PI0016300 (gene) Melon (PI 482460) v1

BLAST of PI0016300 vs. ExPASy Swiss-Prot
Match: Q9FRK5 (Uncharacterized membrane protein At1g75140 OS=Arabidopsis thaliana OX=3702 GN=At1g75140 PE=1 SV=3)

HSP 1 Score: 644.0 bits (1660), Expect = 1.8e-183
Identity = 352/650 (54.15%), Postives = 453/650 (69.69%), Query Frame = 0

Query: 9   FFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSE-----IEVPFEIKQYDLLKNLEELVRNL 68
           FFF+  +L+F  P  S V    S       S+     +      +Q   L  LEELVRNL
Sbjct: 13  FFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGVSLHRLEELVRNL 72

Query: 69  SDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLDNKIQ 128
           +++V++L+ +LS+ P  V +E         +  +  +EK K                   
Sbjct: 73  TELVARLDAKLSETPFKVKKE---------ITRDEIEEKAK------------------- 132

Query: 129 DGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDERGRI 188
                 A SVTKYS FWSERF F SAVKL+S+ATCINVLP RD EG SKY AVGD  GR+
Sbjct: 133 ------AFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGLSKYFAVGDSSGRV 192

Query: 189 YVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSNGEDL 248
           +VF+RNGDV +E  T  +SPITAM+SYMS+YKNE+ +VTGH++GVIL+HR+ EGS GEDL
Sbjct: 193 FVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVILLHRLREGSIGEDL 252

Query: 249 NLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTVYGSVM 308
           N   ME+V +F   D  ED  Q++LLE+HHVGR RYIL++D  GK+ VF E+ TVYGSV 
Sbjct: 253 NSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLTVFTENRTVYGSVS 312

Query: 309 PTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSKAYGF 368
           PTSRP+ FLKQRLLFLTE+GAGSLDLRSMK+RESECEGLNHSLAR+YVFDA+ER+KAYGF
Sbjct: 313 PTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSYVFDASERAKAYGF 372

Query: 369 TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVSSQH 428
           TS+G++IHVLLLGDIMNFKCRVRSK+K +++EP+A Q IKGYLL+ + EKV V+NVS+QH
Sbjct: 373 TSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVNQEKVFVYNVSTQH 432

Query: 429 YVRVGAPRLLFSAGVDEIKSSFLNY----QNLDLESVGNFIPL-SSDREKLVVLGLGGGY 488
           YVR   PRLLF A +++I+S+FL++    +  D + +    PL +SDREKL+V+GLG GY
Sbjct: 433 YVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASDREKLLVMGLGDGY 492

Query: 489 VGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPT 548
           V  Y+S LPI K EFNTMLW+SPV FFILFLFGAWHFF+KKKE+LT+WGPDDPF++T  T
Sbjct: 493 VATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTAWGPDDPFSST--T 552

Query: 549 TGAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGY-PGGATSSFRPAT 608
             +   T +   ++F ++  R+ + MDLR         RRY SP+ Y PG AT ++R   
Sbjct: 553 MSSSSTTTAQNSSAFSESTRRNDDHMDLR---------RRYVSPSRYPPGAATGAYRSVG 612

Query: 609 TSDHNSKPAAVDPNYR-AASELKFR-GSPLEPPGFPKRREPLFANNQVVN 646
           ++D +S+      NYR  A E+K+R GS L+  GF KRRE LF NN+ ++
Sbjct: 613 SNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKALD 614

BLAST of PI0016300 vs. ExPASy TrEMBL
Match: A0A0A0LNK9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361500 PE=4 SV=1)

HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 621/653 (95.10%), Postives = 634/653 (97.09%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSEIEVPFEIKQYDLLKNLEELV 60
           MAIERKGRFFFIFFL IFAFPHVSRVFVCSSSS EQNQSEIEVPFEIKQYDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSSSLEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLDN 120
           RNLSDVVSKLELRLSDIPTAVNREKLNTDA RRVVPEVGKEK KGKE+GLNDGGDGGLD+
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDAFRRVVPEVGKEKRKGKEEGLNDGGDGGLDH 120

Query: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDER 180
           KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLE+DATCINVLPLRDFEGHSKYVAVGDER
Sbjct: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLEADATCINVLPLRDFEGHSKYVAVGDER 180

Query: 181 GRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSNG 240
           GRIYVFV NGDVAIELPTVP SP+TAMLSYMSIYKNETLLVTGHKNG ILMHRIWEGSNG
Sbjct: 181 GRIYVFVINGDVAIELPTVPGSPVTAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSNG 240

Query: 241 EDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTVYG 300
           EDLNLIFMEHVVEFVAT+SREDESQISLLELHHVGRTRYILSSDF GKIKVF+EDGTVYG
Sbjct: 241 EDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIKVFREDGTVYG 300

Query: 301 SVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSKA 360
           SVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDA ERSKA
Sbjct: 301 SVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAMERSKA 360

Query: 361 YGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVS 420
           YG TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVS
Sbjct: 361 YGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVS 420

Query: 421 SQHYVRVGAPRLLFSAGVDEIKSSFLNYQNLDLESVGNFIPL-SSDREKLVVLGLGGGYV 480
           SQHYVRVGAPRLLFSAG+DEIKSSFLNYQN DL S  NFIPL SSD EKLVVLGLGGGYV
Sbjct: 421 SQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKLVVLGLGGGYV 480

Query: 481 GMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTT 540
           GMYRSNLPIFKGEFNTM+WTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTT
Sbjct: 481 GMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTT 540

Query: 541 GAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPATTS 600
           GAPMGTGSSERASFIDTPSRST+VMDLR  GGLRGPPRRYGSPTGYP GATSSFRPATT+
Sbjct: 541 GAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGATSSFRPATTN 600

Query: 601 DHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDNS 653
           DH+S+PAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDD+S
Sbjct: 601 DHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSS 653

BLAST of PI0016300 vs. ExPASy TrEMBL
Match: A0A1S3BAX2 (uncharacterized membrane protein At1g75140-like OS=Cucumis melo OX=3656 GN=LOC103487958 PE=4 SV=1)

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 625/656 (95.27%), Postives = 634/656 (96.65%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSEIEVPFEIKQYDLLKNLEELV 60
           MAIERKGRFFFIFFL IFAFPHVSRVFVCSS+SFEQNQSEIEVPFEIKQYDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSNSFEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLDN 120
           RNLSDVVSKLELRLSDIPTAVNREKLN DASRRVVPEVGKEKGKGKED   D  D GLD+
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNIDASRRVVPEVGKEKGKGKED---DPNDVGLDH 120

Query: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDER 180
           KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATC NVLPLRDFEG+SKYVAVGDER
Sbjct: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCTNVLPLRDFEGNSKYVAVGDER 180

Query: 181 GRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSNG 240
           GRIYVFVRNGDVAIELPTVP SPITAMLSYMSIYKNETLLVTGHKNG ILMHRIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAIELPTVPGSPITAMLSYMSIYKNETLLVTGHKNGGILMHRIWEGSNG 240

Query: 241 EDLNLIFMEHVVEFVATDSREDE--SQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTV 300
           EDLNLIFME VVEFVATDSREDE  SQISLLELHHVGRTRYILSSD RGKIKVFKEDGTV
Sbjct: 241 EDLNLIFMERVVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGKIKVFKEDGTV 300

Query: 301 YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERS 360
           YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSL RNYVFDATERS
Sbjct: 301 YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVRNYVFDATERS 360

Query: 361 KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN 420
           KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN
Sbjct: 361 KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN 420

Query: 421 VSSQHYVRVGAPRLLFSAGVDEIKSSFLNYQNLDLESVGNF-IPL-SSDREKLVVLGLGG 480
           VSS HYVRVGAPRLLFSAG+DEIKSSFLNYQNLDLES  NF IPL SSDREKLVVLGLGG
Sbjct: 421 VSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLESEANFYIPLISSDREKLVVLGLGG 480

Query: 481 GYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATS 540
           GYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATS
Sbjct: 481 GYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATS 540

Query: 541 PTTGAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPA 600
           PTTGAPMGTGSSERASFIDTPSRST+VMDLRGGGGLRGP RRYGSPTGYPGGATSSFRPA
Sbjct: 541 PTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYPGGATSSFRPA 600

Query: 601 TTSDHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDNS 653
           TTSDH+S+PAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDD++
Sbjct: 601 TTSDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSN 653

BLAST of PI0016300 vs. ExPASy TrEMBL
Match: A0A5A7VC92 (Putative membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005890 PE=4 SV=1)

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 603/658 (91.64%), Postives = 613/658 (93.16%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSEIEVPFEIKQYDLLKNLEELV 60
           MAIERKG                      SS+SFEQNQSEIEVPFEIKQYDLLKNLEELV
Sbjct: 1   MAIERKG----------------------SSNSFEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLDN 120
           RNLSDVVSKLELRLSDIPTAVNREKLN DASRRVVPEVGKEKGKGKED   D  D GLD+
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNIDASRRVVPEVGKEKGKGKED---DPNDVGLDH 120

Query: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDER 180
           KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEG+SKYVAVGDER
Sbjct: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGNSKYVAVGDER 180

Query: 181 GRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSNG 240
           GRIYVFVRNGDVAIELPTVP SPITAMLSYMSIYKNETLLVTGHKNG ILMHRIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAIELPTVPGSPITAMLSYMSIYKNETLLVTGHKNGGILMHRIWEGSNG 240

Query: 241 EDLNLIFMEHVVEFVATDSREDE--SQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTV 300
           EDLNLIFME +VEFVATDSREDE  SQISLLELHHVGRTRYILSSD RGKIKVFKEDGTV
Sbjct: 241 EDLNLIFME-LVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGKIKVFKEDGTV 300

Query: 301 YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERS 360
           YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSL RNYVFDATERS
Sbjct: 301 YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVRNYVFDATERS 360

Query: 361 KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN 420
           KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN
Sbjct: 361 KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN 420

Query: 421 VSSQHYVRVGAPRLLFSAGVDEIKSSFLNYQN--LDLESVGNF-IPL-SSDREKLVVLGL 480
           VSS HYVRVGAPRLLFSAG+DEIKSSFLNYQN  LDLES  NF IPL SSDREKLVVLGL
Sbjct: 421 VSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLDLESEANFYIPLISSDREKLVVLGL 480

Query: 481 GGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540
           G GYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA
Sbjct: 481 GAGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540

Query: 541 TSPTTGAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATSSFR 600
           TSPTTGAPMGTGSSERASFIDTPSRST+VMDLRGGGGLRGP RRYGSPTGYPGGATSSFR
Sbjct: 541 TSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYPGGATSSFR 600

Query: 601 PATTSDHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDNS 653
           PATTSDH+S+PAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDD++
Sbjct: 601 PATTSDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSN 632

BLAST of PI0016300 vs. ExPASy TrEMBL
Match: A0A6J1CSU7 (uncharacterized membrane protein At1g75140-like OS=Momordica charantia OX=3673 GN=LOC111013986 PE=4 SV=1)

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 564/652 (86.50%), Postives = 599/652 (91.87%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQ----SEIEVPFEIKQYDLLKNL 60
           MA  RKG+   I+FL +F  PHVSRVFVCSSSSFEQNQ    SE EVPFEI+Q DLLKNL
Sbjct: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60

Query: 61  EELVRNLSDVVSKLELRLSDIPTAVNREKL-NTDASRRVVPEVGKEKGKGKEDGLNDGGD 120
           EELVRNLSDVVSKLE+RLSDIPT V R+K  NTDASRRVVPEV    G+ KEDGLNDGGD
Sbjct: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSLGGR-KEDGLNDGGD 120

Query: 121 GGLDNKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVA 180
           GGL++KI+DG RTRAVSVTKYS FWSERFHFLSAVKL+SDATCINVLP RDFEGHSKYV 
Sbjct: 121 GGLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFEGHSKYVV 180

Query: 181 VGDERGRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIW 240
           VGDE+GR+YVF RNGDVA+EL T  ESPI  MLSYMSIYKNETLLVTGHKNG ILMHRIW
Sbjct: 181 VGDEKGRVYVFTRNGDVAVELRTKSESPIMTMLSYMSIYKNETLLVTGHKNGAILMHRIW 240

Query: 241 EGSNGEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDFRGKIKVFKED 300
           E SNGE+ + +FMEHVVEFVATD REDES IS+LE+HHVGRTRYILSSD RGKIKVF+E+
Sbjct: 241 ERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIKVFREN 300

Query: 301 GTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAT 360
           GTVYGSVMP S+PIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLN+SLARNYVFDAT
Sbjct: 301 GTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDAT 360

Query: 361 ERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVH 420
           ERSKAYGFTS GDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ IKGYLLV SNEKVH
Sbjct: 361 ERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVH 420

Query: 421 VFNVSSQHYVRVGAPRLLFSAGVDEIKSSFLNYQNLDLESVGNFIPL-SSDREKLVVLGL 480
           +FNVSSQHY RVGAPR LFSA VDEI+SSFLNYQNLDLES G F+PL SSDREKLVVLGL
Sbjct: 421 IFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKLVVLGL 480

Query: 481 GGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540
           GGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA
Sbjct: 481 GGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540

Query: 541 TSPTTGAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATSSFR 600
           TSPTTGAP+GTG+SER+SFIDTPSRST++MDLRGG GLRGPPRRYGSPTGYPGGAT+SFR
Sbjct: 541 TSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGATNSFR 600

Query: 601 PATTSDHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQ 647
           P TTSDHNS+PAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV++
Sbjct: 601 PPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDE 651

BLAST of PI0016300 vs. ExPASy TrEMBL
Match: A0A6J1KBU3 (uncharacterized membrane protein At1g75140-like OS=Cucurbita maxima OX=3661 GN=LOC111491879 PE=4 SV=1)

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 566/668 (84.73%), Postives = 603/668 (90.27%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSEIEVPFEIKQYDLLKNLEELV 60
           MA  RKG+  FI+FL +F  PHVSR FVC SSSFE NQSE EVPFE +QYDLLKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPHVSRDFVCCSSSFELNQSETEVPFETRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVNRE-KLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLD 120
           RNLSDVVSKLELRLS+IPTAV+R+ KLNTDASRRVVPEV    G GKEDGLNDGGDG LD
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEV-VSLGGGKEDGLNDGGDGALD 120

Query: 121 NKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDE 180
            KIQDGGR RAVSVTKYS FWSERFHFLSAVKLESDAT INVLPLRDFEGHSKYVAVGDE
Sbjct: 121 TKIQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSN 240
            GRIYVFVRNGDVA+EL T+  SPITA LSYMSIYKNETLLVTGH++G I M RIWEGSN
Sbjct: 181 TGRIYVFVRNGDVAVELRTMSGSPITATLSYMSIYKNETLLVTGHESGAISMLRIWEGSN 240

Query: 241 GEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTVY 300
           GE+ N IFMEHV+EFV TD RE++S IS+LE+HHVGRTRYILSSD +GKIKVF+EDGTVY
Sbjct: 241 GEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTVY 300

Query: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSK 360
           GSVMPTSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLN+SLARNYVFDATERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSK 360

Query: 361 AYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNV 420
           AYGFTSDGDLIHV+LLGDIMNFKCRVRSK+KFELDEPL FQ +KGYLLV  +EKVHVFNV
Sbjct: 361 AYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFNV 420

Query: 421 SSQHYVRVGAPRLLFSAGVDEIKSSFLNYQNLDLESVGNFIPL-SSDREKLVVLGLGGGY 480
           SSQHYVRVGAPRLLFSA +DEI+SSFLN QNLDLES G FIPL SSDREKLVVLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGGY 480

Query: 481 VGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPT 540
           +GMYRSNLPI+KGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTATSPT
Sbjct: 481 IGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 TGAPMGTGSSE--------------RASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTG 600
           TGAP+GTGS+E              R SFIDTPSRST++MDLRGG GLRGPPRRYGSPTG
Sbjct: 541 TGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTG 600

Query: 601 YPGGATSSFRPATTSDHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV 653
           YPGG TSSFRPATTSDH+S+ AAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV
Sbjct: 601 YPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV 660

BLAST of PI0016300 vs. NCBI nr
Match: XP_011649613.2 (uncharacterized membrane protein At1g75140 [Cucumis sativus] >KAE8652103.1 hypothetical protein Csa_022548 [Cucumis sativus])

HSP 1 Score: 1230.3 bits (3182), Expect = 0.0e+00
Identity = 622/653 (95.25%), Postives = 635/653 (97.24%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSEIEVPFEIKQYDLLKNLEELV 60
           MAIERKGRFFFIFFL IFAFPHVSRVFVCSSSS EQNQSEIEVPFEIKQYDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSSSLEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLDN 120
           RNLSDVVSKLELRLSDIPTAVNREKLNTDA RRVVPEVGKEK KGKE+GLNDGGDGGLD+
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDAFRRVVPEVGKEKRKGKEEGLNDGGDGGLDH 120

Query: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDER 180
           KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLE+DATCINVLPLRDFEGHSKYVAVGDER
Sbjct: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLEADATCINVLPLRDFEGHSKYVAVGDER 180

Query: 181 GRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSNG 240
           GRIYVFVRNGDVAIELPTVP SP+TAMLSYMSIYKNETLLVTGHKNG ILMHRIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAIELPTVPGSPVTAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSNG 240

Query: 241 EDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTVYG 300
           EDLNLIFMEHVVEFVAT+SREDESQISLLELHHVGRTRYILSSDF GKIKVF+EDGTVYG
Sbjct: 241 EDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIKVFREDGTVYG 300

Query: 301 SVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSKA 360
           SVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDA ERSKA
Sbjct: 301 SVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAMERSKA 360

Query: 361 YGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVS 420
           YG TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVS
Sbjct: 361 YGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVS 420

Query: 421 SQHYVRVGAPRLLFSAGVDEIKSSFLNYQNLDLESVGNFIPL-SSDREKLVVLGLGGGYV 480
           SQHYVRVGAPRLLFSAG+DEIKSSFLNYQN DL S  NFIPL SSD EKLVVLGLGGGYV
Sbjct: 421 SQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKLVVLGLGGGYV 480

Query: 481 GMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTT 540
           GMYRSNLPIFKGEFNTM+WTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTT
Sbjct: 481 GMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTT 540

Query: 541 GAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPATTS 600
           GAPMGTGSSERASFIDTPSRST+VMDLR  GGLRGPPRRYGSPTGYP GATSSFRPATT+
Sbjct: 541 GAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGATSSFRPATTN 600

Query: 601 DHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDNS 653
           DH+S+PAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDD+S
Sbjct: 601 DHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSS 653

BLAST of PI0016300 vs. NCBI nr
Match: XP_008444699.1 (PREDICTED: uncharacterized membrane protein At1g75140-like [Cucumis melo])

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 625/656 (95.27%), Postives = 634/656 (96.65%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSEIEVPFEIKQYDLLKNLEELV 60
           MAIERKGRFFFIFFL IFAFPHVSRVFVCSS+SFEQNQSEIEVPFEIKQYDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSNSFEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLDN 120
           RNLSDVVSKLELRLSDIPTAVNREKLN DASRRVVPEVGKEKGKGKED   D  D GLD+
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNIDASRRVVPEVGKEKGKGKED---DPNDVGLDH 120

Query: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDER 180
           KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATC NVLPLRDFEG+SKYVAVGDER
Sbjct: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCTNVLPLRDFEGNSKYVAVGDER 180

Query: 181 GRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSNG 240
           GRIYVFVRNGDVAIELPTVP SPITAMLSYMSIYKNETLLVTGHKNG ILMHRIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAIELPTVPGSPITAMLSYMSIYKNETLLVTGHKNGGILMHRIWEGSNG 240

Query: 241 EDLNLIFMEHVVEFVATDSREDE--SQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTV 300
           EDLNLIFME VVEFVATDSREDE  SQISLLELHHVGRTRYILSSD RGKIKVFKEDGTV
Sbjct: 241 EDLNLIFMERVVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGKIKVFKEDGTV 300

Query: 301 YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERS 360
           YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSL RNYVFDATERS
Sbjct: 301 YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVRNYVFDATERS 360

Query: 361 KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN 420
           KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN
Sbjct: 361 KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN 420

Query: 421 VSSQHYVRVGAPRLLFSAGVDEIKSSFLNYQNLDLESVGNF-IPL-SSDREKLVVLGLGG 480
           VSS HYVRVGAPRLLFSAG+DEIKSSFLNYQNLDLES  NF IPL SSDREKLVVLGLGG
Sbjct: 421 VSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLESEANFYIPLISSDREKLVVLGLGG 480

Query: 481 GYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATS 540
           GYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATS
Sbjct: 481 GYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATS 540

Query: 541 PTTGAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPA 600
           PTTGAPMGTGSSERASFIDTPSRST+VMDLRGGGGLRGP RRYGSPTGYPGGATSSFRPA
Sbjct: 541 PTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYPGGATSSFRPA 600

Query: 601 TTSDHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDNS 653
           TTSDH+S+PAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDD++
Sbjct: 601 TTSDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSN 653

BLAST of PI0016300 vs. NCBI nr
Match: XP_038885478.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 588/656 (89.63%), Postives = 618/656 (94.21%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSEIEVPFEIKQYDLLKNLEELV 60
           MA  RKG+  FI+FL +F+FPHVSRVFVCSSSSFE NQSEIEVPFEIKQY+LLKNLEELV
Sbjct: 1   MANLRKGKLLFIYFLFVFSFPHVSRVFVCSSSSFELNQSEIEVPFEIKQYNLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEV---GKEKGKGKEDGLNDGGDGG 120
           RNLSDVVSKLELRLSDIPTAV+++KLNTDASRRVV EV   G  K KGKEDGLNDGGDGG
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVHKDKLNTDASRRVVSEVVGLGGVKRKGKEDGLNDGGDGG 120

Query: 121 LDNKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVG 180
           LDNKIQDGGR RAVSVTKYS FWSERFHFLSAVKLESDATCINVLP+RDFEGHSKYVAVG
Sbjct: 121 LDNKIQDGGRMRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPIRDFEGHSKYVAVG 180

Query: 181 DERGRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEG 240
           DE+GRIYVFVRNGDVAIELPTV ESPITAMLSY SIYKNETLLVTGH NG ILMHRIWEG
Sbjct: 181 DEKGRIYVFVRNGDVAIELPTVSESPITAMLSYTSIYKNETLLVTGHNNGAILMHRIWEG 240

Query: 241 SNGEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDFRGKIKVFKEDGT 300
            NGE+LN IFMEHVVEFVATDSRED+S IS+LE+HHVGRTRYILSSDFRGKIKVFKEDGT
Sbjct: 241 LNGEELNSIFMEHVVEFVATDSREDDSPISILEVHHVGRTRYILSSDFRGKIKVFKEDGT 300

Query: 301 VYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATER 360
           VYGSV   SRPIAFLKQRLLFLTESGAGSLDLRSMKLRE+ CEGLN+SLARNYVFDATER
Sbjct: 301 VYGSVTLMSRPIAFLKQRLLFLTESGAGSLDLRSMKLREAGCEGLNYSLARNYVFDATER 360

Query: 361 SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVF 420
           SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ IKGYLLV SNEKVHVF
Sbjct: 361 SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHVF 420

Query: 421 NVSSQHYVRVGAPRLLFSAGVDEIKSSFLNYQNLDLESVGNFIPL-SSDREKLVVLGLGG 480
           NVSSQHYVRV APRLLFSAG+DEI+SSFLNYQNL+LES G FIPL SSD EKLVVLGLGG
Sbjct: 421 NVSSQHYVRVAAPRLLFSAGLDEIRSSFLNYQNLNLESEGKFIPLISSDHEKLVVLGLGG 480

Query: 481 GYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATS 540
           GYVGMYRSNLPIFKGEFN+MLWTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPFTATS
Sbjct: 481 GYVGMYRSNLPIFKGEFNSMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATS 540

Query: 541 PTTGAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPA 600
           PTTG P+GTGSSERASFIDTPSRST++MDLRGGGG+RGPPR YGSPTGYPGGATSSFRP 
Sbjct: 541 PTTGVPLGTGSSERASFIDTPSRSTDMMDLRGGGGIRGPPRHYGSPTGYPGGATSSFRPP 600

Query: 601 TTSDHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDNS 653
           TTSDH+S+PAAVDP+YRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQ+VD++S
Sbjct: 601 TTSDHSSRPAAVDPSYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQMVDESS 656

BLAST of PI0016300 vs. NCBI nr
Match: KAA0065248.1 (putative membrane protein [Cucumis melo var. makuwa])

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 603/658 (91.64%), Postives = 613/658 (93.16%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSEIEVPFEIKQYDLLKNLEELV 60
           MAIERKG                      SS+SFEQNQSEIEVPFEIKQYDLLKNLEELV
Sbjct: 1   MAIERKG----------------------SSNSFEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLDN 120
           RNLSDVVSKLELRLSDIPTAVNREKLN DASRRVVPEVGKEKGKGKED   D  D GLD+
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNIDASRRVVPEVGKEKGKGKED---DPNDVGLDH 120

Query: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDER 180
           KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEG+SKYVAVGDER
Sbjct: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGNSKYVAVGDER 180

Query: 181 GRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSNG 240
           GRIYVFVRNGDVAIELPTVP SPITAMLSYMSIYKNETLLVTGHKNG ILMHRIWEGSNG
Sbjct: 181 GRIYVFVRNGDVAIELPTVPGSPITAMLSYMSIYKNETLLVTGHKNGGILMHRIWEGSNG 240

Query: 241 EDLNLIFMEHVVEFVATDSREDE--SQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTV 300
           EDLNLIFME +VEFVATDSREDE  SQISLLELHHVGRTRYILSSD RGKIKVFKEDGTV
Sbjct: 241 EDLNLIFME-LVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGKIKVFKEDGTV 300

Query: 301 YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERS 360
           YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSL RNYVFDATERS
Sbjct: 301 YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVRNYVFDATERS 360

Query: 361 KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN 420
           KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN
Sbjct: 361 KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFN 420

Query: 421 VSSQHYVRVGAPRLLFSAGVDEIKSSFLNYQN--LDLESVGNF-IPL-SSDREKLVVLGL 480
           VSS HYVRVGAPRLLFSAG+DEIKSSFLNYQN  LDLES  NF IPL SSDREKLVVLGL
Sbjct: 421 VSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLDLESEANFYIPLISSDREKLVVLGL 480

Query: 481 GGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540
           G GYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA
Sbjct: 481 GAGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTA 540

Query: 541 TSPTTGAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATSSFR 600
           TSPTTGAPMGTGSSERASFIDTPSRST+VMDLRGGGGLRGP RRYGSPTGYPGGATSSFR
Sbjct: 541 TSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYPGGATSSFR 600

Query: 601 PATTSDHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDNS 653
           PATTSDH+S+PAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDD++
Sbjct: 601 PATTSDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSN 632

BLAST of PI0016300 vs. NCBI nr
Match: XP_038885660.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 579/653 (88.67%), Postives = 608/653 (93.11%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSEIEVPFEIKQYDLLKNLEELV 60
           MA  RKG+  FI+ LL+FAF HVSRV VCSSSSFE NQS IEVPFE++QYDLLKNLEELV
Sbjct: 1   MANPRKGKLLFIYLLLVFAFLHVSRVVVCSSSSFELNQSGIEVPFEVRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLDN 120
           RNLSDVVSKLELRLSDIPT V REKLN DASRRV+PE          D LNDGGDGGLDN
Sbjct: 61  RNLSDVVSKLELRLSDIPTVVRREKLNPDASRRVMPE---------GDTLNDGGDGGLDN 120

Query: 121 KIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDER 180
           KIQDG R RAVSVTKYS FWSERFHFLSAVKLESDATCINVLPLRDFEG+SKYVAVGDE+
Sbjct: 121 KIQDGSRARAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFEGNSKYVAVGDEK 180

Query: 181 GRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSNG 240
           GRIYVF+R+GDVAIELPTV ESPITAMLSYMSIYKNETLLVTGHKNG+ILMHRIWEGSNG
Sbjct: 181 GRIYVFMRSGDVAIELPTVSESPITAMLSYMSIYKNETLLVTGHKNGMILMHRIWEGSNG 240

Query: 241 EDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTVYG 300
           E+LNLIFMEHVVEF  TDS+EDES IS+LE+HHVG TRYILSSDFRG IKVFKEDGTVYG
Sbjct: 241 EELNLIFMEHVVEFADTDSQEDESPISILEVHHVGWTRYILSSDFRGVIKVFKEDGTVYG 300

Query: 301 SVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSKA 360
           SVMP SRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLN+SLARNYVFDATERSKA
Sbjct: 301 SVMPMSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSKA 360

Query: 361 YGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVS 420
           YGFTSDGDLIHVLLLGDIMNFKCR+RSKRKFELD PL FQ IKGYLLV SNEKVH+FNVS
Sbjct: 361 YGFTSDGDLIHVLLLGDIMNFKCRIRSKRKFELDGPLVFQAIKGYLLVISNEKVHIFNVS 420

Query: 421 SQHYVRVGAPRLLFSAGVDEIKSSFLNYQNLDLESVGNFIPL-SSDREKLVVLGLGGGYV 480
           SQHYVRVGAPRLLFSAG+DEI SSFLNYQNLDLES GNFIPL SSDREKLVVLGLGGGYV
Sbjct: 421 SQHYVRVGAPRLLFSAGLDEIMSSFLNYQNLDLESEGNFIPLISSDREKLVVLGLGGGYV 480

Query: 481 GMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTT 540
           GMYRSNLP+FKGEFNTMLWTSPVLFFILFLFGAWH FAKKKEALTSWGPDD FTATSPTT
Sbjct: 481 GMYRSNLPVFKGEFNTMLWTSPVLFFILFLFGAWHLFAKKKEALTSWGPDDSFTATSPTT 540

Query: 541 GAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPATTS 600
           GAP+GTGSSERASFID PSR+T++MDLRGG GLRGPPRRYGSPTGYPGGATSSFRPATTS
Sbjct: 541 GAPLGTGSSERASFIDPPSRTTDMMDLRGGAGLRGPPRRYGSPTGYPGGATSSFRPATTS 600

Query: 601 DHNSKPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDNS 653
           DH+S+PAAVDPNYRAASE+KFRGSPLEPPGFPKRREPLFANNQVVNQVV +N+
Sbjct: 601 DHSSRPAAVDPNYRAASEIKFRGSPLEPPGFPKRREPLFANNQVVNQVVAENN 644

BLAST of PI0016300 vs. TAIR 10
Match: AT1G75140.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G19370.1); Has 51 Blast hits to 49 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 644.0 bits (1660), Expect = 1.3e-184
Identity = 352/650 (54.15%), Postives = 453/650 (69.69%), Query Frame = 0

Query: 9   FFFIFFLLIFAFPHVSRVFVCSSSSFEQNQSE-----IEVPFEIKQYDLLKNLEELVRNL 68
           FFF+  +L+F  P  S V    S       S+     +      +Q   L  LEELVRNL
Sbjct: 13  FFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGVSLHRLEELVRNL 72

Query: 69  SDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDGGLDNKIQ 128
           +++V++L+ +LS+ P  V +E         +  +  +EK K                   
Sbjct: 73  TELVARLDAKLSETPFKVKKE---------ITRDEIEEKAK------------------- 132

Query: 129 DGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDERGRI 188
                 A SVTKYS FWSERF F SAVKL+S+ATCINVLP RD EG SKY AVGD  GR+
Sbjct: 133 ------AFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGLSKYFAVGDSSGRV 192

Query: 189 YVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWEGSNGEDL 248
           +VF+RNGDV +E  T  +SPITAM+SYMS+YKNE+ +VTGH++GVIL+HR+ EGS GEDL
Sbjct: 193 FVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVILLHRLREGSIGEDL 252

Query: 249 NLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDFRGKIKVFKEDGTVYGSVM 308
           N   ME+V +F   D  ED  Q++LLE+HHVGR RYIL++D  GK+ VF E+ TVYGSV 
Sbjct: 253 NSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLTVFTENRTVYGSVS 312

Query: 309 PTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSKAYGF 368
           PTSRP+ FLKQRLLFLTE+GAGSLDLRSMK+RESECEGLNHSLAR+YVFDA+ER+KAYGF
Sbjct: 313 PTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSYVFDASERAKAYGF 372

Query: 369 TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVSSQH 428
           TS+G++IHVLLLGDIMNFKCRVRSK+K +++EP+A Q IKGYLL+ + EKV V+NVS+QH
Sbjct: 373 TSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVNQEKVFVYNVSTQH 432

Query: 429 YVRVGAPRLLFSAGVDEIKSSFLNY----QNLDLESVGNFIPL-SSDREKLVVLGLGGGY 488
           YVR   PRLLF A +++I+S+FL++    +  D + +    PL +SDREKL+V+GLG GY
Sbjct: 433 YVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASDREKLLVMGLGDGY 492

Query: 489 VGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPT 548
           V  Y+S LPI K EFNTMLW+SPV FFILFLFGAWHFF+KKKE+LT+WGPDDPF++T  T
Sbjct: 493 VATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTAWGPDDPFSST--T 552

Query: 549 TGAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGY-PGGATSSFRPAT 608
             +   T +   ++F ++  R+ + MDLR         RRY SP+ Y PG AT ++R   
Sbjct: 553 MSSSSTTTAQNSSAFSESTRRNDDHMDLR---------RRYVSPSRYPPGAATGAYRSVG 612

Query: 609 TSDHNSKPAAVDPNYR-AASELKFR-GSPLEPPGFPKRREPLFANNQVVN 646
           ++D +S+      NYR  A E+K+R GS L+  GF KRRE LF NN+ ++
Sbjct: 613 SNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKALD 614

BLAST of PI0016300 vs. TAIR 10
Match: AT1G19370.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G75140.1); Has 45 Blast hits to 43 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 632.1 bits (1629), Expect = 4.9e-181
Identity = 350/658 (53.19%), Postives = 453/658 (68.84%), Query Frame = 0

Query: 1   MAIERKGRFFFIFFLLIFAFP--HVSRVFVCSSSSFEQNQSEIEVPFE--IKQYDLLKNL 60
           MA    G+ FFI F L+F+F    +S ++   S   E   +++ V      ++  LL NL
Sbjct: 1   MADPLNGKSFFICFSLLFSFTLLFISPLYATESPVIEDVSTDVAVSVSETNREAVLLHNL 60

Query: 61  EELVRNLSDVVSKLELRLSDIPTAVNREKLNTDASRRVVPEVGKEKGKGKEDGLNDGGDG 120
           EELV+NL+++V+ L+ +LS  P    + +++ D       ++G+EK              
Sbjct: 61  EELVKNLTELVANLDAKLSATPLK-EKNEISVD------DDIGEEK-------------- 120

Query: 121 GLDNKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAV 180
                  + GR +A SVTKYS FWSERF F SAVKL SDATCINVLP RDFEG SKY A+
Sbjct: 121 -------ERGRAKAFSVTKYSPFWSERFQFTSAVKLNSDATCINVLPFRDFEGSSKYFAI 180

Query: 181 GDERGRIYVFVRNGDVAIELPTVPESPITAMLSYMSIYKNETLLVTGHKNGVILMHRIWE 240
           GD +GR+YVF+RNGDV IE  T  +SP+TAM+SY S++KN + +VTGH+NG +L+HRI E
Sbjct: 181 GDSKGRVYVFLRNGDVLIEFFTTVDSPVTAMVSYSSVFKNSSFVVTGHQNGAVLLHRIHE 240

Query: 241 GSNGED--LNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDFRGKIKVFKE 300
           GSNGED   N + MEHV +F   DS +    ++LLE+HHVGR RYIL++D  GK+ V  E
Sbjct: 241 GSNGEDWNSNSVSMEHVGKFDVDDSAD---PVTLLEVHHVGRVRYILATDLSGKLTVLTE 300

Query: 301 DGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDA 360
           + TVYGSV+P+SRP+ FLKQRLLFLTESGAGSLDLRSMK+RE+ECEGLNHSLAR YVFDA
Sbjct: 301 NRTVYGSVIPSSRPLVFLKQRLLFLTESGAGSLDLRSMKIRETECEGLNHSLARTYVFDA 360

Query: 361 TERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKV 420
            ERSKAYGFTS+G++IHVLL GDIMNFKCRVRSK+KF+++EP+A Q+IKGYLLV + EKV
Sbjct: 361 AERSKAYGFTSEGEIIHVLLHGDIMNFKCRVRSKKKFQMEEPVALQSIKGYLLVINEEKV 420

Query: 421 HVFNVSSQHYVRVGAPRLLFSAGVDEIKSSFLNYQNLD--LESVGNFIPL-SSDREKLVV 480
             FNVS+QHYVR   PRLLFSAG++EI+S+FL+++       +V    PL +SDRE L+V
Sbjct: 421 FAFNVSTQHYVRTAGPRLLFSAGLEEIRSAFLSHRESSSRTTTVVKTRPLIASDRENLLV 480

Query: 481 LGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDP 540
           +GL  GY  +Y+S LP  KG+FNTMLW+SPV FFILFLFGAWHFFAKKKE+LT+WGPDDP
Sbjct: 481 IGLENGYFAVYKSKLPTLKGDFNTMLWSSPVFFFILFLFGAWHFFAKKKESLTAWGPDDP 540

Query: 541 FTATSPTTGAPMGTGSSERASFIDTPSRSTEVMDLRGGGGLRGPPRRYGSPTGYPGGATS 600
           FT     TGA     S++  +F +   R+ ++MDLR         RRY         A  
Sbjct: 541 FT----PTGAQ--NSSAKEPTFTEPSRRNDDLMDLR---------RRY---------AGG 600

Query: 601 SFRPATTSDHNSKPAAVDPNYRAASE----LKFRGSPLEPPGFPKRREPLFANNQVVN 646
           S+R    +D +S+ A VD NYR  ++     +  GS L+  GF  RR+ LF NN+V++
Sbjct: 601 SYRSVGANDPSSR-APVDGNYRTTAQDHNNYRGGGSGLDSNGFGNRRDHLFGNNKVLD 602