PI0021497 (gene) Melon (PI 482460) v1

BLAST of PI0021497 vs. ExPASy Swiss-Prot
Match: Q07404 (Urease accessory protein UreH OS=Bacillus sp. (strain TB-90) OX=36824 GN=ureH PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.5e-09
Identity = 55/196 (28.06%), Postives = 85/196 (43.37%), Query Frame = 0

Query: 147 QSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIGCNRM-ESAAVGALWGCGHDAGQVIF 206
           Q +LL+    GF  G  H +  PDH+ A++ +     ++  S+  G  WG GH +  +IF
Sbjct: 3   QVDLLSILTLGFVLGIKHAME-PDHVIAVSTIVCQSKKLWRSSLAGVFWGIGHTSTLLIF 62

Query: 207 GLLFLLLKDKLHIEILRTWG---TIIVGVTLFVIGIMGIREASEIRTPFVVALDNGECDV 266
           G+  +L+K K+  E    W      +VG+ L   GI  I    +        L +   D 
Sbjct: 63  GMTIILMKKKISQE----WSMSLEFLVGIILVYFGISAILSLKKTHEHSHSRL-HLHTDH 122

Query: 267 GIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLLMF 326
            IY     P V            +   G++HGL   A M++L    +     G  ++L F
Sbjct: 123 PIYTYKGIPYV-----------KSLFIGIIHGLAGSAAMVLLTMSTVEKAWEGLLYILFF 181

Query: 327 LVGTVISMGSYTAFIG 339
             GTV+ M S+T  IG
Sbjct: 183 GAGTVLGMLSFTTLIG 181

BLAST of PI0021497 vs. ExPASy TrEMBL
Match: A0A1S3BJI9 (uncharacterized protein LOC103490284 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490284 PE=4 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.1e-181
Identity = 335/361 (92.80%), Postives = 340/361 (94.18%), Query Frame = 0

Query: 1   MER-LLYSSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPS 60
           MER LLYSSSPTSLNINPNSSL+LPRF RII S++PNFPL  RHG HPLGF S KNDKPS
Sbjct: 1   MERLLLYSSSPTSLNINPNSSLVLPRFARIIHSLRPNFPLPTRHGFHPLGFLSFKNDKPS 60

Query: 61  ISSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVF 120
            SSIPC  S N   DSIF SDSP  SPKPDFLK FA KEKVKSSLFVLSALALILIQPVF
Sbjct: 61  FSSIPCFSSPNSSADSIFQSDSPPDSPKPDFLKPFARKEKVKSSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQN AKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM 240
           IGC+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM
Sbjct: 181 IGCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM 240

Query: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300
           GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA
Sbjct: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVA 360
           LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIG+FSEALKDRVPRITERLTWVA
Sbjct: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGTFSEALKDRVPRITERLTWVA 360

BLAST of PI0021497 vs. ExPASy TrEMBL
Match: A0A0A0K0L7 (DsbD_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G012930 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.4e-172
Identity = 326/361 (90.30%), Postives = 332/361 (91.97%), Query Frame = 0

Query: 1   MERLLY-SSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPS 60
           MERLL+ SSSP+SLNINPNSSL+LPRF RI DS+KPNFPL  RHGL PL   SSK   PS
Sbjct: 1   MERLLFSSSSPSSLNINPNSSLLLPRFPRIFDSLKPNFPLPTRHGLRPLPEISSKYHNPS 60

Query: 61  ISSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVF 120
            S      S N  T SIFHSDSP ASPKP  LK FA K+KVKSSLFVLSALALILIQPVF
Sbjct: 61  FS------SPNSSTHSIFHSDSPPASPKPHSLKPFAPKDKVKSSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQN AKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM 240
           IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLF+IGIM
Sbjct: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFIIGIM 240

Query: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300
           GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA
Sbjct: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVA 360
           LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVA
Sbjct: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVA 355

BLAST of PI0021497 vs. ExPASy TrEMBL
Match: A0A6J1JJH7 (uncharacterized protein LOC111486376 OS=Cucurbita maxima OX=3661 GN=LOC111486376 PE=4 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 9.6e-166
Identity = 308/362 (85.08%), Postives = 328/362 (90.61%), Query Frame = 0

Query: 1   MERLLYSSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPSI 60
           M+RLLYS SPTS  INPN  L+LPRF R IDSVK NFPL  RHGL PLG  SSK +KPS+
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRR-IDSVKLNFPLPTRHGLRPLGLLSSKVEKPSM 60

Query: 61  SSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVFA 120
             I CL SSN  +DSI  SDS  +SPKPD L+HFAGK+KVK SLFVLSALALILIQPVFA
Sbjct: 61  PLIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFA 120

Query: 121 PAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSI 180
           PAAFASFQN AKTGGPAA AVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 PAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIMG 240
           GC+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTI+VGVTLFVIG++G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLG 240

Query: 241 IREASEIRTPFVVALDNGECDVGIYETLEKPMVVG--NKKSKKLGFATFATGVVHGLQPD 300
           IREASE+RTP VV L+NGECDVGIY+TLEKPMV+G  NKK KK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 301 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWV 360
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW 
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of PI0021497 vs. ExPASy TrEMBL
Match: A0A6J1EK24 (uncharacterized protein LOC111435202 OS=Cucurbita moschata OX=3662 GN=LOC111435202 PE=4 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.1e-164
Identity = 307/362 (84.81%), Postives = 325/362 (89.78%), Query Frame = 0

Query: 1   MERLLYSSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPSI 60
           M+RLLYS SPTS  INPN  L+LPRF R IDS K NFPL  RHGL PLG  SSK DKPS+
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRR-IDSAKLNFPLPTRHGLRPLGLFSSKVDKPSM 60

Query: 61  SSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVFA 120
             I CL SSN  +DSI  S+S   SPKPD L+HFAGK+KVK SLFVLSALALILIQPVFA
Sbjct: 61  PLIHCLSSSNSLSDSISTSNSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFA 120

Query: 121 PAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSI 180
           PAAFASFQN AKTGGPAA AVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 PAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIMG 240
           GC+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTI+VGVTLFVIGI+G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGILG 240

Query: 241 IREASEIRTPFVVALDNGECDVGIYETLEKPMVVG--NKKSKKLGFATFATGVVHGLQPD 300
           IREASE+RTP VV L+NGECDVGIY+TLE PMV+G  NKK KK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 301 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWV 360
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW 
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of PI0021497 vs. ExPASy TrEMBL
Match: A0A6J1I158 (uncharacterized protein LOC111468477 OS=Cucurbita maxima OX=3661 GN=LOC111468477 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 8.4e-162
Identity = 307/361 (85.04%), Postives = 321/361 (88.92%), Query Frame = 0

Query: 1   MERLLYSSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPSI 60
           MERLLYS SPTSL IN NSSL L   GR IDS+K NFPL   HGL PLG T  KN+KPS+
Sbjct: 1   MERLLYSPSPTSLKINLNSSLTLLWSGR-IDSLKLNFPLPTLHGLKPLGLTFCKNEKPSM 60

Query: 61  SSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVFA 120
           SS  CL SSN  +DSIF SDS   SPKPDFL+HFAGKEKVK SL VLSAL LILIQP  A
Sbjct: 61  SSFCCLSSSNWLSDSIFPSDSLAGSPKPDFLEHFAGKEKVKRSLCVLSALVLILIQPGLA 120

Query: 121 PAAFASFQN-GAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           PAAFASFQN  AKTGGPAA  VGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 PAAFASFQNAAAKTGGPAAAVVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM 240
           IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTI+VG+TLF+IG+M
Sbjct: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGMTLFIIGVM 240

