PI0020304 (gene) Melon (PI 482460) v1

Overview


Name	PI0020304
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	(R)-mandelonitrile lyase 1-like
Location	chr05: 5202731 .. 5207106 (-)
RNA-Seq Expression	PI0020304
Synteny	PI0020304

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCCTTTTCCTACTCATACTTACTCTATCTACCTTTCAATTCCAAATTGGTGTCCTCTCCTCACAAACTATACCAAATCAAGGTATATTTCTTTCTTTTATTTCACTTATTGAGTAGAGCTTATAAAACTTTAAAATGGTTTATTCGATAACCATTTCGTTTTTAATATCTCGTTATTTTAAATTAAACTTATAAATAAATACACATTTGTTATTCGGTTTCTAAAAATGTTTTTAAAATTCAAGGTTGAATTCTTAAAACTATTTTTTTTTAAATTGACTAAGAAATCAAATGTCTTCCAACTAGAGTAAAAATTACTCATGATAAGAAAATTTTATGATAACATGTAAATATAAATTTAAATAATAATAAACTAAACATGAAATTACTATCAAATGAGATTTTGGCTTCTAAACATGTTTGTGTGTAAAATTTTCATTCTAAAATTTGGGATTTTTTAATTAAAGAATTAGTCATTTTATAATCTTTTCTATTTTTTTTTTTTGGGTGAAATTTCAAATTTAGTCCTAATATTAGATCCTTATTTTTAGTTTTATGTCAAGAAAAATAAATTTCTACATTTAAAATACCAATACTAGACAGTAGACGCAAATCTTGTAGACATAAATTCAATTTCATTTTCTAAATTACGAATATGTCTAAGATATATCATATACTAAACATCAATTTAAAAATTGAGAACCTTTTATACACAAATTTGAAAGTTAGGACCAAATTTGTAACTTGACTTTTTGTTCTTGTTTTTTTGAAATCTAAGATATCAAATTAACAATTGACAACTCCCAGCTATCATTTTTTTTTCTTTGGTTGTCTTAGTATTTTTTCTCTAACTAATTATCATCCAATATTTTTCTTTTAGACGATAGTTACATAAAGTTTGTACAAAACGCAAATACTTTACCAACAACAGAAAAATACGACTACATAATAATAGGAGGAGGAACCGCCGGTTGCCCATTAGCTGCAACATTATCATCAAATTTCTCCGTCCTTGTTCTTGAAAGAGGAAGTGACCCCAATGCCTTCTCCATGGTCCTATCCCAAGAAGGCATGGCAAACACTCTCACCGACGATGACGACGGTCACAACCCATTCCAACGATTCGTTTCAGAAGATGGTGTCGAGAACATTAGAGGTCGAGTCCTTGGTGGAGGTAGCATGATCAACGTGGGATTCTACTCAAGAGCACAACCAGAGTTCTTCAAAAACTCGAGTGTCCAATGGGACATGGCGATGGTAGAGGAGGCATATCGGTGGATCGAAGAGACGGTCGTGTCTCGACCAGAGTTGGGGCCTTGGCAATTGGCTTTCAAGGAAGCACTTGTGGAAGCTGGGGTTGGCCCTGATAATGGATATGATTTGGATCATGTTTTTGGGACAAGAATTGGAGGTTCTATCTTTGATTCTAGAGGAAAAAGGCATGGAGCTTTGGAGCTTCTTAATAAGGCTAATCCTATAAATCTTAAAGTTGCCACCCAAGCCACAGTCAAAAGAATCATTTTCTCTCAATCCAATGGTGGGTATAGAACAATATCCCTTTCATATATCTCTTTTATGTTTGAATATTTCATTAAAATGTTCCCTTTTTACTCCTATAATTAATTAGTTAGCTTAAATAAATAAATATATTCGTCGTTTATAAATATAGCAAATTGTCACTATCTATTATTGGTAGATACTGATAGACACCTTATAGACTACTATCCTTTTATCACTGATAGGATAATTAAAAACATTTAAATATTTTCAACAGTTTTGCTATTAAAAATTTATTAAATAGTTCTCTCGTGAGAGTTCTTGAGTTATTTATTTATTTATTATTTTTGTTAAAAATAACATTTTGGTCTCCTACTCTAAAGTTTATTCAATTTTATTCCCTATATATTCAGATTTTTAATTTTAGTCACTACATTTTCAATAAATCTTTTACCTTTTATATTTATTAGCATTTTCATTTTAAAATTTTAAAACATATTCATACATTATTGTATTTTTCTTTCATGAAAATTGTTATTATTATTTAATAAATTTTGGTAAAAATTAAACTTTAGGAACTAAATTTCAAATTTTACTAAAAGTGAGGACTAAAATAAGACATTGAAAAGTACATGAATAAATTACAGACTATATAAGAATCAAAATGATATTTTAACTTTTGTTTTTATTATTGTTTTTCAACATATGTCATTTTATAAGCTCATTACATCTTTCGACCTTGTCCTAAATTCCTCACCTAAAATCAATCTACATGTTAAATCAAACGATATAAACCACAAAATTTTTCTTCTTAACCAATCATTCCTTTGAATCTTCTAGGTTTATCAGCAACTGGGGTTTTATATTCCGATTCAAATGGCAAACTTCATACAGCAACCATCTCTAATAACGGAGAAATCATTCTAAGTGCAGGCGCTATTGGAAGTCCTCAACTTCTTCTCTCAAGTGGGGTTGGTCCAAAGTCTCATCTTTCATCCTTAAAACTACCTCTCGTTCTTCACAATCGACATGTCGGTCAATCCATGGCTGACAACCCGCGCTTCGGTGCTGCCATCGTCCTCCCATTTCTCACCTCGCCAACGTCGGTACAAGTCGTTGGAACTTTGAAACCTAACATCCACATTGAATCTCTCTCAACTATTTTACCCTTTTCAATCTCCCCACCTTTCGCTCTTCTTCCTCCTCGTTCCTCCACGGTTAATCTCAGCGTTGCTATCTTCGCCGGAAAATTCTCTACCGTGTCCTCCACCGGAAGCCTCCGGCTGGACAGAAGAAAAAACCCGATCGTTCGATTTAATTACTTATCACATCCGGATGATCTCGAGAGGTGTGTTGAAGGGGTTAGAAAAGTTGGAGAATTGGTGAATACAAAAGTTATGGAGAGGATCAAGACGAGGAATTTAGAGGGTAAAATGGGGTTTGAGTTTTTGGGGAGTTCGTTGCCGAAAAACATGTCAGATTATGGTTTGGTAGGAGAGTTTTGTCGGAAAACAGTGACGACATTTTGGCATTACCATGGAGGATGTTTGGTTGGAAAAGTTGTCGACAGTAATTATAAAGTCATTGGAATTAATAACCTTCGTGTCGTCGATGGTTCTACTTTTTCTCTCTCGCCGGGAACAAATCCTATGGCCACCGTTATGATGTTGGGCAGGTGCGTCTTCTTATTTACTTTTTTTTTTTCTTTTTTCAAAACAAGTTTTAAAAAGAATTTTGCCACTAAAATATTATTGTTTATATAAATAGTGATCGATAGCAATAATAAATCTTATAATAATATTTGTAAATAATTTAACTCGTTTTTGTCGTATTTGAAAACATTCAAACTTTTATATCTAAACGAAAGACATACATGGAAAATACATCTATTACTTTCTTTAATCATATAATATACATATATACATATATATTAATTTAATAAAAGCAGATGAACATCTAAGAATAAGTAGACTGTAGTTAAATTATGTGGGGTAGATTTGACATTTTCAATTATATAAAACTCAAACTGGTCCAAAGTGATTTTCAACTGTCTCATTTCTTTTCTATATATATATATCATTTTTTAGTCAAGACATGATTACTTCAACTAGATCTAAAAGTCAAAGTGATCTAAAAGTGATTTTCAAACCAACTCAATTTGTTTTATTTTATTTTATTTAAAAAAATAAGAATCAAATACTTTGGATGGCTATTTGAATCAACGAAATACAGTAGGTGTGAATAAGTTCGGCAAAATTTAACCACTTAATGTTTTTTACTACTGTCCTCTCAGTCAAGAACTTTACTTGAATCTACTTCTCACATATTGGGTTGCAATGTGTCTCTGAAGTATACTTTTGGCCACTAAAATATCAAATTCAAATTTGTTAGAATTATTGTGTGGGTTATTTTTAAATATATAGCAAACTTAACCTAAACATTTACAAAATATAATAAAATATTATCATTTATTTATGATGGATCGCAATAGACCAAGATAGACTACTACATGTATCTATCTATATGATGATATACATGGATAGTAGTCTATCTTCAGTCTATTATAAATAAATTGAGATATTTTGCTATATTTAAAAATATTTTTAGAAGTTTTTCATTTAAAATAATTTTTCATATTATTATTGTTATTATTATTATTTTGTGAAAGGTATTAAAATCTATTATTATAAGATTTAAGATTTTCTTTTGGCTTTCAATAGTTTCTAGCTTTTAATTATTGCCTATATATATTTCTTGATATTATTATTTATTTATATATTGAGATTATATTTTGAAAATGTTTTTGCAGGTATGTTGGCCTTAAGATGTTGCATCAAAGATTGGGTCAAAGATCCAAACCATTTGGCAATTGA

mRNA sequence

ATGGCTTCCCTTTTCCTACTCATACTTACTCTATCTACCTTTCAATTCCAAATTGGTGTCCTCTCCTCACAAACTATACCAAATCAAGACGATAGTTACATAAAGTTTGTACAAAACGCAAATACTTTACCAACAACAGAAAAATACGACTACATAATAATAGGAGGAGGAACCGCCGGTTGCCCATTAGCTGCAACATTATCATCAAATTTCTCCGTCCTTGTTCTTGAAAGAGGAAGTGACCCCAATGCCTTCTCCATGGTCCTATCCCAAGAAGGCATGGCAAACACTCTCACCGACGATGACGACGGTCACAACCCATTCCAACGATTCGTTTCAGAAGATGGTGTCGAGAACATTAGAGGTCGAGTCCTTGGTGGAGGTAGCATGATCAACGTGGGATTCTACTCAAGAGCACAACCAGAGTTCTTCAAAAACTCGAGTGTCCAATGGGACATGGCGATGGTAGAGGAGGCATATCGGTGGATCGAAGAGACGGTCGTGTCTCGACCAGAGTTGGGGCCTTGGCAATTGGCTTTCAAGGAAGCACTTGTGGAAGCTGGGGTTGGCCCTGATAATGGATATGATTTGGATCATGTTTTTGGGACAAGAATTGGAGGTTCTATCTTTGATTCTAGAGGAAAAAGGCATGGAGCTTTGGAGCTTCTTAATAAGGCTAATCCTATAAATCTTAAAGTTGCCACCCAAGCCACAGTCAAAAGAATCATTTTCTCTCAATCCAATGGTTTATCAGCAACTGGGGTTTTATATTCCGATTCAAATGGCAAACTTCATACAGCAACCATCTCTAATAACGGAGAAATCATTCTAAGTGCAGGCGCTATTGGAAGTCCTCAACTTCTTCTCTCAAGTGGGGTTGGTCCAAAGTCTCATCTTTCATCCTTAAAACTACCTCTCGTTCTTCACAATCGACATGTCGGTCAATCCATGGCTGACAACCCGCGCTTCGGTGCTGCCATCGTCCTCCCATTTCTCACCTCGCCAACGTCGGTACAAGTCGTTGGAACTTTGAAACCTAACATCCACATTGAATCTCTCTCAACTATTTTACCCTTTTCAATCTCCCCACCTTTCGCTCTTCTTCCTCCTCGTTCCTCCACGGTTAATCTCAGCGTTGCTATCTTCGCCGGAAAATTCTCTACCGTGTCCTCCACCGGAAGCCTCCGGCTGGACAGAAGAAAAAACCCGATCGTTCGATTTAATTACTTATCACATCCGGATGATCTCGAGAGGTGTGTTGAAGGGGTTAGAAAAGTTGGAGAATTGGTGAATACAAAAGTTATGGAGAGGATCAAGACGAGGAATTTAGAGGGTAAAATGGGGTTTGAGTTTTTGGGGAGTTCGTTGCCGAAAAACATGTCAGATTATGGTTTGGTAGGAGAGTTTTGTCGGAAAACAGTGACGACATTTTGGCATTACCATGGAGGATGTTTGGTTGGAAAAGTTGTCGACAGTAATTATAAAGTCATTGGAATTAATAACCTTCGTGTCGTCGATGGTTCTACTTTTTCTCTCTCGCCGGGAACAAATCCTATGGCCACCGTTATGATGTTGGGCAGGTATGTTGGCCTTAAGATGTTGCATCAAAGATTGGGTCAAAGATCCAAACCATTTGGCAATTGA

Coding sequence (CDS)

ATGGCTTCCCTTTTCCTACTCATACTTACTCTATCTACCTTTCAATTCCAAATTGGTGTCCTCTCCTCACAAACTATACCAAATCAAGACGATAGTTACATAAAGTTTGTACAAAACGCAAATACTTTACCAACAACAGAAAAATACGACTACATAATAATAGGAGGAGGAACCGCCGGTTGCCCATTAGCTGCAACATTATCATCAAATTTCTCCGTCCTTGTTCTTGAAAGAGGAAGTGACCCCAATGCCTTCTCCATGGTCCTATCCCAAGAAGGCATGGCAAACACTCTCACCGACGATGACGACGGTCACAACCCATTCCAACGATTCGTTTCAGAAGATGGTGTCGAGAACATTAGAGGTCGAGTCCTTGGTGGAGGTAGCATGATCAACGTGGGATTCTACTCAAGAGCACAACCAGAGTTCTTCAAAAACTCGAGTGTCCAATGGGACATGGCGATGGTAGAGGAGGCATATCGGTGGATCGAAGAGACGGTCGTGTCTCGACCAGAGTTGGGGCCTTGGCAATTGGCTTTCAAGGAAGCACTTGTGGAAGCTGGGGTTGGCCCTGATAATGGATATGATTTGGATCATGTTTTTGGGACAAGAATTGGAGGTTCTATCTTTGATTCTAGAGGAAAAAGGCATGGAGCTTTGGAGCTTCTTAATAAGGCTAATCCTATAAATCTTAAAGTTGCCACCCAAGCCACAGTCAAAAGAATCATTTTCTCTCAATCCAATGGTTTATCAGCAACTGGGGTTTTATATTCCGATTCAAATGGCAAACTTCATACAGCAACCATCTCTAATAACGGAGAAATCATTCTAAGTGCAGGCGCTATTGGAAGTCCTCAACTTCTTCTCTCAAGTGGGGTTGGTCCAAAGTCTCATCTTTCATCCTTAAAACTACCTCTCGTTCTTCACAATCGACATGTCGGTCAATCCATGGCTGACAACCCGCGCTTCGGTGCTGCCATCGTCCTCCCATTTCTCACCTCGCCAACGTCGGTACAAGTCGTTGGAACTTTGAAACCTAACATCCACATTGAATCTCTCTCAACTATTTTACCCTTTTCAATCTCCCCACCTTTCGCTCTTCTTCCTCCTCGTTCCTCCACGGTTAATCTCAGCGTTGCTATCTTCGCCGGAAAATTCTCTACCGTGTCCTCCACCGGAAGCCTCCGGCTGGACAGAAGAAAAAACCCGATCGTTCGATTTAATTACTTATCACATCCGGATGATCTCGAGAGGTGTGTTGAAGGGGTTAGAAAAGTTGGAGAATTGGTGAATACAAAAGTTATGGAGAGGATCAAGACGAGGAATTTAGAGGGTAAAATGGGGTTTGAGTTTTTGGGGAGTTCGTTGCCGAAAAACATGTCAGATTATGGTTTGGTAGGAGAGTTTTGTCGGAAAACAGTGACGACATTTTGGCATTACCATGGAGGATGTTTGGTTGGAAAAGTTGTCGACAGTAATTATAAAGTCATTGGAATTAATAACCTTCGTGTCGTCGATGGTTCTACTTTTTCTCTCTCGCCGGGAACAAATCCTATGGCCACCGTTATGATGTTGGGCAGGTATGTTGGCCTTAAGATGTTGCATCAAAGATTGGGTCAAAGATCCAAACCATTTGGCAATTGA

Protein sequence

MASLFLLILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVKRIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFSISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLGQRSKPFGN

Homology

[Close]

BLAST of PI0020304 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 5.7e-143
Identity = 254/519 (48.94%), Postives = 350/519 (67.44%), Query Frame = 0


Query: 28  NQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFSM 87
           N+   +++F+ NA    + + YDYII+GGGTAGCPLAATLS +F VL+LERG  P     
Sbjct: 33  NRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPN 92

Query: 88  VLSQEGMANTLTDDDDGHNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNS 147
           V+S +G   TLTD ++  +P Q F+SE+GV N RGRVLGG S IN GFYSRA  +FF+NS
Sbjct: 93  VMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENS 152

Query: 148 SVQWDMAMVEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLDHVFGTRIGG 207
            + WD++ V ++Y W+E  +V RP+L  WQ A ++AL+E GV P NG+ L+H  GT+IGG
Sbjct: 153 GLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGG 212

Query: 208 SIFDSRGKRHGALELLNKANPINLKVATQATVKRIIFSQS-----NGLSATGVLYSDSNG 267
           S FD  G+RH + +LL  A   N++VA  ATV+R++ + S     + +SA GV+Y D  G
Sbjct: 213 STFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLG 272

Query: 268 KLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPLVLHNRHVGQSMADNPR 327
           + H A I + GE+ILSAGA+GSPQLL  SG+GP+S+LS+  +P+ L   HVG  + DNPR
Sbjct: 273 RFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPR 332

Query: 328 FGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFSISPPFALLPPRSSTVNLSVAIF 387
            G +IV P     + +QVVG  +    +E+ S ++PF+       +   +S + + V   
Sbjct: 333 NGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTI 392

Query: 388 AGKFSTVSSTGSLRL---DRRKNPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVMERIK 447
             K     S G LRL   D R NP+VRFNY S P DLERCV G RK+GE++ ++ M+   
Sbjct: 393 MEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFM 452

Query: 448 TRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDSNYKVIGI 507
            R   G   F F+G+ LP + S+  ++ +FCR+TV+T WHYHGG +VGKVVDS+ KVIG+
Sbjct: 453 IREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGV 512

Query: 508 NNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRL 539
           N+LR+VDGSTF++SPGTNP AT+MMLGRY+GLKML +R+
Sbjct: 513 NSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERM 551

[Close]

BLAST of PI0020304 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 3.7e-142
Identity = 268/541 (49.54%), Postives = 355/541 (65.62%), Query Frame = 0


Query: 3   SLFLLILTLSTFQFQIG-VLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAGC 62
           S  LL+L L     Q   V S  T  N D SY++F  +A  L     YDY+I+GGGT+GC
Sbjct: 7   SAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGC 66

Query: 63  PLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENIR 122
           PLAATLS  + VLVLERGS P A+  VL+ +G    L  +DDG  P +RFVSEDG++N+R
Sbjct: 67  PLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVR 126

Query: 123 GRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAFK 182
           GRVLGG SMIN G Y+RA    +  S V WDM +V + Y W+E+T+V +P   PWQ    
Sbjct: 127 GRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTG 186

Query: 183 EALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVKR 242
            A +EAGV P++G+ LDH  GTRI GS FD++G RH A ELLNK N  NL+V   A+V++
Sbjct: 187 TAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEK 246

Query: 243 IIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLSS 302
           IIFS + GL+ATGV+Y DSNG  H A + + GE+I+SAG IG+PQLLL SGVGP+S+LSS
Sbjct: 247 IIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSS 306

Query: 303 LKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFSI 362
           L +P+VL + +VGQ + DNPR    I+ P    PT V V+G    N   +   + LPF+ 
Sbjct: 307 LNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFT- 366

Query: 363 SPPFALLPPRSSTV-NLSVAIFAGKFSTVSSTGSLRLDRRKN----PIVRFNYLSHPDDL 422
           +PPF+  P  S  + N + A FA K +   S GSL L    N    P V+FNY S+P DL
Sbjct: 367 TPPFSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDL 426

Query: 423 ERCVEGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTT 482
             CV G++K+GEL++T  ++  K  +L G  GF  LG  LPK+ +D      FCR++V +
Sbjct: 427 SHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVAS 486

Query: 483 FWHYHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQ 538
           +WHYHGGCLVGKV+D +++V GI+ LRVVDGSTF  +P ++P    +MLGRYVG+K+L +
Sbjct: 487 YWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 544

[Close]

BLAST of PI0020304 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 5.9e-140
Identity = 265/541 (48.98%), Postives = 352/541 (65.06%), Query Frame = 0


Query: 3   SLFLLILTLSTFQFQIG-VLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAGC 62
           S  LL+L +     Q   V S  T  + D SY+ F  +A  L     YDY+I+GGGT+GC
Sbjct: 7   SAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSGC 66

Query: 63  PLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENIR 122
           PLAATLS  + VLVLERGS P A+  VL+ +G    L  +DDG  P +RFVSEDG++N+R
Sbjct: 67  PLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVR 126

Query: 123 GRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAFK 182
           GRVLGG S+IN G Y+RA    +  S V WDM +V + Y W+E+T+V +P    WQ   K
Sbjct: 127 GRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTK 186

Query: 183 EALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVKR 242
            A +EAGV P++G+ LDH  GTRI GS FD++G RH A ELLNK N  NL+V   A+V++
Sbjct: 187 TAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEK 246

Query: 243 IIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLSS 302
           IIFS + GL+ATGV+Y DSNG  H A + + GE+I+SAG IG+PQLLL SGVGP+S+LSS
Sbjct: 247 IIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSS 306

Query: 303 LKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFSI 362
           L +P+VL + +VGQ + DNPR    I+ P    PT V V+G    N   +   + LPF+ 
Sbjct: 307 LNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFT- 366

Query: 363 SPPFALLPPRSSTV-NLSVAIFAGKFSTVSSTGSLRLDRRKN----PIVRFNYLSHPDDL 422
           +PPF   P  S  + N + A FA K +   S GSL L    N    P V+FNY S+  DL
Sbjct: 367 TPPFGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDL 426

Query: 423 ERCVEGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTT 482
             CV G++K+GEL++T  ++  K  +L G  GF  LG  LPK+ +D      FCR++V +
Sbjct: 427 SHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVAS 486

Query: 483 FWHYHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQ 538
           +WHYHGGCLVGKV+D +++V GIN LRVVDGSTF  +P ++P    +MLGRYVG+K+L +
Sbjct: 487 YWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 544

[Close]

BLAST of PI0020304 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.8e-136
Identity = 261/543 (48.07%), Postives = 353/543 (65.01%), Query Frame = 0


Query: 3   SLFLLILTLSTFQFQIGVLSS--QTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAG 62
           S  +L+L L     Q   + S   T    D  Y+KFV NA  L     YDYII+GGGT+G
Sbjct: 7   SAVVLVLNLLVLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIVGGGTSG 66

Query: 63  CPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENI 122
           CPLAATLS+N+SVLVLERG+    +   L+ +G A  L   DDG  P +RFVSEDG++N+
Sbjct: 67  CPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSEDGIDNV 126

Query: 123 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAF 182
           R R+LGG ++IN G Y+RA   F+ NS V+WD+ +V EAY W+E+ +V +P    WQ   
Sbjct: 127 RSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQSWQSIT 186

Query: 183 KEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVK 242
             A +EAGV PDNG+ L H  GTR+ GS FD+ G RH + ELLNK +P NLKVA +A V+
Sbjct: 187 GTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVAVEAAVQ 246

Query: 243 RIIFS-QSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHL 302
           +IIFS +S+GL+A GV+Y+DSNG  H A +S  GE+ILSAG +G+PQLLL SGVGP+S+L
Sbjct: 247 KIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGVGPESYL 306

Query: 303 SSLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPF 362
           +SL + +V  + +VGQ + DNPR    I+ P    P++V V+G +  + +  SLS+ LPF
Sbjct: 307 TSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLG-ITSDFYQCSLSS-LPF 366

Query: 363 SISPPFALLPPRSSTV-NLSVAIFAGKFSTVSSTGSLRLDRRKN----PIVRFNYLSHPD 422
             +PPF+L P  S  + N + A    K     S GSL L    N    P V+FNY S P 
Sbjct: 367 D-TPPFSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFNYCSDPV 426

Query: 423 DLERCVEGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTV 482
           DL  CV G++K+G  ++T  ++  K  +L G  GF  LG+ LP+N +D     +FCR TV
Sbjct: 427 DLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEKFCRDTV 486

Query: 483 TTFWHYHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKML 538
            ++WHYHGG +VGKV+D N++V GIN LRVVDGSTF  +P ++P    +MLGRYVG K++
Sbjct: 487 ASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLGRYVGTKIV 546

[Close]

BLAST of PI0020304 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 4.8e-134
Identity = 256/542 (47.23%), Postives = 353/542 (65.13%), Query Frame = 0


Query: 3   SLFLLILTLSTFQFQIG-VLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAGC 62
           S  LL+L +     Q   V S     + D SY+ FV +A        YDYII+GGGTAGC
Sbjct: 7   SAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGC 66

Query: 63  PLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENIR 122
           PLAATLS+N+SVLVLERGS P  +  +L  +G    L  +DDG  P +RFVSEDG++N+R
Sbjct: 67  PLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVR 126

Query: 123 GRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAFK 182
           GRVLGG SMIN G Y RA   FF  + ++WDM +V + Y W+E+T+V  P+   WQ    
Sbjct: 127 GRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIG 186

Query: 183 EALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVKR 242
            A +EAG+ P+NG+ +DH+ GTR+ GS FD+ G RH + ELLNK +P NL+VA QA V++
Sbjct: 187 TAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEK 246

Query: 243 IIF-SQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLS 302
           IIF S ++G++A GV+Y+DSNG  H A +   GE+ILSAG IGSPQLLL SGVGP+S+L+
Sbjct: 247 IIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLT 306

Query: 303 SLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFS 362
           SL + +V  + +VGQ + DNPR    I+ P     ++V V+G +  + +  S+S+ LPF 
Sbjct: 307 SLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLG-ITSDFYQCSISS-LPFD 366

Query: 363 ISPPFALLPPRSSTV-NLSVAIFAGKFSTVSSTGSLRL----DRRKNPIVRFNYLSHPDD 422
            +PPF+  P  S  + N + A    K     S G++ L    D R  P V+FNY S+  D
Sbjct: 367 -TPPFSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTD 426

Query: 423 LERCVEGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVT 482
           L  CV G++K+GE+++T  +E  K  +L G  GF  LG  LP+N +D      FCR++V 
Sbjct: 427 LSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVA 486

Query: 483 TFWHYHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLH 538
           ++WHYHGGCLVGKV+D  ++V GIN LRVVDGSTF  +P ++P    +MLGRY+G+++L 
Sbjct: 487 SYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQ 545

[Close]

BLAST of PI0020304 vs. ExPASy TrEMBL
Match: A0A5A7UKP1 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G001010 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 9.0e-301
Identity = 526/548 (95.99%), Postives = 535/548 (97.63%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVL-SSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTA 60
           MASLFLLIL L TFQFQIGVL SSQTIPNQDDSYIKF+QNANTLPTTEKYDYIIIGGGTA
Sbjct: 1   MASLFLLILALFTFQFQIGVLISSQTIPNQDDSYIKFIQNANTLPTTEKYDYIIIGGGTA 60

Query: 61  GCPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVEN 120
           GCPLAATLSSNFSVLVLERGSDPNAF MVLSQEGMANTLTDDD+GHNPFQRF+SEDGVEN
Sbjct: 61  GCPLAATLSSNFSVLVLERGSDPNAFPMVLSQEGMANTLTDDDNGHNPFQRFISEDGVEN 120

Query: 121 IRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLA 180
           IRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLA
Sbjct: 121 IRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLA 180

Query: 181 FKEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATV 240
           FKEALVEAGVGPDNGYDL+HV GTRIGGSIFDSRGKRHGA+ELLNKANPINLKVATQATV
Sbjct: 181 FKEALVEAGVGPDNGYDLNHVVGTRIGGSIFDSRGKRHGAVELLNKANPINLKVATQATV 240

Query: 241 KRIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHL 300
           KRIIFSQSNGLSATGVLYSDSNGKLH ATIS NGEIILSAGAIGSPQLLLSSGVGPKSHL
Sbjct: 241 KRIIFSQSNGLSATGVLYSDSNGKLHKATISKNGEIILSAGAIGSPQLLLSSGVGPKSHL 300

Query: 301 SSLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPF 360
           SSLKLPLVLHNRHVGQSMADNPRFGA +VLPFLT PTSVQVVGTLKPNIHIESLSTILPF
Sbjct: 301 SSLKLPLVLHNRHVGQSMADNPRFGATVVLPFLTRPTSVQVVGTLKPNIHIESLSTILPF 360

Query: 361 SISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERC 420
           SISPPFALLPPRSS VNLS+AIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERC
Sbjct: 361 SISPPFALLPPRSSGVNLSLAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERC 420

Query: 421 VEGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWH 480
           VEGVRKVGELVNTKVMERIKTR+LEGKMGFEFLG SLP+NMSDYGLVGEFCRKTVTTFWH
Sbjct: 421 VEGVRKVGELVNTKVMERIKTRDLEGKMGFEFLGRSLPENMSDYGLVGEFCRKTVTTFWH 480

Query: 481 YHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLG 540
           YHGGCLVGKVVD NYKVIGINNLRVVDGSTFSLSPGTNPMA VMMLGRYVGLKMLHQRLG
Sbjct: 481 YHGGCLVGKVVDGNYKVIGINNLRVVDGSTFSLSPGTNPMAAVMMLGRYVGLKMLHQRLG 540

Query: 541 QRSKPFGN 548
           QRSKPFGN
Sbjct: 541 QRSKPFGN 548

[Close]

BLAST of PI0020304 vs. ExPASy TrEMBL
Match: A0A1S3BTC3 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493044 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 9.0e-301
Identity = 526/548 (95.99%), Postives = 535/548 (97.63%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVL-SSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTA 60
           MASLFLLIL L TFQFQIGVL SSQTIPNQDDSYIKF+QNANTLPTTEKYDYIIIGGGTA
Sbjct: 1   MASLFLLILALFTFQFQIGVLISSQTIPNQDDSYIKFIQNANTLPTTEKYDYIIIGGGTA 60

Query: 61  GCPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVEN 120
           GCPLAATLSSNFSVLVLERGSDPNAF MVLSQEGMANTLTDDD+GHNPFQRF+SEDGVEN
Sbjct: 61  GCPLAATLSSNFSVLVLERGSDPNAFPMVLSQEGMANTLTDDDNGHNPFQRFISEDGVEN 120

Query: 121 IRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLA 180
           IRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLA
Sbjct: 121 IRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLA 180

Query: 181 FKEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATV 240
           FKEALVEAGVGPDNGYDL+HV GTRIGGSIFDSRGKRHGA+ELLNKANPINLKVATQATV
Sbjct: 181 FKEALVEAGVGPDNGYDLNHVVGTRIGGSIFDSRGKRHGAVELLNKANPINLKVATQATV 240

Query: 241 KRIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHL 300
           KRIIFSQSNGLSATGVLYSDSNGKLH ATIS NGEIILSAGAIGSPQLLLSSGVGPKSHL
Sbjct: 241 KRIIFSQSNGLSATGVLYSDSNGKLHKATISKNGEIILSAGAIGSPQLLLSSGVGPKSHL 300

Query: 301 SSLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPF 360
           SSLKLPLVLHNRHVGQSMADNPRFGA +VLPFLT PTSVQVVGTLKPNIHIESLSTILPF
Sbjct: 301 SSLKLPLVLHNRHVGQSMADNPRFGATVVLPFLTRPTSVQVVGTLKPNIHIESLSTILPF 360

Query: 361 SISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERC 420
           SISPPFALLPPRSS VNLS+AIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERC
Sbjct: 361 SISPPFALLPPRSSGVNLSLAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERC 420

Query: 421 VEGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWH 480
           VEGVRKVGELVNTKVMERIKTR+LEGKMGFEFLG SLP+NMSDYGLVGEFCRKTVTTFWH
Sbjct: 421 VEGVRKVGELVNTKVMERIKTRDLEGKMGFEFLGRSLPENMSDYGLVGEFCRKTVTTFWH 480

Query: 481 YHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLG 540
           YHGGCLVGKVVD NYKVIGINNLRVVDGSTFSLSPGTNPMA VMMLGRYVGLKMLHQRLG
Sbjct: 481 YHGGCLVGKVVDGNYKVIGINNLRVVDGSTFSLSPGTNPMAAVMMLGRYVGLKMLHQRLG 540

Query: 541 QRSKPFGN 548
           QRSKPFGN
Sbjct: 541 QRSKPFGN 548

[Close]

BLAST of PI0020304 vs. ExPASy TrEMBL
Match: A0A0A0LMC2 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G057070 PE=3 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.2e-299
Identity = 522/547 (95.43%), Postives = 533/547 (97.44%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAG 60
           MASLFLLIL L TFQ QI VL+SQTIPNQDDSYIKF+QNANTLPTTEKYDYIIIGGGTAG
Sbjct: 1   MASLFLLILALFTFQLQISVLTSQTIPNQDDSYIKFIQNANTLPTTEKYDYIIIGGGTAG 60

Query: 61  CPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENI 120
           CPLAATLSSNFSVLVLERGSDPNAF MVLSQEGMANTLT+DDDGHNPFQRFVSEDGVENI
Sbjct: 61  CPLAATLSSNFSVLVLERGSDPNAFPMVLSQEGMANTLTEDDDGHNPFQRFVSEDGVENI 120

Query: 121 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAF 180
           RGRVLGGGSMINVGFYSRAQPEFFKNSSVQW+M MVEEAYRWIEETVVSRPELGPWQLAF
Sbjct: 121 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWNMEMVEEAYRWIEETVVSRPELGPWQLAF 180

Query: 181 KEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVK 240
           KEALVEAGVGPDNGYDLDHV GTRIGGSIFDSRGKRHGA+ELLNKANPINLKVATQATVK
Sbjct: 181 KEALVEAGVGPDNGYDLDHVVGTRIGGSIFDSRGKRHGAVELLNKANPINLKVATQATVK 240

Query: 241 RIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLS 300
           RIIFS+SNGLSATGVLYSDSNGKLH ATIS NGEIILSAGAIGSPQLLLSSGVGPKS LS
Sbjct: 241 RIIFSRSNGLSATGVLYSDSNGKLHKATISRNGEIILSAGAIGSPQLLLSSGVGPKSDLS 300

Query: 301 SLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFS 360
           SLKLPLVLHNRHVGQSMADNPR GAAIVLPFLT PTSVQVVGTLKPNIHIESLSTILPFS
Sbjct: 301 SLKLPLVLHNRHVGQSMADNPRLGAAIVLPFLTPPTSVQVVGTLKPNIHIESLSTILPFS 360

Query: 361 ISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV 420
           ISPPFALLPPRSS VNLS+AIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV
Sbjct: 361 ISPPFALLPPRSSAVNLSLAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV 420

Query: 421 EGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHY 480
           EGVRKVGELVNTKVMERIKTR+LEGKMGFEFLGSSLP+NMSDYGLVGEFCRKTVTTFWHY
Sbjct: 421 EGVRKVGELVNTKVMERIKTRDLEGKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHY 480

Query: 481 HGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLGQ 540
           HGGC+VGKVVD NYKVIG+NNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLGQ
Sbjct: 481 HGGCVVGKVVDGNYKVIGVNNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLGQ 540

Query: 541 RSKPFGN 548
           RSKPFGN
Sbjct: 541 RSKPFGN 547

[Close]

BLAST of PI0020304 vs. ExPASy TrEMBL
Match: A0A6J1EN54 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436125 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.2e-263
Identity = 462/539 (85.71%), Postives = 493/539 (91.47%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAG 60
           MA+  LL+L   TFQ QI VL SQTIPNQDDSY+KFVQ ANTLPT E+YDYIIIGGGTAG
Sbjct: 1   MAASLLLLLLFFTFQCQIRVLFSQTIPNQDDSYMKFVQKANTLPTREEYDYIIIGGGTAG 60

Query: 61  CPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENI 120
           CPLAATLSSNFSVLVLERGSDPNAF  VLSQ+G+ANTLTD+DDG NPFQRFVSEDGVENI
Sbjct: 61  CPLAATLSSNFSVLVLERGSDPNAFPSVLSQQGLANTLTDNDDGLNPFQRFVSEDGVENI 120

Query: 121 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAF 180
           RGRVLGGGSMINVGFYSRAQPEFFK+S +QWDM  VE+AY+WIEETVVSRPEL PWQ AF
Sbjct: 121 RGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDMTAVEKAYQWIEETVVSRPELSPWQSAF 180

Query: 181 KEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVK 240
           +E L+EAGVGPDNGYDL H  GTR GGSIFDSRG+RHGA+ELLNKA+P NL+VATQATVK
Sbjct: 181 REVLLEAGVGPDNGYDLKHGVGTRTGGSIFDSRGRRHGAVELLNKADPRNLRVATQATVK 240

Query: 241 RIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLS 300
           RIIFSQSNGLSA+GVLYSD  GKLH ATIS NGEIIL+AGAIGSP LLL SGVGPKSHLS
Sbjct: 241 RIIFSQSNGLSASGVLYSDFKGKLHKATISKNGEIILTAGAIGSPHLLLQSGVGPKSHLS 300

Query: 301 SLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFS 360
           SLKLP+VLHNRHVGQSMADNPRFGAAIVLPFLT PTSVQVVGTLK NIHIESLS+ILPFS
Sbjct: 301 SLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLTPPTSVQVVGTLKRNIHIESLSSILPFS 360

Query: 361 ISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV 420
           I PPF LLPPRS+ VNLS+A+FAGKFSTVSS GSLRLD RKNPIVRFNYLSHPDD+ERCV
Sbjct: 361 IFPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVGSLRLDGRKNPIVRFNYLSHPDDVERCV 420

Query: 421 EGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHY 480
           EGVRKVG+LVNTK MERIKT +LEGK GF+FLGS LP+NMSDY LVG+FCRKTVTTFWHY
Sbjct: 421 EGVRKVGDLVNTKSMERIKTGDLEGKKGFKFLGSPLPENMSDYKLVGDFCRKTVTTFWHY 480

Query: 481 HGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLG 540
           HGGCLVGKVVD NY+VIGI  LRVVDGSTFSLSPGTNPMATVMMLGRYVGLKML QRLG
Sbjct: 481 HGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQRLG 539

[Close]

BLAST of PI0020304 vs. ExPASy TrEMBL
Match: A0A6J1K9L8 ((R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492348 PE=3 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 1.3e-262
Identity = 459/539 (85.16%), Postives = 495/539 (91.84%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAG 60
           MA+  LL+L L  FQ QI VL SQTIPNQDDSY+KFVQNANTLPT E+YDYIIIGGGTAG
Sbjct: 1   MAASLLLLLLLFIFQCQIPVLFSQTIPNQDDSYMKFVQNANTLPTREEYDYIIIGGGTAG 60

Query: 61  CPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENI 120
           CPLAATLSSNFSVLVLERGSDPNAF  VLSQ+G+ANTLTD+DDG NPFQRFVSEDGVENI
Sbjct: 61  CPLAATLSSNFSVLVLERGSDPNAFPSVLSQQGLANTLTDNDDGLNPFQRFVSEDGVENI 120

Query: 121 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAF 180
           RGRVLGGGSMINVGFYSRAQPEFF++S VQWDM  VE+AY+WIEETVVSRPEL PWQ AF
Sbjct: 121 RGRVLGGGSMINVGFYSRAQPEFFQSSGVQWDMMAVEKAYQWIEETVVSRPELSPWQSAF 180

Query: 181 KEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVK 240
           +EAL+EAGVG DNGYDL+HV GTR GGSIFDS G+RHGA+ELLNKA+P NL+VATQATVK
Sbjct: 181 REALLEAGVGRDNGYDLNHVVGTRTGGSIFDSSGRRHGAVELLNKADPRNLRVATQATVK 240

Query: 241 RIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLS 300
           RIIFSQSNGLSA+GVLYSD  GKLH ATIS NGEIIL+AGAIGSP LLL SGVGP+SHLS
Sbjct: 241 RIIFSQSNGLSASGVLYSDFKGKLHKATISRNGEIILTAGAIGSPHLLLQSGVGPRSHLS 300

Query: 301 SLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFS 360
           SLKLP+V HN+HVGQ MADNPRFGAAIVLPFLT PTSVQVVGTLKPNIHIESLS+ILPFS
Sbjct: 301 SLKLPVVFHNQHVGQFMADNPRFGAAIVLPFLTPPTSVQVVGTLKPNIHIESLSSILPFS 360

Query: 361 ISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV 420
           ISPPF LLPPRS+ VNLS+A+FAGKFSTVSS GSLRLD RKNPIVRFNYLSHPDD++RCV
Sbjct: 361 ISPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVGSLRLDGRKNPIVRFNYLSHPDDVQRCV 420

Query: 421 EGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHY 480
           EGVRKVG+LVNTK MERIKT +L+GK GFEFLG+ LP+NMSDY LVG+FCRKTVTTFWHY
Sbjct: 421 EGVRKVGDLVNTKSMERIKTGDLDGKKGFEFLGAPLPENMSDYRLVGDFCRKTVTTFWHY 480

Query: 481 HGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLG 540
           HGGCLVGKVVD NY+VIGI  LRVVDGSTFSLSPGTNPMATVMMLGRYVGLKML QRLG
Sbjct: 481 HGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQRLG 539

[Close]

BLAST of PI0020304 vs. NCBI nr
Match: XP_008451889.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0055628.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10918.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1042.0 bits (2693), Expect = 1.9e-300
Identity = 526/548 (95.99%), Postives = 535/548 (97.63%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVL-SSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTA 60
           MASLFLLIL L TFQFQIGVL SSQTIPNQDDSYIKF+QNANTLPTTEKYDYIIIGGGTA
Sbjct: 1   MASLFLLILALFTFQFQIGVLISSQTIPNQDDSYIKFIQNANTLPTTEKYDYIIIGGGTA 60

Query: 61  GCPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVEN 120
           GCPLAATLSSNFSVLVLERGSDPNAF MVLSQEGMANTLTDDD+GHNPFQRF+SEDGVEN
Sbjct: 61  GCPLAATLSSNFSVLVLERGSDPNAFPMVLSQEGMANTLTDDDNGHNPFQRFISEDGVEN 120

Query: 121 IRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLA 180
           IRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLA
Sbjct: 121 IRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLA 180

Query: 181 FKEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATV 240
           FKEALVEAGVGPDNGYDL+HV GTRIGGSIFDSRGKRHGA+ELLNKANPINLKVATQATV
Sbjct: 181 FKEALVEAGVGPDNGYDLNHVVGTRIGGSIFDSRGKRHGAVELLNKANPINLKVATQATV 240

Query: 241 KRIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHL 300
           KRIIFSQSNGLSATGVLYSDSNGKLH ATIS NGEIILSAGAIGSPQLLLSSGVGPKSHL
Sbjct: 241 KRIIFSQSNGLSATGVLYSDSNGKLHKATISKNGEIILSAGAIGSPQLLLSSGVGPKSHL 300

Query: 301 SSLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPF 360
           SSLKLPLVLHNRHVGQSMADNPRFGA +VLPFLT PTSVQVVGTLKPNIHIESLSTILPF
Sbjct: 301 SSLKLPLVLHNRHVGQSMADNPRFGATVVLPFLTRPTSVQVVGTLKPNIHIESLSTILPF 360

Query: 361 SISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERC 420
           SISPPFALLPPRSS VNLS+AIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERC
Sbjct: 361 SISPPFALLPPRSSGVNLSLAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERC 420

Query: 421 VEGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWH 480
           VEGVRKVGELVNTKVMERIKTR+LEGKMGFEFLG SLP+NMSDYGLVGEFCRKTVTTFWH
Sbjct: 421 VEGVRKVGELVNTKVMERIKTRDLEGKMGFEFLGRSLPENMSDYGLVGEFCRKTVTTFWH 480

Query: 481 YHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLG 540
           YHGGCLVGKVVD NYKVIGINNLRVVDGSTFSLSPGTNPMA VMMLGRYVGLKMLHQRLG
Sbjct: 481 YHGGCLVGKVVDGNYKVIGINNLRVVDGSTFSLSPGTNPMAAVMMLGRYVGLKMLHQRLG 540

Query: 541 QRSKPFGN 548
           QRSKPFGN
Sbjct: 541 QRSKPFGN 548

[Close]

BLAST of PI0020304 vs. NCBI nr
Match: XP_011648924.1 ((R)-mandelonitrile lyase 1 [Cucumis sativus])

HSP 1 Score: 1037.3 bits (2681), Expect = 4.6e-299
Identity = 522/547 (95.43%), Postives = 533/547 (97.44%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAG 60
           MASLFLLIL L TFQ QI VL+SQTIPNQDDSYIKF+QNANTLPTTEKYDYIIIGGGTAG
Sbjct: 1   MASLFLLILALFTFQLQISVLTSQTIPNQDDSYIKFIQNANTLPTTEKYDYIIIGGGTAG 60

Query: 61  CPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENI 120
           CPLAATLSSNFSVLVLERGSDPNAF MVLSQEGMANTLT+DDDGHNPFQRFVSEDGVENI
Sbjct: 61  CPLAATLSSNFSVLVLERGSDPNAFPMVLSQEGMANTLTEDDDGHNPFQRFVSEDGVENI 120

Query: 121 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAF 180
           RGRVLGGGSMINVGFYSRAQPEFFKNSSVQW+M MVEEAYRWIEETVVSRPELGPWQLAF
Sbjct: 121 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWNMEMVEEAYRWIEETVVSRPELGPWQLAF 180

Query: 181 KEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVK 240
           KEALVEAGVGPDNGYDLDHV GTRIGGSIFDSRGKRHGA+ELLNKANPINLKVATQATVK
Sbjct: 181 KEALVEAGVGPDNGYDLDHVVGTRIGGSIFDSRGKRHGAVELLNKANPINLKVATQATVK 240

Query: 241 RIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLS 300
           RIIFS+SNGLSATGVLYSDSNGKLH ATIS NGEIILSAGAIGSPQLLLSSGVGPKS LS
Sbjct: 241 RIIFSRSNGLSATGVLYSDSNGKLHKATISRNGEIILSAGAIGSPQLLLSSGVGPKSDLS 300

Query: 301 SLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFS 360
           SLKLPLVLHNRHVGQSMADNPR GAAIVLPFLT PTSVQVVGTLKPNIHIESLSTILPFS
Sbjct: 301 SLKLPLVLHNRHVGQSMADNPRLGAAIVLPFLTPPTSVQVVGTLKPNIHIESLSTILPFS 360

Query: 361 ISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV 420
           ISPPFALLPPRSS VNLS+AIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV
Sbjct: 361 ISPPFALLPPRSSAVNLSLAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV 420

Query: 421 EGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHY 480
           EGVRKVGELVNTKVMERIKTR+LEGKMGFEFLGSSLP+NMSDYGLVGEFCRKTVTTFWHY
Sbjct: 421 EGVRKVGELVNTKVMERIKTRDLEGKMGFEFLGSSLPENMSDYGLVGEFCRKTVTTFWHY 480

Query: 481 HGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLGQ 540
           HGGC+VGKVVD NYKVIG+NNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLGQ
Sbjct: 481 HGGCVVGKVVDGNYKVIGVNNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLGQ 540

Query: 541 RSKPFGN 548
           RSKPFGN
Sbjct: 541 RSKPFGN 547

[Close]

BLAST of PI0020304 vs. NCBI nr
Match: XP_038890837.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 983.4 bits (2541), Expect = 7.9e-283
Identity = 495/546 (90.66%), Postives = 518/546 (94.87%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAG 60
           MASLFLLILTL TF  QIGVLSSQTIPNQDDS++KFVQNAN L TTEKYDYIIIGGGTAG
Sbjct: 1   MASLFLLILTLFTFHIQIGVLSSQTIPNQDDSFMKFVQNANALTTTEKYDYIIIGGGTAG 60

Query: 61  CPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENI 120
           CPLAATLSSNFSVLVLERGSDPNAF +VLSQEGMANTLTDDDDG NPFQRFVSEDGVENI
Sbjct: 61  CPLAATLSSNFSVLVLERGSDPNAFPLVLSQEGMANTLTDDDDGSNPFQRFVSEDGVENI 120

Query: 121 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAF 180
           RGRVLGGGSMINVGFYSRAQPEFF+NS VQWDM +VEEAYRWIE+T+VS+PELGPWQ AF
Sbjct: 121 RGRVLGGGSMINVGFYSRAQPEFFQNSGVQWDMKLVEEAYRWIEDTIVSQPELGPWQSAF 180

Query: 181 KEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVK 240
           +EALVEAGVGPDNGYDL HV GTRIGGSIFDSRG+RHGA+ELLNKANP NLKVATQATVK
Sbjct: 181 REALVEAGVGPDNGYDLSHVVGTRIGGSIFDSRGRRHGAVELLNKANPKNLKVATQATVK 240

Query: 241 RIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLS 300
           RIIFSQSNGL+ATGVLYSDS GK+H ATIS NGEIILSAGAIGSPQLLLSSG+GP+SHLS
Sbjct: 241 RIIFSQSNGLTATGVLYSDSKGKVHKATISKNGEIILSAGAIGSPQLLLSSGIGPRSHLS 300

Query: 301 SLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFS 360
           SLKL +VL N HVGQSMADNPRFGAAIVLPFLT PTSVQVVGTLKPNIHIESLSTILPFS
Sbjct: 301 SLKLHVVLDNPHVGQSMADNPRFGAAIVLPFLTPPTSVQVVGTLKPNIHIESLSTILPFS 360

Query: 361 ISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV 420
           ISPPFALLPPRSS VNLSVAIFAGKFSTVSSTGSLRLD  KNPIVRFNYLSHPDDLERCV
Sbjct: 361 ISPPFALLPPRSSAVNLSVAIFAGKFSTVSSTGSLRLDGGKNPIVRFNYLSHPDDLERCV 420

Query: 421 EGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHY 480
           EGVRKVGELVNT+VMERIKTR+LEGKMGF+FLG+SLP+NMSDY LVGEFCRKTVTTFWHY
Sbjct: 421 EGVRKVGELVNTQVMERIKTRDLEGKMGFKFLGTSLPENMSDYCLVGEFCRKTVTTFWHY 480

Query: 481 HGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLGQ 540
           HGGCLVGKVVD NYKVIGI NLRVVDGS FSLSPGTNPMATVMMLGRYVGLKM+ +RLGQ
Sbjct: 481 HGGCLVGKVVDGNYKVIGIKNLRVVDGSIFSLSPGTNPMATVMMLGRYVGLKMMEERLGQ 540

Query: 541 RSKPFG 547
           RSKPFG
Sbjct: 541 RSKPFG 546

[Close]

BLAST of PI0020304 vs. NCBI nr
Match: XP_022929582.1 ((R)-mandelonitrile lyase 1-like [Cucurbita moschata])

HSP 1 Score: 918.7 bits (2373), Expect = 2.4e-263
Identity = 462/539 (85.71%), Postives = 493/539 (91.47%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAG 60
           MA+  LL+L   TFQ QI VL SQTIPNQDDSY+KFVQ ANTLPT E+YDYIIIGGGTAG
Sbjct: 1   MAASLLLLLLFFTFQCQIRVLFSQTIPNQDDSYMKFVQKANTLPTREEYDYIIIGGGTAG 60

Query: 61  CPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENI 120
           CPLAATLSSNFSVLVLERGSDPNAF  VLSQ+G+ANTLTD+DDG NPFQRFVSEDGVENI
Sbjct: 61  CPLAATLSSNFSVLVLERGSDPNAFPSVLSQQGLANTLTDNDDGLNPFQRFVSEDGVENI 120

Query: 121 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAF 180
           RGRVLGGGSMINVGFYSRAQPEFFK+S +QWDM  VE+AY+WIEETVVSRPEL PWQ AF
Sbjct: 121 RGRVLGGGSMINVGFYSRAQPEFFKSSGIQWDMTAVEKAYQWIEETVVSRPELSPWQSAF 180

Query: 181 KEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVK 240
           +E L+EAGVGPDNGYDL H  GTR GGSIFDSRG+RHGA+ELLNKA+P NL+VATQATVK
Sbjct: 181 REVLLEAGVGPDNGYDLKHGVGTRTGGSIFDSRGRRHGAVELLNKADPRNLRVATQATVK 240

Query: 241 RIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLS 300
           RIIFSQSNGLSA+GVLYSD  GKLH ATIS NGEIIL+AGAIGSP LLL SGVGPKSHLS
Sbjct: 241 RIIFSQSNGLSASGVLYSDFKGKLHKATISKNGEIILTAGAIGSPHLLLQSGVGPKSHLS 300

Query: 301 SLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFS 360
           SLKLP+VLHNRHVGQSMADNPRFGAAIVLPFLT PTSVQVVGTLK NIHIESLS+ILPFS
Sbjct: 301 SLKLPVVLHNRHVGQSMADNPRFGAAIVLPFLTPPTSVQVVGTLKRNIHIESLSSILPFS 360

Query: 361 ISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV 420
           I PPF LLPPRS+ VNLS+A+FAGKFSTVSS GSLRLD RKNPIVRFNYLSHPDD+ERCV
Sbjct: 361 IFPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVGSLRLDGRKNPIVRFNYLSHPDDVERCV 420

Query: 421 EGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHY 480
           EGVRKVG+LVNTK MERIKT +LEGK GF+FLGS LP+NMSDY LVG+FCRKTVTTFWHY
Sbjct: 421 EGVRKVGDLVNTKSMERIKTGDLEGKKGFKFLGSPLPENMSDYKLVGDFCRKTVTTFWHY 480

Query: 481 HGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLG 540
           HGGCLVGKVVD NY+VIGI  LRVVDGSTFSLSPGTNPMATVMMLGRYVGLKML QRLG
Sbjct: 481 HGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQRLG 539

[Close]

BLAST of PI0020304 vs. NCBI nr
Match: XP_022997430.1 ((R)-mandelonitrile lyase 1-like [Cucurbita maxima])

HSP 1 Score: 915.2 bits (2364), Expect = 2.6e-262
Identity = 459/539 (85.16%), Postives = 495/539 (91.84%), Query Frame = 0


Query: 1   MASLFLLILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAG 60
           MA+  LL+L L  FQ QI VL SQTIPNQDDSY+KFVQNANTLPT E+YDYIIIGGGTAG
Sbjct: 1   MAASLLLLLLLFIFQCQIPVLFSQTIPNQDDSYMKFVQNANTLPTREEYDYIIIGGGTAG 60

Query: 61  CPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENI 120
           CPLAATLSSNFSVLVLERGSDPNAF  VLSQ+G+ANTLTD+DDG NPFQRFVSEDGVENI
Sbjct: 61  CPLAATLSSNFSVLVLERGSDPNAFPSVLSQQGLANTLTDNDDGLNPFQRFVSEDGVENI 120

Query: 121 RGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAF 180
           RGRVLGGGSMINVGFYSRAQPEFF++S VQWDM  VE+AY+WIEETVVSRPEL PWQ AF
Sbjct: 121 RGRVLGGGSMINVGFYSRAQPEFFQSSGVQWDMMAVEKAYQWIEETVVSRPELSPWQSAF 180

Query: 181 KEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVK 240
           +EAL+EAGVG DNGYDL+HV GTR GGSIFDS G+RHGA+ELLNKA+P NL+VATQATVK
Sbjct: 181 REALLEAGVGRDNGYDLNHVVGTRTGGSIFDSSGRRHGAVELLNKADPRNLRVATQATVK 240

Query: 241 RIIFSQSNGLSATGVLYSDSNGKLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLS 300
           RIIFSQSNGLSA+GVLYSD  GKLH ATIS NGEIIL+AGAIGSP LLL SGVGP+SHLS
Sbjct: 241 RIIFSQSNGLSASGVLYSDFKGKLHKATISRNGEIILTAGAIGSPHLLLQSGVGPRSHLS 300

Query: 301 SLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFS 360
           SLKLP+V HN+HVGQ MADNPRFGAAIVLPFLT PTSVQVVGTLKPNIHIESLS+ILPFS
Sbjct: 301 SLKLPVVFHNQHVGQFMADNPRFGAAIVLPFLTPPTSVQVVGTLKPNIHIESLSSILPFS 360

Query: 361 ISPPFALLPPRSSTVNLSVAIFAGKFSTVSSTGSLRLDRRKNPIVRFNYLSHPDDLERCV 420
           ISPPF LLPPRS+ VNLS+A+FAGKFSTVSS GSLRLD RKNPIVRFNYLSHPDD++RCV
Sbjct: 361 ISPPFGLLPPRSTAVNLSLAVFAGKFSTVSSVGSLRLDGRKNPIVRFNYLSHPDDVQRCV 420

Query: 421 EGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHY 480
           EGVRKVG+LVNTK MERIKT +L+GK GFEFLG+ LP+NMSDY LVG+FCRKTVTTFWHY
Sbjct: 421 EGVRKVGDLVNTKSMERIKTGDLDGKKGFEFLGAPLPENMSDYRLVGDFCRKTVTTFWHY 480

Query: 481 HGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRLG 540
           HGGCLVGKVVD NY+VIGI  LRVVDGSTFSLSPGTNPMATVMMLGRYVGLKML QRLG
Sbjct: 481 HGGCLVGKVVDDNYRVIGIKKLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLQQRLG 539

[Close]

BLAST of PI0020304 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 509.2 bits (1310), Expect = 4.1e-144
Identity = 254/519 (48.94%), Postives = 350/519 (67.44%), Query Frame = 0


Query: 28  NQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFSM 87
           N+   +++F+ NA    + + YDYII+GGGTAGCPLAATLS +F VL+LERG  P     
Sbjct: 33  NRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPN 92

Query: 88  VLSQEGMANTLTDDDDGHNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNS 147
           V+S +G   TLTD ++  +P Q F+SE+GV N RGRVLGG S IN GFYSRA  +FF+NS
Sbjct: 93  VMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENS 152

Query: 148 SVQWDMAMVEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLDHVFGTRIGG 207
            + WD++ V ++Y W+E  +V RP+L  WQ A ++AL+E GV P NG+ L+H  GT+IGG
Sbjct: 153 GLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGG 212

Query: 208 SIFDSRGKRHGALELLNKANPINLKVATQATVKRIIFSQS-----NGLSATGVLYSDSNG 267
           S FD  G+RH + +LL  A   N++VA  ATV+R++ + S     + +SA GV+Y D  G
Sbjct: 213 STFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLG 272

Query: 268 KLHTATISNNGEIILSAGAIGSPQLLLSSGVGPKSHLSSLKLPLVLHNRHVGQSMADNPR 327
           + H A I + GE+ILSAGA+GSPQLL  SG+GP+S+LS+  +P+ L   HVG  + DNPR
Sbjct: 273 RFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPR 332

Query: 328 FGAAIVLPFLTSPTSVQVVGTLKPNIHIESLSTILPFSISPPFALLPPRSSTVNLSVAIF 387
            G +IV P     + +QVVG  +    +E+ S ++PF+       +   +S + + V   
Sbjct: 333 NGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTI 392

Query: 388 AGKFSTVSSTGSLRL---DRRKNPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVMERIK 447
             K     S G LRL   D R NP+VRFNY S P DLERCV G RK+GE++ ++ M+   
Sbjct: 393 MEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFM 452

Query: 448 TRNLEGKMGFEFLGSSLPKNMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDSNYKVIGI 507
            R   G   F F+G+ LP + S+  ++ +FCR+TV+T WHYHGG +VGKVVDS+ KVIG+
Sbjct: 453 IREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGV 512

Query: 508 NNLRVVDGSTFSLSPGTNPMATVMMLGRYVGLKMLHQRL 539
           N+LR+VDGSTF++SPGTNP AT+MMLGRY+GLKML +R+
Sbjct: 513 NSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRERM 551

[Close]

BLAST of PI0020304 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 416.0 bits (1068), Expect = 4.7e-116
Identity = 252/583 (43.22%), Postives = 332/583 (56.95%), Query Frame = 0


Query: 7   LILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTTEKYDYIIIGGGTAGCPLAAT 66
           L + LS F     + SS   PN       F+++A   PTT  YDYIIIGGGTAGCPLAAT
Sbjct: 10  LFVALSLF-LHSPICSSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCPLAAT 69

Query: 67  LSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFVSEDGVENIRGRVLG 126
           LS N SVL+LERG  P     +         L+D  +  +P QRFVSEDGV N R RVLG
Sbjct: 70  LSQNASVLLLERGDSPYNNPNITRLSAFGAALSDLSES-SPSQRFVSEDGVINARARVLG 129

Query: 127 GGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPELGPWQLAFKEALVE 186
           GGS +N GFY+RA  ++ +N  + WD A+  E+Y+W+E  V  +P +G WQ A ++ L+E
Sbjct: 130 GGSALNAGFYTRAGTKYVRN--MGWDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLE 189

Query: 187 AGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLKVATQATVKRIIFSQ 246
           AG+ P+NG+  DH+ GT+ GG+IFD  G RH A +LL  A+P  + V   ATV RI+F +
Sbjct: 190 AGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILF-R 249

Query: 247 SNGLS---ATGVLYSDSNGKLHTATISNN--GEIILSAGAIGSPQLLLSSGVGPKSHLSS 306
           + G +   A GV+Y D  G+ H A +      EIILSAG +GSPQLL+ SGVGP + L +
Sbjct: 250 TRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQA 309

Query: 307 LKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIHIE---------- 366
             + +V+   HVGQ M DNP     +  P     + ++VVG      ++E          
Sbjct: 310 QNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGG 369

Query: 367 ------SLSTILPFSI-SPPFALLPPRSSTVNLSVAIFAGKFSTVS-----STGSLRLDR 426
                 S ST   +++ SP   LL   S T   S   F G F         STG L L  
Sbjct: 370 GGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKT 429

Query: 427 RK---NPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVMERIKTRNLEGKMGFEFL---- 486
           R    NPIV FNY  HPDDL+RCV G++ +  +V +K   R K  +    + FE+L    
Sbjct: 430 RNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYAD----VSFEYLLNLT 489

Query: 487 --------------GSSLPKNMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDSNYKVIG 542
                         G+SLP +        EFC+ TVTT WHYHGGC+VG+VVD +YKVIG
Sbjct: 490 ASTPVNLRPPRSGPGASLPPSAE------EFCQHTVTTIWHYHGGCVVGRVVDGDYKVIG 549

[Close]

BLAST of PI0020304 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 404.8 bits (1039), Expect = 1.1e-112
Identity = 244/589 (41.43%), Postives = 327/589 (55.52%), Query Frame = 0


Query: 4   LFLLILTLSTFQFQIGVLSSQTIPNQDDSYIKFVQNANTLPTT-----------EKYDYI 63
           LF L+L L T          +     +     F+  A+T  ++             YDYI
Sbjct: 7   LFALLLCLPTSLSSTASKGKEKKSKFNPYRYTFIDKASTFSSSSSSSFSSNGQDSSYDYI 66

Query: 64  IIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMANTLTDDDDGHNPFQRFV 123
           +IGGGTAGCPLAATLS NFSVLVLERG  P   + V         L  D    +  Q FV
Sbjct: 67  VIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLA-DISASSASQAFV 126

Query: 124 SEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAMVEEAYRWIEETVVSRPE 183
           S DGV N R RVLGGGS IN GFYSRA   F K +   WD  +V+E+Y W+E  +V +P+
Sbjct: 127 STDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVEREIVHQPK 186

Query: 184 LGPWQLAFKEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGKRHGALELLNKANPINLK 243
           L  WQ A +++L+E GV P NG+  DHV GT+IGG+IFD  G+RH A ELL  ANP  L+
Sbjct: 187 LTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKLR 246

Query: 244 VATQATVKRIIFSQSNGL-SATGVLYSDSNGKLHTATISN--NGEIILSAGAIGSPQLLL 303
           V   ATV++I+F  S      TGV++ D  G  H A +SN    E+ILS+GAIGSPQ+L+
Sbjct: 247 VLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLM 306

Query: 304 SSGVGPKSHLSSLKLPLVLHNRHVGQSMADNPRFGAAIVLPFLTSPTSVQVVGTLKPNIH 363
            SG+GPK  L  LK+P+VL N HVG+ MADNP     +        + +Q VG  K  ++
Sbjct: 307 LSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVGITKMGVY 366

Query: 364 IESLSTI--LPFSISPPFALLPPRSSTVNLSVA---------------------IFAGKF 423
           +E+ +     P SI   + ++  ++   +   A                      F G F
Sbjct: 367 VEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGSF 426

Query: 424 -----STVSSTGSLRLDRRK---NPIVRFNYLSHPDDLERCVEGVRKVGELVNTKVM--- 483
                +   S G L L       NP V FNY  HP DL+RCVE +R V ++V +      
Sbjct: 427 ILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRFLNY 486

Query: 484 ERIKTRNLEGKMGFEFLG--SSLPKNMSDYGLVGEFCRKTVTTFWHYHGGCLVGKVVDSN 543
            +   +N+   +        +  PK ++D   + +FC+ TV T WHYHGGCLVGKVV  N
Sbjct: 487 TQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPN 546

[Close]

BLAST of PI0020304 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-109
Identity = 235/551 (42.65%), Postives = 315/551 (57.17%), Query Frame = 0


Query: 33  YIKFVQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFSMVLSQE 92
           Y  F+++A   P   ++DYIIIGGGT+GC LAATLS N SVLVLERG  P         E
Sbjct: 30  YYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIE 89

Query: 93  GMANTLTDDDDGHNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWD 152
             A TL++     +  Q F+SEDGV N R RVLGGGS++N GFY+RA  E+ K +  +W 
Sbjct: 90  NFATTLSNTSP-KSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKET--EWK 149

Query: 153 MAMVEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLDHVFGTRIGGSIFDS 212
              VE AY W+E+ V  +P +  WQ AFK+ L+EAG  P NG+  DH++GT+IGG+IFD 
Sbjct: 150 TDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDR 209

Query: 213 RGKRHGALELLNKANPINLKVATQATVKRIIFSQSN--GLSATGVLYSDSNGKLHTATIS 272
            G RH A +LL  ANP N+ V   A+V +I+F+        A GV++ D+NG LH A + 
Sbjct: 210 AGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELE 269

Query: 273 NN--GEIILSAGAIGSPQLLLSSGVGPKSHLSSLKL-PLVLHNRHVGQSMADNPRFGAAI 332
            N   E+ILSAGAIGSPQLL+ SG+GP +HL++  + PLVL +  VGQ M DNP     I
Sbjct: 270 KNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFI 329

Query: 333 VLPFLTSPTSVQVVGTLKPNIHIESLSTIL------------------PFSISPPFALLP 392
             P     + +QVVG  K   +IE  S ++                      S   +   
Sbjct: 330 PSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTS 389

Query: 393 PRSSTVNLS---------------VAIFAGKFSTVSSTGSLRLDRRK---NPIVRFNYLS 452
           P  ST +++                 +   K +   S G L L       NP VRFNY  
Sbjct: 390 PTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQ 449

Query: 453 HPDDLERCVEGVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSLPKNMSDYGLVG---- 512
            P+DL+ CVEG+  + +++N+K   + K  +     G   L  S+P N+    +      
Sbjct: 450 EPEDLQICVEGINTIIKVINSKAFSKFKYPDAT-IHGLLDLMLSVPTNLRPRHITSMFNL 509

Query: 513 -EFCRKTVTTFWHYHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLG 538
            +FC  TV T WHYHGGC VG+VVD NY+V+GI++LRV+DGSTF  SPGTNP ATVMMLG
Sbjct: 510 RQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLG 569

[Close]

BLAST of PI0020304 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 393.3 bits (1009), Expect = 3.2e-109
Identity = 239/533 (44.84%), Postives = 312/533 (58.54%), Query Frame = 0


Query: 36  FVQNANTLPTTEKYDYIIIGGGTAGCPLAATLSSNFSVLVLERGSDPNAFSMVLSQEGMA 95
           F+++A   P    +DYIIIGGGTAGC LAATLS N SVLVLERG  P             
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSV 119

Query: 96  NTLTDDDDGHNPFQRFVSEDGVENIRGRVLGGGSMINVGFYSRAQPEFFKNSSVQWDMAM 155
           NTL ++   ++  Q F+SEDGV N R RVLGGGS+IN GFYSRA  ++ + +  +W+M  
Sbjct: 120 NTLLNNTP-NSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEA--EWEMEE 179

Query: 156 VEEAYRWIEETVVSRPELGPWQLAFKEALVEAGVGPDNGYDLDHVFGTRIGGSIFDSRGK 215
           VE AY W+E+ +V  P++  WQ AFK+ L+EAG  PDNG+  DH++GT+IGG+IFD  G 
Sbjct: 180 VEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGH 239

Query: 216 RHGALELLNKANPINLKVATQATVKRIIFSQSNGLSATGVLYSDSNGKLHTATISN--NG 275
           RH A  LL  ANP  + V   A+V +++F+      A  VL+ D+NG  H A ++N    
Sbjct: 240 RHTAANLLEYANPNRIVVYLHASVHKVLFT----TEAYEVLFEDANGVFHKANLANKATN 299

Query: 276 EIILSAGAIGSPQLLLSSGVGPKSHLSSLKL-PLVLHNRHVGQSMADNPRFGAAIVLPFL 335
           E+ILSAGA+GSPQLL+ SGVGP  HL +  + PLVL    VGQ MADNP    AI  P  
Sbjct: 300 EVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQP 359

Query: 336 TSPTSVQVVGTLKPNIHIESLSTI-LPFSISPPF-----ALLPPRSST------------ 395
              + +Q VG  K + +IE LS + L F I+  F      LL   S T            
Sbjct: 360 VELSLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAV 419

Query: 396 ------VNLSVAIFAG----KFSTVSSTGSLRL---DRRKNPIVRFNYLSHPDDLERCVE 455
                 V L V +  G    K    +S G ++L   + R NP V FNY   P+DL +CV+
Sbjct: 420 LLKSFDVKLEVRMNGGLIFQKVDGPASKGHMKLRNTNPRDNPSVTFNYYQEPEDLNKCVK 479

Query: 456 GVRKVGELVNTKVMERIKTRNLEGKMGFEFLGSSL-------PKNMSDYGLVGEFCRKTV 515
           G+  +  ++N+K   + K     G    E L   L       P++++    + +FC  TV
Sbjct: 480 GLNTIIRMINSKAFSKYK---YPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTV 539

Query: 516 TTFWHYHGGCLVGKVVDSNYKVIGINNLRVVDGSTFSLSPGTNPMATVMMLGR 528
           T+ WHYHGGC VGKVVD NYKV+GI+ LRV+DGSTF  SPGTNP ATVMMLGR
Sbjct: 540 TSVWHYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLGR 582

The following BLAST results are available for this feature:

BLAST of PI0020304 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSM2	5.7e-143	48.94	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
P52706	3.7e-142	49.54	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q945K2	5.9e-140	48.98	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
O82784	6.8e-136	48.07	(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1	[more]
P52707	4.8e-134	47.23	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]

BLAST of PI0020304 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UKP1	9.0e-301	95.99	(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3BTC3	9.0e-301	95.99	(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493044 PE=3 SV=...	[more]
A0A0A0LMC2	2.2e-299	95.43	GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G057...	[more]
A0A6J1EN54	1.2e-263	85.71	(R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436125 PE...	[more]
A0A6J1K9L8	1.3e-262	85.16	(R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492348 PE=3...	[more]

BLAST of PI0020304 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008451889.1	1.9e-300	95.99	PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0055628.1 (R)-mand...	[more]
XP_011648924.1	4.6e-299	95.43	(R)-mandelonitrile lyase 1 [Cucumis sativus]	[more]
XP_038890837.1	7.9e-283	90.66	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]
XP_022929582.1	2.4e-263	85.71	(R)-mandelonitrile lyase 1-like [Cucurbita moschata]	[more]
XP_022997430.1	2.6e-262	85.16	(R)-mandelonitrile lyase 1-like [Cucurbita maxima]	[more]

BLAST of PI0020304 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	4.1e-144	48.94	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	4.7e-116	43.22	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	1.1e-112	41.43	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.1	1.1e-109	42.65	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51930.1	3.2e-109	44.84	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.410.40		coord: 169..476 e-value: 1.9E-161 score: 540.3
None	No IPR available	PIRSR	PIRSR000137-2	PIRSR000137-2	coord: 381..536 e-value: 9.9E-36 score: 121.4
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 2..538
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 2..538
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 377..483
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 49..321 e-value: 4.8E-34 score: 118.1
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00624	GMC_OXRED_2	coord: 280..294
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 49..526 e-value: 1.9E-161 score: 540.3
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 44..533
IPR012132	Glucose-methanol-choline oxidoreductase	PIRSF	PIRSF000137	Alcohol_oxidase	coord: 372..543 e-value: 6.8E-28 score: 95.7 coord: 31..340 e-value: 2.3E-56 score: 189.7
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 391..527 e-value: 2.9E-26 score: 92.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0020304.1	PI0020304.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors