PI0021431 (gene) Melon (PI 482460) v1

Overview
NamePI0021431
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPectinesterase
Locationchr01: 25080117 .. 25084258 (+)
RNA-Seq ExpressionPI0021431
SyntenyPI0021431
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCATTCTCTCTCCAATGGCTGCCGGAACCATCGCCACCGCCGCCGCATTCTACGTTCTAATCCTCATTTCCCTTTACATCACCACTTCTGCCTCCCCCACCGGTTCGGTTTGCAGCTCAACTCCAGACCCGTCGTACTGCAAATCGGCCCTCCCGAACCAAACAGGAAATGTGTACTCGTATGGACGGTCGTCATTCAGAAAGTCACTGTCGTCGTCACAGAAGTTCCTCCGGCTAGTAGAGAAGCACCTCCGATCCCGATCGTCGCTGACGGTGCCGGCGGTGAGGGCGTTGCAGGACTGCCAGCTGCTGGCGGGACTGAACATCGATTACTTGAGGACGTCGTTCCAGGCGGTGAACGCGACGAGCAGAGCATTGTCGGAGATGAAGGCGGACGATGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGACGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTTAAAAATGGCCTTTCTCAACCACTTGCTTCTGACACCAAGCTGTACTCTCTGTCTTTGGCCTTCTTCACAAAAGGTTTGTCATTATTTTTTATTAAAGGATGAGAAGAGAAATTTTGAAATAAAAAGAAAAAACTAGTTTTTTGAAATATTTTATATTCATTATTTTAAGGTTGAAAATGAAAACAAGTTGTTTAAGAAAAGATTTAAGTTTTTTGACCATTACAAAATCCTTTTGAAATTTATTTTACAACAAGAGTTTAATTAAAAACATTTTTTTTTTGTTTTTTCTCTAGTCAATTTAAACATAGGCTTAGTTTTTCAAAGATAATGTTTTTTAATAAAAATTTAGATTTCATACAATTTTCACAATTTGTAAATACAATGCTAATAAACATGATTTTTTTTTTGAAAACAAAAACACAAACTATATGGATGTAGGGATTAATTTTAAAATTTCTTAATAAAAAATAAAATCTTAAATATAGTTAAAATGGTAGGTTAACCAAAATAAAATGAGGGGATTTTCTCAATTTATCTATTTAATTAGGAGGGAGACAATGCAATTAGTTAGGTAATTATTTTGAGTTTAATCAAATGGAGGGGTAGATTTAGTTTTTTTGTTTTGTTTTTGTTTTTGTTTTTGTTGCTATATTTGGTTTTTATTAAATGTAATAGTAAGTCAAATTATTTTATATAAAAAAAAAAATCTTAGACACTAGTTTATAAGAGATTTGGATTGAATTTTTTTTTATGTGAGGTTTCTTTTGTGCCTCTATTAGAAAGTGATTTGGTAGTCAATTAGAATGCCATAGAAATAGACTAATATGACATGGAGAACATATAAATAAATAAATATACATATTTGGAGCCTTCTTGGTAATCCATTTGTTTTTTATTTTCAATAATTAATTTTTAAAAATCAAGTTTGTTTAGTAATTGATTCACTTTTTTGTTTTTTAAATAATAACAAATGAAAGAAAAATAAAAATGAAAAAGAAAAGACAAAGCCTTCAAAAATACATTTTGTTTTTTTTAAAAAAAACAATAAAATCAGTTATTTTTTAATAAACAAAATTTAAAAAAAATTAAACCAACAAAAAAAAAAACAAAAAAAAACAAAAAAAAAAAACAAAAATTAAAATGATTTAATGTTAAAAGTGAAAAGTTTTACCAATAAATTTGGTAGGCATTTATTCTTTAATTTTTCTATATTGATTTGATTGAATAACAAACTGTTATTGTTGTTAGGTGAGTTTAGTTGGAAGTTTTAGTAGGCTCTTAATTTTTAAGTATTGATTTGTTGAATTTGTAACCTATTTGTGTTTTTTTTTTCTCTAGGCTTAATTTAGAAAAAGAAAAAGAAAAAGAAAATATCTCCTTTTTTCTAATATGTATAAATTAAAATACTAAAATTATAGGAAATAGAAAATAATTTCATTTTCATTTATAAATTAAGTAAACAAAAACTAATTTGTTTTTTTATGAAAAGTTACTGTAAATGATATTTGATAGAAATAATTTACCTATAATCTAAAATTTTGTTATATTTTCTTCTTTTCTACTTATAAATTAATTTACCTGAAATTGTTGATTTCTTTTTCCGACAGGTTGGGTTCCGAAGAAGAAGAAAAAGCCGACATGGAAAGCCGCGGGAAGACAAGGCGGGTTTAGAAACGGACGAATGTCACTCAAAATGTCCAGCAGAACCCAAGCCATTTACGAGAGGGCGACCCGCCGGAATCTGCTGCAGACTGACGATGGCGGCGACGATCAGATTGCGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACAACTATCAATGAGGCCATTGCGGCGGCGCCGAACAACTCTGCTCCTACGGACGGGTATTTCTTGATCTTCGTGTCGGCGGGAGTTTATGAAGAGTATGTGTCAATTGCGAAGAACAAGAGATACTTGATGATGATCGGAGATGGCATTAATCAGACCGTCGTCACCGGTAATCGGAGCGTGGTTGACGGCTGGACGACTTTCAATTCCGCCACTTTTGGTAATTGCCTCCACCTTTTAAATGTTGTTAAGTAATTTGTTTTCAAAATAAAAGAAAATTTGTTAAATAGTAATTTTAAATTAGACCATAATTTTTTATAAACTTCTCCATGGATCTCCTCATTTTTTTTTTCTTTTTTCTTTTTTTAAGGGCTCACCAACTTTTCAGTTTAAAAGCTTCTAAATTTCTTGATTTTTTTTTTTTTTTATCAACTTTCCACTAAAGTTTTCAAAAAATAAGCCTTGAAAAGTAAGGAAATTGTTTCTTTGTTTTTTTTTTCCTCCTAAATTAAGCTTAGAAACGATAATTTTATGTTAGATTAAATTTAACTACTTAATTATAATTGACATAATTCTTTTCTTATTATTGTGCTAAAAAATAATTTGCATTCATAATTTGTTATTCATTATTAAATTAAATACGTTTTGAATTTATTATTATTATTTTTTTGTGTGCACTTGATTAGAAATAAAAGTGCTTTTGGAGAGGTAAAAACTAAAAATGAAGAGGAAACAAAGGGGAAAAAAAATTAAATGAGTATTAAAAATCCATTAATTCTTAAAATCTTATTTTTTCAGCAATAAAAATTGTTTTTAAGCATAGAACAGAATTAGTCAGATGTTATGATGAGAATGGGAGAAAATAATAGTGTAATTATTGTGTAAAATATTTTTAGATATAGCAAAATAGAAGAATAAATAATTTTAACAAAAAGTGGCGAAATTAATCCTTTGTTTTCGTTTTCATCACCAGCGGTGGTCGGACCTGGATTCGTAGCAGTGAACATGACATTCCGAAACACAGCCGGAGCCATAAAGCACCAAGCCGTCGCCGTCCGTAACGGCGCCGACCTATCAACATTCTACCTCTGTAGCTTCGAAGCATACCAAGACACCTTATACACTCATTCCCTCCGCCAATTCTACCGCGACTGCGACATCTACGGCACAGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAGAACTGCAACATTTACCCACGCCTCCCAATGTCCAACCAGTTCAACGCCATCACCGCCCAAGGCCGGACCGACCCAAATCAGAACACCGGCACCTCGATCTACAATTGCCGAATAACAGCCGCCGACGATCTGGCGAACAACAGCGGCGCCGGCGTGAAGACGTTCCTGGGACGGCCATGGAAGGAGTACTCGAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTAATCCGGCGGGATGGAGAGCTTGGGATGGTGATTTTGCTTTGAATACTTCGTATTATGCGGAATTTGGGAATTTTGGGCCGGGATCTAACACGTCAGAGAGGGTTAATTGGCCGGGATTTCATTTGATTAACGATACTGATGCTGGGAATTTCACAGCCGGGAATTTTGTGTTGGCCGATGATTGGCTGCCGCAGACTGGCGTTCCATATACCAGCGGCCTCACGGAGTAGAATCAGGACAGAATTGTAATTTTCGTATAGTACTTTAATTATTTTTTTTTTTATAAATTCCCAAGCCAAAGTGTATAGCTATGGGATGAAATATGATTGATTCTTTTGTACGGTCATTTATTTTCAAATATCCATAAATATATATATTCCATTTTAGTTCAA

mRNA sequence

CTCCATTCTCTCTCCAATGGCTGCCGGAACCATCGCCACCGCCGCCGCATTCTACGTTCTAATCCTCATTTCCCTTTACATCACCACTTCTGCCTCCCCCACCGGTTCGGTTTGCAGCTCAACTCCAGACCCGTCGTACTGCAAATCGGCCCTCCCGAACCAAACAGGAAATGTGTACTCGTATGGACGGTCGTCATTCAGAAAGTCACTGTCGTCGTCACAGAAGTTCCTCCGGCTAGTAGAGAAGCACCTCCGATCCCGATCGTCGCTGACGGTGCCGGCGGTGAGGGCGTTGCAGGACTGCCAGCTGCTGGCGGGACTGAACATCGATTACTTGAGGACGTCGTTCCAGGCGGTGAACGCGACGAGCAGAGCATTGTCGGAGATGAAGGCGGACGATGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGACGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTTAAAAATGGCCTTTCTCAACCACTTGCTTCTGACACCAAGCTGTACTCTCTGTCTTTGGCCTTCTTCACAAAAGGTTGGGTTCCGAAGAAGAAGAAAAAGCCGACATGGAAAGCCGCGGGAAGACAAGGCGGGTTTAGAAACGGACGAATGTCACTCAAAATGTCCAGCAGAACCCAAGCCATTTACGAGAGGGCGACCCGCCGGAATCTGCTGCAGACTGACGATGGCGGCGACGATCAGATTGCGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACAACTATCAATGAGGCCATTGCGGCGGCGCCGAACAACTCTGCTCCTACGGACGGGTATTTCTTGATCTTCGTGTCGGCGGGAGTTTATGAAGAGTATGTGTCAATTGCGAAGAACAAGAGATACTTGATGATGATCGGAGATGGCATTAATCAGACCGTCGTCACCGGTAATCGGAGCGTGGTTGACGGCTGGACGACTTTCAATTCCGCCACTTTTGCGGTGGTCGGACCTGGATTCGTAGCAGTGAACATGACATTCCGAAACACAGCCGGAGCCATAAAGCACCAAGCCGTCGCCGTCCGTAACGGCGCCGACCTATCAACATTCTACCTCTGTAGCTTCGAAGCATACCAAGACACCTTATACACTCATTCCCTCCGCCAATTCTACCGCGACTGCGACATCTACGGCACAGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAGAACTGCAACATTTACCCACGCCTCCCAATGTCCAACCAGTTCAACGCCATCACCGCCCAAGGCCGGACCGACCCAAATCAGAACACCGGCACCTCGATCTACAATTGCCGAATAACAGCCGCCGACGATCTGGCGAACAACAGCGGCGCCGGCGTGAAGACGTTCCTGGGACGGCCATGGAAGGAGTACTCGAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTAATCCGGCGGGATGGAGAGCTTGGGATGGTGATTTTGCTTTGAATACTTCGTATTATGCGGAATTTGGGAATTTTGGGCCGGGATCTAACACGTCAGAGAGGGTTAATTGGCCGGGATTTCATTTGATTAACGATACTGATGCTGGGAATTTCACAGCCGGGAATTTTGTGTTGGCCGATGATTGGCTGCCGCAGACTGGCGTTCCATATACCAGCGGCCTCACGGAGTAGAATCAGGACAGAATTGTAATTTTCGTATAGTACTTTAATTATTTTTTTTTTTATAAATTCCCAAGCCAAAGTGTATAGCTATGGGATGAAATATGATTGATTCTTTTGTACGGTCATTTATTTTCAAATATCCATAAATATATATATTCCATTTTAGTTCAA

Coding sequence (CDS)

ATGGCTGCCGGAACCATCGCCACCGCCGCCGCATTCTACGTTCTAATCCTCATTTCCCTTTACATCACCACTTCTGCCTCCCCCACCGGTTCGGTTTGCAGCTCAACTCCAGACCCGTCGTACTGCAAATCGGCCCTCCCGAACCAAACAGGAAATGTGTACTCGTATGGACGGTCGTCATTCAGAAAGTCACTGTCGTCGTCACAGAAGTTCCTCCGGCTAGTAGAGAAGCACCTCCGATCCCGATCGTCGCTGACGGTGCCGGCGGTGAGGGCGTTGCAGGACTGCCAGCTGCTGGCGGGACTGAACATCGATTACTTGAGGACGTCGTTCCAGGCGGTGAACGCGACGAGCAGAGCATTGTCGGAGATGAAGGCGGACGATGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGACGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTTAAAAATGGCCTTTCTCAACCACTTGCTTCTGACACCAAGCTGTACTCTCTGTCTTTGGCCTTCTTCACAAAAGGTTGGGTTCCGAAGAAGAAGAAAAAGCCGACATGGAAAGCCGCGGGAAGACAAGGCGGGTTTAGAAACGGACGAATGTCACTCAAAATGTCCAGCAGAACCCAAGCCATTTACGAGAGGGCGACCCGCCGGAATCTGCTGCAGACTGACGATGGCGGCGACGATCAGATTGCGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACAACTATCAATGAGGCCATTGCGGCGGCGCCGAACAACTCTGCTCCTACGGACGGGTATTTCTTGATCTTCGTGTCGGCGGGAGTTTATGAAGAGTATGTGTCAATTGCGAAGAACAAGAGATACTTGATGATGATCGGAGATGGCATTAATCAGACCGTCGTCACCGGTAATCGGAGCGTGGTTGACGGCTGGACGACTTTCAATTCCGCCACTTTTGCGGTGGTCGGACCTGGATTCGTAGCAGTGAACATGACATTCCGAAACACAGCCGGAGCCATAAAGCACCAAGCCGTCGCCGTCCGTAACGGCGCCGACCTATCAACATTCTACCTCTGTAGCTTCGAAGCATACCAAGACACCTTATACACTCATTCCCTCCGCCAATTCTACCGCGACTGCGACATCTACGGCACAGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAGAACTGCAACATTTACCCACGCCTCCCAATGTCCAACCAGTTCAACGCCATCACCGCCCAAGGCCGGACCGACCCAAATCAGAACACCGGCACCTCGATCTACAATTGCCGAATAACAGCCGCCGACGATCTGGCGAACAACAGCGGCGCCGGCGTGAAGACGTTCCTGGGACGGCCATGGAAGGAGTACTCGAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTAATCCGGCGGGATGGAGAGCTTGGGATGGTGATTTTGCTTTGAATACTTCGTATTATGCGGAATTTGGGAATTTTGGGCCGGGATCTAACACGTCAGAGAGGGTTAATTGGCCGGGATTTCATTTGATTAACGATACTGATGCTGGGAATTTCACAGCCGGGAATTTTGTGTTGGCCGATGATTGGCTGCCGCAGACTGGCGTTCCATATACCAGCGGCCTCACGGAGTAG

Protein sequence

MAAGTIATAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRALSEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGLTE
Homology
BLAST of PI0021431 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 3.6e-201
Identity = 354/573 (61.78%), Postives = 434/573 (75.74%), Query Frame = 0

Query: 13  YVLILISLYI------TTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLS 72
           ++LI +S ++      + + S + ++C +TPDP YCKS  P+  GNV  YG  S RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 73  SSQKFLRLVEKHLRSRSSLTVPAV-RALQDCQLLAGLNIDYLRTSFQAVNATS-----RA 132
            S+KF+R V+++++  + L+ PAV RALQDC+ LAGL +DYL TSF+ VN TS     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 133 LSEMKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSQPLASDTKLYSLSLAF 192
           LS  KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++ PL +DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 193 FTKGWVPKKKKKPTWKAAGRQGG----------FRNGRMSLKMSSRTQAIYERATRRNLL 252
           FTKGWVPKKKK+  +  A  + G          FRNG + LKM+ +T+A+YE  +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 253 QTDDGGD----DQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVY 312
             D  GD      + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 313 EEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNT 372
           EEY+SIAKNKRY+MMIGDGINQTVVTGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 373 AGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 432
           AG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 433 FQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGR 492
           FQNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   VKT+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGR 485

Query: 493 PWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFH 552
           PWKEYSRTVYMQS++D  + P GWR W+GDFAL+T YYAE+ N GPGSNT+ RV WPG+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 553 LINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 557
           +IN TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of PI0021431 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 680.2 bits (1754), Expect = 1.9e-194
Identity = 353/567 (62.26%), Postives = 430/567 (75.84%), Query Frame = 0

Query: 12  FYVLILISLYITTSASPT-------GSVCSSTPDPSYCKSALPNQT-GNVYSYGRSSFRK 71
           F V + +SL     AS T        ++C +TPDP +CKS  P  + G+V  YGR S RK
Sbjct: 7   FLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRK 66

Query: 72  SLSSSQKFLRLVEKHL-RSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNAT--SRA 131
           SL+ S+KF R ++++L R+ + L+  AV ALQDC+ LA L  DYL TSF+ VN T  S+ 
Sbjct: 67  SLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKT 126

Query: 132 LSEMKADDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 191
           LS  KAD++Q+LLSA LTN+QTCLDGI  A + SW+++NG++ PL +DTKL+S+SLA FT
Sbjct: 127 LSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFT 186

Query: 192 KGWVPKKKK----------KPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQT 251
           KGWVPKKKK          K T         FRNG + LKM+  T+A+YE  +RR L   
Sbjct: 187 KGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD- 246

Query: 252 DDGGDDQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSI 311
           DD   + + V DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V++GVYEE V I
Sbjct: 247 DDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVI 306

Query: 312 AKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKH 371
           AKNKRYLMMIGDGIN+TVVTGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KH
Sbjct: 307 AKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKH 366

Query: 372 QAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI 431
           QAVA+R+ ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+
Sbjct: 367 QAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNL 426

Query: 432 YPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYS 491
           YPR PM NQFNAITAQGRTDPNQNTG SI+NC I  ADDL  +S   VKT+LGRPWKEYS
Sbjct: 427 YPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYS 486

Query: 492 RTVYMQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTD 551
           RTV+MQS++D+++ P GWR W+GDFAL+T YYAE+ N G GS+T++RV WPG+H+IN TD
Sbjct: 487 RTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTD 546

Query: 552 AGNFTAGNFVLADDWLPQTGVPYTSGL 557
           A NFT  NF+L D W+ Q+GVPY SGL
Sbjct: 547 ANNFTVENFLLGDGWMVQSGVPYISGL 571

BLAST of PI0021431 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 655.6 bits (1690), Expect = 5.0e-187
Identity = 322/537 (59.96%), Postives = 411/537 (76.54%), Query Frame = 0

Query: 25  SASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLRSRSS 84
           +A+   ++C   PDPSYC+S LPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 85  LTVPA-VRALQDCQLLAGLNIDYLRTSFQAVNATSRALSEMKADDVQSLLSAILTNQQTC 144
           +   + V AL+DC+ LA L +DYL +S Q  ++T + LS  +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 145 LDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKK-KPTWKAAGRQG--- 204
           L+G+K+TA     +NGLS  L +DTKLY +SLA F+KGWVP++++ +P W+   R     
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 205 GFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTINEA 264
           GFRNG++ LKM+ R +A+Y   TRR LLQ+D    D + V DIV V Q+G+GNFTTIN A
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAA 270

Query: 265 IAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWT 324
           IAAAPN +  ++GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+TGNRSVVDGWT
Sbjct: 271 IAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWT 330

Query: 325 TFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHS 384
           TFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHS
Sbjct: 331 TFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHS 390

Query: 385 LRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIY 444
           LRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+I+
Sbjct: 391 LRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIH 450

Query: 445 NCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTS 504
            C I  ADDLA  S   VKT+LGRPWKEYSRTV MQ+++D  + P+GW AW GDFAL+T 
Sbjct: 451 GCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 510

Query: 505 YYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 557
           YYAE+ N GPGS+T+ RV WPG+H+IN TDA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 511 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of PI0021431 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 2.4e-136
Identity = 262/551 (47.55%), Postives = 354/551 (64.25%), Query Frame = 0

Query: 19  SLYITTSASPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLV 78
           SL  + S SP+   C STP P  C+   +A+ +   + Y YG+ + ++ L  + +  +++
Sbjct: 70  SLPPSQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 129

Query: 79  EKHLR----SRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRALSEMKADDVQS 138
             + R       S T   + A+ DC  L+ L+++YL T    +  T++ ++    + V S
Sbjct: 130 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNS 189

Query: 139 LLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKKPT 198
           LLS ++TNQQTCLDG+      ++    +  P+ + T+LYS+SL   +       K+   
Sbjct: 190 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKA 249

Query: 199 WKAAGRQGG---FRNGRMSLKMSSRTQAIYE---RATRRNLLQTDDGGDDQIAVRDIVVV 258
            K     GG   +R    +L    R     +   R T RNL +  +     I V   V+V
Sbjct: 250 SKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIV 309

Query: 259 SQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQ 318
               S NFTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+ +  NK+ LM++GDGIN+
Sbjct: 310 GPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINK 369

Query: 319 TVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYL 378
           T++TGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+FY 
Sbjct: 370 TIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYR 429

Query: 379 CSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQ 438
           CSFE YQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM+ Q NAITA 
Sbjct: 430 CSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAH 489

Query: 439 GRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPA 498
           GR DPNQNTG SI NC I AA DLA    + + TFLGRPWK YSRTV+MQS++ D++ P 
Sbjct: 490 GRLDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPV 549

Query: 499 GWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWL 557
           GW  W+G   L+T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  NF + D WL
Sbjct: 550 GWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWL 609

BLAST of PI0021431 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 2.4e-136
Identity = 264/545 (48.44%), Postives = 348/545 (63.85%), Query Frame = 0

Query: 25  SASPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLR- 84
           S  P    C STP P  C+   SA+ +   + Y YG+ + ++ L  +++  +++ +  + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 85  ---SRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRALSEMKADDVQSLLSAIL 144
                 + TV  V A+ DC  LA L+++YL T  + + A +  ++    D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKA-AELMTAALVDRVTSLLGGVV 199

Query: 145 TNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKKPTWKAAGR 204
           TNQQTCLDG+      ++    +  PL + T+LYS+SL   +       K+    K    
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIF 259

Query: 205 QGGFRNGRMSL----KMSSRT--QAIYERATRRNLLQTDDGGDDQIAVRDIVVVSQDGSG 264
            GG +  R  L    K+  +T  +    R   RNL +  +     I VR+ V V    + 
Sbjct: 260 GGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETD 319

Query: 265 NFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVVTGN 324
           NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T+++GN
Sbjct: 320 NFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGN 379

Query: 325 RSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAY 384
            S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFE Y
Sbjct: 380 HSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGY 439

Query: 385 QDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPN 444
           QDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM+NQ NA+TA GRTDPN
Sbjct: 440 QDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPN 499

Query: 445 QNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWD 504
           Q TG SI NC I AA DLA +      TFLGRPWK YSRTVY+QS++ D++ P GW  W+
Sbjct: 500 QKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWN 559

Query: 505 GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVP 557
           G   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT +P
Sbjct: 560 GTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIP 619

BLAST of PI0021431 vs. ExPASy TrEMBL
Match: A0A5D3D3H4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001320 PE=3 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 548/560 (97.86%), Postives = 553/560 (98.75%), Query Frame = 0

Query: 1   MAAGTIATAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSS 60
           MAAGT  TAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSS
Sbjct: 1   MAAGT--TAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSS 60

Query: 61  FRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRA 120
           FRKSLSSSQKFLRLVEKHLRSRSSLTV AVRAL+DCQLLAGLNIDYLRTSFQAVNATSRA
Sbjct: 61  FRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRA 120

Query: 121 LSEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTK 180
           L+EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTK
Sbjct: 121 LTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTK 180

Query: 181 GWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDD--GGDDQIA 240
           GWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYE ATRRNLLQTDD  G DDQIA
Sbjct: 181 GWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIA 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM 300
           VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM
Sbjct: 241 VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM 300

Query: 301 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGA
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ
Sbjct: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480
           FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480

Query: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540
           DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF
Sbjct: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540

Query: 541 VLADDWLPQTGVPYTSGLTE 559
           VLADDWLPQTGVPYTSGLTE
Sbjct: 541 VLADDWLPQTGVPYTSGLTE 558

BLAST of PI0021431 vs. ExPASy TrEMBL
Match: A0A1S3BSI2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 548/560 (97.86%), Postives = 553/560 (98.75%), Query Frame = 0

Query: 1   MAAGTIATAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSS 60
           MAAGT  TAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSS
Sbjct: 1   MAAGT--TAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSS 60

Query: 61  FRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRA 120
           FRKSLSSSQKFLRLVEKHLRSRSSLTV AVRAL+DCQLLAGLNIDYLRTSFQAVNATSRA
Sbjct: 61  FRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRA 120

Query: 121 LSEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTK 180
           L+EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTK
Sbjct: 121 LTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTK 180

Query: 181 GWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDD--GGDDQIA 240
           GWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYE ATRRNLLQTDD  G DDQIA
Sbjct: 181 GWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIA 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM 300
           VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM
Sbjct: 241 VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM 300

Query: 301 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGA
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ
Sbjct: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480
           FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480

Query: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540
           DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF
Sbjct: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540

Query: 541 VLADDWLPQTGVPYTSGLTE 559
           VLADDWLPQTGVPYTSGLTE
Sbjct: 541 VLADDWLPQTGVPYTSGLTE 558

BLAST of PI0021431 vs. ExPASy TrEMBL
Match: A0A0A0KB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 1.0e-307
Identity = 539/560 (96.25%), Postives = 546/560 (97.50%), Query Frame = 0

Query: 1   MAAGTIATAAAFYV-LILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60
           MAAGT ATAAAFYV LILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSR 120
           SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRAL+DC LLAGLNIDYL+TSFQ VN TSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTTSR 120

Query: 121 ALSEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180
            L+EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT
Sbjct: 121 VLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGG-DDQIA 240
           KGWVPKKKK+PTWKAAGRQGGFRNGRMSLKMSSRTQAIYE+ATRRNLLQTDDGG DDQI 
Sbjct: 181 KGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQIK 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM 300
           VRDIVVVSQDGSGNFTTINEAIAAA NNSAPTDGYFLIFVSAGVYEEYV +AKNKRYLMM
Sbjct: 241 VRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYLMM 300

Query: 301 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ
Sbjct: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480
           FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNS AGVKTFLGRPWKEYSRTVYMQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVYMQSFM 480

Query: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540
           DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERV W GFHLINDTDAGNFTAGNF
Sbjct: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNFTAGNF 540

Query: 541 VLADDWLPQTGVPYTSGLTE 559
           VLADDWLPQTGVPY SGLTE
Sbjct: 541 VLADDWLPQTGVPYDSGLTE 560

BLAST of PI0021431 vs. ExPASy TrEMBL
Match: A0A6J1GQE6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 1.7e-291
Identity = 506/556 (91.01%), Postives = 528/556 (94.96%), Query Frame = 0

Query: 8   TAAAFYVLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFR 67
           T AAFY L+LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVYSYGRSS R
Sbjct: 7   TTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVR 66

Query: 68  KSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRALS 127
           KSLSSSQKFLRLVEK+LRSRSSLTV AVRAL+DCQLLAGLNIDYL TSF+ VN+TS  L 
Sbjct: 67  KSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLP 126

Query: 128 EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGW 187
           EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLAFFTKGW
Sbjct: 127 EMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGW 186

Query: 188 VPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGGDDQIAVRDI 247
           VPKKKKKPTW +AGRQGGF NGRMSLKMSSRT+AIYE ATRRNLLQT+DGGDDQI+VRDI
Sbjct: 187 VPKKKKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQISVRDI 246

Query: 248 VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDG 307
           VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV+IAKNKRYLMMIGDG
Sbjct: 247 VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDG 306

Query: 308 INQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 367
           INQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRN AGAIKHQAVAVRN ADLST
Sbjct: 307 INQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNAAGAIKHQAVAVRNSADLST 366

Query: 368 FYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAI 427
           FYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+NQFNAI
Sbjct: 367 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAI 426

Query: 428 TAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLI 487
           TAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQSFMDDLI
Sbjct: 427 TAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLI 486

Query: 488 NPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLAD 547
           NPAGWRAWDGDFALNT YYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTAGNF++AD
Sbjct: 487 NPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAGNFLMAD 546

Query: 548 DWLPQTGVPYTSGLTE 559
           DW+PQTGVPYTSGLTE
Sbjct: 547 DWVPQTGVPYTSGLTE 562

BLAST of PI0021431 vs. ExPASy TrEMBL
Match: A0A6J1JVJ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 2.8e-289
Identity = 502/556 (90.29%), Postives = 526/556 (94.60%), Query Frame = 0

Query: 8   TAAAFYVLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFR 67
           T  AFY L+LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVYSYGRSS R
Sbjct: 7   TTPAFYYLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVR 66

Query: 68  KSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRALS 127
           KSLSSSQKFLRLVEK+LRSRSSLTV AVR+L+DCQLLAGLNIDYL TSF+ VN+TS  L 
Sbjct: 67  KSLSSSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRTVNSTSWTLP 126

Query: 128 EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGW 187
           EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PL +DTKLYS+SLAFFTKGW
Sbjct: 127 EMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSMSLAFFTKGW 186

Query: 188 VPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGGDDQIAVRDI 247
           VPKKKKK TW +AGRQGGF NGRMS KMSSRT+AIYE ATRRNLLQTDDGGDDQI+VRDI
Sbjct: 187 VPKKKKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQTDDGGDDQISVRDI 246

Query: 248 VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDG 307
           VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV+IAKNKRYLMMIGDG
Sbjct: 247 VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDG 306

Query: 308 INQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 367
           INQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN ADLST
Sbjct: 307 INQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLST 366

Query: 368 FYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAI 427
           FYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+NQFNAI
Sbjct: 367 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAI 426

Query: 428 TAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLI 487
           TAQGRTDPNQNTGTSI+NCRITAADDLANN+GA V TFLGRPWKEYSRTVYMQSFM+DLI
Sbjct: 427 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGARVNTFLGRPWKEYSRTVYMQSFMNDLI 486

Query: 488 NPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLAD 547
           NPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTAGNF++AD
Sbjct: 487 NPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAGNFLMAD 546

Query: 548 DWLPQTGVPYTSGLTE 559
           DW+PQTGVPYTSGLTE
Sbjct: 547 DWVPQTGVPYTSGLTE 562

BLAST of PI0021431 vs. NCBI nr
Match: XP_008451544.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KAA0046659.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa] >TYK18194.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 548/560 (97.86%), Postives = 553/560 (98.75%), Query Frame = 0

Query: 1   MAAGTIATAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSS 60
           MAAGT  TAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSS
Sbjct: 1   MAAGT--TAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSS 60

Query: 61  FRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRA 120
           FRKSLSSSQKFLRLVEKHLRSRSSLTV AVRAL+DCQLLAGLNIDYLRTSFQAVNATSRA
Sbjct: 61  FRKSLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRA 120

Query: 121 LSEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTK 180
           L+EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTK
Sbjct: 121 LTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTK 180

Query: 181 GWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDD--GGDDQIA 240
           GWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYE ATRRNLLQTDD  G DDQIA
Sbjct: 181 GWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIA 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM 300
           VRDIVVVSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM
Sbjct: 241 VRDIVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM 300

Query: 301 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGA
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ
Sbjct: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480
           FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480

Query: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540
           DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF
Sbjct: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540

Query: 541 VLADDWLPQTGVPYTSGLTE 559
           VLADDWLPQTGVPYTSGLTE
Sbjct: 541 VLADDWLPQTGVPYTSGLTE 558

BLAST of PI0021431 vs. NCBI nr
Match: XP_004136013.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1 hypothetical protein Csa_016133 [Cucumis sativus])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.1e-307
Identity = 539/560 (96.25%), Postives = 546/560 (97.50%), Query Frame = 0

Query: 1   MAAGTIATAAAFYV-LILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60
           MAAGT ATAAAFYV LILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSR 120
           SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRAL+DC LLAGLNIDYL+TSFQ VN TSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTTSR 120

Query: 121 ALSEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180
            L+EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT
Sbjct: 121 VLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGG-DDQIA 240
           KGWVPKKKK+PTWKAAGRQGGFRNGRMSLKMSSRTQAIYE+ATRRNLLQTDDGG DDQI 
Sbjct: 181 KGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQIK 240

Query: 241 VRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMM 300
           VRDIVVVSQDGSGNFTTINEAIAAA NNSAPTDGYFLIFVSAGVYEEYV +AKNKRYLMM
Sbjct: 241 VRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYLMM 300

Query: 301 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ
Sbjct: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480
           FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNS AGVKTFLGRPWKEYSRTVYMQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVYMQSFM 480

Query: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540
           DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERV W GFHLINDTDAGNFTAGNF
Sbjct: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNFTAGNF 540

Query: 541 VLADDWLPQTGVPYTSGLTE 559
           VLADDWLPQTGVPY SGLTE
Sbjct: 541 VLADDWLPQTGVPYDSGLTE 560

BLAST of PI0021431 vs. NCBI nr
Match: XP_038896831.1 (probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida])

HSP 1 Score: 1061.6 bits (2744), Expect = 2.3e-306
Identity = 534/561 (95.19%), Postives = 544/561 (96.97%), Query Frame = 0

Query: 1   MAAGTI---ATAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYG 60
           MAAGTI     AAAFY LILISLYITTSA+PTGS CSST DPSYCKS LPNQTGNVYSYG
Sbjct: 1   MAAGTIPAATAAAAFYFLILISLYITTSAAPTGSECSSTRDPSYCKSVLPNQTGNVYSYG 60

Query: 61  RSSFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNAT 120
           RSSFRKSLS+SQKFLRLVEKHLRSRSSLTVPAVRAL+DCQLLAGLNIDYL TSF+AVN+T
Sbjct: 61  RSSFRKSLSASQKFLRLVEKHLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120

Query: 121 SRALSEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAF 180
           SR L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAF
Sbjct: 121 SRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGGDDQI 240
           FTKGWVPKKKKKPTWKAAGR GGFRNGRMSLKMSSR +AIYE ATRRNLLQ DDGGDDQI
Sbjct: 181 FTKGWVPKKKKKPTWKAAGRHGGFRNGRMSLKMSSRAKAIYETATRRNLLQADDGGDDQI 240

Query: 241 AVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLM 300
           AVRDIVVVSQDGSGNFTTINEA+AAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLM
Sbjct: 241 AVRDIVVVSQDGSGNFTTINEAVAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLM 300

Query: 301 MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG 360
           MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG
Sbjct: 301 MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG 360

Query: 361 ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN 420
           ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN
Sbjct: 361 ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN 420

Query: 421 QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSF 480
           QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANN+GAGVKTFLGRPWKEYSRTVYMQSF
Sbjct: 421 QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNTGAGVKTFLGRPWKEYSRTVYMQSF 480

Query: 481 MDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN 540
           MDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN
Sbjct: 481 MDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN 540

Query: 541 FVLADDWLPQTGVPYTSGLTE 559
           FVLADDWLPQTGVPYTSGLT+
Sbjct: 541 FVLADDWLPQTGVPYTSGLTD 561

BLAST of PI0021431 vs. NCBI nr
Match: KAG7014346.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1013.1 bits (2618), Expect = 9.5e-292
Identity = 507/556 (91.19%), Postives = 529/556 (95.14%), Query Frame = 0

Query: 8   TAAAFYVLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFR 67
           T AAFY L+LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVYSYGRSS R
Sbjct: 7   TTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVR 66

Query: 68  KSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRALS 127
           KSLSSSQKFLRLVEK+LRSRSSLTV AVRAL+DCQLLAGLNIDYL TSF+ VN+TS  L 
Sbjct: 67  KSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLP 126

Query: 128 EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGW 187
           EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLAFFTKGW
Sbjct: 127 EMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGW 186

Query: 188 VPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGGDDQIAVRDI 247
           VPKKKKKPTW +AGRQGGF NGRMSLKMSSRT+AIYE ATRRNLLQTDDGGDDQI+VRDI
Sbjct: 187 VPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQISVRDI 246

Query: 248 VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDG 307
           VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV+IAKNKRYLMMIGDG
Sbjct: 247 VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDG 306

Query: 308 INQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 367
           INQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN ADLST
Sbjct: 307 INQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLST 366

Query: 368 FYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAI 427
           FYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+NQFNAI
Sbjct: 367 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAI 426

Query: 428 TAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLI 487
           TAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQSFMDDLI
Sbjct: 427 TAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLI 486

Query: 488 NPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLAD 547
           NPAGWRAWDGDFALNT YYAEFGNFGPGSNT+ERVNW GFHLI+DTDAGNFTAGNF++AD
Sbjct: 487 NPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAGNFLMAD 546

Query: 548 DWLPQTGVPYTSGLTE 559
           DW+PQTGVPYTSGLTE
Sbjct: 547 DWVPQTGVPYTSGLTE 562

BLAST of PI0021431 vs. NCBI nr
Match: KAG6575804.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.2e-291
Identity = 507/556 (91.19%), Postives = 529/556 (95.14%), Query Frame = 0

Query: 8   TAAAFYVLILISLYIT-----TSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFR 67
           T AAFY L+LISLYIT     TSA+PTGSVCSS PDPSYCKSALPNQTGNVYSYGRSS R
Sbjct: 7   TTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVR 66

Query: 68  KSLSSSQKFLRLVEKHLRSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRALS 127
           KSLSSSQKFLRLVEK+LRSRSSLTV AVRAL+DCQLLAGLNIDYL TSF+ VN+TS  L 
Sbjct: 67  KSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLP 126

Query: 128 EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGW 187
           EMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLS PLA+DTKLYS+SLAFFTKGW
Sbjct: 127 EMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGW 186

Query: 188 VPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGGDDQIAVRDI 247
           VPKKKKKPTW +AGRQGGF NGRMSLKMSSRT+AIYE ATRRNLLQT+DGGDDQI+VRDI
Sbjct: 187 VPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQISVRDI 246

Query: 248 VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDG 307
           VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV AGVYEEYV+IAKNKRYLMMIGDG
Sbjct: 247 VVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDG 306

Query: 308 INQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 367
           INQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN ADLST
Sbjct: 307 INQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLST 366

Query: 368 FYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAI 427
           FYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+NQFNAI
Sbjct: 367 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAI 426

Query: 428 TAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLI 487
           TAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQSFMDDLI
Sbjct: 427 TAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLI 486

Query: 488 NPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLAD 547
           NPAGWRAWDGDFALNT YYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTAGNF++AD
Sbjct: 487 NPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAGNFLMAD 546

Query: 548 DWLPQTGVPYTSGLTE 559
           DW+PQTGVPYTSGLTE
Sbjct: 547 DWVPQTGVPYTSGLTE 562

BLAST of PI0021431 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 702.6 bits (1812), Expect = 2.6e-202
Identity = 354/573 (61.78%), Postives = 434/573 (75.74%), Query Frame = 0

Query: 13  YVLILISLYI------TTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLS 72
           ++LI +S ++      + + S + ++C +TPDP YCKS  P+  GNV  YG  S RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 73  SSQKFLRLVEKHLRSRSSLTVPAV-RALQDCQLLAGLNIDYLRTSFQAVNATS-----RA 132
            S+KF+R V+++++  + L+ PAV RALQDC+ LAGL +DYL TSF+ VN TS     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 133 LSEMKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSQPLASDTKLYSLSLAF 192
           LS  KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++ PL +DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 193 FTKGWVPKKKKKPTWKAAGRQGG----------FRNGRMSLKMSSRTQAIYERATRRNLL 252
           FTKGWVPKKKK+  +  A  + G          FRNG + LKM+ +T+A+YE  +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 253 QTDDGGD----DQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVY 312
             D  GD      + + DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+AG+Y
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 313 EEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNT 372
           EEY+SIAKNKRY+MMIGDGINQTVVTGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 373 AGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 432
           AG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 433 FQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGR 492
           FQNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   VKT+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGR 485

Query: 493 PWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFH 552
           PWKEYSRTVYMQS++D  + P GWR W+GDFAL+T YYAE+ N GPGSNT+ RV WPG+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 553 LINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 557
           +IN TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of PI0021431 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 680.2 bits (1754), Expect = 1.4e-195
Identity = 353/567 (62.26%), Postives = 430/567 (75.84%), Query Frame = 0

Query: 12  FYVLILISLYITTSASPT-------GSVCSSTPDPSYCKSALPNQT-GNVYSYGRSSFRK 71
           F V + +SL     AS T        ++C +TPDP +CKS  P  + G+V  YGR S RK
Sbjct: 7   FLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRK 66

Query: 72  SLSSSQKFLRLVEKHL-RSRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNAT--SRA 131
           SL+ S+KF R ++++L R+ + L+  AV ALQDC+ LA L  DYL TSF+ VN T  S+ 
Sbjct: 67  SLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKT 126

Query: 132 LSEMKADDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 191
           LS  KAD++Q+LLSA LTN+QTCLDGI  A + SW+++NG++ PL +DTKL+S+SLA FT
Sbjct: 127 LSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFT 186

Query: 192 KGWVPKKKK----------KPTWKAAGRQGGFRNGRMSLKMSSRTQAIYERATRRNLLQT 251
           KGWVPKKKK          K T         FRNG + LKM+  T+A+YE  +RR L   
Sbjct: 187 KGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD- 246

Query: 252 DDGGDDQIAVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSI 311
           DD   + + V DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V++GVYEE V I
Sbjct: 247 DDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVI 306

Query: 312 AKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKH 371
           AKNKRYLMMIGDGIN+TVVTGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KH
Sbjct: 307 AKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKH 366

Query: 372 QAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNI 431
           QAVA+R+ ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+
Sbjct: 367 QAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNL 426

Query: 432 YPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYS 491
           YPR PM NQFNAITAQGRTDPNQNTG SI+NC I  ADDL  +S   VKT+LGRPWKEYS
Sbjct: 427 YPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYS 486

Query: 492 RTVYMQSFMDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTD 551
           RTV+MQS++D+++ P GWR W+GDFAL+T YYAE+ N G GS+T++RV WPG+H+IN TD
Sbjct: 487 RTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTD 546

Query: 552 AGNFTAGNFVLADDWLPQTGVPYTSGL 557
           A NFT  NF+L D W+ Q+GVPY SGL
Sbjct: 547 ANNFTVENFLLGDGWMVQSGVPYISGL 571

BLAST of PI0021431 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 655.6 bits (1690), Expect = 3.6e-188
Identity = 322/537 (59.96%), Postives = 411/537 (76.54%), Query Frame = 0

Query: 25  SASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLRSRSS 84
           +A+   ++C   PDPSYC+S LPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 85  LTVPA-VRALQDCQLLAGLNIDYLRTSFQAVNATSRALSEMKADDVQSLLSAILTNQQTC 144
           +   + V AL+DC+ LA L +DYL +S Q  ++T + LS  +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 145 LDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKK-KPTWKAAGRQG--- 204
           L+G+K+TA     +NGLS  L +DTKLY +SLA F+KGWVP++++ +P W+   R     
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 205 GFRNGRMSLKMSSRTQAIYERATRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTINEA 264
           GFRNG++ LKM+ R +A+Y   TRR LLQ+D    D + V DIV V Q+G+GNFTTIN A
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAA 270

Query: 265 IAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWT 324
           IAAAPN +  ++GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+TGNRSVVDGWT
Sbjct: 271 IAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWT 330

Query: 325 TFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHS 384
           TFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHS
Sbjct: 331 TFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHS 390

Query: 385 LRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIY 444
           LRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+I+
Sbjct: 391 LRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIH 450

Query: 445 NCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWDGDFALNTS 504
            C I  ADDLA  S   VKT+LGRPWKEYSRTV MQ+++D  + P+GW AW GDFAL+T 
Sbjct: 451 GCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 510

Query: 505 YYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 557
           YYAE+ N GPGS+T+ RV WPG+H+IN TDA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 511 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of PI0021431 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 487.3 bits (1253), Expect = 1.7e-137
Identity = 262/551 (47.55%), Postives = 354/551 (64.25%), Query Frame = 0

Query: 19  SLYITTSASPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLV 78
           SL  + S SP+   C STP P  C+   +A+ +   + Y YG+ + ++ L  + +  +++
Sbjct: 70  SLPPSQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 129

Query: 79  EKHLR----SRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRALSEMKADDVQS 138
             + R       S T   + A+ DC  L+ L+++YL T    +  T++ ++    + V S
Sbjct: 130 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNS 189

Query: 139 LLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKKPT 198
           LLS ++TNQQTCLDG+      ++    +  P+ + T+LYS+SL   +       K+   
Sbjct: 190 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKA 249

Query: 199 WKAAGRQGG---FRNGRMSLKMSSRTQAIYE---RATRRNLLQTDDGGDDQIAVRDIVVV 258
            K     GG   +R    +L    R     +   R T RNL +  +     I V   V+V
Sbjct: 250 SKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIV 309

Query: 259 SQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQ 318
               S NFTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+ +  NK+ LM++GDGIN+
Sbjct: 310 GPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINK 369

Query: 319 TVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYL 378
           T++TGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+FY 
Sbjct: 370 TIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYR 429

Query: 379 CSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQ 438
           CSFE YQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM+ Q NAITA 
Sbjct: 430 CSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAH 489

Query: 439 GRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPA 498
           GR DPNQNTG SI NC I AA DLA    + + TFLGRPWK YSRTV+MQS++ D++ P 
Sbjct: 490 GRLDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPV 549

Query: 499 GWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWL 557
           GW  W+G   L+T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  NF + D WL
Sbjct: 550 GWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWL 609

BLAST of PI0021431 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 487.3 bits (1253), Expect = 1.7e-137
Identity = 264/545 (48.44%), Postives = 348/545 (63.85%), Query Frame = 0

Query: 25  SASPTGSVCSSTPDPSYCK---SALPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKHLR- 84
           S  P    C STP P  C+   SA+ +   + Y YG+ + ++ L  +++  +++ +  + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 85  ---SRSSLTVPAVRALQDCQLLAGLNIDYLRTSFQAVNATSRALSEMKADDVQSLLSAIL 144
                 + TV  V A+ DC  LA L+++YL T  + + A +  ++    D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKA-AELMTAALVDRVTSLLGGVV 199

Query: 145 TNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVPKKKKKPTWKAAGR 204
           TNQQTCLDG+      ++    +  PL + T+LYS+SL   +       K+    K    
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIF 259

Query: 205 QGGFRNGRMSL----KMSSRT--QAIYERATRRNLLQTDDGGDDQIAVRDIVVVSQDGSG 264
            GG +  R  L    K+  +T  +    R   RNL +  +     I VR+ V V    + 
Sbjct: 260 GGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETD 319

Query: 265 NFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDGINQTVVTGN 324
           NF TI EA+AAAPN++ P  GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T+++GN
Sbjct: 320 NFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGN 379

Query: 325 RSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAY 384
            S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFE Y
Sbjct: 380 HSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGY 439

Query: 385 QDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPN 444
           QDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM+NQ NA+TA GRTDPN
Sbjct: 440 QDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPN 499

Query: 445 QNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLINPAGWRAWD 504
           Q TG SI NC I AA DLA +      TFLGRPWK YSRTVY+QS++ D++ P GW  W+
Sbjct: 500 QKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWN 559

Query: 505 GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVP 557
           G   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT +P
Sbjct: 560 GTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIP 619

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRX43.6e-20161.78Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q8RXK71.9e-19462.26Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
O222565.0e-18759.96Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Q94CB12.4e-13647.55Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF772.4e-13648.44Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5D3D3H40.0e+0097.86Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00132... [more]
A0A1S3BSI20.0e+0097.86Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1[more]
A0A0A0KB121.0e-30796.25Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1[more]
A0A6J1GQE61.7e-29191.01Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1[more]
A0A6J1JVJ52.8e-28990.29Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008451544.10.0e+0097.86PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KA... [more]
XP_004136013.12.1e-30796.25probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1... [more]
XP_038896831.12.3e-30695.19probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida][more]
KAG7014346.19.5e-29291.19putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
KAG6575804.11.2e-29191.19putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
AT1G02810.12.6e-20261.78Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.11.4e-19562.26Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.13.6e-18859.96Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.21.7e-13747.55Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.11.7e-13748.44Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 23..177
e-value: 6.0E-23
score: 92.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 31..176
e-value: 2.5E-17
score: 63.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 29..180
e-value: 3.9E-21
score: 77.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 31..180
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 237..556
e-value: 1.2E-118
score: 398.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 243..540
e-value: 6.6E-135
score: 448.6
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 14..556
NoneNo IPR availablePANTHERPTHR31707:SF294PECTINESTERASE/PECTINESTERASE INHIBITOR 7-RELATEDcoord: 14..556
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 31..179
e-value: 1.04471E-28
score: 109.45
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 389..398
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 238..555

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0021431.1PI0021431.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity