PI0019916 (gene) Melon (PI 482460) v1

Overview


Name	PI0019916
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Pectin lyase-like superfamily protein
Location	chr11: 3486398 .. 3488790 (-)
RNA-Seq Expression	PI0019916
Synteny	PI0019916

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTCAATGGGGTTTTCATTGTTATGATTTGGGTTATAGCCATTTTGTTTGTGGAGAATTCAAGTAATGTAAATGGAAGATACCATTTTCATAAGGGTAAAAAGATGAAGATTCCCCTGTTTCTTCACCACCAGAAAATGCAGTTCCTTCAACTCCAGCTCAGCCAGTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAACTGGAAATTCTACTTCTGATTGTGTTTTTGATGTTACTGATTTTGGAGCTGTTGGTGATGGCTGTACTGATGATACTGCTGCCTTTAAGGCTGCATGGAAAGCAGCTTGTGCCGTTGAATCAGCTACTCTTTTAGTTCCTTCTGATCTTTGCTTTAAAATCACGTCAACTATCTTCTCAGGTCCTTGCAAACCTGGACTTGTGTTTAAGGTTAGCAATTCTATTTAAGTTTTGAATTTCTCAAGTAATACTTTCGTGCACGTGCAGACTGGATTACATTATATCCATCTTCATGCATTCCTTCAGTTAACTTTTGTTACGTGTCAACTTGGAATTAGACACTTAAGTGGGATGTAAGAGATAGGTGTAGCTCGAGATGTACTTAAGTACTCTTTCATTTGTAATGAGCGAAAATTCAATCCAAATAGAGACATTTAAGTGTTTTTCTATTTGGAATGAATGGAAGTTCACTCTAGAACAAGTTTTACGTGGAAGTTGTGACTTATGAATGAAAATGAAACGAAACAGGTGGATGGAACTTTGATGCCACCGGATGGACCAGAGTCGTGGCCAAAAGCGGATAGTCCGAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAAGTGGAACCATTGAGGGGAATGGCCAGAAATGGTGGGAGTTGCCATGCAAACCACATAGGGTACCTACATTTGCATTTATTTTCACTCCATCATTTCCGTTGTTATTGATTGTCTCTCCAATTTTCAATCCAAGGCTTAAATTTTCCTTTTTCAGGGGCCAAATGGGTCAACTCTCCCAGGACCTTGTGACAGCCCTGCAGTAAGAAAAAAACGCATCTTTGCTCTTTAATATTTCCCTCTTCATTTACTATATATATGAATCAGAAATGCTGACTGAATATTGCTTTTCTGGTATTTTTCAGTTGATAAGATTCTTCATGAGCAGTAACTTGGCAGTGAATAGTTTGAGAATCCAAAACAGTCCAATGTTCCATATGAAATTTGATGGGTGTGAAGGAGTTCTGATAGAAAAGCTGTCGATTTCTTCCCCAAAGCTCAGTCCCAATACTGATGGAATCCACATAGAGAATACGAAAGGGGTTGGAATATACAACTCCATGATATCCAATGGTATGAAACAATGGTTTAGTCTTGCCTACTGATTTTCAATTGTTATGAAATCGAGAATCAAAGATATTACAGAGATCGACACAAAGATTTACGTGTTTTGCTAACAACGTATTGTCCCGGATAGAAATAAATATCATATTACAAATTTCTAAAACATCAATAGAGTTAGAGAGTTTATATATGATACATCGCTCTAAACCTTAAGACAAAATAAAAAATAACATAAATACAAATACAATTTAAGTTTAATACAAATAACTTATCCACGCATCCCAACACTAATTATCACTTTTTTTTTTTTTTGGCTAATTTGTGATTTGCAAACACAGGTGATGACTGCATTTCAATTGGGCCTGGTTGTGCCAATGTGGCCATTGAAGGGGTTACTTGCGGGCCTAGTCATGGAATTAGGTACCAACAATGCCAATTCAATTTCTCTTGAATGTGAAAATTTCAATCTACAATTATTTGTTTATCACTACTCCAACAGAGAGTAATCCGTTGTTCTATGTTTGATTTTGGGAAAGAGCAGCATTGGGAGCCTAGGAGTCCACAATTCTCAAGCATGTGTTTCCAACATAACAGTAAGAAATGCAGTGATTAGAGATTCAGACAACGGATTGAGGATAAAAACATGGCAAGGAGGGTCGGGATCAGTGAGCGACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATATTACTGCTTGTCAAAGGATTGTCTAAACCAAACATCAGCTGTGTTCGTAAATCAGGTGTTGTACAAGAACATCAAAGGAACTTACGACGTAAGGAACACTCCAATTCACTTCGCTTGCAGTGACTCAGTGGCCTGCACAAATATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAAGGAGAGTTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAAGAGACATTGACCATTCCTCCTATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCTGAGACTTCAGAATATAGCTGCTAA

mRNA sequence

ATGGAATTCAATGGGGTTTTCATTGTTATGATTTGGGTTATAGCCATTTTGTTTGTGGAGAATTCAAGTAATGTAAATGGAAGATACCATTTTCATAAGGAAAATGCAGTTCCTTCAACTCCAGCTCAGCCAGTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAACTGGAAATTCTACTTCTGATTGTGTTTTTGATGTTACTGATTTTGGAGCTGTTGGTGATGGCTGTACTGATGATACTGCTGCCTTTAAGGCTGCATGGAAAGCAGCTTGTGCCGTTGAATCAGCTACTCTTTTAGTTCCTTCTGATCTTTGCTTTAAAATCACGTCAACTATCTTCTCAGGTCCTTGCAAACCTGGACTTGTGTTTAAGGTGGATGGAACTTTGATGCCACCGGATGGACCAGAGTCGTGGCCAAAAGCGGATAGTCCGAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAAGTGGAACCATTGAGGGGAATGGCCAGAAATGGTGGGAGTTGCCATGCAAACCACATAGGGGGCCAAATGGGTCAACTCTCCCAGGACCTTGTGACAGCCCTGCATTGATAAGATTCTTCATGAGCAGTAACTTGGCAGTGAATAGTTTGAGAATCCAAAACAGTCCAATGTTCCATATGAAATTTGATGGGTGTGAAGGAGTTCTGATAGAAAAGCTGTCGATTTCTTCCCCAAAGCTCAGTCCCAATACTGATGGAATCCACATAGAGAATACGAAAGGGGTTGGAATATACAACTCCATGATATCCAATGGTGATGACTGCATTTCAATTGGGCCTGGTTGTGCCAATGTGGCCATTGAAGGGGTTACTTGCGGGCCTAGTCATGGAATTAGCATTGGGAGCCTAGGAGTCCACAATTCTCAAGCATGTGTTTCCAACATAACAGTAAGAAATGCAGTGATTAGAGATTCAGACAACGGATTGAGGATAAAAACATGGCAAGGAGGGTCGGGATCAGTGAGCGACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATATTACTGCTTGTCAAAGGATTGTCTAAACCAAACATCAGCTGTGTTCGTAAATCAGGTGTTGTACAAGAACATCAAAGGAACTTACGACGTAAGGAACACTCCAATTCACTTCGCTTGCAGTGACTCAGTGGCCTGCACAAATATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAAGGAGAGTTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAAGAGACATTGACCATTCCTCCTATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCTGAGACTTCAGAATATAGCTGCTAA

Coding sequence (CDS)

ATGGAATTCAATGGGGTTTTCATTGTTATGATTTGGGTTATAGCCATTTTGTTTGTGGAGAATTCAAGTAATGTAAATGGAAGATACCATTTTCATAAGGAAAATGCAGTTCCTTCAACTCCAGCTCAGCCAGTTCCTTCAGTTCCATCTGATCCTTATCCTAATGATCCTGGAACTGGAAATTCTACTTCTGATTGTGTTTTTGATGTTACTGATTTTGGAGCTGTTGGTGATGGCTGTACTGATGATACTGCTGCCTTTAAGGCTGCATGGAAAGCAGCTTGTGCCGTTGAATCAGCTACTCTTTTAGTTCCTTCTGATCTTTGCTTTAAAATCACGTCAACTATCTTCTCAGGTCCTTGCAAACCTGGACTTGTGTTTAAGGTGGATGGAACTTTGATGCCACCGGATGGACCAGAGTCGTGGCCAAAAGCGGATAGTCCGAGGCAATGGCTTGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAAGTGGAACCATTGAGGGGAATGGCCAGAAATGGTGGGAGTTGCCATGCAAACCACATAGGGGGCCAAATGGGTCAACTCTCCCAGGACCTTGTGACAGCCCTGCATTGATAAGATTCTTCATGAGCAGTAACTTGGCAGTGAATAGTTTGAGAATCCAAAACAGTCCAATGTTCCATATGAAATTTGATGGGTGTGAAGGAGTTCTGATAGAAAAGCTGTCGATTTCTTCCCCAAAGCTCAGTCCCAATACTGATGGAATCCACATAGAGAATACGAAAGGGGTTGGAATATACAACTCCATGATATCCAATGGTGATGACTGCATTTCAATTGGGCCTGGTTGTGCCAATGTGGCCATTGAAGGGGTTACTTGCGGGCCTAGTCATGGAATTAGCATTGGGAGCCTAGGAGTCCACAATTCTCAAGCATGTGTTTCCAACATAACAGTAAGAAATGCAGTGATTAGAGATTCAGACAACGGATTGAGGATAAAAACATGGCAAGGAGGGTCGGGATCAGTGAGCGACATCTTGTTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAATATTACTGCTTGTCAAAGGATTGTCTAAACCAAACATCAGCTGTGTTCGTAAATCAGGTGTTGTACAAGAACATCAAAGGAACTTACGACGTAAGGAACACTCCAATTCACTTCGCTTGCAGTGACTCAGTGGCCTGCACAAATATTACAATGTCTGAAGTAGAGCTGCTGCCACATGAAGGAGAGTTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAAGAGACATTGACCATTCCTCCTATTGATTGCTTGCAAGAGGGAGAACCTCAGAATGTTGCTGAGACTTCAGAATATAGCTGCTAA

Protein sequence

MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHKENAVPSTPAQPVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSDLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC

Homology

[Close]

BLAST of PI0019916 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 2.4e-198
Identity = 334/469 (71.22%), Postives = 382/469 (81.45%), Query Frame = 0


Query: 8   IVMIWVIAILFVENSSNV-NGRYHFHK-----ENAVPSTP------AQPVPSVPSDPYPN 67
           I++I++I+ +   + +NV   RYH+HK      + +P  P      A P   VPSDPYP+
Sbjct: 7   ILVIYIISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPS 66

Query: 68  -DPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSDLCFKITS 127
            DP  G+S S CVFDVT FGAVGDG  DDTAAF+ AWKAACAVES  +L P    FKITS
Sbjct: 67  PDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITS 126

Query: 128 TIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQK 187
           TIFSGPCKPGLVF++DG LMPPDGPE WP+ D+  QWLVFYRLD  T +G GT+EGNGQK
Sbjct: 127 TIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQK 186

Query: 188 WWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLI 247
           WW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V  LRIQNSP FHMKFDGC+GVLI
Sbjct: 187 WWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLI 246

Query: 248 EKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHG 307
            ++ ISSPKLSPNTDGIH+ NT+ VGIYNS++SNGDDCISIG GC++V I+GVTCGPSHG
Sbjct: 247 NEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHG 306

Query: 308 ISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNC 367
           ISIGSLGVHNSQACVSNITVRN VIRDSDNGLR+KTWQGG+GSVS++LFENIQMENV NC
Sbjct: 307 ISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNC 366

Query: 368 IIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVE 427
           IIVDQYYC SKDC N+TSAV V  V Y+NIKGTYDVR+ PIHFACSD+VACTNITMSEVE
Sbjct: 367 IIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVE 426

Query: 428 LLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP-QNVAETSEYSC 463
           LLP EGELV+DPFCWNA+G QETLTIPPIDCL +G P    A  S Y C
Sbjct: 427 LLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

[Close]

BLAST of PI0019916 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 9.2e-86
Identity = 166/388 (42.78%), Postives = 230/388 (59.28%), Query Frame = 0


Query: 64  SDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESAT-LLVPSDLCFKITSTIFSGPCK 123
           S+ +  V +FGA G+G TDDT AF  AWK AC+ +  T +LVP +    +     SGPCK
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 124 PGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKP 183
             L  ++ GT++ P+ P+ W +  + R+WL F+ L ++T+ G GT+ G GQ+WWE  CK 
Sbjct: 109 ARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK- 168

Query: 184 HRGPNGSTLPGPC-DSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISS 243
           H   N      PC  +P  + F    N+ V +L + +S   H+    C  V I  L + +
Sbjct: 169 HNHSN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIA 228

Query: 244 PKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLG 303
           P  SPNTDGIHI  ++G+ I N+ +S GDDCISI      ++I  + CGP HGISIGSLG
Sbjct: 229 PATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLG 288

Query: 304 VHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYY 363
              S   V +ITV  A+I D+ NG+RIKTWQGGSG VS I+F NI+M NV N II+DQYY
Sbjct: 289 KSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYY 348

Query: 364 CLS-KDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEG 423
           C S K C NQTSA+ +  + + +++GT       I  +CSDS  C NI + +++L P  G
Sbjct: 349 CDSRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNG 408

Query: 424 ELVEDPFCWNAFGIQETLTIPPIDCLQE 449
           +   + FCW A+G       PP  CL +
Sbjct: 409 DGFTESFCWEAYGSSSGQVYPP-PCLSD 426

[Close]

BLAST of PI0019916 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 6.0e-85
Identity = 164/357 (45.94%), Postives = 218/357 (61.06%), Query Frame = 0


Query: 67  VFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSDLCFKITSTIFSGPCKPGLV 126
           +F+V  +GAVGDG  D T AF  AW+AAC   SA LLVP +  F + +  F+GPC+P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 127 FKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGP 186
           FKVDG +     P SW    + R WL F +L   TL G G I+G G++WW   CK     
Sbjct: 118 FKVDGIIAAYQNPASW---KNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCK---WV 177

Query: 187 NGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSP 246
           NG  +    D P  I+F  S+ L +  L++ NSP FH+ F  CEGV I  +SI++P+ SP
Sbjct: 178 NGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSP 237

Query: 247 NTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQ 306
           NTDGI I  +K   +  + I  GDDC++IG G +N+ IE + CGP HGISIGSLG  NS+
Sbjct: 238 NTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSR 297

Query: 307 ACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKD 366
           A VS + V  A   D+ NGLRIKTWQGGSG  S I++EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSAS 357

Query: 367 -CLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGEL 423
            C NQ SAV +  V YKNI+GT       I   CSDS+ C +I +S++ L    G++
Sbjct: 358 ACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

[Close]

BLAST of PI0019916 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.5e-83
Identity = 166/350 (47.43%), Postives = 212/350 (60.57%), Query Frame = 0


Query: 67  VFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSDLCFKITSTIFSGPCKPGLV 126
           VF+V  +GAVGDG  D T AF   W AAC   SA LLVP++  F + + +F GPC+P L 
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLS 117

Query: 127 FKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGP 186
           FKVDGT++    P  W  +   + WL F +L    L G+G I+G GQ+WW   CK     
Sbjct: 118 FKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV---V 177

Query: 187 NGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSP 246
           NG T+    + P  I+   S ++ V  L + NSP FH+ F  CEGV I+ L I +P+ SP
Sbjct: 178 NGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSP 237

Query: 247 NTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQ 306
           NTDGI I  +K   I   +I  GDDCI+IG G +N+ I+ + CGP HGISIGSLG  NS+
Sbjct: 238 NTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSR 297

Query: 307 ACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKD 366
           A VS++ V  A   D+ NGLRIKTWQGGSG  S I +EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSAS 357

Query: 367 -CLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVEL 416
            C NQ SAV +  V YKNI GT       I   CSDSV CT I +S V L
Sbjct: 358 ACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSL 400

[Close]

BLAST of PI0019916 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 4.0e-81
Identity = 166/383 (43.34%), Postives = 226/383 (59.01%), Query Frame = 0


Query: 69  DVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSDLCFKITSTIFSGPCKPGLVFK 128
           +V DFGA GDG  DDT AF+ AWKAAC+  S+ +L+     + +    FSGPCK GL  +
Sbjct: 91  NVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSGLTMQ 150

Query: 129 VDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNG 188
           + GT+   D    + K    R WLVF  +  + + G GTI GNG+ WW+  CK ++    
Sbjct: 151 IYGTIEASDDRSDYRK--DGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKAL-- 210

Query: 189 STLPGPC-DSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPN 248
                PC D+P  + F+ S ++ V +L+I+N+   H+ FD C  V    L +++P+ SPN
Sbjct: 211 -----PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPN 270

Query: 249 TDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQA 308
           TDGIH+  T+ + I + +I  GDDCISI  G   V +  +TCGP HGISIGSLG  NS+A
Sbjct: 271 TDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEA 330

Query: 309 CVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYC-LSKD 368
            VS++ V  A +  + NG+RIKTWQGGSGS S+I F+N++M NV N II+DQ YC   K 
Sbjct: 331 HVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKP 390

Query: 369 CLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGELVEDP 428
           C  Q+SAV V  V+Y+NIKGT    N  I F CS    C  I + +V+ L  EG      
Sbjct: 391 CQEQSSAVQVKNVVYQNIKGTC-ASNVAITFDCSKRFPCQGIVLEDVD-LEIEGGAAAKA 450

Query: 429 FCWNAFGIQETLTIPPIDCLQEG 450
            C N   + ET  + P  C +EG
Sbjct: 451 LC-NNVELSETGVVSP-HCQEEG 459

[Close]

BLAST of PI0019916 vs. ExPASy TrEMBL
Match: A0A5D3BVF7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00640 PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.2e-269
Identity = 453/475 (95.37%), Postives = 458/475 (96.42%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK-------------ENAVPSTPAQPVPS 60
           MEFNGV I+MIWVIAILFVENSSNV+GRYHFHK             E AVPSTPAQ VPS
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSD 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPS+
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 463
           ITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

[Close]

BLAST of PI0019916 vs. ExPASy TrEMBL
Match: A0A1S3C7J5 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103497761 PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.2e-269
Identity = 453/475 (95.37%), Postives = 458/475 (96.42%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK-------------ENAVPSTPAQPVPS 60
           MEFNGV I+MIWVIAILFVENSSNV+GRYHFHK             E AVPSTPAQ VPS
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSD 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPS+
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 463
           ITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

[Close]

BLAST of PI0019916 vs. ExPASy TrEMBL
Match: A0A0A0KB54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.2e-259
Identity = 441/475 (92.84%), Postives = 444/475 (93.47%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK-------------ENAVPSTPAQPVPS 60
           ME NGVFIVMIWV+AILFVENSSNVNGRYHFHK             ENAVPSTPAQP+PS
Sbjct: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSTPAQPLPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSD 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPS+
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISN                 GV
Sbjct: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISN-----------------GV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 463
           ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 458

[Close]

BLAST of PI0019916 vs. ExPASy TrEMBL
Match: A0A6J1EWI8 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.5e-251
Identity = 425/484 (87.81%), Postives = 440/484 (90.91%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK----------------------ENAVP 60
           MEFN V  VM+W+ AIL  EN SNV GRYHFHK                      E++VP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPAQPVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           STPA P PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPADPAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSDLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPSD CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETS 463
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

[Close]

BLAST of PI0019916 vs. ExPASy TrEMBL
Match: A0A6J1KH63 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.8e-249
Identity = 421/484 (86.98%), Postives = 438/484 (90.50%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK----------------------ENAVP 60
           MEFN V  VM+W+ AIL  EN SNV GRYHFHK                      E++ P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  STPAQPVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           STPA   PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPADQAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSDLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPSD CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQ+LYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETS 463
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

[Close]

BLAST of PI0019916 vs. NCBI nr
Match: XP_004138524.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN45612.2 hypothetical protein Csa_005406 [Cucumis sativus])

HSP 1 Score: 949.1 bits (2452), Expect = 1.4e-272
Identity = 458/475 (96.42%), Postives = 461/475 (97.05%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK-------------ENAVPSTPAQPVPS 60
           ME NGVFIVMIWV+AILFVENSSNVNGRYHFHK             ENAVPSTPAQP+PS
Sbjct: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKKDEDSSVSSPPENAVPSTPAQPLPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSD 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPS+
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 463
           ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 475

[Close]

BLAST of PI0019916 vs. NCBI nr
Match: XP_008458305.1 (PREDICTED: polygalacturonase At1g48100 [Cucumis melo] >TYK02978.1 polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 938.3 bits (2424), Expect = 2.5e-269
Identity = 453/475 (95.37%), Postives = 458/475 (96.42%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK-------------ENAVPSTPAQPVPS 60
           MEFNGV I+MIWVIAILFVENSSNV+GRYHFHK             E AVPSTPAQ VPS
Sbjct: 1   MEFNGVLILMIWVIAILFVENSSNVDGRYHFHKGKKDEDSPVSSPPEKAVPSTPAQSVPS 60

Query: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSD 120
           VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPS+
Sbjct: 61  VPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSN 120

Query: 121 LCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180
           LCFKITSTIFSGPCKPGLVFKVDGTL PPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT
Sbjct: 121 LCFKITSTIFSGPCKPGLVFKVDGTLTPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGT 180

Query: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240
           IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD
Sbjct: 181 IEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFD 240

Query: 241 GCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300
           GCEGVLIE+LSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV
Sbjct: 241 GCEGVLIERLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGV 300

Query: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360
           TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ
Sbjct: 301 TCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQ 360

Query: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420
           MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN
Sbjct: 361 MENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTN 420

Query: 421 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 463
           ITMSEVELLPHEGELVEDPFCWNAFG+QETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 ITMSEVELLPHEGELVEDPFCWNAFGVQETLTIPPIDCLQEGEPQNVAETSEYSC 475

[Close]

BLAST of PI0019916 vs. NCBI nr
Match: XP_038906648.1 (polygalacturonase At1g48100 [Benincasa hispida])

HSP 1 Score: 919.1 bits (2374), Expect = 1.5e-263
Identity = 443/480 (92.29%), Postives = 455/480 (94.79%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK------------------ENAVPSTPA 60
           MEFNGVFIVMIW++A L VENSSNVNGRYHFHK                  E AVPS+PA
Sbjct: 1   MEFNGVFIVMIWIVATLLVENSSNVNGRYHFHKGKKEEKPNEDSPVFSPPPEKAVPSSPA 60

Query: 61  QPVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATL 120
           +PVPSVPSDPYPN+P  GNS+SDCVFDVTDFGAVGDGC DDTAAFKAAWKAACAVESATL
Sbjct: 61  EPVPSVPSDPYPNEP--GNSSSDCVFDVTDFGAVGDGCADDTAAFKAAWKAACAVESATL 120

Query: 121 LVPSDLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTL 180
           LVPS LCFKITSTIFSGPCKPGLVFKVDGTLMPP+GPESWPKADSPRQWLVFYRLDQMTL
Sbjct: 121 LVPSGLCFKITSTIFSGPCKPGLVFKVDGTLMPPEGPESWPKADSPRQWLVFYRLDQMTL 180

Query: 181 TGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF 240
           TG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF
Sbjct: 181 TGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF 240

Query: 241 HMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV 300
           HMKFDGC+GVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV
Sbjct: 241 HMKFDGCQGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV 300

Query: 301 AIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDIL 360
           AI+GVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNG+RIKTWQGGSGSVSDIL
Sbjct: 301 AIDGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKTWQGGSGSVSDIL 360

Query: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDS 420
           FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSD+
Sbjct: 361 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDT 420

Query: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 463
           VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC
Sbjct: 421 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 478

[Close]

BLAST of PI0019916 vs. NCBI nr
Match: XP_022930280.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 878.2 bits (2268), Expect = 3.0e-251
Identity = 425/484 (87.81%), Postives = 440/484 (90.91%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK----------------------ENAVP 60
           MEFN V  VM+W+ AIL  EN SNV GRYHFHK                      E++VP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPAQPVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           STPA P PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPADPAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSDLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPSD CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETS 463
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

[Close]

BLAST of PI0019916 vs. NCBI nr
Match: XP_023514293.1 (polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 872.5 bits (2253), Expect = 1.7e-249
Identity = 422/484 (87.19%), Postives = 439/484 (90.70%), Query Frame = 0


Query: 1   MEFNGVFIVMIWVIAILFVENSSNVNGRYHFHK----------------------ENAVP 60
           MEFN V  VM+W  AIL  +N SNV GRYHFHK                      +++VP
Sbjct: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60

Query: 61  STPAQPVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120
           STPA P PSVPSDPYP+DP  GN+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPAHPAPSVPSDPYPDDP--GNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120

Query: 121 SATLLVPSDLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLD 180
           SATLLVPSD CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLD 180

Query: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240

Query: 241 SPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPG 300
           SP FHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPG
Sbjct: 241 SPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPG 300

Query: 301 CANVAIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSV 360
           CANV IEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+V
Sbjct: 301 CANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 360

Query: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420
           SDILFENIQMENVRNCIIVDQYYCLSKDC NQTSAVFVNQVLYKNIKGTYDVRNTPIHFA
Sbjct: 361 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 420

Query: 421 CSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETS 463
           CSD+VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET 
Sbjct: 421 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 480

[Close]

BLAST of PI0019916 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 693.0 bits (1787), Expect = 1.7e-199
Identity = 334/469 (71.22%), Postives = 382/469 (81.45%), Query Frame = 0


Query: 8   IVMIWVIAILFVENSSNV-NGRYHFHK-----ENAVPSTP------AQPVPSVPSDPYPN 67
           I++I++I+ +   + +NV   RYH+HK      + +P  P      A P   VPSDPYP+
Sbjct: 7   ILVIYIISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPS 66

Query: 68  -DPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVESATLLVPSDLCFKITS 127
            DP  G+S S CVFDVT FGAVGDG  DDTAAF+ AWKAACAVES  +L P    FKITS
Sbjct: 67  PDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITS 126

Query: 128 TIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQK 187
           TIFSGPCKPGLVF++DG LMPPDGPE WP+ D+  QWLVFYRLD  T +G GT+EGNGQK
Sbjct: 127 TIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQK 186

Query: 188 WWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLI 247
           WW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V  LRIQNSP FHMKFDGC+GVLI
Sbjct: 187 WWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLI 246

Query: 248 EKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHG 307
            ++ ISSPKLSPNTDGIH+ NT+ VGIYNS++SNGDDCISIG GC++V I+GVTCGPSHG
Sbjct: 247 NEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHG 306

Query: 308 ISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNC 367
           ISIGSLGVHNSQACVSNITVRN VIRDSDNGLR+KTWQGG+GSVS++LFENIQMENV NC
Sbjct: 307 ISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNC 366

Query: 368 IIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVE 427
           IIVDQYYC SKDC N+TSAV V  V Y+NIKGTYDVR+ PIHFACSD+VACTNITMSEVE
Sbjct: 367 IIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVE 426

Query: 428 LLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP-QNVAETSEYSC 463
           LLP EGELV+DPFCWNA+G QETLTIPPIDCL +G P    A  S Y C
Sbjct: 427 LLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

[Close]

BLAST of PI0019916 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 540.0 bits (1390), Expect = 1.8e-153
Identity = 257/422 (60.90%), Postives = 321/422 (76.07%), Query Frame = 0


Query: 38  PSTPAQPVPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAV 97
           P  P  P PS+P  P  +DP   N+    +++V  +GAVGDG TDDT AFK AW ++C  
Sbjct: 63  PGPPDSPAPSLPPSP-SDDPADDNNG---IYNVRKYGAVGDGETDDTEAFKTAWDSSCNN 122

Query: 98  ESAT---LLVPSDLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVF 157
           E+ T   LLVP    F I STIF+GPC+    F+VDGT++ PDGPESWP   S RQWLVF
Sbjct: 123 ENNTDSVLLVPYGYTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVF 182

Query: 158 YRLDQMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTL-PGPCDSPALIRFFMSSNLAVNS 217
           YR++ M L G G I+G GQKWW+LPCKPHR  N S +  GPCDSP  +RFFMSSNL V  
Sbjct: 183 YRVNGMALKGEGVIDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEG 242

Query: 218 LRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCI 277
           L+I+NSP FH +FDGC+GV +E L I++P LSPNTDGIHIEN+  V IYNS+ISNGDDC+
Sbjct: 243 LQIKNSPQFHFRFDGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCV 302

Query: 278 SIGPGCANVAIEGVTCGP-SHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQ 337
           SIG G  +V I  +TCGP  HGISIGSLG HNS+ACVSNITVR++VI+ SDNG+RIKTWQ
Sbjct: 303 SIGSGSYDVDIRNLTCGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQ 362

Query: 338 GGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRN 397
           GGSGSVS + F NI +++VRN II+DQYYC++KDC N+TSAVFV+ + Y+ IKGTYD+R+
Sbjct: 363 GGSGSVSGVTFNNIHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRS 422

Query: 398 TPIHFACSDSVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQ 455
            P+HF CSD+V CTN+T+S++ELLP +GE+V DPFCWNA+GI E L+IPP+ CL    P+
Sbjct: 423 PPMHFGCSDAVPCTNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPK 480

[Close]

BLAST of PI0019916 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 513.1 bits (1320), Expect = 2.4e-145
Identity = 242/409 (59.17%), Postives = 304/409 (74.33%), Query Frame = 0


Query: 46  PSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAAC---AVESATL 105
           PS   +P  ND    ++ +  VFDVT FGA+GD  TDDT+AFK AW AAC     +SA L
Sbjct: 29  PSPAPNPAYND---NDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALL 88

Query: 106 LVPSDLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTL 165
           LVP   CF +  T F+GPC+  LV ++DG ++ PDGP SWP ++  RQW++FYR++ +++
Sbjct: 89  LVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWP-SNYQRQWMMFYRVNGLSI 148

Query: 166 TGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMF 225
            GSG I G GQKWW LPCKPH+G NG+T  GPCDSP  IR F SS + +  +   NS  F
Sbjct: 149 QGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQF 208

Query: 226 HMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANV 285
           H++FD C  V+++ + I +P  SPNTDGIHIENT  V I NSMISNGDDCISIG GC NV
Sbjct: 209 HVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNV 268

Query: 286 AIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDIL 345
            I+ VTCGPSHGISIGSLGVHNSQA VSNITV N+ I +SDNG+RIKTWQGGSGSVS I+
Sbjct: 269 DIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIV 328

Query: 346 FENIQMENVRNCIIVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDS 405
           F NI M NVRN I++DQYYC + +C NQTSAV ++ VLY NIKGTYD+R+ PIHF CSDS
Sbjct: 329 FSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDS 388

Query: 406 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEP 452
           V CTN+T++EV+L P +G+ +E+PFCWNA+G  + +T+PP+ CL +  P
Sbjct: 389 VPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

[Close]

BLAST of PI0019916 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 490.7 bits (1262), Expect = 1.3e-138
Identity = 236/448 (52.68%), Postives = 313/448 (69.87%), Query Frame = 0


Query: 6   VFIVMIWVIAILFVENSSNVNGRYHFHKENAVPSTPAQPVPSVPSDPYPNDPGTGNSTSD 65
           +  +   ++ I F   S     R H        S P  P P+ P+DP  +   + +   +
Sbjct: 9   ILCITTLILIITFSLLSYGTEARLHHQ-----ASQPPSPSPN-PNDPSKSPSRSQDLDHE 68

Query: 66  CVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE----SATLLVPSDLCFKITSTIFSGPC 125
            V+DV  +GAVG+G  DDT +FK AW +AC+      ++ L VP    F I STIF+GPC
Sbjct: 69  VVYDVRKYGAVGNGVADDTVSFKTAWDSACSNNKNNTASVLHVPYGFTFMIRSTIFTGPC 128

Query: 126 KPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLDQMTLTGSGTIEGNGQKWWELPCK 185
           +    F+VDGT++P DGP+SWP + + RQWL FYR++ M L G+G I+G GQ WW+LPCK
Sbjct: 129 RSYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCK 188

Query: 186 PH-RGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSIS 245
           PH +  N + L GPC+SPA +RFFMSSN+ V  L I+NSP  H+K DGC  V I  L I 
Sbjct: 189 PHQQNVNKTKLAGPCESPAALRFFMSSNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRII 248

Query: 246 SPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGP-SHGISIGS 305
           SP  SPNTDGIHIEN+  V IYNS+ISNGDDC+SIGPG  ++ I  +TCGP  HGISIGS
Sbjct: 249 SPPASPNTDGIHIENSNSVEIYNSVISNGDDCVSIGPGAYDIDIRNITCGPGGHGISIGS 308

Query: 306 LGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQ 365
           LG  NS ACVSN+TVR++ I+ S+NG+RIKTWQGGSGSVS + F+NI ++ VRN II+DQ
Sbjct: 309 LGEKNSHACVSNVTVRDSFIKFSENGVRIKTWQGGSGSVSGVTFDNIHVDTVRNPIIIDQ 368

Query: 366 YYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHE 425
           YYC +K C N+TSAVFVN ++Y++IKGTYD+R+ P+HF CS++V CTN+T+S +ELLP +
Sbjct: 369 YYCTTKSCANKTSAVFVNDIVYQSIKGTYDIRSPPMHFGCSNNVPCTNLTLSNIELLPSK 428

Query: 426 GELVEDPFCWNAFGIQETLTIPPIDCLQ 448
            ++V  PFCWNA+GI +  ++P I CL+
Sbjct: 429 EDIVVGPFCWNAYGITDEFSVPLISCLK 450

[Close]

BLAST of PI0019916 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.5e-102
Identity = 195/443 (44.02%), Postives = 267/443 (60.27%), Query Frame = 0


Query: 38  PSTPAQPVPSV--PSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAAC 97
           P     P P V  P  P P  P   +      F+V DFGA GDG +DDT AF+AAW +AC
Sbjct: 95  PQVQQPPPPHVQPPPTPLPLQPVEDSQQ----FNVLDFGAKGDGMSDDTQAFEAAWASAC 154

Query: 98  AVESATLLVPSDLCFKITSTIFSGP-CKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVF 157
            VE++T+++P D  F +    FSGP C+  +VF+++G ++ P   ESW        W+ F
Sbjct: 155 KVEASTMIIPPDYIFLVGPISFSGPYCQANIVFQLEGMIVAPTDTESW--GGGLMWWIEF 214

Query: 158 YRLDQMTLTGSGTIEGNGQKWW-------------------------ELPCKPHRGPNGS 217
            +L  +T+ G+G I+G G  WW                         E P  P R     
Sbjct: 215 TKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNW 274

Query: 218 TLPGPCDSPALIRFFMSSNLAVNSLRIQNSPMFHMKFDGCEGVLIEKLSISSPKLSPNTD 277
            +P     P  +RF+ S ++ V  + IQNSP  H+KFD C  VL+  +++SSP  SPNTD
Sbjct: 275 RMPS--IKPTALRFYGSIDVTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTD 334

Query: 278 GIHIENTKGVGIYNSMISNGDDCISIGPGCANVAIEGVTCGPSHGISIGSLGVHNSQACV 337
           GIH++NT+ V I+ + ++ GDDCISI  GC+NV +  V CGP HGISIGSLG  +++ACV
Sbjct: 335 GIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACV 394

Query: 338 SNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKDCLN 397
           SNITVR+ V+ ++  G+RIKTWQGG GSV  ILF NIQ+  V+  I++DQ+YC    C+N
Sbjct: 395 SNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMN 454

Query: 398 QTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVELLPHEGEL-VEDPFC 452
            TSAV V  V Y+ I+GTY V+  P+HFACSDS  C ++ +S +EL P + +  + DPFC
Sbjct: 455 HTSAVSVEGVTYEKIRGTYTVK--PVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFC 514

The following BLAST results are available for this feature:

BLAST of PI0019916 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	2.4e-198	71.22	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
Q94AJ5	9.2e-86	42.78	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P43212	6.0e-85	45.94	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q7M1E7	2.5e-83	47.43	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P35336	4.0e-81	43.34	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]

BLAST of PI0019916 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BVF7	1.2e-269	95.37	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G006...	[more]
A0A1S3C7J5	1.2e-269	95.37	polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103497761 PE=3 SV=1	[more]
A0A0A0KB54	4.2e-259	92.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1	[more]
A0A6J1EWI8	1.5e-251	87.81	polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 P...	[more]
A0A6J1KH63	1.8e-249	86.98	polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=...	[more]

BLAST of PI0019916 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138524.1	1.4e-272	96.42	polygalacturonase At1g48100 [Cucumis sativus] >KGN45612.2 hypothetical protein C...	[more]
XP_008458305.1	2.5e-269	95.37	PREDICTED: polygalacturonase At1g48100 [Cucumis melo] >TYK02978.1 polygalacturon...	[more]
XP_038906648.1	1.5e-263	92.29	polygalacturonase At1g48100 [Benincasa hispida]	[more]
XP_022930280.1	3.0e-251	87.81	polygalacturonase At1g48100-like [Cucurbita moschata]	[more]
XP_023514293.1	1.7e-249	87.19	polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0019916 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48100.1	1.7e-199	71.22	Pectin lyase-like superfamily protein	[more]
AT1G02460.1	1.8e-153	60.90	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	2.4e-145	59.17	Pectin lyase-like superfamily protein	[more]
AT4G01890.1	1.3e-138	52.68	Pectin lyase-like superfamily protein	[more]
AT1G60590.1	1.5e-102	44.02	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 256..277 e-value: 2800.0 score: 4.1 coord: 229..255 e-value: 520.0 score: 9.2 coord: 279..299 e-value: 5300.0 score: 2.1 coord: 309..330 e-value: 6.7 score: 15.7 coord: 338..359 e-value: 390.0 score: 9.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 58..460 e-value: 5.8E-119 score: 399.7
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 99..415 e-value: 3.8E-85 score: 285.8
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 286..299
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..61
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 6..453
None	No IPR available	PANTHER	PTHR31375:SF142	POLYGALACTURONASE PLANT-LIKE PROTEIN	coord: 6..453
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 66..460

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0019916.1	PI0019916.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0004650	polygalacturonase activity