PI0019609 (gene) Melon (PI 482460) v1

Overview


Name	PI0019609
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	EF hand calcium-binding family protein
Location	chr07: 19411478 .. 19412531 (+)
RNA-Seq Expression	PI0019609
Synteny	PI0019609

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAGGGAGGAATCTCATTCCTTAAAGTGGATGAATAATTGAACCGCATAAGATTTTCACAACTTACTTTTTCTTCTCCTTTCACATTATTCTTTATGCACCCAAACACAAACACTCTTGGCCTACCTGCAAATAAATATATATATATATAAATACATGCCTAAGTCTATCTTGTAATACACTCATATCCCAACACACATACAAATCATGGCCCAAGCTGGATCTCTCTTGGTCGACACTGAGGCTCTTAGCCACATCTTCAACCTGATAGAAGCATTTAGGGCATTCGATGCAGACAACGACGGGTTGATCTCGCCAGCAGAGGTTGGGGGGATCATGGGCTCGCTCGGGTACAACCTGAGTGAGGAAGACGTGAACATGATGATGGAGGAAGGTGACATGGACAAAGACGGGTTGCTAAGCATGGGGGAGTTCTTAGAGATGAATGCAAAGAATATGGAAGTTGGGGAGTTGGGGAGCTATTTGAAGATTGCTTTTGAGGCTCTGAAGGCAGATGGGGATGATCTTGTGAGTGGGGAAGAGCTTTATGATGTGTTTGTGAATTTAGGGTTGGATGTTTCACTTGAGGATTCCATGGCTATTGTGGCTTCTATGGATGGGGATGGAGATGGAGCTATGTTTCTTCATGATTTCAAACTTATTGTTAATTCCCTTCGCTAAATACTTTATTGTCAATATGCCCTTTTATTTTATCTTACCTTTCTGTATTATGTATTTGGCTTTCATTTTTTTCTTTTTAAGTTTTACTTTTATATATGTATCAATAAATCATTCTACTATCACATCAATCACATACACACCCCATGTTTTATTGACTTATTTGAAGGGCTTTACTAATTTCAGTTCTATAATTATTTCTTTTCTTAGTGGGAGCGAATATTTTTATGATAAATTGTGGTGGGTGACAAATAATAAAGAATAGGATATTCAAAGTGTGATGTACATTTTTTTTCCATTTGTTGCAGACATAACATTTTTTAGTGTATAAAGTTTTTCGAGTGTATCGAGTGTATATCAGTAGTGTATTTTATGTA

mRNA sequence

TTAGGGAGGAATCTCATTCCTTAAAGTGGATGAATAATTGAACCGCATAAGATTTTCACAACTTACTTTTTCTTCTCCTTTCACATTATTCTTTATGCACCCAAACACAAACACTCTTGGCCTACCTGCAAATAAATATATATATATATAAATACATGCCTAAGTCTATCTTGTAATACACTCATATCCCAACACACATACAAATCATGGCCCAAGCTGGATCTCTCTTGGTCGACACTGAGGCTCTTAGCCACATCTTCAACCTGATAGAAGCATTTAGGGCATTCGATGCAGACAACGACGGGTTGATCTCGCCAGCAGAGGTTGGGGGGATCATGGGCTCGCTCGGGTACAACCTGAGTGAGGAAGACGTGAACATGATGATGGAGGAAGGTGACATGGACAAAGACGGGTTGCTAAGCATGGGGGAGTTCTTAGAGATGAATGCAAAGAATATGGAAGTTGGGGAGTTGGGGAGCTATTTGAAGATTGCTTTTGAGGCTCTGAAGGCAGATGGGGATGATCTTGTGAGTGGGGAAGAGCTTTATGATGTGTTTGTGAATTTAGGGTTGGATGTTTCACTTGAGGATTCCATGGCTATTGTGGCTTCTATGGATGGGGATGGAGATGGAGCTATGTTTCTTCATGATTTCAAACTTATTGTTAATTCCCTTCGCTAAATACTTTATTGTCAATATGCCCTTTTATTTTATCTTACCTTTCTGTATTATGTATTTGGCTTTCATTTTTTTCTTTTTAAGTTTTACTTTTATATATGTATCAATAAATCATTCTACTATCACATCAATCACATACACACCCCATGTTTTATTGACTTATTTGAAGGGCTTTACTAATTTCAGTTCTATAATTATTTCTTTTCTTAGTGGGAGCGAATATTTTTATGATAAATTGTGGTGGGTGACAAATAATAAAGAATAGGATATTCAAAGTGTGATGTACATTTTTTTTCCATTTGTTGCAGACATAACATTTTTTAGTGTATAAAGTTTTTCGAGTGTATCGAGTGTATATCAGTAGTGTATTTTATGTA

Coding sequence (CDS)

ATGGCCCAAGCTGGATCTCTCTTGGTCGACACTGAGGCTCTTAGCCACATCTTCAACCTGATAGAAGCATTTAGGGCATTCGATGCAGACAACGACGGGTTGATCTCGCCAGCAGAGGTTGGGGGGATCATGGGCTCGCTCGGGTACAACCTGAGTGAGGAAGACGTGAACATGATGATGGAGGAAGGTGACATGGACAAAGACGGGTTGCTAAGCATGGGGGAGTTCTTAGAGATGAATGCAAAGAATATGGAAGTTGGGGAGTTGGGGAGCTATTTGAAGATTGCTTTTGAGGCTCTGAAGGCAGATGGGGATGATCTTGTGAGTGGGGAAGAGCTTTATGATGTGTTTGTGAATTTAGGGTTGGATGTTTCACTTGAGGATTCCATGGCTATTGTGGCTTCTATGGATGGGGATGGAGATGGAGCTATGTTTCTTCATGATTTCAAACTTATTGTTAATTCCCTTCGCTAA

Protein sequence

MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSLR

Homology

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BLAST of PI0019609 vs. ExPASy Swiss-Prot
Match: Q5Z676 (Probable calcium-binding protein CML29 OS=Oryza sativa subsp. japonica OX=39947 GN=CML29 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.8e-26
Identity = 70/157 (44.59%), Postives = 103/157 (65.61%), Query Frame = 0


Query: 3   QAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEE 62
           QA  L VD EALS+I +L+EAF+AFD+DNDGL++  E+ G++ SLG +  E +V  M+  
Sbjct: 15  QAQQLSVDFEALSYISSLVEAFQAFDSDNDGLVTAPELRGLLASLGLDKPEHEVRDMLAR 74

Query: 63  GDMDKDGLLSMGEFLE-MNAKNMEVGELGSYLKIAFEALK--ADGDDLVSGEELYDVFVN 122
            D D+DG LS+ E L+ MNA  + +G LG+ L+ A  AL+  A  D ++  +EL  +   
Sbjct: 75  ADADRDGKLSVEELLDVMNAGQLGLGALGALLQSAVPALESAAGPDGVLGADELARLLSV 134

Query: 123 LGLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           +G   S+ED M I+A MDGDGDGA+ + +F+L+   L
Sbjct: 135 MG-TASVEDCMEIIACMDGDGDGAISVEEFRLMAQLL 170

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BLAST of PI0019609 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 7.4e-19
Identity = 49/139 (35.25%), Postives = 81/139 (58.27%), Query Frame = 0


Query: 15  SHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMG 74
           + I  L   F+ FD + DG IS  E+G IM SLG+ + EE++   + E D   DG ++  
Sbjct: 33  TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFE 92

Query: 75  EFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVA 134
           EF+E+N K M+  ++   LK AF     DG+  +S EEL++V  +LG + S+ +   ++ 
Sbjct: 93  EFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 152

Query: 135 SMDGDGDGAMFLHDFKLIV 154
            +D DGDG +   +FK+++
Sbjct: 153 GVDKDGDGTIDFEEFKIMM 171

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BLAST of PI0019609 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 9.0e-17
Identity = 49/139 (35.25%), Postives = 82/139 (58.99%), Query Frame = 0


Query: 17  IFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMGEF 76
           +  L E F+ FDA+ DG IS +E+  I+ SLG ++ E +V  MMEE D D DG +S+ EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 77  LEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVASM 136
           +++N K   V +    LK AF+    D +  +S  EL     ++G   ++E+S  I+ ++
Sbjct: 84  VDLNNKGASVKD----LKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 137 DGDGDGAMFLHDFKLIVNS 156
           D +GDG + + +F+ ++ S
Sbjct: 144 DKNGDGLISVEEFQTMMTS 158

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BLAST of PI0019609 vs. ExPASy Swiss-Prot
Match: A0T2M3 (Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-16
Identity = 48/139 (34.53%), Postives = 82/139 (58.99%), Query Frame = 0


Query: 17  IFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMGEF 76
           +  L E F+ FDA+ DG IS +E+  I+ S+G  + E +V  MMEE D D DG +S+ EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSMGSEVDEAEVKAMMEEADTDGDGYVSLQEF 83

Query: 77  LEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVASM 136
           +++N K   V +    LK AF+    D +  +S  EL +   ++G   ++E+S  I+ ++
Sbjct: 84  VDLNIKGATVKD----LKNAFKVFDRDCNGTISPAELCETLKSVGEPCTIEESKNIIHNV 143

Query: 137 DGDGDGAMFLHDFKLIVNS 156
           D +GDG + + +F+ ++ S
Sbjct: 144 DKNGDGLINVEEFQTMMTS 158

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BLAST of PI0019609 vs. ExPASy Swiss-Prot
Match: P02595 (Calmodulin OS=Patinopecten sp. OX=6574 PE=1 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 3.4e-16
Identity = 49/134 (36.57%), Postives = 72/134 (53.73%), Query Frame = 0


Query: 22  EAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMGEFLEMNA 81
           EAF  FD D DG I+  E+G +M SLG N +E ++  M+ E D D DG +   EFL M A
Sbjct: 15  EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMA 74

Query: 82  KNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVASMDGDGD 141
           + M+  +    ++ AF     DGD  +S  EL  V  NLG  ++ E+   ++   D DGD
Sbjct: 75  RKMKDTDSEEEIREAFRVFDKDGDGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGD 134

Query: 142 GAMFLHDFKLIVNS 156
           G +   +F  ++ S
Sbjct: 135 GQVNYEEFVTMMTS 148

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BLAST of PI0019609 vs. ExPASy TrEMBL
Match: A0A1S3B9P7 (probable calcium-binding protein CML29 OS=Cucumis melo OX=3656 GN=LOC103487564 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.4e-75
Identity = 150/156 (96.15%), Postives = 152/156 (97.44%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLIS AEVGGI+GSLGYNLSEEDV MMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD DKDGLLSMGEFLEMNAKNMEVGELGSYLKIA EALKADGDDLVSGEELYDVFVNL
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMEVGELGSYLKIALEALKADGDDLVSGEELYDVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GLDVSLEDSMAIVAS+DGDGDGAMFLHDFKLIVNSL
Sbjct: 121 GLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSL 156

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BLAST of PI0019609 vs. ExPASy TrEMBL
Match: A0A5D3D6S0 (Putative calcium-binding protein CML29 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1493G00180 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.4e-75
Identity = 150/156 (96.15%), Postives = 152/156 (97.44%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLIS AEVGGI+GSLGYNLSEEDV MMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD DKDGLLSMGEFLEMNAKNMEVGELGSYLKIA EALKADGDDLVSGEELYDVFVNL
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMEVGELGSYLKIALEALKADGDDLVSGEELYDVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GLDVSLEDSMAIVAS+DGDGDGAMFLHDFKLIVNSL
Sbjct: 121 GLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSL 156

[Close]

BLAST of PI0019609 vs. ExPASy TrEMBL
Match: A0A0A0L0M7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G376140 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.8e-75
Identity = 150/156 (96.15%), Postives = 152/156 (97.44%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLIS AEVGGIMGSLGYNLSEEDVNMMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIMGSLGYNLSEEDVNMMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD DKDGLLSMGEFLEMNAKNM+VGELGSYLKIA EALKAD DDLVSGEELYDVFVNL
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMDVGELGSYLKIALEALKADEDDLVSGEELYDVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GLDVSLEDSMAIVAS+DGDGDGAMFLHDFKLIVNSL
Sbjct: 121 GLDVSLEDSMAIVASIDGDGDGAMFLHDFKLIVNSL 156

[Close]

BLAST of PI0019609 vs. ExPASy TrEMBL
Match: A0A6J1J135 (probable calcium-binding protein CML29 OS=Cucurbita maxima OX=3661 GN=LOC111480326 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-65
Identity = 130/156 (83.33%), Postives = 146/156 (93.59%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQ  SL  +T++LSHIF+LIEAFRAFDADNDGLIS AEVGGIMGSLGYNLSE+DVN+MM
Sbjct: 1   MAQEASLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD+D+DGLLSMGEFLEM+A+++EVGE+G YLK+AFEAL ADGDDLVSGEELY+VFVNL
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGEVGRYLKVAFEALNADGDDLVSGEELYEVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GLDVSLED MAIVASMDGDGDGA+FL D KLIVNSL
Sbjct: 121 GLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of PI0019609 vs. ExPASy TrEMBL
Match: A0A6J1FSB7 (probable calcium-binding protein CML29 OS=Cucurbita moschata OX=3662 GN=LOC111446418 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.4e-65
Identity = 130/156 (83.33%), Postives = 146/156 (93.59%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQ  SL  +T++LSHIF+LIEAFRAFDADNDGLIS AEVGGIMGSLGYNLSE+DVN+MM
Sbjct: 1   MAQQPSLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD+D+DGLLSMGEFLEM+A+++EVGE+G YLK+AFEAL ADGDDLVSGEELY+VFVNL
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGEVGRYLKVAFEALNADGDDLVSGEELYEVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GLDVSLED MAIVASMDGDGDGA+FL D KLIVNSL
Sbjct: 121 GLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

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BLAST of PI0019609 vs. NCBI nr
Match: XP_008444124.2 (PREDICTED: probable calcium-binding protein CML29 [Cucumis melo] >XP_016899854.1 PREDICTED: probable calcium-binding protein CML29 [Cucumis melo] >KAA0052396.1 putative calcium-binding protein CML29 [Cucumis melo var. makuwa] >TYK19222.1 putative calcium-binding protein CML29 [Cucumis melo var. makuwa])

HSP 1 Score: 290.8 bits (743), Expect = 7.0e-75
Identity = 150/156 (96.15%), Postives = 152/156 (97.44%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLIS AEVGGI+GSLGYNLSEEDV MMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD DKDGLLSMGEFLEMNAKNMEVGELGSYLKIA EALKADGDDLVSGEELYDVFVNL
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMEVGELGSYLKIALEALKADGDDLVSGEELYDVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GLDVSLEDSMAIVAS+DGDGDGAMFLHDFKLIVNSL
Sbjct: 121 GLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSL 156

[Close]

BLAST of PI0019609 vs. NCBI nr
Match: XP_004142616.1 (probable calcium-binding protein CML29 [Cucumis sativus] >KGN54599.1 hypothetical protein Csa_012056 [Cucumis sativus])

HSP 1 Score: 290.0 bits (741), Expect = 1.2e-74
Identity = 150/156 (96.15%), Postives = 152/156 (97.44%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLIS AEVGGIMGSLGYNLSEEDVNMMM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIMGSLGYNLSEEDVNMMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD DKDGLLSMGEFLEMNAKNM+VGELGSYLKIA EALKAD DDLVSGEELYDVFVNL
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMDVGELGSYLKIALEALKADEDDLVSGEELYDVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GLDVSLEDSMAIVAS+DGDGDGAMFLHDFKLIVNSL
Sbjct: 121 GLDVSLEDSMAIVASIDGDGDGAMFLHDFKLIVNSL 156

[Close]

BLAST of PI0019609 vs. NCBI nr
Match: XP_038897482.1 (probable calcium-binding protein CML29 [Benincasa hispida])

HSP 1 Score: 279.3 bits (713), Expect = 2.1e-71
Identity = 141/156 (90.38%), Postives = 151/156 (96.79%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQAGSL  +TE LSHIFNLIEAFRAFDADNDGLIS AEVGGIMGSLGY+++E+DVNMMM
Sbjct: 1   MAQAGSLSAETETLSHIFNLIEAFRAFDADNDGLISSAEVGGIMGSLGYDMNEQDVNMMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD+D+DGLLS+GEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL
Sbjct: 61  EEGDVDRDGLLSIGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GL+VSLED MAIVASMDGDGDGA+FLHDFKLIVNSL
Sbjct: 121 GLEVSLEDCMAIVASMDGDGDGAVFLHDFKLIVNSL 156

[Close]

BLAST of PI0019609 vs. NCBI nr
Match: XP_022981068.1 (probable calcium-binding protein CML29 [Cucurbita maxima])

HSP 1 Score: 258.8 bits (660), Expect = 2.9e-65
Identity = 130/156 (83.33%), Postives = 146/156 (93.59%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQ  SL  +T++LSHIF+LIEAFRAFDADNDGLIS AEVGGIMGSLGYNLSE+DVN+MM
Sbjct: 1   MAQEASLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD+D+DGLLSMGEFLEM+A+++EVGE+G YLK+AFEAL ADGDDLVSGEELY+VFVNL
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGEVGRYLKVAFEALNADGDDLVSGEELYEVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GLDVSLED MAIVASMDGDGDGA+FL D KLIVNSL
Sbjct: 121 GLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of PI0019609 vs. NCBI nr
Match: XP_022941005.1 (probable calcium-binding protein CML29 [Cucurbita moschata])

HSP 1 Score: 258.1 bits (658), Expect = 5.0e-65
Identity = 130/156 (83.33%), Postives = 146/156 (93.59%), Query Frame = 0


Query: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMM 60
           MAQ  SL  +T++LSHIF+LIEAFRAFDADNDGLIS AEVGGIMGSLGYNLSE+DVN+MM
Sbjct: 1   MAQQPSLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDMDKDGLLSMGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNL 120
           EEGD+D+DGLLSMGEFLEM+A+++EVGE+G YLK+AFEAL ADGDDLVSGEELY+VFVNL
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGEVGRYLKVAFEALNADGDDLVSGEELYEVFVNL 120

Query: 121 GLDVSLEDSMAIVASMDGDGDGAMFLHDFKLIVNSL 157
           GLDVSLED MAIVASMDGDGDGA+FL D KLIVNSL
Sbjct: 121 GLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of PI0019609 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 95.1 bits (235), Expect = 5.2e-20
Identity = 49/139 (35.25%), Postives = 81/139 (58.27%), Query Frame = 0


Query: 15  SHIFNLIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMG 74
           + I  L   F+ FD + DG IS  E+G IM SLG+ + EE++   + E D   DG ++  
Sbjct: 33  TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFE 92

Query: 75  EFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVA 134
           EF+E+N K M+  ++   LK AF     DG+  +S EEL++V  +LG + S+ +   ++ 
Sbjct: 93  EFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 152

Query: 135 SMDGDGDGAMFLHDFKLIV 154
            +D DGDG +   +FK+++
Sbjct: 153 GVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of PI0019609 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 83.6 bits (205), Expect = 1.6e-16
Identity = 43/136 (31.62%), Postives = 78/136 (57.35%), Query Frame = 0


Query: 20  LIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMGEFLEM 79
           L + F  FD++ DG IS  E+GG+  ++G + +E ++N ++EE D D+DG +++ EF  +
Sbjct: 24  LKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFSTL 83

Query: 80  NAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVASMDGD 139
              +    E    ++ AF+    D + L+S  EL+ V   LG+  S+ED   ++  +D D
Sbjct: 84  CRSSSSAAE----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 143

Query: 140 GDGAMFLHDFKLIVNS 156
           GDG +   +F+ ++ S
Sbjct: 144 GDGNVNFEEFQKMMTS 155

[Close]

BLAST of PI0019609 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 83.6 bits (205), Expect = 1.6e-16
Identity = 43/136 (31.62%), Postives = 78/136 (57.35%), Query Frame = 0


Query: 20  LIEAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMGEFLEM 79
           L + F  FD++ DG IS  E+GG+  ++G + +E ++N ++EE D D+DG +++ EF  +
Sbjct: 24  LKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFSTL 83

Query: 80  NAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVASMDGD 139
              +    E    ++ AF+    D + L+S  EL+ V   LG+  S+ED   ++  +D D
Sbjct: 84  CRSSSSAAE----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDAD 143

Query: 140 GDGAMFLHDFKLIVNS 156
           GDG +   +F+ ++ S
Sbjct: 144 GDGNVNFEEFQKMMTS 155

[Close]

BLAST of PI0019609 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 81.6 bits (200), Expect = 6.0e-16
Identity = 48/132 (36.36%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 22  EAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMGEFLEMNA 81
           EAF  FD D DG I+  E+G +M SLG N +E ++  M+ E D D +G +   EFL + A
Sbjct: 15  EAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMA 74

Query: 82  KNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVASMDGDGD 141
           K M+  +    LK AF     D +  +S  EL  V  NLG  ++ E+   ++   D DGD
Sbjct: 75  KKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGD 134

Query: 142 GAMFLHDFKLIV 154
           G +   +F  I+
Sbjct: 135 GQINYEEFVKIM 146

[Close]

BLAST of PI0019609 vs. TAIR 10
Match: AT5G37780.1 (calmodulin 1 )

HSP 1 Score: 81.6 bits (200), Expect = 6.0e-16
Identity = 48/132 (36.36%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 22  EAFRAFDADNDGLISPAEVGGIMGSLGYNLSEEDVNMMMEEGDMDKDGLLSMGEFLEMNA 81
           EAF  FD D DG I+  E+G +M SLG N +E ++  M+ E D D +G +   EFL + A
Sbjct: 15  EAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMA 74

Query: 82  KNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVNLGLDVSLEDSMAIVASMDGDGD 141
           K M+  +    LK AF     D +  +S  EL  V  NLG  ++ E+   ++   D DGD
Sbjct: 75  KKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGD 134

Query: 142 GAMFLHDFKLIV 154
           G +   +F  I+
Sbjct: 135 GQINYEEFVKIM 146

The following BLAST results are available for this feature:

BLAST of PI0019609 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5Z676	2.8e-26	44.59	Probable calcium-binding protein CML29 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FYK2	7.4e-19	35.25	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
O64943	9.0e-17	35.25	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
A0T2M3	1.5e-16	34.53	Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1	[more]
P02595	3.4e-16	36.57	Calmodulin OS=Patinopecten sp. OX=6574 PE=1 SV=2	[more]

BLAST of PI0019609 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B9P7	3.4e-75	96.15	probable calcium-binding protein CML29 OS=Cucumis melo OX=3656 GN=LOC103487564 P...	[more]
A0A5D3D6S0	3.4e-75	96.15	Putative calcium-binding protein CML29 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0L0M7	5.8e-75	96.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G376140 PE=4 SV=1	[more]
A0A6J1J135	1.4e-65	83.33	probable calcium-binding protein CML29 OS=Cucurbita maxima OX=3661 GN=LOC1114803...	[more]
A0A6J1FSB7	2.4e-65	83.33	probable calcium-binding protein CML29 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of PI0019609 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008444124.2	7.0e-75	96.15	PREDICTED: probable calcium-binding protein CML29 [Cucumis melo] >XP_016899854.1...	[more]
XP_004142616.1	1.2e-74	96.15	probable calcium-binding protein CML29 [Cucumis sativus] >KGN54599.1 hypothetica...	[more]
XP_038897482.1	2.1e-71	90.38	probable calcium-binding protein CML29 [Benincasa hispida]	[more]
XP_022981068.1	2.9e-65	83.33	probable calcium-binding protein CML29 [Cucurbita maxima]	[more]
XP_022941005.1	5.0e-65	83.33	probable calcium-binding protein CML29 [Cucurbita moschata]	[more]

BLAST of PI0019609 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	5.2e-20	35.25	EF hand calcium-binding protein family	[more]
AT1G18210.1	1.6e-16	31.62	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	1.6e-16	31.62	Calcium-binding EF-hand family protein	[more]
AT1G66410.1	6.0e-16	36.36	calmodulin 4	[more]
AT5G37780.1	6.0e-16	36.36	calmodulin 1	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 55..83 e-value: 6.4 score: 11.3 coord: 92..120 e-value: 70.0 score: 2.9 coord: 19..47 e-value: 0.0024 score: 27.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 20..79 e-value: 2.6E-9 score: 37.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 51..86 score: 8.487999
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 15..50 score: 12.867577
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 20..79 e-value: 7.96218E-15 score: 62.9505
None	No IPR available	GENE3D	1.10.238.10		coord: 8..89 e-value: 2.5E-18 score: 67.9
None	No IPR available	GENE3D	1.10.238.10		coord: 91..155 e-value: 1.1E-7 score: 33.9
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 14..154
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 14..154
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 28..40
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 64..76
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 16..153

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0019609.1	PI0019609.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding