PI0018446 (gene) Melon (PI 482460) v1

Overview
NamePI0018446
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionMetal tolerance protein B
Locationchr12: 23869284 .. 23870395 (+)
RNA-Seq ExpressionPI0018446
SyntenyPI0018446
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAATAGTTGCTAAGAAGAGGAATGTTGTTATTCCCATTTCCTCAGAAGTTAAATGTTGCAATTCTGGCTGTGCTTTTTCCAGATTAGAACATAGTAATTTGGAGTCATTAAAACGTTCTAAATCAGCTATGAAGCTTGGTGGATTGATCTTGTTTTATACCATAGCTATAGTAGTGGAGATTATTGGTGGTTTGAGAGCTAATAGTCTTTCTGTAATGACAGATGCAGCTCACTTGCTTAGTGATGTTGCTGGTTTCTCTGTTTCCCTCTTTGCCGTTTGGGTTTCGGGTTGGGAGGCGACACCGCAACACTCATTCGGGTATAACCGTCTTGAAGTTTTAGGTGCCCTTGTGTCTGTACAGCTTATATGGCTGATTTCTGGGGTATTAATCTACGAGGCGATCGACCGGATTCTTGCACAAAAGACTAAGGTGGATGGTTTCCTTATGTTTGCCATTGCAGCTTTTGGATTCATTCTTAACTTGTTTATGGTTATTTGGCTTGGCCATAGCCACCACCACCACCACTCTCATTCATCTCATTGTTGTCATCATGATCATCACTCTCGTTCTCATCAGAATCACTTGGATCATGAGTCTGACCATGAGGAGGAGGAGATTTACACATTAACCAAACAAGAAGGTGCTTCTTGGGATCAAAGGACAATAGTTCAACGTTGAACATAAATCTCCAAGGTGCTTATCTTCATGTGATTACTGATATGATTCAATCTATTGGTGTGATGATTGCTGGATTCGTTCTTTGGTTTAAACCCGAGTGGATCATCGTCGATTTAATCTGCACTCTTGTATTTTCTGTTCTTGCTTTAGCCACTACTTTCTCTATGCTTAGACATATAGCTGTTATATTGATGGAAGGAACACCAAGGGAGGTCCATATTGAGAGCTTAGAAAATGAAATCAAGAACATAAAAGGAGTCCATGATTTGCATGACTTGCACATTTGGTCCATAACGGTTGGAAAAGTCGTGTTGTCGTGCCATGTTGTTGCTGAAGCCGGAGTTTGTTCTAGGGAGCTAATCTTGAAGATTAAAAGCCATTGTGAAAATAGATACAATATAGTCCATACCACCATACAAGTTGAGTAG

mRNA sequence

ATGCCAATAGTTGCTAAGAAGAGGAATGTTGTTATTCCCATTTCCTCAGAAGTTAAATGTTGCAATTCTGGCTGTGCTTTTTCCAGATTAGAACATAGTAATTTGGAGTCATTAAAACGTTCTAAATCAGCTATGAAGCTTGGTGGATTGATCTTGTTTTATACCATAGCTATAGTAGTGGAGATTATTGGTGGTTTGAGAGCTAATAGTCTTTCTGTAATGACAGATGCAGCTCACTTGCTTAGTGATGTTGCTGGTTTCTCTGTTTCCCTCTTTGCCGTTTGGGTTTCGGGTTGGGAGGCGACACCGCAACACTCATTCGGGTATAACCGTCTTGAAGTTTTAGGTGCCCTTGTGTCTGTACAGCTTATATGGCTGATTTCTGGGGTATTAATCTACGAGGCGATCGACCGGATTCTTGCACAAAAGACTAAGGTGGATGGTTTCCTTATGTTTGCCATTGCAGCTTTTGGATTCATTCTTAACTTGTTTATGGTTATTTGGCTTGGCCATAGCCACCACCACCACCACTCTCATTCATCTCATTGTTGTCATCATGATCATCACTCTCGTTCTCATCAGAATCACTTGGATCATGAAAGGTGCTTCTTGGGATCAAAGGACAATAGTTCAACGTTGAACATAAATCTCCAAGGTGCTTATCTTCATGTGATTACTGATATGATTCAATCTATTGGTGTGATGATTGCTGGATTCGTTCTTTGGTTTAAACCCGAGTGGATCATCGTCGATTTAATCTGCACTCTTGTATTTTCTGTTCTTGCTTTAGCCACTACTTTCTCTATGCTTAGACATATAGCTGTTATATTGATGGAAGGAACACCAAGGGAGGTCCATATTGAGAGCTTAGAAAATGAAATCAAGAACATAAAAGGAGTCCATGATTTGCATGACTTGCACATTTGGTCCATAACGGTTGGAAAAGTCGTGTTGTCGTGCCATGTTGTTGCTGAAGCCGGAGTTTGTTCTAGGGAGCTAATCTTGAAGATTAAAAGCCATTGTGAAAATAGATACAATATAGTCCATACCACCATACAAGTTGAGTAG

Coding sequence (CDS)

ATGCCAATAGTTGCTAAGAAGAGGAATGTTGTTATTCCCATTTCCTCAGAAGTTAAATGTTGCAATTCTGGCTGTGCTTTTTCCAGATTAGAACATAGTAATTTGGAGTCATTAAAACGTTCTAAATCAGCTATGAAGCTTGGTGGATTGATCTTGTTTTATACCATAGCTATAGTAGTGGAGATTATTGGTGGTTTGAGAGCTAATAGTCTTTCTGTAATGACAGATGCAGCTCACTTGCTTAGTGATGTTGCTGGTTTCTCTGTTTCCCTCTTTGCCGTTTGGGTTTCGGGTTGGGAGGCGACACCGCAACACTCATTCGGGTATAACCGTCTTGAAGTTTTAGGTGCCCTTGTGTCTGTACAGCTTATATGGCTGATTTCTGGGGTATTAATCTACGAGGCGATCGACCGGATTCTTGCACAAAAGACTAAGGTGGATGGTTTCCTTATGTTTGCCATTGCAGCTTTTGGATTCATTCTTAACTTGTTTATGGTTATTTGGCTTGGCCATAGCCACCACCACCACCACTCTCATTCATCTCATTGTTGTCATCATGATCATCACTCTCGTTCTCATCAGAATCACTTGGATCATGAAAGGTGCTTCTTGGGATCAAAGGACAATAGTTCAACGTTGAACATAAATCTCCAAGGTGCTTATCTTCATGTGATTACTGATATGATTCAATCTATTGGTGTGATGATTGCTGGATTCGTTCTTTGGTTTAAACCCGAGTGGATCATCGTCGATTTAATCTGCACTCTTGTATTTTCTGTTCTTGCTTTAGCCACTACTTTCTCTATGCTTAGACATATAGCTGTTATATTGATGGAAGGAACACCAAGGGAGGTCCATATTGAGAGCTTAGAAAATGAAATCAAGAACATAAAAGGAGTCCATGATTTGCATGACTTGCACATTTGGTCCATAACGGTTGGAAAAGTCGTGTTGTCGTGCCATGTTGTTGCTGAAGCCGGAGTTTGTTCTAGGGAGCTAATCTTGAAGATTAAAAGCCATTGTGAAAATAGATACAATATAGTCCATACCACCATACAAGTTGAGTAG

Protein sequence

MPIVAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHSSHCCHHDHHSRSHQNHLDHERCFLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNIVHTTIQVE
Homology
BLAST of PI0018446 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 5.8e-96
Identity = 182/343 (53.06%), Postives = 242/343 (70.55%), Query Frame = 0

Query: 25  CAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDV 84
           CAF+R EH   E+ +R +S  +L  LI  Y I + V+I+GG +ANSL+VMTDAAHLLSDV
Sbjct: 36  CAFTRQEHCVSETKEREESTRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDV 95

Query: 85  AGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKT 144
           AG  VSL A+ VS WEA P++SFG+ RLEVL A +SVQLIWL+SGV+I+EAI R+L++  
Sbjct: 96  AGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSR 155

Query: 145 KVDGFLMFAIAAFGFILNLFMVIWLGHSH-HHHHSHSSHCCHHDHHSRSHQNHLDHERC- 204
           +V+G +MF I+AFGF +NL MV+WLGH+H HHHH H     HH HH+  HQ+   H+   
Sbjct: 156 EVNGEIMFGISAFGFFMNLVMVLWLGHNHSHHHHDH-----HHHHHNHKHQHQHHHKEVV 215

Query: 205 ----------FLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIVDL 264
                       G K +S  +NIN+QGAYLH + DMIQS+GVMI G ++W KP+W++VDL
Sbjct: 216 AEEEEEEMNPLKGEKSSSKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDL 275

Query: 265 ICTLVFSVLALATTFSMLRHIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWSIT 324
           ICTLVFS  ALA T  +L++I  ILME  PR++ IE LE  +K I GV  ++DLH+W IT
Sbjct: 276 ICTLVFSAFALAATLPILKNIFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEIT 335

Query: 325 VGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNIVHTTIQVE 356
           VG++VLSCH++ E G   +E+I  +++ C   Y I H T+QVE
Sbjct: 336 VGRIVLSCHILPEPGASPKEIITGVRNFCRKSYGIYHATVQVE 373

BLAST of PI0018446 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 312.0 bits (798), Expect = 8.7e-84
Identity = 174/384 (45.31%), Postives = 249/384 (64.84%), Query Frame = 0

Query: 4   VAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEII 63
           V K     I ++S+V C  + C FS  ++++ ++ +RS S  KL   ++   + + VE++
Sbjct: 16  VGKSDEERIIVASKV-CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVV 75

Query: 64  GGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQL 123
           GG++ANSL+++TDAAHLLSDVA F++SLF++W +GWEATP+ ++G+ R+E+LGALVS+QL
Sbjct: 76  GGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQL 135

Query: 124 IWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHSSHC 183
           IWL++G+L+YEAI RI+ + ++V+GFLMF +AAFG ++N+ M + LGH H H H H  H 
Sbjct: 136 IWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGH-GHG 195

Query: 184 CHHDHHSRS-------HQNHLDHERCFLGSK----------------DNSST-------- 243
             HDHH+ S       H +H DHE                       D S T        
Sbjct: 196 HGHDHHNHSHGVTVTTHHHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKR 255

Query: 244 -LNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLR 303
             NINLQGAYLHV+ D IQS+GVMI G ++W+ PEW IVDLICTL FSV+ L TT +M+R
Sbjct: 256 KRNINLQGAYLHVLGDSIQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIR 315

Query: 304 HIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSR 356
           +I  +LME TPRE+    LE  +  ++ V  +H+LHIW+ITVGKV+L+CHV       + 
Sbjct: 316 NILEVLMESTPREIDATKLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADAD 375

BLAST of PI0018446 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 9.6e-83
Identity = 173/386 (44.82%), Postives = 243/386 (62.95%), Query Frame = 0

Query: 20  CCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 79
           C  + C FS   +S+ ++ +R  S  KL   ++   I + VE++GG++ANSL+++TDAAH
Sbjct: 31  CRGAACDFSDSSNSSKDARERMASMRKLIIAVILCIIFMAVEVVGGIKANSLAILTDAAH 90

Query: 80  LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRI 139
           LLSDVA F++SLF++W +GWEATPQ S+G+ R+E+LGALVS+QLIWL++G+L+YEAI R+
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPQQSYGFFRIEILGALVSIQLIWLLAGILVYEAIVRL 150

Query: 140 LAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSH--------------SSHCCH 199
           + +  +V G LMFA++AFG  +N+ M + LGH H H H H              S H  H
Sbjct: 151 INESGEVQGSLMFAVSAFGLFVNIIMAVLLGHDHGHGHGHGHGHGHSHDHDHGGSDHDHH 210

Query: 200 H---------DHHSRSHQN---------------HLDHERCFL-----------GSKD-N 259
           H          HH   H N               H D E   L           G+KD  
Sbjct: 211 HHEDQEHGHVHHHEDGHGNSITVNLHHHPGTGHHHHDAEEPLLKSDAGCDSTQSGAKDAK 270

Query: 260 SSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSM 319
            +  NIN+  AYLHV+ D IQSIGVMI G ++W+KPEW I+DLICTL+FSV+ L TT  M
Sbjct: 271 KARRNINVHSAYLHVLGDSIQSIGVMIGGAIIWYKPEWKIIDLICTLIFSVIVLFTTIKM 330

Query: 320 LRHIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVC 356
           LR+I  +LME TPRE+   SLEN ++++ GV  +H+LHIW+ITVGKV+L+CHV       
Sbjct: 331 LRNILEVLMESTPREIDATSLENGLRDMDGVVAVHELHIWAITVGKVLLACHVTITQDAD 390

BLAST of PI0018446 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 305.8 bits (782), Expect = 6.2e-82
Identity = 166/345 (48.12%), Postives = 235/345 (68.12%), Query Frame = 0

Query: 20  CCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 79
           C  + C FS  + S++E+ +R+ S  KL   +L   I IVVE++GG++ANSL+++TDAAH
Sbjct: 47  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 106

Query: 80  LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRI 139
           LLSDVA F++SLF++W SGW+A PQ S+G+ R+E+LGALVS+Q+IWL++G+L+YEAI R+
Sbjct: 107 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 166

Query: 140 LAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHS---SHCCHHDH----HSRS 199
                +V+G LMFA++A G ++N+ M I LGH H H H HS    H   HDH     +  
Sbjct: 167 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 226

Query: 200 HQNHLDHERCFLGSK--DNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIV 259
           H +   H+   L     +     N+N+QGAYLHV+ D IQS+GVMI G ++W+KPEW I+
Sbjct: 227 HHHDSGHDESQLSDVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKIL 286

Query: 260 DLICTLVFSVLALATTFSMLRHIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWS 319
           DLICTLVFSV+ L TT  MLR+I  +LME TPRE+    LE  +  I+ V  +H+LHIW+
Sbjct: 287 DLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEVVAVHELHIWA 346

Query: 320 ITVGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNIVHTTIQVE 356
           ITVGK++L+CHV       +  ++ KI  + +  +NI H TIQ+E
Sbjct: 347 ITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIE 391

BLAST of PI0018446 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.5e-75
Identity = 149/337 (44.21%), Postives = 218/337 (64.69%), Query Frame = 0

Query: 20  CCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 79
           C  + C FS    S+ ++ KR+ S  KL  +++   + + +E++ G++ANSL+++ DAAH
Sbjct: 9   CGETACGFST---SSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILADAAH 68

Query: 80  LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRI 139
           LL+DV  F++S+ ++W S WEA P+ S+G+ R+E+LG LVS+QLIWL++G+L+YEA+ R+
Sbjct: 69  LLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEAVTRL 128

Query: 140 LAQ-KTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHSSHCCHHDHHSRSHQNHLD 199
           + +    VDGF M  +AAFG ++N+ M++ LGH H H H H         HS  H  H  
Sbjct: 129 VQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHG--------HSHDH-GHSY 188

Query: 200 HERCFLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVF 259
            ER     + +    NIN+QGAYLHV+ D+IQSIGVMI G ++W+ P+W ++DLICTL F
Sbjct: 189 GERAEQLLEKSKEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFF 248

Query: 260 SVLALATTFSMLRHIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVL 319
           SV+ L TT  MLR I  +LME TPRE+    LE  +  I+ V D+H+LHIW+ITVGK + 
Sbjct: 249 SVIVLGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALF 308

Query: 320 SCHVVAEAGVCSRELILKIKSHCENRYNIVHTTIQVE 356
           SCHV          ++ K+  +    Y I H TIQ+E
Sbjct: 309 SCHVKVRPEAGDEMVLNKVIDYIWREYRISHVTIQIE 333

BLAST of PI0018446 vs. ExPASy TrEMBL
Match: A0A1S3C537 (metal tolerance protein B OS=Cucumis melo OX=3656 GN=LOC103497126 PE=4 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.0e-184
Identity = 344/372 (92.47%), Postives = 348/372 (93.55%), Query Frame = 0

Query: 1   MPIVAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVV 60
           MPIVAKKRNVVIP SSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGG+ILFYTIAIVV
Sbjct: 19  MPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGMILFYTIAIVV 78

Query: 61  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 120
           EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS
Sbjct: 79  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 138

Query: 121 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHS 180
           VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFA+AAFGFILNLFMVIWLGHSHHHHHSHS
Sbjct: 139 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNLFMVIWLGHSHHHHHSHS 198

Query: 181 SHCCHHDHHSRSH--QNHLDHER---------------CFLGSKDNSSTLNINLQGAYLH 240
           SHCCHHDHHS SH  QNHL HE                  LGSKDNSSTLNINLQGAYLH
Sbjct: 199 SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLGSKDNSSTLNINLQGAYLH 258

Query: 241 VITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 300
           VITDMIQSIGVMIAGFVLWFKPEWI+VDLICTLVFSVLALATTFSMLRHIAVILMEGTPR
Sbjct: 259 VITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 318

Query: 301 EVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEN 356
           EVHIESLEN+IKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE 
Sbjct: 319 EVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEK 378

BLAST of PI0018446 vs. ExPASy TrEMBL
Match: A0A5D3BGQ9 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001790 PE=4 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.0e-184
Identity = 344/372 (92.47%), Postives = 348/372 (93.55%), Query Frame = 0

Query: 1   MPIVAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVV 60
           MPIVAKKRNVVIP SSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGG+ILFYTIAIVV
Sbjct: 19  MPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGMILFYTIAIVV 78

Query: 61  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 120
           EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS
Sbjct: 79  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 138

Query: 121 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHS 180
           VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFA+AAFGFILNLFMVIWLGHSHHHHHSHS
Sbjct: 139 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNLFMVIWLGHSHHHHHSHS 198

Query: 181 SHCCHHDHHSRSH--QNHLDHER---------------CFLGSKDNSSTLNINLQGAYLH 240
           SHCCHHDHHS SH  QNHL HE                  LGSKDNSSTLNINLQGAYLH
Sbjct: 199 SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLGSKDNSSTLNINLQGAYLH 258

Query: 241 VITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 300
           VITDMIQSIGVMIAGFVLWFKPEWI+VDLICTLVFSVLALATTFSMLRHIAVILMEGTPR
Sbjct: 259 VITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 318

Query: 301 EVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEN 356
           EVHIESLEN+IKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE 
Sbjct: 319 EVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEK 378

BLAST of PI0018446 vs. ExPASy TrEMBL
Match: A0A5A7SNE8 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00010 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 3.8e-183
Identity = 341/372 (91.67%), Postives = 346/372 (93.01%), Query Frame = 0

Query: 1   MPIVAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVV 60
           MPIVAKKRNVVIP SSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGG+ILFYTIAIVV
Sbjct: 19  MPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGMILFYTIAIVV 78

Query: 61  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 120
           EIIGG RANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE TPQHSFGYNRLEVLGALVS
Sbjct: 79  EIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEVTPQHSFGYNRLEVLGALVS 138

Query: 121 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHS 180
           VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFA+AAFGFILNLFMVIWLGHSHHHHHSHS
Sbjct: 139 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNLFMVIWLGHSHHHHHSHS 198

Query: 181 SHCCHHDHHSRSH--QNHLDHER---------------CFLGSKDNSSTLNINLQGAYLH 240
           SHCCHHDHHS SH  QNHL HE                  LGSKDNSSTLNINLQGAYLH
Sbjct: 199 SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLGSKDNSSTLNINLQGAYLH 258

Query: 241 VITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 300
           VITD+IQSIGVMIAGFVLWFKPEWI+VDLICTLVFSVLALATTFSMLRHIAVILMEGTPR
Sbjct: 259 VITDLIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 318

Query: 301 EVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEN 356
           EVHIESLEN+IKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE 
Sbjct: 319 EVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEK 378

BLAST of PI0018446 vs. ExPASy TrEMBL
Match: I1ZI47 (Metal transport protein 4 OS=Cucumis sativus OX=3659 GN=MTP4 PE=2 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.1e-179
Identity = 334/368 (90.76%), Postives = 344/368 (93.48%), Query Frame = 0

Query: 1   MPIVAKKRNVVIP--ISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI 60
           +PIVAKK N VIP   SSEVKCC+SGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI
Sbjct: 19  IPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI 78

Query: 61  VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL 120
           VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL
Sbjct: 79  VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL 138

Query: 121 VSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHS 180
           VSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFA+AAFGF+LNLFMVIWLGHSHHHHHS
Sbjct: 139 VSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSHHHHHS 198

Query: 181 HSSHCCHHDHHSRSHQNHLDHER-----------CFLGSKDNSSTLNINLQGAYLHVITD 240
           HSSHCCHHDHHS SHQNHL+HE              LGSKDNSSTLNINLQGAYLHVITD
Sbjct: 199 HSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITD 258

Query: 241 MIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPREVHI 300
           MIQSIGVMIAG VLWFKPEWI+VDLICTLVFSVLALATTFSMLRH AVILMEGTPREVHI
Sbjct: 259 MIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHI 318

Query: 301 ESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNI 356
           ESLEN+IKN+KGV+DLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE RYNI
Sbjct: 319 ESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNI 378

BLAST of PI0018446 vs. ExPASy TrEMBL
Match: A0A0A0M0L3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525270 PE=4 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.3e-179
Identity = 333/368 (90.49%), Postives = 343/368 (93.21%), Query Frame = 0

Query: 1   MPIVAKKRNVVIP--ISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI 60
           +PIVAKK N VIP   SSEVKCC+SGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI
Sbjct: 19  IPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI 78

Query: 61  VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL 120
           VVEIIGG RANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL
Sbjct: 79  VVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL 138

Query: 121 VSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHS 180
           VSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFA+AAFGF+LNLFMVIWLGHSHHHHHS
Sbjct: 139 VSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSHHHHHS 198

Query: 181 HSSHCCHHDHHSRSHQNHLDHER-----------CFLGSKDNSSTLNINLQGAYLHVITD 240
           HSSHCCHHDHHS SHQNHL+HE              LGSKDNSSTLNINLQGAYLHVITD
Sbjct: 199 HSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITD 258

Query: 241 MIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPREVHI 300
           MIQSIGVMIAG VLWFKPEWI+VDLICTLVFSVLALATTFSMLRH AVILMEGTPREVHI
Sbjct: 259 MIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHI 318

Query: 301 ESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNI 356
           ESLEN+IKN+KGV+DLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE RYNI
Sbjct: 319 ESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNI 378

BLAST of PI0018446 vs. NCBI nr
Match: TYJ97368.1 (metal tolerance protein B [Cucumis melo var. makuwa])

HSP 1 Score: 654.8 bits (1688), Expect = 4.2e-184
Identity = 344/372 (92.47%), Postives = 348/372 (93.55%), Query Frame = 0

Query: 1   MPIVAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVV 60
           MPIVAKKRNVVIP SSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGG+ILFYTIAIVV
Sbjct: 19  MPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGMILFYTIAIVV 78

Query: 61  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 120
           EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS
Sbjct: 79  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 138

Query: 121 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHS 180
           VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFA+AAFGFILNLFMVIWLGHSHHHHHSHS
Sbjct: 139 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNLFMVIWLGHSHHHHHSHS 198

Query: 181 SHCCHHDHHSRSH--QNHLDHER---------------CFLGSKDNSSTLNINLQGAYLH 240
           SHCCHHDHHS SH  QNHL HE                  LGSKDNSSTLNINLQGAYLH
Sbjct: 199 SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLGSKDNSSTLNINLQGAYLH 258

Query: 241 VITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 300
           VITDMIQSIGVMIAGFVLWFKPEWI+VDLICTLVFSVLALATTFSMLRHIAVILMEGTPR
Sbjct: 259 VITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 318

Query: 301 EVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEN 356
           EVHIESLEN+IKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE 
Sbjct: 319 EVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEK 378

BLAST of PI0018446 vs. NCBI nr
Match: XP_008457438.1 (PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457439.1 PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457440.1 PREDICTED: metal tolerance protein B [Cucumis melo])

HSP 1 Score: 654.8 bits (1688), Expect = 4.2e-184
Identity = 344/372 (92.47%), Postives = 348/372 (93.55%), Query Frame = 0

Query: 1   MPIVAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVV 60
           MPIVAKKRNVVIP SSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGG+ILFYTIAIVV
Sbjct: 19  MPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGMILFYTIAIVV 78

Query: 61  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 120
           EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS
Sbjct: 79  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 138

Query: 121 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHS 180
           VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFA+AAFGFILNLFMVIWLGHSHHHHHSHS
Sbjct: 139 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNLFMVIWLGHSHHHHHSHS 198

Query: 181 SHCCHHDHHSRSH--QNHLDHER---------------CFLGSKDNSSTLNINLQGAYLH 240
           SHCCHHDHHS SH  QNHL HE                  LGSKDNSSTLNINLQGAYLH
Sbjct: 199 SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLGSKDNSSTLNINLQGAYLH 258

Query: 241 VITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 300
           VITDMIQSIGVMIAGFVLWFKPEWI+VDLICTLVFSVLALATTFSMLRHIAVILMEGTPR
Sbjct: 259 VITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 318

Query: 301 EVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEN 356
           EVHIESLEN+IKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE 
Sbjct: 319 EVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEK 378

BLAST of PI0018446 vs. NCBI nr
Match: KAA0031763.1 (metal tolerance protein B [Cucumis melo var. makuwa])

HSP 1 Score: 650.6 bits (1677), Expect = 7.9e-183
Identity = 341/372 (91.67%), Postives = 346/372 (93.01%), Query Frame = 0

Query: 1   MPIVAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVV 60
           MPIVAKKRNVVIP SSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGG+ILFYTIAIVV
Sbjct: 19  MPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGMILFYTIAIVV 78

Query: 61  EIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVS 120
           EIIGG RANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWE TPQHSFGYNRLEVLGALVS
Sbjct: 79  EIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEVTPQHSFGYNRLEVLGALVS 138

Query: 121 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHS 180
           VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFA+AAFGFILNLFMVIWLGHSHHHHHSHS
Sbjct: 139 VQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNLFMVIWLGHSHHHHHSHS 198

Query: 181 SHCCHHDHHSRSH--QNHLDHER---------------CFLGSKDNSSTLNINLQGAYLH 240
           SHCCHHDHHS SH  QNHL HE                  LGSKDNSSTLNINLQGAYLH
Sbjct: 199 SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLGSKDNSSTLNINLQGAYLH 258

Query: 241 VITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 300
           VITD+IQSIGVMIAGFVLWFKPEWI+VDLICTLVFSVLALATTFSMLRHIAVILMEGTPR
Sbjct: 259 VITDLIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHIAVILMEGTPR 318

Query: 301 EVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEN 356
           EVHIESLEN+IKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE 
Sbjct: 319 EVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEK 378

BLAST of PI0018446 vs. NCBI nr
Match: NP_001295861.1 (metal tolerance protein B [Cucumis sativus] >XP_004145281.2 metal tolerance protein B isoform X1 [Cucumis sativus] >XP_011658317.2 metal tolerance protein B isoform X1 [Cucumis sativus] >AFJ24701.1 metal transport protein 4 [Cucumis sativus] >KAE8653262.1 hypothetical protein Csa_019821 [Cucumis sativus])

HSP 1 Score: 639.0 bits (1647), Expect = 2.4e-179
Identity = 334/368 (90.76%), Postives = 344/368 (93.48%), Query Frame = 0

Query: 1   MPIVAKKRNVVIP--ISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI 60
           +PIVAKK N VIP   SSEVKCC+SGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI
Sbjct: 19  IPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI 78

Query: 61  VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL 120
           VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL
Sbjct: 79  VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL 138

Query: 121 VSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHS 180
           VSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFA+AAFGF+LNLFMVIWLGHSHHHHHS
Sbjct: 139 VSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHSHHHHHS 198

Query: 181 HSSHCCHHDHHSRSHQNHLDHER-----------CFLGSKDNSSTLNINLQGAYLHVITD 240
           HSSHCCHHDHHS SHQNHL+HE              LGSKDNSSTLNINLQGAYLHVITD
Sbjct: 199 HSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITD 258

Query: 241 MIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPREVHI 300
           MIQSIGVMIAG VLWFKPEWI+VDLICTLVFSVLALATTFSMLRH AVILMEGTPREVHI
Sbjct: 259 MIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHI 318

Query: 301 ESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNI 356
           ESLEN+IKN+KGV+DLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE RYNI
Sbjct: 319 ESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNI 378

BLAST of PI0018446 vs. NCBI nr
Match: XP_031741134.1 (metal tolerance protein B-like [Cucumis sativus] >XP_031741135.1 metal tolerance protein B-like [Cucumis sativus] >KAE8653268.1 hypothetical protein Csa_023325 [Cucumis sativus])

HSP 1 Score: 632.1 bits (1629), Expect = 2.9e-177
Identity = 333/368 (90.49%), Postives = 343/368 (93.21%), Query Frame = 0

Query: 1   MPIVAKKRNVVIP--ISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI 60
           +PIVAKK N VIP   SSEVKCC+SGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI
Sbjct: 19  IPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAI 78

Query: 61  VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL 120
           VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL
Sbjct: 79  VVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGAL 138

Query: 121 VSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHS 180
           VSVQLIWLISG+LIYEAIDRILA KTKVDGFLMFA+AAFGF+LNLFMVIWLGHS HHHHS
Sbjct: 139 VSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLLNLFMVIWLGHS-HHHHS 198

Query: 181 HSSHCCHHDHHSRSHQNHLDHER-----------CFLGSKDNSSTLNINLQGAYLHVITD 240
           HSSHCCHHDHHS SHQNHL+HE              LGSKDNSSTLNINLQGAYLHVITD
Sbjct: 199 HSSHCCHHDHHSHSHQNHLEHEEEEVYTLTKQEGASLGSKDNSSTLNINLQGAYLHVITD 258

Query: 241 MIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLRHIAVILMEGTPREVHI 300
           MIQSIGVMIAG VLWFKPEWI+VDLICTLVFSVLALATTFSMLRH AVILMEGTPREVHI
Sbjct: 259 MIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALATTFSMLRHTAVILMEGTPREVHI 318

Query: 301 ESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNI 356
           ESLEN+IKN+KGV+DLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCE RYNI
Sbjct: 319 ESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAEAGVCSRELILKIKSHCEKRYNI 378

BLAST of PI0018446 vs. TAIR 10
Match: AT2G29410.1 (metal tolerance protein B1 )

HSP 1 Score: 352.4 bits (903), Expect = 4.1e-97
Identity = 182/343 (53.06%), Postives = 242/343 (70.55%), Query Frame = 0

Query: 25  CAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDV 84
           CAF+R EH   E+ +R +S  +L  LI  Y I + V+I+GG +ANSL+VMTDAAHLLSDV
Sbjct: 36  CAFTRQEHCVSETKEREESTRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDV 95

Query: 85  AGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKT 144
           AG  VSL A+ VS WEA P++SFG+ RLEVL A +SVQLIWL+SGV+I+EAI R+L++  
Sbjct: 96  AGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSR 155

Query: 145 KVDGFLMFAIAAFGFILNLFMVIWLGHSH-HHHHSHSSHCCHHDHHSRSHQNHLDHERC- 204
           +V+G +MF I+AFGF +NL MV+WLGH+H HHHH H     HH HH+  HQ+   H+   
Sbjct: 156 EVNGEIMFGISAFGFFMNLVMVLWLGHNHSHHHHDH-----HHHHHNHKHQHQHHHKEVV 215

Query: 205 ----------FLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIVDL 264
                       G K +S  +NIN+QGAYLH + DMIQS+GVMI G ++W KP+W++VDL
Sbjct: 216 AEEEEEEMNPLKGEKSSSKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDL 275

Query: 265 ICTLVFSVLALATTFSMLRHIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWSIT 324
           ICTLVFS  ALA T  +L++I  ILME  PR++ IE LE  +K I GV  ++DLH+W IT
Sbjct: 276 ICTLVFSAFALAATLPILKNIFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEIT 335

Query: 325 VGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNIVHTTIQVE 356
           VG++VLSCH++ E G   +E+I  +++ C   Y I H T+QVE
Sbjct: 336 VGRIVLSCHILPEPGASPKEIITGVRNFCRKSYGIYHATVQVE 373

BLAST of PI0018446 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 312.0 bits (798), Expect = 6.2e-85
Identity = 174/384 (45.31%), Postives = 249/384 (64.84%), Query Frame = 0

Query: 4   VAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEII 63
           V K     I ++S+V C  + C FS  ++++ ++ +RS S  KL   ++   + + VE++
Sbjct: 16  VGKSDEERIIVASKV-CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVV 75

Query: 64  GGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQL 123
           GG++ANSL+++TDAAHLLSDVA F++SLF++W +GWEATP+ ++G+ R+E+LGALVS+QL
Sbjct: 76  GGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQL 135

Query: 124 IWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHSSHC 183
           IWL++G+L+YEAI RI+ + ++V+GFLMF +AAFG ++N+ M + LGH H H H H  H 
Sbjct: 136 IWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGH-GHG 195

Query: 184 CHHDHHSRS-------HQNHLDHERCFLGSK----------------DNSST-------- 243
             HDHH+ S       H +H DHE                       D S T        
Sbjct: 196 HGHDHHNHSHGVTVTTHHHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKR 255

Query: 244 -LNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLR 303
             NINLQGAYLHV+ D IQS+GVMI G ++W+ PEW IVDLICTL FSV+ L TT +M+R
Sbjct: 256 KRNINLQGAYLHVLGDSIQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIR 315

Query: 304 HIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSR 356
           +I  +LME TPRE+    LE  +  ++ V  +H+LHIW+ITVGKV+L+CHV       + 
Sbjct: 316 NILEVLMESTPREIDATKLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADAD 375

BLAST of PI0018446 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 312.0 bits (798), Expect = 6.2e-85
Identity = 174/384 (45.31%), Postives = 249/384 (64.84%), Query Frame = 0

Query: 4   VAKKRNVVIPISSEVKCCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEII 63
           V K     I ++S+V C  + C FS  ++++ ++ +RS S  KL   ++   + + VE++
Sbjct: 16  VGKSDEERIIVASKV-CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVV 75

Query: 64  GGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQL 123
           GG++ANSL+++TDAAHLLSDVA F++SLF++W +GWEATP+ ++G+ R+E+LGALVS+QL
Sbjct: 76  GGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQL 135

Query: 124 IWLISGVLIYEAIDRILAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHSSHC 183
           IWL++G+L+YEAI RI+ + ++V+GFLMF +AAFG ++N+ M + LGH H H H H  H 
Sbjct: 136 IWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGH-GHG 195

Query: 184 CHHDHHSRS-------HQNHLDHERCFLGSK----------------DNSST-------- 243
             HDHH+ S       H +H DHE                       D S T        
Sbjct: 196 HGHDHHNHSHGVTVTTHHHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKR 255

Query: 244 -LNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIVDLICTLVFSVLALATTFSMLR 303
             NINLQGAYLHV+ D IQS+GVMI G ++W+ PEW IVDLICTL FSV+ L TT +M+R
Sbjct: 256 KRNINLQGAYLHVLGDSIQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIR 315

Query: 304 HIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAEAGVCSR 356
           +I  +LME TPRE+    LE  +  ++ V  +H+LHIW+ITVGKV+L+CHV       + 
Sbjct: 316 NILEVLMESTPREIDATKLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADAD 375

BLAST of PI0018446 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 305.8 bits (782), Expect = 4.4e-83
Identity = 166/345 (48.12%), Postives = 235/345 (68.12%), Query Frame = 0

Query: 20  CCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 79
           C  + C FS  + S++E+ +R+ S  KL   +L   I IVVE++GG++ANSL+++TDAAH
Sbjct: 86  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 145

Query: 80  LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRI 139
           LLSDVA F++SLF++W SGW+A PQ S+G+ R+E+LGALVS+Q+IWL++G+L+YEAI R+
Sbjct: 146 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 205

Query: 140 LAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHS---SHCCHHDH----HSRS 199
                +V+G LMFA++A G ++N+ M I LGH H H H HS    H   HDH     +  
Sbjct: 206 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 265

Query: 200 HQNHLDHERCFLGSK--DNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIV 259
           H +   H+   L     +     N+N+QGAYLHV+ D IQS+GVMI G ++W+KPEW I+
Sbjct: 266 HHHDSGHDESQLSDVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKIL 325

Query: 260 DLICTLVFSVLALATTFSMLRHIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWS 319
           DLICTLVFSV+ L TT  MLR+I  +LME TPRE+    LE  +  I+ V  +H+LHIW+
Sbjct: 326 DLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEVVAVHELHIWA 385

Query: 320 ITVGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNIVHTTIQVE 356
           ITVGK++L+CHV       +  ++ KI  + +  +NI H TIQ+E
Sbjct: 386 ITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIE 430

BLAST of PI0018446 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 305.8 bits (782), Expect = 4.4e-83
Identity = 166/345 (48.12%), Postives = 235/345 (68.12%), Query Frame = 0

Query: 20  CCNSGCAFSRLEHSNLESLKRSKSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAH 79
           C  + C FS  + S++E+ +R+ S  KL   +L   I IVVE++GG++ANSL+++TDAAH
Sbjct: 47  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 106

Query: 80  LLSDVAGFSVSLFAVWVSGWEATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRI 139
           LLSDVA F++SLF++W SGW+A PQ S+G+ R+E+LGALVS+Q+IWL++G+L+YEAI R+
Sbjct: 107 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 166

Query: 140 LAQKTKVDGFLMFAIAAFGFILNLFMVIWLGHSHHHHHSHS---SHCCHHDH----HSRS 199
                +V+G LMFA++A G ++N+ M I LGH H H H HS    H   HDH     +  
Sbjct: 167 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 226

Query: 200 HQNHLDHERCFLGSK--DNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIIV 259
           H +   H+   L     +     N+N+QGAYLHV+ D IQS+GVMI G ++W+KPEW I+
Sbjct: 227 HHHDSGHDESQLSDVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKIL 286

Query: 260 DLICTLVFSVLALATTFSMLRHIAVILMEGTPREVHIESLENEIKNIKGVHDLHDLHIWS 319
           DLICTLVFSV+ L TT  MLR+I  +LME TPRE+    LE  +  I+ V  +H+LHIW+
Sbjct: 287 DLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEVVAVHELHIWA 346

Query: 320 ITVGKVVLSCHVVAEAGVCSRELILKIKSHCENRYNIVHTTIQVE 356
           ITVGK++L+CHV       +  ++ KI  + +  +NI H TIQ+E
Sbjct: 347 ITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIE 391

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6DBM85.8e-9653.06Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1[more]
Q9ZT638.7e-8445.31Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2[more]
Q688R19.6e-8344.82Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 ... [more]
Q9LXS16.2e-8248.12Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2[more]
Q9M2711.5e-7544.21Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3C5372.0e-18492.47metal tolerance protein B OS=Cucumis melo OX=3656 GN=LOC103497126 PE=4 SV=1[more]
A0A5D3BGQ92.0e-18492.47Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7SNE83.8e-18391.67Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
I1ZI471.1e-17990.76Metal transport protein 4 OS=Cucumis sativus OX=3659 GN=MTP4 PE=2 SV=1[more]
A0A0A0M0L33.3e-17990.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525270 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
TYJ97368.14.2e-18492.47metal tolerance protein B [Cucumis melo var. makuwa][more]
XP_008457438.14.2e-18492.47PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457439.1 PREDICTED: m... [more]
KAA0031763.17.9e-18391.67metal tolerance protein B [Cucumis melo var. makuwa][more]
NP_001295861.12.4e-17990.76metal tolerance protein B [Cucumis sativus] >XP_004145281.2 metal tolerance prot... [more]
XP_031741134.12.9e-17790.49metal tolerance protein B-like [Cucumis sativus] >XP_031741135.1 metal tolerance... [more]
Match NameE-valueIdentityDescription
AT2G29410.14.1e-9753.06metal tolerance protein B1 [more]
AT2G46800.16.2e-8545.31zinc transporter of Arabidopsis thaliana [more]
AT2G46800.26.2e-8545.31zinc transporter of Arabidopsis thaliana [more]
AT3G58810.14.4e-8348.12metal tolerance protein A2 [more]
AT3G58810.24.4e-8348.12metal tolerance protein A2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002524Cation efflux proteinTIGRFAMTIGR01297TIGR01297coord: 51..355
e-value: 9.7E-79
score: 262.5
IPR002524Cation efflux proteinPFAMPF01545Cation_effluxcoord: 51..278
e-value: 2.9E-39
score: 134.9
IPR027469Cation efflux transmembrane domain superfamilyGENE3D1.20.1510.10Cation efflux protein transmembrane domaincoord: 47..277
e-value: 9.3E-47
score: 161.2
IPR027469Cation efflux transmembrane domain superfamilySUPERFAMILY161111Cation efflux protein transmembrane domain-likecoord: 48..279
NoneNo IPR availablePANTHERPTHR11562CATION EFFLUX PROTEIN/ ZINC TRANSPORTERcoord: 4..355
NoneNo IPR availablePANTHERPTHR11562:SF54METAL TOLERANCE PROTEIN Bcoord: 4..355
IPR036837Cation efflux protein, cytoplasmic domain superfamilySUPERFAMILY160240Cation efflux protein cytoplasmic domain-likecoord: 284..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0018446.1PI0018446.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098655 cation transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008324 cation transmembrane transporter activity