Sequences
The following sequences are available for this feature:
Gene sequence (with intron)
Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTCCGTCGATATTCTCCAGAAAAATAATTTAATTTTGAAGCTTGAAGCAAATTTGGCGGGCGAACGAGTGTACAGCAACTCCTTTGCTCTGTGAGAGAATGGAGAACAAGGCGACTGATTCTGTTCATGCAAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTGCAAATCAGTGGAATCGATCAATGTAAGGTCCCGAGCAAATGCCCTAAGCCAAGGTGAGCTATGTATTTTCTTCAATTTACTTGGGAAAGAAATGGGTGTGAAACTTGTTACTGTGGCCACTTACGACACTGTAATCGGTATTAGAATTGTTTGTGAAGTGACCATTAGGGTTTTTGAAATGGGTAGCGATTAGGACTCAGAACGGATGTAATTCTTGGGAATTGAGTTGGAGTTGGTTCAGAAGTGGCAAAGTTTACTTCGAAATTTCTTGTTTCATTTTTTTTTGTTTATTTGTTTATATATATTTTTCGAATTCTCAGGGAAACTGGGGGAACTAAAAGCGAGACTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGGTATGAGCTATGATTATGCTACTTTCCTTCGACGCTGTATGAGTTAGAATATACTACTTCGTTGAAGAGTGAATCGACTTGAAGTGGCATTTAAGAGTTGGGTTATTTTTCCACTTAAATTGTTGAGGATCTTTTCCAACGGAAGTGTTTTGGATTTGGAGTGAGTGATCGGCGAAATGTTGGTGTTTGTGATGCTGGTATATTGTTTCTTACTTAAAATTAGCGCTTGTCGTAAGGGCTTATTTCCTTTATACTTGCATATTTTTGTAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCAATTGCAACTTCAAAATCCAGAATAGAAGAGCTCAGGAATACCTTACAAGACTGCAAAGCAAGGAGGGATGAATATGCTACAATTATTTCTCAGCAATCCTTAGGTAATGTCTGTAAATCTGATTTATTTTCTTTCAGACCCAATTTCCCTTTATGCGAACAGAAAACTGCACATGGACTGAAGGTAAAGTCATTGTTAACAAGTTGGATATGCATTGTTCAATCTTTATTTGCTACTTACTTCCAAGATCTTTTCCACAATTCTTTTGCAAACCTCAACTTTAGAAGTTCCATGGGATAACTGATTACTTCTCTCTCTGTCCAATGCTATCAAAAGCTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAATGCAGAAATGAAATACAAGAGGCAATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGTATGATAATTCAAGTTTTTTTTTTTTTTTTGAGAATCTCTACAGCTTTTGGGAAGCTTGAGATAAATTTGTTGACAACTTCTCTCGGCATTTCAGGGGTGAAATTCTCTTTCAAGAAAATTAATATAGACAATCCTGATGAAGAATATTCTTTCACAATTCGTCATGCAAATGATACTTATATGTGTAAGTTTTATCATATGTGCACTTAATATACGTTGATTTTTCTTGCTCATTATCTTCTGACAACCTGTAACTTTTTCCTTAACTTTTTAGTGTTAGATTGTAACCCATCCTTGCCAGATATTAAGGAGTTGATACATGAATTAAATAAAACAAATGGTTTATTCAAGCTCGTCAGGATCATGAGACAGAGATTCCAGGAGGCTGCAGCAGAAGGTGTGCCCTGAGTTTTTAACTTTTTGCTTGAAAGGTATTTTTCCACTAAATGGTTGTTATATTTTTGTTATTCAACAGGAGCTGGGGCTCCAGCATTGTCTCTGCATCAAGGTTCAACGATAATCTCTATGTCAGCTCCAGGCTTGTCATCATCAAGTGAGAGAAGTGAATCTTCAACTGAAGAAATTAACAAGGATGAAGTTGAGGAAACTAACAAACATTCTAAGAACATTTTACCCAGAAAGAAACCGGCTATCCACTCCAGTGGATCTGCGTTTTCTTTGCGTCGATCTCCTCGTTTTAAGGTATGTTCAATTTTAATGTTTGATATCTGCTGCTTTTGATTTAGGATTCTCAGGTATATGATACTAAGAGAATACTATAAGTCTATAACTTCTATGTATAACAGGTTGATCTTTTTCTAAAGCACATTTCAAATTGTTATGCAAGAGAGAATTACTAATAAAATGCACCTTGTATGGCAGGTCAGGAAATAAGGTTGGAGGCTGTTAATGGTTGAACAATTATTAGGTTAATGGGCTTAGCCTTTTGCTTCCATGACATGAATTGCTTTGCAATTAACGTTGATTATGCCAATATGGGGTCCTGATCTCAACATTTGCCAGAACTTCTCTGACTATTCTGAAGAGTCCATATCATAAAAGCGAAGTTGAAGAAGACTTTATCACATGACCTGTTGGATGTTCGTTGTATCTGATTTTTTTTTATGTGTATTATTTTACACGAAGTCGTGGTTTTCATGTAATGCATGGGGTGATTATTCCAAATGTACTGGATAACAGTACTTTGTATCAATTTGATCTATATGCTAGGTTTCACATCATCGGACTTAAATTAATGTTTGTGCAGTTTTAAATTAATTAATTGCAAAAGCTTGAAGGTGAAAGTTCACTTCTCTATGAAATTAAGTTAGTATATAACGATGTAATTTACATTCTTTAAATAGTAGGTTCTCTTTCATTGTTTCGTTTTCAA
mRNA sequence
CGTCCGTCGATATTCTCCAGAAAAATAATTTAATTTTGAAGCTTGAAGCAAATTTGGCGGGCGAACGAGTGTACAGCAACTCCTTTGCTCTGTGAGAGAATGGAGAACAAGGCGACTGATTCTGTTCATGCAAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTGCAAATCAGTGGAATCGATCAATGTAAGGTCCCGAGCAAATGCCCTAAGCCAAGGGAAACTGGGGGAACTAAAAGCGAGACTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCAATTGCAACTTCAAAATCCAGAATAGAAGAGCTCAGGAATACCTTACAAGACTGCAAAGCAAGGAGGGATGAATATGCTACAATTATTTCTCAGCAATCCTTAGCTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAATGCAGAAATGAAATACAAGAGGCAATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGGGTGAAATTCTCTTTCAAGAAAATTAATATAGACAATCCTGATGAAGAATATTCTTTCACAATTCGTCATGCAAATGATACTTATATGTTGTTAGATTGTAACCCATCCTTGCCAGATATTAAGGAGTTGATACATGAATTAAATAAAACAAATGGTTTATTCAAGCTCGTCAGGATCATGAGACAGAGATTCCAGGAGGCTGCAGCAGAAGGAGCTGGGGCTCCAGCATTGTCTCTGCATCAAGGTTCAACGATAATCTCTATGTCAGCTCCAGGCTTGTCATCATCAAGTGAGAGAAGTGAATCTTCAACTGAAGAAATTAACAAGGATGAAGTTGAGGAAACTAACAAACATTCTAAGAACATTTTACCCAGAAAGAAACCGGCTATCCACTCCAGTGGATCTGCGTTTTCTTTGCGTCGATCTCCTCGTTTTAAGGTCAGGAAATAAGGTTGGAGGCTGTTAATGGTTGAACAATTATTAGGTTAATGGGCTTAGCCTTTTGCTTCCATGACATGAATTGCTTTGCAATTAACGTTGATTATGCCAATATGGGGTCCTGATCTCAACATTTGCCAGAACTTCTCTGACTATTCTGAAGAGTCCATATCATAAAAGCGAAGTTGAAGAAGACTTTATCACATGACCTGTTGGATGTTCGTTGTATCTGATTTTTTTTTATGTGTATTATTTTACACGAAGTCGTGGTTTTCATGTAATGCATGGGGTGATTATTCCAAATGTACTGGATAACAGTACTTTGTATCAATTTGATCTATATGCTAGGTTTCACATCATCGGACTTAAATTAATGTTTGTGCAGTTTTAAATTAATTAATTGCAAAAGCTTGAAGGTGAAAGTTCACTTCTCTATGAAATTAAGTTAGTATATAACGATGTAATTTACATTCTTTAAATAGTAGGTTCTCTTTCATTGTTTCGTTTTCAA
Coding sequence (CDS)
ATGGAGAACAAGGCGACTGATTCTGTTCATGCAAGGTTGGAAAAACTTCGAATCGTTCGTGAAAATGAAATTCCAGTTCAGCAGCAAAAGATGGAGTCCTTCGCGGATTCATTTTGCAAATCAGTGGAATCGATCAATGTAAGGTCCCGAGCAAATGCCCTAAGCCAAGGGAAACTGGGGGAACTAAAAGCGAGACTGAGGGAGGCGGAAGATGAACTGGTTAAAGCTCTTGCAGTGAAGACTCGTAAAGAGGCCAAACGCCTCACAATCATCGACTCAATTGCAACTTCAAAATCCAGAATAGAAGAGCTCAGGAATACCTTACAAGACTGCAAAGCAAGGAGGGATGAATATGCTACAATTATTTCTCAGCAATCCTTAGCTTTATCTTCATCTGAACGCAAGGAAACCCAGGAGAGCGAATGCAGAAATGAAATACAAGAGGCAATTTCTTGGTACAAAAGGGTTCTTGATTTTCATATTGAAGGAGGATATGGGGTGAAATTCTCTTTCAAGAAAATTAATATAGACAATCCTGATGAAGAATATTCTTTCACAATTCGTCATGCAAATGATACTTATATGTTGTTAGATTGTAACCCATCCTTGCCAGATATTAAGGAGTTGATACATGAATTAAATAAAACAAATGGTTTATTCAAGCTCGTCAGGATCATGAGACAGAGATTCCAGGAGGCTGCAGCAGAAGGAGCTGGGGCTCCAGCATTGTCTCTGCATCAAGGTTCAACGATAATCTCTATGTCAGCTCCAGGCTTGTCATCATCAAGTGAGAGAAGTGAATCTTCAACTGAAGAAATTAACAAGGATGAAGTTGAGGAAACTAACAAACATTCTAAGAACATTTTACCCAGAAAGAAACCGGCTATCCACTCCAGTGGATCTGCGTTTTCTTTGCGTCGATCTCCTCGTTTTAAGGTCAGGAAATAA
Protein sequence
MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLGELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSPRFKVRK
Homology
BLAST of PI0021351 vs. ExPASy Swiss-Prot
Match:
Q93VK9 (Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 SV=1)
HSP 1 Score: 200.3 bits (508), Expect = 3.3e-50
Identity = 132/310 (42.58%), Postives = 192/310 (61.94%), Query Frame = 0
Query: 7 DSVHARLEKLRIVRENEIPVQQQKMESF-ADSFCKSVESINVRSRANALSQGKLGELKAR 66
D+ + L ++ E +I Q+ K++SF A F +S+ S+ R++A A SQ +L LKA
Sbjct: 11 DTTKETMASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKAD 70
Query: 67 LREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQ 126
LREAEDELVK LAVKTRKEA+++ I DSI+ ++SRIE LR LQ K+++D+ IISQQ
Sbjct: 71 LREAEDELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQ 130
Query: 127 SLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSF 186
ALS S+ + +E + +I EAISWY L FH+E G+GVKF+F I+ P E+SF
Sbjct: 131 LQALSKSKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSF 190
Query: 187 TIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSL 246
T+ + ND Y LLD + L I E++ ELNKTN LF+ VR+MR++F ++ + L
Sbjct: 191 TVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQL 250
Query: 247 HQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSL 306
Q ++ IS SAP +S S++ + S+ E NK + N+ K + + + S +
Sbjct: 251 QQETSAISASAPAISFSTDTNMSTPE--NKRSKVQVNRRQKR---GSESPLLAPVSTSAT 310
Query: 307 RRSPRFKVRK 316
RRS RFK +K
Sbjct: 311 RRSSRFKGKK 315
BLAST of PI0021351 vs. ExPASy TrEMBL
Match:
A0A1S3CM71 (Kinetochore protein SPC25 OS=Cucumis melo OX=3656 GN=LOC103502389 PE=3 SV=1)
HSP 1 Score: 564.3 bits (1453), Expect = 3.2e-157
Identity = 303/315 (96.19%), Postives = 309/315 (98.10%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQGKLG
Sbjct: 1 MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60
Query: 61 ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
ELKARLR AEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT
Sbjct: 61 ELKARLRVAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
IISQQSL LSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240
Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSG 300
PALSLHQGS+IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSG
Sbjct: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300
Query: 301 SAFSLRRSPRFKVRK 316
SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315
BLAST of PI0021351 vs. ExPASy TrEMBL
Match:
A0A0A0LU84 (Kinetochore protein SPC25 OS=Cucumis sativus OX=3659 GN=Csa_1G004230 PE=3 SV=1)
HSP 1 Score: 549.7 bits (1415), Expect = 8.1e-153
Identity = 297/315 (94.29%), Postives = 303/315 (96.19%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
MENKA DSV ARLEKLRI+RENEIPVQQQKMESFADSF KSVESI+VRSRANALSQGKLG
Sbjct: 1 MENKANDSVRARLEKLRILRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60
Query: 61 ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
ELKARLRE EDELVKALAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYAT
Sbjct: 61 ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120
Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
IISQQSL LSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
EEYSFTIRHANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMR+RFQEAAAEGAGA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240
Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSG 300
PALSLHQGS++IS SAP LSSS+E SESSTEEIN DEVEETNKHSKNILPRK PAIHSSG
Sbjct: 241 PALSLHQGSSVISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300
Query: 301 SAFSLRRSPRFKVRK 316
SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315
BLAST of PI0021351 vs. ExPASy TrEMBL
Match:
A0A5D3BFE9 (Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001250 PE=3 SV=1)
HSP 1 Score: 537.0 bits (1382), Expect = 5.4e-149
Identity = 301/364 (82.69%), Postives = 307/364 (84.34%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQG--- 60
ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQG
Sbjct: 1 MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60
Query: 61 ------------------------------------------------KLGELKARLREA 120
KLGELKARLR A
Sbjct: 61 IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120
Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLAL 180
EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSL L
Sbjct: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180
Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGS 300
ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300
Query: 301 TIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
+IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360
BLAST of PI0021351 vs. ExPASy TrEMBL
Match:
A0A5A7UUK0 (Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001230 PE=3 SV=1)
HSP 1 Score: 535.4 bits (1378), Expect = 1.6e-148
Identity = 300/364 (82.42%), Postives = 306/364 (84.07%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQG--- 60
ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQG
Sbjct: 1 MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60
Query: 61 ------------------------------------------------KLGELKARLREA 120
KLGELKARLR A
Sbjct: 61 IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120
Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLAL 180
EDELVK LAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSL L
Sbjct: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180
Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGS 300
ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300
Query: 301 TIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
+IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360
BLAST of PI0021351 vs. ExPASy TrEMBL
Match:
A0A6J1BYG5 (Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV=1)
HSP 1 Score: 482.3 bits (1240), Expect = 1.6e-132
Identity = 262/316 (82.91%), Postives = 285/316 (90.19%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
MENK DS+ ++E LR+VRE EIPVQQQKMESFADSF KS+ESI VRSR NALSQGKLG
Sbjct: 1 MENKVEDSIRVKMESLRMVREKEIPVQQQKMESFADSFRKSLESITVRSRENALSQGKLG 60
Query: 61 ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
ELKARLREAEDELVKALAVKTRKEAKRL I DS+A SKSRIEELRNTLQDCKA+RDEY+T
Sbjct: 61 ELKARLREAEDELVKALAVKTRKEAKRLAITDSLAASKSRIEELRNTLQDCKAKRDEYST 120
Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
IISQQSLALSSSE KETQES+CR+EIQEAISWY RVLDFHIEGG GVKFSFKKINI+ PD
Sbjct: 121 IISQQSLALSSSEHKETQESDCRSEIQEAISWYNRVLDFHIEGGRGVKFSFKKININIPD 180
Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
+EYSFTIRHA+DTY LLDCNPSL DI++LIHELNKTNGLFK VR+MRQRFQE AAEG GA
Sbjct: 181 KEYSFTIRHASDTYTLLDCNPSLQDIQDLIHELNKTNGLFKFVRVMRQRFQEIAAEGVGA 240
Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEI-NKDEVEETNKHSKNILPRKKPAIHSS 300
ALSLHQ STIIS+SAPGLSSS++RSESST+EI N+ +EETNKHSK IL KKPA+ S
Sbjct: 241 QALSLHQDSTIISVSAPGLSSSTDRSESSTQEIDNQVRLEETNKHSKKILSGKKPAVLSP 300
Query: 301 GSAFSLRRSPRFKVRK 316
GSAFSLRRSPRFKVRK
Sbjct: 301 GSAFSLRRSPRFKVRK 316
BLAST of PI0021351 vs. NCBI nr
Match:
XP_008464538.1 (PREDICTED: probable kinetochore protein SPC25 [Cucumis melo])
HSP 1 Score: 564.3 bits (1453), Expect = 6.6e-157
Identity = 303/315 (96.19%), Postives = 309/315 (98.10%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQGKLG
Sbjct: 1 MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGKLG 60
Query: 61 ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
ELKARLR AEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT
Sbjct: 61 ELKARLRVAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
IISQQSL LSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGA 240
Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSG 300
PALSLHQGS+IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSG
Sbjct: 241 PALSLHQGSSIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSG 300
Query: 301 SAFSLRRSPRFKVRK 316
SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315
BLAST of PI0021351 vs. NCBI nr
Match:
XP_004138099.1 (kinetochore protein SPC25 homolog [Cucumis sativus] >KGN63556.1 hypothetical protein Csa_014306 [Cucumis sativus])
HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 297/315 (94.29%), Postives = 303/315 (96.19%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
MENKA DSV ARLEKLRI+RENEIPVQQQKMESFADSF KSVESI+VRSRANALSQGKLG
Sbjct: 1 MENKANDSVRARLEKLRILRENEIPVQQQKMESFADSFYKSVESISVRSRANALSQGKLG 60
Query: 61 ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
ELKARLRE EDELVKALAVKTRKEAKRLTIIDSIATSKSR EELRNTLQDCKARRDEYAT
Sbjct: 61 ELKARLRETEDELVKALAVKTRKEAKRLTIIDSIATSKSRTEELRNTLQDCKARRDEYAT 120
Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
IISQQSL LSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD
Sbjct: 121 IISQQSLVLSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
EEYSFTIRHANDTYMLLDCNPSL DIKELIHELNKTNGLFKLVRIMR+RFQEAAAEGAGA
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLSDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGAGA 240
Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSG 300
PALSLHQGS++IS SAP LSSS+E SESSTEEIN DEVEETNKHSKNILPRK PAIHSSG
Sbjct: 241 PALSLHQGSSVISTSAPVLSSSTEGSESSTEEINIDEVEETNKHSKNILPRKNPAIHSSG 300
Query: 301 SAFSLRRSPRFKVRK 316
SAFSLRRSPRFKVRK
Sbjct: 301 SAFSLRRSPRFKVRK 315
BLAST of PI0021351 vs. NCBI nr
Match:
TYJ98520.1 (putative kinetochore protein SPC25 [Cucumis melo var. makuwa])
HSP 1 Score: 537.0 bits (1382), Expect = 1.1e-148
Identity = 301/364 (82.69%), Postives = 307/364 (84.34%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQG--- 60
ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQG
Sbjct: 1 MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60
Query: 61 ------------------------------------------------KLGELKARLREA 120
KLGELKARLR A
Sbjct: 61 IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120
Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLAL 180
EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSL L
Sbjct: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180
Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGS 300
ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300
Query: 301 TIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
+IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360
BLAST of PI0021351 vs. NCBI nr
Match:
KAA0057836.1 (putative kinetochore protein SPC25 [Cucumis melo var. makuwa])
HSP 1 Score: 535.4 bits (1378), Expect = 3.3e-148
Identity = 300/364 (82.42%), Postives = 306/364 (84.07%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQG--- 60
ME KATDSV ARLEKLRIVRENEIPVQQQKMESFADSFCKSVESI+VRSRANALSQG
Sbjct: 1 MEIKATDSVRARLEKLRIVRENEIPVQQQKMESFADSFCKSVESISVRSRANALSQGELS 60
Query: 61 ------------------------------------------------KLGELKARLREA 120
KLGELKARLR A
Sbjct: 61 IFFILFGKEMGVKLVTVATYDTVIDIRMVCEVTIRVLKWVAIKTQNGWKLGELKARLRVA 120
Query: 121 EDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLAL 180
EDELVK LAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSL L
Sbjct: 121 EDELVKTLAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQSLVL 180
Query: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH
Sbjct: 181 SSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSFTIRH 240
Query: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSLHQGS 300
ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAAEG+GAPALSLHQGS
Sbjct: 241 ANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAEGSGAPALSLHQGS 300
Query: 301 TIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 314
+IIS SAPGLSSS+ERS+SSTEEIN DEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP
Sbjct: 301 SIISTSAPGLSSSTERSDSSTEEINMDEVEETNKHSKNILPRKKPAIHSSGSAFSLRRSP 360
BLAST of PI0021351 vs. NCBI nr
Match:
XP_038880541.1 (kinetochore protein SPC25 homolog [Benincasa hispida])
HSP 1 Score: 520.0 bits (1338), Expect = 1.4e-143
Identity = 285/316 (90.19%), Postives = 297/316 (93.99%), Query Frame = 0
Query: 1 MENKATDSVHARLEKLRIVRENEIPVQQQKMESFADSFCKSVESINVRSRANALSQGKLG 60
MENKA DSV ARLEKLRIVRE EIP QQQKMESF +SF KSVESINVRSRANALSQGKLG
Sbjct: 1 MENKAKDSVRARLEKLRIVREKEIPFQQQKMESFVNSFYKSVESINVRSRANALSQGKLG 60
Query: 61 ELKARLREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYAT 120
ELKARLREAEDELVKALAVKTRKEAKRLTIIDS+ATSKSRIEELRNTLQD KARRDEY+T
Sbjct: 61 ELKARLREAEDELVKALAVKTRKEAKRLTIIDSLATSKSRIEELRNTLQDSKARRDEYST 120
Query: 121 IISQQSLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPD 180
IIS+QSLALSSSERKE QESECR EIQEAISWYKRVLDFHIEGG+GVKFSFKKIN+ NPD
Sbjct: 121 IISRQSLALSSSERKEPQESECRKEIQEAISWYKRVLDFHIEGGHGVKFSFKKINVVNPD 180
Query: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGA 240
EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMR+RFQEAAA+G A
Sbjct: 181 EEYSFTIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRRRFQEAAAKGVEA 240
Query: 241 PALSLHQGSTIISMSAPGLSSSSERSESSTEEIN-KDEVEETNKHSKNILPRKKPAIHSS 300
ALSLHQGS II MSAPGLSSS+ERSESS+EEIN KDE+EETNKH+K ILP KKPAI SS
Sbjct: 241 QALSLHQGSMIIPMSAPGLSSSTERSESSSEEINKKDELEETNKHTKKILPGKKPAIKSS 300
Query: 301 GSAFSLRRSPRFKVRK 316
GSAFSLRRSPRFKVRK
Sbjct: 301 GSAFSLRRSPRFKVRK 316
BLAST of PI0021351 vs. TAIR 10
Match:
AT3G48210.1 (CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:IPR013255); Has 194 Blast hits to 194 proteins in 72 species: Archae - 0; Bacteria - 4; Metazoa - 72; Fungi - 39; Plants - 62; Viruses - 0; Other Eukaryotes - 17 (source: NCBI BLink). )
HSP 1 Score: 200.3 bits (508), Expect = 2.3e-51
Identity = 132/310 (42.58%), Postives = 192/310 (61.94%), Query Frame = 0
Query: 7 DSVHARLEKLRIVRENEIPVQQQKMESF-ADSFCKSVESINVRSRANALSQGKLGELKAR 66
D+ + L ++ E +I Q+ K++SF A F +S+ S+ R++A A SQ +L LKA
Sbjct: 11 DTTKETMASLGLICEKDIHEQRLKIDSFIASPFRRSMNSLVERAQATAQSQVELMNLKAD 70
Query: 67 LREAEDELVKALAVKTRKEAKRLTIIDSIATSKSRIEELRNTLQDCKARRDEYATIISQQ 126
LREAEDELVK LAVKTRKEA+++ I DSI+ ++SRIE LR LQ K+++D+ IISQQ
Sbjct: 71 LREAEDELVKVLAVKTRKEARQMGIRDSISATQSRIEVLRRNLQLQKSKKDDSVRIISQQ 130
Query: 127 SLALSSSERKETQESECRNEIQEAISWYKRVLDFHIEGGYGVKFSFKKINIDNPDEEYSF 186
ALS S+ + +E + +I EAISWY L FH+E G+GVKF+F I+ P E+SF
Sbjct: 131 LQALSKSKDNAGKVTEDKADIHEAISWYNHALGFHVEAGHGVKFTFTNIDAKRPTREFSF 190
Query: 187 TIRHANDTYMLLDCNPSLPDIKELIHELNKTNGLFKLVRIMRQRFQEAAAEGAGAPALSL 246
T+ + ND Y LLD + L I E++ ELNKTN LF+ VR+MR++F ++ + L
Sbjct: 191 TVHYGNDIYTLLDSDLQLDYINEMVQELNKTNDLFRFVRLMREQFLKSTLSELPTHSGQL 250
Query: 247 HQGSTIISMSAPGLSSSSERSESSTEEINKDEVEETNKHSKNILPRKKPAIHSSGSAFSL 306
Q ++ IS SAP +S S++ + S+ E NK + N+ K + + + S +
Sbjct: 251 QQETSAISASAPAISFSTDTNMSTPE--NKRSKVQVNRRQKR---GSESPLLAPVSTSAT 310
Query: 307 RRSPRFKVRK 316
RRS RFK +K
Sbjct: 311 RRSSRFKGKK 315
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q93VK9 | 3.3e-50 | 42.58 | Kinetochore protein SPC25 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC25 PE=1 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3CM71 | 3.2e-157 | 96.19 | Kinetochore protein SPC25 OS=Cucumis melo OX=3656 GN=LOC103502389 PE=3 SV=1 | [more] |
A0A0A0LU84 | 8.1e-153 | 94.29 | Kinetochore protein SPC25 OS=Cucumis sativus OX=3659 GN=Csa_1G004230 PE=3 SV=1 | [more] |
A0A5D3BFE9 | 5.4e-149 | 82.69 | Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... | [more] |
A0A5A7UUK0 | 1.6e-148 | 82.42 | Kinetochore protein SPC25 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... | [more] |
A0A6J1BYG5 | 1.6e-132 | 82.91 | Kinetochore protein SPC25 OS=Momordica charantia OX=3673 GN=LOC111006883 PE=3 SV... | [more] |
Match Name | E-value | Identity | Description | |
XP_008464538.1 | 6.6e-157 | 96.19 | PREDICTED: probable kinetochore protein SPC25 [Cucumis melo] | [more] |
XP_004138099.1 | 1.7e-152 | 94.29 | kinetochore protein SPC25 homolog [Cucumis sativus] >KGN63556.1 hypothetical pro... | [more] |
TYJ98520.1 | 1.1e-148 | 82.69 | putative kinetochore protein SPC25 [Cucumis melo var. makuwa] | [more] |
KAA0057836.1 | 3.3e-148 | 82.42 | putative kinetochore protein SPC25 [Cucumis melo var. makuwa] | [more] |
XP_038880541.1 | 1.4e-143 | 90.19 | kinetochore protein SPC25 homolog [Benincasa hispida] | [more] |
Match Name | E-value | Identity | Description | |
AT3G48210.1 | 2.3e-51 | 42.58 | CONTAINS InterPro DOMAIN/s: Kinetochore-Ndc80 complex, subunit Spc25 (InterPro:I... | [more] |