Query: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300
           GIRE SE+RTP  VAL+NGECDVGIYETLEKPMVVG KK KK+GFATFATGVVHGLQPDA
Sbjct: 241 GIREVSEVRTP-CVALENGECDVGIYETLEKPMVVGKKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVA 360
           LMIVLPALALPSRVAGAAFLLMFLVGTV+SMGSYTAFIGSFSEALKDRVPRITERLTW A
Sbjct: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVLSMGSYTAFIGSFSEALKDRVPRITERLTWAA 359

BLAST of PI0021497 vs. NCBI nr
Match: XP_008447954.1 (PREDICTED: uncharacterized protein LOC103490284 isoform X1 [Cucumis melo])

HSP 1 Score: 644.8 bits (1662), Expect = 4.4e-181
Identity = 335/361 (92.80%), Postives = 340/361 (94.18%), Query Frame = 0

Query: 1   MER-LLYSSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPS 60
           MER LLYSSSPTSLNINPNSSL+LPRF RII S++PNFPL  RHG HPLGF S KNDKPS
Sbjct: 1   MERLLLYSSSPTSLNINPNSSLVLPRFARIIHSLRPNFPLPTRHGFHPLGFLSFKNDKPS 60

Query: 61  ISSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVF 120
            SSIPC  S N   DSIF SDSP  SPKPDFLK FA KEKVKSSLFVLSALALILIQPVF
Sbjct: 61  FSSIPCFSSPNSSADSIFQSDSPPDSPKPDFLKPFARKEKVKSSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQN AKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM 240
           IGC+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM
Sbjct: 181 IGCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM 240

Query: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300
           GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA
Sbjct: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVA 360
           LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIG+FSEALKDRVPRITERLTWVA
Sbjct: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGTFSEALKDRVPRITERLTWVA 360

BLAST of PI0021497 vs. NCBI nr
Match: XP_004144849.1 (uncharacterized protein LOC101208891 [Cucumis sativus] >KGN43255.1 hypothetical protein Csa_020471 [Cucumis sativus])

HSP 1 Score: 614.8 bits (1584), Expect = 4.9e-172
Identity = 326/361 (90.30%), Postives = 332/361 (91.97%), Query Frame = 0

Query: 1   MERLLY-SSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPS 60
           MERLL+ SSSP+SLNINPNSSL+LPRF RI DS+KPNFPL  RHGL PL   SSK   PS
Sbjct: 1   MERLLFSSSSPSSLNINPNSSLLLPRFPRIFDSLKPNFPLPTRHGLRPLPEISSKYHNPS 60

Query: 61  ISSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVF 120
            S      S N  T SIFHSDSP ASPKP  LK FA K+KVKSSLFVLSALALILIQPVF
Sbjct: 61  FS------SPNSSTHSIFHSDSPPASPKPHSLKPFAPKDKVKSSLFVLSALALILIQPVF 120

Query: 121 APAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180
           APAAFASFQN AKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS
Sbjct: 121 APAAFASFQNAAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLS 180

Query: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIM 240
           IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLF+IGIM
Sbjct: 181 IGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFIIGIM 240

Query: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300
           GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA
Sbjct: 241 GIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVA 360
           LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVA
Sbjct: 301 LMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVA 355

BLAST of PI0021497 vs. NCBI nr
Match: XP_022989236.1 (uncharacterized protein LOC111486376 [Cucurbita maxima])

HSP 1 Score: 592.8 bits (1527), Expect = 2.0e-165
Identity = 308/362 (85.08%), Postives = 328/362 (90.61%), Query Frame = 0

Query: 1   MERLLYSSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPSI 60
           M+RLLYS SPTS  INPN  L+LPRF R IDSVK NFPL  RHGL PLG  SSK +KPS+
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRR-IDSVKLNFPLPTRHGLRPLGLLSSKVEKPSM 60

Query: 61  SSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVFA 120
             I CL SSN  +DSI  SDS  +SPKPD L+HFAGK+KVK SLFVLSALALILIQPVFA
Sbjct: 61  PLIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFA 120

Query: 121 PAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSI 180
           PAAFASFQN AKTGGPAA AVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 PAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIMG 240
           GC+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTI+VGVTLFVIG++G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLG 240

Query: 241 IREASEIRTPFVVALDNGECDVGIYETLEKPMVVG--NKKSKKLGFATFATGVVHGLQPD 300
           IREASE+RTP VV L+NGECDVGIY+TLEKPMV+G  NKK KK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 301 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWV 360
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW 
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of PI0021497 vs. NCBI nr
Match: XP_023529731.1 (uncharacterized protein LOC111792465 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 591.7 bits (1524), Expect = 4.4e-165
Identity = 307/362 (84.81%), Postives = 326/362 (90.06%), Query Frame = 0

Query: 1   MERLLYSSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPSI 60
           M+RLLYS SPTS  INPN  L+LPRF R IDS K NFPL  RHGL PLG  SSK +KPS+
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRR-IDSAKLNFPLPTRHGLRPLGLFSSKTEKPSM 60

Query: 61  SSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVFA 120
             I CL SSN  +DSI  SDS   SPKPD L+HFAGK+KVK SLFVLSALALILIQPVFA
Sbjct: 61  PLIHCLSSSNSSSDSISTSDSRAGSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFA 120

Query: 121 PAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSI 180
           PAAFASFQN AKTGGPAA AVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 PAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIMG 240
           GC+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTI+VGVTLFVIG++G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLG 240

Query: 241 IREASEIRTPFVVALDNGECDVGIYETLEKPMVVG--NKKSKKLGFATFATGVVHGLQPD 300
           IREASE+RTP VV L+NGECDVGIY+TLEKPMV+G  NKK KK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 301 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWV 360
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW 
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of PI0021497 vs. NCBI nr
Match: XP_022928346.1 (uncharacterized protein LOC111435202 [Cucurbita moschata])

HSP 1 Score: 589.3 bits (1518), Expect = 2.2e-164
Identity = 307/362 (84.81%), Postives = 325/362 (89.78%), Query Frame = 0

Query: 1   MERLLYSSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPSI 60
           M+RLLYS SPTS  INPN  L+LPRF R IDS K NFPL  RHGL PLG  SSK DKPS+
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRR-IDSAKLNFPLPTRHGLRPLGLFSSKVDKPSM 60

Query: 61  SSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFAGKEKVKSSLFVLSALALILIQPVFA 120
             I CL SSN  +DSI  S+S   SPKPD L+HFAGK+KVK SLFVLSALALILIQPVFA
Sbjct: 61  PLIHCLSSSNSLSDSISTSNSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFA 120

Query: 121 PAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSI 180
           PAAFASFQN AKTGGPAA AVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 PAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIMG 240
           GC+RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTI+VGVTLFVIGI+G
Sbjct: 181 GCSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGILG 240

Query: 241 IREASEIRTPFVVALDNGECDVGIYETLEKPMVVG--NKKSKKLGFATFATGVVHGLQPD 300
           IREASE+RTP VV L+NGECDVGIY+TLE PMV+G  NKK KK+GFATFATGVVHGLQPD
Sbjct: 241 IREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300

Query: 301 ALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWV 360
           ALMIVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTW 
Sbjct: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360

BLAST of PI0021497 vs. TAIR 10
Match: AT2G16800.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 367.9 bits (943), Expect = 9.6e-102
Identity = 201/340 (59.12%), Postives = 247/340 (72.65%), Query Frame = 0

Query: 39  LHIRHGLHPLGFTSSKN-DKPSISSIPCLFSSNLFTDS-----------IFHSDSPTASP 98
           LH  H  +   F SS+  +   ISSI C F  N   D+           I  S +  + P
Sbjct: 27  LHRLHSTNLSTFPSSRRLESRRISSISCFFRQNPSPDTSPGLNQSSNFLIASSQTDASKP 86

Query: 99  KPDFLKH----FAGKEKVKSSLFVL-SALALILIQPVFAPAAFASFQNGAKTGGPAAVAV 158
            P F++     F  ++ + + + +L SA+A +L+ P+  P AFASFQ    +GG  A  V
Sbjct: 87  NPGFIQRIVSSFEQRKTISAGMVILVSAIAALLLNPILVPPAFASFQTATNSGG--AAVV 146

Query: 159 GRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIGCNRMESAAVGALWGCGHDAG 218
           G +L+++E+L SAWTGFFAGCLHTLSGPDHLAALAPLSIG  RMESAAVGALWGCGHDAG
Sbjct: 147 GGKLLRTEVLTSAWTGFFAGCLHTLSGPDHLAALAPLSIGRTRMESAAVGALWGCGHDAG 206

Query: 219 QVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIMGIREASEIRTPFVVALDNGECD 278
           Q+IFGLLFLLLKD+LHIE++RTWGT +VG+TL VIG MGI+EASE+  P VV L+NGE D
Sbjct: 207 QLIFGLLFLLLKDRLHIEVIRTWGTRVVGLTLLVIGAMGIKEASEMPEPCVVTLENGETD 266

Query: 279 VGIYETLEKPMVVGNKKSKKLGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLLM 338
                  EK +     K KK+GFATFATG+VHGLQPDALM+VLPALALPSR+AGA+FL+M
Sbjct: 267 -------EKSL-----KKKKIGFATFATGIVHGLQPDALMMVLPALALPSRIAGASFLIM 326

Query: 339 FLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWVASL 362
           FL+GTVI+MGSYT FIGS SEALK++VPRITE+LTW +SL
Sbjct: 327 FLIGTVIAMGSYTVFIGSCSEALKEKVPRITEKLTWASSL 352

BLAST of PI0021497 vs. TAIR 10
Match: AT4G35080.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 340.9 bits (873), Expect = 1.3e-93
Identity = 205/369 (55.56%), Postives = 253/369 (68.56%), Query Frame = 0

Query: 1   MERLLY-SSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPS 60
           MERLL  SSS +S++ +   S   P   R+  S       H          + + +  PS
Sbjct: 1   MERLLQPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVSSISCNSSQIPS 60

Query: 61  I-SSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFA--GKEKVKS----SLFVLSALAL 120
           + + I    ++N F  S     S  + P P FL   A    E+ K+    ++ ++SA+A+
Sbjct: 61  LYTPIGSNTTNNSFNGS---PKSDESKPNPGFLTRIATSASEQRKTLSTGTVILISAVAV 120

Query: 121 ILIQPVFAPAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHL 180
           +L+ P+ AP AFASFQ  AK+G                L SAWTGF AGCLHTLSGPDHL
Sbjct: 121 LLLNPLLAPPAFASFQTAAKSG---------------WLTSAWTGFLAGCLHTLSGPDHL 180

Query: 181 AALAPLSIGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVT 240
           AALAPLSIG ++MESAAVGALWGCGHDAGQVIFGLLFLLLKD+LHIE+L+TWGT IVG+T
Sbjct: 181 AALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGLT 240

Query: 241 LFVIGIMGIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVV 300
           L +IG MGI+EASEI  P V      E D+ +  T ++ + +   K KK+GFATFATGVV
Sbjct: 241 LVIIGAMGIKEASEIPEPCVAL----ETDISMVSTEKEALPL--PKKKKIGFATFATGVV 300

Query: 301 HGLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRIT 360
           HGLQPDALMIVLPALALPSR+AG+AFL+MFLVGTVI+MGSYTAFIGS SEALK++VPRIT
Sbjct: 301 HGLQPDALMIVLPALALPSRLAGSAFLIMFLVGTVIAMGSYTAFIGSCSEALKEKVPRIT 345

Query: 361 ERLTWVASL 362
           E+LTWV+SL
Sbjct: 361 EKLTWVSSL 345

BLAST of PI0021497 vs. TAIR 10
Match: AT4G35080.3 (high-affinity nickel-transport family protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.5e-91
Identity = 177/259 (68.34%), Postives = 210/259 (81.08%), Query Frame = 0

Query: 103 SLFVLSALALILIQPVFAPAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGC 162
           ++ ++SA+A++L+ P+ AP AFASFQ  AK+G                L SAWTGF AGC
Sbjct: 132 TVILISAVAVLLLNPLLAPPAFASFQTAAKSG---------------WLTSAWTGFLAGC 191

Query: 163 LHTLSGPDHLAALAPLSIGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILR 222
           LHTLSGPDHLAALAPLSIG ++MESAAVGALWGCGHDAGQVIFGLLFLLLKD+LHIE+L+
Sbjct: 192 LHTLSGPDHLAALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQ 251

Query: 223 TWGTIIVGVTLFVIGIMGIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKL 282
           TWGT IVG+TL +IG MGI+EASEI  P V      E D+ +  T ++ + +   K KK+
Sbjct: 252 TWGTRIVGLTLVIIGAMGIKEASEIPEPCVAL----ETDISMVSTEKEALPL--PKKKKI 311

Query: 283 GFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSE 342
           GFATFATGVVHGLQPDALMIVLPALALPSR+AG+AFL+MFLVGTVI+MGSYTAFIGS SE
Sbjct: 312 GFATFATGVVHGLQPDALMIVLPALALPSRLAGSAFLIMFLVGTVIAMGSYTAFIGSCSE 369

Query: 343 ALKDRVPRITERLTWVASL 362
           ALK++VPRITE+LTWV+SL
Sbjct: 372 ALKEKVPRITEKLTWVSSL 369

BLAST of PI0021497 vs. TAIR 10
Match: AT4G35080.2 (high-affinity nickel-transport family protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.3e-76
Identity = 181/369 (49.05%), Postives = 225/369 (60.98%), Query Frame = 0

Query: 1   MERLLY-SSSPTSLNINPNSSLILPRFGRIIDSVKPNFPLHIRHGLHPLGFTSSKNDKPS 60
           MERLL  SSS +S++ +   S   P   R+  S       H          + + +  PS
Sbjct: 1   MERLLQPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVSSISCNSSQIPS 60

Query: 61  I-SSIPCLFSSNLFTDSIFHSDSPTASPKPDFLKHFA--GKEKVKS----SLFVLSALAL 120
           + + I    ++N F  S     S  + P P FL   A    E+ K+    ++ ++SA+A+
Sbjct: 61  LYTPIGSNTTNNSFNGS---PKSDESKPNPGFLTRIATSASEQRKTLSTGTVILISAVAV 120

Query: 121 ILIQPVFAPAAFASFQNGAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHL 180
           +L+ P+ AP AFASFQ  AK+G                L SAWTGF AGCLHTLSGPDHL
Sbjct: 121 LLLNPLLAPPAFASFQTAAKSG---------------WLTSAWTGFLAGCLHTLSGPDHL 180

Query: 181 AALAPLSIGCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVT 240
           AALAPLSIG ++MESAAVGALWGCGHDAGQVIFGLLFLLLKD+LHIE+L+TWGT IVG+T
Sbjct: 181 AALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGLT 240

Query: 241 LFVIGIMGIREASEIRTPFVVALDNGECDVGIYETLEKPMVVGNKKSKKLGFATFATGVV 300
           L +IG MGI+EASEI  P V      E D+ +  T ++ + +   K KK+GFATFATGVV
Sbjct: 241 LVIIGAMGIKEASEIPEPCVAL----ETDISMVSTEKEALPL--PKKKKIGFATFATGVV 300

Query: 301 HGLQPDALMIVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGSFSEALKDRVPRIT 360
           HGLQPDALMIVLPALALPSR                             EALK++VPRIT
Sbjct: 301 HGLQPDALMIVLPALALPSR-----------------------------EALKEKVPRIT 316

Query: 361 ERLTWVASL 362
           E+LTWV+SL
Sbjct: 361 EKLTWVSSL 316