PI0016022 (gene) Melon (PI 482460) v1

Overview


Name	PI0016022
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	chr10: 20906669 .. 20909469 (-)
RNA-Seq Expression	PI0016022
Synteny	PI0016022

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGAAAAGCAATTTGGACGTAACGTGTGCTAAACCACACCACTGGTTCTTCTTGTACACAATGAAAAGAAGCTTCAATCCCCAAATCCCAAAAATGGATAAATCCCAATGGTCTTCTATAAATCCTCAATCTCCCACCCTCCTATGTCGTTTCAGATTCTTACCCACATCTTCTGAAGCTCTTTCCCATCTGGGTTTTGGGGACGATGGATGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCACGTTCGTCAATGTGATGACGGTGGTCAGCTCGACGTCGTTGGCCATGGCTGGTCTTTTCGAGATAAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCAAATAGTGGTCCGAAACTTTCAGCCAAAGAATCTAAGCTTTCGTCCAAATACGGAATGCTTTTGGCTTATACTCCGGCGTTTCTAGCTGGTGCGGCGTCGTTTTGGCTCTTTCCGAGCGGTGATGAGAGGTTTTTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTTGAGGTACTCTCTCTCTCTCTCTCTCTCTCTTTAAGCCTTTGTTTGAGTTCTGAAATTTCAAATCTGATTCTGGAATTAGGGATTACAGAATGCAGTTCTTCTCTGATGTTCTTGAGTATAGCGAAATTCATATCCTCGTAAAATTATTTGTTCGATTCGATTTGGTGTTTATTGAGAAGTTGTTAATCTTATTCTTCAAAAATTTCTTGTGTTCTTGAAAACCGTATGAAAATTCGCGGCGAAGAGAAAGTTGTAGAGAGATCTGCATCTCTTTTTCCCTCCCTGCTTCTTATCATTCCTTTGGAGATTTCAAGTTTGGAACTCTTTTCTTCTCTGTTTACGGGATTGAAAAACTTTTGACCCAACTTTTTTTTTTTTTTAATTCGTTGTTTGTATTATGAATTAGGGTTTCGAACTTTGACTTAGGCTCACTTTGGCGGTTGTCTTATTATCAATTTTGATGAGTATTTTTGTTCTTTCTTTTGACCATGGATGGCTGAATCAAACCTCGAGTTGGAAGTTGCTAGTTGAGCTATGCCCTTTTAACAGTAACCCATTTGCTTATAAACAATTTGACGTTATTAATTTTGCTCTTTGACTAATTTAATTATCATTAAGATATTATACGAGTGTACTTTGTATAGAATGATTAGACTGATTATTAAATGAAGTCATGCTTAAGAAAGAAACAAACAACACGATTCTGTTCAGCAGTTCTCCAAAAATTCTGTTTCACTAATCACGATGTGATTTGCAAGTCTTATACCTTTCATTCGTGGAACTATTCTTCTTTGTTCTTTATTTTTTTTTTCTTTTTTCACCCCATTGCTGCTGAAAGGTTGAACTATGAAACTTTGAAATAGTAATAGATATCTTGCAAATTATATGAGCTGTTTAGAAAAGAATGCAAAATGTTTTTAAAATTTAAGCTGATGGCCATGTTGAGTTTAATTATTGCAATACTTACCCCCTTTTTCACTTGACAAGTTAGAAATTTTAACATATAAGAGTCTTGAAATGCTTTGAATCTGTTATATGATGCTTCAAATGACTAAGTTCGGAGGCTCACTGTATTTGTTAATACACTATTTACGATTTTAGGCTTAAAGTTTAGAGAGGTGCCATATATAAATTCTCTAACTCTATTAGCATCTCTGGAATTTGTATTATCTGAAATTTCTCTCGAGTCATAATTTGCTTTGTCTCTCTACCCGTGGATGTAGCTTACACAGCTAGTGAACCATGCAAATCTCTATGTCGATATTTATCTACTCGCATTGTTTATTTGTTGAATTTTTAACAAAGAAGTTCAACTACTACTATCTTCGAAAGCTTAAGTTGATGGATACGTATACATTAATTTTAATCAATTTTTTAAATCATCTCTGAAGTAGTGTGTTCTGGAAAATCTTGACAGGTACTGTTTATTCACAAATACAGCAGTAAAATGGTATTGGAGACAGCAATTAAAATCTCCTTCAGCTACTTCATGTCCACAGCAATCATCATATACACTCAACACCTTTCACTAGGCCTTCCTGAGCCACCCGTTGATCTGAAAAACATTGGGGTTGCTTTGTTTCTGATAGGCAGTATTGGGAACTTTTACCATCATTATCTCTTGTCACAAACAAGAAAACAAGGAGAAACAGCCTACAAAATTCCCAAAGGAGGCCTCTTCAGCTTTGTCGTTTGCCCTCATTATCTGTTTGAAATAATTGGGTATTTTGGATTTGCTTTCATTTCTCAGGAACTTTATCCCTTGTGCTTCAGCATTGGCACTGCTATTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAATTTGAAAATTTCCCCAACCATGTCAAAGCTCTTCTCCCTTTTGTCTTTTAGACCCTTTTTCCAAGTTACAAAAAAACAAAAAAGTCATAGTTTAGGAAAAGGAGGTGAGTTTTTTCCTTTCTTTCTTACAAAGATATTGAATATTAAATTAAATATGTGGGGTTGGAAAATTAATCCTTGTAAATGTTATTTCAGTTGAACTATAAAGTTACTTGGCTTATACTTTAGCCAAATAAGTTGGGTTCATACTAGTGTTTTAGGTTATTATTAGTTTTTTACATCATTAGCACTACTATGATAATATATTACTGGTTGATTGTTTTTTTCCCCTTTAAAATACTATTTTGGTCCCAATATTTTGAAAGTTTTTCAGTTTTAGTCAACATATTTTTAATTGTCTAATTCTAATCCTTATAACTTCATAAATTTTAA

mRNA sequence

ACGAAAAGCAATTTGGACGTAACGTGTGCTAAACCACACCACTGGTTCTTCTTGTACACAATGAAAAGAAGCTTCAATCCCCAAATCCCAAAAATGGATAAATCCCAATGGTCTTCTATAAATCCTCAATCTCCCACCCTCCTATGTCGTTTCAGATTCTTACCCACATCTTCTGAAGCTCTTTCCCATCTGGGTTTTGGGGACGATGGATGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCACGTTCGTCAATGTGATGACGGTGGTCAGCTCGACGTCGTTGGCCATGGCTGGTCTTTTCGAGATAAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCAAATAGTGGTCCGAAACTTTCAGCCAAAGAATCTAAGCTTTCGTCCAAATACGGAATGCTTTTGGCTTATACTCCGGCGTTTCTAGCTGGTGCGGCGTCGTTTTGGCTCTTTCCGAGCGGTGATGAGAGGTTTTTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTTGAGGTACTGTTTATTCACAAATACAGCAGTAAAATGGTATTGGAGACAGCAATTAAAATCTCCTTCAGCTACTTCATGTCCACAGCAATCATCATATACACTCAACACCTTTCACTAGGCCTTCCTGAGCCACCCGTTGATCTGAAAAACATTGGGGTTGCTTTGTTTCTGATAGGCAGTATTGGGAACTTTTACCATCATTATCTCTTGTCACAAACAAGAAAACAAGGAGAAACAGCCTACAAAATTCCCAAAGGAGGCCTCTTCAGCTTTGTCGTTTGCCCTCATTATCTGTTTGAAATAATTGGGTATTTTGGATTTGCTTTCATTTCTCAGGAACTTTATCCCTTGTGCTTCAGCATTGGCACTGCTATTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAATTTGAAAATTTCCCCAACCATGTCAAAGCTCTTCTCCCTTTTGTCTTTTAGACCCTTTTTCCAAGTTACAAAAAAACAAAAAAGTCATAGTTTAGGAAAAGGAGGTGAGTTTTTTCCTTTCTTTCTTACAAAGATATTGAATATTAAATTAAATATGTGGGGTTGGAAAATTAATCCTTGTAAATGTTATTTCAGTTGAACTATAAAGTTACTTGGCTTATACTTTAGCCAAATAAGTTGGGTTCATACTAGTGTTTTAGGTTATTATTAGTTTTTTACATCATTAGCACTACTATGATAATATATTACTGGTTGATTGTTTTTTTCCCCTTTAAAATACTATTTTGGTCCCAATATTTTGAAAGTTTTTCAGTTTTAGTCAACATATTTTTAATTGTCTAATTCTAATCCTTATAACTTCATAAATTTTAA

Coding sequence (CDS)

ATGGATGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCACGTTCGTCAATGTGATGACGGTGGTCAGCTCGACGTCGTTGGCCATGGCTGGTCTTTTCGAGATAAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCAAATAGTGGTCCGAAACTTTCAGCCAAAGAATCTAAGCTTTCGTCCAAATACGGAATGCTTTTGGCTTATACTCCGGCGTTTCTAGCTGGTGCGGCGTCGTTTTGGCTCTTTCCGAGCGGTGATGAGAGGTTTTTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATCTTTGAGGTACTGTTTATTCACAAATACAGCAGTAAAATGGTATTGGAGACAGCAATTAAAATCTCCTTCAGCTACTTCATGTCCACAGCAATCATCATATACACTCAACACCTTTCACTAGGCCTTCCTGAGCCACCCGTTGATCTGAAAAACATTGGGGTTGCTTTGTTTCTGATAGGCAGTATTGGGAACTTTTACCATCATTATCTCTTGTCACAAACAAGAAAACAAGGAGAAACAGCCTACAAAATTCCCAAAGGAGGCCTCTTCAGCTTTGTCGTTTGCCCTCATTATCTGTTTGAAATAATTGGGTATTTTGGATTTGCTTTCATTTCTCAGGAACTTTATCCCTTGTGCTTCAGCATTGGCACTGCTATTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAATTTGAAAATTTCCCCAACCATGTCAAAGCTCTTCTCCCTTTTGTCTTTTAG

Protein sequence

MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKESKLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSKMVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLSQTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRSYATRKWYISKFENFPNHVKALLPFVF

Homology

[Close]

BLAST of PI0016022 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.4e-17
Identity = 77/260 (29.62%), Postives = 125/260 (48.08%), Query Frame = 0


Query: 19  VMTVVSSTSLAMAGLFEINGKHLQ-YSKFWNANS-------GPKLSAKESKLSSKYGMLL 78
           +   V S +  +   FE  GK     SK   A+S       GP++S     +    G L 
Sbjct: 44  IRLAVPSKTSKLPNAFEALGKDSDLVSKHVGADSTLYFKDLGPQISWSLVFICEYAGPLF 103

Query: 79  AYTPAFLAGAASFWLFPSGDERFFLLKSAL---TLHFFKRIFEVLFIHKYS-SKMVLETA 138
            Y P F     S  ++ +   + F  K AL   +LH+ KRI+E +F+H++S   M +   
Sbjct: 104 VY-PIFY--FLSNLIYGTDSPKSFAQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNL 163

Query: 139 IKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLSQTRKQG 198
            K    Y+  TA++ Y  +  L   E P++   +G+ L++IG + N+  H  L   R  G
Sbjct: 164 FKNCSYYWGCTAMVSYFVNHPL-YTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLRPAG 223

Query: 199 ETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRSYATRKW 258
            T  KIP+G LF FV CP+Y  EI+ + GF+ ++Q L    F++  A  +   +    + 
Sbjct: 224 STERKIPRGLLFEFVSCPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRR 283

Query: 259 YISKF-ENFPNHVKALLPFV 266
           Y  +F + +P   K L+PF+
Sbjct: 284 YRKEFGDKYPKSRKILIPFL 299

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BLAST of PI0016022 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.0e-17
Identity = 41/107 (38.32%), Postives = 64/107 (59.81%), Query Frame = 0


Query: 160 IGVALFLIGSIGNFYHHYLLSQTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFI 219
           IG  L+L G + N +  ++L   RK G+T YKIP+GGLF +V   +Y  EI+ + G+A  
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212

Query: 220 SQELYPLCFSIGTAIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 267
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K ++PF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of PI0016022 vs. ExPASy Swiss-Prot
Match: O18765 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 4.0e-17
Identity = 43/108 (39.81%), Postives = 69/108 (63.89%), Query Frame = 0


Query: 159 NIGVALFLIGSIGNFYHHYLLSQTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAF 218
           ++GV LF++G   N +  Y+L Q RK GE  YKIP+GGLF++V   ++L EII + G+A 
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYAL 206

Query: 219 ISQELYPLCFSIGTAIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 267
            +  L  L F+  +  +L  R++   ++Y+  FE++P   KAL+PF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of PI0016022 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-16
Identity = 54/174 (31.03%), Postives = 88/174 (50.57%), Query Frame = 0


Query: 95  LLKSALTLHFFKRIFEVLFIHKYSSKM-VLETAIKISFSYFMSTAIIIYTQHLSLGLPEP 154
           +L +   +H+  R     F+ +    M +L   + I F  F       Y  H ++   + 
Sbjct: 90  ILLAMFLVHYGHRCLIYPFLMRGGKPMPLLACTMAIMFCTFNGYLQSRYLSHWAVYADDW 149

Query: 155 PVDLK-NIGVALFLIGSIGNFYHHYLLSQTRKQGETAYKIPKGGLFSFVVCPHYLFEIIG 214
             D +  IG  L+L G + N +  ++L   RK G+T YKIP+GGLF +V   +Y  EI+ 
Sbjct: 150 VTDPRFLIGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIME 209

Query: 215 YFGFAFISQELYPLCFSIGTAIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 267
           + G+A  S  +    F+  T  +L+GR+    +WY+ KFE +P   K L+PF+F
Sbjct: 210 WCGYALASWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of PI0016022 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-16
Identity = 42/105 (40.00%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 161 GVALFLIGSIGNFYHHYLLSQTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFIS 220
           G AL+L+G + N +  ++L   RK GET YKIP+GGLF +V   +Y  E++ + GFA  S
Sbjct: 150 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFGELVEWCGFALAS 209

Query: 221 QELYPLCFSIGTAIYLAGRSYATRKWYISKFENFPNHVKALLPFV 266
             L  + F++ T   L  R+    +WY+ KFE++P   K L+PF+
Sbjct: 210 WSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKTRKILIPFL 254

[Close]

BLAST of PI0016022 vs. ExPASy TrEMBL
Match: A0A1S3BFS0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.1e-138
Identity = 249/266 (93.61%), Postives = 257/266 (96.62%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           MD+LSAFLFPPPPSTFVNVMTVVSSTSLAMAGL EI GKHLQYSKFWNANSG KLSA+ES
Sbjct: 11  MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLL YTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 71  KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 130

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MVL+TAI+ISFSYF+STAI+IYTQHLS GLPEPP+DLKNIGV LFL G IGNFYHHYLLS
Sbjct: 131 MVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHYLLS 190

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           QTRKQ ETAYKIPKGGLFSFV+CPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS
Sbjct: 191 QTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 250

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWYISKFENFPNHVKALLPFVF
Sbjct: 251 YATRKWYISKFENFPNHVKALLPFVF 276

[Close]

BLAST of PI0016022 vs. ExPASy TrEMBL
Match: A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 8.4e-127
Identity = 225/266 (84.59%), Postives = 245/266 (92.11%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           M +LSAFLFPPPPSTFVNVMTVVSST++ MAG+ EINGKHLQYSKFWNANS PK  ++E 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFW FPS +ER  +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MV +TAIKISFSY +ST I+IYTQHLS GLPEPP+DLKNIG+ALFLIG IGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           +TRK GET YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of PI0016022 vs. ExPASy TrEMBL
Match: A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.4e-126
Identity = 224/266 (84.21%), Postives = 245/266 (92.11%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           M +LSAFLFPPPPSTFVNVMTVVSST++ MAG+ EINGKHLQYSKFWNANS PK  ++E 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFW FPS +ER  +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MV +TAIKISFSY +ST I+IYTQHLS G+PEPP+DLKNIG+ALFLIG IGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           +TRK GET YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of PI0016022 vs. ExPASy TrEMBL
Match: A0A0A0KTC7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612880 PE=3 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.3e-122
Identity = 227/266 (85.34%), Postives = 238/266 (89.47%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           MDVLSAFLFPPPPSTFVNVMTVVS+T+LAMAGL EINGKHLQYSKFWNAN   KLSA+ES
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFWLFPSGDER                  VLFIHKYSSK
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDER------------------VLFIHKYSSK 140

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MVL+TAI+IS SYFMSTAI+IYTQHLS GLPEPP+DLKNIGV  FLIG IGNFYHHYLLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           QTRKQG+TAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTA+YLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWYISKFE FPN+VKALLPFVF
Sbjct: 261 YATRKWYISKFEAFPNNVKALLPFVF 268

[Close]

BLAST of PI0016022 vs. ExPASy TrEMBL
Match: A0A6J1DAI6 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111018692 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.9e-118
Identity = 209/266 (78.57%), Postives = 235/266 (88.35%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           MD+ SAFLFPPPPSTFVNVMTVVSST++AM+G  E+NGKHLQYSKFWNAN   K S++ES
Sbjct: 1   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFW FPS D R  LLKSALT HFFKRIFEVLF+HKYSSK
Sbjct: 61  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 120

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MVL+TA KIS SYF+STAI+IYTQHLS G P P +DLKNIG+  F++G IGNFYHHYLLS
Sbjct: 121 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 180

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           +TR +GE  YKIP GGLF  V+CPHYLFEII YFGFAFISQ ++PLCF+ GTA+YLAGRS
Sbjct: 181 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFE+FPNHVKALLPF+F
Sbjct: 241 YATRKWYVSKFEHFPNHVKALLPFLF 266

[Close]

BLAST of PI0016022 vs. NCBI nr
Match: XP_008446722.1 (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo])

HSP 1 Score: 501.5 bits (1290), Expect = 4.4e-138
Identity = 249/266 (93.61%), Postives = 257/266 (96.62%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           MD+LSAFLFPPPPSTFVNVMTVVSSTSLAMAGL EI GKHLQYSKFWNANSG KLSA+ES
Sbjct: 11  MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSAQES 70

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLL YTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 71  KLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 130

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MVL+TAI+ISFSYF+STAI+IYTQHLS GLPEPP+DLKNIGV LFL G IGNFYHHYLLS
Sbjct: 131 MVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHYLLS 190

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           QTRKQ ETAYKIPKGGLFSFV+CPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS
Sbjct: 191 QTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 250

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWYISKFENFPNHVKALLPFVF
Sbjct: 251 YATRKWYISKFENFPNHVKALLPFVF 276

[Close]

BLAST of PI0016022 vs. NCBI nr
Match: XP_004135052.2 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothetical protein Csa_008329 [Cucumis sativus])

HSP 1 Score: 490.3 bits (1261), Expect = 1.0e-134
Identity = 244/266 (91.73%), Postives = 255/266 (95.86%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           MDVLSAFLFPPPPSTFVNVMTVVS+T+LAMAGL EINGKHLQYSKFWNAN   KLSA+ES
Sbjct: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
           K+SSKYGMLLAYTPAFLAGAASFWLFPSGDER FLLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 140

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MVL+TAI+IS SYFMSTAI+IYTQHLS GLPEPP+DLKNIGV  FLIG IGNFYHHYLLS
Sbjct: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           QTRKQG+TAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTA+YLAGRS
Sbjct: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWYISKFE FPN+VKALLPFVF
Sbjct: 261 YATRKWYISKFEAFPNNVKALLPFVF 286

[Close]

BLAST of PI0016022 vs. NCBI nr
Match: XP_038891781.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 476.1 bits (1224), Expect = 2.0e-130
Identity = 233/266 (87.59%), Postives = 252/266 (94.74%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           MDVLSAFLFPPPPS FVN+MTVVSST+LAMAG+ EINGKHLQYSKFWNAN+ PKLS++ S
Sbjct: 34  MDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSKFWNANTAPKLSSEVS 93

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
            LSSKYGMLLAYTPAFLAGAASFWLFPS DER  LLKSALTLHFFKRIFEVLFIHKYSSK
Sbjct: 94  MLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFFKRIFEVLFIHKYSSK 153

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MVL TAI+ISFSYF+ST I+IYTQHLS GLPEPP++LKNIG+ LFLIG IGNFYHHYLLS
Sbjct: 154 MVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLFLIGIIGNFYHHYLLS 213

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           +TRK+GET+Y+IPKGGLFSFV+CPHYLFEII +FGFAFISQELYPLCFSIGT+IYLAGRS
Sbjct: 214 KTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYPLCFSIGTSIYLAGRS 273

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWYISKFENFPNHVKALLPFVF
Sbjct: 274 YATRKWYISKFENFPNHVKALLPFVF 299

[Close]

BLAST of PI0016022 vs. NCBI nr
Match: XP_022957108.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 463.0 bits (1190), Expect = 1.7e-126
Identity = 225/266 (84.59%), Postives = 245/266 (92.11%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           M +LSAFLFPPPPSTFVNVMTVVSST++ MAG+ EINGKHLQYSKFWNANS PK  ++E 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFW FPS +ER  +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MV +TAIKISFSY +ST I+IYTQHLS GLPEPP+DLKNIG+ALFLIG IGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           +TRK GET YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of PI0016022 vs. NCBI nr
Match: XP_022984951.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])

HSP 1 Score: 462.2 bits (1188), Expect = 3.0e-126
Identity = 224/266 (84.21%), Postives = 245/266 (92.11%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKES 60
           M +LSAFLFPPPPSTFVNVMTVVSST++ MAG+ EINGKHLQYSKFWNANS PK  ++E 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 61  KLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSSK 120
           KLSSKYGMLLAYTPAFLAGAASFW FPS +ER  +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 121 MVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLLS 180
           MV +TAIKISFSY +ST I+IYTQHLS G+PEPP+DLKNIG+ALFLIG IGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 181 QTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAGRS 240
           +TRK GET YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTAIYLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 241 YATRKWYISKFENFPNHVKALLPFVF 267
           YATRKWY+SKFENFP+HVKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of PI0016022 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 312.4 bits (799), Expect = 3.5e-85
Identity = 151/268 (56.34%), Postives = 205/268 (76.49%), Query Frame = 0


Query: 1   MDVLSAFLFPPPPSTFVNVMTVVSSTSLAMAGLFEINGKHLQYSKFWNANSGPKLSAKE- 60
           M+++++F++PPPPS  +N M+VV   +LA  G  EI G HL+YSKF  ++S P+   +  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 61  SKLSSKYGMLLAYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYSS 120
             +SS+ GMLL YTPAFLA A+SF++ PS D RF LLKSAL LHFFKR+FEVLFIHKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 121 KMVLETAIKISFSYFMSTAIIIYTQHLSLGLPEPPVDLKNIGVALFLIGSIGNFYHHYLL 180
            M +++A+ IS SYF STA+++Y+Q+L+LGL EP  D+K  GV +F++G +GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 181 SQTRKQ-GETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAG 240
           ++ RK+ G+  YKIPKGGLF  ++CPHYLFEI+ ++ F  ISQ +Y   F++GT +YL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 241 RSYATRKWYISKFENFPNHVKALLPFVF 267
           RSYATR WY+SKF++FP H+KAL+PFVF
Sbjct: 241 RSYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of PI0016022 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 72.8 bits (177), Expect = 4.7e-13
Identity = 39/108 (36.11%), Postives = 60/108 (55.56%), Query Frame = 0


Query: 160 IGVALFLIGSIGNFYHHYLLSQTRKQGETAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFI 219
           IG+ +F+ G   N      L + +K+    Y IP+GG F  V CP+Y  E I + G+A +
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 220 SQELYPLCFSIGTAIYLAGRSYATRKWYISKF-ENFPNHVKALLPFVF 267
           +     + F + T   L  R+ A+ KWYI+KF E +P   KA++PFV+
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of PI0016022 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 42/115 (36.52%), Postives = 56/115 (48.70%), Query Frame = 0


Query: 157 LKNIGVALFLIGSIGNFYHHYLLSQTRKQGETA--YKIPKGGLFSFVVCPHYLFEIIGYF 216
           L+ IG A+FL G I     H +L   R+    A  Y IP G  F  V CPH+L EI+ Y 
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266

Query: 217 GFAFISQELYPLCFSIGTAIYLAGRSYA---TRKWYISKFENFPNHVKALLPFVF 267
           G   IS     +   +      A  +YA   T +WY+ KFEN+P    A+ P V+
Sbjct: 267 GL-LISSGGTDISIWLLFGFVAANLTYAAGETHRWYLQKFENYPASRHAIFPHVY 320

[Close]

BLAST of PI0016022 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-08
Identity = 42/117 (35.90%), Postives = 57/117 (48.72%), Query Frame = 0


Query: 157 LKNIGVALFLIGSIGNFYHHYLLSQTRKQGETA--YKIPKGGLFSFVVCPHYLFEIIGYF 216
           L+ IG A+FL G I     H +L   R+    A  Y IP G  F  V  PH+L EI+ Y 
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289

Query: 217 GFAFISQ----ELYPLC-FSIGTAIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 267
           G    S      ++ L  F      Y AG    T +WY+ KFEN+P +  A+ P+V+
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343

[Close]

BLAST of PI0016022 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-08
Identity = 42/117 (35.90%), Postives = 57/117 (48.72%), Query Frame = 0


Query: 157 LKNIGVALFLIGSIGNFYHHYLLSQTRKQGETA--YKIPKGGLFSFVVCPHYLFEIIGYF 216
           L+ IG A+FL G I     H +L   R+    A  Y IP G  F  V  PH+L EI+ Y 
Sbjct: 229 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 288

Query: 217 GFAFISQ----ELYPLC-FSIGTAIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 267
           G    S      ++ L  F      Y AG    T +WY+ KFEN+P +  A+ P+V+
Sbjct: 289 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 342

The following BLAST results are available for this feature:

BLAST of PI0016022 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q55C17	2.4e-17	29.62	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]
P18405	4.0e-17	38.32	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
O18765	4.0e-17	39.81	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=...	[more]
Q28891	1.2e-16	31.03	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
Q68FF9	1.2e-16	40.00	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]

BLAST of PI0016022 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BFS0	2.1e-138	93.61	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A6J1GZA3	8.4e-127	84.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1J6R3	1.4e-126	84.21	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A0A0KTC7	1.3e-122	85.34	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128...	[more]
A0A6J1DAI6	1.9e-118	78.57	very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]

BLAST of PI0016022 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446722.1	4.4e-138	93.61	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo]	[more]
XP_004135052.2	1.0e-134	91.73	3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothet...	[more]
XP_038891781.1	2.0e-130	87.59	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida]	[more]
XP_022957108.1	1.7e-126	84.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]
XP_022984951.1	3.0e-126	84.21	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima]	[more]

BLAST of PI0016022 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	3.5e-85	56.34	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	4.7e-13	36.11	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	1.9e-09	36.52	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	1.2e-08	35.90	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.2	1.2e-08	35.90	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 92..265 e-value: 1.4E-6 score: 30.1
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 6..266
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 158..266 e-value: 1.5E-28 score: 99.6
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 161..241 score: 26.012838
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 6..266

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0016022.1	PI0016022.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors