PI0018059 (gene) Melon (PI 482460) v1

BLAST of PI0018059 vs. ExPASy Swiss-Prot
Match: Q6PCD5 (E3 ubiquitin-protein ligase RFWD3 OS=Homo sapiens OX=9606 GN=RFWD3 PE=1 SV=3)

HSP 1 Score: 128.3 bits (321), Expect = 3.0e-28
Identity = 87/309 (28.16%), Postives = 152/309 (49.19%), Query Frame = 0

Query: 101 NENDDMICSICMDAWTNYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTK 160
           +E +   C+IC++ WTN G+H +  L CGHL+G  CI+ WL+ +    KCPQCN+K    
Sbjct: 280 DEEEGDTCTICLEQWTNAGDHRLSALRCGHLFGYRCISTWLKGQ--VRKCPQCNKKARHS 339

Query: 161 DVRKIFAPRIVAADGESEKRIKSLEATCASLEKKVQVEWKK----------RETDLQSKL 220
           D+  ++A  + A D   ++R+KS       L K+ ++E  +          + T LQ ++
Sbjct: 340 DIVVLYARTLRALDTSEQERMKSSLLKEQMLRKQAELESAQCRLQLQVLTDKCTRLQRRV 399

Query: 221 RELTERTNYLEHLLEDSQRKPVLANLDC--TFQGKSVSEHNFGLRFS-GQVSSCSFILKK 280
           ++L + T++    L+  +       L C  + QG+   +++F   F+  Q  +C  +   
Sbjct: 400 QDLQKLTSHQSQNLQQPRGSQAWV-LSCSPSSQGQHKHKYHFQKTFTVSQAGNCRIMAYC 459

Query: 281 ELLVDGARLVDVDASNHVVLVARRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDL 340
           + L   + LV    S     +     GFG   + T     S Y      IP     +R L
Sbjct: 460 DAL---SCLVISQPSPQASFL----PGFGVKMLSTANMKSSQY------IPMHGKQIRGL 519

Query: 341 HISPAARNLVLFSSLGKKLAILSMESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGL 397
             S   R L+L +SL   + + S+E+N ++  Y+     WSC W  + ++++YAGL NG 
Sbjct: 520 AFSSYLRGLLLSASLDNTIKLTSLETNTVVQTYNAGRPVWSCCWCLDEANYIYAGLANGS 572

BLAST of PI0018059 vs. ExPASy Swiss-Prot
Match: Q8CIK8 (E3 ubiquitin-protein ligase RFWD3 OS=Mus musculus OX=10090 GN=Rfwd3 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.4e-25
Identity = 101/381 (26.51%), Postives = 171/381 (44.88%), Query Frame = 0

Query: 25  EEEDEEETDDSEETVEGHAEQEVDYVPEVLQSPRASQTGTSGSAQARVSFWVEEEVDKRR 84
           + E       ++   +G AE E   V          + G   + Q   S   ++ V K  
Sbjct: 211 DSETRNPPSGTDSDSDGSAEDEEVVVQAEEPEANIPEQGVIATDQEATSVTGDDAVPKES 270

Query: 85  RTEGGEASSSQNEWNRNENDDMICSICMDAWTNYGEHHICCLPCGHLYGMSCINRWLRQR 144
             +    S+ ++E      +   C+IC++ WTN G+H I  L CGHL+G  CI++WL+ +
Sbjct: 271 PQKPNMLSAMEDE------EGETCTICLEQWTNAGDHRISALRCGHLFGFRCISKWLKGQ 330

Query: 145 KNAGKCPQCNRKCSTKDVRKIFAPRIVAADGESEKRIKSLEATCASLEKKVQVEWKKRET 204
               KCPQCN+K    D+  I+A  + A D   ++R+KS       L K+ ++E  +   
Sbjct: 331 TR--KCPQCNKKAKHSDIVVIYARSLRALDTSEQERMKSDLLNEQMLRKQAELESAQCRL 390

Query: 205 DLQSKLRELTERTNYLEHLLEDSQRKPVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFI 264
            LQ  + + T+  + ++ L      K V+ + +   Q  S S+   G   S   S   + 
Sbjct: 391 QLQVLIDKCTKLNSRVQDL-----EKFVVRHQNSAAQQCSRSKALCGKCLSSSQSPGKYS 450

Query: 265 LKKELLVD--GARLVDVDASNHVVLVARRQS-------GFGGTHVLTKISLMSPYEREDI 324
            +K   +   G   +         LVA + S       GFG   + T     S Y     
Sbjct: 451 SEKTFTISQTGKCRILAYCDGLSCLVASQPSPQASFLPGFGVKMLSTANMKSSQY----- 510

Query: 325 GIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILSMESNHIILDYDLPDAAWSCSWDQNS 384
            IP     +R L  S  ++ L+L +SL   + + S+E+N ++  Y+     WSC W  + 
Sbjct: 511 -IPMHNKQIRGLSFSSQSKGLLLSASLDCTIKLTSLETNTVVQTYNTGRPVWSCCWCLDE 570

Query: 385 SHHVYAGLKNGLLVIFDMRQT 397
           +++VYAGL +G ++I+D+R T
Sbjct: 571 NNYVYAGLASGSILIYDLRNT 572

BLAST of PI0018059 vs. ExPASy Swiss-Prot
Match: D2HWM5 (E3 ubiquitin-protein ligase RFWD3 OS=Ailuropoda melanoleuca OX=9646 GN=RFWD3 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.3e-23
Identity = 113/477 (23.69%), Postives = 207/477 (43.40%), Query Frame = 0

Query: 17  EDGGEEEEEEEDEEETDDSEETVEGHAE-QEVDYVPEVLQSPRASQTGTSGSAQARVSFW 76
           +D        ED + +  S    E  AE +EV    E  ++  + + G + + Q      
Sbjct: 198 QDSETRNPVSEDLQVSSSSSSDSESSAEYEEVVVQAEDTRAVVSEEQGGTAAEQEVTCVG 257

Query: 77  VEEEVDKRRRTEGGEASSSQNEWNRNENDDMICSICMDAWTNYGEHHICCLPCGHLYGMS 136
             E + K+   +      S    ++++ +   C+IC + WTN G+H +  L CGHL+G  
Sbjct: 258 GGETLPKQSPQKTNPLLPSV---SKDDEEGDTCTICFEHWTNAGDHRLSALRCGHLFGYK 317

Query: 137 CINRWLRQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGESEKRIKSLEATCASLEKKV 196
           CI++WL+ +  A KCPQCN+K    D+  ++A  + A D    +R+KS       L K+ 
Sbjct: 318 CISKWLKGQ--ARKCPQCNKKAKHSDIVVLYARTLRALDTSEHERMKSSLLKEQMLRKQA 377

Query: 197 QVEWKKRETDLQ----------SKLRELTERTNYLEHLLEDSQRKPVLANLDCTFQGKSV 256
           ++E  +    LQ          S++++L + T      +  S       +L+C    ++ 
Sbjct: 378 ELESAQCRLQLQVLTDECSKLHSRVQDLQKLTVQHRDQISQSPSGSQARSLNCLPSSQNQ 437

Query: 257 SEHNFGLRFS-GQVSSCSFILKKELLVDGARLVDVDASNHVVLVARRQSGFGGTHVLTKI 316
            +++F   F+     +C  +   + L   + LV    S     +     GFG   + T  
Sbjct: 438 RKYHFQKTFTVSPTGNCRIMTYCDAL---SCLVVSQPSPQASFL----PGFGVKMLSTAN 497

Query: 317 SLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILSMESNHIILDYDLPD 376
              S Y      +P     +R L  S  ++ L+L +SL   + + S+E+N ++  Y+   
Sbjct: 498 MKSSQY------VPMHGKQIRGLAFSSRSKGLLLSASLDSTVKLTSLETNTVVQTYNAGR 557

Query: 377 AAWSCSWDQNSSHHVYAGLKNGLLVIFDMRQTVGPL-KILQGLTNNPIHTIHSL-SSYST 436
             WSC W  + S+H+YAGL NG ++++D+R T   + +++      P+ ++  +  + S 
Sbjct: 558 PVWSCCWCLDESNHIYAGLVNGSILVYDLRNTSSHIQELVPQKARCPLVSLSYIPRAASA 617

Query: 437 VSNDKSVLSASAYGICQWNFD-ALEEGPFLIPETNQGVCTSLAYCSSRDELVASYRP 479
                 VL+ +      W         P ++P    G C      SS    + +YRP
Sbjct: 618 AFPYGGVLAGTLENASFWELKMGFSHWPHVLP-MEPGGCVDFQTESSTRHCLVTYRP 655

BLAST of PI0018059 vs. ExPASy TrEMBL
Match: A0A1S3CH35 (E3 ubiquitin-protein ligase RFWD3 OS=Cucumis melo OX=3656 GN=LOC103500825 PE=4 SV=1)

HSP 1 Score: 1155.6 bits (2988), Expect = 0.0e+00
Identity = 578/620 (93.23%), Postives = 597/620 (96.29%), Query Frame = 0

Query: 1   MPDSDILHVHYEVEDVEDGGEEEEEEE-----DEEETDDSEETVEGHAEQEVDYVPEVLQ 60
           MPDSDILHVHYEVEDV+DGG EEEEEE     D+EETD+SEETVEGHAE+ VDYVPEVLQ
Sbjct: 1   MPDSDILHVHYEVEDVDDGGGEEEEEEEEDDDDDEETDESEETVEGHAEEGVDYVPEVLQ 60

Query: 61  SPRASQTGTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNRNENDDMICSICMDAW 120
           SPRASQTGT+GSAQARVSFWVEEE+DKRRRTEGGEASSSQNEWNRNENDDMICSICMDAW
Sbjct: 61  SPRASQTGTTGSAQARVSFWVEEELDKRRRTEGGEASSSQNEWNRNENDDMICSICMDAW 120

Query: 121 TNYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADG 180
           TNYGEHHICCLPCGHLYGMSCINRWL+QRKNAGKCPQCNRKCSTKDVRK+FAPRIVAADG
Sbjct: 121 TNYGEHHICCLPCGHLYGMSCINRWLQQRKNAGKCPQCNRKCSTKDVRKLFAPRIVAADG 180

Query: 181 ESEKRIKSLEATCASLEKK------VQVEWKKRETDLQSKLRELTERTNYLEHLLEDSQR 240
           ES+KRIKSLEATCASLEKK       +VEWKKRETDLQ KL+EL+ERTNYLEHLLEDSQR
Sbjct: 181 ESQKRIKSLEATCASLEKKDANWCRKEVEWKKRETDLQLKLQELSERTNYLEHLLEDSQR 240

Query: 241 KPVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLVDVDASNHVVLVA 300
           KPVLANLDCTFQGKSVSEHNFGLRFS QVSSCSFILKKEL+VDGARL+DVDASN +VLVA
Sbjct: 241 KPVLANLDCTFQGKSVSEHNFGLRFSSQVSSCSFILKKELMVDGARLIDVDASNQIVLVA 300

Query: 301 RRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAIL 360
           RRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAIL
Sbjct: 301 RRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAIL 360

Query: 361 SMESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMRQTVGPLKILQGLTNN 420
           SMESNHIILDYDLP+AAWSCSWDQNSSHHVYAGLKNGLLV+FDMRQTVGPLKILQGLTNN
Sbjct: 361 SMESNHIILDYDLPEAAWSCSWDQNSSHHVYAGLKNGLLVMFDMRQTVGPLKILQGLTNN 420

Query: 421 PIHTIHSLSSYSTVSNDKSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSR 480
           PIHTIHSLSSYSTVSNDKS+LSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSR
Sbjct: 421 PIHTIHSLSSYSTVSNDKSILSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSR 480

Query: 481 DELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKED-HQYSKLSSAYANID 540
           DELVASYRPKVGMSNEIVYSQPSPSPSHT GHRVDGCHVAFKKED HQ+SKL SAYANID
Sbjct: 481 DELVASYRPKVGMSNEIVYSQPSPSPSHTTGHRVDGCHVAFKKEDHHQFSKLGSAYANID 540

Query: 541 AIRLPKSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHLKVRKHPVRDVKYSYGFD 600
           AIRLPKSTIIDLQ CNSLFVSADAVMGDLILQELPSFRSVQHLKV KHPVRDVKYSYGFD
Sbjct: 541 AIRLPKSTIIDLQGCNSLFVSADAVMGDLILQELPSFRSVQHLKVHKHPVRDVKYSYGFD 600

Query: 601 GGLLSCLSDDILHLFCTKGS 609
            GLLSCLSDDILHLFCTKGS
Sbjct: 601 RGLLSCLSDDILHLFCTKGS 620

BLAST of PI0018059 vs. ExPASy TrEMBL
Match: A0A0A0KER3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G446500 PE=4 SV=1)

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 580/619 (93.70%), Postives = 598/619 (96.61%), Query Frame = 0

Query: 1   MPDSDILHVHYEVEDVEDGG----EEEEEEEDEEETDDSEETVEGHAEQEVDYVPEVLQS 60
           MPDSDILHVHYEVEDV+DGG    +E+EEEE+EEETDDSEETVEGHAE+EVDYVPEVLQS
Sbjct: 1   MPDSDILHVHYEVEDVDDGGGEEEQEQEEEEEEEETDDSEETVEGHAEEEVDYVPEVLQS 60

Query: 61  PRASQTGTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNRNENDDMICSICMDAWT 120
           PRASQTGT GSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNR+ENDDMICSICMDAWT
Sbjct: 61  PRASQTGTPGSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNRSENDDMICSICMDAWT 120

Query: 121 NYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGE 180
           NYGEHHICCLPCGHLYGMSCINRWL+QRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGE
Sbjct: 121 NYGEHHICCLPCGHLYGMSCINRWLQQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGE 180

Query: 181 SEKRIKSLEATCASLEK------KVQVEWKKRETDLQSKLRELTERTNYLEHLLEDSQRK 240
           S+KRIKSLEATCASLEK      K +VEWKKRETD+QSKLREL+ERTNYLEHLLEDSQRK
Sbjct: 181 SQKRIKSLEATCASLEKKEANWCKKEVEWKKRETDMQSKLRELSERTNYLEHLLEDSQRK 240

Query: 241 PVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLVDVDASNHVVLVAR 300
           PVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARL+DVDASN +VLVAR
Sbjct: 241 PVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLIDVDASNQIVLVAR 300

Query: 301 RQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILS 360
           R  GFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILS
Sbjct: 301 R--GFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILS 360

Query: 361 MESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMRQTVGPLKILQGLTNNP 420
           MESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLV+FDMRQTVGPLKILQGLTNNP
Sbjct: 361 MESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVMFDMRQTVGPLKILQGLTNNP 420

Query: 421 IHTIHSLSSYSTVSNDKSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSRD 480
           IHTIHSLSS+STVSNDKSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSRD
Sbjct: 421 IHTIHSLSSHSTVSNDKSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSRD 480

Query: 481 ELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKED-HQYSKLSSAYANIDA 540
           ELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKED HQ+SKL SA+ANIDA
Sbjct: 481 ELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKEDHHQFSKLGSAHANIDA 540

Query: 541 IRLPKSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHLKVRKHPVRDVKYSYGFDG 600
           IRLPKSTIIDL DCNSLFVSADA+MGDLILQELPSFRSVQHLKV KHPVRDVKYSYGFDG
Sbjct: 541 IRLPKSTIIDLPDCNSLFVSADAIMGDLILQELPSFRSVQHLKVHKHPVRDVKYSYGFDG 600

Query: 601 GLLSCLSDDILHLFCTKGS 609
           GLL CLSDDIL LFCTKGS
Sbjct: 601 GLLGCLSDDILQLFCTKGS 617

BLAST of PI0018059 vs. ExPASy TrEMBL
Match: A0A6J1HDN7 (E3 ubiquitin-protein ligase RFWD3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463210 PE=4 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 2.0e-280
Identity = 502/637 (78.81%), Postives = 551/637 (86.50%), Query Frame = 0

Query: 1   MPDS-----DILHV-----HYEVEDVEDGGEEEEEEEDEEETDDSEETVEGHAEQEVDYV 60
           MPDS     DI +V      YEVED +DG  EEEEE+D+EETDD EETVEGHAE+  D V
Sbjct: 1   MPDSFPSYVDIQNVLVDGEQYEVEDSDDG--EEEEEDDDEETDDGEETVEGHAEENADNV 60

Query: 61  PEVLQSPRASQTGTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQ----------NEWNR 120
            E  QSPRASQTG S S QARVSFWVEEE DKRRRTEGGE SSS           NEWNR
Sbjct: 61  IEA-QSPRASQTGPSRSGQARVSFWVEEEEDKRRRTEGGEGSSSNGVGSVEISQGNEWNR 120

Query: 121 NENDDMICSICMDAWTNYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTK 180
           NE D + C ICM+AWTNYGEHHICCLPCGHLYGMSCINRWL+QR+ AGKCPQCNRKCS K
Sbjct: 121 NEIDGLFCPICMEAWTNYGEHHICCLPCGHLYGMSCINRWLQQRRKAGKCPQCNRKCSMK 180

Query: 181 DVRKIFAPRIVAADGESEKRIKSLEATCASLEK------KVQVEWKKRETDLQSKLRELT 240
           DVRK+FA RIVAAD ES+KRI+SLEATCASLE       K +VEWKK+ETDLQSK+ ELT
Sbjct: 181 DVRKLFASRIVAADEESQKRIQSLEATCASLETKNANWCKKEVEWKKKETDLQSKVHELT 240

Query: 241 ERTNYLEHLLEDSQRKPVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGA 300
           ERTNYLEHLLED++RK  +A+LDC F+GKSVS+HNFGLRFSGQVSSC FIL+KELLVDGA
Sbjct: 241 ERTNYLEHLLEDTKRKHEVAHLDCIFEGKSVSDHNFGLRFSGQVSSCGFILEKELLVDGA 300

Query: 301 RLVDVDASNHVVLVARRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAAR 360
           R +DVD+SN VVLVARRQSGFGGTHV+TKISLMSPYEREDI IPFCTNAV+DLHISPAAR
Sbjct: 301 RSIDVDSSNQVVLVARRQSGFGGTHVITKISLMSPYEREDIRIPFCTNAVKDLHISPAAR 360

Query: 361 NLVLFSSLGKKLAILSMESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMR 420
            LVL+SSLGKKLA+LSMESN+IILDYDLPDAAWSCSWDQNSSH+VYAGLKNG L++FDMR
Sbjct: 361 KLVLYSSLGKKLAVLSMESNNIILDYDLPDAAWSCSWDQNSSHYVYAGLKNGSLMMFDMR 420

Query: 421 QTVGPLKILQGLTNNPIHTIHSLSSYSTVSND-KSVLSASAYGICQWNFDALEEGPFLIP 480
           QTVGPLK L+G+T+NP+HTIHSLS+  T+SND KSVLSASAYG+CQWNFDA EEGPFL+P
Sbjct: 421 QTVGPLKTLRGITSNPVHTIHSLSNNLTLSNDVKSVLSASAYGVCQWNFDASEEGPFLVP 480

Query: 481 ETNQGVCTSLAYCSSRDELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKE 540
           ETNQGVCTSLAYCSSRDE+VASYRPKVGM +EI YSQ SPSPSHTAGHRVDGCH+AFKKE
Sbjct: 481 ETNQGVCTSLAYCSSRDEIVASYRPKVGMPSEIPYSQISPSPSHTAGHRVDGCHMAFKKE 540

Query: 541 DH--QYSKLSSAYANIDAIRLPKSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHL 600
           ++  ++SKL SA ANIDAIRLPKSTIIDLQDC+SLFV  DAVM +LILQELPSFRS QHL
Sbjct: 541 ENFQKFSKLGSARANIDAIRLPKSTIIDLQDCSSLFVFGDAVMNELILQELPSFRSFQHL 600

Query: 601 KVRKHPVRDVKYSYGFDGGLLSCLSDDILHLFCTKGS 609
           KV KHPVRDVKYSYGF  GLLSCLSDD L L CTKGS
Sbjct: 601 KVHKHPVRDVKYSYGFGRGLLSCLSDDTLQLLCTKGS 634

BLAST of PI0018059 vs. ExPASy TrEMBL
Match: A0A6J1I5M2 (E3 ubiquitin-protein ligase RFWD3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471236 PE=4 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 2.6e-280
Identity = 501/637 (78.65%), Postives = 552/637 (86.66%), Query Frame = 0

Query: 1   MPDS-----DILHV-----HYEVEDVEDGGEEEEEEEDEEETDDSEETVEGHAEQEVDYV 60
           MPDS     DI +V      YEVED +DG  EEEEE+D+EETDD EETVEGHAE+  D V
Sbjct: 1   MPDSFPSYVDIQNVLVDGEQYEVEDSDDG--EEEEEDDDEETDDGEETVEGHAEENADNV 60

Query: 61  PEVLQSPRASQTGTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQ----------NEWNR 120
            E  QSPRASQTG S S QARVSFWVEEE DKRRRT+GGEASSS           NEWNR
Sbjct: 61  IEA-QSPRASQTGPSRSGQARVSFWVEEEEDKRRRTDGGEASSSNGVGSVEISQGNEWNR 120

Query: 121 NENDDMICSICMDAWTNYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTK 180
           NE D + C ICM+AWTNYG+HHICCLPCGHLYGMSCINRWL+QR+ AGKCPQCNRKCS K
Sbjct: 121 NEIDGLFCPICMEAWTNYGDHHICCLPCGHLYGMSCINRWLQQRRKAGKCPQCNRKCSMK 180

Query: 181 DVRKIFAPRIVAADGESEKRIKSLEATCASLEK------KVQVEWKKRETDLQSKLRELT 240
           DVRK+FA RIVAAD ES+KRI+SLEATCASLE       K +VEWKK+ETDLQSK+ ELT
Sbjct: 181 DVRKLFASRIVAADEESQKRIQSLEATCASLETKNANWCKKEVEWKKKETDLQSKVYELT 240

Query: 241 ERTNYLEHLLEDSQRKPVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGA 300
           ERTNYLEHLLED++RK  +A+LDC F+GKSVS+HNFGLRFSGQVSSC FIL+KELLVDGA
Sbjct: 241 ERTNYLEHLLEDTKRKHEVAHLDCIFEGKSVSDHNFGLRFSGQVSSCGFILEKELLVDGA 300

Query: 301 RLVDVDASNHVVLVARRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAAR 360
           R +DVD+SN VVLVARRQ GFGGTHV+TKISLMSPYEREDI IPFCTNAV+DLHISPAAR
Sbjct: 301 RSIDVDSSNQVVLVARRQPGFGGTHVITKISLMSPYEREDIRIPFCTNAVKDLHISPAAR 360

Query: 361 NLVLFSSLGKKLAILSMESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMR 420
           NLVL+SSLGKKLA+LSMESN+IILDYDLPDAAWSCSWDQNSSH+VYAGLKNG L++FDMR
Sbjct: 361 NLVLYSSLGKKLAVLSMESNNIILDYDLPDAAWSCSWDQNSSHYVYAGLKNGSLMMFDMR 420

Query: 421 QTVGPLKILQGLTNNPIHTIHSLSSYSTVSND-KSVLSASAYGICQWNFDALEEGPFLIP 480
           QTVGPLK LQG+T+NP+HTIHSLS+  T+SND KSVLSASAYG+CQWNFDA EEGPFL+P
Sbjct: 421 QTVGPLKTLQGITSNPVHTIHSLSNNLTLSNDVKSVLSASAYGVCQWNFDASEEGPFLVP 480

Query: 481 ETNQGVCTSLAYCSSRDELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKE 540
           ETNQGVCTSLAYCSSRDE+VASYRPKVGMS+EI YSQ SPSPSHTAGHRVDGCH+AFKKE
Sbjct: 481 ETNQGVCTSLAYCSSRDEIVASYRPKVGMSSEIPYSQISPSPSHTAGHRVDGCHMAFKKE 540

Query: 541 DH--QYSKLSSAYANIDAIRLPKSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHL 600
           ++  ++SKL SA ANIDAIRLPKST+IDLQDC+SLFV  DAVM +LILQELPSFRS QHL
Sbjct: 541 ENFQKFSKLGSARANIDAIRLPKSTLIDLQDCSSLFVFGDAVMNELILQELPSFRSFQHL 600

Query: 601 KVRKHPVRDVKYSYGFDGGLLSCLSDDILHLFCTKGS 609
           KV KHPVRDVKYSYGF  GLLSCLSDD L L CT GS
Sbjct: 601 KVHKHPVRDVKYSYGFGRGLLSCLSDDTLQLLCTNGS 634

BLAST of PI0018059 vs. ExPASy TrEMBL
Match: A0A6J1HFZ1 (E3 ubiquitin-protein ligase RFWD3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111463210 PE=4 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 4.4e-264
Identity = 482/637 (75.67%), Postives = 530/637 (83.20%), Query Frame = 0

Query: 1   MPDS-----DILHV-----HYEVEDVEDGGEEEEEEEDEEETDDSEETVEGHAEQEVDYV 60
           MPDS     DI +V      YEVED +DG  EEEEE+D+EETDD EETVEGHAE+  D V
Sbjct: 1   MPDSFPSYVDIQNVLVDGEQYEVEDSDDG--EEEEEDDDEETDDGEETVEGHAEENADNV 60

Query: 61  PEVLQSPRASQTGTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQ----------NEWNR 120
            E  QSPRASQTG S S QARVSFWVEEE DKRRRTEGGE SSS           NEWNR
Sbjct: 61  IEA-QSPRASQTGPSRSGQARVSFWVEEEEDKRRRTEGGEGSSSNGVGSVEISQGNEWNR 120

Query: 121 NENDDMICSICMDAWTNYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTK 180
           NE D + C ICM+AWTNYGEHHICCLPCGHLYGMSCINRWL+QR+ AGKCPQCNRKCS K
Sbjct: 121 NEIDGLFCPICMEAWTNYGEHHICCLPCGHLYGMSCINRWLQQRRKAGKCPQCNRKCSMK 180

Query: 181 DVRKIFAPRIVAADGESEKRIKSLEATCASLEK------KVQVEWKKRETDLQSKLRELT 240
           DVRK+FA RIVAAD ES+KRI+SLEATCASLE       K +VEWKK+ETDLQSK+ ELT
Sbjct: 181 DVRKLFASRIVAADEESQKRIQSLEATCASLETKNANWCKKEVEWKKKETDLQSKVHELT 240

Query: 241 ERTNYLEHLLEDSQRKPVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGA 300
           ERTNYLEHLLED++RK  +A+LDC F+GKSVS+HNFGLRFSGQVSSC FIL+KELLVDGA
Sbjct: 241 ERTNYLEHLLEDTKRKHEVAHLDCIFEGKSVSDHNFGLRFSGQVSSCGFILEKELLVDGA 300

Query: 301 RLVDVDASNHVVLVARRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAAR 360
           R +DVD+SN VVLVARRQSGFGGTHV+TK                      DLHISPAAR
Sbjct: 301 RSIDVDSSNQVVLVARRQSGFGGTHVITK----------------------DLHISPAAR 360

Query: 361 NLVLFSSLGKKLAILSMESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMR 420
            LVL+SSLGKKLA+LSMESN+IILDYDLPDAAWSCSWDQNSSH+VYAGLKNG L++FDMR
Sbjct: 361 KLVLYSSLGKKLAVLSMESNNIILDYDLPDAAWSCSWDQNSSHYVYAGLKNGSLMMFDMR 420

Query: 421 QTVGPLKILQGLTNNPIHTIHSLSSYSTVSND-KSVLSASAYGICQWNFDALEEGPFLIP 480
           QTVGPLK L+G+T+NP+HTIHSLS+  T+SND KSVLSASAYG+CQWNFDA EEGPFL+P
Sbjct: 421 QTVGPLKTLRGITSNPVHTIHSLSNNLTLSNDVKSVLSASAYGVCQWNFDASEEGPFLVP 480

Query: 481 ETNQGVCTSLAYCSSRDELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKE 540
           ETNQGVCTSLAYCSSRDE+VASYRPKVGM +EI YSQ SPSPSHTAGHRVDGCH+AFKKE
Sbjct: 481 ETNQGVCTSLAYCSSRDEIVASYRPKVGMPSEIPYSQISPSPSHTAGHRVDGCHMAFKKE 540

Query: 541 DH--QYSKLSSAYANIDAIRLPKSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHL 600
           ++  ++SKL SA ANIDAIRLPKSTIIDLQDC+SLFV  DAVM +LILQELPSFRS QHL
Sbjct: 541 ENFQKFSKLGSARANIDAIRLPKSTIIDLQDCSSLFVFGDAVMNELILQELPSFRSFQHL 600

Query: 601 KVRKHPVRDVKYSYGFDGGLLSCLSDDILHLFCTKGS 609
           KV KHPVRDVKYSYGF  GLLSCLSDD L L CTKGS
Sbjct: 601 KVHKHPVRDVKYSYGFGRGLLSCLSDDTLQLLCTKGS 612

BLAST of PI0018059 vs. NCBI nr
Match: XP_008462482.1 (PREDICTED: E3 ubiquitin-protein ligase RFWD3 [Cucumis melo])

HSP 1 Score: 1155.6 bits (2988), Expect = 0.0e+00
Identity = 578/620 (93.23%), Postives = 597/620 (96.29%), Query Frame = 0

Query: 1   MPDSDILHVHYEVEDVEDGGEEEEEEE-----DEEETDDSEETVEGHAEQEVDYVPEVLQ 60
           MPDSDILHVHYEVEDV+DGG EEEEEE     D+EETD+SEETVEGHAE+ VDYVPEVLQ
Sbjct: 1   MPDSDILHVHYEVEDVDDGGGEEEEEEEEDDDDDEETDESEETVEGHAEEGVDYVPEVLQ 60

Query: 61  SPRASQTGTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNRNENDDMICSICMDAW 120
           SPRASQTGT+GSAQARVSFWVEEE+DKRRRTEGGEASSSQNEWNRNENDDMICSICMDAW
Sbjct: 61  SPRASQTGTTGSAQARVSFWVEEELDKRRRTEGGEASSSQNEWNRNENDDMICSICMDAW 120

Query: 121 TNYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADG 180
           TNYGEHHICCLPCGHLYGMSCINRWL+QRKNAGKCPQCNRKCSTKDVRK+FAPRIVAADG
Sbjct: 121 TNYGEHHICCLPCGHLYGMSCINRWLQQRKNAGKCPQCNRKCSTKDVRKLFAPRIVAADG 180

Query: 181 ESEKRIKSLEATCASLEKK------VQVEWKKRETDLQSKLRELTERTNYLEHLLEDSQR 240
           ES+KRIKSLEATCASLEKK       +VEWKKRETDLQ KL+EL+ERTNYLEHLLEDSQR
Sbjct: 181 ESQKRIKSLEATCASLEKKDANWCRKEVEWKKRETDLQLKLQELSERTNYLEHLLEDSQR 240

Query: 241 KPVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLVDVDASNHVVLVA 300
           KPVLANLDCTFQGKSVSEHNFGLRFS QVSSCSFILKKEL+VDGARL+DVDASN +VLVA
Sbjct: 241 KPVLANLDCTFQGKSVSEHNFGLRFSSQVSSCSFILKKELMVDGARLIDVDASNQIVLVA 300

Query: 301 RRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAIL 360
           RRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAIL
Sbjct: 301 RRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAIL 360

Query: 361 SMESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMRQTVGPLKILQGLTNN 420
           SMESNHIILDYDLP+AAWSCSWDQNSSHHVYAGLKNGLLV+FDMRQTVGPLKILQGLTNN
Sbjct: 361 SMESNHIILDYDLPEAAWSCSWDQNSSHHVYAGLKNGLLVMFDMRQTVGPLKILQGLTNN 420

Query: 421 PIHTIHSLSSYSTVSNDKSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSR 480
           PIHTIHSLSSYSTVSNDKS+LSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSR
Sbjct: 421 PIHTIHSLSSYSTVSNDKSILSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSR 480

Query: 481 DELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKED-HQYSKLSSAYANID 540
           DELVASYRPKVGMSNEIVYSQPSPSPSHT GHRVDGCHVAFKKED HQ+SKL SAYANID
Sbjct: 481 DELVASYRPKVGMSNEIVYSQPSPSPSHTTGHRVDGCHVAFKKEDHHQFSKLGSAYANID 540

Query: 541 AIRLPKSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHLKVRKHPVRDVKYSYGFD 600
           AIRLPKSTIIDLQ CNSLFVSADAVMGDLILQELPSFRSVQHLKV KHPVRDVKYSYGFD
Sbjct: 541 AIRLPKSTIIDLQGCNSLFVSADAVMGDLILQELPSFRSVQHLKVHKHPVRDVKYSYGFD 600

Query: 601 GGLLSCLSDDILHLFCTKGS 609
            GLLSCLSDDILHLFCTKGS
Sbjct: 601 RGLLSCLSDDILHLFCTKGS 620

BLAST of PI0018059 vs. NCBI nr
Match: XP_004143360.1 (E3 ubiquitin-protein ligase RFWD3 isoform X1 [Cucumis sativus] >XP_011657666.1 E3 ubiquitin-protein ligase RFWD3 isoform X1 [Cucumis sativus] >XP_011657667.1 E3 ubiquitin-protein ligase RFWD3 isoform X1 [Cucumis sativus] >XP_031742978.1 E3 ubiquitin-protein ligase RFWD3 isoform X1 [Cucumis sativus] >XP_031742980.1 E3 ubiquitin-protein ligase RFWD3 isoform X1 [Cucumis sativus] >KGN48190.1 hypothetical protein Csa_002837 [Cucumis sativus])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 580/619 (93.70%), Postives = 598/619 (96.61%), Query Frame = 0

Query: 1   MPDSDILHVHYEVEDVEDGG----EEEEEEEDEEETDDSEETVEGHAEQEVDYVPEVLQS 60
           MPDSDILHVHYEVEDV+DGG    +E+EEEE+EEETDDSEETVEGHAE+EVDYVPEVLQS
Sbjct: 1   MPDSDILHVHYEVEDVDDGGGEEEQEQEEEEEEEETDDSEETVEGHAEEEVDYVPEVLQS 60

Query: 61  PRASQTGTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNRNENDDMICSICMDAWT 120
           PRASQTGT GSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNR+ENDDMICSICMDAWT
Sbjct: 61  PRASQTGTPGSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNRSENDDMICSICMDAWT 120

Query: 121 NYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGE 180
           NYGEHHICCLPCGHLYGMSCINRWL+QRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGE
Sbjct: 121 NYGEHHICCLPCGHLYGMSCINRWLQQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGE 180

Query: 181 SEKRIKSLEATCASLEK------KVQVEWKKRETDLQSKLRELTERTNYLEHLLEDSQRK 240
           S+KRIKSLEATCASLEK      K +VEWKKRETD+QSKLREL+ERTNYLEHLLEDSQRK
Sbjct: 181 SQKRIKSLEATCASLEKKEANWCKKEVEWKKRETDMQSKLRELSERTNYLEHLLEDSQRK 240

Query: 241 PVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLVDVDASNHVVLVAR 300
           PVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARL+DVDASN +VLVAR
Sbjct: 241 PVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLIDVDASNQIVLVAR 300

Query: 301 RQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILS 360
           R  GFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILS
Sbjct: 301 R--GFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILS 360

Query: 361 MESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMRQTVGPLKILQGLTNNP 420
           MESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLV+FDMRQTVGPLKILQGLTNNP
Sbjct: 361 MESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVMFDMRQTVGPLKILQGLTNNP 420

Query: 421 IHTIHSLSSYSTVSNDKSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSRD 480
           IHTIHSLSS+STVSNDKSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSRD
Sbjct: 421 IHTIHSLSSHSTVSNDKSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSRD 480

Query: 481 ELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKED-HQYSKLSSAYANIDA 540
           ELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKED HQ+SKL SA+ANIDA
Sbjct: 481 ELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKEDHHQFSKLGSAHANIDA 540

Query: 541 IRLPKSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHLKVRKHPVRDVKYSYGFDG 600
           IRLPKSTIIDL DCNSLFVSADA+MGDLILQELPSFRSVQHLKV KHPVRDVKYSYGFDG
Sbjct: 541 IRLPKSTIIDLPDCNSLFVSADAIMGDLILQELPSFRSVQHLKVHKHPVRDVKYSYGFDG 600

Query: 601 GLLSCLSDDILHLFCTKGS 609
           GLL CLSDDIL LFCTKGS
Sbjct: 601 GLLGCLSDDILQLFCTKGS 617

BLAST of PI0018059 vs. NCBI nr
Match: XP_038882129.1 (E3 ubiquitin-protein ligase RFWD3 isoform X1 [Benincasa hispida])

HSP 1 Score: 1055.8 bits (2729), Expect = 1.4e-304
Identity = 531/614 (86.48%), Postives = 564/614 (91.86%), Query Frame = 0

Query: 3   DSDILHVHYEVEDVEDGGEEEEEEEDEEETDDSEETVEGHAEQEVDYVPEVLQSPRASQT 62
           D+DI+HVHYEVED ++GG   +EEED+EETDD EETVE HA +E+DYV E++QSPRASQT
Sbjct: 8   DTDIMHVHYEVEDDDNGG---DEEEDDEETDDGEETVEEHAGEEIDYVTEIVQSPRASQT 67

Query: 63  GTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNRNENDDMICSICMDAWTNYGEHH 122
           G S SAQARVSFWVEEEVDKRRRTEGGEA+SSQNE     N+D  CSICM+ WTNYG+HH
Sbjct: 68  GPSESAQARVSFWVEEEVDKRRRTEGGEATSSQNEC----NNDFTCSICMEPWTNYGDHH 127

Query: 123 ICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGESEKRIK 182
           ICCLPCGHLYGM CINRWLRQ KNAGKCPQCNRKCSTKDVRK+FAPRI AADGES+KRI+
Sbjct: 128 ICCLPCGHLYGMYCINRWLRQHKNAGKCPQCNRKCSTKDVRKLFAPRIAAADGESQKRIQ 187

Query: 183 SLEATCASLEK------KVQVEWKKRETDLQSKLRELTERTNYLEHLLEDSQRKPVLANL 242
           SLEATCASLEK      K +VEWKKRETDLQ K+RELTERTNYLEHLLEDSQRKPVLANL
Sbjct: 188 SLEATCASLEKKDADWSKKEVEWKKRETDLQLKVRELTERTNYLEHLLEDSQRKPVLANL 247

Query: 243 DCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLVDVDASNHVVLVARRQSGFG 302
           DCTF GKSVSEHNFGLRFSGQV+SCSFILKKELLVDGARL+DVDASN VVLVARRQSGFG
Sbjct: 248 DCTFHGKSVSEHNFGLRFSGQVASCSFILKKELLVDGARLIDVDASNQVVLVARRQSGFG 307

Query: 303 GTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILSMESNHI 362
           GTHVLTKISLMSPYEREDI IPFCTNAVRDLHISPA RNLVLFSSLGKKLAILSMESNHI
Sbjct: 308 GTHVLTKISLMSPYEREDIRIPFCTNAVRDLHISPAGRNLVLFSSLGKKLAILSMESNHI 367

Query: 363 ILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMRQTVGPLKILQGLTNNPIHTIHS 422
           ILDYDLPDAAWSCSWDQNSSH++YAGLKNG LV+FDMRQTVGPLK LQGLTNNPIHTIHS
Sbjct: 368 ILDYDLPDAAWSCSWDQNSSHYIYAGLKNGSLVMFDMRQTVGPLKTLQGLTNNPIHTIHS 427

Query: 423 LSSYSTVSND-KSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSRDELVAS 482
           LSSYST+SND KSVLSASAYG+CQWNFDA+ EGPFL+PETNQGVCTSLAYC  RDE+VAS
Sbjct: 428 LSSYSTLSNDVKSVLSASAYGVCQWNFDAVGEGPFLVPETNQGVCTSLAYCLGRDEIVAS 487

Query: 483 YRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKEDH--QYSKLSSAYANIDAIRLP 542
           YRPKVGMSNEIVYSQPSPSPSHTAGH V GCHVAFKKED+  Q+SKL SAYA +DAIRLP
Sbjct: 488 YRPKVGMSNEIVYSQPSPSPSHTAGHGVVGCHVAFKKEDNLQQFSKLGSAYAYVDAIRLP 547

Query: 543 KSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHLKVRKHPVRDVKYSYGFDGGLLS 602
           KSTIIDLQDCNSLFVSADAVMGDLILQELPSFRS+QHLKV KHPVRDVKYS G D GLLS
Sbjct: 548 KSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSIQHLKVHKHPVRDVKYSCGLDRGLLS 607

Query: 603 CLSDDILHLFCTKG 608
           CLSDD LHLFCTKG
Sbjct: 608 CLSDDTLHLFCTKG 614

BLAST of PI0018059 vs. NCBI nr
Match: XP_038882130.1 (E3 ubiquitin-protein ligase RFWD3 isoform X2 [Benincasa hispida])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.6e-300
Identity = 526/614 (85.67%), Postives = 558/614 (90.88%), Query Frame = 0

Query: 3   DSDILHVHYEVEDVEDGGEEEEEEEDEEETDDSEETVEGHAEQEVDYVPEVLQSPRASQT 62
           D+DI+HVHYEVED ++GG   +EEED+EETDD EETVE HA +E+DYV E++QSPRASQT
Sbjct: 8   DTDIMHVHYEVEDDDNGG---DEEEDDEETDDGEETVEEHAGEEIDYVTEIVQSPRASQT 67

Query: 63  GTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQNEWNRNENDDMICSICMDAWTNYGEHH 122
           G S SAQARVSFWVEEEVDKRRRTEGGEA+SSQNE     N+D  CSICM+ WTNYG+HH
Sbjct: 68  GPSESAQARVSFWVEEEVDKRRRTEGGEATSSQNEC----NNDFTCSICMEPWTNYGDHH 127

Query: 123 ICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGESEKRIK 182
           ICCLPCGHLYGM CINRWLRQ KNAGKCPQCNRKCSTKDVRK+FAPRI AADGES+KRI+
Sbjct: 128 ICCLPCGHLYGMYCINRWLRQHKNAGKCPQCNRKCSTKDVRKLFAPRIAAADGESQKRIQ 187

Query: 183 SLEATCASLEK------KVQVEWKKRETDLQSKLRELTERTNYLEHLLEDSQRKPVLANL 242
           SLEATCASLEK      K +VEWKKRETDLQ K      RTNYLEHLLEDSQRKPVLANL
Sbjct: 188 SLEATCASLEKKDADWSKKEVEWKKRETDLQLK------RTNYLEHLLEDSQRKPVLANL 247

Query: 243 DCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLVDVDASNHVVLVARRQSGFG 302
           DCTF GKSVSEHNFGLRFSGQV+SCSFILKKELLVDGARL+DVDASN VVLVARRQSGFG
Sbjct: 248 DCTFHGKSVSEHNFGLRFSGQVASCSFILKKELLVDGARLIDVDASNQVVLVARRQSGFG 307

Query: 303 GTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILSMESNHI 362
           GTHVLTKISLMSPYEREDI IPFCTNAVRDLHISPA RNLVLFSSLGKKLAILSMESNHI
Sbjct: 308 GTHVLTKISLMSPYEREDIRIPFCTNAVRDLHISPAGRNLVLFSSLGKKLAILSMESNHI 367

Query: 363 ILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMRQTVGPLKILQGLTNNPIHTIHS 422
           ILDYDLPDAAWSCSWDQNSSH++YAGLKNG LV+FDMRQTVGPLK LQGLTNNPIHTIHS
Sbjct: 368 ILDYDLPDAAWSCSWDQNSSHYIYAGLKNGSLVMFDMRQTVGPLKTLQGLTNNPIHTIHS 427

Query: 423 LSSYSTVSND-KSVLSASAYGICQWNFDALEEGPFLIPETNQGVCTSLAYCSSRDELVAS 482
           LSSYST+SND KSVLSASAYG+CQWNFDA+ EGPFL+PETNQGVCTSLAYC  RDE+VAS
Sbjct: 428 LSSYSTLSNDVKSVLSASAYGVCQWNFDAVGEGPFLVPETNQGVCTSLAYCLGRDEIVAS 487

Query: 483 YRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKEDH--QYSKLSSAYANIDAIRLP 542
           YRPKVGMSNEIVYSQPSPSPSHTAGH V GCHVAFKKED+  Q+SKL SAYA +DAIRLP
Sbjct: 488 YRPKVGMSNEIVYSQPSPSPSHTAGHGVVGCHVAFKKEDNLQQFSKLGSAYAYVDAIRLP 547

Query: 543 KSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHLKVRKHPVRDVKYSYGFDGGLLS 602
           KSTIIDLQDCNSLFVSADAVMGDLILQELPSFRS+QHLKV KHPVRDVKYS G D GLLS
Sbjct: 548 KSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSIQHLKVHKHPVRDVKYSCGLDRGLLS 607

Query: 603 CLSDDILHLFCTKG 608
           CLSDD LHLFCTKG
Sbjct: 608 CLSDDTLHLFCTKG 608

BLAST of PI0018059 vs. NCBI nr
Match: XP_022962837.1 (E3 ubiquitin-protein ligase RFWD3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 974.5 bits (2518), Expect = 4.1e-280
Identity = 502/637 (78.81%), Postives = 551/637 (86.50%), Query Frame = 0

Query: 1   MPDS-----DILHV-----HYEVEDVEDGGEEEEEEEDEEETDDSEETVEGHAEQEVDYV 60
           MPDS     DI +V      YEVED +DG  EEEEE+D+EETDD EETVEGHAE+  D V
Sbjct: 1   MPDSFPSYVDIQNVLVDGEQYEVEDSDDG--EEEEEDDDEETDDGEETVEGHAEENADNV 60

Query: 61  PEVLQSPRASQTGTSGSAQARVSFWVEEEVDKRRRTEGGEASSSQ----------NEWNR 120
            E  QSPRASQTG S S QARVSFWVEEE DKRRRTEGGE SSS           NEWNR
Sbjct: 61  IEA-QSPRASQTGPSRSGQARVSFWVEEEEDKRRRTEGGEGSSSNGVGSVEISQGNEWNR 120

Query: 121 NENDDMICSICMDAWTNYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTK 180
           NE D + C ICM+AWTNYGEHHICCLPCGHLYGMSCINRWL+QR+ AGKCPQCNRKCS K
Sbjct: 121 NEIDGLFCPICMEAWTNYGEHHICCLPCGHLYGMSCINRWLQQRRKAGKCPQCNRKCSMK 180

Query: 181 DVRKIFAPRIVAADGESEKRIKSLEATCASLEK------KVQVEWKKRETDLQSKLRELT 240
           DVRK+FA RIVAAD ES+KRI+SLEATCASLE       K +VEWKK+ETDLQSK+ ELT
Sbjct: 181 DVRKLFASRIVAADEESQKRIQSLEATCASLETKNANWCKKEVEWKKKETDLQSKVHELT 240

Query: 241 ERTNYLEHLLEDSQRKPVLANLDCTFQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGA 300
           ERTNYLEHLLED++RK  +A+LDC F+GKSVS+HNFGLRFSGQVSSC FIL+KELLVDGA
Sbjct: 241 ERTNYLEHLLEDTKRKHEVAHLDCIFEGKSVSDHNFGLRFSGQVSSCGFILEKELLVDGA 300

Query: 301 RLVDVDASNHVVLVARRQSGFGGTHVLTKISLMSPYEREDIGIPFCTNAVRDLHISPAAR 360
           R +DVD+SN VVLVARRQSGFGGTHV+TKISLMSPYEREDI IPFCTNAV+DLHISPAAR
Sbjct: 301 RSIDVDSSNQVVLVARRQSGFGGTHVITKISLMSPYEREDIRIPFCTNAVKDLHISPAAR 360

Query: 361 NLVLFSSLGKKLAILSMESNHIILDYDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMR 420
            LVL+SSLGKKLA+LSMESN+IILDYDLPDAAWSCSWDQNSSH+VYAGLKNG L++FDMR
Sbjct: 361 KLVLYSSLGKKLAVLSMESNNIILDYDLPDAAWSCSWDQNSSHYVYAGLKNGSLMMFDMR 420

Query: 421 QTVGPLKILQGLTNNPIHTIHSLSSYSTVSND-KSVLSASAYGICQWNFDALEEGPFLIP 480
           QTVGPLK L+G+T+NP+HTIHSLS+  T+SND KSVLSASAYG+CQWNFDA EEGPFL+P
Sbjct: 421 QTVGPLKTLRGITSNPVHTIHSLSNNLTLSNDVKSVLSASAYGVCQWNFDASEEGPFLVP 480

Query: 481 ETNQGVCTSLAYCSSRDELVASYRPKVGMSNEIVYSQPSPSPSHTAGHRVDGCHVAFKKE 540
           ETNQGVCTSLAYCSSRDE+VASYRPKVGM +EI YSQ SPSPSHTAGHRVDGCH+AFKKE
Sbjct: 481 ETNQGVCTSLAYCSSRDEIVASYRPKVGMPSEIPYSQISPSPSHTAGHRVDGCHMAFKKE 540

Query: 541 DH--QYSKLSSAYANIDAIRLPKSTIIDLQDCNSLFVSADAVMGDLILQELPSFRSVQHL 600
           ++  ++SKL SA ANIDAIRLPKSTIIDLQDC+SLFV  DAVM +LILQELPSFRS QHL
Sbjct: 541 ENFQKFSKLGSARANIDAIRLPKSTIIDLQDCSSLFVFGDAVMNELILQELPSFRSFQHL 600

Query: 601 KVRKHPVRDVKYSYGFDGGLLSCLSDDILHLFCTKGS 609
           KV KHPVRDVKYSYGF  GLLSCLSDD L L CTKGS
Sbjct: 601 KVHKHPVRDVKYSYGFGRGLLSCLSDDTLQLLCTKGS 634

BLAST of PI0018059 vs. TAIR 10
Match: AT5G03450.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 480.7 bits (1236), Expect = 1.7e-135
Identity = 271/612 (44.28%), Postives = 381/612 (62.25%), Query Frame = 0

Query: 19  GGEEEEEEEDEEET---------DDSEETVEGHAEQEVDYVPEVLQSPRASQTGTSGSAQ 78
           G EE+EEEEDEEE          +D EE  E  +E++     +VL SP            
Sbjct: 30  GEEEDEEEEDEEEAYVDYGEVQEEDEEEEEEEDSERQTREYIDVLDSP------------ 89

Query: 79  ARVSFWVEEEVDKRRRTEGGEASSSQN----EWNRNENDDMICSICMDAWTNYGEHHICC 138
            RVS    E ++ + R++G  +SSS +    EW + + + + CSICM+ WT+ G+H +CC
Sbjct: 90  VRVSQNESEGIENQNRSQGVCSSSSGSQEDVEWKQGDTEGLSCSICMEVWTSGGQHQVCC 149

Query: 139 LPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTKDVRKIFAPRIVAADGESEKRIKSLE 198
           LPCGHLYG SCIN+W +QR++ GKCP CN+ CS +DVRKI+A R+ A D E+ KRI  LE
Sbjct: 150 LPCGHLYGYSCINKWFQQRRSGGKCPLCNKICSLRDVRKIYASRVAAVDDEAHKRILFLE 209

Query: 199 ATCASLEKKV------QVEWKKRETDLQSKLRELTERTNYLEHLLEDSQRKPVLANLDCT 258
           A   S+E+K       +V+WK  E +L+ ++ +L ++   +E   + SQ +  +A+    
Sbjct: 210 AKLRSIEQKTASWSNKEVQWKITEAELRLEVTKLKKKIANMESTTKGSQSQSNVAS---- 269

Query: 259 FQGKSVSEHNFGLRFSGQVSSCSFILKKELLVDGARLVDVDASNHVVLVARRQSGFGGTH 318
            Q K +  HN      GQ  SCSF  + +LL++G R+ D+D    ++L ARR SG GGT 
Sbjct: 270 -QEKYIPAHNIYQEHHGQSPSCSFRHQGDLLINGGRIFDIDGGRQILLYARRLSGVGGTF 329

Query: 319 VLTKISLMSPYEREDIGIPFCTNAVRDLHISPAARNLVLFSSLGKKLAILSMESNHIILD 378
           VLT++SL SP E +DI +P  T A++DLH+SP    L +F SLGKKL+++S+ES++ +L 
Sbjct: 330 VLTQMSLHSPCEIDDILLPRTTRAIKDLHLSPHNNGLAVFGSLGKKLSVISLESHNTVLS 389

Query: 379 YDLPDAAWSCSWDQNSSHHVYAGLKNGLLVIFDMRQTVGPLKILQGLTNNPIHTIHSLSS 438
           YDLP A WSCSWD  SSHH+YAGL+NG+++IFDMRQT GP   + GLT NP+HT+H LS 
Sbjct: 390 YDLPAAPWSCSWDLKSSHHIYAGLQNGMVLIFDMRQTTGPFASMTGLTTNPVHTVHHLSG 449

Query: 439 YSTVSND-KSVLSASAYGICQWNFDALEEGPFLIPET-NQGVCTSLAYCSSRDELVASYR 498
            ST ++D +++LSAS+ G+CQWN    E  P L+ ET N GVC S +YC   + +VASYR
Sbjct: 450 NSTPTSDVRTLLSASSIGLCQWNISGSEGSPSLVSETGNPGVCISSSYCPRSNHIVASYR 509

Query: 499 PKVGMSNEIVYSQPS---PSPSHTAGHRVDGCHVAFKKE--DHQYSKLSSAYANIDAIRL 558
           P+VG S++ V +QPS      S++  + VDG HV  K+   D  Y KLSS  A +D IRL
Sbjct: 510 PRVGSSDDTVPTQPSLTQTGVSNSNSNGVDGFHVCHKRRGVDSYYQKLSSTQAIVDPIRL 569

Query: 559 PKSTIIDL-QDCNSLFVSADAVMGDLILQELPSFRSVQHLKVRKH-PVRDVKYSYGFDGG 603
           P++ IID   +   LF S D    +LILQ+  +F   Q L +  H P++DVKY +     
Sbjct: 570 PRTAIIDFGGERKQLFASCDESTRELILQDPSTFTISQRLPLSSHLPLQDVKYCHVNGSD 624

BLAST of PI0018059 vs. TAIR 10
Match: AT5G03750.1 (BEST Arabidopsis thaliana protein match is: Transducin/WD40 repeat-like superfamily protein (TAIR:AT5G03450.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 65.1 bits (157), Expect = 2.3e-10
Identity = 26/47 (55.32%), Postives = 37/47 (78.72%), Query Frame = 0

Query: 368 SCSWDQNSSHHVYAGLKNGLLVIFDMRQTVGPLKILQGLTNNPIHTI 415
           SCSWD N+ H+VYAGL+NG++++FDMRQ  GPL  L  +T+N +H +
Sbjct: 41  SCSWDLNNYHNVYAGLQNGMVLVFDMRQNRGPLVSLADVTSNSVHIV 87

BLAST of PI0018059 vs. TAIR 10
Match: AT5G24870.1 (RING/U-box superfamily protein )

HSP 1 Score: 44.3 bits (103), Expect = 4.1e-04
Identity = 21/60 (35.00%), Postives = 33/60 (55.00%), Query Frame = 0

Query: 101 NENDDMICSICMDAWTNYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTK 160
           N++DD+ CSIC + + +  E  +  LPC H Y +SC  +WLR +     CP C     ++
Sbjct: 461 NKDDDVKCSICQEEYVDGDE--VGTLPCQHKYHVSCAQQWLRMK---NWCPICKTSAESQ 515

BLAST of PI0018059 vs. TAIR 10
Match: AT5G24870.2 (RING/U-box superfamily protein )

HSP 1 Score: 44.3 bits (103), Expect = 4.1e-04
Identity = 21/60 (35.00%), Postives = 33/60 (55.00%), Query Frame = 0

Query: 101 NENDDMICSICMDAWTNYGEHHICCLPCGHLYGMSCINRWLRQRKNAGKCPQCNRKCSTK 160
           N++DD+ CSIC + + +  E  +  LPC H Y +SC  +WLR +     CP C     ++
Sbjct: 461 NKDDDVKCSICQEEYVDGDE--VGTLPCQHKYHVSCAQQWLRMK---NWCPICKTSAESQ 515