PI0019852 (gene) Melon (PI 482460) v1

BLAST of PI0019852 vs. ExPASy Swiss-Prot
Match: Q9LFN5 (Glutamate receptor 2.5 OS=Arabidopsis thaliana OX=3702 GN=GLR2.5 PE=2 SV=2)

HSP 1 Score: 250.4 bits (638), Expect = 5.5e-65
Identity = 204/712 (28.65%), Postives = 339/712 (47.61%), Query Frame = 0

Query: 12  IHCIASILTHFQCLPKVTIFSQFSNDFSLS-LDSLFDSFLPAGVEVEHHLALSAASNQEI 71
           +  I++I+  F+    V I+    N+F    L +L D+F    V + +  A+S   + + 
Sbjct: 157 VQAISAIIESFRWREVVPIY--VDNEFGEGILPNLVDAFQEINVRIRYRSAISLHYSDD- 216

Query: 72  LIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI------------ 131
            I++EL +LM +   RVFI+  L  +L   L + AK+++M+  GY WI            
Sbjct: 217 QIKKELYKLM-TMPTRVFIVHMLP-DLGSRLFSIAKEIDMLSKGYVWIVTNGIADLMSIM 276

Query: 132 ------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKASIFAIRAYDAA 191
                  M GV+G +TYF  SK+     E +++K +  E        + + FA  AYDAA
Sbjct: 277 GESSLVNMHGVLGVKTYFAKSKE-LLHLEARWQKRFGGE--------ELNNFACWAYDAA 336

Query: 192 RAITRAMEKLGNQNLR---------------------------------SKSNFEGVSGM 251
            A+  ++E++ + N+                                  S  +F+GV+G 
Sbjct: 337 TALAMSVEEIRHVNMSFNTTKEDTSRDDIGTDLDELGVALSGPKLLDALSTVSFKGVAG- 396

Query: 252 VKFSKKNGMLISESPNFKIIKVVDQTYKDVGFWTPNLGEI-------------------- 311
            +F  KNG L  E+  FKII + +   + VGFW   +G +                    
Sbjct: 397 -RFQLKNGKL--EATTFKIINIEESGERTVGFWKSKVGLVKSLRVDKVSHSSRRLRPIIW 456

Query: 312 ----------FDNNHGKKKLNFAVPEEAACKEFVKVS--QHLNGDYITGFSINLFRAVMN 371
                     ++     KKL  AVP++     FV+V+  ++ N   +TGF I++F  VM+
Sbjct: 457 PGDTIFVPKGWEFPTNAKKLRIAVPKKDGFNNFVEVTKDENTNVPTVTGFCIDVFNTVMS 516

Query: 372 NINISDFSCYELVPF-------KGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVS 431
            +  +    YE +PF       +G+YD+M+  V    + GA+GD  ILA+R  YVDF + 
Sbjct: 517 QMPYA--VSYEYIPFDTPDGKPRGSYDEMVYNVFLGEFDGAVGDTTILANRSHYVDFALP 576

Query: 432 YLETEIVMVVKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPE---- 491
           Y ET IV +V  KD K K  W F+     ++W +     L+I  ++W+ E Q + E    
Sbjct: 577 YSETGIVFLVPVKDGKEKGEWVFLKPLTKELWLVTAASFLYIGIMVWIFEYQADEEFREQ 636

Query: 492 --LEGVGNMLWFSISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISW 551
             ++ + ++ +FS S +F+ HR P ++   R+++  W F +L++T S+TA+LTSM+T+  
Sbjct: 637 MIIDKISSVFYFSFSTLFFAHRRPSESFFTRVLVVVWCFVLLILTQSYTATLTSMLTVQE 696

Query: 552 SRPSVPDVKTLKQMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAF----DN 611
            RP+V  +  L++ G N+G  T SF    L   + FD +++K   S +E  + F     N
Sbjct: 697 LRPTVRHMDDLRKSGVNIGYQTGSFTFERL-KQMRFDESRLKTYNSPEEMRELFLHKSSN 756

Query: 612 DTIKAAFFISPHAEVYLAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTET 621
             I AAF    + ++++AKYC  Y+    +FK  G GFA   GS L S +S  I+ +TE 
Sbjct: 757 GGIDAAFDEVAYIKLFMAKYCSEYSIIEPTFKADGFGFAFPLGSPLVSDISRQILNITEG 816

BLAST of PI0019852 vs. ExPASy Swiss-Prot
Match: Q8LGN0 (Glutamate receptor 2.7 OS=Arabidopsis thaliana OX=3702 GN=GLR2.7 PE=2 SV=3)

HSP 1 Score: 233.4 bits (594), Expect = 7.0e-60
Identity = 195/704 (27.70%), Postives = 318/704 (45.17%), Query Frame = 0

Query: 12  IHCIASILTHFQCLPKVTIFSQFSNDFSLS-LDSLFDSFLPAGVEVEHHLALSAASNQEI 71
           +  IA+I+  F     V I+    N+F    L  L D+       V +   +   +N + 
Sbjct: 159 VKAIAAIVKSFGWRNVVAIY--VDNEFGEGILPLLTDALQDVQAFVVNRCLIPQEANDDQ 218

Query: 72  LIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI------------ 131
           ++ +EL +LM  Q  RVF++  +   L      KA+++ M+  GY W+            
Sbjct: 219 IL-KELYKLMTMQ-TRVFVV-HMPPTLGFRFFQKAREIGMMEEGYVWLLTDGVMNLLKSN 278

Query: 132 -------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKASIFAIRAYDA 191
                   M+GV+G  ++   SKK  K+F  +++K+    +P++    + +IFA+RAYD+
Sbjct: 279 ERGSSLENMQGVLGVRSHIPKSKK-LKNFRLRWEKM----FPKKGNDEEMNIFALRAYDS 338

Query: 192 ARAITRAMEKLGNQNLR------------------------------SKSNFEGVSGMVK 251
             A+  A+EK   ++LR                              S   F G++G  +
Sbjct: 339 ITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALSNVRFNGLAG--E 398

Query: 252 FSKKNGMLISESPNFKIIKVVDQTYKDVGFWTPN------------------LGEIFDNN 311
           F   NG L  ES  F +I ++    + +G W P+                  LG +    
Sbjct: 399 FELINGQL--ESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTSVLGERLGPVIWPG 458

Query: 312 HGK------------KKLNFAVPEEAACKEFV--KVSQHLNGDYITGFSINLFRAVMNNI 371
             K            K L   +P +    EFV  K+    N    TG+ I +F AV+  +
Sbjct: 459 KSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEIFEAVLKKL 518

Query: 372 NISDFSCY-ELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVM 431
             S    Y   +     YD+M+  V    Y   +GD+ I+A+R  YVDFT+ Y E+ + M
Sbjct: 519 PYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLPYTESGVSM 578

Query: 432 VVKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEG-----VGNM 491
           +V  KD+  K  W F+  + L +W       +FI F++W++E + N +  G     +G  
Sbjct: 579 MVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGPPHHQIGTS 638

Query: 492 LWFSISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVK 551
            WF+ S + + HRE + + LAR V+  W F +LV+  S+TA+LTS  T+   +P+V + K
Sbjct: 639 FWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLLQPTVTNWK 698

Query: 552 TLKQMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHA 611
            L +   N+G    +F+   L+ +  FD +++K  GS  E  + F N TI A+F    + 
Sbjct: 699 DLIKFNKNIGYQRGTFV-RELLKSQGFDESQLKPFGSAVECDELFSNGTITASFDEVAYI 758

Query: 612 EVYLAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESN-VLA 626
           +V L++    YT    SFK +G GF   K S L   VS +I+ +T+ +E+   E+     
Sbjct: 759 KVILSQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNVTQGEEMQHIENKWFKK 818

BLAST of PI0019852 vs. ExPASy Swiss-Prot
Match: Q9C5V5 (Glutamate receptor 2.8 OS=Arabidopsis thaliana OX=3702 GN=GLR2.8 PE=2 SV=2)

HSP 1 Score: 230.3 bits (586), Expect = 5.9e-59
Identity = 195/703 (27.74%), Postives = 315/703 (44.81%), Query Frame = 0

Query: 9   THPIHCIASILTHFQCLPKVTIFSQFSNDFSLS-LDSLFDSFLPAGVEVEHHLALSAASN 68
           ++ +  IA+I   F     V I+    N+     +  LFD+     V+V+  +  S A++
Sbjct: 150 SYQVKAIAAIFESFGWRSVVAIY--VDNELGEGIMPYLFDAL--QDVQVDRSVIPSEAND 209

Query: 69  QEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWIQ-------- 128
            +IL  +EL +LM  Q  RVF++  ++  L   +  KA ++ M+  GY W+         
Sbjct: 210 DQIL--KELYKLMTRQ-TRVFVV-HMASRLASRIFEKATEIGMMEEGYVWLMTNGMTHMM 269

Query: 129 -----------MEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKASIFAIRA 188
                      ++GV+G  ++   S K  + F  ++K+ +  E P   +    SIF + A
Sbjct: 270 RHIHHGRSLNTIDGVLGVRSHVPKS-KGLEDFRLRWKRNFKKENPWLRD--DLSIFGLWA 329

Query: 189 YDAARAITRAMEKLG-----NQNLRSKSNFEGVSGMVKFSKKNGMLIS------------ 248
           YD+  A+  A+EK         N    SN     G +  S+    L+             
Sbjct: 330 YDSTTALAMAVEKTNISSFPYNNASGSSNNMTDLGTLHVSRYGPSLLEALSEIRFNGLAG 389

Query: 249 ---------ESPNFKIIKVVDQTYKDVGFWTPNLGEIFDNNH----------------GK 308
                    ESP F+II  V    + VGFWTP+ G +  N++                GK
Sbjct: 390 RFNLIDRQLESPKFEIINFVGNEERIVGFWTPSNGLVNVNSNKTTSFTGERFGPLIWPGK 449

Query: 309 --------------KKLNFAVPEEAACKEFVKVSQHLNGDYIT--GFSINLFRAVMNNIN 368
                         KK+   VP +     FV+V      +  T  G++I++F A +  + 
Sbjct: 450 STIVPKGWEIPTNGKKIKVGVPVKKGFFNFVEVITDPITNITTPKGYAIDIFEAALKKLP 509

Query: 369 ISDF-SCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMV 428
            S     Y        YDD++  V N      +GD+ I A+R  Y DFT+ Y E+ + M+
Sbjct: 510 YSVIPQYYRFESPDDDYDDLVYKVDNGTLDAVVGDVTITAYRSLYADFTLPYTESGVSMM 569

Query: 429 VKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEG-----VGNML 488
           V  +D++ K  W F+  + L +W       + I FV+WL E + N +  G     +G   
Sbjct: 570 VPVRDNENKNTWVFLKPWGLDLWVTTACFFVLIGFVVWLFEHRVNTDFRGPPHHQIGTSF 629

Query: 489 WFSISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKT 548
           WFS S + + HRE + + LAR V+  W F +LV+T S+TA+LTS +T+   +P+  +VK 
Sbjct: 630 WFSFSTMVFAHREKVVSNLARFVVVVWCFVVLVLTQSYTANLTSFLTVQRFQPAAINVKD 689

Query: 549 LKQMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAE 608
           L + G  VG    +F+ ++LI    F+ +K+K  GS +E      N +I AAF    +  
Sbjct: 690 LIKNGDYVGYQHGAFVKDFLIKE-GFNVSKLKPFGSSEECHALLSNGSISAAFDEVAYLR 749

Query: 609 VYLAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPF 626
             L++YC  Y     +FK +G GFA  + S L   VS +I+ +T+  E+   E+      
Sbjct: 750 AILSQYCSKYAIVEPTFKTAGFGFAFPRNSPLTGDVSKAILNVTQGDEMQHIENKWFMKQ 809

BLAST of PI0019852 vs. ExPASy Swiss-Prot
Match: O81078 (Glutamate receptor 2.9 OS=Arabidopsis thaliana OX=3702 GN=GLR2.9 PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 5.5e-57
Identity = 199/712 (27.95%), Postives = 326/712 (45.79%), Query Frame = 0

Query: 2   ANIDNNITHPIHCIASILTHFQCLPKVTIF--SQFSNDFSLSLDSLFDSFLPAGVEVEHH 61
           A ID+  +  +  IASI   F+    V I+  ++F   F   +  LFD+     VEV+  
Sbjct: 142 ATIDD--SSQVRAIASIFKFFRWRRVVAIYVDNEFGEGF---MPFLFDAL--QDVEVKRS 201

Query: 62  LALSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWIQ 121
           +    A + E  I++EL +LM  Q  RVF++  +   L   +   A+ + M+  GY W+ 
Sbjct: 202 VIPPEAIDDE--IQKELRKLMERQA-RVFVV-HMESSLALRVFQIARDIGMMEEGYVWLM 261

Query: 122 -------------------MEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTK 181
                              +EGV+G  ++   SK+    F  ++K+ +  E P   +   
Sbjct: 262 TNGMTHMMRHINNGRSLNTIEGVLGVRSHVPKSKE-LGDFRLRWKRTFEKENPSMRD--D 321

Query: 182 ASIFAIRAYDAARAITRAMEK--------------------LGN----------QNLRSK 241
            ++FA+ AYD+  A+ +A+EK                    LGN          Q   S+
Sbjct: 322 LNVFALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAFSE 381

Query: 242 SNFEGVSGMVKFSKKNGMLISESPNFKIIKVVDQTYKDVGFWTPNLGEIFDNNHGK---- 301
             F G++G  +F   +G L  +SP F+II  V    + +GFWTP  G +   +  K    
Sbjct: 382 VRFNGLAG--EFKLIDGQL--QSPKFEIINFVGNEERIIGFWTPRDGLMDATSSNKKTLG 441

Query: 302 -------------------KKLNFAVPEEAACKEFVKVSQH--LNGDYITGFSINLFRAV 361
                              KKL   VP +    +FVKV+ +   N    TG++I +F A 
Sbjct: 442 PVIWPGKSKIVPKGWEIPGKKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIEIFEAA 501

Query: 362 MNNINISDFSCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETE 421
           +  +       Y        Y++++  V +K +   +GDI I A+R  Y DFT+ + E+ 
Sbjct: 502 LKELPYLVIPEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLPFTESG 561

Query: 422 IVMVVKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEG-----V 481
           + M+V  +D++ K  W F+  + L++W       +FI FV+WL E + N +  G     +
Sbjct: 562 VSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGPPQYQI 621

Query: 482 GNMLWFSISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVP 541
           G  LWFS S + + HRE + + LAR V+  W F +LV+T S+TASLTS +T+   +P+V 
Sbjct: 622 GTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSLQPTVT 681

Query: 542 DVKTLKQMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGS---IDEYPKAFDNDTIKAAF 601
           +V  L +    VG    +F+ + L+  L F   ++K   S    D+      +  I AAF
Sbjct: 682 NVNDLIKNRDCVGYQGGAFVKDILLG-LGFHEDQLKPFDSAKDADDLLSKGKSKGIAAAF 741

Query: 602 FISPHAEVYLAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFE 622
               + +  L++ C  Y     +FK  G GFA  K S L    S +I+ LT+     Q E
Sbjct: 742 DEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNVTQQIE 801

BLAST of PI0019852 vs. ExPASy Swiss-Prot
Match: Q93YT1 (Glutamate receptor 3.2 OS=Arabidopsis thaliana OX=3702 GN=GLR3.2 PE=1 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 2.6e-54
Identity = 174/629 (27.66%), Postives = 283/629 (44.99%), Query Frame = 0

Query: 101 LLTKAKKLNMVGNGYTWI---------------------QMEGVIGFETYFNDSKKSFKS 160
           +  +A+KL M+  GY WI                      + GV+    +  +SKK  K 
Sbjct: 238 IFEEAQKLGMMEKGYVWIATTWLTSLLDSVNPLPAKTAESLRGVLTLRIHTPNSKKK-KD 297

Query: 161 FETKFKKIYNLEYPQEEEPTKASIFAIRAYDAARAITRAMEKL----------------- 220
           F  ++ K+ N            +++ + AYD    I RA+++L                 
Sbjct: 298 FVARWNKLSN-------GTVGLNVYGLYAYDTVWIIARAVKRLLDSRANISFSSDPKLTS 357

Query: 221 ----GNQNLRSKS---------------NFEGVSGMVKFSKKNGMLISESPNFKIIKVVD 280
               G+ NL + S               N  GV+G ++F     M+    P++ II VVD
Sbjct: 358 MKGGGSLNLGALSIFDQGSQFLDYIVNTNMTGVTGQIQFLPDRSMI---QPSYDIINVVD 417

Query: 281 QTYKDVGFW---------------------------------------TPNLGEIFDNNH 340
             ++ +G+W                                       TP  G +F NN 
Sbjct: 418 DGFRQIGYWSNHSGLSIIPPESLYKKLSNRSSSNQHLNNVTWPGGTSETPR-GWVFPNN- 477

Query: 341 GKKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNINISDFSCYELVPF-- 400
             ++L   VP+ A+ KEF  VS+    + + G++I++F A +  I+      +E V F  
Sbjct: 478 -GRRLRIGVPDRASFKEF--VSRLDGSNKVQGYAIDVFEAAVKLISYP--VPHEFVLFGD 537

Query: 401 ---KGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMV--VKQKDDKW 460
                 +++ +  V+  ++   +GDI I+  R + VDFT  Y+E+ +V+V  V + +D  
Sbjct: 538 GLKNPNFNEFVNNVTIGVFDAVVGDIAIVTKRTRIVDFTQPYIESGLVVVAPVTKLND-- 597

Query: 461 KKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEG-----VGNMLWFSISIIF 520
              WAF+  F   MW +     L +  VIW++E + N E  G     +  +LWFS S +F
Sbjct: 598 -TPWAFLRPFTPPMWAVTAAFFLIVGSVIWILEHRINDEFRGPPRKQIVTILWFSFSTMF 657

Query: 521 YMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMGYNV 580
           + HRE   + L R VL  WLF +L++T+S+TASLTS++T+      +  V TL      V
Sbjct: 658 FSHRENTVSTLGRAVLLIWLFVVLIITSSYTASLTSILTVQQLNSPIRGVDTLISSSGRV 717

Query: 581 GCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAKYCK 618
           G    S+  NY+ID LN   +++  +GS  EY  A  N T+ A     P+ +++L+++C 
Sbjct: 718 GFQVGSYAENYMIDELNIARSRLVPLGSPKEYAAALQNGTVAAIVDERPYVDLFLSEFC- 777

BLAST of PI0019852 vs. ExPASy TrEMBL
Match: A0A5A7V226 (Glutamate receptor 2.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00400 PE=3 SV=1)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 571/704 (81.11%), Postives = 596/704 (84.66%), Query Frame = 0

Query: 1   MANIDNNITHPIHCIASILTHFQCLPKVTIFSQFSNDFSLS--LDSLFDSFLPAGVEVEH 60
           MAN+DNNITHPIHCIASILTHFQCLP+VTIF Q +ND SLS     LF SFL AGVEVEH
Sbjct: 1   MANVDNNITHPIHCIASILTHFQCLPEVTIFYQSTNDPSLSHLHHRLFGSFLLAGVEVEH 60

Query: 61  HLALSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI 120
           HLALS A+NQE LIEQELTRLMN+QRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI
Sbjct: 61  HLALSPATNQETLIEQELTRLMNNQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI 120

Query: 121 --------------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEP 180
                               +MEGVIG ETYFNDSKKSFKSFETKFKKIY LEYPQ+EEP
Sbjct: 121 ISHEVFDLISSLDSSSSLLSKMEGVIGLETYFNDSKKSFKSFETKFKKIYKLEYPQDEEP 180

Query: 181 TKASIFAIRAYDAARAITRAMEKLG-NQNLRS----------KSNFEGVSGMVKFSKKNG 240
           TKASIFAIRAYDAARA+TRAMEKLG ++ LRS          +SNFEGV GMVKFSK+NG
Sbjct: 181 TKASIFAIRAYDAARAVTRAMEKLGDDKKLRSSSKQLREKILESNFEGVGGMVKFSKQNG 240

Query: 241 MLISESPNFKIIKVVDQTYKDVGFWTPNLG------------------------------ 300
           MLISESPNFKI+KVVDQTYK+VGFWTPNLG                              
Sbjct: 241 MLISESPNFKIVKVVDQTYKEVGFWTPNLGFVENYVEIINKTTTELVKHNKGNLRKIVSV 300

Query: 301 -----------EIFDNNHGKKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVM 360
                      E FDN+HGKK+L FAVPEEAACKE VKVSQHLNGDYITGFSINLFRAVM
Sbjct: 301 GNLSRLKTSSSENFDNDHGKKRLTFAVPEEAACKELVKVSQHLNGDYITGFSINLFRAVM 360

Query: 361 NNINISDFSCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEI 420
           NNIN SDFS YELVPFKG YDDM+KAVSNK Y GA+GDI ILAHRYKYVDFTVSYL+TEI
Sbjct: 361 NNINTSDFSSYELVPFKGRYDDMIKAVSNKTYFGAVGDIIILAHRYKYVDFTVSYLQTEI 420

Query: 421 VMVVKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWF 480
           VMVV+QKDDKWKKIWAFM AF+L MW LIPTMHLFISFVIWLIERQNNPELEGVGNMLWF
Sbjct: 421 VMVVEQKDDKWKKIWAFMEAFELTMWLLIPTMHLFISFVIWLIERQNNPELEGVGNMLWF 480

Query: 481 SISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLK 540
           SISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISW RPSVPDV+TLK
Sbjct: 481 SISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWFRPSVPDVETLK 540

Query: 541 QMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVY 600
           QMGYNVGCNTNSFICNYL+DTL FDPTKIKKI SIDEYPKAF N TIKAAFFISPHA+VY
Sbjct: 541 QMGYNVGCNTNSFICNYLVDTLEFDPTKIKKIDSIDEYPKAFKNGTIKAAFFISPHAKVY 600

Query: 601 LAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNC 631
           LAKYCKGYTKGVSSFKLSGIGFAM KGSELASRVSASIVELTETKEIPQFES+VLA FNC
Sbjct: 601 LAKYCKGYTKGVSSFKLSGIGFAMAKGSELASRVSASIVELTETKEIPQFESDVLASFNC 660

BLAST of PI0019852 vs. ExPASy TrEMBL
Match: A0A0A0LNF4 (PBPe domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G357220 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.3e-303
Identity = 544/703 (77.38%), Postives = 589/703 (83.78%), Query Frame = 0

Query: 1   MANIDNNITHPIHCIASILTHFQCLPKVTIFSQFSNDFSLSLDSLFDSFLPAGVEVEHHL 60
           MAN DN+IT+PIHCIASIL+HFQCLPKVTIF Q +ND SLSL   FDSFLPAGVEVEH L
Sbjct: 1   MANTDNDITYPIHCIASILSHFQCLPKVTIFYQITNDPSLSLHRFFDSFLPAGVEVEHRL 60

Query: 61  ALSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI-- 120
           ALS+ASNQEI+IEQELTRLMN+QR+R FIITQLSLELVDLLLTKAKKLNMVGNGYTWI  
Sbjct: 61  ALSSASNQEIVIEQELTRLMNNQRSRNFIITQLSLELVDLLLTKAKKLNMVGNGYTWIIS 120

Query: 121 ------------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTK 180
                             +MEGVIGF TYFNDS+KSFKSFETKFKKIY LEYPQEEEPTK
Sbjct: 121 HEVFDLISYLDSSSSLLSKMEGVIGFGTYFNDSRKSFKSFETKFKKIYRLEYPQEEEPTK 180

Query: 181 ASIFAIRAYDAARAITRAMEKLGNQNLRS----------KSNFEGVSGMVKFSKKNGMLI 240
           ASIFAIRAYDAAR I RAME+LG++NLRS          +SNFEGVSGMVKFSKKNGMLI
Sbjct: 181 ASIFAIRAYDAARNIIRAMERLGDENLRSSSKQLMDKILESNFEGVSGMVKFSKKNGMLI 240

Query: 241 SESPNFKIIKVVDQTYKDVGFWTPNLG--------------------------------- 300
           SESPNFKI+KVVDQTYK+VGFWTPNLG                                 
Sbjct: 241 SESPNFKIVKVVDQTYKEVGFWTPNLGFVENYVEIISKTTTKLVKHSKGNLRKNLSVGDL 300

Query: 301 --------EIFDNNHGKKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNI 360
                   E FDN+H KKK  FAVPE+AACKEFVKVSQHLNG+YITGF++ LFRAVMNNI
Sbjct: 301 SRPKTSSSENFDNHHSKKKFKFAVPEDAACKEFVKVSQHLNGNYITGFAVTLFRAVMNNI 360

Query: 361 NISDFSCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMV 420
           N+S+FS YELVP KGTY+ M++ VS KI+ GA+GDIGILA RYK+VD+TVSYLETEIVMV
Sbjct: 361 NMSEFSDYELVPLKGTYNKMIEDVSKKIFFGAVGDIGILAQRYKHVDYTVSYLETEIVMV 420

Query: 421 VKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSIS 480
           V+QKDDKWKKIWAFMGAFQL MW LIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSIS
Sbjct: 421 VQQKDDKWKKIWAFMGAFQLTMWLLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSIS 480

Query: 481 IIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMG 540
           I+FYMHREP+KNG+ARLVLGPWLFAILV+TASFTASL SMMT SW RPSVPDV+TL++MG
Sbjct: 481 IVFYMHREPVKNGMARLVLGPWLFAILVITASFTASLASMMTNSWLRPSVPDVETLRKMG 540

Query: 541 YNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAK 600
           +NVGCNTNSFIC+YL DTL FDP KIKKI  +DEYPKAF++ TIKAAFFISPHA VYLAK
Sbjct: 541 HNVGCNTNSFICSYLADTLKFDPEKIKKIDLVDEYPKAFESGTIKAAFFISPHARVYLAK 600

Query: 601 YCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNCSSN 633
            CKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTET EIPQFESNVLA FNCSSN
Sbjct: 601 NCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETNEIPQFESNVLASFNCSSN 660

BLAST of PI0019852 vs. ExPASy TrEMBL
Match: A0A6J1K353 (glutamate receptor 2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111491966 PE=3 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 9.3e-233
Identity = 435/682 (63.78%), Postives = 514/682 (75.37%), Query Frame = 0

Query: 8   ITHPIHCIASILTHFQCLPKVTIFSQFSND------FSLSLDSLFDSFLPAGVEVEHHLA 67
           ITH I CIASILTHFQ   KVTIF   SN        ++S + LFDS   A VEVEH LA
Sbjct: 163 ITHRIQCIASILTHFQ-WRKVTIFYDISNTDHSPIYSAVSANRLFDSLRLADVEVEHRLA 222

Query: 68  LSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI--- 127
           LS++SNQEILIEQEL +LMNSQRNRVF++TQL +EL  L+L +AKKLNMVGNGY WI   
Sbjct: 223 LSSSSNQEILIEQELKKLMNSQRNRVFVVTQLEVELAVLVLEQAKKLNMVGNGYVWIVSN 282

Query: 128 ---------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKASI 187
                          +MEGVIGF TYF+D+K SFKSFETKFKK+Y LEYPQEEEPT+ASI
Sbjct: 283 DVFDAIDSLDSSFWYKMEGVIGFRTYFDDTKNSFKSFETKFKKMYRLEYPQEEEPTRASI 342

Query: 188 FAIRAYDAARAITRAM-EKLGNQNLRSK---SNFEGVSGMVKFSKKNGMLISESPNFKII 247
             +RAYDAARAITRAM E L    +  K   SNFEG+SGMV+F  KNGMLIS+SPNFKI 
Sbjct: 343 SVVRAYDAARAITRAMGENLSWSEVLEKIGESNFEGLSGMVRF--KNGMLISQSPNFKIS 402

Query: 248 KVVDQTYKDVGFWTPNLGEI----------------------------------FDNNHG 307
           KVV Q++K+VGFWTP LG +                                   +N  G
Sbjct: 403 KVVGQSFKEVGFWTPKLGFVERFVEVNKTTTKLKPKFGNLGNVAVGDLPRLTTSSENCDG 462

Query: 308 KKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNINISDFSCYELVPFKGT 367
           +K+L FAVPEE AC+E VKVS+HL G Y+TGFSIN+FRAVM+NINIS    Y+L+PFKG 
Sbjct: 463 EKRLRFAVPEEGACQEIVKVSKHLEGYYVTGFSINVFRAVMSNINISHPLSYDLLPFKGK 522

Query: 368 YDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMVVKQKDDKWKKIWAFMG 427
           Y+DM++AV NK Y GA+G+IGIL +R+  VDFTVSYLETEIVMVVK+K  +WK++WAF  
Sbjct: 523 YEDMLEAVRNKTYDGAVGEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSRWKQLWAFTD 582

Query: 428 AFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSISIIFYMHREPLKNGLAR 487
           AF++ MW LIPTMHLFISF +WLIERQN+ EL+G GNMLWFS+SIIFYMHREP+KNGLAR
Sbjct: 583 AFEVSMWLLIPTMHLFISFAVWLIERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGLAR 642

Query: 488 LVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMGYNVGCNTNSFICNYLI 547
           LVLGPWLF ILVVTASFT+SLTSMMT+SW RPSV DV  LK++   VGCN  SFIC+YL 
Sbjct: 643 LVLGPWLFGILVVTASFTSSLTSMMTVSWYRPSVLDVVKLKEINAVVGCNARSFICDYLK 702

Query: 548 DTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAKYCKGYTKGVSSFKLSG 607
            TL F+P+KIK+I S++ YPKAF+++TIKAAFFISPHA+V+LAK C+GYTKGVSS+KLSG
Sbjct: 703 KTLKFEPSKIKRIDSLNNYPKAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSYKLSG 762

Query: 608 IGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNCSSNGKGHGLGLGPEPFMGL 628
           +GFA  KGS LA++VSASIVELTETKE+PQF+ N L  FNC +  +       P PFMGL
Sbjct: 763 VGFAFAKGSPLAAKVSASIVELTETKEMPQFDPNYLVSFNCPTAKE-------PGPFMGL 822

BLAST of PI0019852 vs. ExPASy TrEMBL
Match: A0A6J1HC86 (glutamate receptor 2.5-like OS=Cucurbita moschata OX=3662 GN=LOC111462764 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 6.7e-231
Identity = 433/684 (63.30%), Postives = 512/684 (74.85%), Query Frame = 0

Query: 6   NNITHPIHCIASILTHFQCLPKVTIFSQFSN------DFSLSLDSLFDSFLPAGVEVEHH 65
           N+ITH I CI SILTHFQ   KVTIF + SN        ++S + LFDS   A VEVEH 
Sbjct: 159 NHITHRIQCIVSILTHFQ-WRKVTIFYEISNIDHFPISSAVSANRLFDSLRLADVEVEHR 218

Query: 66  LALSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI- 125
           LALS++SNQEILIEQEL RL NSQRNRVF++TQL +EL  L+L KAKKLNMVGNGY WI 
Sbjct: 219 LALSSSSNQEILIEQELKRLKNSQRNRVFVVTQLEVELAVLVLEKAKKLNMVGNGYVWIV 278

Query: 126 -----------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKA 185
                            +M+GVIGF TYFND+K SFKSFETKFKK+Y LEYPQEEEPT+A
Sbjct: 279 SNDVFDLMDSLDSSFWYKMDGVIGFGTYFNDTKNSFKSFETKFKKMYRLEYPQEEEPTRA 338

Query: 186 SIFAIRAYDAARAITRAM-EKLGNQNLRSK---SNFEGVSGMVKFSKKNGMLISESPNFK 245
           SI  +RAYDAARA+TRAM E L +  +  K   SNFEG+SG V+F  KNG LIS+SPNFK
Sbjct: 339 SISVVRAYDAARAMTRAMGENLISSEVLEKIGESNFEGLSGRVRF--KNGKLISQSPNFK 398

Query: 246 IIKVVDQTYKDVGFWTPNLG--EIF--------------------------------DNN 305
           I KVVDQ++K+V FWTP LG  E F                                +N 
Sbjct: 399 ISKVVDQSFKEVAFWTPKLGFFERFVEVNKTTTKLKPKFGNLGNVAVGDLPRLTTSSENC 458

Query: 306 HGKKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNINISDFSCYELVPFK 365
            G+K+L FAVPEE AC+E VKVS+HL G Y+TGFSIN+FRAVM+NINIS    Y+L+PFK
Sbjct: 459 EGEKRLRFAVPEEGACQEIVKVSKHLEGYYVTGFSINVFRAVMSNINISHPLSYDLLPFK 518

Query: 366 GTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMVVKQKDDKWKKIWAF 425
           G Y+DM++AV NK Y GA+G+IGIL +R+  VDFTVSYLETEIVMVVK+K  +WK++WAF
Sbjct: 519 GKYEDMLEAVRNKTYDGAVGEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSQWKQLWAF 578

Query: 426 MGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSISIIFYMHREPLKNGL 485
             AF +  W LIPTMHLFISF +WL+ERQN+ EL+G GNMLWFS+SIIFYMHREP+KNGL
Sbjct: 579 TEAFDVSTWLLIPTMHLFISFSVWLLERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGL 638

Query: 486 ARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMGYNVGCNTNSFICNY 545
           ARLVLGPWLF ILVVTASFT+SLTSMMT++W RPSV DV  LK+M   VGCN  SFIC+Y
Sbjct: 639 ARLVLGPWLFGILVVTASFTSSLTSMMTVTWYRPSVLDVAKLKEMNAVVGCNARSFICDY 698

Query: 546 LIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAKYCKGYTKGVSSFKL 605
           L  TL F+P+KIK+I S++ YPKAF++D+IKAAFFISPHA+V+LAK C+GYTKGVSS+KL
Sbjct: 699 LEKTLKFEPSKIKRIASLNNYPKAFEDDSIKAAFFISPHADVFLAKNCRGYTKGVSSYKL 758

Query: 606 SGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNCSSNGKGHGLGLGPEPFM 628
           SG+GFA  KGS LA++VSASIVELTETKE+PQF+ N L  FNC +          P PFM
Sbjct: 759 SGVGFAFAKGSPLAAKVSASIVELTETKEMPQFDPNYLVSFNCPT-------AKAPGPFM 818

BLAST of PI0019852 vs. ExPASy TrEMBL
Match: A0A6J1K7S1 (glutamate receptor 2.5-like OS=Cucurbita maxima OX=3661 GN=LOC111492435 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 3.3e-222
Identity = 417/690 (60.43%), Postives = 500/690 (72.46%), Query Frame = 0

Query: 6   NNITHPIHCIASILTHFQCLPKVTIFSQFSNDFSLSLDSLFDSFLPAGVEVEHHLALSAA 65
           NNITH I CIASIL +FQ  PKV+IF  +   F+ S+  LFDSF  A +EVE+H ALS++
Sbjct: 157 NNITHHIKCIASILKYFQKPPKVSIF--YDPTFN-SVHRLFDSFQLANIEVENHFALSSS 216

Query: 66  SNQ-EILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI------ 125
           SNQ EI IE EL R+M SQRN VF++TQLSLELV+LL  KAKK+NMVGNGYTWI      
Sbjct: 217 SNQDEISIENELKRVMKSQRNGVFVVTQLSLELVNLLFIKAKKMNMVGNGYTWIVSNDVF 276

Query: 126 ----------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKAS 185
                           +MEGVIGF TYF+D+K  FK FETKFKK+Y LEYP++EEP KAS
Sbjct: 277 DLIDSSLINDHSPLLNKMEGVIGFRTYFDDTKMPFKRFETKFKKMYTLEYPEDEEPIKAS 336

Query: 186 IFAIRAYDAARAITRAMEKLGNQNLRS---------KSNFEGVSGMVKFSKKNGMLISES 245
            FA+RAYDA R I RAME LG+ NLRS         +SNFEGVSGMV+F  K+GMLI +S
Sbjct: 337 SFAVRAYDAVRCIARAMETLGDNNLRSSDQVLKTILESNFEGVSGMVRF--KDGMLIPQS 396

Query: 246 PNFKIIKVVDQTYKDVGFWTPNLG------EIFDN--------NHGKK------------ 305
           PNFKIIKVVDQ YKDV FW+P LG      E+ DN        N G+             
Sbjct: 397 PNFKIIKVVDQRYKDVAFWSPKLGFSESFLEVEDNKAAIKVKPNMGRHVDGGDLSRKAIT 456

Query: 306 ----------KLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNINISDFSCY 365
                     KL FAVP + AC+E VKVSQHLNG+Y TG SI++F+A M NIN+   S Y
Sbjct: 457 LWSQSSGEEGKLKFAVPRKGACQELVKVSQHLNGNYFTGLSIDVFKAAMTNINMPYSSSY 516

Query: 366 ELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMVVKQKDDKW 425
           +L PF GTY++MMKAV NK Y GA+GDI I+A R++ VDF+V+YLE +IVMVVK++ DKW
Sbjct: 517 DLYPFDGTYEEMMKAVHNKTYDGAVGDISIMAQRFESVDFSVAYLEADIVMVVKEEQDKW 576

Query: 426 KKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSISIIFYMHRE 485
           K +W F  AF++ +W LIPT+HLFIS VIW+IER+NN EL+G+G+MLWFS+S+I Y  RE
Sbjct: 577 KSLWVFFEAFEVTVWLLIPTIHLFISSVIWVIERRNNEELKGLGSMLWFSVSLISYTQRE 636

Query: 486 PLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMGYNVGCNTN 545
           P+KNGL+RLVLGPWLF ILVVT SF+ASLTSMMT+SWS+P +PDVKTLK+M  +VGCNT 
Sbjct: 637 PVKNGLSRLVLGPWLFGILVVTESFSASLTSMMTVSWSQPLIPDVKTLKKMDASVGCNTE 696

Query: 546 SFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAKYCKGYTKG 605
           SFICNYL  +L F+  KIKK+ +ID+YPKA DN +IKAAF I PHA+V+LAKYC GYTK 
Sbjct: 697 SFICNYLNGSLKFENAKIKKMNTIDDYPKALDNGSIKAAFLIRPHADVFLAKYCIGYTKT 756

Query: 606 VSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNCSSNGKGHGLGL 628
             S KL GIGFA  KGS L   VSASIVEL ET +IPQ E  +LA FNCSS G+G GL L
Sbjct: 757 NFSIKLGGIGFAFRKGSALGVDVSASIVELIETNDIPQMERTLLASFNCSSTGQGDGLRL 816

BLAST of PI0019852 vs. NCBI nr
Match: KAA0060907.1 (glutamate receptor 2.5-like [Cucumis melo var. makuwa])

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 571/704 (81.11%), Postives = 596/704 (84.66%), Query Frame = 0

Query: 1   MANIDNNITHPIHCIASILTHFQCLPKVTIFSQFSNDFSLS--LDSLFDSFLPAGVEVEH 60
           MAN+DNNITHPIHCIASILTHFQCLP+VTIF Q +ND SLS     LF SFL AGVEVEH
Sbjct: 1   MANVDNNITHPIHCIASILTHFQCLPEVTIFYQSTNDPSLSHLHHRLFGSFLLAGVEVEH 60

Query: 61  HLALSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI 120
           HLALS A+NQE LIEQELTRLMN+QRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI
Sbjct: 61  HLALSPATNQETLIEQELTRLMNNQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI 120

Query: 121 --------------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEP 180
                               +MEGVIG ETYFNDSKKSFKSFETKFKKIY LEYPQ+EEP
Sbjct: 121 ISHEVFDLISSLDSSSSLLSKMEGVIGLETYFNDSKKSFKSFETKFKKIYKLEYPQDEEP 180

Query: 181 TKASIFAIRAYDAARAITRAMEKLG-NQNLRS----------KSNFEGVSGMVKFSKKNG 240
           TKASIFAIRAYDAARA+TRAMEKLG ++ LRS          +SNFEGV GMVKFSK+NG
Sbjct: 181 TKASIFAIRAYDAARAVTRAMEKLGDDKKLRSSSKQLREKILESNFEGVGGMVKFSKQNG 240

Query: 241 MLISESPNFKIIKVVDQTYKDVGFWTPNLG------------------------------ 300
           MLISESPNFKI+KVVDQTYK+VGFWTPNLG                              
Sbjct: 241 MLISESPNFKIVKVVDQTYKEVGFWTPNLGFVENYVEIINKTTTELVKHNKGNLRKIVSV 300

Query: 301 -----------EIFDNNHGKKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVM 360
                      E FDN+HGKK+L FAVPEEAACKE VKVSQHLNGDYITGFSINLFRAVM
Sbjct: 301 GNLSRLKTSSSENFDNDHGKKRLTFAVPEEAACKELVKVSQHLNGDYITGFSINLFRAVM 360

Query: 361 NNINISDFSCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEI 420
           NNIN SDFS YELVPFKG YDDM+KAVSNK Y GA+GDI ILAHRYKYVDFTVSYL+TEI
Sbjct: 361 NNINTSDFSSYELVPFKGRYDDMIKAVSNKTYFGAVGDIIILAHRYKYVDFTVSYLQTEI 420

Query: 421 VMVVKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWF 480
           VMVV+QKDDKWKKIWAFM AF+L MW LIPTMHLFISFVIWLIERQNNPELEGVGNMLWF
Sbjct: 421 VMVVEQKDDKWKKIWAFMEAFELTMWLLIPTMHLFISFVIWLIERQNNPELEGVGNMLWF 480

Query: 481 SISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLK 540
           SISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISW RPSVPDV+TLK
Sbjct: 481 SISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWFRPSVPDVETLK 540

Query: 541 QMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVY 600
           QMGYNVGCNTNSFICNYL+DTL FDPTKIKKI SIDEYPKAF N TIKAAFFISPHA+VY
Sbjct: 541 QMGYNVGCNTNSFICNYLVDTLEFDPTKIKKIDSIDEYPKAFKNGTIKAAFFISPHAKVY 600

Query: 601 LAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNC 631
           LAKYCKGYTKGVSSFKLSGIGFAM KGSELASRVSASIVELTETKEIPQFES+VLA FNC
Sbjct: 601 LAKYCKGYTKGVSSFKLSGIGFAMAKGSELASRVSASIVELTETKEIPQFESDVLASFNC 660

BLAST of PI0019852 vs. NCBI nr
Match: KAE8652078.1 (hypothetical protein Csa_018677 [Cucumis sativus])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.7e-303
Identity = 544/703 (77.38%), Postives = 589/703 (83.78%), Query Frame = 0

Query: 1   MANIDNNITHPIHCIASILTHFQCLPKVTIFSQFSNDFSLSLDSLFDSFLPAGVEVEHHL 60
           MAN DN+IT+PIHCIASIL+HFQCLPKVTIF Q +ND SLSL   FDSFLPAGVEVEH L
Sbjct: 156 MANTDNDITYPIHCIASILSHFQCLPKVTIFYQITNDPSLSLHRFFDSFLPAGVEVEHRL 215

Query: 61  ALSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI-- 120
           ALS+ASNQEI+IEQELTRLMN+QR+R FIITQLSLELVDLLLTKAKKLNMVGNGYTWI  
Sbjct: 216 ALSSASNQEIVIEQELTRLMNNQRSRNFIITQLSLELVDLLLTKAKKLNMVGNGYTWIIS 275

Query: 121 ------------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTK 180
                             +MEGVIGF TYFNDS+KSFKSFETKFKKIY LEYPQEEEPTK
Sbjct: 276 HEVFDLISYLDSSSSLLSKMEGVIGFGTYFNDSRKSFKSFETKFKKIYRLEYPQEEEPTK 335

Query: 181 ASIFAIRAYDAARAITRAMEKLGNQNLRS----------KSNFEGVSGMVKFSKKNGMLI 240
           ASIFAIRAYDAAR I RAME+LG++NLRS          +SNFEGVSGMVKFSKKNGMLI
Sbjct: 336 ASIFAIRAYDAARNIIRAMERLGDENLRSSSKQLMDKILESNFEGVSGMVKFSKKNGMLI 395

Query: 241 SESPNFKIIKVVDQTYKDVGFWTPNLG--------------------------------- 300
           SESPNFKI+KVVDQTYK+VGFWTPNLG                                 
Sbjct: 396 SESPNFKIVKVVDQTYKEVGFWTPNLGFVENYVEIISKTTTKLVKHSKGNLRKNLSVGDL 455

Query: 301 --------EIFDNNHGKKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNI 360
                   E FDN+H KKK  FAVPE+AACKEFVKVSQHLNG+YITGF++ LFRAVMNNI
Sbjct: 456 SRPKTSSSENFDNHHSKKKFKFAVPEDAACKEFVKVSQHLNGNYITGFAVTLFRAVMNNI 515

Query: 361 NISDFSCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMV 420
           N+S+FS YELVP KGTY+ M++ VS KI+ GA+GDIGILA RYK+VD+TVSYLETEIVMV
Sbjct: 516 NMSEFSDYELVPLKGTYNKMIEDVSKKIFFGAVGDIGILAQRYKHVDYTVSYLETEIVMV 575

Query: 421 VKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSIS 480
           V+QKDDKWKKIWAFMGAFQL MW LIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSIS
Sbjct: 576 VQQKDDKWKKIWAFMGAFQLTMWLLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSIS 635

Query: 481 IIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMG 540
           I+FYMHREP+KNG+ARLVLGPWLFAILV+TASFTASL SMMT SW RPSVPDV+TL++MG
Sbjct: 636 IVFYMHREPVKNGMARLVLGPWLFAILVITASFTASLASMMTNSWLRPSVPDVETLRKMG 695

Query: 541 YNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAK 600
           +NVGCNTNSFIC+YL DTL FDP KIKKI  +DEYPKAF++ TIKAAFFISPHA VYLAK
Sbjct: 696 HNVGCNTNSFICSYLADTLKFDPEKIKKIDLVDEYPKAFESGTIKAAFFISPHARVYLAK 755

Query: 601 YCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNCSSN 633
            CKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTET EIPQFESNVLA FNCSSN
Sbjct: 756 NCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETNEIPQFESNVLASFNCSSN 815

BLAST of PI0019852 vs. NCBI nr
Match: XP_031736600.1 (glutamate receptor 2.5 [Cucumis sativus])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.7e-303
Identity = 544/703 (77.38%), Postives = 589/703 (83.78%), Query Frame = 0

Query: 1   MANIDNNITHPIHCIASILTHFQCLPKVTIFSQFSNDFSLSLDSLFDSFLPAGVEVEHHL 60
           MAN DN+IT+PIHCIASIL+HFQCLPKVTIF Q +ND SLSL   FDSFLPAGVEVEH L
Sbjct: 1   MANTDNDITYPIHCIASILSHFQCLPKVTIFYQITNDPSLSLHRFFDSFLPAGVEVEHRL 60

Query: 61  ALSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI-- 120
           ALS+ASNQEI+IEQELTRLMN+QR+R FIITQLSLELVDLLLTKAKKLNMVGNGYTWI  
Sbjct: 61  ALSSASNQEIVIEQELTRLMNNQRSRNFIITQLSLELVDLLLTKAKKLNMVGNGYTWIIS 120

Query: 121 ------------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTK 180
                             +MEGVIGF TYFNDS+KSFKSFETKFKKIY LEYPQEEEPTK
Sbjct: 121 HEVFDLISYLDSSSSLLSKMEGVIGFGTYFNDSRKSFKSFETKFKKIYRLEYPQEEEPTK 180

Query: 181 ASIFAIRAYDAARAITRAMEKLGNQNLRS----------KSNFEGVSGMVKFSKKNGMLI 240
           ASIFAIRAYDAAR I RAME+LG++NLRS          +SNFEGVSGMVKFSKKNGMLI
Sbjct: 181 ASIFAIRAYDAARNIIRAMERLGDENLRSSSKQLMDKILESNFEGVSGMVKFSKKNGMLI 240

Query: 241 SESPNFKIIKVVDQTYKDVGFWTPNLG--------------------------------- 300
           SESPNFKI+KVVDQTYK+VGFWTPNLG                                 
Sbjct: 241 SESPNFKIVKVVDQTYKEVGFWTPNLGFVENYVEIISKTTTKLVKHSKGNLRKNLSVGDL 300

Query: 301 --------EIFDNNHGKKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNI 360
                   E FDN+H KKK  FAVPE+AACKEFVKVSQHLNG+YITGF++ LFRAVMNNI
Sbjct: 301 SRPKTSSSENFDNHHSKKKFKFAVPEDAACKEFVKVSQHLNGNYITGFAVTLFRAVMNNI 360

Query: 361 NISDFSCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMV 420
           N+S+FS YELVP KGTY+ M++ VS KI+ GA+GDIGILA RYK+VD+TVSYLETEIVMV
Sbjct: 361 NMSEFSDYELVPLKGTYNKMIEDVSKKIFFGAVGDIGILAQRYKHVDYTVSYLETEIVMV 420

Query: 421 VKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSIS 480
           V+QKDDKWKKIWAFMGAFQL MW LIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSIS
Sbjct: 421 VQQKDDKWKKIWAFMGAFQLTMWLLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSIS 480

Query: 481 IIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMG 540
           I+FYMHREP+KNG+ARLVLGPWLFAILV+TASFTASL SMMT SW RPSVPDV+TL++MG
Sbjct: 481 IVFYMHREPVKNGMARLVLGPWLFAILVITASFTASLASMMTNSWLRPSVPDVETLRKMG 540

Query: 541 YNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAK 600
           +NVGCNTNSFIC+YL DTL FDP KIKKI  +DEYPKAF++ TIKAAFFISPHA VYLAK
Sbjct: 541 HNVGCNTNSFICSYLADTLKFDPEKIKKIDLVDEYPKAFESGTIKAAFFISPHARVYLAK 600

Query: 601 YCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNCSSN 633
            CKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTET EIPQFESNVLA FNCSSN
Sbjct: 601 NCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETNEIPQFESNVLASFNCSSN 660

BLAST of PI0019852 vs. NCBI nr
Match: XP_038884567.1 (glutamate receptor 2.5-like [Benincasa hispida])

HSP 1 Score: 985.7 bits (2547), Expect = 1.8e-283
Identity = 513/693 (74.03%), Postives = 565/693 (81.53%), Query Frame = 0

Query: 5   DNNITHPIHCIASILTHFQCLPKVTIFSQFSNDFSLSLDSLFDSFLPAGVEVEHHLALSA 64
           +NNITHPIHCIASILT+F+CLPKVTIF QF+N    S   LFDSFL AG+EVEHHLALS 
Sbjct: 157 NNNITHPIHCIASILTYFECLPKVTIFYQFTN---TSAHCLFDSFLLAGIEVEHHLALSL 216

Query: 65  ASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI------ 124
           ASNQEILIE+EL ++M+SQRNRVFI+TQLSLELVDLLLTKAKK+NMVGNGYTWI      
Sbjct: 217 ASNQEILIEEELKKIMSSQRNRVFIVTQLSLELVDLLLTKAKKMNMVGNGYTWIVSHEIF 276

Query: 125 --------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKASIF 184
                         +MEGVIGF+TYFND+K+SFK FETKFKKIY LEYPQEEEPTKASI 
Sbjct: 277 DLISSLDSSSSLLNKMEGVIGFQTYFNDTKRSFKIFETKFKKIYKLEYPQEEEPTKASIL 336

Query: 185 AIRAYDAARAITRAMEKLGNQNLRS----------KSNFEGVSGMVKFSKKNGMLISESP 244
           AIRAYDAA AITRAMEKLG +NLRS          +SNFEGV GMV+FSKKNGMLIS+SP
Sbjct: 337 AIRAYDAALAITRAMEKLGTENLRSSSEQLMKKILESNFEGVGGMVRFSKKNGMLISQSP 396

Query: 245 NFKIIKVVDQTYKDVGFWTPNLG------------------------------------- 304
            FKIIKVVDQTYK+VGFWTP LG                                     
Sbjct: 397 KFKIIKVVDQTYKEVGFWTPILGFVERVIVEINKPTTNLKSNMRNLRNYVGVRDLSRLKA 456

Query: 305 ---EIFDNNHGKKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNINISDF 364
              E FD +H +K+L FAVPEE ACKEFVKVS HL G+YITG+SI++FRAVMNN+N+S  
Sbjct: 457 SPSEKFD-HHEEKRLKFAVPEEGACKEFVKVSHHLYGNYITGYSIDVFRAVMNNMNMSHP 516

Query: 365 SCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMVVKQKD 424
             Y+LVPFKGTYD+M++AVSNK Y GA+GDIGILA RYKYVDFTVSYLETEIVMVVK+K 
Sbjct: 517 LSYDLVPFKGTYDEMIEAVSNKTYIGAVGDIGILARRYKYVDFTVSYLETEIVMVVKEKH 576

Query: 425 DKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSISIIFYM 484
           +KWKKIWAFM AFQ  MW LIPTMHLFISFVIW IERQNN EL+G+GNMLWFS+SIIFYM
Sbjct: 577 EKWKKIWAFMEAFQFTMWLLIPTMHLFISFVIWFIERQNNEELKGLGNMLWFSVSIIFYM 636

Query: 485 HREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMGYNVGC 544
           HREP+KNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRP VPDV TLKQMG  VGC
Sbjct: 637 HREPVKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPLVPDVDTLKQMGATVGC 696

Query: 545 NTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAKYCKGY 604
           NTNSFICNYL +TL FDPTKIKKI S++EYP AF+N +IKAAFFISPHA+V+LAKYCKGY
Sbjct: 697 NTNSFICNYLYETLQFDPTKIKKINSLNEYPNAFENGSIKAAFFISPHAKVFLAKYCKGY 756

Query: 605 TKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNCSSNGKGHG 628
           T+GVSSFKL+GIGFA+ KGS L S VSASIVELTETKEIPQFESNV+A FNCSS GKG G
Sbjct: 757 TRGVSSFKLTGIGFALAKGSGLTSLVSASIVELTETKEIPQFESNVIASFNCSSTGKGEG 816

BLAST of PI0019852 vs. NCBI nr
Match: XP_022996842.1 (glutamate receptor 2.5-like [Cucurbita maxima])

HSP 1 Score: 816.2 bits (2107), Expect = 1.9e-232
Identity = 435/682 (63.78%), Postives = 514/682 (75.37%), Query Frame = 0

Query: 8   ITHPIHCIASILTHFQCLPKVTIFSQFSND------FSLSLDSLFDSFLPAGVEVEHHLA 67
           ITH I CIASILTHFQ   KVTIF   SN        ++S + LFDS   A VEVEH LA
Sbjct: 163 ITHRIQCIASILTHFQ-WRKVTIFYDISNTDHSPIYSAVSANRLFDSLRLADVEVEHRLA 222

Query: 68  LSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI--- 127
           LS++SNQEILIEQEL +LMNSQRNRVF++TQL +EL  L+L +AKKLNMVGNGY WI   
Sbjct: 223 LSSSSNQEILIEQELKKLMNSQRNRVFVVTQLEVELAVLVLEQAKKLNMVGNGYVWIVSN 282

Query: 128 ---------------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKASI 187
                          +MEGVIGF TYF+D+K SFKSFETKFKK+Y LEYPQEEEPT+ASI
Sbjct: 283 DVFDAIDSLDSSFWYKMEGVIGFRTYFDDTKNSFKSFETKFKKMYRLEYPQEEEPTRASI 342

Query: 188 FAIRAYDAARAITRAM-EKLGNQNLRSK---SNFEGVSGMVKFSKKNGMLISESPNFKII 247
             +RAYDAARAITRAM E L    +  K   SNFEG+SGMV+F  KNGMLIS+SPNFKI 
Sbjct: 343 SVVRAYDAARAITRAMGENLSWSEVLEKIGESNFEGLSGMVRF--KNGMLISQSPNFKIS 402

Query: 248 KVVDQTYKDVGFWTPNLGEI----------------------------------FDNNHG 307
           KVV Q++K+VGFWTP LG +                                   +N  G
Sbjct: 403 KVVGQSFKEVGFWTPKLGFVERFVEVNKTTTKLKPKFGNLGNVAVGDLPRLTTSSENCDG 462

Query: 308 KKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNINISDFSCYELVPFKGT 367
           +K+L FAVPEE AC+E VKVS+HL G Y+TGFSIN+FRAVM+NINIS    Y+L+PFKG 
Sbjct: 463 EKRLRFAVPEEGACQEIVKVSKHLEGYYVTGFSINVFRAVMSNINISHPLSYDLLPFKGK 522

Query: 368 YDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMVVKQKDDKWKKIWAFMG 427
           Y+DM++AV NK Y GA+G+IGIL +R+  VDFTVSYLETEIVMVVK+K  +WK++WAF  
Sbjct: 523 YEDMLEAVRNKTYDGAVGEIGILPNRFDIVDFTVSYLETEIVMVVKEKRSRWKQLWAFTD 582

Query: 428 AFQLKMWFLIPTMHLFISFVIWLIERQNNPELEGVGNMLWFSISIIFYMHREPLKNGLAR 487
           AF++ MW LIPTMHLFISF +WLIERQN+ EL+G GNMLWFS+SIIFYMHREP+KNGLAR
Sbjct: 583 AFEVSMWLLIPTMHLFISFAVWLIERQNSEELKGFGNMLWFSVSIIFYMHREPVKNGLAR 642

Query: 488 LVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMGYNVGCNTNSFICNYLI 547
           LVLGPWLF ILVVTASFT+SLTSMMT+SW RPSV DV  LK++   VGCN  SFIC+YL 
Sbjct: 643 LVLGPWLFGILVVTASFTSSLTSMMTVSWYRPSVLDVVKLKEINAVVGCNARSFICDYLK 702

Query: 548 DTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAKYCKGYTKGVSSFKLSG 607
            TL F+P+KIK+I S++ YPKAF+++TIKAAFFISPHA+V+LAK C+GYTKGVSS+KLSG
Sbjct: 703 KTLKFEPSKIKRIDSLNNYPKAFEDNTIKAAFFISPHADVFLAKNCRGYTKGVSSYKLSG 762

Query: 608 IGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPFNCSSNGKGHGLGLGPEPFMGL 628
           +GFA  KGS LA++VSASIVELTETKE+PQF+ N L  FNC +  +       P PFMGL
Sbjct: 763 VGFAFAKGSPLAAKVSASIVELTETKEMPQFDPNYLVSFNCPTAKE-------PGPFMGL 822

BLAST of PI0019852 vs. TAIR 10
Match: AT5G11210.1 (glutamate receptor 2.5 )

HSP 1 Score: 250.4 bits (638), Expect = 3.9e-66
Identity = 204/712 (28.65%), Postives = 339/712 (47.61%), Query Frame = 0

Query: 12  IHCIASILTHFQCLPKVTIFSQFSNDFSLS-LDSLFDSFLPAGVEVEHHLALSAASNQEI 71
           +  I++I+  F+    V I+    N+F    L +L D+F    V + +  A+S   + + 
Sbjct: 83  VQAISAIIESFRWREVVPIY--VDNEFGEGILPNLVDAFQEINVRIRYRSAISLHYSDD- 142

Query: 72  LIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI------------ 131
            I++EL +LM +   RVFI+  L  +L   L + AK+++M+  GY WI            
Sbjct: 143 QIKKELYKLM-TMPTRVFIVHMLP-DLGSRLFSIAKEIDMLSKGYVWIVTNGIADLMSIM 202

Query: 132 ------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKASIFAIRAYDAA 191
                  M GV+G +TYF  SK+     E +++K +  E        + + FA  AYDAA
Sbjct: 203 GESSLVNMHGVLGVKTYFAKSKE-LLHLEARWQKRFGGE--------ELNNFACWAYDAA 262

Query: 192 RAITRAMEKLGNQNLR---------------------------------SKSNFEGVSGM 251
            A+  ++E++ + N+                                  S  +F+GV+G 
Sbjct: 263 TALAMSVEEIRHVNMSFNTTKEDTSRDDIGTDLDELGVALSGPKLLDALSTVSFKGVAG- 322

Query: 252 VKFSKKNGMLISESPNFKIIKVVDQTYKDVGFWTPNLGEI-------------------- 311
            +F  KNG L  E+  FKII + +   + VGFW   +G +                    
Sbjct: 323 -RFQLKNGKL--EATTFKIINIEESGERTVGFWKSKVGLVKSLRVDKVSHSSRRLRPIIW 382

Query: 312 ----------FDNNHGKKKLNFAVPEEAACKEFVKVS--QHLNGDYITGFSINLFRAVMN 371
                     ++     KKL  AVP++     FV+V+  ++ N   +TGF I++F  VM+
Sbjct: 383 PGDTIFVPKGWEFPTNAKKLRIAVPKKDGFNNFVEVTKDENTNVPTVTGFCIDVFNTVMS 442

Query: 372 NINISDFSCYELVPF-------KGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVS 431
            +  +    YE +PF       +G+YD+M+  V    + GA+GD  ILA+R  YVDF + 
Sbjct: 443 QMPYA--VSYEYIPFDTPDGKPRGSYDEMVYNVFLGEFDGAVGDTTILANRSHYVDFALP 502

Query: 432 YLETEIVMVVKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPE---- 491
           Y ET IV +V  KD K K  W F+     ++W +     L+I  ++W+ E Q + E    
Sbjct: 503 YSETGIVFLVPVKDGKEKGEWVFLKPLTKELWLVTAASFLYIGIMVWIFEYQADEEFREQ 562

Query: 492 --LEGVGNMLWFSISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISW 551
             ++ + ++ +FS S +F+ HR P ++   R+++  W F +L++T S+TA+LTSM+T+  
Sbjct: 563 MIIDKISSVFYFSFSTLFFAHRRPSESFFTRVLVVVWCFVLLILTQSYTATLTSMLTVQE 622

Query: 552 SRPSVPDVKTLKQMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAF----DN 611
            RP+V  +  L++ G N+G  T SF    L   + FD +++K   S +E  + F     N
Sbjct: 623 LRPTVRHMDDLRKSGVNIGYQTGSFTFERL-KQMRFDESRLKTYNSPEEMRELFLHKSSN 682

Query: 612 DTIKAAFFISPHAEVYLAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTET 621
             I AAF    + ++++AKYC  Y+    +FK  G GFA   GS L S +S  I+ +TE 
Sbjct: 683 GGIDAAFDEVAYIKLFMAKYCSEYSIIEPTFKADGFGFAFPLGSPLVSDISRQILNITEG 742

BLAST of PI0019852 vs. TAIR 10
Match: AT2G29120.1 (glutamate receptor 2.7 )

HSP 1 Score: 233.4 bits (594), Expect = 5.0e-61
Identity = 195/704 (27.70%), Postives = 318/704 (45.17%), Query Frame = 0

Query: 12  IHCIASILTHFQCLPKVTIFSQFSNDFSLS-LDSLFDSFLPAGVEVEHHLALSAASNQEI 71
           +  IA+I+  F     V I+    N+F    L  L D+       V +   +   +N + 
Sbjct: 159 VKAIAAIVKSFGWRNVVAIY--VDNEFGEGILPLLTDALQDVQAFVVNRCLIPQEANDDQ 218

Query: 72  LIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWI------------ 131
           ++ +EL +LM  Q  RVF++  +   L      KA+++ M+  GY W+            
Sbjct: 219 IL-KELYKLMTMQ-TRVFVV-HMPPTLGFRFFQKAREIGMMEEGYVWLLTDGVMNLLKSN 278

Query: 132 -------QMEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKASIFAIRAYDA 191
                   M+GV+G  ++   SKK  K+F  +++K+    +P++    + +IFA+RAYD+
Sbjct: 279 ERGSSLENMQGVLGVRSHIPKSKK-LKNFRLRWEKM----FPKKGNDEEMNIFALRAYDS 338

Query: 192 ARAITRAMEKLGNQNLR------------------------------SKSNFEGVSGMVK 251
             A+  A+EK   ++LR                              S   F G++G  +
Sbjct: 339 ITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALSNVRFNGLAG--E 398

Query: 252 FSKKNGMLISESPNFKIIKVVDQTYKDVGFWTPN------------------LGEIFDNN 311
           F   NG L  ES  F +I ++    + +G W P+                  LG +    
Sbjct: 399 FELINGQL--ESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTSVLGERLGPVIWPG 458

Query: 312 HGK------------KKLNFAVPEEAACKEFV--KVSQHLNGDYITGFSINLFRAVMNNI 371
             K            K L   +P +    EFV  K+    N    TG+ I +F AV+  +
Sbjct: 459 KSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEIFEAVLKKL 518

Query: 372 NISDFSCY-ELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVM 431
             S    Y   +     YD+M+  V    Y   +GD+ I+A+R  YVDFT+ Y E+ + M
Sbjct: 519 PYSVIPKYIAFLSPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVDFTLPYTESGVSM 578

Query: 432 VVKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEG-----VGNM 491
           +V  KD+  K  W F+  + L +W       +FI F++W++E + N +  G     +G  
Sbjct: 579 MVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTDFRGPPHHQIGTS 638

Query: 492 LWFSISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVK 551
            WF+ S + + HRE + + LAR V+  W F +LV+  S+TA+LTS  T+   +P+V + K
Sbjct: 639 FWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFTVKLLQPTVTNWK 698

Query: 552 TLKQMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHA 611
            L +   N+G    +F+   L+ +  FD +++K  GS  E  + F N TI A+F    + 
Sbjct: 699 DLIKFNKNIGYQRGTFV-RELLKSQGFDESQLKPFGSAVECDELFSNGTITASFDEVAYI 758

Query: 612 EVYLAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESN-VLA 626
           +V L++    YT    SFK +G GF   K S L   VS +I+ +T+ +E+   E+     
Sbjct: 759 KVILSQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNVTQGEEMQHIENKWFKK 818

BLAST of PI0019852 vs. TAIR 10
Match: AT2G29110.1 (glutamate receptor 2.8 )

HSP 1 Score: 230.3 bits (586), Expect = 4.2e-60
Identity = 195/703 (27.74%), Postives = 315/703 (44.81%), Query Frame = 0

Query: 9   THPIHCIASILTHFQCLPKVTIFSQFSNDFSLS-LDSLFDSFLPAGVEVEHHLALSAASN 68
           ++ +  IA+I   F     V I+    N+     +  LFD+     V+V+  +  S A++
Sbjct: 150 SYQVKAIAAIFESFGWRSVVAIY--VDNELGEGIMPYLFDAL--QDVQVDRSVIPSEAND 209

Query: 69  QEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWIQ-------- 128
            +IL  +EL +LM  Q  RVF++  ++  L   +  KA ++ M+  GY W+         
Sbjct: 210 DQIL--KELYKLMTRQ-TRVFVV-HMASRLASRIFEKATEIGMMEEGYVWLMTNGMTHMM 269

Query: 129 -----------MEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTKASIFAIRA 188
                      ++GV+G  ++   S K  + F  ++K+ +  E P   +    SIF + A
Sbjct: 270 RHIHHGRSLNTIDGVLGVRSHVPKS-KGLEDFRLRWKRNFKKENPWLRD--DLSIFGLWA 329

Query: 189 YDAARAITRAMEKLG-----NQNLRSKSNFEGVSGMVKFSKKNGMLIS------------ 248
           YD+  A+  A+EK         N    SN     G +  S+    L+             
Sbjct: 330 YDSTTALAMAVEKTNISSFPYNNASGSSNNMTDLGTLHVSRYGPSLLEALSEIRFNGLAG 389

Query: 249 ---------ESPNFKIIKVVDQTYKDVGFWTPNLGEIFDNNH----------------GK 308
                    ESP F+II  V    + VGFWTP+ G +  N++                GK
Sbjct: 390 RFNLIDRQLESPKFEIINFVGNEERIVGFWTPSNGLVNVNSNKTTSFTGERFGPLIWPGK 449

Query: 309 --------------KKLNFAVPEEAACKEFVKVSQHLNGDYIT--GFSINLFRAVMNNIN 368
                         KK+   VP +     FV+V      +  T  G++I++F A +  + 
Sbjct: 450 STIVPKGWEIPTNGKKIKVGVPVKKGFFNFVEVITDPITNITTPKGYAIDIFEAALKKLP 509

Query: 369 ISDF-SCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMV 428
            S     Y        YDD++  V N      +GD+ I A+R  Y DFT+ Y E+ + M+
Sbjct: 510 YSVIPQYYRFESPDDDYDDLVYKVDNGTLDAVVGDVTITAYRSLYADFTLPYTESGVSMM 569

Query: 429 VKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEG-----VGNML 488
           V  +D++ K  W F+  + L +W       + I FV+WL E + N +  G     +G   
Sbjct: 570 VPVRDNENKNTWVFLKPWGLDLWVTTACFFVLIGFVVWLFEHRVNTDFRGPPHHQIGTSF 629

Query: 489 WFSISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKT 548
           WFS S + + HRE + + LAR V+  W F +LV+T S+TA+LTS +T+   +P+  +VK 
Sbjct: 630 WFSFSTMVFAHREKVVSNLARFVVVVWCFVVLVLTQSYTANLTSFLTVQRFQPAAINVKD 689

Query: 549 LKQMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAE 608
           L + G  VG    +F+ ++LI    F+ +K+K  GS +E      N +I AAF    +  
Sbjct: 690 LIKNGDYVGYQHGAFVKDFLIKE-GFNVSKLKPFGSSEECHALLSNGSISAAFDEVAYLR 749

Query: 609 VYLAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFESNVLAPF 626
             L++YC  Y     +FK +G GFA  + S L   VS +I+ +T+  E+   E+      
Sbjct: 750 AILSQYCSKYAIVEPTFKTAGFGFAFPRNSPLTGDVSKAILNVTQGDEMQHIENKWFMKQ 809

BLAST of PI0019852 vs. TAIR 10
Match: AT2G29100.1 (glutamate receptor 2.9 )

HSP 1 Score: 223.8 bits (569), Expect = 3.9e-58
Identity = 199/712 (27.95%), Postives = 326/712 (45.79%), Query Frame = 0

Query: 2   ANIDNNITHPIHCIASILTHFQCLPKVTIF--SQFSNDFSLSLDSLFDSFLPAGVEVEHH 61
           A ID+  +  +  IASI   F+    V I+  ++F   F   +  LFD+     VEV+  
Sbjct: 142 ATIDD--SSQVRAIASIFKFFRWRRVVAIYVDNEFGEGF---MPFLFDAL--QDVEVKRS 201

Query: 62  LALSAASNQEILIEQELTRLMNSQRNRVFIITQLSLELVDLLLTKAKKLNMVGNGYTWIQ 121
           +    A + E  I++EL +LM  Q  RVF++  +   L   +   A+ + M+  GY W+ 
Sbjct: 202 VIPPEAIDDE--IQKELRKLMERQA-RVFVV-HMESSLALRVFQIARDIGMMEEGYVWLM 261

Query: 122 -------------------MEGVIGFETYFNDSKKSFKSFETKFKKIYNLEYPQEEEPTK 181
                              +EGV+G  ++   SK+    F  ++K+ +  E P   +   
Sbjct: 262 TNGMTHMMRHINNGRSLNTIEGVLGVRSHVPKSKE-LGDFRLRWKRTFEKENPSMRD--D 321

Query: 182 ASIFAIRAYDAARAITRAMEK--------------------LGN----------QNLRSK 241
            ++FA+ AYD+  A+ +A+EK                    LGN          Q   S+
Sbjct: 322 LNVFALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAFSE 381

Query: 242 SNFEGVSGMVKFSKKNGMLISESPNFKIIKVVDQTYKDVGFWTPNLGEIFDNNHGK---- 301
             F G++G  +F   +G L  +SP F+II  V    + +GFWTP  G +   +  K    
Sbjct: 382 VRFNGLAG--EFKLIDGQL--QSPKFEIINFVGNEERIIGFWTPRDGLMDATSSNKKTLG 441

Query: 302 -------------------KKLNFAVPEEAACKEFVKVSQH--LNGDYITGFSINLFRAV 361
                              KKL   VP +    +FVKV+ +   N    TG++I +F A 
Sbjct: 442 PVIWPGKSKIVPKGWEIPGKKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIEIFEAA 501

Query: 362 MNNINISDFSCYELVPFKGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETE 421
           +  +       Y        Y++++  V +K +   +GDI I A+R  Y DFT+ + E+ 
Sbjct: 502 LKELPYLVIPEYVSFESPNNYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLPFTESG 561

Query: 422 IVMVVKQKDDKWKKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEG-----V 481
           + M+V  +D++ K  W F+  + L++W       +FI FV+WL E + N +  G     +
Sbjct: 562 VSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGPPQYQI 621

Query: 482 GNMLWFSISIIFYMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVP 541
           G  LWFS S + + HRE + + LAR V+  W F +LV+T S+TASLTS +T+   +P+V 
Sbjct: 622 GTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSLQPTVT 681

Query: 542 DVKTLKQMGYNVGCNTNSFICNYLIDTLNFDPTKIKKIGS---IDEYPKAFDNDTIKAAF 601
           +V  L +    VG    +F+ + L+  L F   ++K   S    D+      +  I AAF
Sbjct: 682 NVNDLIKNRDCVGYQGGAFVKDILLG-LGFHEDQLKPFDSAKDADDLLSKGKSKGIAAAF 741

Query: 602 FISPHAEVYLAKYCKGYTKGVSSFKLSGIGFAMEKGSELASRVSASIVELTETKEIPQFE 622
               + +  L++ C  Y     +FK  G GFA  K S L    S +I+ LT+     Q E
Sbjct: 742 DEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNVTQQIE 801

BLAST of PI0019852 vs. TAIR 10
Match: AT4G35290.2 (glutamate receptor 2 )

HSP 1 Score: 214.9 bits (546), Expect = 1.8e-55
Identity = 174/629 (27.66%), Postives = 283/629 (44.99%), Query Frame = 0

Query: 101 LLTKAKKLNMVGNGYTWI---------------------QMEGVIGFETYFNDSKKSFKS 160
           +  +A+KL M+  GY WI                      + GV+    +  +SKK  K 
Sbjct: 238 IFEEAQKLGMMEKGYVWIATTWLTSLLDSVNPLPAKTAESLRGVLTLRIHTPNSKKK-KD 297

Query: 161 FETKFKKIYNLEYPQEEEPTKASIFAIRAYDAARAITRAMEKL----------------- 220
           F  ++ K+ N            +++ + AYD    I RA+++L                 
Sbjct: 298 FVARWNKLSN-------GTVGLNVYGLYAYDTVWIIARAVKRLLDSRANISFSSDPKLTS 357

Query: 221 ----GNQNLRSKS---------------NFEGVSGMVKFSKKNGMLISESPNFKIIKVVD 280
               G+ NL + S               N  GV+G ++F     M+    P++ II VVD
Sbjct: 358 MKGGGSLNLGALSIFDQGSQFLDYIVNTNMTGVTGQIQFLPDRSMI---QPSYDIINVVD 417

Query: 281 QTYKDVGFW---------------------------------------TPNLGEIFDNNH 340
             ++ +G+W                                       TP  G +F NN 
Sbjct: 418 DGFRQIGYWSNHSGLSIIPPESLYKKLSNRSSSNQHLNNVTWPGGTSETPR-GWVFPNN- 477

Query: 341 GKKKLNFAVPEEAACKEFVKVSQHLNGDYITGFSINLFRAVMNNINISDFSCYELVPF-- 400
             ++L   VP+ A+ KEF  VS+    + + G++I++F A +  I+      +E V F  
Sbjct: 478 -GRRLRIGVPDRASFKEF--VSRLDGSNKVQGYAIDVFEAAVKLISYP--VPHEFVLFGD 537

Query: 401 ---KGTYDDMMKAVSNKIYSGAIGDIGILAHRYKYVDFTVSYLETEIVMV--VKQKDDKW 460
                 +++ +  V+  ++   +GDI I+  R + VDFT  Y+E+ +V+V  V + +D  
Sbjct: 538 GLKNPNFNEFVNNVTIGVFDAVVGDIAIVTKRTRIVDFTQPYIESGLVVVAPVTKLND-- 597

Query: 461 KKIWAFMGAFQLKMWFLIPTMHLFISFVIWLIERQNNPELEG-----VGNMLWFSISIIF 520
              WAF+  F   MW +     L +  VIW++E + N E  G     +  +LWFS S +F
Sbjct: 598 -TPWAFLRPFTPPMWAVTAAFFLIVGSVIWILEHRINDEFRGPPRKQIVTILWFSFSTMF 657

Query: 521 YMHREPLKNGLARLVLGPWLFAILVVTASFTASLTSMMTISWSRPSVPDVKTLKQMGYNV 580
           + HRE   + L R VL  WLF +L++T+S+TASLTS++T+      +  V TL      V
Sbjct: 658 FSHRENTVSTLGRAVLLIWLFVVLIITSSYTASLTSILTVQQLNSPIRGVDTLISSSGRV 717

Query: 581 GCNTNSFICNYLIDTLNFDPTKIKKIGSIDEYPKAFDNDTIKAAFFISPHAEVYLAKYCK 618
           G    S+  NY+ID LN   +++  +GS  EY  A  N T+ A     P+ +++L+++C 
Sbjct: 718 GFQVGSYAENYMIDELNIARSRLVPLGSPKEYAAALQNGTVAAIVDERPYVDLFLSEFC- 777