PI0019666 (gene) Melon (PI 482460) v1

Overview
NamePI0019666
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionAllene oxide synthase
Locationchr03: 1644644 .. 1646623 (-)
RNA-Seq ExpressionPI0019666
SyntenyPI0019666
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCATATTTTCATATAAATACCAAAGCAACTATCTTCTTCATTCATCACAAACAACAACACAAAACCAAAACACTCCCGTTTCTCTCTGAATCAAACTACCGTTTCAGAGAATTCCGGCGATAATGTCCTCCATTGTCATTCCTTCTCTTCAACCTCACTTGCGATTCCCATCGCAAGAAACGCCTCAAAGATCTCGTTCTAGAGTTGGCTTCGTTTCCCTACGTCCAATCTACGCCGCCGACGGAGTTTCTTCCTCGTCTTCTTCCTCTCTTCAAGTGCCGCAGCGGATTGTTTCGCCCCCGGAACCCACCAAGCTTCCTTTGAGGAAGATTCCTGGTGATTATGGGCCACCGATGTTTGGGGCGTTGAAGGACAGACACGATTATTTCTATAATCAGGGGAGGGAAGAGTATTTGAAATCTCGAATGCTCCGCTATGAATCCACTGTATATAGAACTAATATGCCACCGGGTCCATTTATCACTTCCGATTCTCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGACCATTCTAAAGTTGAGAAGAAGGATCTCTTCACCGGAACTTACATGCCTGTAACAGAGCTCACCGGCGGTTACAGGGTGCTTTCTTATATTGACCCATCCGAGCCCGATCACGCTAAGCTTAAACAGTTGATTTTCTTTCTCCTCAAGCACCGCCGGGATAAAATTATGCCGGAATTTCACTCTACTTTTTCTGAGCTATTCGAGACTCTTGAAAAGGATTTGGCTGCTGCTGGTAGAGCAGAGTACAATGCTTCCGGTGAACAAGCGGCGTTTAATTTCTTGGCTCGGTCTCTTTTCGGCGCTAATCCGGTAGATTCCAAATTGGGTCGTGACGCTCCGAAATTGATAGCGAAATGGGTTTTGTTCCAGCTTGGCCCTGTTCTGAGTCTCGGCCTCCCCAAGGTCGTCGAGGAGCTTCTCCTCCGCACGGTCCGGCTCCCGCCGGCGTTGATTAAAGCCGATTACAGGCGGTTGTACGACTTCTTTTACAAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGACTGGGGATTTCGAGAGAAGAAGCCTGTCACAACTTGCTATTCACAACTTGTTTTAATTCATTTGGAGGGATGAAGATCTTTTTCCCCAATATGATCAAATGGATCGGCCGAGCAGGAGTGAATCTCCACACCCAACTCGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGGGCTATGGAACAGATGCCGCTGATGAAATCAGTGGTGTATGAAGCGTTCAGAATCGAGCCGCCGGTTCCGGTTCAGTACGGTCGGGCGAAGAAAGACCTCGTGGTCGAAAGCCATGACGCGGCTTTCGAGATCAAAGAAGGCGAAGTGATTTGTGGGTATCAGCCATTTGCAACAAGAGATCCGAAAATCTTCGACAGAGCCGACGAGTTCGTGCCTGATCGGTTCACCGGCGACGGTGAGGAGCTGCTGAAACACGTTCTATGGTCAAACGGACCGGAAACTCAGTCTCCATCGGTTCAGAACAAGCAGTGCGCCGGAAAAGACTTCATCGTATTCATCTCTCGCCTTCTGGTCGTTGAACTTTTCCTCCGGTACGACTCTTTCGACATCGAAGCCTCAAACACTCCGTTAGGTGCCGCGGTCACCGTAACCTCCCTAAAGAAAGCAAGTTTCTGAGGGAACAAAGAAATAGAAAATAACCGTCAAGTCTGCGGTTAATTTTTGGGGTTTGTAAAAAAAGATTAAAAAAAAAAAAGTGTATAGTTAGTAATTAGAAATTGATGGGAATTTAAAGATAAAAAAGGAATGTGGGGTCCGCCAACTTTATAAGAACCAATGGAAGGAGATCTATGTGGGACCCCCAAACTTGCTGTCTTTCCTCAAGATGTAGATTGTTGAAGTTATCAATATATTACCAATAAATCTCTATTTCATGTGATT

mRNA sequence

CCCATATTTTCATATAAATACCAAAGCAACTATCTTCTTCATTCATCACAAACAACAACACAAAACCAAAACACTCCCGTTTCTCTCTGAATCAAACTACCGTTTCAGAGAATTCCGGCGATAATGTCCTCCATTGTCATTCCTTCTCTTCAACCTCACTTGCGATTCCCATCGCAAGAAACGCCTCAAAGATCTCGTTCTAGAGTTGGCTTCGTTTCCCTACGTCCAATCTACGCCGCCGACGGAGTTTCTTCCTCGTCTTCTTCCTCTCTTCAAGTGCCGCAGCGGATTGTTTCGCCCCCGGAACCCACCAAGCTTCCTTTGAGGAAGATTCCTGGTGATTATGGGCCACCGATGTTTGGGGCGTTGAAGGACAGACACGATTATTTCTATAATCAGGGGAGGGAAGAGTATTTGAAATCTCGAATGCTCCGCTATGAATCCACTGTATATAGAACTAATATGCCACCGGGTCCATTTATCACTTCCGATTCTCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGACCATTCTAAAGTTGAGAAGAAGGATCTCTTCACCGGAACTTACATGCCTGTAACAGAGCTCACCGGCGGTTACAGGGTGCTTTCTTATATTGACCCATCCGAGCCCGATCACGCTAAGCTTAAACAGTTGATTTTCTTTCTCCTCAAGCACCGCCGGGATAAAATTATGCCGGAATTTCACTCTACTTTTTCTGAGCTATTCGAGACTCTTGAAAAGGATTTGGCTGCTGCTGGTAGAGCAGAGTACAATGCTTCCGGTGAACAAGCGGCGTTTAATTTCTTGGCTCGGTCTCTTTTCGGCGCTAATCCGGTAGATTCCAAATTGGGTCGTGACGCTCCGAAATTGATAGCGAAATGGGTTTTGTTCCAGCTTGGCCCTGTTCTGAGTCTCGGCCTCCCCAAGGTCGTCGAGGAGCTTCTCCTCCGCACGGTCCGGCTCCCGCCGGCGTTGATTAAAGCCGATTACAGGCGGTTGTACGACTTCTTTTACAAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGACTGGGGATTTCGAGAGAAGAAGCCTGTCACAACTTGCTATTCACAACTTGTTTTAATTCATTTGGAGGGATGAAGATCTTTTTCCCCAATATGATCAAATGGATCGGCCGAGCAGGAGTGAATCTCCACACCCAACTCGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGGGCTATGGAACAGATGCCGCTGATGAAATCAGTGGTGTATGAAGCGTTCAGAATCGAGCCGCCGGTTCCGGTTCAGTACGGTCGGGCGAAGAAAGACCTCGTGGTCGAAAGCCATGACGCGGCTTTCGAGATCAAAGAAGGCGAAGTGATTTGTGGGTATCAGCCATTTGCAACAAGAGATCCGAAAATCTTCGACAGAGCCGACGAGTTCGTGCCTGATCGGTTCACCGGCGACGGTGAGGAGCTGCTGAAACACGTTCTATGGTCAAACGGACCGGAAACTCAGTCTCCATCGGTTCAGAACAAGCAGTGCGCCGGAAAAGACTTCATCGTATTCATCTCTCGCCTTCTGGTCGTTGAACTTTTCCTCCGGTACGACTCTTTCGACATCGAAGCCTCAAACACTCCGTTAGGTGCCGCGGTCACCGTAACCTCCCTAAAGAAAGCAAGTTTCTGAGGGAACAAAGAAATAGAAAATAACCGTCAAGTCTGCGGTTAATTTTTGGGGTTTGTAAAAAAAGATTAAAAAAAAAAAAGTGTATAGTTAGTAATTAGAAATTGATGGGAATTTAAAGATAAAAAAGGAATGTGGGGTCCGCCAACTTTATAAGAACCAATGGAAGGAGATCTATGTGGGACCCCCAAACTTGCTGTCTTTCCTCAAGATGTAGATTGTTGAAGTTATCAATATATTACCAATAAATCTCTATTTCATGTGATT

Coding sequence (CDS)

ATGTCCTCCATTGTCATTCCTTCTCTTCAACCTCACTTGCGATTCCCATCGCAAGAAACGCCTCAAAGATCTCGTTCTAGAGTTGGCTTCGTTTCCCTACGTCCAATCTACGCCGCCGACGGAGTTTCTTCCTCGTCTTCTTCCTCTCTTCAAGTGCCGCAGCGGATTGTTTCGCCCCCGGAACCCACCAAGCTTCCTTTGAGGAAGATTCCTGGTGATTATGGGCCACCGATGTTTGGGGCGTTGAAGGACAGACACGATTATTTCTATAATCAGGGGAGGGAAGAGTATTTGAAATCTCGAATGCTCCGCTATGAATCCACTGTATATAGAACTAATATGCCACCGGGTCCATTTATCACTTCCGATTCTCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGACCATTCTAAAGTTGAGAAGAAGGATCTCTTCACCGGAACTTACATGCCTGTAACAGAGCTCACCGGCGGTTACAGGGTGCTTTCTTATATTGACCCATCCGAGCCCGATCACGCTAAGCTTAAACAGTTGATTTTCTTTCTCCTCAAGCACCGCCGGGATAAAATTATGCCGGAATTTCACTCTACTTTTTCTGAGCTATTCGAGACTCTTGAAAAGGATTTGGCTGCTGCTGGTAGAGCAGAGTACAATGCTTCCGGTGAACAAGCGGCGTTTAATTTCTTGGCTCGGTCTCTTTTCGGCGCTAATCCGGTAGATTCCAAATTGGGTCGTGACGCTCCGAAATTGATAGCGAAATGGGTTTTGTTCCAGCTTGGCCCTGTTCTGAGTCTCGGCCTCCCCAAGGTCGTCGAGGAGCTTCTCCTCCGCACGGTCCGGCTCCCGCCGGCGTTGATTAAAGCCGATTACAGGCGGTTGTACGACTTCTTTTACAAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGACTGGGGATTTCGAGAGAAGAAGCCTGTCACAACTTGCTATTCACAACTTGTTTTAATTCATTTGGAGGGATGAAGATCTTTTTCCCCAATATGATCAAATGGATCGGCCGAGCAGGAGTGAATCTCCACACCCAACTCGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGGGCTATGGAACAGATGCCGCTGATGAAATCAGTGGTGTATGAAGCGTTCAGAATCGAGCCGCCGGTTCCGGTTCAGTACGGTCGGGCGAAGAAAGACCTCGTGGTCGAAAGCCATGACGCGGCTTTCGAGATCAAAGAAGGCGAAGTGATTTGTGGGTATCAGCCATTTGCAACAAGAGATCCGAAAATCTTCGACAGAGCCGACGAGTTCGTGCCTGATCGGTTCACCGGCGACGGTGAGGAGCTGCTGAAACACGTTCTATGGTCAAACGGACCGGAAACTCAGTCTCCATCGGTTCAGAACAAGCAGTGCGCCGGAAAAGACTTCATCGTATTCATCTCTCGCCTTCTGGTCGTTGAACTTTTCCTCCGGTACGACTCTTTCGACATCGAAGCCTCAAACACTCCGTTAGGTGCCGCGGTCACCGTAACCTCCCTAAAGAAAGCAAGTTTCTGA

Protein sequence

MSSIVIPSLQPHLRFPSQETPQRSRSRVGFVSLRPIYAADGVSSSSSSSLQVPQRIVSPPEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFGANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Homology
BLAST of PI0019666 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 4.4e-209
Identity = 354/533 (66.42%), Postives = 445/533 (83.49%), Query Frame = 0

Query: 2   SSIVIPSLQPHLRFPSQ-ETPQRSRSRVGFVSLRPIYAA-DGVSSSSSSSLQVPQRIVSP 61
           +S+ +PSL+  L+FPS   +  R  S    VS+RPI A+   +    SS  Q P    SP
Sbjct: 4   TSLSLPSLK--LQFPSHTSSSSRKNSSSYRVSIRPIQASVSEIPPYISSPSQSPSSSSSP 63

Query: 62  P-EPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 121
           P +  KLP +K+PGDYG P+ G  KDR DYFYNQG+ E+ KSR+ +++STV+RTNMPPGP
Sbjct: 64  PVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRTNMPPGP 123

Query: 122 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 181
           FI+ +  VVVLLDGKSFPVLFD SKVEKKDLFTGT+MP T+LTGGYRVLSY+DPSEP+HA
Sbjct: 124 FISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDPSEPNHA 183

Query: 182 KLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSL 241
           KLK+L+F+LL  RR++++PEFH+++SELFETLE +L+  G+A  NA+ +QAAFNFLARSL
Sbjct: 184 KLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGKAGLNAANDQAAFNFLARSL 243

Query: 242 FGANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 301
           +G NP D++LG D PKLI KWVLFQL P+L LGLPKV+E+L++ T RLPPAL+K DY+RL
Sbjct: 244 YGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPKVLEDLVMHTFRLPPALVKKDYQRL 303

Query: 302 YDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 361
           Y+FFY++S +V +EA+++GISREEACHNLLF TCFNSFGG+KIFFPNM+KWIGRAG  LH
Sbjct: 304 YNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNMLKWIGRAGAKLH 363

Query: 362 TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 421
           +QLA+EIR+ + +N GK+TM AME+MPLMKSVVYE+ RIEPPV  QYGRAK D+V+ESHD
Sbjct: 364 SQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGRAKHDMVIESHD 423

Query: 422 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPS 481
           A+FEIKEGE++ GYQPFAT+DPKIFDR++EFV DRF G +GE+LLKHVLWSNG ET++ S
Sbjct: 424 ASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVLWSNGSETENAS 483

Query: 482 VQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           + NKQCAGKDF+V +SRLL+VELFLRYDSF+IE   +PLGAA+T+TSL++ASF
Sbjct: 484 INNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLRRASF 534

BLAST of PI0019666 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 1.3e-195
Identity = 329/527 (62.43%), Postives = 415/527 (78.75%), Query Frame = 0

Query: 3   SIVIPSLQPHLRFPSQETPQRSRSRVGFVSLRPIYAADGVSSSSSSSLQVPQRIVSPPEP 62
           S  +P    H + PS+           + + RPI     VS S  S++++ Q       P
Sbjct: 6   SFSLPLPSLHQKIPSK-----------YSTFRPII----VSLSDKSTIEITQ-------P 65

Query: 63  TKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITS 122
            KL  R IPGDYG P  G  KDR DYFYNQG+ ++ +SR+ +Y+ST++RTNMPPGPFITS
Sbjct: 66  IKLSTRTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITS 125

Query: 123 DSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQ 182
           + +V+VLLDGKSFPVLFD SKVEKKDLFTGT++P TELTGGYR+LSY+DPSEP+H KLK+
Sbjct: 126 NPKVIVLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKK 185

Query: 183 LIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFGAN 242
           L+FFLL  RRD ++PEFH T++ELFETL+K++   G   +N+  +QAAFNFLARSLFG N
Sbjct: 186 LMFFLLSSRRDHVIPEFHETYTELFETLDKEMEEKGTVGFNSGSDQAAFNFLARSLFGVN 245

Query: 243 PVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYDFF 302
           PV++KLG D P LI KW+L QL PV++LGLPK ++++LL T RLPP L+K DY+RLYDFF
Sbjct: 246 PVETKLGTDGPALIGKWILLQLHPVITLGLPKFLDDVLLHTFRLPPILVKKDYQRLYDFF 305

Query: 303 YKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTQLA 362
           Y +S  +F EA++LGIS++EACHNLLF TCFNSFGGMKIFFPNM+K I +AGV +HT+LA
Sbjct: 306 YTNSANLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHTRLA 365

Query: 363 REIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFE 422
            EIR+ VK+ GGKITM AME+MPLMKSVVYEA R++PPV  QYGRAK+DL +ESHDA FE
Sbjct: 366 NEIRSEVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDAVFE 425

Query: 423 IKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPSVQNK 482
           +K+GE++ GYQPFAT+DPKIFDR  EFV DRF G +GE+LLKHVLWSNGPET+SP+V NK
Sbjct: 426 VKKGEILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTVGNK 485

Query: 483 QCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKA 529
           QCAGKDF+V +SRL V E FLRY + +++   + LG+++T+TSLKKA
Sbjct: 486 QCAGKDFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

BLAST of PI0019666 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 1.4e-194
Identity = 321/507 (63.31%), Postives = 414/507 (81.66%), Query Frame = 0

Query: 30  FVSLRPIYAADGVSSS--SSSSLQVPQRIVSPP---EPTKLPLRKIPGDYGPPMFGALKD 89
           F +LR + A   + +S    S +++P     PP   + T LP+R+IPGDYG P  G ++D
Sbjct: 30  FSNLRRVSAFRPIKASLFGDSPIKIPGITSQPPPSSDETTLPIRQIPGDYGLPGIGPIQD 89

Query: 90  RHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLFDHSKV 149
           R DYFYNQGREE+ KSR+ +Y+STVYR NMPPGPFI S+ RV+VLLD KSFPVLFD SKV
Sbjct: 90  RLDYFYNQGREEFFKSRLQKYKSTVYRANMPPGPFIASNPRVIVLLDAKSFPVLFDMSKV 149

Query: 150 EKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQLIFFLLKHRRDKIMPEFHSTFS 209
           EKKDLFTGTYMP TELTGGYR+LSY+DPSEP+H KLKQL+F L+K+RRD ++PEF S+F+
Sbjct: 150 EKKDLFTGTYMPSTELTGGYRILSYLDPSEPNHTKLKQLLFNLIKNRRDYVIPEFSSSFT 209

Query: 210 ELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFGANPVDSKLGRDAPKLIAKWVLFQL 269
           +L E +E DLA  G+A +N   EQAAFNFL+R+ FG  P+D+ LG+DAP LI+KWVLF L
Sbjct: 210 DLCEVVEYDLATKGKAAFNDPAEQAAFNFLSRAFFGVKPIDTPLGKDAPSLISKWVLFNL 269

Query: 270 GPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYKSSEAVFEEADRLGISREEAC 329
            P+LS+GLPK VEE  L +VRLPP L++ DY RLY+FF  ++ +V +EA++ GISR+EAC
Sbjct: 270 APILSVGLPKEVEEATLHSVRLPPLLVQNDYHRLYEFFTSAAGSVLDEAEQSGISRDEAC 329

Query: 330 HNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTQLAREIRTAVKA-NGGKITMGAMEQ 389
           HN+LF  CFNS+GG KI FP+++KWIGRAG+ LHT+LA+EIR+A+++  GGK+TM AMEQ
Sbjct: 330 HNILFAVCFNSWGGFKILFPSLMKWIGRAGLELHTKLAQEIRSAIQSTGGGKVTMAAMEQ 389

Query: 390 MPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEVICGYQPFATRDPKIF 449
           MPLMKSVVYE  RIEPPV +QYG+AKKD ++ESH+AA+++KEGE++ GYQPFAT+DPKIF
Sbjct: 390 MPLMKSVVYETLRIEPPVALQYGKAKKDFILESHEAAYQVKEGEMLFGYQPFATKDPKIF 449

Query: 450 DRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQNKQCAGKDFIVFISRLLVVELFLR 509
           DR +EFV DRF G+G +L+++V+WSNGPET++PSV NKQCAGKDF+V  +RL VVELF R
Sbjct: 450 DRPEEFVADRFVGEGVKLMEYVMWSNGPETETPSVANKQCAGKDFVVMAARLFVVELFKR 509

Query: 510 YDSFDIEASNTPLGAAVTVTSLKKASF 531
           YDSFDIE   + LGA++T+TSLK+++F
Sbjct: 510 YDSFDIEVGTSSLGASITLTSLKRSTF 536

BLAST of PI0019666 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 669.5 bits (1726), Expect = 3.2e-191
Identity = 329/519 (63.39%), Postives = 408/519 (78.61%), Query Frame = 0

Query: 15  FPSQETPQRSRSR-VGF-VSLRPIYAADGVSSSSSSSLQVPQRIVSPPEPTKLPLRKIPG 74
           FP    P+  RS+ + F V  RPI A    S S +  L V  R  S      LP+R IPG
Sbjct: 8   FPISLHPKTVRSKPLKFRVLTRPIKA----SGSETPDLTVATRTGS----KDLPIRNIPG 67

Query: 75  DYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDG 134
           +YG P+ G +KDR DYFY+QG EE+ KSR+ +Y STVYR NMPPG FI  + +VV LLDG
Sbjct: 68  NYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENPQVVALLDG 127

Query: 135 KSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQLIFFLLKHRR 194
           KSFPVLFD  KVEKKDLFTGTYMP TELTGGYR+LSY+DPSEP H KLK L+FFLLK  R
Sbjct: 128 KSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFLLKSSR 187

Query: 195 DKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFGANPVDSKLGRDA 254
           ++I PEF +T+SELF++LEK+L+  G+A++  S +  AFNFLAR+ +G NP D+KL  DA
Sbjct: 188 NRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPADTKLKADA 247

Query: 255 PKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYKSSEAVFEE 314
           P LI KWVLF L P+LS+GLP+V+EE L+ T  LPPAL+K+DY+RLY+FF +S+  +  E
Sbjct: 248 PGLITKWVLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEFFLESAGEILVE 307

Query: 315 ADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTQLAREIRTAVKAN 374
           AD+LGISREEA HNLLF TCFN++GGMKI FPNM+K IGRAG  +H +LA EIR+ +K+N
Sbjct: 308 ADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRLAEEIRSVIKSN 367

Query: 375 GGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEVICGY 434
           GG++TMGA+E+M L KSVVYE  R EPPV  QYGRAKKDLV+ESHDAAF++K GE++ GY
Sbjct: 368 GGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAFKVKAGEMLYGY 427

Query: 435 QPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPSVQNKQCAGKDFIVF 494
           QP ATRDPKIFDRADEFVP+RF G +GE+LL+HVLWSNGPET++P+V NKQCAGKDF+V 
Sbjct: 428 QPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGNKQCAGKDFVVL 487

Query: 495 ISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           ++RL V+E+F RYDSFDIE   +PLG++V  +SL+KASF
Sbjct: 488 VARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKASF 518

BLAST of PI0019666 vs. ExPASy Swiss-Prot
Match: Q40778 (Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 637.5 bits (1643), Expect = 1.3e-181
Identity = 298/471 (63.27%), Postives = 380/471 (80.68%), Query Frame = 0

Query: 61  EPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQ-GREEYLKSRMLRYESTVYRTNMPPGPF 120
           +P+  PLR+IPG YG P F  +KDR +YFY   GR+EY +SRM +Y+STV+R NMPPGPF
Sbjct: 2   DPSSKPLREIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPGPF 61

Query: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180
           ++S+ +V+VLLD KSFP+LFD SKVEKKDLFTGTYMP T+LTGGYRVLSY+DPSEP HA+
Sbjct: 62  VSSNPKVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQ 121

Query: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240
           LK L+FF+LK+  ++++P+F +T++ELFE LE +LA  G+A +N  GEQAAF FL R+ F
Sbjct: 122 LKNLLFFMLKNSSNRVIPQFETTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRAYF 181

Query: 241 GANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300
            +NP ++KLG  AP LI+ WVLF L P L LGLP  ++E LL T RLP  LIK+ Y +LY
Sbjct: 182 NSNPEETKLGTSAPTLISSWVLFNLAPTLDLGLPWFLQEPLLHTFRLPAFLIKSTYNKLY 241

Query: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360
           D+F   +  V E+A++LG+ ++EA HN+LF  CFN+FGG+KI FPN +KWIG AG NLHT
Sbjct: 242 DYFQSVATPVMEQAEKLGVPKDEAVHNILFAVCFNTFGGVKILFPNTLKWIGLAGENLHT 301

Query: 361 QLAREIRTAVKANG-GKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           QLA EIR A+K+ G G +T+ A+EQMPL KSVVYE+ RIEPPVP QYG+AK +  +ESHD
Sbjct: 302 QLAEEIRGAIKSYGDGNVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIESHD 361

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           A FE+K+GE++ GYQPFAT+DPK+FDR +E+VPDRF GDGE LLK+V WSNGPET+SP+V
Sbjct: 362 ATFEVKKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGDGEALLKYVWWSNGPETESPTV 421

Query: 481 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKAS 530
           +NKQCAGKDF+V I+RL V+ELF RYDSF+IE   +PLGAAVT+T LK+AS
Sbjct: 422 ENKQCAGKDFVVLITRLFVIELFRRYDSFEIELGESPLGAAVTLTFLKRAS 472

BLAST of PI0019666 vs. ExPASy TrEMBL
Match: A0A5A7VCB0 (Allene oxide synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G006100 PE=4 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.2e-303
Identity = 524/531 (98.68%), Postives = 530/531 (99.81%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFP-SQETPQRSRSRVGFVSLRPIYAADGVSSSSSSSLQVPQRIVSP 60
           MSSIVIPSLQPHLRFP SQETPQRSRSRVGFVS+RPIYAADGVSSSSSSSLQVPQRIVSP
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYAADGVSSSSSSSLQVPQRIVSP 60

Query: 61  PEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120
           PEPTKLPLRK+PGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 61  PEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120

Query: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180
           ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK
Sbjct: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180

Query: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240
           LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF
Sbjct: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240

Query: 241 GANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300
           GA+PVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 241 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300

Query: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360
           DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT
Sbjct: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360

Query: 361 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 420
           +LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 361 RLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 420

Query: 421 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 480
           AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSPSVQ
Sbjct: 421 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQ 480

Query: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

BLAST of PI0019666 vs. ExPASy TrEMBL
Match: A0A1S3BAD5 (allene oxide synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487935 PE=4 SV=1)

HSP 1 Score: 1046.2 bits (2704), Expect = 4.6e-302
Identity = 522/531 (98.31%), Postives = 528/531 (99.44%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFP-SQETPQRSRSRVGFVSLRPIYAADGVSSSSSSSLQVPQRIVSP 60
           MSSIVIPSLQPHLRFP SQETPQRSRSRVGFVS+RPIYA DGVSSSSSSSLQVPQRIVSP
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSP 60

Query: 61  PEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120
           PEPTKLPLRK+PGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 61  PEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120

Query: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180
           ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK
Sbjct: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180

Query: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240
           LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF
Sbjct: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240

Query: 241 GANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300
           GA+PVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 241 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300

Query: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360
           DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT
Sbjct: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360

Query: 361 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 420
           +LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 361 RLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDA 420

Query: 421 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 480
           AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSPSVQ
Sbjct: 421 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQ 480

Query: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

BLAST of PI0019666 vs. ExPASy TrEMBL
Match: Q8L5M5 (Allene oxide synthase OS=Cucumis melo OX=3656 GN=AOS PE=2 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.2e-299
Identity = 521/537 (97.02%), Postives = 527/537 (98.14%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFP-SQETPQRSRSRVGFVSLRPIYAADGVSSSSSSSLQVPQRIVSP 60
           MSSIVIPSLQPHLRFP SQETPQRSRSRVGFVS+RPIYA DGVSSSSSSSLQVPQRIVSP
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSP 60

Query: 61  PEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120
           PEPTKLPLRK+PGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 61  PEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120

Query: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180
           ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK
Sbjct: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180

Query: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240
           LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF
Sbjct: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240

Query: 241 GANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300
           GA+PVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 241 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300

Query: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360
           DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT
Sbjct: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360

Query: 361 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 420
           +LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 361 RLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDA 420

Query: 421 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 480
           AFEIKEGEVICGYQPFATRDPKIFDRADE VPDRFTG+GEELLKHV+WSNGPETQSPSVQ
Sbjct: 421 AFEIKEGEVICGYQPFATRDPKIFDRADELVPDRFTGEGEELLKHVIWSNGPETQSPSVQ 480

Query: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLG------AAVTVTSLKKASF 531
           NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLG      AAVTVTSLKKASF
Sbjct: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVSAAVTVTSLKKASF 537

BLAST of PI0019666 vs. ExPASy TrEMBL
Match: U5YTX9 (Allene oxide synthase OS=Cucumis sativus OX=3659 GN=AOS PE=2 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 6.3e-299
Identity = 519/532 (97.56%), Postives = 527/532 (99.06%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFP-SQETPQRSRSRVGFVSLRPI-YAADGVSSSSSSSLQVPQRIVS 60
           MSSIVIPSLQPHLRFP SQETPQRSRSRVGFVS+RPI YAADGVSSSSSSSL+VPQRIVS
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIVYAADGVSSSSSSSLRVPQRIVS 60

Query: 61  PPEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
           PPEP KLPLRKIPGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGP
Sbjct: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180
           FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA
Sbjct: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180

Query: 181 KLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSL 240
           KLKQL+FFLLKHRRDKIMPEFHSTFSELFETLEKDLAA+GRAEYNA GEQAAFNFLARSL
Sbjct: 181 KLKQLVFFLLKHRRDKIMPEFHSTFSELFETLEKDLAASGRAEYNAPGEQAAFNFLARSL 240

Query: 241 FGANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           FGA+PVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL
Sbjct: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300

Query: 301 YDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360
           Y+FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH
Sbjct: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360

Query: 361 TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD
Sbjct: 361 TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           AAFEIKEGE+ICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV
Sbjct: 421 AAFEIKEGEMICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480

Query: 481 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           QNKQCAGKDFIVFISRL+VVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532

BLAST of PI0019666 vs. ExPASy TrEMBL
Match: A0A6J1K6Z3 (allene oxide synthase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491740 PE=4 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 7.7e-281
Identity = 482/534 (90.26%), Postives = 508/534 (95.13%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSQETPQRSRSRVGFVSLRPIYAADGVSSSSS----SSLQVPQRI 60
           ++SI +P  QPHLRFPSQ+ P RSRS +GF S R IYAADGVSSSSS    SS+Q+PQRI
Sbjct: 3   VTSITLPCPQPHLRFPSQKKPLRSRSNLGFPSRRLIYAADGVSSSSSSSPPSSVQLPQRI 62

Query: 61  VSPPEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPP 120
              PEPTKLPLRKIPGDYGPP+FGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPP
Sbjct: 63  APTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPP 122

Query: 121 GPFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPD 180
           GPFIT+DSRVVVLLDGKSFPVLFD++KVEKKDLFTGTYMP TELTGGYRVLSYIDPSEPD
Sbjct: 123 GPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSEPD 182

Query: 181 HAKLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLAR 240
           H KLKQLIFFLLKHRRDKI+PEFHSTFSELF+TLEKDLAA GRAEYNA GEQAAFNFLAR
Sbjct: 183 HEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFLAR 242

Query: 241 SLFGANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYR 300
           SLFGANPVD+KLGRDAPKLIAKW+LFQLGPVL LGLPKVVEELLLRTVRLPP+LIKADYR
Sbjct: 243 SLFGANPVDTKLGRDAPKLIAKWILFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKADYR 302

Query: 301 RLYDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVN 360
           RLYDFFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG N
Sbjct: 303 RLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGAN 362

Query: 361 LHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVES 420
           LHTQLAREIRTAV+ANGG+ITMGAMEQMPLMKSVV+EAFRIEPPVPVQYGRAKKDLVVES
Sbjct: 363 LHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVHEAFRIEPPVPVQYGRAKKDLVVES 422

Query: 421 HDAAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSP 480
           HDAAFEIKEGE+ICG+QPFATRDPKIFDRADEFVPDRFTGDGE LLKHVLWSNGPETQSP
Sbjct: 423 HDAAFEIKEGEMICGFQPFATRDPKIFDRADEFVPDRFTGDGERLLKHVLWSNGPETQSP 482

Query: 481 SVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           +VQNKQCAGKDFIVFISRLLVVE FLRYDSFDIE +N+PLGAA+TVTSLKKASF
Sbjct: 483 TVQNKQCAGKDFIVFISRLLVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

BLAST of PI0019666 vs. NCBI nr
Match: KAA0065268.1 (allene oxide synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1050.0 bits (2714), Expect = 6.6e-303
Identity = 524/531 (98.68%), Postives = 530/531 (99.81%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFP-SQETPQRSRSRVGFVSLRPIYAADGVSSSSSSSLQVPQRIVSP 60
           MSSIVIPSLQPHLRFP SQETPQRSRSRVGFVS+RPIYAADGVSSSSSSSLQVPQRIVSP
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYAADGVSSSSSSSLQVPQRIVSP 60

Query: 61  PEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120
           PEPTKLPLRK+PGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 61  PEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120

Query: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180
           ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK
Sbjct: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180

Query: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240
           LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF
Sbjct: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240

Query: 241 GANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300
           GA+PVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 241 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300

Query: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360
           DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT
Sbjct: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360

Query: 361 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 420
           +LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 361 RLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 420

Query: 421 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 480
           AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSPSVQ
Sbjct: 421 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQ 480

Query: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

BLAST of PI0019666 vs. NCBI nr
Match: NP_001315375.1 (allene oxide synthase 1, chloroplastic [Cucumis melo] >AAM66138.1 allene oxide synthase [Cucumis melo])

HSP 1 Score: 1037.3 bits (2681), Expect = 4.5e-299
Identity = 521/537 (97.02%), Postives = 527/537 (98.14%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFP-SQETPQRSRSRVGFVSLRPIYAADGVSSSSSSSLQVPQRIVSP 60
           MSSIVIPSLQPHLRFP SQETPQRSRSRVGFVS+RPIYA DGVSSSSSSSLQVPQRIVSP
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSP 60

Query: 61  PEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120
           PEPTKLPLRK+PGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 61  PEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 120

Query: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180
           ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK
Sbjct: 121 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 180

Query: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240
           LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF
Sbjct: 181 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 240

Query: 241 GANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300
           GA+PVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 241 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 300

Query: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360
           DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT
Sbjct: 301 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 360

Query: 361 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 420
           +LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 361 RLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDA 420

Query: 421 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 480
           AFEIKEGEVICGYQPFATRDPKIFDRADE VPDRFTG+GEELLKHV+WSNGPETQSPSVQ
Sbjct: 421 AFEIKEGEVICGYQPFATRDPKIFDRADELVPDRFTGEGEELLKHVIWSNGPETQSPSVQ 480

Query: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLG------AAVTVTSLKKASF 531
           NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLG      AAVTVTSLKKASF
Sbjct: 481 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVSAAVTVTSLKKASF 537

BLAST of PI0019666 vs. NCBI nr
Match: NP_001274390.1 (allene oxide synthase [Cucumis sativus] >AGZ95026.1 allene oxide synthase [Cucumis sativus] >KGN62561.1 hypothetical protein Csa_021833 [Cucumis sativus])

HSP 1 Score: 1035.8 bits (2677), Expect = 1.3e-298
Identity = 519/532 (97.56%), Postives = 527/532 (99.06%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFP-SQETPQRSRSRVGFVSLRPI-YAADGVSSSSSSSLQVPQRIVS 60
           MSSIVIPSLQPHLRFP SQETPQRSRSRVGFVS+RPI YAADGVSSSSSSSL+VPQRIVS
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIVYAADGVSSSSSSSLRVPQRIVS 60

Query: 61  PPEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
           PPEP KLPLRKIPGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGP
Sbjct: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180
           FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA
Sbjct: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180

Query: 181 KLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSL 240
           KLKQL+FFLLKHRRDKIMPEFHSTFSELFETLEKDLAA+GRAEYNA GEQAAFNFLARSL
Sbjct: 181 KLKQLVFFLLKHRRDKIMPEFHSTFSELFETLEKDLAASGRAEYNAPGEQAAFNFLARSL 240

Query: 241 FGANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           FGA+PVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL
Sbjct: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300

Query: 301 YDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360
           Y+FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH
Sbjct: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360

Query: 361 TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD
Sbjct: 361 TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           AAFEIKEGE+ICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV
Sbjct: 421 AAFEIKEGEMICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480

Query: 481 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           QNKQCAGKDFIVFISRL+VVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532

BLAST of PI0019666 vs. NCBI nr
Match: XP_038885277.1 (allene oxide synthase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.2e-289
Identity = 503/531 (94.73%), Postives = 514/531 (96.80%), Query Frame = 0

Query: 2   SSIVIPSLQPHLRFPSQETPQRSRSRVGFVSLRPIYAADGV--SSSSSSSLQVPQRIVSP 61
           +SI IP LQP LRFP QE P RSRSRVGFVSLRPIYAAD V  SSSSSSS+QVPQR VS 
Sbjct: 4   TSITIPCLQPPLRFPYQEPPPRSRSRVGFVSLRPIYAADRVSSSSSSSSSVQVPQRTVST 63

Query: 62  PEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 121
           PEPTKLPLRKIPGDYGPP+FGALKDR DYFYNQG+EEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 64  PEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGKEEYLKSRMLRYESTVYRTNMPPGPF 123

Query: 122 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 181
           ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGG+RVLSYIDPSEPDHAK
Sbjct: 124 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGHRVLSYIDPSEPDHAK 183

Query: 182 LKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLF 241
           LKQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAAAGRA+YNA GEQAAFNFLARSLF
Sbjct: 184 LKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGRAQYNAPGEQAAFNFLARSLF 243

Query: 242 GANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 301
           GANPVDSKLGRDAPKLI KWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 244 GANPVDSKLGRDAPKLIGKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 303

Query: 302 DFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 361
           DFFY+SSEAVFEEADRLGISREEACHNLLF TCFNSFGGMKIFFPNMIKWIGRAGV LHT
Sbjct: 304 DFFYESSEAVFEEADRLGISREEACHNLLFATCFNSFGGMKIFFPNMIKWIGRAGVKLHT 363

Query: 362 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 421
           QLAREIRTAVK+NGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 364 QLAREIRTAVKSNGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 423

Query: 422 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 481
           AFEIKEGEVICGYQPFATRDPKIFDRA EFVPDRFTGDGEELLKHVLWSNGPETQSP+VQ
Sbjct: 424 AFEIKEGEVICGYQPFATRDPKIFDRAGEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQ 483

Query: 482 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           NKQCAGKDFIVFISRLLVVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 484 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 534

BLAST of PI0019666 vs. NCBI nr
Match: XP_022996510.1 (allene oxide synthase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 975.7 bits (2521), Expect = 1.6e-280
Identity = 482/534 (90.26%), Postives = 508/534 (95.13%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSQETPQRSRSRVGFVSLRPIYAADGVSSSSS----SSLQVPQRI 60
           ++SI +P  QPHLRFPSQ+ P RSRS +GF S R IYAADGVSSSSS    SS+Q+PQRI
Sbjct: 3   VTSITLPCPQPHLRFPSQKKPLRSRSNLGFPSRRLIYAADGVSSSSSSSPPSSVQLPQRI 62

Query: 61  VSPPEPTKLPLRKIPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPP 120
              PEPTKLPLRKIPGDYGPP+FGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPP
Sbjct: 63  APTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPP 122

Query: 121 GPFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPD 180
           GPFIT+DSRVVVLLDGKSFPVLFD++KVEKKDLFTGTYMP TELTGGYRVLSYIDPSEPD
Sbjct: 123 GPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSEPD 182

Query: 181 HAKLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLAR 240
           H KLKQLIFFLLKHRRDKI+PEFHSTFSELF+TLEKDLAA GRAEYNA GEQAAFNFLAR
Sbjct: 183 HEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFLAR 242

Query: 241 SLFGANPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYR 300
           SLFGANPVD+KLGRDAPKLIAKW+LFQLGPVL LGLPKVVEELLLRTVRLPP+LIKADYR
Sbjct: 243 SLFGANPVDTKLGRDAPKLIAKWILFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKADYR 302

Query: 301 RLYDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVN 360
           RLYDFFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG N
Sbjct: 303 RLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGAN 362

Query: 361 LHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVES 420
           LHTQLAREIRTAV+ANGG+ITMGAMEQMPLMKSVV+EAFRIEPPVPVQYGRAKKDLVVES
Sbjct: 363 LHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVHEAFRIEPPVPVQYGRAKKDLVVES 422

Query: 421 HDAAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSP 480
           HDAAFEIKEGE+ICG+QPFATRDPKIFDRADEFVPDRFTGDGE LLKHVLWSNGPETQSP
Sbjct: 423 HDAAFEIKEGEMICGFQPFATRDPKIFDRADEFVPDRFTGDGERLLKHVLWSNGPETQSP 482

Query: 481 SVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           +VQNKQCAGKDFIVFISRLLVVE FLRYDSFDIE +N+PLGAA+TVTSLKKASF
Sbjct: 483 TVQNKQCAGKDFIVFISRLLVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

BLAST of PI0019666 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 669.5 bits (1726), Expect = 2.3e-192
Identity = 329/519 (63.39%), Postives = 408/519 (78.61%), Query Frame = 0

Query: 15  FPSQETPQRSRSR-VGF-VSLRPIYAADGVSSSSSSSLQVPQRIVSPPEPTKLPLRKIPG 74
           FP    P+  RS+ + F V  RPI A    S S +  L V  R  S      LP+R IPG
Sbjct: 8   FPISLHPKTVRSKPLKFRVLTRPIKA----SGSETPDLTVATRTGS----KDLPIRNIPG 67

Query: 75  DYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDG 134
           +YG P+ G +KDR DYFY+QG EE+ KSR+ +Y STVYR NMPPG FI  + +VV LLDG
Sbjct: 68  NYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENPQVVALLDG 127

Query: 135 KSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQLIFFLLKHRR 194
           KSFPVLFD  KVEKKDLFTGTYMP TELTGGYR+LSY+DPSEP H KLK L+FFLLK  R
Sbjct: 128 KSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFLLKSSR 187

Query: 195 DKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFGANPVDSKLGRDA 254
           ++I PEF +T+SELF++LEK+L+  G+A++  S +  AFNFLAR+ +G NP D+KL  DA
Sbjct: 188 NRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPADTKLKADA 247

Query: 255 PKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYKSSEAVFEE 314
           P LI KWVLF L P+LS+GLP+V+EE L+ T  LPPAL+K+DY+RLY+FF +S+  +  E
Sbjct: 248 PGLITKWVLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEFFLESAGEILVE 307

Query: 315 ADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTQLAREIRTAVKAN 374
           AD+LGISREEA HNLLF TCFN++GGMKI FPNM+K IGRAG  +H +LA EIR+ +K+N
Sbjct: 308 ADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRLAEEIRSVIKSN 367

Query: 375 GGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEVICGY 434
           GG++TMGA+E+M L KSVVYE  R EPPV  QYGRAKKDLV+ESHDAAF++K GE++ GY
Sbjct: 368 GGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAFKVKAGEMLYGY 427

Query: 435 QPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPSVQNKQCAGKDFIVF 494
           QP ATRDPKIFDRADEFVP+RF G +GE+LL+HVLWSNGPET++P+V NKQCAGKDF+V 
Sbjct: 428 QPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGNKQCAGKDFVVL 487

Query: 495 ISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531
           ++RL V+E+F RYDSFDIE   +PLG++V  +SL+KASF
Sbjct: 488 VARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKASF 518

BLAST of PI0019666 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 278.9 bits (712), Expect = 8.6e-75
Identity = 144/367 (39.24%), Postives = 216/367 (58.86%), Query Frame = 0

Query: 144 VEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQLIFFLLKHRRDKIMPEFHSTF 203
           V+K+D+  G + P     GG RV  Y+D +EP HAK+K      LK      + E  S  
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 204 SELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFGAN-PVDSKLGRDAPKLIAKWVLF 263
           +  + T+E +++  G A Y    ++  F+FL  SL G +  V   +  +  K I  W+  
Sbjct: 64  NIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLAL 123

Query: 264 QLGPVLSLG-LPKVVEELLLRTVRLPPALIKADYRRLYDFFYKSS-EAVFEEADRLGISR 323
           Q+ P   LG +P+ +EE+LL T   P  LI  +Y++LY+F  +++ + +    +  G++R
Sbjct: 124 QVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFGLTR 183

Query: 324 EEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTQLAREIRTAVKANGGKITMGA 383
           +EA  NLLF   FN++GG  +F P++I  I      L  ++  E+R  V  +G  +    
Sbjct: 184 DEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVR-RVCGSGSDLNFKT 243

Query: 384 MEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEVICGYQPFATRDP 443
           + +M L+KSVVYE  R  PPVP+Q+ RA+KD  + SHDA FE+K+GE++CGYQP   RD 
Sbjct: 244 VNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRDA 303

Query: 444 KIFDRADEFVPDRFTGD-GEELLKHVLWSNGPETQSPSVQNKQCAGKDFIVFISRLLVVE 503
            +FD  +EF PDR+ G+ G ELL ++ WSNGP+T +PS  NKQCA KD +   + LLV +
Sbjct: 304 NVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVAD 363

Query: 504 LFLRYDS 507
           LFLRYD+
Sbjct: 364 LFLRYDT 369

BLAST of PI0019666 vs. TAIR 10
Match: AT3G53280.1 (cytochrome p450 71b5 )

HSP 1 Score: 55.1 bits (131), Expect = 2.0e-07
Identity = 33/96 (34.38%), Postives = 52/96 (54.17%), Query Frame = 0

Query: 360 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGR-AKKDLVVESHD 419
           +L  EIR A+  N  KIT   +E++  +K V+ EAFR+ PP P+   R    D+ ++   
Sbjct: 324 KLQEEIRAALGPNKEKITEEDLEKVEYLKMVIEEAFRLHPPAPLLLPRLTMSDINIQ--- 383

Query: 420 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRF 455
             + I +  +I        RDPK + + DEF+P+RF
Sbjct: 384 -GYSIPKNTMIQINTYTIGRDPKNWTKPDEFIPERF 415

BLAST of PI0019666 vs. TAIR 10
Match: AT1G11610.1 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 46.2 bits (108), Expect = 9.4e-05
Identity = 31/96 (32.29%), Postives = 51/96 (53.12%), Query Frame = 0

Query: 360 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGR-AKKDLVVESHD 419
           +L  EIR+ ++ +G  I    +E M  +K+V+ E FR+ PP+P+   R   +D+ V+ +D
Sbjct: 323 KLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFRVHPPLPLILPRLLTEDVKVKGYD 382

Query: 420 AAFEIKEGEVICGYQPFATRDPKIF-DRADEFVPDR 454
               I  G  +        RDP I+   A+EF P+R
Sbjct: 383 ----IAAGTEVLINAWSIHRDPAIWGPDAEEFKPER 414

BLAST of PI0019666 vs. TAIR 10
Match: AT1G11610.2 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 46.2 bits (108), Expect = 9.4e-05
Identity = 31/96 (32.29%), Postives = 51/96 (53.12%), Query Frame = 0

Query: 360 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGR-AKKDLVVESHD 419
           +L  EIR+ ++ +G  I    +E M  +K+V+ E FR+ PP+P+   R   +D+ V+ +D
Sbjct: 323 KLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFRVHPPLPLILPRLLTEDVKVKGYD 382

Query: 420 AAFEIKEGEVICGYQPFATRDPKIF-DRADEFVPDR 454
               I  G  +        RDP I+   A+EF P+R
Sbjct: 383 ----IAAGTEVLINAWSIHRDPAIWGPDAEEFKPER 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
K4BV524.4e-20966.42Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P... [more]
Q9LLB01.3e-19562.43Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P... [more]
P484171.4e-19463.31Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE... [more]
Q962423.2e-19163.39Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P... [more]
Q407781.3e-18163.27Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7VCB03.2e-30398.68Allene oxide synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3BAD54.6e-30298.31allene oxide synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487935 P... [more]
Q8L5M52.2e-29997.02Allene oxide synthase OS=Cucumis melo OX=3656 GN=AOS PE=2 SV=1[more]
U5YTX96.3e-29997.56Allene oxide synthase OS=Cucumis sativus OX=3659 GN=AOS PE=2 SV=1[more]
A0A6J1K6Z37.7e-28190.26allene oxide synthase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
KAA0065268.16.6e-30398.68allene oxide synthase 1 [Cucumis melo var. makuwa][more]
NP_001315375.14.5e-29997.02allene oxide synthase 1, chloroplastic [Cucumis melo] >AAM66138.1 allene oxide s... [more]
NP_001274390.11.3e-29897.56allene oxide synthase [Cucumis sativus] >AGZ95026.1 allene oxide synthase [Cucum... [more]
XP_038885277.13.2e-28994.73allene oxide synthase 1, chloroplastic [Benincasa hispida][more]
XP_022996510.11.6e-28090.26allene oxide synthase 1, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G42650.12.3e-19263.39allene oxide synthase [more]
AT4G15440.18.6e-7539.24hydroperoxide lyase 1 [more]
AT3G53280.12.0e-0734.38cytochrome p450 71b5 [more]
AT1G11610.19.4e-0532.29cytochrome P450, family 71, subfamily A, polypeptide 18 [more]
AT1G11610.29.4e-0532.29cytochrome P450, family 71, subfamily A, polypeptide 18 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 384..400
score: 31.0
coord: 437..455
score: 31.17
coord: 483..501
score: 21.66
IPR001128Cytochrome P450PFAMPF00067p450coord: 359..457
e-value: 4.4E-14
score: 52.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 61..530
e-value: 1.9E-116
score: 391.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 65..505
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..69
NoneNo IPR availablePANTHERPTHR24286:SF255ALLENE OXIDE SYNTHASE, CHLOROPLASTICcoord: 51..527
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 51..527

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0019666.1PI0019666.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009695 jasmonic acid biosynthetic process
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen