PI0019080 (gene) Melon (PI 482460) v1

Overview


Name	PI0019080
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	kunitz trypsin inhibitor 2
Location	chr12: 1860627 .. 1861262 (-)
RNA-Seq Expression	PI0019080
Synteny	PI0019080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAATTTGCATTACTCCCTTTTCTTTTCATTGCCGTTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCCGATGCCTCACCGGATGCCGTCCTCGACACTGACGGAAAGAAGCTCAGAGTTGGCGACAACTACTACATTCTCCCAGCATTTCAAGGCAGCGGTGGTGGCGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTCGTATCAACGTCGTCCAGGAACCTTATGAACAATCCGATGGTTCTCCTGTGACATTTTTACCAATAAACCCTAAAAAGGGCGTGGTTCGAGTTTCCACCGATTTGAACATCGAATTCGATGCGAGTACGAGGTGCGGGAAATCGACGGTGTGGAGATTAGGAACATTCAATCGATATTTGAGGCAATATTTCGTGACGATTGGGGGAACGAAAGGGAATCCGGGGCGAGAGACGACAGAAAATTGGTTCAAAATAGAGAAGTATGGCAATGGTTACAAGTTGGTGTATTGTCCAACAGTGTGTGAGCATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACACTGGGAAGAGGGCTCTTGTTTTGAGCGATACGCCATTTCCCGTTATGTTCAAGAAAGTTTATGCTTGA

mRNA sequence

ATGAAGAAATTTGCATTACTCCCTTTTCTTTTCATTGCCGTTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCCGATGCCTCACCGGATGCCGTCCTCGACACTGACGGAAAGAAGCTCAGAGTTGGCGACAACTACTACATTCTCCCAGCATTTCAAGGCAGCGGTGGTGGCGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTCGTATCAACGTCGTCCAGGAACCTTATGAACAATCCGATGGTTCTCCTGTGACATTTTTACCAATAAACCCTAAAAAGGGCGTGGTTCGAGTTTCCACCGATTTGAACATCGAATTCGATGCGAGTACGAGGTGCGGGAAATCGACGGTGTGGAGATTAGGAACATTCAATCGATATTTGAGGCAATATTTCGTGACGATTGGGGGAACGAAAGGGAATCCGGGGCGAGAGACGACAGAAAATTGGTTCAAAATAGAGAAGTATGGCAATGGTTACAAGTTGGTGTATTGTCCAACAGTGTGTGAGCATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACACTGGGAAGAGGGCTCTTGTTTTGAGCGATACGCCATTTCCCGTTATGTTCAAGAAAGTTTATGCTTGA

Coding sequence (CDS)

ATGAAGAAATTTGCATTACTCCCTTTTCTTTTCATTGCCGTTGTCATTGCCTCATCTGAGGTACGCTTCTGCAGAGCCGATGCCTCACCGGATGCCGTCCTCGACACTGACGGAAAGAAGCTCAGAGTTGGCGACAACTACTACATTCTCCCAGCATTTCAAGGCAGCGGTGGTGGCGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTCGTATCAACGTCGTCCAGGAACCTTATGAACAATCCGATGGTTCTCCTGTGACATTTTTACCAATAAACCCTAAAAAGGGCGTGGTTCGAGTTTCCACCGATTTGAACATCGAATTCGATGCGAGTACGAGGTGCGGGAAATCGACGGTGTGGAGATTAGGAACATTCAATCGATATTTGAGGCAATATTTCGTGACGATTGGGGGAACGAAAGGGAATCCGGGGCGAGAGACGACAGAAAATTGGTTCAAAATAGAGAAGTATGGCAATGGTTACAAGTTGGTGTATTGTCCAACAGTGTGTGAGCATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACACTGGGAAGAGGGCTCTTGTTTTGAGCGATACGCCATTTCCCGTTATGTTCAAGAAAGTTTATGCTTGA

Protein sequence

MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGGLAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGKSTVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVMCKDIGIFENTGKRALVLSDTPFPVMFKKVYA

Homology

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BLAST of PI0019080 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-47
Identity = 103/208 (49.52%), Postives = 127/208 (61.06%), Query Frame = 0


Query: 7   LPFLFI-AVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGGLAIGN 66
           L F F+ A++ A++      AD++P+ VLD DG+KLR G NYYI+P  +  GGG      
Sbjct: 9   LSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSAT 68

Query: 67  IRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRC--GKSTV 126
                  C   VVQ   E     P+ F P NPK+ VVRVSTDLNI F A   C    STV
Sbjct: 69  TPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTV 128

Query: 127 WRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKY-GNG-YKLVYCPTVCEHCKVMC 186
           WRL  ++    QYFVTIGG KGNPG ET  +WFKIE++ G+G YKLV+CPTVC  CKV C
Sbjct: 129 WRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKC 188

Query: 187 KDIGIF-ENTGKRALVLSDTPFPVMFKK 209
            D+GI+ +  G+R L LSD PF   F K
Sbjct: 189 GDVGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of PI0019080 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.3e-44
Identity = 88/202 (43.56%), Postives = 127/202 (62.87%), Query Frame = 0


Query: 9   FLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGGLAIGNIRK 68
           FL +AV I+    R    +A+ + V D +GK L  G NYYILP  +G  GGGL + N++ 
Sbjct: 8   FLLLAVFISH---RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGR-GGGLTMSNLKT 67

Query: 69  EYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGKSTVWRLGT 128
           E   C  +V+Q+ +E S G PV F P + K   + VSTD+NI+F        +++W L  
Sbjct: 68  E--TCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELAN 127

Query: 129 FNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVMCKDIGIFE 188
           F+   +Q+F++  G +GNPG++T +NWFKI+K+   YK+ +CPTVC  CKV+C+D+G+F 
Sbjct: 128 FDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFV 187

Query: 189 NTGKRALVLSDTPFPVMFKKVY 211
             GKR L LSD P  VMFK+ Y
Sbjct: 188 QDGKRRLALSDVPLKVMFKRAY 196

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BLAST of PI0019080 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.6e-37
Identity = 87/203 (42.86%), Postives = 116/203 (57.14%), Query Frame = 0


Query: 10  LFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGGLAIGNIRKE 69
           + +     S    F  A+A+   VLDTDG +L+ G  YY+L +  G+GGGGLA+G  R  
Sbjct: 8   VLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALG--RAT 67

Query: 70  YDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEF--DASTRCGKSTVWRLG 129
              C   VVQ   +  +G+PV F   + K  VVRVSTD+NIEF       C  STVWRL 
Sbjct: 68  GQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLD 127

Query: 130 TFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYG-NGYKLVYCPTVCEHCKVMCKDIGI 189
            ++    +++VT  G KG PG  T  +WFKIEK G  GYK  +CP+VC+ C  +C DIG 
Sbjct: 128 NYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGR 187

Query: 190 F-ENTGKRALVLSDTPFPVMFKK 209
             ++ G+  L LSD  +  MFKK
Sbjct: 188 HSDDDGQIRLALSDNEWAWMFKK 208

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BLAST of PI0019080 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.6e-29
Identity = 75/180 (41.67%), Postives = 102/180 (56.67%), Query Frame = 0


Query: 32  AVLDTDGKKLRVGDNYYILPAFQGSGGGGLAIGNIRKEYDRCRINVVQEPYEQSDGSPVT 91
           AV+D DG  +   ++YY+LP  +G GGG    G   +    C  ++VQE  E  +G PV 
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG---RGGQPCPYDIVQESSEVDEGIPVK 88

Query: 92  FLPINPKKGVVRVSTDLNIEFD-ASTRCGKSTVWRLGTFNRYLRQYFVTIGGTKGNPGRE 151
           F     K   V  S +LNIE D  +T C +ST WR+G F+   +QYFV  G      G++
Sbjct: 89  FSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQD 148

Query: 152 TTENWFKIEKYG-NGYKLVYCPTVCEHCKVMCKDIGIF-ENTGKRALVLSDTPFPVMFKK 209
           + +++FKIEK G + YK V+CP  C+     C D+GIF +  G R L LSD PF VMFKK
Sbjct: 149 SLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

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BLAST of PI0019080 vs. ExPASy Swiss-Prot
Match: Q9FX28 (Kunitz trypsin inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=KTI3 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.1e-24
Identity = 74/190 (38.95%), Postives = 105/190 (55.26%), Query Frame = 0


Query: 26  ADASP-DAVLDTDGKKLRVGDNYYILPAFQGSGGGGLAIGNIRKEYDRC-RINVVQEPYE 85
           ADA+P   VLD  G  ++    YYI+PA  G+GGG +         D C  +++VQ    
Sbjct: 24  ADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLNLDIVQSSSP 83

Query: 86  QSDGSPVTFLPINPKKGVVRVSTDLNIEFDAST-RCGKSTVWRLGTFNRYLRQYFVTIGG 145
              G PVTF P+N K   V++S  LN+EFD++   C  S VWR+   +  LR+ FV+IGG
Sbjct: 84  FVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRI-DHSVQLRKSFVSIGG 143

Query: 146 TKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVMCKDIGI-FENTGKRALVLS-DT 205
            KG        +WF+I++ G+ YKL+YCP       V C ++ +  ++ G R LVLS D 
Sbjct: 144 QKGK-----GNSWFQIQEDGDAYKLMYCPI---SSIVACINVSLEIDDHGVRRLVLSTDQ 203

Query: 206 PFPVMFKKVY 211
            F V F+K Y
Sbjct: 204 SFVVKFQKAY 204

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BLAST of PI0019080 vs. ExPASy TrEMBL
Match: A0A5D3BJ25 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002780 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.1e-105
Identity = 187/211 (88.63%), Postives = 197/211 (93.36%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           MKKFALL FLFIA+VIASSE+RFCRADASPDAVLDTDGKKLRV + YYILPAF+GSGGGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           LAIGNIRKEYDRC INVVQE YEQSDG P TFLPINPKKGVVRVSTDLNIEFDA+TRC K
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNG-YKLVYCPTVCEHCKV 180
           STVW+LGTF+RYLRQYFVTIGGTKGNPGRETTENWFKIEKYG G YKLVYCP VC++CKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDIGIFENTGKRALVLSDTPFPVMFKKVY 211
           MCKDIGIFEN G R LVLSDTPFPV+FKKVY
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVY 211

[Close]

BLAST of PI0019080 vs. ExPASy TrEMBL
Match: A0A1S3AT47 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.1e-105
Identity = 187/211 (88.63%), Postives = 197/211 (93.36%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           MKKFALL FLFIA+VIASSE+RFCRADASPDAVLDTDGKKLRV + YYILPAF+GSGGGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           LAIGNIRKEYDRC INVVQE YEQSDG P TFLPINPKKGVVRVSTDLNIEFDA+TRC K
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNG-YKLVYCPTVCEHCKV 180
           STVW+LGTF+RYLRQYFVTIGGTKGNPGRETTENWFKIEKYG G YKLVYCP VC++CKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDIGIFENTGKRALVLSDTPFPVMFKKVY 211
           MCKDIGIFEN G R LVLSDTPFPV+FKKVY
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVY 211

[Close]

BLAST of PI0019080 vs. ExPASy TrEMBL
Match: A0A5D3BJ02 (Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.3e-81
Identity = 147/209 (70.33%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           MK FALL FLFI  VIASSEVRFCRADASPDAVLDTDGKKLR GD YYIL  +     GG
Sbjct: 1   MKNFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           L+IG I   Y++C IN++ E Y+  DG P TF P+NPKKGVVRVSTDLNIEF+ASTRCG 
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGI 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVM 180
           STVW++G F++YL+QYFVT+GGTKGNPGRET  NWFK+EK+G  YK VYCPTVC++CKVM
Sbjct: 121 STVWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVM 180

Query: 181 CKDIGIFENTGKRALVLSDTPFPVMFKKV 210
           CKD+G+F   G+R   L+D PFPVMFKKV
Sbjct: 181 CKDVGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of PI0019080 vs. ExPASy TrEMBL
Match: A0A1S3AST3 (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.3e-81
Identity = 147/209 (70.33%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           MK FALL FLFI  VIASSEVRFCRADASPDAVLDTDGKKLR GD YYIL  +     GG
Sbjct: 1   MKNFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           L+IG I   Y++C IN++ E Y+  DG P TF P+NPKKGVVRVSTDLNIEF+ASTRCG 
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGI 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVM 180
           STVW++G F++YL+QYFVT+GGTKGNPGRET  NWFK+EK+G  YK VYCPTVC++CKVM
Sbjct: 121 STVWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVM 180

Query: 181 CKDIGIFENTGKRALVLSDTPFPVMFKKV 210
           CKD+G+F   G+R   L+D PFPVMFKKV
Sbjct: 181 CKDVGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of PI0019080 vs. ExPASy TrEMBL
Match: A0A0A0LT81 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 2.1e-80
Identity = 145/209 (69.38%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           M+ FALL FLFI  VIASSEVRFCRADASPDAVLDTDGKKLR GD YYIL  +     GG
Sbjct: 1   MRNFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           L+IG I   Y++C IN++ E Y+   G P TF PINPKKGVVRVSTDLNI+F+A+TRCG 
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGI 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVM 180
           STVW++G F+ YL+QYFVT+GG KGNPGRET ENWFK+EKYG  YKLVYCPTVC++CKV+
Sbjct: 121 STVWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVV 180

Query: 181 CKDIGIFENTGKRALVLSDTPFPVMFKKV 210
           CKD+G+F   G+R + L+D PFPVMFKKV
Sbjct: 181 CKDVGLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]

BLAST of PI0019080 vs. NCBI nr
Match: XP_008437071.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 392.5 bits (1007), Expect = 2.3e-105
Identity = 187/211 (88.63%), Postives = 197/211 (93.36%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           MKKFALL FLFIA+VIASSE+RFCRADASPDAVLDTDGKKLRV + YYILPAF+GSGGGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           LAIGNIRKEYDRC INVVQE YEQSDG P TFLPINPKKGVVRVSTDLNIEFDA+TRC K
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNG-YKLVYCPTVCEHCKV 180
           STVW+LGTF+RYLRQYFVTIGGTKGNPGRETTENWFKIEKYG G YKLVYCP VC++CKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDIGIFENTGKRALVLSDTPFPVMFKKVY 211
           MCKDIGIFEN G R LVLSDTPFPV+FKKVY
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVY 211

[Close]

BLAST of PI0019080 vs. NCBI nr
Match: XP_038895396.1 (miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa hispida])

HSP 1 Score: 312.8 bits (800), Expect = 2.3e-81
Identity = 147/209 (70.33%), Postives = 171/209 (81.82%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           MK FALL FLFI  VI SSEVRFCRAD SPDAVLDTDGKKLR  D YYIL  +     GG
Sbjct: 1   MKNFALLSFLFI--VIVSSEVRFCRADVSPDAVLDTDGKKLRSSDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           L+IG+I++  ++C IN++ E YE   G   TF PINPKKGVVRVSTDLNI+F+ASTRCG+
Sbjct: 61  LSIGSIQQGKEKCPINILPESYEYLHGLAATFFPINPKKGVVRVSTDLNIQFEASTRCGE 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVM 180
           STVW++G F++YL+QYFVTIGG KGNPGRET  NWFKIEKYGN YKLV+CPTVC++CKV+
Sbjct: 121 STVWKIGKFDQYLKQYFVTIGGVKGNPGRETVGNWFKIEKYGNNYKLVHCPTVCKYCKVI 180

Query: 181 CKDIGIFENTGKRALVLSDTPFPVMFKKV 210
           CKD+GIF   GKR L L+D+PFPVMFKKV
Sbjct: 181 CKDVGIFYKNGKRVLALNDSPFPVMFKKV 206

[Close]

BLAST of PI0019080 vs. NCBI nr
Match: XP_008437082.1 (PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. makuwa])

HSP 1 Score: 311.2 bits (796), Expect = 6.7e-81
Identity = 147/209 (70.33%), Postives = 170/209 (81.34%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           MK FALL FLFI  VIASSEVRFCRADASPDAVLDTDGKKLR GD YYIL  +     GG
Sbjct: 1   MKNFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           L+IG I   Y++C IN++ E Y+  DG P TF P+NPKKGVVRVSTDLNIEF+ASTRCG 
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGI 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVM 180
           STVW++G F++YL+QYFVT+GGTKGNPGRET  NWFK+EK+G  YK VYCPTVC++CKVM
Sbjct: 121 STVWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVM 180

Query: 181 CKDIGIFENTGKRALVLSDTPFPVMFKKV 210
           CKD+G+F   G+R   L+D PFPVMFKKV
Sbjct: 181 CKDVGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of PI0019080 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima] >KAG6584289.1 Kunitz trypsin inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019882.1 Kunitz trypsin inhibitor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 291.6 bits (745), Expect = 5.5e-75
Identity = 138/209 (66.03%), Postives = 166/209 (79.43%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           MK FALL FLFI   + +SEVR  RADASPDAV D DGKKLR G NYYILP F+G  GGG
Sbjct: 1   MKNFALLSFLFI---LLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGR-GGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           LA+GN++   D+C +NVVQE +E  +G P  FLP+NPKKGVVRVSTDLN++F+AST C  
Sbjct: 61  LALGNLQS--DKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICAT 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVM 180
           STVW+L  F+   +Q+F+TIGGT+GNPG +T +NWFKIEK+GN YK  +CPTVC+ CKVM
Sbjct: 121 STVWKLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVM 180

Query: 181 CKDIGIFENTGKRALVLSDTPFPVMFKKV 210
           C+D+GIF   GKRAL LSDTPFPVMFK+V
Sbjct: 181 CRDVGIFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of PI0019080 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 291.6 bits (745), Expect = 5.5e-75
Identity = 139/209 (66.51%), Postives = 166/209 (79.43%), Query Frame = 0


Query: 1   MKKFALLPFLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGG 60
           MK FALL FLF   +IAS+EVR C++DASPDAVLD DGKKLR G NYYILP  +G  GGG
Sbjct: 1   MKNFALLCFLF--ALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGR-GGG 60

Query: 61  LAIGNIRKEYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGK 120
           L +GNI   +D+C ++VVQE +E S+G P TF P+NPKKGVVRVSTDLN+EF+AST C K
Sbjct: 61  LTLGNI--GHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAK 120

Query: 121 STVWRLGTFNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVM 180
           STVW+L  F+   RQ+ V IGG +GNPG+ET +NWFKIEK+G  YK V+CP+VC+ CKV+
Sbjct: 121 STVWKLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVI 180

Query: 181 CKDIGIFENTGKRALVLSDTPFPVMFKKV 210
           CKD+GIF   G RAL LSDTPFPVMFKKV
Sbjct: 181 CKDVGIFIKNGNRALALSDTPFPVMFKKV 204

[Close]

BLAST of PI0019080 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 180.3 bits (456), Expect = 1.7e-45
Identity = 88/202 (43.56%), Postives = 127/202 (62.87%), Query Frame = 0


Query: 9   FLFIAVVIASSEVRFCRADASPDAVLDTDGKKLRVGDNYYILPAFQGSGGGGLAIGNIRK 68
           FL +AV I+    R    +A+ + V D +GK L  G NYYILP  +G  GGGL + N++ 
Sbjct: 8   FLLLAVFISH---RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGR-GGGLTMSNLKT 67

Query: 69  EYDRCRINVVQEPYEQSDGSPVTFLPINPKKGVVRVSTDLNIEFDASTRCGKSTVWRLGT 128
           E   C  +V+Q+ +E S G PV F P + K   + VSTD+NI+F        +++W L  
Sbjct: 68  E--TCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELAN 127

Query: 129 FNRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVMCKDIGIFE 188
           F+   +Q+F++  G +GNPG++T +NWFKI+K+   YK+ +CPTVC  CKV+C+D+G+F 
Sbjct: 128 FDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFV 187

Query: 189 NTGKRALVLSDTPFPVMFKKVY 211
             GKR L LSD P  VMFK+ Y
Sbjct: 188 QDGKRRLALSDVPLKVMFKRAY 196

[Close]

BLAST of PI0019080 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 131.0 bits (328), Expect = 1.2e-30
Identity = 75/180 (41.67%), Postives = 102/180 (56.67%), Query Frame = 0


Query: 32  AVLDTDGKKLRVGDNYYILPAFQGSGGGGLAIGNIRKEYDRCRINVVQEPYEQSDGSPVT 91
           AV+D DG  +   ++YY+LP  +G GGG    G   +    C  ++VQE  E  +G PV 
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG---RGGQPCPYDIVQESSEVDEGIPVK 88

Query: 92  FLPINPKKGVVRVSTDLNIEFD-ASTRCGKSTVWRLGTFNRYLRQYFVTIGGTKGNPGRE 151
           F     K   V  S +LNIE D  +T C +ST WR+G F+   +QYFV  G      G++
Sbjct: 89  FSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQD 148

Query: 152 TTENWFKIEKYG-NGYKLVYCPTVCEHCKVMCKDIGIF-ENTGKRALVLSDTPFPVMFKK 209
           + +++FKIEK G + YK V+CP  C+     C D+GIF +  G R L LSD PF VMFKK
Sbjct: 149 SLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

[Close]

BLAST of PI0019080 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 114.0 bits (284), Expect = 1.5e-25
Identity = 74/190 (38.95%), Postives = 105/190 (55.26%), Query Frame = 0


Query: 26  ADASP-DAVLDTDGKKLRVGDNYYILPAFQGSGGGGLAIGNIRKEYDRC-RINVVQEPYE 85
           ADA+P   VLD  G  ++    YYI+PA  G+GGG +         D C  +++VQ    
Sbjct: 24  ADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLCLNLDIVQSSSP 83

Query: 86  QSDGSPVTFLPINPKKGVVRVSTDLNIEFDAST-RCGKSTVWRLGTFNRYLRQYFVTIGG 145
              G PVTF P+N K   V++S  LN+EFD++   C  S VWR+   +  LR+ FV+IGG
Sbjct: 84  FVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRI-DHSVQLRKSFVSIGG 143

Query: 146 TKGNPGRETTENWFKIEKYGNGYKLVYCPTVCEHCKVMCKDIGI-FENTGKRALVLS-DT 205
            KG        +WF+I++ G+ YKL+YCP       V C ++ +  ++ G R LVLS D 
Sbjct: 144 QKGK-----GNSWFQIQEDGDAYKLMYCPI---SSIVACINVSLEIDDHGVRRLVLSTDQ 203

Query: 206 PFPVMFKKVY 211
            F V F+K Y
Sbjct: 204 SFVVKFQKAY 204

The following BLAST results are available for this feature:

BLAST of PI0019080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P13087	1.3e-47	49.52	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
Q9LMU2	2.3e-44	43.56	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P32765	3.6e-37	42.86	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	1.6e-29	41.67	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
Q9FX28	2.1e-24	38.95	Kunitz trypsin inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=KTI3 PE=2 SV=1	[more]

BLAST of PI0019080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BJ25	1.1e-105	88.63	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00278...	[more]
A0A1S3AT47	1.1e-105	88.63	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=3 SV=1	[more]
A0A5D3BJ02	3.3e-81	70.33	Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=...	[more]
A0A1S3AST3	3.3e-81	70.33	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1	[more]
A0A0A0LT81	2.1e-80	69.38	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1	[more]

BLAST of PI0019080 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437071.1	2.3e-105	88.63	PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis mel...	[more]
XP_038895396.1	2.3e-81	70.33	miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa his...	[more]
XP_008437082.1	6.7e-81	70.33	PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. mak...	[more]
XP_022923703.1	5.5e-75	66.03	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]
XP_022137472.1	5.5e-75	66.51	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]

BLAST of PI0019080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	1.7e-45	43.56	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	1.2e-30	41.67	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	1.5e-25	38.95	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 73..93 score: 28.38 coord: 155..174 score: 26.47 coord: 32..61 score: 45.1 coord: 178..207 score: 23.66
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 32..210 e-value: 3.3E-70 score: 249.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 33..208 e-value: 1.7E-55 score: 187.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 4..209
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 33..49
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 32..208 e-value: 7.15937E-56 score: 173.297
None	No IPR available	GENE3D	2.80.10.50		coord: 27..209 e-value: 4.2E-71 score: 240.2
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 4..209
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 29..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0019080.1	PI0019080.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity