PI0021351 (gene) Melon (PI 482460) v1

Overview
NamePI0021351
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionKinetochore protein SPC25
Locationchr02: 24489771 .. 24492530 (+)
RNA-Seq ExpressionPI0021351
SyntenyPI0021351
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTCCGTCGATATTCTCCAGAAAAATAATTTAATTTTGAAGCTTGAAGCAAATTTGGCGGGCGAACGAGTGTACAGCAACTCCTTTGCTCTGTGAGAGAATGGAGAACAAGGCGACTGATTCTGTTCATGCAAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTGCAAATCAGTGGAATCGATCAATGTAAGGTCCCGAGCAAATGCCCTAAGCCAAGGTGAGCTATGTATTTTCTTCAATTTACTTGGGAAAGAAATGGGTGTGAAACTTGTTACTGTGGCCACTTACGACACTGTAATCGGTATTAGAATTGTTTGTGAAGTGACCATTAGGGTTTTTGAAATGGGTAGCGATTAGGACTCAGAACGGATGTAATTCTTGGGAATTGAGTTGGAGTTGGTTCAGAAGTGGCAAAGTTTACTTCGAAATTTCTTGTTTCATTTTTTTTTGTTTATTTGTTTATATATATTTTTCGAATTCTCAGGGAAACTGGGGGAACTAAAAGCGAGACTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGGTATGAGCTATGATTATGCTACTTTCCTTCGACGCTGTATGAGTTAGAATATACTACTTCGTTGAAGAGTGAATCGACTTGAAGTGGCATTTAAGAGTTGGGTTATTTTTCCACTTAAATTGTTGAGGATCTTTTCCAACGGAAGTGTTTTGGATTTGGAGTGAGTGATCGGCGAAATGTTGGTGTTTGTGATGCTGGTATATTGTTTCTTACTTAAAATTAGCGCTTGTCGTAAGGGCTTATTTCCTTTATACTTGCATATTTTTGTAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCAATTGCAACTTCAAAATCCAGAATAGAAGAGCTCAGGAATACCTTACAAGACTGCAAAGCAAGGAGGGATGAATATGCTACAATTATTTCTCAGCAATCCTTAGGTAATGTCTGTAAATCTGATTTATTTTCTTTCAGACCCAATTTCCCTTTATGCGAACAGAAAACTGCACATGGACTGAAGGTAAAGTCATTGTTAACAAGTTGGATATGCATTGTTCAATCTTTATTTGCTACTTACTTCCAAGATCTTTTCCACAATTCTTTTGCAAACCTCAACTTTAGAAGTTCCATGGGATAACTGATTACTTCTCTCTCTGTCCAATGCTATCAAAAGCTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAATGCAGAAATGAAATACAAGAGGCAATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGTATGATAATTCAAGTTTTTTTTTTTTTTTTGAGAATCTCTACAGCTTTTGGGAAGCTTGAGATAAATTTGTTGACAACTTCTCTCGGCATTTCAGGGGTGAAATTCTCTTTCAAGAAAATTAATATAGACAATCCTGATGAAGAATATTCTTTCACAATTCGTCATGCAAATGATACTTATATGTGTAAGTTTTATCATATGTGCACTTAATATACGTTGATTTTTCTTGCTCATTATCTTCTGACAACCTGTAACTTTTTCCTTAACTTTTTAGTGTTAGATTGTAACCCATCCTTGCCAGATATTAAGGAGTTGATACATGAATTAAATAAAACAAATGGTTTATTCAAGCTCGTCAGGATCATGAGACAGAGATTCCAGGAGGCTGCAGCAGAAGGTGTGCCCTGAGTTTTTAACTTTTTGCTTGAAAGGTATTTTTCCACTAAATGGTTGTTATATTTTTGTTATTCAACAGGAGCTGGGGCTCCAGCATTGTCTCTGCATCAAGGTTCAACGATAATCTCTATGTCAGCTCCAGGCTTGTCATCATCAAGTGAGAGAAGTGAATCTTCAACTGAAGAAATTAACAAGGATGAAGTTGAGGAAACTAACAAACATTCTAAGAACATTTTACCCAGAAAGAAACCGGCTATCCACTCCAGTGGATCTGCGTTTTCTTTGCGTCGATCTCCTCGTTTTAAGGTATGTTCAATTTTAATGTTTGATATCTGCTGCTTTTGATTTAGGATTCTCAGGTATATGATACTAAGAGAATACTATAAGTCTATAACTTCTATGTATAACAGGTTGATCTTTTTCTAAAGCACATTTCAAATTGTTATGCAAGAGAGAATTACTAATAAAATGCACCTTGTATGGCAGGTCAGGAAATAAGGTTGGAGGCTGTTAATGGTTGAACAATTATTAGGTTAATGGGCTTAGCCTTTTGCTTCCATGACATGAATTGCTTTGCAATTAACGTTGATTATGCCAATATGGGGTCCTGATCTCAACATTTGCCAGAACTTCTCTGACTATTCTGAAGAGTCCATATCATAAAAGCGAAGTTGAAGAAGACTTTATCACATGACCTGTTGGATGTTCGTTGTATCTGATTTTTTTTTATGTGTATTATTTTACACGAAGTCGTGGTTTTCATGTAATGCATGGGGTGATTATTCCAAATGTACTGGATAACAGTACTTTGTATCAATTTGATCTATATGCTAGGTTTCACATCATCGGACTTAAATTAATGTTTGTGCAGTTTTAAATTAATTAATTGCAAAAGCTTGAAGGTGAAAGTTCACTTCTCTATGAAATTAAGTTAGTATATAACGATGTAATTTACATTCTTTAAATAGTAGGTTCTCTTTCATTGTTTCGTTTTCAA

mRNA sequence

CGTCCGTCGATATTCTCCAGAAAAATAATTTAATTTTGAAGCTTGAAGCAAATTTGGCGGGCGAACGAGTGTACAGCAACTCCTTTGCTCTGTGAGAGAATGGAGAACAAGGCGACTGATTCTGTTCATGCAAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTGCAAATCAGTGGAATCGATCAATGTAAGGTCCCGAGCAAATGCCCTAAGCCAAGGGAAACTGGGGGAACTAAAAGCGAGACTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCAATTGCAACTTCAAAATCCAGAATAGAAGAGCTCAGGAATACCTTACAAGACTGCAAAGCAAGGAGGGATGAATATGCTACAATTATTTCTCAGCAATCCTTAGCTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAATGCAGAAATGAAATACAAGAGGCAATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGGGTGAAATTCTCTTTCAAGAAAATTAATATAGACAATCCTGATGAAGAATATTCTTTCACAATTCGTCATGCAAATGATACTTATATGTTGTTAGATTGTAACCCATCCTTGCCAGATATTAAGGAGTTGATACATGAATTAAATAAAACAAATGGTTTATTCAAGCTCGTCAGGATCATGAGACAGAGATTCCAGGAGGCTGCAGCAGAAGGAGCTGGGGCTCCAGCATTGTCTCTGCATCAAGGTTCAACGATAATCTCTATGTCAGCTCCAGGCTTGTCATCATCAAGTGAGAGAAGTGAATCTTCAACTGAAGAAATTAACAAGGATGAAGTTGAGGAAACTAACAAACATTCTAAGAACATTTTACCCAGAAAGAAACCGGCTATCCACTCCAGTGGATCTGCGTTTTCTTTGCGTCGATCTCCTCGTTTTAAGGTCAGGAAATAAGGTTGGAGGCTGTTAATGGTTGAACAATTATTAGGTTAATGGGCTTAGCCTTTTGCTTCCATGACATGAATTGCTTTGCAATTAACGTTGATTATGCCAATATGGGGTCCTGATCTCAACATTTGCCAGAACTTCTCTGACTATTCTGAAGAGTCCATATCATAAAAGCGAAGTTGAAGAAGACTTTATCACATGACCTGTTGGATGTTCGTTGTATCTGATTTTTTTTTATGTGTATTATTTTACACGAAGTCGTGGTTTTCATGTAATGCATGGGGTGATTATTCCAAATGTACTGGATAACAGTACTTTGTATCAATTTGATCTATATGCTAGGTTTCACATCATCGGACTTAAATTAATGTTTGTGCAGTTTTAAATTAATTAATTGCAAAAGCTTGAAGGTGAAAGTTCACTTCTCTATGAAATTAAGTTAGTATATAACGATGTAATTTACATTCTTTAAATAGTAGGTTCTCTTTCATTGTTTCGTTTTCAA

Coding sequence (CDS)

ATGGAGAACAAGGCGACTGATTCTGTTCATGCAAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTGCAAATCAGTGGAATCGATCAATGTAAGGTCCCGAGCAAATGCCCTAAGCCAAGGGAAACTGGGGGAACTAAAAGCGAGACTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCAATTGCAACTTCAAAATCCAGAATAGAAGAGCTCAGGAATACCTTACAAGACTGCAAAGCAAGGAGGGATGAATATGCTACAATTATTTCTCAGCAATCCTTAGCTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAATGCAGAAATGAAATACAAGAGGCAATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGGGTGAAATTCTCTTTCAAGAAAATTAATATAGACAATCCTGATGAAGAATATTCTTTCACAATTCGTCATGCAAATGATACTTATATGTTGTTAGATTGTAACCCATCCTTGCCAGATATTAAGGAGTTGATACATGAATTAAATAAAACAAATGGTTTATTCAAGCTCGTCAGGATCATGAGACAGAGATTCCAGGAGGCTGCAGCAGAAGGAGCTGGGGCTCCAGCATTGTCTCTGCATCAAGGTTCAACGATAATCTCTATGTCAGCTCCAGGCTTGTCATCATCAAGTGAGAGAAGTGAATCTTCAACTGAAGAAATTAACAAGGATGAAGTTGAGGAAACTAACAAACATTCTAAGAACATTTTACCCAGAAAGAAACCGGCTATCCACTCCAGTGGATCTGCGTTTTCTTTGCGTCGATCTCCTCGTTTTAAGGTCAGGAAATAA

Protein sequence

MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLGELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSPRFKVRK
Homology
BLAST of PI0021351 vs. ExPASy Swiss-Prot
Match: Q93VK9 (Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.3e-50
Identity = 132/310 (42.58%), Postives = 192/310 (61.94%), Query Frame = 0

Query: 7   DSVHARLEKLRIVRENEIPVQQQKMESF-ADSFCKSVESINVRSRANALSQGKLGELKAR 66
           D+    +  L ++ E +I  Q+ K++SF A  F +S+ S+  R++A A SQ +L  LKA 
Sbjct: 11  DTTKETMASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKAD 70

Query: 67  LREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQ 126
           LREAEDELVK LAVKTRKEA+++ I DSI+ ++SRIE LR  LQ  K+++D+   IISQQ
Sbjct: 71  LREAEDELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQ 130

Query: 127 SLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSF 186
             ALS S+    + +E + +I EAISWY   L FH+E G+GVKF+F  I+   P  E+SF
Sbjct: 131 LQALSKSKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSF 190

Query: 187 TIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSL 246
           T+ + ND Y LLD +  L  I E++ ELNKTN LF+ VR+MR++F ++        +  L
Sbjct: 191 TVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQL 250

Query: 247 HQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSL 306
            Q ++ IS SAP +S S++ + S+ E  NK    + N+  K      +  + +  S  + 
Sbjct: 251 QQETSAISASAPAISFSTDTNMSTPE--NKRSKVQVNRRQKR---GSESPLLAPVSTSAT 310

Query: 307 RRSPRFKVRK 316
           RRS RFK +K
Sbjct: 311 RRSSRFKGKK 315

BLAST of PI0021351 vs. ExPASy TrEMBL
Match: A0A1S3CM71 (Kinetochore protein SPC25 OS=Cucumis melo OX=3656 GN=LOC103502389 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.2e-157
Identity = 303/315 (96.19%), Postives = 309/315 (98.10%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
           ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQGKLG
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLR AEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRVAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSL LSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240

Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSG 300
           PALSLHQGS+IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSG
Sbjct: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

BLAST of PI0021351 vs. ExPASy TrEMBL
Match: A0A0A0LU84 (Kinetochore protein SPC25 OS=Cucumis sativus OX=3659 GN=Csa_1G004230 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.1e-153
Identity = 297/315 (94.29%), Postives = 303/315 (96.19%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
           MENKA DSV ARLEKLRI+RENEIPVQQQKMESFADSF KSVESI+VRSRANALSQGKLG
Sbjct: 1   MENKANDSVRARLEKLRILRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLRE EDELVKALAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSL LSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMR+RFQEAAAEGAGA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240

Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSG 300
           PALSLHQGS++IS SAP LSSS+E SESSTEEIN DEVEETNKHSKNILPRK PAIHSSG
Sbjct: 241 PALSLHQGSSVISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

BLAST of PI0021351 vs. ExPASy TrEMBL
Match: A0A5D3BFE9 (Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001250 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 5.4e-149
Identity = 301/364 (82.69%), Postives = 307/364 (84.34%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQG--- 60
           ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLREA 120
                                                           KLGELKARLR A
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLAL 180
           EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSL L
Sbjct: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGS 300
           ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 TIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
           +IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

BLAST of PI0021351 vs. ExPASy TrEMBL
Match: A0A5A7UUK0 (Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001230 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.6e-148
Identity = 300/364 (82.42%), Postives = 306/364 (84.07%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQG--- 60
           ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLREA 120
                                                           KLGELKARLR A
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLAL 180
           EDELVK LAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSL L
Sbjct: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGS 300
           ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 TIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
           +IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

BLAST of PI0021351 vs. ExPASy TrEMBL
Match: A0A6J1BYG5 (Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.6e-132
Identity = 262/316 (82.91%), Postives = 285/316 (90.19%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
           MENK  DS+  ++E LR+VRE EIPVQQQKMESFADSF KS+ESI VRSR NALSQGKLG
Sbjct: 1   MENKVEDSIRVKMESLRMVREKEIPVQQQKMESFADSFRKSLESITVRSRENALSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLREAEDELVKALAVKTRKEAKRL I DS+A SKSRIEELRNTLQDCKA+RDEY+T
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLAITDSLAASKSRIEELRNTLQDCKAKRDEYST 120

Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSLALSSSE KETQES+CR+EIQEAISWY RVLDFHIEGG GVKFSFKKINI+ PD
Sbjct: 121 IISQQSLALSSSEHKETQESDCRSEIQEAISWYNRVLDFHIEGGRGVKFSFKKININIPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
           +EYSFTIRHA+DTY LLDCNPSL DI++LIHELNKTNGLFK VR+MRQRFQE AAEG GA
Sbjct: 181 KEYSFTIRHASDTYTLLDCNPSLQDIQDLIHELNKTNGLFKFVRVMRQRFQEIAAEGVGA 240

Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEI-NKDEVEETNKHSKNILPRKKPAIHSS 300
            ALSLHQ STIIS+SAPGLSSS++RSESST+EI N+  +EETNKHSK IL  KKPA+ S 
Sbjct: 241 QALSLHQDSTIISVSAPGLSSSTDRSESSTQEIDNQVRLEETNKHSKKILSGKKPAVLSP 300

Query: 301 GSAFSLRRSPRFKVRK 316
           GSAFSLRRSPRFKVRK
Sbjct: 301 GSAFSLRRSPRFKVRK 316

BLAST of PI0021351 vs. NCBI nr
Match: XP_008464538.1 (PREDICTED: probable kinetochore protein SPC25 [Cucumis melo])

HSP 1 Score: 564.3 bits (1453), Expect = 6.6e-157
Identity = 303/315 (96.19%), Postives = 309/315 (98.10%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
           ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQGKLG
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLR AEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRVAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSL LSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240

Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSG 300
           PALSLHQGS+IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSG
Sbjct: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

BLAST of PI0021351 vs. NCBI nr
Match: XP_004138099.1 (kinetochore protein SPC25 homolog [Cucumis sativus] >KGN63556.1 hypothetical protein Csa_014306 [Cucumis sativus])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 297/315 (94.29%), Postives = 303/315 (96.19%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
           MENKA DSV ARLEKLRI+RENEIPVQQQKMESFADSF KSVESI+VRSRANALSQGKLG
Sbjct: 1   MENKANDSVRARLEKLRILRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLRE EDELVKALAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYAT
Sbjct: 61  ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120

Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IISQQSL LSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMR+RFQEAAAEGAGA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240

Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSG 300
           PALSLHQGS++IS SAP LSSS+E SESSTEEIN DEVEETNKHSKNILPRK PAIHSSG
Sbjct: 241 PALSLHQGSSVISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300

Query: 301 SAFSLRRSPRFKVRK 316
           SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315

BLAST of PI0021351 vs. NCBI nr
Match: TYJ98520.1 (putative kinetochore protein SPC25 [Cucumis melo var. makuwa])

HSP 1 Score: 537.0 bits (1382), Expect = 1.1e-148
Identity = 301/364 (82.69%), Postives = 307/364 (84.34%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQG--- 60
           ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLREA 120
                                                           KLGELKARLR A
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLAL 180
           EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSL L
Sbjct: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGS 300
           ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 TIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
           +IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

BLAST of PI0021351 vs. NCBI nr
Match: KAA0057836.1 (putative kinetochore protein SPC25 [Cucumis melo var. makuwa])

HSP 1 Score: 535.4 bits (1378), Expect = 3.3e-148
Identity = 300/364 (82.42%), Postives = 306/364 (84.07%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQG--- 60
           ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQG   
Sbjct: 1   MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60

Query: 61  ------------------------------------------------KLGELKARLREA 120
                                                           KLGELKARLR A
Sbjct: 61  IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120

Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLAL 180
           EDELVK LAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSL L
Sbjct: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180

Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
           SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240

Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGS 300
           ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300

Query: 301 TIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
           +IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360

BLAST of PI0021351 vs. NCBI nr
Match: XP_038880541.1 (kinetochore protein SPC25 homolog [Benincasa hispida])

HSP 1 Score: 520.0 bits (1338), Expect = 1.4e-143
Identity = 285/316 (90.19%), Postives = 297/316 (93.99%), Query Frame = 0

Query: 1   MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
           MENKA DSV ARLEKLRIVRE EIP QQQKMESF +SF KSVESINVRSRANALSQGKLG
Sbjct: 1   MENKAKDSVRARLEKLRIVREKEIPFQQQKMESFVNSFYKSVESINVRSRANALSQGKLG 60

Query: 61  ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
           ELKARLREAEDELVKALAVKTRKEAKRLTIIDS+ATSKSRIEELRNTLQD KARRDEY+T
Sbjct: 61  ELKARLREAEDELVKALAVKTRKEAKRLTIIDSLATSKSRIEELRNTLQDSKARRDEYST 120

Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
           IIS+QSLALSSSERKE QESECR EIQEAISWYKRVLDFHIEGG+GVKFSFKKIN+ NPD
Sbjct: 121 IISRQSLALSSSERKEPQESECRKEIQEAISWYKRVLDFHIEGGHGVKFSFKKINVVNPD 180

Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
           EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAA+G  A
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAKGVEA 240

Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEIN-KDEVEETNKHSKNILPRKKPAIHSS 300
            ALSLHQGS II MSAPGLSSS+ERSESS+EEIN KDE+EETNKH+K ILP KKPAI SS
Sbjct: 241 QALSLHQGSMIIPMSAPGLSSSTERSESSSEEINKKDELEETNKHTKKILPGKKPAIKSS 300

Query: 301 GSAFSLRRSPRFKVRK 316
           GSAFSLRRSPRFKVRK
Sbjct: 301 GSAFSLRRSPRFKVRK 316

BLAST of PI0021351 vs. TAIR 10
Match: AT3G48210.1 (CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:IPR013255); Has 194 Blast hits to 194 proteins in 72 species: Archae - 0; Bacteria - 4; Metazoa - 72; Fungi - 39; Plants - 62; Viruses - 0; Other Eukaryotes - 17 (source: NCBI BLink). )

HSP 1 Score: 200.3 bits (508), Expect = 2.3e-51
Identity = 132/310 (42.58%), Postives = 192/310 (61.94%), Query Frame = 0

Query: 7   DSVHARLEKLRIVRENEIPVQQQKMESF-ADSFCKSVESINVRSRANALSQGKLGELKAR 66
           D+    +  L ++ E +I  Q+ K++SF A  F +S+ S+  R++A A SQ +L  LKA 
Sbjct: 11  DTTKETMASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKAD 70

Query: 67  LREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQ 126
           LREAEDELVK LAVKTRKEA+++ I DSI+ ++SRIE LR  LQ  K+++D+   IISQQ
Sbjct: 71  LREAEDELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQ 130

Query: 127 SLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSF 186
             ALS S+    + +E + +I EAISWY   L FH+E G+GVKF+F  I+   P  E+SF
Sbjct: 131 LQALSKSKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSF 190

Query: 187 TIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSL 246
           T+ + ND Y LLD +  L  I E++ ELNKTN LF+ VR+MR++F ++        +  L
Sbjct: 191 TVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQL 250

Query: 247 HQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSL 306
            Q ++ IS SAP +S S++ + S+ E  NK    + N+  K      +  + +  S  + 
Sbjct: 251 QQETSAISASAPAISFSTDTNMSTPE--NKRSKVQVNRRQKR---GSESPLLAPVSTSAT 310

Query: 307 RRSPRFKVRK 316
           RRS RFK +K
Sbjct: 311 RRSSRFKGKK 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VK93.3e-5042.58Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CM713.2e-15796.19Kinetochore protein SPC25 OS=Cucumis melo OX=3656 GN=LOC103502389 PE=3 SV=1[more]
A0A0A0LU848.1e-15394.29Kinetochore protein SPC25 OS=Cucumis sativus OX=3659 GN=Csa_1G004230 PE=3 SV=1[more]
A0A5D3BFE95.4e-14982.69Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7UUK01.6e-14882.42Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A6J1BYG51.6e-13282.91Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_008464538.16.6e-15796.19PREDICTED: probable kinetochore protein SPC25 [Cucumis melo][more]
XP_004138099.11.7e-15294.29kinetochore protein SPC25 homolog [Cucumis sativus] >KGN63556.1 hypothetical pro... [more]
TYJ98520.11.1e-14882.69putative kinetochore protein SPC25 [Cucumis melo var. makuwa][more]
KAA0057836.13.3e-14882.42putative kinetochore protein SPC25 [Cucumis melo var. makuwa][more]
XP_038880541.11.4e-14390.19kinetochore protein SPC25 homolog [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G48210.12.3e-5142.58CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:I... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 52..86
NoneNo IPR availableCOILSCoilCoilcoord: 94..121
NoneNo IPR availableGENE3D3.30.457.50Chromosome segregation protein Spc25coord: 138..237
e-value: 5.4E-25
score: 89.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..287
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 254..315
NoneNo IPR availablePANTHERPTHR14281:SF3SUBFAMILY NOT NAMEDcoord: 1..313
IPR013255Chromosome segregation protein Spc25, C-terminalPFAMPF08234Spindle_Spc25coord: 166..231
e-value: 1.4E-20
score: 73.3
IPR045143Kinetochore protein Spc25PANTHERPTHR14281KINETOCHORE PROTEIN SPC25-RELATEDcoord: 1..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0021351.1PI0021351.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007049 cell cycle
biological_process GO:0051301 cell division
biological_process GO:0007059 chromosome segregation
cellular_component GO:0031262 Ndc80 complex
cellular_component GO:0005634 nucleus