PI0018742 (gene) Melon (PI 482460) v1

Overview
NamePI0018742
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptiongibberellin 2-beta-dioxygenase 2
Locationchr01: 25513300 .. 25515890 (+)
RNA-Seq ExpressionPI0018742
SyntenyPI0018742
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTGTCCCACATCCATTAGAACCAAAAAAACCAAAGCTGTTGGAATTCCCACCATAGATCTCTCCCAAAGCAAAGCAATAACTTCCAAATCCATAGTTGAAGCTTGCCAAGATTTTGGCTTCTTCAAGCTAATCAACCATGGAGTTTCTCATCATGCCATTGCTCAACTTGAATCCCAAGGCTTTGATTTCTTTCAAAAAACCTCCTCCGAAAAGAACCGAGCCGGTCCTGCCAACCCCTTCGGTTATGGCTGCAAATCCATCGGCTCCAATGGCGACATGGGCGACCTGGAATACCTTCTTCTTCCCTCCCACCACCCAGCTACTTCCATTGTCGAAAGCTCCACCTCCATCTCTAACGACCCTCTTACTTTCAGGTTATAAATATTTTATATTTTAAAAATGTTTCTAATAAGTCGTTGTACTTTTGAAGTTATTTGATCTACCTGTTTTTCTTCATTTAGTTTTTTTTCAAAAAATATTTTAATGGTGGTGACTATAATTTTTATTTTTTAAGTTTTTATATACATTTATTACTTCGTTTTTGGCCAATTTATACGGCTTATTCATTAATCTATTAGATTTATATTTAAACATTTTTAAAGTCTCATATAAAAATGGCCATTAATCTATTTGTAATAGATTACGATCATATTTTGCAATGTTTATAAATATCTTGAGGTCTAAATTTCTATTAGAGAATGCATTTTTATTTTACGAGTCAAACACCTTGAAAGCTGCAAAGATTCATATGTGTGTATATATATTTTCAATATCAAAATGACATTTTTTAATGGAAAAAGGACAATATTTTATCTAATGAGACCAATAGTTTTTATGAAAAAATATATATATATACATTGAGTCCTTATCGATGGAAAAGCATATAATATCATTAGAATATATATATACGATAACTATTTGGTGGTCAATATTTTATATTTTTTTAAAAAGTTTTGCATACGTCTTGATAACTATTTTGTATTTAATAATTGTCCTTCAAAAGTGAATTTTGTTTATATATAGGTTCTTTTTTATATCCTAATTAACTCAAGTTGTAGCTAAAAGAAATCCAACCATTATTTTCTTTTTGTTACCAAATATTAAAGGTTTTTGTATATTCGAAATATATCTTTCTAGAAGCATGACGTTTTAATTTTAGAAAATACAAAAAGAATTATAATTTTAAAATAATTTATGTTTGCAAGGGGTTTTCAAAATAGTTTCATATTGCTTTGTTTTTTAAATTAATTTTATGGAATTATAATTGAGTAGTTTTTTTTAAAAAAAATCAAAATTATTCTCACTTGAAATTATTAATTGAAAGTTTATGCTATTTACCCATAATTAATTTGATTCATATTTGATATATTTTAACTAACCTGCAAGGTAAAAAAAAAGTTTAAATATAAAAATAAAATTATTTTATACTCTATAAAAAGATTATAAACTTCAAGATGCTTGTAAATAACTATTCACAATATTTGAAAATGAGTTACTACTCATAATTCTATTGGCATTTATTTGAATGTTTTTGTCTATATCTCTACGACAGATTGGAAAATTTGATTTCTTGTCGCTTGATATTTTAATTTGTTAATTTGTTGCAGCCATGTTGTGAATGAGTATGTTAAAAGTGTGAGAGACTTAGGTTGCGAGATTCTTGACCTAATTGGTGAAGGTTTATGGTTACCAAACAAATCTGTGTTTAGTAATCTCATTAGAGACGTCCACAGTGATTCAGTTTTGAGGATCAATTACTACCCTGGAGTCCAATACACTGAAAACCGAGATCTTTCAACAAAATCACACCCTTCAAATAACCGAATTGGATTTGGGGAGCATTCTGATCCTCAAATATTGACCATCTTGAGATCCAATGATGTCGATGGCCTTCAAATCTCTTTGCAAGACGGGATTTGGGTACCCGTTTGTCCTGACCCGTCTGCATTCTATGTCTTGGTTGGTGACATTTTTCAGGTAATTAAATATATGTCATATAGTTTCATTTATTTGATTAAATTACGATTGTAATCCATTTTGTTAAATGAATTAGCTAAGTAGCAACGATAAGTTGACATAATACTACTTTTTAAGTTTTTGTATCATATAATTAAAAACACAATGCTTAAAAAAATCTTTAGAATTTATTTTTGTCCTCATATAAAATACTATAGTCGTTGTCACTAAACTCTAACATAAACACATACTTTTGCATAATTAGTTAATGGAAGACAAGCATTTGAAGTCACATGTATTTAGGGTTAGAAATGTCGAGTTTCTAAACATCTTCAAACGACGAATCGAACGTTGAGATTTGTTGTCTTCTGTTTTGAGCACAGGCAATGACAAATGGGAGATTTGTAAGTGTGAGACATAGAGCAATGGCAAACAACTCCAAGAATCCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATACTTGGATTGCACCTCTATCAGAAATGATGCTTCCAAATGACAAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAAAAAGCTGCCTATTCTCTTAGATTAGCAGATTCTCGTCTAGATCTCTTCAAAGCTTCCTAA

mRNA sequence

ATGCCTTGTCCCACATCCATTAGAACCAAAAAAACCAAAGCTGTTGGAATTCCCACCATAGATCTCTCCCAAAGCAAAGCAATAACTTCCAAATCCATAGTTGAAGCTTGCCAAGATTTTGGCTTCTTCAAGCTAATCAACCATGGAGTTTCTCATCATGCCATTGCTCAACTTGAATCCCAAGGCTTTGATTTCTTTCAAAAAACCTCCTCCGAAAAGAACCGAGCCGGTCCTGCCAACCCCTTCGGTTATGGCTGCAAATCCATCGGCTCCAATGGCGACATGGGCGACCTGGAATACCTTCTTCTTCCCTCCCACCACCCAGCTACTTCCATTGTCGAAAGCTCCACCTCCATCTCTAACGACCCTCTTACTTTCAGCCATGTTGTGAATGAGTATGTTAAAAGTGTGAGAGACTTAGGTTGCGAGATTCTTGACCTAATTGGTGAAGGTTTATGGTTACCAAACAAATCTGTGTTTAGTAATCTCATTAGAGACGTCCACAGTGATTCAGTTTTGAGGATCAATTACTACCCTGGAGTCCAATACACTGAAAACCGAGATCTTTCAACAAAATCACACCCTTCAAATAACCGAATTGGATTTGGGGAGCATTCTGATCCTCAAATATTGACCATCTTGAGATCCAATGATGTCGATGGCCTTCAAATCTCTTTGCAAGACGGGATTTGGGTACCCGTTTGTCCTGACCCGTCTGCATTCTATGTCTTGGTTGGTGACATTTTTCAGGCAATGACAAATGGGAGATTTGTAAGTGTGAGACATAGAGCAATGGCAAACAACTCCAAGAATCCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATACTTGGATTGCACCTCTATCAGAAATGATGCTTCCAAATGACAAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAAAAAGCTGCCTATTCTCTTAGATTAGCAGATTCTCGTCTAGATCTCTTCAAAGCTTCCTAA

Coding sequence (CDS)

ATGCCTTGTCCCACATCCATTAGAACCAAAAAAACCAAAGCTGTTGGAATTCCCACCATAGATCTCTCCCAAAGCAAAGCAATAACTTCCAAATCCATAGTTGAAGCTTGCCAAGATTTTGGCTTCTTCAAGCTAATCAACCATGGAGTTTCTCATCATGCCATTGCTCAACTTGAATCCCAAGGCTTTGATTTCTTTCAAAAAACCTCCTCCGAAAAGAACCGAGCCGGTCCTGCCAACCCCTTCGGTTATGGCTGCAAATCCATCGGCTCCAATGGCGACATGGGCGACCTGGAATACCTTCTTCTTCCCTCCCACCACCCAGCTACTTCCATTGTCGAAAGCTCCACCTCCATCTCTAACGACCCTCTTACTTTCAGCCATGTTGTGAATGAGTATGTTAAAAGTGTGAGAGACTTAGGTTGCGAGATTCTTGACCTAATTGGTGAAGGTTTATGGTTACCAAACAAATCTGTGTTTAGTAATCTCATTAGAGACGTCCACAGTGATTCAGTTTTGAGGATCAATTACTACCCTGGAGTCCAATACACTGAAAACCGAGATCTTTCAACAAAATCACACCCTTCAAATAACCGAATTGGATTTGGGGAGCATTCTGATCCTCAAATATTGACCATCTTGAGATCCAATGATGTCGATGGCCTTCAAATCTCTTTGCAAGACGGGATTTGGGTACCCGTTTGTCCTGACCCGTCTGCATTCTATGTCTTGGTTGGTGACATTTTTCAGGCAATGACAAATGGGAGATTTGTAAGTGTGAGACATAGAGCAATGGCAAACAACTCCAAGAATCCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATACTTGGATTGCACCTCTATCAGAAATGATGCTTCCAAATGACAAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAAAAAGCTGCCTATTCTCTTAGATTAGCAGATTCTCGTCTAGATCTCTTCAAAGCTTCCTAA

Protein sequence

MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLESQGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSISNDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPGVQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDKSLYKPFTWAQYKKAAYSLRLADSRLDLFKAS
Homology
BLAST of PI0018742 vs. ExPASy Swiss-Prot
Match: Q9XHM5 (Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.9e-112
Identity = 202/333 (60.66%), Postives = 257/333 (77.18%), Query Frame = 0

Query: 1   MPCPTS-IRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLE 60
           +P PTS IRTKKTKAVGIPTIDLS  ++  S+ +V+AC+++GFFK++NH V    I++L+
Sbjct: 3   VPSPTSMIRTKKTKAVGIPTIDLSLERSQLSELVVKACEEYGFFKVVNHSVPKEVISRLD 62

Query: 61  SQGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSI 120
            +G +FF K SSEK +AG + PFGYGCK+IG NGD G+LEYLLL  H    SI E S +I
Sbjct: 63  EEGIEFFSKNSSEKRQAGTSTPFGYGCKNIGPNGDKGELEYLLL--HSNPISISERSKTI 122

Query: 121 SND-PLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYY 180
           + D P+ FS +VN+Y+K+V+DL CEIL+L  EGLW+P+KS  S +I+D HSDS+LRIN+Y
Sbjct: 123 AKDHPIKFSCIVNDYIKAVKDLTCEILELAAEGLWVPDKSSLSKIIKDEHSDSLLRINHY 182

Query: 181 PGVQY--TENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCP 240
           P V+    +N D S   + +NN IGFGEHSDPQILTILRSN+V GLQIS   G+W+PV P
Sbjct: 183 PPVKKLGNDNWDPSKIQNSNNNNIGFGEHSDPQILTILRSNNVGGLQISTHHGLWIPVPP 242

Query: 241 DPSAFYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMML 300
           DPS FYV+VGD  Q +TNGRFVSVRHR + N +K PRMS+MYF APPLN  I+PLS+M+ 
Sbjct: 243 DPSEFYVMVGDALQVLTNGRFVSVRHRVLTNTTK-PRMSMMYFAAPPLNWLISPLSKMVT 302

Query: 301 PNDKSLYKPFTWAQYKKAAYSLRLADSRLDLFK 330
            +   LY+PFTWAQYK+AAY+LRL D+RLD FK
Sbjct: 303 AHSPCLYRPFTWAQYKQAAYALRLGDTRLDQFK 332

BLAST of PI0018742 vs. ExPASy Swiss-Prot
Match: Q9FZ21 (Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.6e-94
Identity = 176/336 (52.38%), Postives = 225/336 (66.96%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAV-----GIPTIDLS-QSKAITSKSIVEACQDFGFFKLINHGVSHHA 60
           +P  T ++T   K +       P ID S   ++  S+ IV+AC+  GFFK+INHGV    
Sbjct: 3   LPSSTPLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEI 62

Query: 61  IAQLESQGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVE 120
           I + E +G +FF K  S+K RAGPA+PFGYGCK+IG NGD+G+LEYLLL  H   T++ +
Sbjct: 63  IKRFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLL--HANPTAVAD 122

Query: 121 SSTSIS-NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVL 180
            S +IS +DP  FS   N+Y+++VRDL CEI+DL  E LW    S  S LIRDV SDS+L
Sbjct: 123 KSETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSIL 182

Query: 181 RINYYPGVQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVP 240
           R+N+YP   Y  +            +IGFGEHSDPQILT+LRSNDVDGL+I  +DG+W+P
Sbjct: 183 RLNHYPPAPYALS---------GVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIP 242

Query: 241 VCPDPSAFYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSE 300
           +  DP+ F+VLVGD  QA+TNGRF SVRHR +AN +K PRMS MYF APPL   I+PL +
Sbjct: 243 IPSDPTCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPK 302

Query: 301 MMLPNDKSLYKPFTWAQYKKAAYSLRLADSRLDLFK 330
           M+ P +   Y  FTW  YKKA YSLRL   RL+ FK
Sbjct: 303 MVSPENPRRYNSFTWGDYKKATYSLRLDVPRLEFFK 327

BLAST of PI0018742 vs. ExPASy Swiss-Prot
Match: Q9C7Z1 (Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 4.9e-81
Identity = 165/316 (52.22%), Postives = 208/316 (65.82%), Query Frame = 0

Query: 17  IPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLESQGFDFFQKTSSEKNRA 76
           IP ID+SQ ++  S  IV+AC+  GFFK+INHGV    I+++E +  +FF K + EK   
Sbjct: 15  IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74

Query: 77  GPAN-PFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSISNDPLTFSHVVNEYVK 136
            P N PF YG + IG NGD G++EYLL  ++ PA              L+FS  VN Y++
Sbjct: 75  RPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPA----------FRSQLSFSSAVNCYIE 134

Query: 137 SVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYY-PGVQYTENRDLSTKSH 196
           +V+ L  EILDL  EGL +P  S FS LI  V SDSVLR+N+Y P  Q+    +LS +S 
Sbjct: 135 AVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLSDQS- 194

Query: 197 PSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLVGDIFQAMTN 256
            S  R+GFGEH+DPQILT+LRSN V GLQ+S  DG+WV V PDPSAF V VGD+ Q MTN
Sbjct: 195 VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVMTN 254

Query: 257 GRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEM-MLPNDKSLYKPFTWAQYKK 316
           GRF+SVRHRA+    ++ R+S  YF  PPL   I PLS M M  N   LY+ FTW +YKK
Sbjct: 255 GRFISVRHRALTYGEES-RLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWGEYKK 314

Query: 317 AAYSLRLADSRLDLFK 330
            AYSLRL DSRLD+F+
Sbjct: 315 RAYSLRLEDSRLDMFR 317

BLAST of PI0018742 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.6e-78
Identity = 157/314 (50.00%), Postives = 209/314 (66.56%), Query Frame = 0

Query: 12  TKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLESQGFDFFQKTSS 71
           T +  IP +DLS+  A T   IV+AC+DFGFFK+INHG+   AI+QLES+ F FF    +
Sbjct: 22  TFSSSIPLVDLSKPDAKT--LIVKACEDFGFFKVINHGIPLDAISQLESEAFKFFSLPQT 81

Query: 72  EKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSISNDPLTFSHVVN 131
           EK +AGPANPFGYG K IG NGD+G +EYLLL      T+   + +    D   F  ++ 
Sbjct: 82  EKEKAGPANPFGYGNKRIGLNGDIGWIEYLLL-----TTNQDHNFSLYGEDIDKFRGLLK 141

Query: 132 EYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPGVQYTENRDLST 191
           +Y  ++R++ CEILDL+ EGL +  K+VFS L+ D  SD + R+N+YP        +L+ 
Sbjct: 142 DYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFRVNHYPACP-----ELAI 201

Query: 192 KSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLVGDIFQA 251
                 N IGFGEH+DPQI++ILRSN+  G QISL+DG W+ V PD S+F++ VGD  Q 
Sbjct: 202 N---GENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSFFINVGDSLQV 261

Query: 252 MTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDKSLYKPFTWAQY 311
           MTNGRF SVRHR +AN   +PR+S++YF  PPL+  IAPL  +M    +SLYK FTW +Y
Sbjct: 262 MTNGRFKSVRHRVLANGI-DPRLSMIYFCGPPLSEKIAPLPSLM-KGKESLYKEFTWFEY 318

Query: 312 KKAAYSLRLADSRL 326
           K + Y  RLAD+RL
Sbjct: 322 KSSTYGSRLADNRL 318

BLAST of PI0018742 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.6e-78
Identity = 150/316 (47.47%), Postives = 209/316 (66.14%), Query Frame = 0

Query: 16  GIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLESQGFDFFQKTSSEKNR 75
           GIP +DL+   A     IV AC+DFGFFKL+NHGV    +A LE++   FF+K+ SEK+R
Sbjct: 26  GIPVVDLTHPDA--KNLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQSEKDR 85

Query: 76  AGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSISNDPLTFSHVVNEYVK 135
           AGP +PFGYG K IG NGD+G +EYLLL + +P     +S      +P  F  VV  Y+ 
Sbjct: 86  AGPPDPFGYGSKRIGPNGDVGWVEYLLL-NTNPDVISPKSLCIFRENPHHFRAVVENYIT 145

Query: 136 SVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPGVQYTENRDLSTKSHP 195
           +V+++   +L+L+ EGL +  ++  S L++D  SDS  R+N+YP     +          
Sbjct: 146 AVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPCPEVQAL-------- 205

Query: 196 SNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLVGDIFQAMTNG 255
           + N +GFGEH+DPQI+++LRSN   GLQI L DG WV V PD ++F++ VGD  Q MTNG
Sbjct: 206 NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTNG 265

Query: 256 RFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDKSLYKPFTWAQYKKAA 315
           RF SV+HR +A+ +K+ R+S++YFG P L+  IAPL  +ML  ++ LYK FTW +YKKAA
Sbjct: 266 RFKSVKHRVLADTTKS-RLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKAA 325

Query: 316 YSLRLADSRLDLFKAS 332
           Y+ RLAD+RL  F+ S
Sbjct: 326 YTSRLADNRLAPFQKS 329

BLAST of PI0018742 vs. ExPASy TrEMBL
Match: S0BDY8 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox2 PE=2 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 3.8e-185
Identity = 317/330 (96.06%), Postives = 327/330 (99.09%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPTSIRTKKT+AVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES
Sbjct: 1   MPCPTSIRTKKTRAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
           QGFDFFQK SSEK+RAGPANPFGYGCKSIGSNGDMGDLEYLLLPSH PATSI++SSTSIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHDPATSILQSSTSIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           NDPLTFSHVVNEYVK VRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKGVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           VQ+TENR+LSTKSHPSNNRIGFGEHSDPQ+LTILR+NDVDGLQISLQDGIWVPVCPDPSA
Sbjct: 181 VQHTENRELSTKSHPSNNRIGFGEHSDPQVLTILRANDVDGLQISLQDGIWVPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD+FQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKA 331
           SLYKPFTWAQYKKAAYSLRLADSRLDLFKA
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKA 330

BLAST of PI0018742 vs. ExPASy TrEMBL
Match: A0A0A0K6E5 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G413380 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.4e-184
Identity = 315/330 (95.45%), Postives = 327/330 (99.09%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPTSIRTKKT+AVGIPTIDLSQSKAITSKSI+EACQDFGFFKLINHGVSHHAIAQLES
Sbjct: 1   MPCPTSIRTKKTRAVGIPTIDLSQSKAITSKSIIEACQDFGFFKLINHGVSHHAIAQLES 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
           QGFDFFQK SSEK+RAGPANPFGYGCKSIGSNGDMGDLEYLLLPSH PATSI++SSTSIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHDPATSILQSSTSIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           NDPLTFSHVVNEYVK VRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKGVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           VQ+TENR+LSTKSHPSNNRIGFGEHSDPQ+LTILR+NDVDGLQISL+DGIWVPVCPDPSA
Sbjct: 181 VQHTENRELSTKSHPSNNRIGFGEHSDPQVLTILRANDVDGLQISLRDGIWVPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD+FQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKA 331
           SLYKPFTWAQYKKAAYSLRLADSRLDLFKA
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKA 330

BLAST of PI0018742 vs. ExPASy TrEMBL
Match: A0A1S3CFZ0 (gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo OX=3656 GN=LOC103500006 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.4e-179
Identity = 311/331 (93.96%), Postives = 323/331 (97.58%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPTSIRTKKTKAVGIPTIDLSQS+AITSKSIVEACQ+FGFFKLINHGV HHAIAQLES
Sbjct: 1   MPCPTSIRTKKTKAVGIPTIDLSQSRAITSKSIVEACQEFGFFKLINHGVPHHAIAQLES 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
           QGFDFFQK SSEK+RAGP NPFGYGCKSIGSNGDMGDLEYLLLPSH+PATSI++S  SIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPPNPFGYGCKSIGSNGDMGDLEYLLLPSHNPATSILQS--SIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           VQ+ ENR+LSTKSHPSN+RIGFGEHSDPQILTILR+NDVDGLQISLQD IWVPVCPDPSA
Sbjct: 181 VQHIENRELSTKSHPSNDRIGFGEHSDPQILTILRANDVDGLQISLQDSIWVPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD+FQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAP LNTWIAPLSEMMLPNDK
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPSLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329

BLAST of PI0018742 vs. ExPASy TrEMBL
Match: A0A5D3BE10 (Gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00010 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.1e-179
Identity = 310/331 (93.66%), Postives = 323/331 (97.58%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPTSIRTKKTKAVGIPTIDLSQS+AITSKSIVEACQ+FGFFKLINHGV HHAIAQLES
Sbjct: 1   MPCPTSIRTKKTKAVGIPTIDLSQSRAITSKSIVEACQEFGFFKLINHGVPHHAIAQLES 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
           QGFDFFQK SSEK+RAGP NPFGYGCKSIGSNGDMGDLEYLLLPSH+PATSI++S  SIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPPNPFGYGCKSIGSNGDMGDLEYLLLPSHNPATSILQS--SIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRD+HSDSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDIHSDSVLRINYYPG 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           VQ+ ENR+LSTKSHPSN+RIGFGEHSDPQILTILR+NDVDGLQISLQD IWVPVCPDPSA
Sbjct: 181 VQHIENRELSTKSHPSNDRIGFGEHSDPQILTILRANDVDGLQISLQDSIWVPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD+FQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAP LNTWIAPLSEMMLPNDK
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPSLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329

BLAST of PI0018742 vs. ExPASy TrEMBL
Match: A0A6J1GRC3 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456420 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 2.8e-156
Identity = 275/331 (83.08%), Postives = 302/331 (91.24%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPT++RTKKTKAVGIPTIDLSQ++  TSKSI+ AC  FGFFKLINHGVSHHAI++LE 
Sbjct: 1   MPCPTAMRTKKTKAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
             FDFF KT+ EK+RAGPA PFGYG K+IG NGD GDLEYLLL S  PA S++++S SIS
Sbjct: 61  HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRS-DPA-SVLQTSPSIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           NDP TFS+VVNEYVKSV+DLGCEILDL+GEGLWLPNKSVFS+LIRDVHSDSVLRINYYP 
Sbjct: 121 NDPSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPA 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           V++TEN +LS+KS+PSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDG+W PVCPDPSA
Sbjct: 181 VKHTENWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD  QAMTNGRFVSVRHRAMANNSK  RMSIMYFGAPPLN WIAPLSEM+LPN +
Sbjct: 241 FYVLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEMILPNHQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329

BLAST of PI0018742 vs. NCBI nr
Match: NP_001292648.1 (gibberellin 2-beta-dioxygenase 2 [Cucumis sativus] >CCD28486.1 GA 2-oxidase [Cucumis sativus])

HSP 1 Score: 657.1 bits (1694), Expect = 7.8e-185
Identity = 317/330 (96.06%), Postives = 327/330 (99.09%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPTSIRTKKT+AVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES
Sbjct: 1   MPCPTSIRTKKTRAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
           QGFDFFQK SSEK+RAGPANPFGYGCKSIGSNGDMGDLEYLLLPSH PATSI++SSTSIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHDPATSILQSSTSIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           NDPLTFSHVVNEYVK VRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKGVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           VQ+TENR+LSTKSHPSNNRIGFGEHSDPQ+LTILR+NDVDGLQISLQDGIWVPVCPDPSA
Sbjct: 181 VQHTENRELSTKSHPSNNRIGFGEHSDPQVLTILRANDVDGLQISLQDGIWVPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD+FQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKA 331
           SLYKPFTWAQYKKAAYSLRLADSRLDLFKA
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKA 330

BLAST of PI0018742 vs. NCBI nr
Match: KGN45038.1 (hypothetical protein Csa_016303 [Cucumis sativus])

HSP 1 Score: 655.2 bits (1689), Expect = 3.0e-184
Identity = 315/330 (95.45%), Postives = 327/330 (99.09%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPTSIRTKKT+AVGIPTIDLSQSKAITSKSI+EACQDFGFFKLINHGVSHHAIAQLES
Sbjct: 1   MPCPTSIRTKKTRAVGIPTIDLSQSKAITSKSIIEACQDFGFFKLINHGVSHHAIAQLES 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
           QGFDFFQK SSEK+RAGPANPFGYGCKSIGSNGDMGDLEYLLLPSH PATSI++SSTSIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHDPATSILQSSTSIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           NDPLTFSHVVNEYVK VRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKGVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           VQ+TENR+LSTKSHPSNNRIGFGEHSDPQ+LTILR+NDVDGLQISL+DGIWVPVCPDPSA
Sbjct: 181 VQHTENRELSTKSHPSNNRIGFGEHSDPQVLTILRANDVDGLQISLRDGIWVPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD+FQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKA 331
           SLYKPFTWAQYKKAAYSLRLADSRLDLFKA
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKA 330

BLAST of PI0018742 vs. NCBI nr
Match: XP_008461412.1 (PREDICTED: gibberellin 2-beta-dioxygenase 2 [Cucumis melo])

HSP 1 Score: 637.9 bits (1644), Expect = 4.9e-179
Identity = 311/331 (93.96%), Postives = 323/331 (97.58%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPTSIRTKKTKAVGIPTIDLSQS+AITSKSIVEACQ+FGFFKLINHGV HHAIAQLES
Sbjct: 1   MPCPTSIRTKKTKAVGIPTIDLSQSRAITSKSIVEACQEFGFFKLINHGVPHHAIAQLES 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
           QGFDFFQK SSEK+RAGP NPFGYGCKSIGSNGDMGDLEYLLLPSH+PATSI++S  SIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPPNPFGYGCKSIGSNGDMGDLEYLLLPSHNPATSILQS--SIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           VQ+ ENR+LSTKSHPSN+RIGFGEHSDPQILTILR+NDVDGLQISLQD IWVPVCPDPSA
Sbjct: 181 VQHIENRELSTKSHPSNDRIGFGEHSDPQILTILRANDVDGLQISLQDSIWVPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD+FQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAP LNTWIAPLSEMMLPNDK
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPSLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329

BLAST of PI0018742 vs. NCBI nr
Match: KAA0058535.1 (gibberellin 2-beta-dioxygenase 2 [Cucumis melo var. makuwa] >TYJ96891.1 gibberellin 2-beta-dioxygenase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 637.5 bits (1643), Expect = 6.4e-179
Identity = 310/331 (93.66%), Postives = 323/331 (97.58%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPTSIRTKKTKAVGIPTIDLSQS+AITSKSIVEACQ+FGFFKLINHGV HHAIAQLES
Sbjct: 1   MPCPTSIRTKKTKAVGIPTIDLSQSRAITSKSIVEACQEFGFFKLINHGVPHHAIAQLES 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
           QGFDFFQK SSEK+RAGP NPFGYGCKSIGSNGDMGDLEYLLLPSH+PATSI++S  SIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPPNPFGYGCKSIGSNGDMGDLEYLLLPSHNPATSILQS--SIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRD+HSDSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDIHSDSVLRINYYPG 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           VQ+ ENR+LSTKSHPSN+RIGFGEHSDPQILTILR+NDVDGLQISLQD IWVPVCPDPSA
Sbjct: 181 VQHIENRELSTKSHPSNDRIGFGEHSDPQILTILRANDVDGLQISLQDSIWVPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD+FQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAP LNTWIAPLSEMMLPNDK
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPSLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329

BLAST of PI0018742 vs. NCBI nr
Match: XP_038896324.1 (gibberellin 2-beta-dioxygenase 2 [Benincasa hispida])

HSP 1 Score: 587.8 bits (1514), Expect = 5.8e-164
Identity = 291/331 (87.92%), Postives = 308/331 (93.05%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60
           MPCPT++RTKKTKAVGIPTIDLSQS+AITSKSIVEACQDFGFFKLINHGVS  AIAQLES
Sbjct: 1   MPCPTAMRTKKTKAVGIPTIDLSQSRAITSKSIVEACQDFGFFKLINHGVSDDAIAQLES 60

Query: 61  QGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSIS 120
           QGFDFF+KT+ EK+ AGPANPFGYGCKSIG NGDMGDLEYLLL S    +SI++ S SIS
Sbjct: 61  QGFDFFEKTALEKHCAGPANPFGYGCKSIGPNGDMGDLEYLLLRS--DPSSILQCSPSIS 120

Query: 121 NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180
           N PLTFSHVVNEYVKSVRDLGCEILDL+ EGLWLPNKSVFSNLIRDVH+DSVLRINYYPG
Sbjct: 121 NHPLTFSHVVNEYVKSVRDLGCEILDLVTEGLWLPNKSVFSNLIRDVHNDSVLRINYYPG 180

Query: 181 VQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           V++TEN DLS KS  SNNRIGFGEHSDPQILTILRSNDV+GLQISLQDGIW PVCPDPSA
Sbjct: 181 VKHTENWDLSPKSCLSNNRIGFGEHSDPQILTILRSNDVNGLQISLQDGIWAPVCPDPSA 240

Query: 241 FYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300
           FYVL+GD FQAMTNGRFVSVRHRAMANNSKN RMSIMYFGAPPLN WIAPLSEMMLPN++
Sbjct: 241 FYVLIGDAFQAMTNGRFVSVRHRAMANNSKNARMSIMYFGAPPLNAWIAPLSEMMLPNEQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLDLFK S
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKTS 329

BLAST of PI0018742 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 345.1 bits (884), Expect = 6.1e-95
Identity = 176/336 (52.38%), Postives = 225/336 (66.96%), Query Frame = 0

Query: 1   MPCPTSIRTKKTKAV-----GIPTIDLS-QSKAITSKSIVEACQDFGFFKLINHGVSHHA 60
           +P  T ++T   K +       P ID S   ++  S+ IV+AC+  GFFK+INHGV    
Sbjct: 3   LPSSTPLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEI 62

Query: 61  IAQLESQGFDFFQKTSSEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVE 120
           I + E +G +FF K  S+K RAGPA+PFGYGCK+IG NGD+G+LEYLLL  H   T++ +
Sbjct: 63  IKRFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLL--HANPTAVAD 122

Query: 121 SSTSIS-NDPLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVL 180
            S +IS +DP  FS   N+Y+++VRDL CEI+DL  E LW    S  S LIRDV SDS+L
Sbjct: 123 KSETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSIL 182

Query: 181 RINYYPGVQYTENRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVP 240
           R+N+YP   Y  +            +IGFGEHSDPQILT+LRSNDVDGL+I  +DG+W+P
Sbjct: 183 RLNHYPPAPYALS---------GVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIP 242

Query: 241 VCPDPSAFYVLVGDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSE 300
           +  DP+ F+VLVGD  QA+TNGRF SVRHR +AN +K PRMS MYF APPL   I+PL +
Sbjct: 243 IPSDPTCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPK 302

Query: 301 MMLPNDKSLYKPFTWAQYKKAAYSLRLADSRLDLFK 330
           M+ P +   Y  FTW  YKKA YSLRL   RL+ FK
Sbjct: 303 MVSPENPRRYNSFTWGDYKKATYSLRLDVPRLEFFK 327

BLAST of PI0018742 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 302.8 bits (774), Expect = 3.5e-82
Identity = 165/316 (52.22%), Postives = 208/316 (65.82%), Query Frame = 0

Query: 17  IPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLESQGFDFFQKTSSEKNRA 76
           IP ID+SQ ++  S  IV+AC+  GFFK+INHGV    I+++E +  +FF K + EK   
Sbjct: 15  IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74

Query: 77  GPAN-PFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSISNDPLTFSHVVNEYVK 136
            P N PF YG + IG NGD G++EYLL  ++ PA              L+FS  VN Y++
Sbjct: 75  RPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPA----------FRSQLSFSSAVNCYIE 134

Query: 137 SVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYY-PGVQYTENRDLSTKSH 196
           +V+ L  EILDL  EGL +P  S FS LI  V SDSVLR+N+Y P  Q+    +LS +S 
Sbjct: 135 AVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLSDQS- 194

Query: 197 PSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLVGDIFQAMTN 256
            S  R+GFGEH+DPQILT+LRSN V GLQ+S  DG+WV V PDPSAF V VGD+ Q MTN
Sbjct: 195 VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVMTN 254

Query: 257 GRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEM-MLPNDKSLYKPFTWAQYKK 316
           GRF+SVRHRA+    ++ R+S  YF  PPL   I PLS M M  N   LY+ FTW +YKK
Sbjct: 255 GRFISVRHRALTYGEES-RLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWGEYKK 314

Query: 317 AAYSLRLADSRLDLFK 330
            AYSLRL DSRLD+F+
Sbjct: 315 RAYSLRLEDSRLDMFR 317

BLAST of PI0018742 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 282.7 bits (722), Expect = 3.7e-76
Identity = 150/324 (46.30%), Postives = 205/324 (63.27%), Query Frame = 0

Query: 11  KTKAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLESQGFDFFQKTS 70
           K + V IP IDL+ S A T   IV+AC++FGFFK+INHGV    + QLE +  +FF    
Sbjct: 21  KPRPVLIPVIDLTDSDAKT--QIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHH 80

Query: 71  SEKNRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSH-----HPATSIVESSTSISNDPLT 130
           S K++AGP +PFGYG K IG NGD+G LEY+LL ++     H  T+I        + P  
Sbjct: 81  SLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAI------FRHTPAI 140

Query: 131 FSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPGVQYTE 190
           F   V EY+K ++ +  + L+++ E L +  K   S L++   SDS LR+N+YP  + T 
Sbjct: 141 FREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEET- 200

Query: 191 NRDLSTKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLV 250
                    P    IGFGEH+DPQ++++LRSND +GLQI ++DG WV V PD S+F+VLV
Sbjct: 201 ---------PVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLV 260

Query: 251 GDIFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDKSLYKP 310
           GD  Q MTNGRF SV+HR +  N+K  R+S++YF  PPL+  IAPLS ++   D  LY  
Sbjct: 261 GDTLQVMTNGRFKSVKHR-VVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNE 320

Query: 311 FTWAQYKKAAYSLRLADSRLDLFK 330
           FTW+QYK +AY  +L D RL LF+
Sbjct: 321 FTWSQYKLSAYKTKLGDYRLGLFE 325

BLAST of PI0018742 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 277.3 bits (708), Expect = 1.6e-74
Identity = 139/313 (44.41%), Postives = 204/313 (65.18%), Query Frame = 0

Query: 17  IPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLESQGFDFFQKTSSEKNRA 76
           IP ++L+  +A T   IV+AC++FGFFK++NHGV    + +LE +   FF    S KNRA
Sbjct: 31  IPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRA 90

Query: 77  GPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSISNDPLTFSHVVNEYVKS 136
           GP  P+GYG K IG NGD+G +EYLLL + +P  S  ++S      P  F   V EY+K 
Sbjct: 91  GPPEPYGYGNKRIGPNGDVGWIEYLLL-NANPQLSSPKTSAVFRQTPQIFRESVEEYMKE 150

Query: 137 VRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPGVQYTENRDLSTKSHPS 196
           ++++  ++L+++ E L +  +   S ++RD  SDS LR+N+YP  +    + +       
Sbjct: 151 IKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEAEKMV------- 210

Query: 197 NNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLVGDIFQAMTNGR 256
             ++GFGEH+DPQI+++LRSN+  GLQI ++DG WV V PD S+F++ VGD  Q MTNGR
Sbjct: 211 --KVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGR 270

Query: 257 FVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDKSLYKPFTWAQYKKAAY 316
           F SV+HR +A +++  R+S++YFG PPL+  IAPL  ++   D  LYK FTW+QYK +AY
Sbjct: 271 FKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAY 330

Query: 317 SLRLADSRLDLFK 330
             +L D RL LF+
Sbjct: 331 KSKLGDYRLGLFE 330

BLAST of PI0018742 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 268.1 bits (684), Expect = 9.5e-72
Identity = 139/313 (44.41%), Postives = 204/313 (65.18%), Query Frame = 0

Query: 17  IPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLESQGFDFFQKTSSEKNRA 76
           IP ID+S  +  +  ++V+AC+DFGFFK+INHGVS   ++ LE +  DFF    SEK + 
Sbjct: 18  IPVIDMSDPE--SKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV 77

Query: 77  GPANPFGYGCKSIGSNGDMGDLEYLLLPSHHPATSIVESSTSISNDPLTFSHVVNEYVKS 136
               PFGYG   IG NGD+G +EYLL+ ++H + S      S+   P TF + + EY  S
Sbjct: 78  A-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGS-GPLFPSLLKSPGTFRNALEEYTTS 137

Query: 137 VRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPGVQYTENRDLSTKSHPS 196
           VR +  ++L+ I +GL +  ++  S L+ D ++DS+LR+N+YP    +     + K++  
Sbjct: 138 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLS-----NKKTNGG 197

Query: 197 NNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLVGDIFQAMTNGR 256
            N IGFGEH+DPQI+++LRSN+  GLQI+L DG W+ V PD ++F+  VGD  Q MTNGR
Sbjct: 198 KNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGR 257

Query: 257 FVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDKSLYKPFTWAQYKKAAY 316
           F SVRHR +A N K  R+S++YF  P L   IAPL+ ++   D+ LY+ FTW++YK + Y
Sbjct: 258 FKSVRHRVLA-NCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTY 317

Query: 317 SLRLADSRLDLFK 330
           + RL+D+RL  F+
Sbjct: 318 NSRLSDNRLQQFE 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XHM51.9e-11260.66Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1[more]
Q9FZ218.6e-9452.38Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 ... [more]
Q9C7Z14.9e-8152.22Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 ... [more]
Q9SQ806.6e-7850.00Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1[more]
Q9XG836.6e-7847.47Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
S0BDY83.8e-18596.06GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox2 PE=2 SV=1[more]
A0A0A0K6E51.4e-18495.45Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... [more]
A0A1S3CFZ02.4e-17993.96gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo OX=3656 GN=LOC103500006 PE=3 SV... [more]
A0A5D3BE103.1e-17993.66Gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1GRC32.8e-15683.08gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
Match NameE-valueIdentityDescription
NP_001292648.17.8e-18596.06gibberellin 2-beta-dioxygenase 2 [Cucumis sativus] >CCD28486.1 GA 2-oxidase [Cuc... [more]
KGN45038.13.0e-18495.45hypothetical protein Csa_016303 [Cucumis sativus][more]
XP_008461412.14.9e-17993.96PREDICTED: gibberellin 2-beta-dioxygenase 2 [Cucumis melo][more]
KAA0058535.16.4e-17993.66gibberellin 2-beta-dioxygenase 2 [Cucumis melo var. makuwa] >TYJ96891.1 gibberel... [more]
XP_038896324.15.8e-16487.92gibberellin 2-beta-dioxygenase 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G02400.16.1e-9552.38gibberellin 2-oxidase 6 [more]
AT1G47990.13.5e-8252.22gibberellin 2-oxidase 4 [more]
AT2G34555.13.7e-7646.30gibberellin 2-oxidase 3 [more]
AT1G30040.11.6e-7444.41gibberellin 2-oxidase [more]
AT1G78440.19.5e-7244.41Arabidopsis thaliana gibberellin 2-oxidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 33..50
score: 53.03
coord: 241..267
score: 25.59
coord: 205..226
score: 32.23
NoneNo IPR availablePANTHERPTHR47990:SF45GIBBERELLIN 2-BETA-DIOXYGENASE 6coord: 2..329
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 2..329
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 9..321
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 3..319
e-value: 5.3E-71
score: 241.6
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 170..280
e-value: 1.4E-21
score: 76.8
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 17..89
e-value: 6.9E-13
score: 49.4
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 168..290
score: 11.426297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0018742.1PI0018742.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045487 gibberellin catabolic process
biological_process GO:0009416 response to light stimulus
molecular_function GO:0052634 C-19 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding