PI0019421 (gene) Melon (PI 482460) v1

Overview


Name	PI0019421
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	2-alkenal reductase (NADP(+)-dependent)-like
Location	chr02: 17968079 .. 17969485 (+)
RNA-Seq Expression	PI0019421
Synteny	PI0019421

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAGCAACAACAGCTTCATAACAATAAAACATCATATCAATGGCTTCCCCGTTGAGTCTGATCTCGAGCTCAAATCTCAACATATTGTCTCTCTACCCAGCCCGGTTCCGCCGCCATCGTCCTTAAAAATCTCTTTGCCTCCGTCGACCCTTATCAGATTAACCGTATGAAGACCCCTGACCCTCTACACGCTATTTCATCTGTCACTCCGTTTATCAATCCTGGACAAGTATCTATCTCAACTCTACTAATATGCCTCCATATATTCTTAAAATTGGAGATTAGATGCGTAAAGTTGAACATAACAAAAAAATCTATTGTTTAAACAATTTTTATAATTTTTTTTATTTATAGAAATATAGGTCGATATTTATATTTTTATAAAATATAAAATTAAGTCAATATCAATACAACGTTTTGACATGTGAGTGTTTTTTCTTTATTTTTTTTTTATTCTTCTGATCTATTTTAAGCTTTTGGATTTTGACATTTAATGTAATATTGGAACAAGGTGTGTCCTATTGCTTAATTTAGAGACCAATAATTCTTTTTTAGTTACCAGGCTTGAAATATAATATTCGGCTTTTATGGCTTTTGGGTTTGTTTGAAATAGGTAATTGTCATGGCGTGGCAGAAGTTGTGGATTCAGACGACTCTAAATTTGAAAGAGGTGACTTTATTAGGGGAATCATACATTGGGCTCAATATAGCATCGTTAAAGCTAAAGAAAGTGCTTTGGTAAAGCTAGATACTTTGGAATTTCCATTGACATATCATCTCGGAATTTTAGGTCAATTTTTAACTTAATTCTATTTTTTTAAGTAAATTATTTGATAACACCGTCACAATCTAACTAGGAGTAATACACCAATTATTATTACACACAATCACTTCGTAATGGATTCTGTTGAATAATCTCTTATTATTTAAACTTGTAAACAAACTAGATATATTTAAAAATATAGTCAAATGAGCCAACTTATTTATAAATATAGTAAAATTTTACGGTCTATATTGTGATAGACAATGATCTATCGTGATATAGACTTATAGTTAGTGACATTTTGCTATATTTGAAAATTTTCTAACAGTCTTTATTAAAATAGGTTAAAATAACCTTTTTTTTTTCTTCTTCTTCTGTATGATTAGGTATGAGTGGGCTCTCTGCCTATGCTGGTTTATTCCATGTAGGAAAGATTAAAGAAGGAGACAAAGTATTTGTCTCTGCTGCTTTCAGTTCGGTTGGAAATTTAGTTGGACAATTTGCAAAGCTACAAGGTTGCTATGTTGTTGGTTCTGTCGGTTCCGATCAAAAGGTGACATTACTAAAAGAGAAGCTCGGTTTCGACGAAGCATTCAACTATAACCAACAACAAGACTTGACATCAACCTTAGCCAAGTGA

mRNA sequence

ATGAAGAGCAACAACAGCTTCATAACAATAAAACATCATATCAATGGCTTCCCCGTTGAGTCTGATCTCGAGCTCAAATCTCAACATATTTTACCAGGCTTGAAATATAATATTCGGCTTTTATGGCTTTTGGGTTTGTTTGAAATAGGTAATTGTCATGGCGTGGCAGAAGTTGTGGATTCAGACGACTCTAAATTTGAAAGAGGTGACTTTATTAGGGGAATCATACATTGGGCTCAATATAGCATCGTTAAAGCTAAAGAAAGTGCTTTGGTAAAGCTAGATACTTTGGAATTTCCATTGACATATCATCTCGGAATTTTAGGTATGAGTGGGCTCTCTGCCTATGCTGGTTTATTCCATGTAGGAAAGATTAAAGAAGGAGACAAAGTATTTGTCTCTGCTGCTTTCAGTTCGGTTGGAAATTTAGTTGGACAATTTGCAAAGCTACAAGGTTGCTATGTGACATTACTAAAAGAGAAGCTCGGTTTCGACGAAGCATTCAACTATAACCAACAACAAGACTTGACATCAACCTTAGCCAAGTGA

Coding sequence (CDS)

ATGAAGAGCAACAACAGCTTCATAACAATAAAACATCATATCAATGGCTTCCCCGTTGAGTCTGATCTCGAGCTCAAATCTCAACATATTTTACCAGGCTTGAAATATAATATTCGGCTTTTATGGCTTTTGGGTTTGTTTGAAATAGGTAATTGTCATGGCGTGGCAGAAGTTGTGGATTCAGACGACTCTAAATTTGAAAGAGGTGACTTTATTAGGGGAATCATACATTGGGCTCAATATAGCATCGTTAAAGCTAAAGAAAGTGCTTTGGTAAAGCTAGATACTTTGGAATTTCCATTGACATATCATCTCGGAATTTTAGGTATGAGTGGGCTCTCTGCCTATGCTGGTTTATTCCATGTAGGAAAGATTAAAGAAGGAGACAAAGTATTTGTCTCTGCTGCTTTCAGTTCGGTTGGAAATTTAGTTGGACAATTTGCAAAGCTACAAGGTTGCTATGTGACATTACTAAAAGAGAAGCTCGGTTTCGACGAAGCATTCAACTATAACCAACAACAAGACTTGACATCAACCTTAGCCAAGTGA

Protein sequence

MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYNIRLLWLLGLFEIGNCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALVKLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCYVTLLKEKLGFDEAFNYNQQQDLTSTLAK

Homology

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BLAST of PI0019421 vs. ExPASy Swiss-Prot
Match: Q9SLN8 (2-alkenal reductase (NADP(+)-dependent) OS=Nicotiana tabacum OX=4097 GN=DBR PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.6e-33
Identity = 82/214 (38.32%), Postives = 125/214 (58.41%), Query Frame = 0


Query: 4   NNSFITIKHHINGFPVESDLELKSQHIL--------------------PGLKYNIRLL-- 63
           +N  + +K+++ G+P ESD+E+K+  I                     P ++  +R +  
Sbjct: 6   SNKQVILKNYVTGYPKESDMEIKNVTIKLKVPEGSNDVVVKNLYLSCDPYMRSRMRKIEG 65

Query: 64  WLLGLFEIGN---CHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALVKLDTLE 123
             +  F  G+    +GVA+V++S D KF++GD + G+  W +YSI+   ++ L K+   +
Sbjct: 66  SYVESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITPTQT-LFKIHDKD 125

Query: 124 FPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCYVT-- 183
            PL+Y+ GILGM G++AYAG   V   K+G+ VFVSAA  +VG LVGQFAK+ GCYV   
Sbjct: 126 VPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLGCYVVGS 185

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BLAST of PI0019421 vs. ExPASy Swiss-Prot
Match: Q39172 (NADPH-dependent oxidoreductase 2-alkenal reductase OS=Arabidopsis thaliana OX=3702 GN=AER PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.7e-30
Identity = 71/220 (32.27%), Postives = 115/220 (52.27%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYNIRLLWLLGLF---------EIG- 60
           M + N  + +K +++GFP ESD +  +  +   +      + +  L+          +G 
Sbjct: 1   MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYMRIRMGK 60

Query: 61  ------------------NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALV 120
                               +GV+ +++S    +++GD + GI+ W +YS++     A  
Sbjct: 61  PDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHF 120

Query: 121 KLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQG 180
           K+   + PL+Y+ G+LGM G++AYAG + V   KEG+ V+VSAA  +VG LVGQ AK+ G
Sbjct: 121 KIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMG 180

Query: 181 CYVT----------LLKEKLGFDEAFNYNQQQDLTSTLAK 183
           CYV           LLK K GFD+AFNY ++ DLT+ L +
Sbjct: 181 CYVVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKR 220

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BLAST of PI0019421 vs. ExPASy Swiss-Prot
Match: Q39173 (NADP-dependent alkenal double bond reductase P2 OS=Arabidopsis thaliana OX=3702 GN=P2 PE=2 SV=2)

HSP 1 Score: 131.0 bits (328), Expect = 1.4e-29
Identity = 70/217 (32.26%), Postives = 113/217 (52.07%), Query Frame = 0


Query: 3   SNNSFITIKHHINGFPVESDLELKSQHILPGLKYNIRLLWLLGLF---------EIGN-- 62
           + N  +  K H++GFP ESD    +  +   +    + + +  L+          +G   
Sbjct: 2   TTNKQVIFKDHVSGFPKESDFNFTTTTVELRVPEGSKSVLVKNLYLSCDPYMRSRMGKPD 61

Query: 63  ----------------CHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALVKLD 122
                            +GV+ V++S    +++GD + GI+ W +YS++        K+ 
Sbjct: 62  PSSALAQAYAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMAHMHFKIQ 121

Query: 123 TLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCYV 182
             + PL+Y+ G+LGM G++AYAG + V   K+G+ V+VSAA  +VG LVGQFAK+ GCYV
Sbjct: 122 HTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQFAKMMGCYV 181

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BLAST of PI0019421 vs. ExPASy Swiss-Prot
Match: Q6WAU0 ((+)-pulegone reductase OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-26
Identity = 61/141 (43.26%), Postives = 91/141 (64.54%), Query Frame = 0


Query: 53  HGVAEVVDSDDSKFERGDFIRGI-IHWAQYSIVKAKESALVKLDTLEFPLTYHLGILGMS 112
           +GV++V+DS    +E+G+ I G    W +Y++++   + L K+   + PL+Y++GILGM 
Sbjct: 78  YGVSKVLDSTHPSYEKGELIWGSQAGWEEYTLIQNPYN-LFKIQDKDVPLSYYVGILGMP 137

Query: 113 GLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCYVT----------LLKEK 172
           G++AYAG F +   K+G+ VFV+AA  SVG LVGQFAK+ GCYV           LLK K
Sbjct: 138 GMTAYAGFFEICSPKKGETVFVTAAAGSVGQLVGQFAKMFGCYVVGSAGSKEKVDLLKNK 197

Query: 173 LGFDEAFNYNQQQDLTSTLAK 183
            GFD+AFNY ++ D  + L +
Sbjct: 198 FGFDDAFNYKEESDYDTALKR 217

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BLAST of PI0019421 vs. ExPASy Swiss-Prot
Match: O34812 (Putative NADP-dependent oxidoreductase YfmJ OS=Bacillus subtilis (strain 168) OX=224308 GN=yfmJ PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 6.3e-22
Identity = 60/136 (44.12%), Postives = 83/136 (61.03%), Query Frame = 0


Query: 55  VAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALVKLDTLEFPLTYHLGILGMSGLS 114
           +AEVV SD +  ++GD + G + W ++S V   ESAL K+DT   P + +LGILGM+GL+
Sbjct: 76  IAEVV-SDGNHLKKGDIVIGNLSWQEFSAV--SESALRKIDTSLAPASAYLGILGMTGLT 135

Query: 115 AYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCYVT----------LLKEKLGF 174
           AY GL  +G+ KEG+ V VS A  +VG+ VGQ AK++G  V            LK++L F
Sbjct: 136 AYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLKQELQF 195

Query: 175 DEAFNYNQQQDLTSTL 181
           DEA NY    D+   L
Sbjct: 196 DEAINYKTADDIQKAL 208

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BLAST of PI0019421 vs. ExPASy TrEMBL
Match: A0A6J1CV06 (2-alkenal reductase (NADP(+)-dependent)-like OS=Momordica charantia OX=3673 GN=LOC111014596 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 6.7e-51
Identity = 118/222 (53.15%), Postives = 143/222 (64.41%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYNIRLLWLLGL------FEIG---- 60
           +++NN+FI IKH INGFP+++  +L SQ +   +K     + L  L      ++I     
Sbjct: 5   VETNNTFIAIKHLINGFPLQTHFQLNSQPLNLCVKPASGDILLKNLCVSIDPYQINRMNI 64

Query: 61  --------------------NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESA 120
                               + +GVAEVV S D  FE+GD + GIIHWAQYS+VKA ES 
Sbjct: 65  HSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGEST 124

Query: 121 LVKLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKL 180
           L KLDTL FP TYHLG+LG+SGL+AY GLF V KIKEG+KVFVSAA  SVGNLVGQFAKL
Sbjct: 125 LRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKL 184

Query: 181 QGCY----------VTLLKEKLGFDEAFNYNQQQDLTSTLAK 183
            GC+          VT LKEKLGFDEAFNY Q++DL STLAK
Sbjct: 185 HGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAK 226

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BLAST of PI0019421 vs. ExPASy TrEMBL
Match: A0A7J6DPN0 (PKS_ER domain-containing protein (Fragment) OS=Cannabis sativa OX=3483 GN=G4B88_003293 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 5.5e-45
Identity = 96/182 (52.75%), Postives = 129/182 (70.88%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYNIRLLWLLGLFEIGNCHGVAEVVD 60
           M+  N ++TIK  ING P ESD E+K   I+   +     + L  L+ I + +GVA+VV 
Sbjct: 1   MEVMNRYVTIKTQINGDPKESDFEIKCSSIVVSAQPGSNDVVLRNLYVI-DAYGVAKVVA 60

Query: 61  SDDSKFERGDFIRGIIHWAQYSIVKAKESALVKLDTLEFPLTYHLGILGMSGLSAYAGLF 120
           S + +FE+ D + G+I+W +YS++K +   L KLDT+ FPL++H+GI   SGL+AYAGLF
Sbjct: 61  SGNPEFEKDDLVVGMINWGEYSVLKGETYMLRKLDTMGFPLSHHVGIFAFSGLTAYAGLF 120

Query: 121 HVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCYVTLLKEKLGFDEAFNYNQQQDLTSTL 180
            VGK K+G+KVFVSAA  S+G+LVGQ+AK  GCYV LLKEKLGFD+AFNY  + DL STL
Sbjct: 121 EVGKPKKGEKVFVSAAAGSIGHLVGQYAKQFGCYVELLKEKLGFDDAFNYKDESDLKSTL 180

Query: 181 AK 183
            K
Sbjct: 181 KK 181

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BLAST of PI0019421 vs. ExPASy TrEMBL
Match: A0A6J1F1I6 (2-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita moschata OX=3662 GN=LOC111441259 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 5.5e-45
Identity = 103/222 (46.40%), Postives = 141/222 (63.51%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYN-----IRLLWL-LGLFEIG---- 60
           M+  N  + I+HHI+ FPVESD ELKS+ ++  +K       ++ L++ +  ++I     
Sbjct: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKAGCDDIVVKNLYVSIDPYQINRMKS 60

Query: 61  --------------------NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESA 120
                               + +GV EV+ S ++ F++GD + G++HWA+YSIVKA+ S 
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLLHWAEYSIVKAQTSL 120

Query: 121 LVKLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKL 180
           L ++D++  PLT HLGILG SGL+AYAGLF V K+KEG+ VFVSAA  SVG+L GQ AKL
Sbjct: 121 LRQIDSMGLPLTNHLGILGFSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 QGCYVT----------LLKEKLGFDEAFNYNQQQDLTSTLAK 183
            GCYV           LLKEKLGFDEAFNY Q++DLTSTL +
Sbjct: 181 HGCYVVGSAGSDEKVGLLKEKLGFDEAFNYKQERDLTSTLER 222

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BLAST of PI0019421 vs. ExPASy TrEMBL
Match: A0A6J1KLI5 (2-alkenal reductase (NADP(+)-dependent)-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495259 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 9.4e-45
Identity = 93/140 (66.43%), Postives = 112/140 (80.00%), Query Frame = 0


Query: 51  NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALVKLDTLEFPLTYHLGILGM 110
           + +GVAEVV S DS+F++ D ++G IHWA+YS+VKA+E  L KLDTL FPLTY+LGILGM
Sbjct: 24  SAYGVAEVVASSDSEFQKVDLVKGFIHWAEYSVVKAEECTLTKLDTLGFPLTYYLGILGM 83

Query: 111 SGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCY----------VTLLKE 170
           SG +AY GLF VGK+KEGDKVFVSAA  SVGNLVGQFAKL GCY          V LL+E
Sbjct: 84  SGHTAYTGLFGVGKVKEGDKVFVSAASGSVGNLVGQFAKLHGCYVVGCAGSDQKVALLEE 143

Query: 171 KLGFDEAFNYNQQQDLTSTL 181
           KLGFDEAFNY QQ++L+S++
Sbjct: 144 KLGFDEAFNYKQQKNLSSSI 163

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BLAST of PI0019421 vs. ExPASy TrEMBL
Match: A0A6J1I7M4 (2-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita maxima OX=3661 GN=LOC111470739 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 8.0e-44
Identity = 101/222 (45.50%), Postives = 142/222 (63.96%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYN-----IRLLWL-LGLFEIG---- 60
           M+  N  + I+++I+ FPVESD ELKS+ ++  +K       ++ L++ +  ++I     
Sbjct: 1   MEVMNRVVAIRNYIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKT 60

Query: 61  --------------------NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESA 120
                               + +GV EV+ S ++ F++GD + G++HWA+YSIVKA  + 
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLVHWAEYSIVKAHTNM 120

Query: 121 LVKLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKL 180
           L K+D++ FPLT HLGILG+SGL+AYAGLF V K+KEG+ VFVSAA  SVG+L GQ AKL
Sbjct: 121 LRKIDSMGFPLTNHLGILGLSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 QGCY----------VTLLKEKLGFDEAFNYNQQQDLTSTLAK 183
            GCY          V LLKEKLGFDEAFNY Q++DLTS+L +
Sbjct: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSSLER 222

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BLAST of PI0019421 vs. NCBI nr
Match: XP_038878370.1 (NADPH-dependent oxidoreductase 2-alkenal reductase-like [Benincasa hispida])

HSP 1 Score: 245.7 bits (626), Expect = 3.0e-61
Identity = 132/217 (60.83%), Postives = 152/217 (70.05%), Query Frame = 0


Query: 4   NNSFITIKHHINGFPVESDLELKSQH------------ILPGLKYNIRLLWLLGL----- 63
           NNSFITIKHHINGFPVESD ELKS              +L  L  ++    +  +     
Sbjct: 7   NNSFITIKHHINGFPVESDFELKSHPLRLSAQPGSADIVLKNLSVSVDPYQINRMKTHSP 66

Query: 64  -------------FEIGNCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALVK 123
                         ++ + HGVAEV+DSDDS+F++GDF++GIIHWAQYS+VKA ESAL+K
Sbjct: 67  SHTVSSVTPYIIPGQVIDVHGVAEVMDSDDSEFQKGDFVKGIIHWAQYSVVKAGESALMK 126

Query: 124 LDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGC 181
           LDT+EFPLTY+LGILG SGLSAYAGLF VG +KEGD VFVSAA  SVGNLVGQFAKLQGC
Sbjct: 127 LDTMEFPLTYYLGILGTSGLSAYAGLFDVGNVKEGDNVFVSAASGSVGNLVGQFAKLQGC 186

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BLAST of PI0019421 vs. NCBI nr
Match: XP_023530383.1 (2-alkenal reductase (NADP(+)-dependent)-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 219.2 bits (557), Expect = 3.0e-53
Identity = 120/216 (55.56%), Postives = 141/216 (65.28%), Query Frame = 0


Query: 5   NSFITIKHHINGFPVESDLELKSQ----HILPGLKYNIRLLWLLGLFEIG---------- 64
           N+F+TIKH I+GFP  SD ELKSQ     + PG      L   +  ++I           
Sbjct: 7   NTFVTIKHSIDGFPHASDFELKSQPLHLSVKPGHIVLKNLCVSIDPYQINRMKLHSSSHS 66

Query: 65  --------------NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALVKLDT 124
                         + +GVAEVV S DS FE+GD ++G IHWA+YS+VKA+E  L KLDT
Sbjct: 67  VSSATRFITPGQAISAYGVAEVVASSDSGFEKGDLVKGFIHWAEYSVVKAEECTLTKLDT 126

Query: 125 LEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCY-- 183
           L FPLTY+LGILGMSG +AY GLF VGK+KEGDKVFVSAA  SVGNLVGQ+AKL GCY  
Sbjct: 127 LGFPLTYYLGILGMSGHTAYTGLFEVGKVKEGDKVFVSAASGSVGNLVGQYAKLHGCYVV 186

[Close]

BLAST of PI0019421 vs. NCBI nr
Match: XP_022145088.1 (2-alkenal reductase (NADP(+)-dependent)-like [Momordica charantia])

HSP 1 Score: 210.3 bits (534), Expect = 1.4e-50
Identity = 118/222 (53.15%), Postives = 143/222 (64.41%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYNIRLLWLLGL------FEIG---- 60
           +++NN+FI IKH INGFP+++  +L SQ +   +K     + L  L      ++I     
Sbjct: 5   VETNNTFIAIKHLINGFPLQTHFQLNSQPLNLCVKPASGDILLKNLCVSIDPYQINRMNI 64

Query: 61  --------------------NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESA 120
                               + +GVAEVV S D  FE+GD + GIIHWAQYS+VKA ES 
Sbjct: 65  HSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGEST 124

Query: 121 LVKLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKL 180
           L KLDTL FP TYHLG+LG+SGL+AY GLF V KIKEG+KVFVSAA  SVGNLVGQFAKL
Sbjct: 125 LRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKL 184

Query: 181 QGCY----------VTLLKEKLGFDEAFNYNQQQDLTSTLAK 183
            GC+          VT LKEKLGFDEAFNY Q++DL STLAK
Sbjct: 185 HGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAK 226

[Close]

BLAST of PI0019421 vs. NCBI nr
Match: XP_022934032.1 (2-alkenal reductase (NADP(+)-dependent)-like [Cucurbita moschata])

HSP 1 Score: 190.7 bits (483), Expect = 1.1e-44
Identity = 103/222 (46.40%), Postives = 141/222 (63.51%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYN-----IRLLWL-LGLFEIG---- 60
           M+  N  + I+HHI+ FPVESD ELKS+ ++  +K       ++ L++ +  ++I     
Sbjct: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKAGCDDIVVKNLYVSIDPYQINRMKS 60

Query: 61  --------------------NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESA 120
                               + +GV EV+ S ++ F++GD + G++HWA+YSIVKA+ S 
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLLHWAEYSIVKAQTSL 120

Query: 121 LVKLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKL 180
           L ++D++  PLT HLGILG SGL+AYAGLF V K+KEG+ VFVSAA  SVG+L GQ AKL
Sbjct: 121 LRQIDSMGLPLTNHLGILGFSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 QGCYVT----------LLKEKLGFDEAFNYNQQQDLTSTLAK 183
            GCYV           LLKEKLGFDEAFNY Q++DLTSTL +
Sbjct: 181 HGCYVVGSAGSDEKVGLLKEKLGFDEAFNYKQERDLTSTLER 222

[Close]

BLAST of PI0019421 vs. NCBI nr
Match: KAG7021788.1 (2-alkenal reductase (NADP(+)-dependent) [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 190.7 bits (483), Expect = 1.1e-44
Identity = 103/222 (46.40%), Postives = 141/222 (63.51%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYN-----IRLLWL-LGLFEIG---- 60
           M+  N  + I+HHI+ FPVESD ELKS+ ++  +K       ++ L++ +  ++I     
Sbjct: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKAGCDDIVVKNLYVSIDPYQINRMKS 60

Query: 61  --------------------NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESA 120
                               + +GV EV+ S ++ F++GD + G++HWA+YSIVKA+ S 
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLLHWAEYSIVKAQTSL 120

Query: 121 LVKLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKL 180
           L ++D++  PLT HLGILG SGL+AYAGLF V K+KEG+ VFVSAA  SVG+L GQ AKL
Sbjct: 121 LRQIDSMGLPLTNHLGILGFSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 QGCY----------VTLLKEKLGFDEAFNYNQQQDLTSTLAK 183
            GCY          V LLKEKLGFDEAFNY Q++DLTSTL +
Sbjct: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLER 222

[Close]

BLAST of PI0019421 vs. TAIR 10
Match: AT5G17000.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 138.7 bits (348), Expect = 4.8e-33
Identity = 78/220 (35.45%), Postives = 120/220 (54.55%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHI---LPG-----LKYNIRL------------ 60
           M + N  + +K++++GFP ESD + K+  +   LPG     L  N+ L            
Sbjct: 1   MTATNKQVVLKNYVSGFPKESDFDFKTTTVEFKLPGGSNSVLVKNLYLSCDPYMRIRMGK 60

Query: 61  -----LWLLGLFEIGN---CHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALV 120
                  L   +  G     +GV+ V++S    +++GD + GI+ W +YS++        
Sbjct: 61  PDPSTAALAQAYAPGKPIFGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMTHMHF 120

Query: 121 KLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQG 180
           K+   + PL+Y+ G+LGM G++AYAG + V   KEG+ V+VSAA  +VG LVGQFAK+ G
Sbjct: 121 KIQHTDIPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQFAKMMG 180

Query: 181 CYVT----------LLKEKLGFDEAFNYNQQQDLTSTLAK 183
           CYV           LLK K GFD+AFNY ++ DL++ L +
Sbjct: 181 CYVVGSAGSTEKVDLLKTKFGFDDAFNYKEEPDLSAALKR 220

[Close]

BLAST of PI0019421 vs. TAIR 10
Match: AT1G65560.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.4e-32
Identity = 81/218 (37.16%), Postives = 125/218 (57.34%), Query Frame = 0


Query: 5   NSFITIKHHINGFPVESDLELKSQHILPGLK-------YNIRLLWL-------------- 64
           N  + +K++++G P E+D+E+K    +  LK       + ++ L+L              
Sbjct: 9   NKKVILKNYVDGIPTETDMEVKLGETIE-LKAPKGSSCFLVKNLYLSCDPYMRGRMRDFH 68

Query: 65  ---LGLFEIG---NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESAL---VKL 124
              L  F  G      G+A V+DSDD+ ++ GD + GII W +YS++++ ++     ++L
Sbjct: 69  GSYLPPFVPGQRIEGFGLARVIDSDDTNYKPGDIVSGIIGWEEYSLLRSSDNLQLRNIQL 128

Query: 125 DTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCY 183
           D  + PL+YHLG+LGM+G +AYAG   +   K+GD VFVSAA  +VG LVGQ AKL GCY
Sbjct: 129 DD-DIPLSYHLGLLGMAGFTAYAGFNEICCPKKGDSVFVSAACGAVGQLVGQLAKLHGCY 188

[Close]

BLAST of PI0019421 vs. TAIR 10
Match: AT3G59845.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 136.7 bits (343), Expect = 1.8e-32
Identity = 69/140 (49.29%), Postives = 93/140 (66.43%), Query Frame = 0


Query: 54  GVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALVKLD-TLEFPLTYHLGILGMSG 113
           GV++V+DSDD  F +GD I GI++W ++S +    +A+ K+D  +  PL+Y+ GILGM G
Sbjct: 86  GVSQVIDSDDPSFTKGDMIWGIVNWEEFSTI--NPAAIFKIDVNINVPLSYYTGILGMIG 145

Query: 114 LSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCYVT----------LLKEKL 173
           L+AYAG F +   K+GD VFVSAA  +VG LVGQFAKL GCYV           LL  K 
Sbjct: 146 LTAYAGFFEICSPKKGDTVFVSAASGAVGQLVGQFAKLMGCYVVGSAGSKQKVDLLLNKF 205

Query: 174 GFDEAFNYNQQQDLTSTLAK 183
           G+D+AFNY ++ DL S L +
Sbjct: 206 GYDDAFNYKEEPDLDSALKR 223

[Close]

BLAST of PI0019421 vs. TAIR 10
Match: AT5G16970.1 (alkenal reductase )

HSP 1 Score: 134.0 bits (336), Expect = 1.2e-31
Identity = 71/220 (32.27%), Postives = 115/220 (52.27%), Query Frame = 0


Query: 1   MKSNNSFITIKHHINGFPVESDLELKSQHILPGLKYNIRLLWLLGLF---------EIG- 60
           M + N  + +K +++GFP ESD +  +  +   +      + +  L+          +G 
Sbjct: 1   MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYMRIRMGK 60

Query: 61  ------------------NCHGVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALV 120
                               +GV+ +++S    +++GD + GI+ W +YS++     A  
Sbjct: 61  PDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHF 120

Query: 121 KLDTLEFPLTYHLGILGMSGLSAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQG 180
           K+   + PL+Y+ G+LGM G++AYAG + V   KEG+ V+VSAA  +VG LVGQ AK+ G
Sbjct: 121 KIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMG 180

Query: 181 CYVT----------LLKEKLGFDEAFNYNQQQDLTSTLAK 183
           CYV           LLK K GFD+AFNY ++ DLT+ L +
Sbjct: 181 CYVVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKR 220

[Close]

BLAST of PI0019421 vs. TAIR 10
Match: AT5G16960.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 131.7 bits (330), Expect = 5.9e-31
Identity = 63/139 (45.32%), Postives = 87/139 (62.59%), Query Frame = 0


Query: 54  GVAEVVDSDDSKFERGDFIRGIIHWAQYSIVKAKESALVKLDTLEFPLTYHLGILGMSGL 113
           GV++V+DS  S +E GD I G + W +YS++    +   K+    FPL+Y+ G+LGM G+
Sbjct: 83  GVSKVIDSGHSDYEEGDLIWGAVGWEEYSVITPIPNLHFKIHHTNFPLSYYTGLLGMPGM 142

Query: 114 SAYAGLFHVGKIKEGDKVFVSAAFSSVGNLVGQFAKLQGCYVT----------LLKEKLG 173
           +AY G + +   K+GD VFVSAA  +VG LVGQFAKL GCYV           LLK K G
Sbjct: 143 TAYVGFYEICTPKKGDTVFVSAASGAVGQLVGQFAKLMGCYVVGSAGSKEKVDLLKNKFG 202

Query: 174 FDEAFNYNQQQDLTSTLAK 183
           FD+AFNY ++ +L   L +
Sbjct: 203 FDDAFNYKEEHNLIGALKR 221

The following BLAST results are available for this feature:

BLAST of PI0019421 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SLN8	1.6e-33	38.32	2-alkenal reductase (NADP(+)-dependent) OS=Nicotiana tabacum OX=4097 GN=DBR PE=1...	[more]
Q39172	1.7e-30	32.27	NADPH-dependent oxidoreductase 2-alkenal reductase OS=Arabidopsis thaliana OX=37...	[more]
Q39173	1.4e-29	32.26	NADP-dependent alkenal double bond reductase P2 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6WAU0	1.9e-26	43.26	(+)-pulegone reductase OS=Mentha piperita OX=34256 PE=1 SV=1	[more]
O34812	6.3e-22	44.12	Putative NADP-dependent oxidoreductase YfmJ OS=Bacillus subtilis (strain 168) OX...	[more]

BLAST of PI0019421 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CV06	6.7e-51	53.15	2-alkenal reductase (NADP(+)-dependent)-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A7J6DPN0	5.5e-45	52.75	PKS_ER domain-containing protein (Fragment) OS=Cannabis sativa OX=3483 GN=G4B88_...	[more]
A0A6J1F1I6	5.5e-45	46.40	2-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1KLI5	9.4e-45	66.43	2-alkenal reductase (NADP(+)-dependent)-like isoform X1 OS=Cucurbita maxima OX=3...	[more]
A0A6J1I7M4	8.0e-44	45.50	2-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of PI0019421 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878370.1	3.0e-61	60.83	NADPH-dependent oxidoreductase 2-alkenal reductase-like [Benincasa hispida]	[more]
XP_023530383.1	3.0e-53	55.56	2-alkenal reductase (NADP(+)-dependent)-like [Cucurbita pepo subsp. pepo]	[more]
XP_022145088.1	1.4e-50	53.15	2-alkenal reductase (NADP(+)-dependent)-like [Momordica charantia]	[more]
XP_022934032.1	1.1e-44	46.40	2-alkenal reductase (NADP(+)-dependent)-like [Cucurbita moschata]	[more]
KAG7021788.1	1.1e-44	46.40	2-alkenal reductase (NADP(+)-dependent) [Cucurbita argyrosperma subsp. argyrospe...	[more]

BLAST of PI0019421 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17000.1	4.8e-33	35.45	Zinc-binding dehydrogenase family protein	[more]
AT1G65560.1	1.4e-32	37.16	Zinc-binding dehydrogenase family protein	[more]
AT3G59845.1	1.8e-32	49.29	Zinc-binding dehydrogenase family protein	[more]
AT5G16970.1	1.2e-31	32.27	alkenal reductase	[more]
AT5G16960.1	5.9e-31	45.32	Zinc-binding dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.180.10		coord: 52..106 e-value: 7.5E-43 score: 148.6
None	No IPR available	GENE3D	3.40.50.720		coord: 107..182 e-value: 7.5E-43 score: 148.6
None	No IPR available	PANTHER	PTHR43205:SF12	OS06G0602900 PROTEIN	coord: 51..181
IPR045010	Medium-chain dehydrogenase/reductase	PANTHER	PTHR43205	PROSTAGLANDIN REDUCTASE	coord: 51..181
IPR011032	GroES-like superfamily	SUPERFAMILY	50129	GroES-like	coord: 5..125
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 105..174

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0019421.1	PI0019421.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor