDSLMCGTDAPISAAQMLGE S   KPGGVYLL        +  HLMRPSYNWKIA
Sbjct: 121 DKGTLDSLMCGTDAPISAAQMLGEVSRLLKPGGVYLLITYGDPKVRMPHLMRPSYNWKIA 180

BLAST of PI0021844 vs. TAIR 10
Match: AT3G60910.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 254.6 bits (649), Expect = 6.5e-68
Identity = 127/190 (66.84%), Postives = 149/190 (78.42%), Query Frame = 0

Query: 1   MYRDVSSCNTYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGN 60
           M RDVSSCNTYNYGDA+YWDARY+Q+A SFDWYQ YSSLRPFVR F+STS+ VLMVGCGN
Sbjct: 1   MQRDVSSCNTYNYGDALYWDARYVQDALSFDWYQCYSSLRPFVRSFVSTSSRVLMVGCGN 60

Query: 61  AVMSEDMVKDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVI 120
           ++MSEDMVKDGYEDIMNVDISSVAI+MM+ KY  +P    M+MDVRDMS+F D+ F  +I
Sbjct: 61  SLMSEDMVKDGYEDIMNVDISSVAIEMMQTKYASVPQLKYMQMDVRDMSYFEDDSFDTII 120

Query: 121 DKGTLDSLMCGTDAPISAAQMLGEAS---KPGGVYLL--------KDAHLMRPSYNWKIA 177
           DKGTLDSLMCG+DA +SA++MLGE S   KPGG Y L        +  HL R +YNWKI+
Sbjct: 121 DKGTLDSLMCGSDALLSASRMLGEVSRLIKPGGTYFLITYGDPKVRMPHLTRSAYNWKIS 180

BLAST of PI0021844 vs. TAIR 10
Match: AT3G17365.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 141.7 bits (356), Expect = 6.1e-34
Identity = 73/149 (48.99%), Postives = 98/149 (65.77%), Query Frame = 0

Query: 10  TYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFI-STSASVLMVGCGNAVMSEDMV 69
           T +Y +  YWD RY  E+  FDWYQ+YS L P +  ++   +  VL++GCGN+  SE MV
Sbjct: 7   TQSYSEQWYWDDRYKNESEPFDWYQKYSPLAPLINLYVPQRNQRVLVIGCGNSAFSEGMV 66

Query: 70  KDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVIDKGTLDSL 129
            DGYED++++DISSV ID M +KY   P    ++MDVRDM  F D  F AVIDKGTLDS+
Sbjct: 67  DDGYEDVVSIDISSVVIDTMIKKYSDRPQLKYLKMDVRDMKAFEDASFDAVIDKGTLDSI 126

Query: 130 MCGTDAPISAAQMLGE---ASKPGGVYLL 152
           +CG+++   + QML E     K  GVY+L
Sbjct: 127 LCGSNSRQYSTQMLEEVWRVLKDKGVYIL 155

BLAST of PI0021844 vs. TAIR 10
Match: AT3G17365.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-27
Identity = 59/121 (48.76%), Postives = 79/121 (65.29%), Query Frame = 0

Query: 10  TYNYGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFI-STSASVLMVGCGNAVMSEDMV 69
           T +Y +  YWD RY  E+  FDWYQ+YS L P +  ++   +  VL++GCGN+  SE MV
Sbjct: 7   TQSYSEQWYWDDRYKNESEPFDWYQKYSPLAPLINLYVPQRNQRVLVIGCGNSAFSEGMV 66

Query: 70  KDGYEDIMNVDISSVAIDMMKRKYQFIPN---MEMDVRDMSFFPDEKFGAVIDKGTLDSL 127
            DGYED++++DISSV ID M +KY   P    ++MDVRDM  F D  F AVIDKG L + 
Sbjct: 67  DDGYEDVVSIDISSVVIDTMIKKYSDRPQLKYLKMDVRDMKAFEDASFDAVIDKGVLTTC 126

BLAST of PI0021844 vs. TAIR 10
Match: AT4G34360.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 3.4e-16
Identity = 52/168 (30.95%), Postives = 85/168 (50.60%), Query Frame = 0

Query: 13  YGDAVYWDARYLQEAGSFDWYQRYSSLRPFVRKFISTSASVLMVGCGNAVMSEDMVKDGY 72
           Y D  YWD R+  E   ++W++ YS  +  +   I  S+SVL +GCGN+ + E++ KDG 
Sbjct: 15  YLDPHYWDERFSSEE-HYEWFKDYSHFQHLIISNIKPSSSVLELGCGNSQLCEELYKDGI 74

Query: 73  EDIMNVDISSVAIDMMKRK-----YQFIPNMEMDVRDMSFFPDEKFGAVIDKGTLDSLMC 132
            DI  +D+SSVA++ M+ +     Y+ I  ++ D+ D+  F  E F  VI+KGT+D L  
Sbjct: 75  VDITCIDLSSVAVEKMQSRLLPKGYKEIKVVQADMLDLP-FDSESFDVVIEKGTMDVLFV 134

Query: 133 GTDAP-----------ISAAQMLGEASKPGGVYL---LKDAHLMRPSY 162
               P           ++    +    KP G+++       H  RP +
Sbjct: 135 DAGDPWNPRPETVSKVMATLDGVHRVLKPDGIFISITFGQPHFRRPLF 180

BLAST of PI0021844 vs. TAIR 10
Match: AT2G31740.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.4e-14
Identity = 55/158 (34.81%), Postives = 84/158 (53.16%), Query Frame = 0

Query: 29  SFDWY----QRYSSLRPFVRKFISTSA---SVLMVGCGNAVMSEDMVKDGYEDIMNVDIS 88
           SF+WY    Q + SL P ++   S+S+    +L+ GCGN+ ++E +   G+ DI NVD S
Sbjct: 41  SFEWYAEWPQLHDSLLPLLQDSSSSSSDSLQILVPGCGNSRLTEHLYDAGFRDITNVDFS 100

Query: 89  SVAI-DMMKRKYQFIPNMEMDVRDMS--FFPDEKFGAVIDKGTLDSLMCGTDAPISAAQM 148
            V I DM++R  +  P +   V D++     DE F  V+DKG LD+LM          Q 
Sbjct: 101 KVVISDMLRRNIRTRPELRWRVMDITKMQLADESFDTVLDKGALDALMEPEVGTKLGNQY 160

Query: 149 LGEAS---KPGGVYL---LKDAHLM-----RPSYNWKI 166
           L EA    KPGG ++   L ++H++     R  + WK+
Sbjct: 161 LSEAKRVLKPGGKFICLTLAESHVLALLFSRFRFGWKM 198

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q91YR56.0e-1836.25eEF1A lysine and N-terminal methyltransferase OS=Mus musculus OX=10090 GN=EEF1AK... [more]
A5PK197.8e-1837.65eEF1A lysine and N-terminal methyltransferase OS=Bos taurus OX=9913 GN=EEF1AKNMT... [more]
P0DPD73.0e-1729.94EEF1A lysine methyltransferase 4 OS=Homo sapiens OX=9606 GN=EEF1AKMT4 PE=1 SV=1[more]
Q8N6R03.0e-1735.19eEF1A lysine and N-terminal methyltransferase OS=Homo sapiens OX=9606 GN=EEF1AKN... [more]
P0DPE11.9e-1629.38EEF1A lysine methyltransferase 4 OS=Bos taurus OX=9913 GN=EEF1AKMT4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K6H62.5e-8385.79Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G42... [more]
A0A5D3CGK59.4e-8385.26Methyltransferase-like protein 13 isoform X1 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CE679.4e-8385.26methyltransferase-like protein 13 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5A7URY31.2e-8284.82Methyltransferase-like protein 13 isoform X1 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1KQY85.7e-8080.95methyltransferase-like protein 13 OS=Cucurbita maxima OX=3661 GN=LOC111497867 PE... [more]
Match NameE-valueIdentityDescription
XP_004136132.15.1e-8385.79EEF1A lysine methyltransferase 4 isoform X3 [Cucumis sativus] >KGN45088.1 hypoth... [more]
XP_008461358.11.9e-8285.26PREDICTED: methyltransferase-like protein 13 isoform X1 [Cucumis melo] >TYK10382... [more]
KAA0058582.12.5e-8284.82methyltransferase-like protein 13 isoform X1 [Cucumis melo var. makuwa][more]
XP_031744225.12.5e-8283.16EEF1A lysine methyltransferase 4 isoform X1 [Cucumis sativus][more]
XP_031744227.17.4e-8289.44EEF1A lysine methyltransferase 4 isoform X4 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G60910.16.5e-6866.84S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G17365.16.1e-3448.99S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G17365.21.9e-2748.76S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G34360.13.4e-1630.95S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT2G31740.12.4e-1434.81S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 10..189
e-value: 2.9E-43
score: 149.5
NoneNo IPR availablePANTHERPTHR12176:SF66S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEINcoord: 9..169
NoneNo IPR availablePANTHERPTHR12176SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEINcoord: 9..169
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 53..156
e-value: 3.5804E-5
score: 39.3355
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 54..149
e-value: 4.4E-9
score: 36.9
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 11..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0021844.1PI0021844.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity