PI0019486 (gene) Melon (PI 482460) v1

Overview


Name	PI0019486
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	ribonuclease 3-like protein 2
Location	chr06: 26396485 .. 26398364 (+)
RNA-Seq Expression	PI0019486
Synteny	PI0019486

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATCCTCCGTTATAGCCGTTCAGACCATTCTTTCCTATAATTTTAAGAACAAGAAGCTCTTACAAGAAGCTCTCACTCACTCCTCTTGCTCTGACTCTCCCTCCTATGAGCGTCTCGAGTTTGTCGGCGATTCAGCTATTGGCCTTGCTATTACTAATTACTTTTTTCTCGTTTATCCCCACCTCCATCCAGGCCATCTCTCCTTACTTAAAGCTGCTAACGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCATGGATTGTATAACTATGTTAGGCGCAATGCAGCTCCTCTCGATGATAGGGTTTTCAAATTAACCTTCACTTTTCCGTTTGTTCCCAAAAATCTTTTTCTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTGCCCTGTCTTTATGTCTGTATTTTTTTTATTCTTCAGGTTAAAGAGTTTGTTGATTCTGTTGCCTTAGAAGGTAACTCAGTTCCTTATGGTGGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGGTGAGTTCTTAGTTACATTTGAAGTGTGATTATTATTACTTTATTAGCTAGGCCAATTAGTTTTCTTTTTCTCTTCAATCATTTGTAGATTATTAGGGGTTTGCTAGAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCTCAACCTGTAACTGTACTCTTTGAAGTTTGCCAAAAAAATGGGAAGCAGGTTGATATTAAGAATTGGAAAAAGAGTTCCAAGAACATCGCGAGTGTCTATGTTGATGGTACTTTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCGAGGCTTAATGCAGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGAGACTAGAATTAACACAGTTGTAACTATTGATGGTATCGATGAATCCTTTGAGATTGAAGGGGCAAAGCATAAGCTACATGATTTTTGTTGCAAGAAAAAGTGGCAGAAACCAATTTACAGGTATATATTGTATGATATTTGAGATGTTGCATACTTGCATTGTTTGGACTCTGAATTAGGAGGTAGTTTATATGTTGTAGTTGTGGATTTTTCATTTAAAAGCATGCACCCTAACTTATATATTTGCTTTCTAACCAAGTAGGATGCTGAATTCTTCAGTAATTTATAATGAAATGTACTTTTTTCTAAAGCTTGTCTGCTTCAATTGTAATCTTATATCTTCATCTAACTATTTTCATTCTTTCTGTTCATTGTAAACGTTGCAGCATTTTTTCTCCAATTTTAAGATTCATTTTACTGATCAATGCTCACATGTTTTAGAGAAATTAGTTTCAGCAATGACAGTGACCTTATATAAAACTTATTTTCTTTTGAATCTCCACCCTCTGTGCAATATAGTTTTACCTTATCAACTAAAGGCCTGTTTCCCTTCCTTTTCCATCTTACACATTGATTTTTTATGTGAATGAAACTCTTGGATATCTGTGCGGATTATCTCGAGTTGTTTTGTGGGTCATAAACAAAAGATAAAACTCAGAGAGATCATTTACATACCATAATAGTTATTTGACTATCTTTTTCCCTTCGCCTTGTCCTCCTTTTTCGAACGTGTTCTGGTGCTAAATACTTTATGGATTTCTTTCTTACTTGCAGTATGGAGAATAATCTAGGACCTTCACATGAAAGGAGATTTGTTTGCTCTGTTAAAATTCCCACTTGTTATGGTACGTTCTATATGATGGGAGATGAGAAGTCAAGAGTAAAGGATGCAGAGAACTCTGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCATCTCTAA

mRNA sequence

ATGGAATCCTCCGTTATAGCCGTTCAGACCATTCTTTCCTATAATTTTAAGAACAAGAAGCTCTTACAAGAAGCTCTCACTCACTCCTCTTGCTCTGACTCTCCCTCCTATGAGCGTCTCGAGTTTGTCGGCGATTCAGCTATTGGCCTTGCTATTACTAATTACTTTTTTCTCGTTTATCCCCACCTCCATCCAGGCCATCTCTCCTTACTTAAAGCTGCTAACGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTTAAAGAGTTTGTTGATTCTGTTGCCTTAGAAGGTAACTCAGTTCCTTATGGTGGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGATTATTAGGGGTTTGCTAGAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCTCAACCTGTAACTGTACTCTTTGAAGTTTGCCAAAAAAATGGGAAGCAGGTTGATATTAAGAATTGGAAAAAGAGTTCCAAGAACATCGCGAGTGTCTATGTTGATGGTACTTTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCGAGGCTTAATGCAGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGAGACTAGAATTAACACAGTTGTAACTATTGATGGTATCGATGAATCCTTTGAGATTGAAGGGGCAAAGCATAAGCTACATGATTTTTGTTGCAAGAAAAAGTGGCAGAAACCAATTTACAGTATGGAGAATAATCTAGGACCTTCACATGAAAGGAGATTTGTTTGCTCTGTTAAAATTCCCACTTGTTATGGTACGTTCTATATGATGGGAGATGAGAAGTCAAGAGTAAAGGATGCAGAGAACTCTGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCATCTCTAA

Coding sequence (CDS)

ATGGAATCCTCCGTTATAGCCGTTCAGACCATTCTTTCCTATAATTTTAAGAACAAGAAGCTCTTACAAGAAGCTCTCACTCACTCCTCTTGCTCTGACTCTCCCTCCTATGAGCGTCTCGAGTTTGTCGGCGATTCAGCTATTGGCCTTGCTATTACTAATTACTTTTTTCTCGTTTATCCCCACCTCCATCCAGGCCATCTCTCCTTACTTAAAGCTGCTAACGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTTAAAGAGTTTGTTGATTCTGTTGCCTTAGAAGGTAACTCAGTTCCTTATGGTGGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGATTATTAGGGGTTTGCTAGAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCTCAACCTGTAACTGTACTCTTTGAAGTTTGCCAAAAAAATGGGAAGCAGGTTGATATTAAGAATTGGAAAAAGAGTTCCAAGAACATCGCGAGTGTCTATGTTGATGGTACTTTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCGAGGCTTAATGCAGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGAGACTAGAATTAACACAGTTGTAACTATTGATGGTATCGATGAATCCTTTGAGATTGAAGGGGCAAAGCATAAGCTACATGATTTTTGTTGCAAGAAAAAGTGGCAGAAACCAATTTACAGTATGGAGAATAATCTAGGACCTTCACATGAAAGGAGATTTGTTTGCTCTGTTAAAATTCCCACTTGTTATGGTACGTTCTATATGATGGGAGATGAGAAGTCAAGAGTAAAGGATGCAGAGAACTCTGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCATCTCTAA

Protein sequence

MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVYPHLHPGHLSLLKAANVSTEKLARVAVKEFVDSVALEGNSVPYGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSMETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKIPTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL

Homology

[Close]

BLAST of PI0019486 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.6e-92
Identity = 186/337 (55.19%), Postives = 243/337 (72.11%), Query Frame = 0


Query: 4   SVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVYPHL 63
           S+ AV+ IL+Y F NK LL+EA+TH+SC+D PSYERLEF+GDSAIGLAI+NY +L YP L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 64  HPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGN-SVPYG 123
            P  LSLL+AANVSTEKLARV+                   VKEF ++V  E + SV YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 124 GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFE 183
           GL+KAPKVLAD+ ES+AGAVYVDVNFDLQ+LWVI RGLLEPI TL++LQ +PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 184 VCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSMET 243
           +C K+ K++DIKNWK  + +IA +Y+D   +ASG +  K+IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVFP- 298

Query: 244 RINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKIP- 303
            +  V+  D ++   ++  AK KL++ C KKKW KPIYS+E +      +RFVCS KI  
Sbjct: 299 -VEMVIDEDSVE--IQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 304 TCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
           T   T YM GDE+S++K AE+S+A  MIRAL++  +L
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRKSHYL 391

[Close]

BLAST of PI0019486 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 233.4 bits (594), Expect = 3.5e-60
Identity = 133/279 (47.67%), Postives = 181/279 (64.87%), Query Frame = 0


Query: 8   VQTILSYNFKNKKLLQEALTHSS-CSDSPSYERLEFVGDSAIGLAITNYFFLVYPHLHPG 67
           V+ +L Y F++ +LL+EALTH S   D+ SY+RLEFVGDSA+GLA +N+ +L  P L PG
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFADDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGPG 99

Query: 68  HLSLLKAANVSTEKLARVAVK-------------------EFVDSVALEG----NSVPYG 127
            LS L+AAN+STEKLARVAV+                   +F+++V  E     ++VPYG
Sbjct: 100 PLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPYG 159

Query: 128 G-LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLF 187
           G ++KAPKVLADIVE++A AVYVD  FDL+KLW + R L EPI T E   ++ QPVT+L 
Sbjct: 160 GSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAE--TIDEQPVTMLH 219

Query: 188 EVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSME 247
           E+CQK+GK    K W+K    + +V+V G  V  GSS QK IA+LNAAR+A  KL+ + +
Sbjct: 220 ELCQKHGKMAQFKTWQKGGMTVVNVFVAGELVGIGSSEQKVIAKLNAARDATRKLAGAKK 279

Query: 248 TRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIY 262
             + T V     DE  E+   K KL++ C ++ W KPI+
Sbjct: 280 QVLTTGVGNGLGDEIGELRECKQKLNEQCSRQNWPKPIF 316

[Close]

BLAST of PI0019486 vs. ExPASy Swiss-Prot
Match: Q9FKF0 (Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.2e-53
Identity = 138/384 (35.94%), Postives = 201/384 (52.34%), Query Frame = 0


Query: 3   SSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVYPH 62
           +SV AV+ IL+Y+F NK LL+EA+T      SP ++RLEF GDS + +A TNY    YP+
Sbjct: 2   NSVEAVEKILNYSFVNKTLLKEAITQK----SPLFDRLEFFGDSILEVAFTNYICHTYPN 61

Query: 63  LHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVPYG 122
           L    L  L+ ANVS EK AR+A                   VK F ++V  E + VPYG
Sbjct: 62  LKVKELRDLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYG 121

Query: 123 GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQ-PVTVLF 182
           GL+KAPK+LAD +ES+A  V++DVN+D+++LW I R LLEPIYT ++L ++P+ P   LF
Sbjct: 122 GLVKAPKILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLF 181

Query: 183 EVCQKNGKQ-----------------------VDIKNWKKSSKNIASVYVDGTFVASGSS 242
            +  K+G +                       VD+++ K  S  I S  +      S +S
Sbjct: 182 RLADKHGSESTSGIRKMTTSTKILLRNKDCLDVDLEDVKGKSFEICSTELFSLSTVSENS 241

Query: 243 MQKEIARLNA-------------AREALIKLSDSMETRINT--------------VVTID 302
           +  E+++                 +  L ++  + + +I T               + +D
Sbjct: 242 LTDEMSQEEVVIDEDSPNVEPEDVKGKLFEICYTRKLQIQTGSSGNPLTYEMTTKQMVVD 301

Query: 303 GIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVK--IPTCYGTFYM 315
                 E    + +L + C K KW +PI+S++   GP +E +FVCSVK  IP   GTF+M
Sbjct: 302 KDSLHVEPVNGRGELIEICTKNKWPRPIFSVQEEKGPKNEPKFVCSVKIEIPNIEGTFHM 361

[Close]

BLAST of PI0019486 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.8e-41
Identity = 119/330 (36.06%), Postives = 173/330 (52.42%), Query Frame = 0


Query: 11   ILSYNFKNKKLLQEALTHSS--CSDSPSYERLEFVGDSAIGLAITNYFFLVYPHLHPGHL 70
            +L   F+NK LL EA+TH+S   S    Y+RLEFVGD+ +   IT + F  Y  L PG L
Sbjct: 1544 VLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTDLPPGRL 1603

Query: 71   SLLKAANVSTEKLARVAVK-------------------EFVDSV---ALEGNSVPYG-GL 130
            + L+AA V+ E  ARVAVK                   EFV  V    L+     +G G 
Sbjct: 1604 TDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQEELLKPGFNSFGLGD 1663

Query: 131  MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFEVC 190
             KAPKVL DIVES+AGA+++D  +D   +W + + LL P+ T E L +   PV  L E C
Sbjct: 1664 CKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPETLPM--HPVRELQERC 1723

Query: 191  QKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSMETRI 250
            Q+  + ++ K  +  +     V+VDG  +    + QK++A+  AAR AL+ L +  ET  
Sbjct: 1724 QQQAEGLEYKASRAGNIATVEVFVDGVQIGVAQNPQKKMAQKLAARNALVVLKEK-ETAT 1783

Query: 251  NTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKIPTC- 310
                  DG  ++      +  L+D C +++W  P Y   N  GP+H +RFV SV++ T  
Sbjct: 1784 KKEDERDGEKKNGAQMFTRQTLNDICLRRQWPMPQYRCVNEGGPAHAKRFVYSVRVNTSD 1843

Query: 311  -YGTFYMMGDEKSRVKDAENSAASLMIRAL 314
               T   +G+    VK A++SAA L++  L
Sbjct: 1844 RGWTDECIGEPMPSVKKAKDSAAVLLLELL 1870

[Close]

BLAST of PI0019486 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 166.0 bits (419), Expect = 6.9e-40
Identity = 120/351 (34.19%), Postives = 173/351 (49.29%), Query Frame = 0


Query: 6    IAVQTILSYNFKNKKLLQEALTHSS--CSDSPSYERLEFVGDSAIGLAITNYFFLVYPHL 65
            + ++  L Y FK K LL EA+TH+S   S    Y+RLEFVGD+ +   IT + F  Y  L
Sbjct: 1560 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1619

Query: 66   HPGHLSLLKAANVSTEKLARVAVK-------------------EFVDSVALEGNSVPYG- 125
             PG L+ L+AA V+ E  ARVAVK                   EFV  V  E +   +  
Sbjct: 1620 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1679

Query: 126  ---GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTV 185
               G  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1680 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1739

Query: 186  LFEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALI----- 245
            L E CQ+  + ++ K  +  +     V++DG  V    + QK++A+  AAR AL      
Sbjct: 1740 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1799

Query: 246  KLSDSMETRINTVVTIDGIDESFEIEGAK---------HKLHDFCCKKKWQKPIYSMENN 305
            ++++S E  IN      G D+     G K           L+D C +K W  P Y     
Sbjct: 1800 EIAESKEKHINNGNA--GEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKE 1859

Query: 306  LGPSHERRFVCSVKIPTC--YGTFYMMGDEKSRVKDAENSAASLMIRALQE 316
             GP+H +RF   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1860 GGPAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1906

[Close]

BLAST of PI0019486 vs. ExPASy TrEMBL
Match: A0A5D3DKE4 (Ribonuclease 3-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00580 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.2e-153
Identity = 284/338 (84.02%), Postives = 300/338 (88.76%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           M SSVIAV+ ILSY FKNKKLL+EALTH S  DS SYERLEF+GDSAIGLA+TNYFFL Y
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
           P+LH G LSLL+AANVSTEKLARVA                   VKEFVDSVALEG+SVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+VCQ+NGKQVDIKNWK  SKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +T+I TVVTI+GIDESFEIEGAK KLHDFCC+KKWQKP YS+E  LGPSH RRFVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
           PTCYGTFY++GDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of PI0019486 vs. ExPASy TrEMBL
Match: A0A1S3BM12 (ribonuclease 3-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103491097 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.2e-153
Identity = 284/338 (84.02%), Postives = 300/338 (88.76%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           M SSVIAV+ ILSY FKNKKLL+EALTH S  DS SYERLEF+GDSAIGLA+TNYFFL Y
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
           P+LH G LSLL+AANVSTEKLARVA                   VKEFVDSVALEG+SVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+VCQ+NGKQVDIKNWK  SKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +T+I TVVTI+GIDESFEIEGAK KLHDFCC+KKWQKP YS+E  LGPSH RRFVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
           PTCYGTFY++GDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of PI0019486 vs. ExPASy TrEMBL
Match: A0A0A0L1E2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192230 PE=4 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.1e-148
Identity = 278/338 (82.25%), Postives = 296/338 (87.57%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           MESSVIA++ ILSYNF NK LL+EALTH S S S SYERLEFVGDSAIGLA+TNYFFL  
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFFLAS 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
           PHLH GHLSLL+AANVSTEKLARVA                   VKEFVDSVALEG+SVP
Sbjct: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGG+MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+VCQ+NGKQVDIK+WK  S +IASV+VDG FVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVHVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +TRI T VTIDGIDESFEIEGAK KLHD C K+KWQKP YS+E +LGPSHER FVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
            TCYGTFY++GDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of PI0019486 vs. ExPASy TrEMBL
Match: A0A6J1HKS4 (ribonuclease 3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111465061 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.7e-137
Identity = 255/338 (75.44%), Postives = 282/338 (83.43%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           MESSV+AV+ I+SYNFKNKKLL+EALTHSS  +S SY+RLEF+GDSAIGLA TNYFFL Y
Sbjct: 1   MESSVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAY 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
           P L  G LSLL+AAN+STEKLARVA                   V+EFVDSVALE NSVP
Sbjct: 61  PDLQQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGA+YVDVNFDLQ+LWVIIRGLLEPIYT E+L+VEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FEVCQKNGKQVDIKNW   SK ++SV+VDGTFVASGSS  KEIARLNAAREAL KLS +M
Sbjct: 181 FEVCQKNGKQVDIKNWSNGSKTVSSVFVDGTFVASGSSTHKEIARLNAAREALAKLSKTM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +  I T VTIDGIDESFEIEGAK KLHDFCCKKKW KP YS E + GPSH++RF CSVKI
Sbjct: 241 DINIRTAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKI 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
            T  G  YM+GDEKSRVKD+ENSAAS+MIRALQE+ +L
Sbjct: 301 STSSGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

[Close]

BLAST of PI0019486 vs. ExPASy TrEMBL
Match: A0A6J1F189 (ribonuclease 3-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111438633 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 8.5e-134
Identity = 249/338 (73.67%), Postives = 278/338 (82.25%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           MESSV+AV+ I+SYNFKNKKLL+EALTHSS  +S SY+RLEF+GDSAIGLA TNYFFL Y
Sbjct: 1   MESSVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAY 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
           P L  G LSLL+AAN+STEKLARVA                   V+EFVDSVALE NS+P
Sbjct: 61  PDLQQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSIP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGA+YVDVNFDLQ+LWVIIRGLLEPIYT E+L+VEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FEVCQKNGKQVDIKNW   SK ++SV+VDG FVASGSS  K IARLNAAREAL K+S +M
Sbjct: 181 FEVCQKNGKQVDIKNWSNGSKTVSSVFVDGIFVASGSSTHKVIARLNAAREALAKMSKTM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +  I   VTIDGIDESFEIEGAK KLHDFCCKKKW KP YS E + GPSH++RF CSVKI
Sbjct: 241 DINIRAAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKI 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
               G  YM+GDEKSRVKD+ENSAAS+MIRALQE+ +L
Sbjct: 301 SPPSGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

[Close]

BLAST of PI0019486 vs. NCBI nr
Match: XP_008449146.1 (PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo] >KAA0055862.1 ribonuclease 3-like protein 2 [Cucumis melo var. makuwa] >TYK24086.1 ribonuclease 3-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 284/338 (84.02%), Postives = 300/338 (88.76%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           M SSVIAV+ ILSY FKNKKLL+EALTH S  DS SYERLEF+GDSAIGLA+TNYFFL Y
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
           P+LH G LSLL+AANVSTEKLARVA                   VKEFVDSVALEG+SVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+VCQ+NGKQVDIKNWK  SKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +T+I TVVTI+GIDESFEIEGAK KLHDFCC+KKWQKP YS+E  LGPSH RRFVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
           PTCYGTFY++GDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of PI0019486 vs. NCBI nr
Match: XP_031738803.1 (LOW QUALITY PROTEIN: ribonuclease 3-like protein 3 [Cucumis sativus])

HSP 1 Score: 533.5 bits (1373), Expect = 1.3e-147
Identity = 277/338 (81.95%), Postives = 295/338 (87.28%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           MESSVIA++ ILSYNF NK LL+EALTH S S S SYERLEFVGDSAIGLA+TNYF   +
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFXSRF 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
            HLH GHLSLL+AANVSTEKLARVA                   VKEFVDSVALEG+SVP
Sbjct: 61  SHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGG+MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+VCQ+NGKQVDIK+WK  S +IASVYVDG FVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +TRI T VTIDGIDESFEIEGAK KLHD CCK+KWQKP YS+E +LGPSHER FVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCCKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
            TCYGTFY++GDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of PI0019486 vs. NCBI nr
Match: XP_031740595.1 (ribonuclease 3-like protein 2 [Cucumis sativus] >KAE8649376.1 hypothetical protein Csa_021577 [Cucumis sativus])

HSP 1 Score: 531.6 bits (1368), Expect = 4.8e-147
Identity = 279/338 (82.54%), Postives = 294/338 (86.98%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           MESSVIA++ ILSYNF NK LL+EALTH S S S SYERLEFVGDSAIGLAITNYFFL  
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
           PHLH GHLSLL+AANVSTEKLARVA                   VKEFVDSVALEG+SV 
Sbjct: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVALEGSSVH 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YG LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL
Sbjct: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F+VCQ+NGKQVDIK+WK  S +IASVYVDG FVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +TRI T VTIDGIDESFEIEGAK KLHD C K+KWQKP YS+E +LGPSHER FVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
            TCYGTFY++GDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

[Close]

BLAST of PI0019486 vs. NCBI nr
Match: XP_038903239.1 (ribonuclease 3-like protein 2 [Benincasa hispida])

HSP 1 Score: 506.1 bits (1302), Expect = 2.2e-139
Identity = 261/338 (77.22%), Postives = 285/338 (84.32%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           MESSV A + ILSYNFKNKKLL+EALTHSS  DS SYERLEF+GDSAIGLAI+NYFFL +
Sbjct: 1   MESSVRAAERILSYNFKNKKLLEEALTHSSYPDSASYERLEFIGDSAIGLAISNYFFLAF 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
           P LH G LSLL+AAN+STEKLARVA                   V+EFVDSVALE NSV 
Sbjct: 61  PDLHQGQLSLLRAANISTEKLARVAVRLGLYNYVRRNAAALDDKVREFVDSVALENNSVA 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGGL+KAPKVLADIVESVAGAVY+DVNFDLQ+LWVIIRGLLEPIYTLE LQVEPQPVT+L
Sbjct: 121 YGGLVKAPKVLADIVESVAGAVYIDVNFDLQRLWVIIRGLLEPIYTLESLQVEPQPVTLL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FEVCQKNGKQVDIKNWK    N+ SVYVDG+FVAS SSMQKEIA+L+AAREAL++LS+ M
Sbjct: 181 FEVCQKNGKQVDIKNWKNGPNNVVSVYVDGSFVASSSSMQKEIAKLSAAREALVELSNHM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +T   TVVTIDGIDESFEIEGAKHKLHDFCCKKKW KP YS E +LGPSHE+RFVCSV I
Sbjct: 241 DTNTKTVVTIDGIDESFEIEGAKHKLHDFCCKKKWPKPTYSTEKDLGPSHEKRFVCSVII 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
            T   T YM+GDEKSRVKDAENSAASLMIRALQER +L
Sbjct: 301 STPSDTLYMLGDEKSRVKDAENSAASLMIRALQERNYL 338

[Close]

BLAST of PI0019486 vs. NCBI nr
Match: XP_022965096.1 (ribonuclease 3-like protein 2 [Cucurbita maxima])

HSP 1 Score: 497.7 bits (1280), Expect = 7.7e-137
Identity = 255/338 (75.44%), Postives = 282/338 (83.43%), Query Frame = 0


Query: 1   MESSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVY 60
           MESSV+AV+ I+SYNFKNKKLL+EALTHSS  +S SY+RLEF+GDSAIGLA TNYFFL Y
Sbjct: 1   MESSVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAY 60

Query: 61  PHLHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVP 120
           P L  G LSLL+AAN+STEKLARVA                   V+EFVDSVALE NSVP
Sbjct: 61  PDLQQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGA+YVDVNFDLQ+LWVIIRGLLEPIYT E+L+VEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVL 180

Query: 181 FEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FEVCQKNGKQVDIKNW   SK ++SV+VDGTFVASGSS  KEIARLNAAREAL KLS +M
Sbjct: 181 FEVCQKNGKQVDIKNWSNGSKTVSSVFVDGTFVASGSSTHKEIARLNAAREALAKLSKTM 240

Query: 241 ETRINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKI 300
           +  I T VTIDGIDESFEIEGAK KLHDFCCKKKW KP YS E + GPSH++RF CSVKI
Sbjct: 241 DINIRTAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKI 300

Query: 301 PTCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
            T  G  YM+GDEKSRVKD+ENSAAS+MIRALQE+ +L
Sbjct: 301 STSSGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

[Close]

BLAST of PI0019486 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 340.9 bits (873), Expect = 1.1e-93
Identity = 186/337 (55.19%), Postives = 243/337 (72.11%), Query Frame = 0


Query: 4   SVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVYPHL 63
           S+ AV+ IL+Y F NK LL+EA+TH+SC+D PSYERLEF+GDSAIGLAI+NY +L YP L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 64  HPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGN-SVPYG 123
            P  LSLL+AANVSTEKLARV+                   VKEF ++V  E + SV YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 124 GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFE 183
           GL+KAPKVLAD+ ES+AGAVYVDVNFDLQ+LWVI RGLLEPI TL++LQ +PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 184 VCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSMET 243
           +C K+ K++DIKNWK  + +IA +Y+D   +ASG +  K+IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVFP- 298

Query: 244 RINTVVTIDGIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVKIP- 303
            +  V+  D ++   ++  AK KL++ C KKKW KPIYS+E +      +RFVCS KI  
Sbjct: 299 -VEMVIDEDSVE--IQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 304 TCYGTFYMMGDEKSRVKDAENSAASLMIRALQERKHL 320
           T   T YM GDE+S++K AE+S+A  MIRAL++  +L
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRKSHYL 391

[Close]

BLAST of PI0019486 vs. TAIR 10
Match: AT5G45150.1 (RNAse THREE-like protein 3 )

HSP 1 Score: 210.3 bits (534), Expect = 2.3e-54
Identity = 138/384 (35.94%), Postives = 201/384 (52.34%), Query Frame = 0


Query: 3   SSVIAVQTILSYNFKNKKLLQEALTHSSCSDSPSYERLEFVGDSAIGLAITNYFFLVYPH 62
           +SV AV+ IL+Y+F NK LL+EA+T      SP ++RLEF GDS + +A TNY    YP+
Sbjct: 2   NSVEAVEKILNYSFVNKTLLKEAITQK----SPLFDRLEFFGDSILEVAFTNYICHTYPN 61

Query: 63  LHPGHLSLLKAANVSTEKLARVA-------------------VKEFVDSVALEGNSVPYG 122
           L    L  L+ ANVS EK AR+A                   VK F ++V  E + VPYG
Sbjct: 62  LKVKELRDLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYG 121

Query: 123 GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQ-PVTVLF 182
           GL+KAPK+LAD +ES+A  V++DVN+D+++LW I R LLEPIYT ++L ++P+ P   LF
Sbjct: 122 GLVKAPKILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLF 181

Query: 183 EVCQKNGKQ-----------------------VDIKNWKKSSKNIASVYVDGTFVASGSS 242
            +  K+G +                       VD+++ K  S  I S  +      S +S
Sbjct: 182 RLADKHGSESTSGIRKMTTSTKILLRNKDCLDVDLEDVKGKSFEICSTELFSLSTVSENS 241

Query: 243 MQKEIARLNA-------------AREALIKLSDSMETRINT--------------VVTID 302
           +  E+++                 +  L ++  + + +I T               + +D
Sbjct: 242 LTDEMSQEEVVIDEDSPNVEPEDVKGKLFEICYTRKLQIQTGSSGNPLTYEMTTKQMVVD 301

Query: 303 GIDESFEIEGAKHKLHDFCCKKKWQKPIYSMENNLGPSHERRFVCSVK--IPTCYGTFYM 315
                 E    + +L + C K KW +PI+S++   GP +E +FVCSVK  IP   GTF+M
Sbjct: 302 KDSLHVEPVNGRGELIEICTKNKWPRPIFSVQEEKGPKNEPKFVCSVKIEIPNIEGTFHM 361

[Close]

BLAST of PI0019486 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 166.0 bits (419), Expect = 4.9e-41
Identity = 120/351 (34.19%), Postives = 173/351 (49.29%), Query Frame = 0


Query: 6    IAVQTILSYNFKNKKLLQEALTHSS--CSDSPSYERLEFVGDSAIGLAITNYFFLVYPHL 65
            + ++  L Y FK K LL EA+TH+S   S    Y+RLEFVGD+ +   IT + F  Y  L
Sbjct: 1560 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1619

Query: 66   HPGHLSLLKAANVSTEKLARVAVK-------------------EFVDSVALEGNSVPYG- 125
             PG L+ L+AA V+ E  ARVAVK                   EFV  V  E +   +  
Sbjct: 1620 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1679

Query: 126  ---GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTV 185
               G  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1680 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1739

Query: 186  LFEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALI----- 245
            L E CQ+  + ++ K  +  +     V++DG  V    + QK++A+  AAR AL      
Sbjct: 1740 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1799

Query: 246  KLSDSMETRINTVVTIDGIDESFEIEGAK---------HKLHDFCCKKKWQKPIYSMENN 305
            ++++S E  IN      G D+     G K           L+D C +K W  P Y     
Sbjct: 1800 EIAESKEKHINNGNA--GEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKE 1859

Query: 306  LGPSHERRFVCSVKIPTC--YGTFYMMGDEKSRVKDAENSAASLMIRALQE 316
             GP+H +RF   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1860 GGPAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1906

[Close]

BLAST of PI0019486 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 166.0 bits (419), Expect = 4.9e-41
Identity = 120/351 (34.19%), Postives = 173/351 (49.29%), Query Frame = 0


Query: 6    IAVQTILSYNFKNKKLLQEALTHSS--CSDSPSYERLEFVGDSAIGLAITNYFFLVYPHL 65
            + ++  L Y FK K LL EA+TH+S   S    Y+RLEFVGD+ +   IT + F  Y  L
Sbjct: 1561 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1620

Query: 66   HPGHLSLLKAANVSTEKLARVAVK-------------------EFVDSVALEGNSVPYG- 125
             PG L+ L+AA V+ E  ARVAVK                   EFV  V  E +   +  
Sbjct: 1621 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1680

Query: 126  ---GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTV 185
               G  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1681 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1740

Query: 186  LFEVCQKNGKQVDIKNWKKSSKNIASVYVDGTFVASGSSMQKEIARLNAAREALI----- 245
            L E CQ+  + ++ K  +  +     V++DG  V    + QK++A+  AAR AL      
Sbjct: 1741 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1800

Query: 246  KLSDSMETRINTVVTIDGIDESFEIEGAK---------HKLHDFCCKKKWQKPIYSMENN 305
            ++++S E  IN      G D+     G K           L+D C +K W  P Y     
Sbjct: 1801 EIAESKEKHINNGNA--GEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKE 1860

Query: 306  LGPSHERRFVCSVKIPTC--YGTFYMMGDEKSRVKDAENSAASLMIRALQE 316
             GP+H +RF   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1861 GGPAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1907

[Close]

BLAST of PI0019486 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-29
Identity = 90/231 (38.96%), Postives = 133/231 (57.58%), Query Frame = 0


Query: 7   AVQTILSYNFKNKKLLQEALTHSSCSD--SPSYERLEFVGDSAIGLAITNYFFLVYPHLH 66
           +++ IL+Y FK+K LL +A T +S  D  S SYE LE +GDS + + I   F  +YP   
Sbjct: 31  SLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELLGDSILNMGIIYDFIKLYPKEA 90

Query: 67  PGHLSLLKAANVSTEKLARVAVK-------------------EFVDSVALEGNSVPYGGL 126
           PG L+ L+A NV TEKLARVAV                    EFV+  A+E   +   GL
Sbjct: 91  PGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQILEFVE--AMEKYPLHSNGL 150

Query: 127 MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFEVC 186
           +K PKVLADIVES  GA+++D N   + +W +I+ LLEPI  L+++  +  P+T L E+C
Sbjct: 151 LKVPKVLADIVESTIGAIFMDCN-STETVWKVIKPLLEPIIHLDKM--KSHPMTELNEMC 210

Query: 187 QKNGKQVDIKN-WKKSSKNIASVYVDGTFVASG-SSMQKEIARLNAAREAL 215
           QK   ++  K+ W+++       +++  FV  G   ++KE AR  AA+ A+
Sbjct: 211 QKRNLKLTSKDTWEEN--QTYCFHIEDKFVGCGHHPVKKETARNFAAKNAI 254

The following BLAST results are available for this feature:

BLAST of PI0019486 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LTQ0	1.6e-92	55.19	Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1	[more]
Q6ATG6	3.5e-60	47.67	Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0...	[more]
Q9FKF0	3.2e-53	35.94	Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1	[more]
Q8LMR2	4.8e-41	36.06	Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]
Q9SP32	6.9e-40	34.19	Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV...	[more]

BLAST of PI0019486 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DKE4	8.2e-153	84.02	Ribonuclease 3-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BM12	8.2e-153	84.02	ribonuclease 3-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103491097 PE=4 SV=1	[more]
A0A0A0L1E2	2.1e-148	82.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192230 PE=4 SV=1	[more]
A0A6J1HKS4	3.7e-137	75.44	ribonuclease 3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111465061 PE=4 S...	[more]
A0A6J1F189	8.5e-134	73.67	ribonuclease 3-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111438633 PE=4...	[more]

BLAST of PI0019486 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008449146.1	1.7e-152	84.02	PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo] >KAA0055862.1 ribonuclea...	[more]
XP_031738803.1	1.3e-147	81.95	LOW QUALITY PROTEIN: ribonuclease 3-like protein 3 [Cucumis sativus]	[more]
XP_031740595.1	4.8e-147	82.54	ribonuclease 3-like protein 2 [Cucumis sativus] >KAE8649376.1 hypothetical prote...	[more]
XP_038903239.1	2.2e-139	77.22	ribonuclease 3-like protein 2 [Benincasa hispida]	[more]
XP_022965096.1	7.7e-137	75.44	ribonuclease 3-like protein 2 [Cucurbita maxima]	[more]

BLAST of PI0019486 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G20420.1	1.1e-93	55.19	RNAse THREE-like protein 2	[more]
AT5G45150.1	2.3e-54	35.94	RNAse THREE-like protein 3	[more]
AT1G01040.1	4.9e-41	34.19	dicer-like 1	[more]
AT1G01040.2	4.9e-41	34.19	dicer-like 1	[more]
AT4G15417.1	1.5e-29	38.96	RNAse II-like 1	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014720	Double-stranded RNA-binding domain	SMART	SM00358	DRBM_3	coord: 157..218 e-value: 0.036 score: 18.9 coord: 243..314 e-value: 1.8E-8 score: 44.1
IPR014720	Double-stranded RNA-binding domain	PFAM	PF00035	dsrm	coord: 157..217 e-value: 1.3E-5 score: 25.9
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 242..315 score: 10.314592
IPR014720	Double-stranded RNA-binding domain	PROSITE	PS50137	DS_RBD	coord: 143..219 score: 10.168934
IPR000999	Ribonuclease III domain	SMART	SM00535	riboneu5	coord: 20..151 e-value: 1.6E-32 score: 124.0
IPR000999	Ribonuclease III domain	PFAM	PF00636	Ribonuclease_3	coord: 38..126 e-value: 2.1E-15 score: 57.2
IPR000999	Ribonuclease III domain	PROSITE	PS00517	RNASE_3_1	coord: 38..46
IPR000999	Ribonuclease III domain	PROSITE	PS50142	RNASE_3_2	coord: 5..128 score: 31.003546
IPR000999	Ribonuclease III domain	CDD	cd00593	RIBOc	coord: 20..151 e-value: 5.01555E-41 score: 137.744
None	No IPR available	GENE3D	3.30.160.20		coord: 154..227 e-value: 4.4E-8 score: 35.0
None	No IPR available	PFAM	PF14709	DND1_DSRM	coord: 242..314 e-value: 2.0E-13 score: 50.4
None	No IPR available	GENE3D	3.30.160.20		coord: 240..316 e-value: 1.6E-16 score: 62.3
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 7..310
None	No IPR available	PANTHER	PTHR14950:SF49	RIBONUCLEASE 3-LIKE PROTEIN 2-RELATED	coord: 7..310
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 110..217
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 242..316
IPR036389	Ribonuclease III, endonuclease domain superfamily	GENE3D	1.10.1520.10	Ribonuclease III domain	coord: 1..153 e-value: 3.7E-40 score: 139.6
IPR036389	Ribonuclease III, endonuclease domain superfamily	SUPERFAMILY	69065	RNase III domain-like	coord: 4..146

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0019486.1	PI0019486.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0006396	RNA processing
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding