PI0016575 (gene) Melon (PI 482460) v1

Overview
NamePI0016575
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionEf-hand calcium binding protein, putative
Locationchr12: 17364375 .. 17367397 (+)
RNA-Seq ExpressionPI0016575
SyntenyPI0016575
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGGATGAAGAATGTAAAACTAAATAGGTCAAAAATCCCAAACGTCATGGGGGACTAAGGCCCCTTGTATCTAGAATCATCGCTCTCCAATGGCAAATCACATTTCATGGTACCTTCGCGTTTATCATCAATCAAGATGAAAATTTGAAGAAATGGACGCGAAATGAACACCCAAAATCCCAATTTTATCTTCCATTGAAGCAAGCAACCGCGTGTGGTGTAACATAAATTCGTCTTCAAATTCCCTTTTGCTTCCTCTGTTGCATCTCTTTTATTCTTTCTTTTCCATTTTTTTTTCATTTTGAATCCACCTAGAATCTGACCACCGTTTCATCTCAAAAAAAGTTCCCAGATTCCGATACCCTTTTCATTCTTTGCTCTTTCTGATCTTAAATTTATTACCCATTTCTTGTTTCTTTTTGGCCATCAATGGCGTCCTTCTTTAATAGATTCACTTCCTATTCCAATTCCAATTCCAGTTCCAATTCCAATTCCAATTCCCATGCCCCATCTGCACCGTCCGCTCCATCGGCGCCGGAAACACAAGGCCATGAACATTCACATGCGACCGCTTCGCCGTATTACTCCGAACAGCATCAGCCGCAGCCTTATGGGTCCAATTATGGTGGGGTTTCTTCATACGGGTCTTATGGTTTTCCTCCGGGGACCAGCCCTGAGGTAATTAGGAGCTTTCAAATGGTGGATAGAGATGGAAGTGGATTCATTGATGAGAATGAATTGCAGCAAGCCCTTTCTTCTGGCTACCAGAGGTTTAGTCTTAGAACCGTTCGTTTGCTCATCTTCTTATTTAGAAATCCGATTGATTCCTCCAGAATGGGTTAGTTTCTGTTTTGTTCTCTCCTCCAATCTCCTTGGTTTAATGTTGTAATTTAACTTGAGATCGTTCTTTCGACTTCAATCTCTGTTTAATTATACCATCTTTCAAAATAATGAAAATCATGAACATAAACTATTGCCTCATGGTTGAGTTTCTCGGCAAGTTGAATTGGTAACAATTGGTTTGGTTTGACCTATACCAAAGTAAGGCGAATAGAATGTGAAGAAAAGGAAATTGAAAATGTAAATGTGAATACGAATACAGCTTCATTTTATCTTGGATTTCTCAACTGCTTTTAGGATACTTGTATTTTGCTTATTTTTATCATATTCAAAGATTTACTGTATTTGCTGGTGCCTTTTACTTAACTTTGTGTTTCTGAATTGCTTGGTGCAGGGCCTAATGAGTTTACGGCTTTGTGGAACTGTCTTGGCCAATGGCGTGTAAGTAATGTTGCCATTGTGTTGTTACCTTCTTCCTTGATCATTTTGCGCTTATAATCTATCTGTTTTTGCTCTGACTTTTTACAAAAAGATGATGACAATGAAATTAGAACTTCTGACTTAAAGTAGATGATTGGTTTTAAATGTCACTATGAACTGTTGTATGGAAAGATAATTGTTGGTTGTAATGCTGATCTATAACTTGGGTTGGAGTATTTGAATTAAAACCACAATAAGATTTAACAGAAAAGTATTTTAGTTGAACCAATTTGTTGAAGCCATTTGGTGTATGTGTAAAATCACTGAAGTATTTTTGAACTTGTAATATCTTAGTTCAAAGCATGATTTGTTCTACATTTTGTTATTTCATTTAGAAAGAAAGACTCTCTTGCTTCTATAGCTAATTAGTAGCACATGCGCCATGTATGCTTCTCTTCATTTAAAGTCAATGTTACTATTTAATTTTTAAGTTTTTCCAAATTCTAAGGATCTTTTTAGGATAATATTTTTTAGATTCTTGAGGAAATAGGGTAGATAAGAAGAAAATAAAACAAGAGAGCAACCTATTAATCTAATTATTTGGATTTAATTGGCAATTTCTTAATAGAGCATCCTTATAAGGACATTTCCAGATTAAAATTTTCTTTTTTTCTTTTTTGAGATTTTTTGAAGGATAATCAACTTTATGGCGTATGAAACTTAGAATGAATTTTACTAATTTTCAGGGCATGTTCGAGAGATATGATAGAGATAGAAGTGGGAGAATTGATGCACTGGAAATGAGGGATGCACTCTATGGTCTTGGCTATGCAGTTCCATCCTCAGTCCTTCAACTTCTAATTTCACTATACGATGATCGAAGTGGTCAACAAGTGGAATTTAATTTTGACAGTTTTGTCGAGTAAGTGTTTCTAAGTAGTAGAATTCTTATATAGCTTTTTATATGCCAAAAACCTTGGATACTTACATTTCTGGATTTGTCTATTTTCTTTTTGCAGATGTGGCATGATAGTTAAGGTAAGATTATAGACCTTTCACTCCTTATTTTCGAAAGAAAAAGATTCCACTAGTATTATAACGTCTACTCGGCTATTTAATACTGTAGATAGATCTTGCCAACGTAATTGAATGTGGCTTTCTATGTTTAAACCAATGCCACGGAGTTCATCTCTTTAAGTAAAAACCACAAGTGCCTATTCATAGCTATTAGAAGTTTTAGTATAATCCTCTTATTAACAGCACAAAATAATTTTGAGTGTGATATCATTTCTAAACCAATGCTTGCAAAATTTCAGGGATTGACTGAGAAATTCAAGGAGAAGGATAGGCACTACACGGGTTCAGCGACTCTTACGTATGAAGATTTCATGTCAACAATCCTCCCATTTCTTGTGTCATACAGTTAAAACCCTCAAAGGTGATCGATATCTCTTCACCTCCTGGATTTTGTGTAATATCATCTCTTCATTTGTTGTTTACTTGAAAATGCTAGAAGCTGTACATCCAAGGGAAGCTGCTGCTTTAGACCAAAAGGTTTATATTGGCATGAGAGAGTTTCCATGTACAAAGATTTATTTGTTATTTTGTTTCTTCTGTTTTGATTCTGCAAGATAGAATTCTTCGTTTGTTGGTTTTTTAGATTGTTTGCGACCGATTTTAATCAAGTTTTGGAACTTGTACTAGAAAGTGAAGGAATAAACCGGCCATGCCTTATTATGAGGGAGTTCATGTTTTGTTC

mRNA sequence

AAAAAAGGATGAAGAATGTAAAACTAAATAGGTCAAAAATCCCAAACGTCATGGGGGACTAAGGCCCCTTGTATCTAGAATCATCGCTCTCCAATGGCAAATCACATTTCATGGTACCTTCGCGTTTATCATCAATCAAGATGAAAATTTGAAGAAATGGACGCGAAATGAACACCCAAAATCCCAATTTTATCTTCCATTGAAGCAAGCAACCGCGTGTGGTGTAACATAAATTCGTCTTCAAATTCCCTTTTGCTTCCTCTGTTGCATCTCTTTTATTCTTTCTTTTCCATTTTTTTTTCATTTTGAATCCACCTAGAATCTGACCACCGTTTCATCTCAAAAAAAGTTCCCAGATTCCGATACCCTTTTCATTCTTTGCTCTTTCTGATCTTAAATTTATTACCCATTTCTTGTTTCTTTTTGGCCATCAATGGCGTCCTTCTTTAATAGATTCACTTCCTATTCCAATTCCAATTCCAGTTCCAATTCCAATTCCAATTCCCATGCCCCATCTGCACCGTCCGCTCCATCGGCGCCGGAAACACAAGGCCATGAACATTCACATGCGACCGCTTCGCCGTATTACTCCGAACAGCATCAGCCGCAGCCTTATGGGTCCAATTATGGTGGGGTTTCTTCATACGGGTCTTATGGTTTTCCTCCGGGGACCAGCCCTGAGGTAATTAGGAGCTTTCAAATGGTGGATAGAGATGGAAGTGGATTCATTGATGAGAATGAATTGCAGCAAGCCCTTTCTTCTGGCTACCAGAGGTTTAGTCTTAGAACCGTTCGTTTGCTCATCTTCTTATTTAGAAATCCGATTGATTCCTCCAGAATGGGGCCTAATGAGTTTACGGCTTTGTGGAACTGTCTTGGCCAATGGCGTGGCATGTTCGAGAGATATGATAGAGATAGAAGTGGGAGAATTGATGCACTGGAAATGAGGGATGCACTCTATGGTCTTGGCTATGCAGTTCCATCCTCAGTCCTTCAACTTCTAATTTCACTATACGATGATCGAAGTGGTCAACAAGTGGAATTTAATTTTGACAGTTTTGTCGAATGTGGCATGATAGTTAAGGGATTGACTGAGAAATTCAAGGAGAAGGATAGGCACTACACGGGTTCAGCGACTCTTACGTATGAAGATTTCATGTCAACAATCCTCCCATTTCTTGTGTCATACAGTTAAAACCCTCAAAGGTGATCGATATCTCTTCACCTCCTGGATTTTGTGTAATATCATCTCTTCATTTGTTGTTTACTTGAAAATGCTAGAAGCTGTACATCCAAGGGAAGCTGCTGCTTTAGACCAAAAGGTTTATATTGGCATGAGAGAGTTTCCATGTACAAAGATTTATTTGTTATTTTGTTTCTTCTGTTTTGATTCTGCAAGATAGAATTCTTCGTTTGTTGGTTTTTTAGATTGTTTGCGACCGATTTTAATCAAGTTTTGGAACTTGTACTAGAAAGTGAAGGAATAAACCGGCCATGCCTTATTATGAGGGAGTTCATGTTTTGTTC

Coding sequence (CDS)

ATGGCGTCCTTCTTTAATAGATTCACTTCCTATTCCAATTCCAATTCCAGTTCCAATTCCAATTCCAATTCCCATGCCCCATCTGCACCGTCCGCTCCATCGGCGCCGGAAACACAAGGCCATGAACATTCACATGCGACCGCTTCGCCGTATTACTCCGAACAGCATCAGCCGCAGCCTTATGGGTCCAATTATGGTGGGGTTTCTTCATACGGGTCTTATGGTTTTCCTCCGGGGACCAGCCCTGAGGTAATTAGGAGCTTTCAAATGGTGGATAGAGATGGAAGTGGATTCATTGATGAGAATGAATTGCAGCAAGCCCTTTCTTCTGGCTACCAGAGGTTTAGTCTTAGAACCGTTCGTTTGCTCATCTTCTTATTTAGAAATCCGATTGATTCCTCCAGAATGGGGCCTAATGAGTTTACGGCTTTGTGGAACTGTCTTGGCCAATGGCGTGGCATGTTCGAGAGATATGATAGAGATAGAAGTGGGAGAATTGATGCACTGGAAATGAGGGATGCACTCTATGGTCTTGGCTATGCAGTTCCATCCTCAGTCCTTCAACTTCTAATTTCACTATACGATGATCGAAGTGGTCAACAAGTGGAATTTAATTTTGACAGTTTTGTCGAATGTGGCATGATAGTTAAGGGATTGACTGAGAAATTCAAGGAGAAGGATAGGCACTACACGGGTTCAGCGACTCTTACGTATGAAGATTTCATGTCAACAATCCTCCCATTTCTTGTGTCATACAGTTAA

Protein sequence

MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMSTILPFLVSYS
Homology
BLAST of PI0016575 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 5.3e-59
Identity = 119/235 (50.64%), Postives = 165/235 (70.21%), Query Frame = 0

Query: 20  SNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQPYGSNYGGVSSYGSYGFPPG 79
           S SN++APSAP  P +   Q H+        Y S  +QP    S+Y G+       F P 
Sbjct: 2   SYSNAYAPSAPELPESFVQQQHDGESRYTYAYPS--YQPTQQFSSYSGM-------FSPE 61

Query: 80  TSPEVIRSFQMVDRDGSGFIDENELQQALS-SGYQRFSLRTVRLLIFLFRNPIDS-SRMG 139
           T PE++RSF+  DR+ SGF++E+EL+QALS SGY   S RT+RLL+F+++ P+DS  R+G
Sbjct: 62  THPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLG 121

Query: 140 PNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSVLQLLISLYDDR 199
           P E+  LWNCL QWR +F RYDRDRSG++++ ++RDA Y LG  +P+SV QL++S +DD 
Sbjct: 122 PKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDG 181

Query: 200 SGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMSTILPFLVSY 253
           +G+ V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ FM  ++PF+ +Y
Sbjct: 182 TGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFIATY 227

BLAST of PI0016575 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 4.8e-52
Identity = 116/245 (47.35%), Postives = 153/245 (62.45%), Query Frame = 0

Query: 14  SNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQPYGSNYGGVSSYGS 73
           S+SS+          AP +     + G   +  ++ PY +    P P  + YG    YGS
Sbjct: 49  SSSSAPYYGGGGGYGAPPSTQPYGSGGGYGAPPSSQPYGAPYGAPPPSSAPYGAPGGYGS 108

Query: 74  -------YGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVRLLIFL 133
                    FPPGT P V+  FQ  DRDGSG ID+ ELQ ALS   Q FSLRTV LL++L
Sbjct: 109 PFASLVPSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVHLLMYL 168

Query: 134 FRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSV 193
           F N  +  ++GP EFT+++  L  WR +FER+DRDRSG+IDA E+RDAL  LGY+V  +V
Sbjct: 169 FTN-TNVRKIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATELRDALLSLGYSVSPTV 228

Query: 194 LQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMSTIL 252
           L LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD  ++GSAT TYE FM T+L
Sbjct: 229 LDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFSGSATFTYEAFMLTVL 288

BLAST of PI0016575 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.0e-50
Identity = 118/261 (45.21%), Postives = 157/261 (60.15%), Query Frame = 0

Query: 11  YSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQH------QPQP---- 70
           Y     +   + +S   S  + P   +  GH   +    P+    +       P+P    
Sbjct: 95  YGGGYGAPPPSGSSDYGSYGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSG 154

Query: 71  YGSNYGGV---SSYGS-------YGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSS 130
           +G  YGG    +SYGS        GF PGT P ++  FQ  D+DGSGFID+ ELQ ALSS
Sbjct: 155 HGGGYGGYPPQASYGSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSS 214

Query: 131 GYQRFSLRTVRLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALE 190
             QRFS+RTV LL++LF N  ++ ++GP EFTAL+  L  WR +FER D+DRSGRID  E
Sbjct: 215 YQQRFSMRTVHLLMYLFTNS-NAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNE 274

Query: 191 MRDALYGLGYAVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHY 250
           +RDAL  LG++V   VL LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD  Y
Sbjct: 275 LRDALLSLGFSVSPVVLDLLVSKFDKSGGKNRAIEYDNFIECCLTVKGLTEKFKEKDTAY 334

Query: 251 TGSATLTYEDFMSTILPFLVS 252
           +GSAT  YE FM T+LPFL++
Sbjct: 335 SGSATFNYESFMLTVLPFLIA 354

BLAST of PI0016575 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.3e-49
Identity = 115/250 (46.00%), Postives = 148/250 (59.20%), Query Frame = 0

Query: 16  SSSNSNSNSHAPSAPS----APSAPETQGHEHSHATASPYYSEQHQPQPYGSNYGGVSSY 75
           S S     +H  S PS       AP+  GH   +  A        Q   +G  YG     
Sbjct: 94  SPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGAPPPQ 153

Query: 76  GSYG----------FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVR 135
            SYG          FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+RTV 
Sbjct: 154 ASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVH 213

Query: 136 LLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYA 195
           LL++LF N  +  ++GP EFT+L+  L  WR +FER+D+DRSGRID  E+RDAL  LG++
Sbjct: 214 LLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFS 273

Query: 196 VPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDF 252
           V   +L LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD   +GSA   YE+F
Sbjct: 274 VSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKFKEKDTALSGSAIFNYENF 333

BLAST of PI0016575 vs. ExPASy Swiss-Prot
Match: Q5PQ53 (Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 8.8e-30
Identity = 80/221 (36.20%), Postives = 123/221 (55.66%), Query Frame = 0

Query: 24  SHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQPYGSNYGGVSSYGSYGFPPGTSPE 83
           S AP AP  P   +  G  +S   ++PY S+QH     G+  G +        PPG  PE
Sbjct: 66  SAAPGAPGGPYGGQAPGGPYSVPGSTPYGSQQHGSYGQGAPAGNI--------PPGVDPE 125

Query: 84  VIRSFQMVDRDGSGFIDENELQQAL-SSGYQRFSLRTVRLLIFLFRNPIDSSRMGPNEFT 143
               FQ VD D SG+I   EL+QAL ++ +  F+  T  +++ +F +  +S R+    F+
Sbjct: 126 AFSWFQTVDTDHSGYISLKELKQALVNTNWSSFNDETCTMMMNMF-DKSNSGRIDMFGFS 185

Query: 144 ALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSVLQLLISLYDDRSGQQV 203
           ALW  + QWR +F++YDRDRSG I+  E+  AL  +GY +    +Q+++S Y  RS Q  
Sbjct: 186 ALWRFIQQWRNLFQQYDRDRSGSINQGELHQALCQMGYQLSPQFVQIVMSRYAQRSAQP- 245

Query: 204 EFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMS 244
               D F++    ++ +TE F+EKD    G+A L+YEDF++
Sbjct: 246 GLQLDRFIQICTQLQSMTEAFREKDTGQIGTAKLSYEDFIT 276

BLAST of PI0016575 vs. ExPASy TrEMBL
Match: A0A5D3E617 (Putative calcium-binding protein CML48 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003180 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 2.7e-135
Identity = 248/253 (98.02%), Postives = 252/253 (99.60%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQP 60
           MASFFNRFTSYSNS+S+SNSNSNSHAPSAPSAPSAPETQGHE SHATASPYYSEQHQPQP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNSHAPSAPSAPSAPETQGHELSHATASPYYSEQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 254
           FMSTILPFLVSY+
Sbjct: 241 FMSTILPFLVSYN 253

BLAST of PI0016575 vs. ExPASy TrEMBL
Match: A0A1S3CFY8 (probable calcium-binding protein CML48 OS=Cucumis melo OX=3656 GN=LOC103500001 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 2.7e-135
Identity = 248/253 (98.02%), Postives = 252/253 (99.60%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQP 60
           MASFFNRFTSYSNS+S+SNSNSNSHAPSAPSAPSAPETQGHE SHATASPYYSEQHQPQP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNSHAPSAPSAPSAPETQGHELSHATASPYYSEQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 254
           FMSTILPFLVSY+
Sbjct: 241 FMSTILPFLVSYN 253

BLAST of PI0016575 vs. ExPASy TrEMBL
Match: A0A0A0LT26 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G164670 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.4e-131
Identity = 244/253 (96.44%), Postives = 247/253 (97.63%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQP 60
           MASFFNRFTSY  SNSSSNSNSNSHAPSAPS PSAPETQG EHSHATASPY+SEQ+QPQP
Sbjct: 1   MASFFNRFTSY--SNSSSNSNSNSHAPSAPSPPSAPETQGQEHSHATASPYHSEQNQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YG NYGGVSSYGSYGFPPGTSPEVIRSFQMVDRD SGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGFNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDRSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 254
           FMSTILPFLVSYS
Sbjct: 241 FMSTILPFLVSYS 251

BLAST of PI0016575 vs. ExPASy TrEMBL
Match: A0A6J1HR40 (probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC111466605 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.2e-126
Identity = 232/253 (91.70%), Postives = 242/253 (95.65%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQP 60
           MASFF RFTSYSN    SNS+S+SHAPSAPSAPS PET GH +SH TASPYYS+QHQPQP
Sbjct: 1   MASFFGRFTSYSN----SNSHSHSHAPSAPSAPSEPETYGHGNSHGTASPYYSQQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVD+D SGFIDENELQ+ALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRG FERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSG++VE+NFDSFVECGMIVKGLTEKFKEKD+HYTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDKHYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 254
           FMSTILPFLVSYS
Sbjct: 241 FMSTILPFLVSYS 249

BLAST of PI0016575 vs. ExPASy TrEMBL
Match: A0A6J1EVZ6 (probable calcium-binding protein CML48 OS=Cucurbita moschata OX=3662 GN=LOC111438517 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.2e-124
Identity = 229/253 (90.51%), Postives = 237/253 (93.68%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQP 60
           MASFF RFTS+    SSS+SNS  HAPSAP APSAPE  GHEHSH TASPY+SE H PQP
Sbjct: 1   MASFFGRFTSH----SSSSSNSKPHAPSAPYAPSAPEVHGHEHSHGTASPYFSEHHPPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYG VSSYGSYGFPPGT PEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGAVSSYGSYGFPPGTPPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNP+DSSRMGPNEFTALWNCLGQWRG FERYDRDRSG+IDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPVDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGKIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVL+LLISLYDDRSG++VEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED
Sbjct: 181 AVPSSVLELLISLYDDRSGRRVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 254
           FMSTILPFLVSYS
Sbjct: 241 FMSTILPFLVSYS 249

BLAST of PI0016575 vs. NCBI nr
Match: XP_008461406.1 (PREDICTED: probable calcium-binding protein CML48 [Cucumis melo] >KAA0035465.1 putative calcium-binding protein CML48 [Cucumis melo var. makuwa] >TYK31060.1 putative calcium-binding protein CML48 [Cucumis melo var. makuwa])

HSP 1 Score: 491.1 bits (1263), Expect = 5.7e-135
Identity = 248/253 (98.02%), Postives = 252/253 (99.60%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQP 60
           MASFFNRFTSYSNS+S+SNSNSNSHAPSAPSAPSAPETQGHE SHATASPYYSEQHQPQP
Sbjct: 1   MASFFNRFTSYSNSSSNSNSNSNSHAPSAPSAPSAPETQGHELSHATASPYYSEQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 254
           FMSTILPFLVSY+
Sbjct: 241 FMSTILPFLVSYN 253

BLAST of PI0016575 vs. NCBI nr
Match: XP_038895549.1 (probable calcium-binding protein CML48 [Benincasa hispida])

HSP 1 Score: 486.1 bits (1250), Expect = 1.8e-133
Identity = 244/253 (96.44%), Postives = 249/253 (98.42%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQP 60
           MASFFNRFTSYSNSN  SNSNSNSHAPSAPS PSAPETQGHEHSHATASPYYSEQH+PQP
Sbjct: 1   MASFFNRFTSYSNSN--SNSNSNSHAPSAPSVPSAPETQGHEHSHATASPYYSEQHRPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQ+FSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQKFSLRTV 120

Query: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNP+DSSRMGPNEF ALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPVDSSRMGPNEFAALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSG++VEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGERVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 254
           FMSTILPFLVSYS
Sbjct: 241 FMSTILPFLVSYS 251

BLAST of PI0016575 vs. NCBI nr
Match: XP_004139489.1 (probable calcium-binding protein CML48 [Cucumis sativus] >KGN64933.1 hypothetical protein Csa_022705 [Cucumis sativus])

HSP 1 Score: 476.9 bits (1226), Expect = 1.1e-130
Identity = 244/253 (96.44%), Postives = 247/253 (97.63%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQP 60
           MASFFNRFTSY  SNSSSNSNSNSHAPSAPS PSAPETQG EHSHATASPY+SEQ+QPQP
Sbjct: 1   MASFFNRFTSY--SNSSSNSNSNSHAPSAPSPPSAPETQGQEHSHATASPYHSEQNQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YG NYGGVSSYGSYGFPPGTSPEVIRSFQMVDRD SGFIDENELQQALSSGYQRFSLRTV
Sbjct: 61  YGFNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDRSGFIDENELQQALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDR+YTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRNYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 254
           FMSTILPFLVSYS
Sbjct: 241 FMSTILPFLVSYS 251

BLAST of PI0016575 vs. NCBI nr
Match: XP_022967101.1 (probable calcium-binding protein CML48 [Cucurbita maxima])

HSP 1 Score: 460.3 bits (1183), Expect = 1.1e-125
Identity = 232/253 (91.70%), Postives = 242/253 (95.65%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQP 60
           MASFF RFTSYSN    SNS+S+SHAPSAPSAPS PET GH +SH TASPYYS+QHQPQP
Sbjct: 1   MASFFGRFTSYSN----SNSHSHSHAPSAPSAPSEPETYGHGNSHGTASPYYSQQHQPQP 60

Query: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTV 120
           YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVD+D SGFIDENELQ+ALSSGYQRFSLRTV
Sbjct: 61  YGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLRTV 120

Query: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGY 180
           RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRG FERYDRDRSGRIDALEMRDALYGLGY
Sbjct: 121 RLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGRIDALEMRDALYGLGY 180

Query: 181 AVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYED 240
           AVPSSVLQLLISLYDDRSG++VE+NFDSFVECGMIVKGLTEKFKEKD+HYTGSATLTYED
Sbjct: 181 AVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDKHYTGSATLTYED 240

Query: 241 FMSTILPFLVSYS 254
           FMSTILPFLVSYS
Sbjct: 241 FMSTILPFLVSYS 249

BLAST of PI0016575 vs. NCBI nr
Match: XP_023541181.1 (probable calcium-binding protein CML48 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.0 bits (1177), Expect = 5.3e-125
Identity = 231/255 (90.59%), Postives = 245/255 (96.08%), Query Frame = 0

Query: 1   MASFFNRFTSYSNSN--SSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQP 60
           MASFF RFTS+SNSN  S S+S+S+SHAPSAPSAPS PET GH +SH T+SPYYS+QHQP
Sbjct: 1   MASFFGRFTSHSNSNSHSHSHSHSHSHAPSAPSAPSEPETYGHGNSHGTSSPYYSQQHQP 60

Query: 61  QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLR 120
           QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVD+D SGFIDENELQ+ALSSGYQRFSLR
Sbjct: 61  QPYGSNYGGVSSYGSYGFPPGTSPEVIRSFQMVDKDRSGFIDENELQEALSSGYQRFSLR 120

Query: 121 TVRLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGL 180
           TVRLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRG FERYDRDRSGRIDA+EMRDALYGL
Sbjct: 121 TVRLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGTFERYDRDRSGRIDAMEMRDALYGL 180

Query: 181 GYAVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTY 240
           GYAVPSSVLQLLISLYDDRSG++VE+NFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTY
Sbjct: 181 GYAVPSSVLQLLISLYDDRSGRRVEYNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTY 240

Query: 241 EDFMSTILPFLVSYS 254
           EDFM+TILPFLVSYS
Sbjct: 241 EDFMATILPFLVSYS 255

BLAST of PI0016575 vs. TAIR 10
Match: AT2G27480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 229.2 bits (583), Expect = 3.8e-60
Identity = 119/235 (50.64%), Postives = 165/235 (70.21%), Query Frame = 0

Query: 20  SNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQHQPQPYGSNYGGVSSYGSYGFPPG 79
           S SN++APSAP  P +   Q H+        Y S  +QP    S+Y G+       F P 
Sbjct: 2   SYSNAYAPSAPELPESFVQQQHDGESRYTYAYPS--YQPTQQFSSYSGM-------FSPE 61

Query: 80  TSPEVIRSFQMVDRDGSGFIDENELQQALS-SGYQRFSLRTVRLLIFLFRNPIDS-SRMG 139
           T PE++RSF+  DR+ SGF++E+EL+QALS SGY   S RT+RLL+F+++ P+DS  R+G
Sbjct: 62  THPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLG 121

Query: 140 PNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYAVPSSVLQLLISLYDDR 199
           P E+  LWNCL QWR +F RYDRDRSG++++ ++RDA Y LG  +P+SV QL++S +DD 
Sbjct: 122 PKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDG 181

Query: 200 SGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDFMSTILPFLVSY 253
           +G+ V+  FDSF+ECGMIVKGLTEKF+E D  YTG ATL+Y+ FM  ++PF+ +Y
Sbjct: 182 TGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFIATY 227

BLAST of PI0016575 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 200.7 bits (509), Expect = 1.4e-51
Identity = 118/261 (45.21%), Postives = 157/261 (60.15%), Query Frame = 0

Query: 11  YSNSNSSSNSNSNSHAPSAPSAPSAPETQGHEHSHATASPYYSEQH------QPQP---- 70
           Y     +   + +S   S  + P   +  GH   +    P+    +       P+P    
Sbjct: 95  YGGGYGAPPPSGSSDYGSYGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSG 154

Query: 71  YGSNYGGV---SSYGS-------YGFPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSS 130
           +G  YGG    +SYGS        GF PGT P ++  FQ  D+DGSGFID+ ELQ ALSS
Sbjct: 155 HGGGYGGYPPQASYGSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSS 214

Query: 131 GYQRFSLRTVRLLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALE 190
             QRFS+RTV LL++LF N  ++ ++GP EFTAL+  L  WR +FER D+DRSGRID  E
Sbjct: 215 YQQRFSMRTVHLLMYLFTNS-NAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNE 274

Query: 191 MRDALYGLGYAVPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHY 250
           +RDAL  LG++V   VL LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD  Y
Sbjct: 275 LRDALLSLGFSVSPVVLDLLVSKFDKSGGKNRAIEYDNFIECCLTVKGLTEKFKEKDTAY 334

Query: 251 TGSATLTYEDFMSTILPFLVS 252
           +GSAT  YE FM T+LPFL++
Sbjct: 335 SGSATFNYESFMLTVLPFLIA 354

BLAST of PI0016575 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 198.0 bits (502), Expect = 9.3e-51
Identity = 115/250 (46.00%), Postives = 148/250 (59.20%), Query Frame = 0

Query: 16  SSSNSNSNSHAPSAPS----APSAPETQGHEHSHATASPYYSEQHQPQPYGSNYGGVSSY 75
           S S     +H  S PS       AP+  GH   +  A        Q   +G  YG     
Sbjct: 94  SPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGAPPPQ 153

Query: 76  GSYG----------FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVR 135
            SYG          FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+RTV 
Sbjct: 154 ASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVH 213

Query: 136 LLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYA 195
           LL++LF N  +  ++GP EFT+L+  L  WR +FER+D+DRSGRID  E+RDAL  LG++
Sbjct: 214 LLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFS 273

Query: 196 VPSSVLQLLISLYDDRSGQQVEFNFDSFVECGMIVKGLTEKFKEKDRHYTGSATLTYEDF 252
           V   +L LL+S +D   G+     +D+F+EC + VKGLTEKFKEKD   +GSA   YE+F
Sbjct: 274 VSPVILDLLVSKFDKSGGRNRAIEYDNFIECCLTVKGLTEKFKEKDTALSGSAIFNYENF 333

BLAST of PI0016575 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 146.0 bits (367), Expect = 4.2e-35
Identity = 89/210 (42.38%), Postives = 117/210 (55.71%), Query Frame = 0

Query: 16  SSSNSNSNSHAPSAPS----APSAPETQGHEHSHATASPYYSEQHQPQPYGSNYGGVSSY 75
           S S     +H  S PS       AP+  GH   +  A        Q   +G  YG     
Sbjct: 94  SPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGAPPPQ 153

Query: 76  GSYG----------FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVR 135
            SYG          FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+RTV 
Sbjct: 154 ASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVH 213

Query: 136 LLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYA 195
           LL++LF N  +  ++GP EFT+L+  L  WR +FER+D+DRSGRID  E+RDAL  LG++
Sbjct: 214 LLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFS 273

Query: 196 VPSSVLQLLISLYDDRSGQQVEFNFDSFVE 212
           V   +L LL+S +D   G+     +D+F+E
Sbjct: 274 VSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

BLAST of PI0016575 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 146.0 bits (367), Expect = 4.2e-35
Identity = 89/210 (42.38%), Postives = 117/210 (55.71%), Query Frame = 0

Query: 16  SSSNSNSNSHAPSAPS----APSAPETQGHEHSHATASPYYSEQHQPQPYGSNYGGVSSY 75
           S S     +H  S PS       AP+  GH   +  A        Q   +G  YG     
Sbjct: 94  SPSPGGYGAHPSSGPSDYGGYGGAPQQSGHGGGYGGAP-------QQSGHGGGYGAPPPQ 153

Query: 76  GSYG----------FPPGTSPEVIRSFQMVDRDGSGFIDENELQQALSSGYQRFSLRTVR 135
            SYG          FPPGT P ++  FQ  DRD SGFID+ ELQ ALSS  Q FS+RTV 
Sbjct: 154 ASYGSPFASLVPSAFPPGTDPNIVACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVH 213

Query: 136 LLIFLFRNPIDSSRMGPNEFTALWNCLGQWRGMFERYDRDRSGRIDALEMRDALYGLGYA 195
           LL++LF N  +  ++GP EFT+L+  L  WR +FER+D+DRSGRID  E+RDAL  LG++
Sbjct: 214 LLMYLFTNS-NVRKIGPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFS 273

Query: 196 VPSSVLQLLISLYDDRSGQQVEFNFDSFVE 212
           V   +L LL+S +D   G+     +D+F+E
Sbjct: 274 VSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZQH15.3e-5950.64Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ... [more]
Q2QY104.8e-5247.35Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2... [more]
Q9FYE42.0e-5045.21Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ... [more]
Q8W4L01.3e-4946.00Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ... [more]
Q5PQ538.8e-3036.20Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3E6172.7e-13598.02Putative calcium-binding protein CML48 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CFY82.7e-13598.02probable calcium-binding protein CML48 OS=Cucumis melo OX=3656 GN=LOC103500001 P... [more]
A0A0A0LT265.4e-13196.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G164670 PE=4 SV=1[more]
A0A6J1HR405.2e-12691.70probable calcium-binding protein CML48 OS=Cucurbita maxima OX=3661 GN=LOC1114666... [more]
A0A6J1EVZ62.2e-12490.51probable calcium-binding protein CML48 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
Match NameE-valueIdentityDescription
XP_008461406.15.7e-13598.02PREDICTED: probable calcium-binding protein CML48 [Cucumis melo] >KAA0035465.1 p... [more]
XP_038895549.11.8e-13396.44probable calcium-binding protein CML48 [Benincasa hispida][more]
XP_004139489.11.1e-13096.44probable calcium-binding protein CML48 [Cucumis sativus] >KGN64933.1 hypothetica... [more]
XP_022967101.11.1e-12591.70probable calcium-binding protein CML48 [Cucurbita maxima][more]
XP_023541181.15.3e-12590.59probable calcium-binding protein CML48 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G27480.13.8e-6050.64Calcium-binding EF-hand family protein [more]
AT5G04170.11.4e-5145.21Calcium-binding EF-hand family protein [more]
AT3G10300.39.3e-5146.00Calcium-binding EF-hand family protein [more]
AT3G10300.24.2e-3542.38Calcium-binding EF-hand family protein [more]
AT3G10300.44.2e-3542.38Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 83..111
e-value: 1.9E-4
score: 30.8
coord: 150..178
e-value: 0.032
score: 23.4
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 154..172
e-value: 0.014
score: 14.9
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 84..110
e-value: 1.9E-6
score: 27.0
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 146..181
score: 12.337564
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 79..114
score: 12.198088
NoneNo IPR availableGENE3D1.10.238.10coord: 73..250
e-value: 1.1E-36
score: 128.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..64
NoneNo IPR availablePANTHERPTHR46824:SF2CALCIUM-BINDING PROTEIN CML48-RELATEDcoord: 19..251
NoneNo IPR availableCDDcd16180EFh_PEF_Group_Icoord: 83..248
e-value: 3.66833E-70
score: 210.848
IPR044590Probable calcium-binding protein CML48/49/50PANTHERPTHR46824CALCIUM-BINDING PROTEIN CML48-RELATEDcoord: 19..251
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 92..104
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 159..171
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 75..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0016575.1PI0016575.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding