PI0015515 (gene) Melon (PI 482460) v1

Overview
NamePI0015515
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionAvrRpt-cleavage domain-containing protein
Locationchr06: 20249988 .. 20251555 (+)
RNA-Seq ExpressionPI0015515
SyntenyPI0015515
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAAGAAAGAATTGTGGAAGTGATGTAAAGAGAAGTTAAGAAAGGAGCTTAAAATGGAGTTACCAAAATACTCGGCTTCTGTTATTCCCATTTTAGCTTCCAACACCCCATTCTCCCTCTTTCCTTCCCTCTTCCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCACTTTCATTTTCTGTGCAGAAGGCTTTGTTAATGTATTTTAATGGCTTCGGTAAGATTCTCTCTTCTTCTTCTATTCTAGATTCTCTCTTCTCCTTCTTCTATTCTATTTGAAATGCTATCGAATTTGATCCTTTCTGAAGATTTTGCAAAGTTCTTTGGGGAATGTGAATTGTGATAACGTAACATGTCTTAAAAGGAAAAACAAATCTATGTTATGGCTTAGCTATGGCGTTTTTATATTAGAAACGACATTTGCTTGTATCGAGCATGCATTTTCTCATTCTACCAATCGAATTACGGATATAAATCTATAATATGGCTTAGCTATGATGTTTTGTATTATTTTCTGGTAGAAAATAGAATTTTTGAATTTGTTGCCTATAAAACATTCGAAATGGGAAAGAATCTGGCATATATTTGGAATCTATTGTTTTGTTTTTCCTCTGTTGTGGGAACGATGATCATCATGAATTAGCTTCATTTACTTCTCCCTGACATACCTGCCTTGTTTGATACCCACATCAGCAGAATTTATTTCATTGCTTTCATAGAAATGAATGGCCAAAAGCAAGCACAAGATAGGGAGAGATCTACACATTAACATGGAACATTCTATGCAAGAGAAAAAGCAATGGAAGAACTGACTAATATGGCAATCACTAGTATAATTATGAATGAAAAAGCTCTGAGTTCTTGTACTTTTCTAATGTCTATAGCAGGATAAAGGTCGCCCCTTGCCGAAGTTCGGCGAGTGGGATGTCAACAACCCGGCATCCGCCGAAGGATTTACAGTAATATTTAGCAAAGCTAGAGATGAGAAGAAGTCGAATGAGGCTCCTGGGAATACAGCAGCAAAGGACAATACATCCAGACAAGAAGAGAATTATCAATATTCTCCCATGGTAAGTAATTTCACTAAGTTTACTTAGTCGGCCTGTGCACAGAAAACGCTAAGCAACTTATTAAAACATTATATTATATCATTGACTAAAACATCATAAAATGCTGGCTCGATGATCAAAATATCCATAAACTGAAGGGTTCGTCATCAATGTTAAATATCCATTGATATCTCAAATATTTTCTATAAATGTTCATAAAACATGAAAAATTTCATCTACTTAGTTCAATACGAATATGAATACCAACAACAATATCCATAATTGGGTTTGGAAGTAAAAACTTAATTATGAATCAAATAAACTTTATAATCTTTTTAATATTTACAAGAATATCCATCGATATCAATATCTTTTCGATATATCCATAAAATAGAAATCTCAATCTTGATTCCGACATTAATCCTAGACTCAACTTCGGCTTCATCTTCTTGCAATCTGGTTAGAATTAACTACAAATTTTTGTGCTTGGATGCAGAAAAAATGGTTTTGCTGTTTCTGA

mRNA sequence

AATAAAGAAAGAATTGTGGAAGTGATGTAAAGAGAAGTTAAGAAAGGAGCTTAAAATGGAGTTACCAAAATACTCGGCTTCTGTTATTCCCATTTTAGCTTCCAACACCCCATTCTCCCTCTTTCCTTCCCTCTTCCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCACTTTCATTTTCTGTGCAGAAGGCTTTGTTAATGTATTTTAATGGCTTCGGATAAAGGTCGCCCCTTGCCGAAGTTCGGCGAGTGGGATGTCAACAACCCGGCATCCGCCGAAGGATTTACAGTAATATTTAGCAAAGCTAGAGATGAGAAGAAGTCGAATGAGGCTCCTGGGAATACAGCAGCAAAGGACAATACATCCAGACAAGAAGAGAATTATCAATATTCTCCCATGAAAAAATGGTTTTGCTGTTTCTGA

Coding sequence (CDS)

ATGGCTTCGGATAAAGGTCGCCCCTTGCCGAAGTTCGGCGAGTGGGATGTCAACAACCCGGCATCCGCCGAAGGATTTACAGTAATATTTAGCAAAGCTAGAGATGAGAAGAAGTCGAATGAGGCTCCTGGGAATACAGCAGCAAAGGACAATACATCCAGACAAGAAGAGAATTATCAATATTCTCCCATGAAAAAATGGTTTTGCTGTTTCTGA

Protein sequence

MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQYSPMKKWFCCF
Homology
BLAST of PI0015515 vs. ExPASy Swiss-Prot
Match: O22633 (Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.2e-15
Identity = 41/70 (58.57%), Postives = 50/70 (71.43%), Query Frame = 0

Query: 3  SDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN--TAAKDNTSRQEENYQ 62
          SDKGRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+   +++ +      +  
Sbjct: 2  SDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGKSSEGHVKSGGGDPS 61

Query: 63 YSPMKKWFCC 71
              KKW CC
Sbjct: 62 KPQPKKWLCC 71

BLAST of PI0015515 vs. ExPASy Swiss-Prot
Match: Q8GYN5 (RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.1e-05
Identity = 27/69 (39.13%), Postives = 40/69 (57.97%), Query Frame = 0

Query: 4   DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSN-EAPGNTAAKDNTSRQEENYQYS 63
           +K   +PKFG+WD NNP+SA+G+T IF+K R+E+ S     G++    + S +  N   S
Sbjct: 143 EKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSGANVSGSSRTPTHQSSRNPNNTSS 202

Query: 64  PMKKWFCCF 72
                 CCF
Sbjct: 203 -----CCCF 206

BLAST of PI0015515 vs. ExPASy TrEMBL
Match: A0A0A0LDP9 (AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G595220 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 6.5e-34
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60
          MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ
Sbjct: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60

Query: 61 YSPMKKWFCCF 72
          YSPMKKWFCCF
Sbjct: 61 YSPMKKWFCCF 71

BLAST of PI0015515 vs. ExPASy TrEMBL
Match: A0A5D3E371 (RPM1-interacting protein 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold595G00620 PE=4 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-26
Identity = 61/63 (96.83%), Postives = 62/63 (98.41%), Query Frame = 0

Query: 2  ASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY 61
          + DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY
Sbjct: 19 SQDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY 78

Query: 62 SPM 65
          SPM
Sbjct: 79 SPM 81

BLAST of PI0015515 vs. ExPASy TrEMBL
Match: A0A5J5AK52 (AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_004861 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.3e-22
Identity = 56/74 (75.68%), Postives = 63/74 (85.14%), Query Frame = 0

Query: 2  ASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEA--PGNTAAK-DNTSRQEEN 61
          + DKGRPLPKFGEWDVNNPASA+GFTVIF+KARD+KK+N A  PG  + K DNTS+Q EN
Sbjct: 3  SQDKGRPLPKFGEWDVNNPASADGFTVIFAKARDDKKTNGAAGPGGPSQKNDNTSKQNEN 62

Query: 62 YQYSPM-KKWFCCF 72
          YQY PM KKWFCCF
Sbjct: 63 YQYPPMEKKWFCCF 76

BLAST of PI0015515 vs. ExPASy TrEMBL
Match: A0A5J5BYY8 (AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_004561 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.1e-20
Identity = 53/78 (67.95%), Postives = 61/78 (78.21%), Query Frame = 0

Query: 2  ASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEA-------PGNTAAKDNTSR 61
          + DKGRPLPKFGEWDVNNPASA+GFTVIF+KARD+KK+N A        G    K++ S+
Sbjct: 3  SQDKGRPLPKFGEWDVNNPASADGFTVIFAKARDDKKTNGAGAGAGGGGGGPTQKNDNSK 62

Query: 62 QEENYQYSPM-KKWFCCF 72
          Q ENYQYSPM KKWFCCF
Sbjct: 63 QNENYQYSPMEKKWFCCF 80

BLAST of PI0015515 vs. ExPASy TrEMBL
Match: A0A2P5B5J0 (RIN4, pathogenic type III effector avirulence factor Avr cleavage site OS=Parasponia andersonii OX=3476 GN=PanWU01x14_269930 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.4e-20
Identity = 54/76 (71.05%), Postives = 57/76 (75.00%), Query Frame = 0

Query: 2  ASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN---TAAKDNTS---RQ 61
          + DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSN A G+       DN S   + 
Sbjct: 3  SQDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNGAAGSGTTNQKNDNNSANNKP 62

Query: 62 EENYQYSPMKKWFCCF 72
           E Y Y PMKKWFCCF
Sbjct: 63 GETYDYPPMKKWFCCF 78

BLAST of PI0015515 vs. NCBI nr
Match: KGN58221.1 (hypothetical protein Csa_017629 [Cucumis sativus])

HSP 1 Score: 152.5 bits (384), Expect = 1.3e-33
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60
          MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ
Sbjct: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQ 60

Query: 61 YSPMKKWFCCF 72
          YSPMKKWFCCF
Sbjct: 61 YSPMKKWFCCF 71

BLAST of PI0015515 vs. NCBI nr
Match: XP_038883812.1 (protein NOI4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 141.4 bits (355), Expect = 3.1e-30
Identity = 65/68 (95.59%), Postives = 66/68 (97.06%), Query Frame = 0

Query: 4  DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQYSP 63
          DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNT AK+NTSR EENYQYSP
Sbjct: 30 DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTGAKNNTSRPEENYQYSP 89

Query: 64 MKKWFCCF 72
          MKKWFCCF
Sbjct: 90 MKKWFCCF 97

BLAST of PI0015515 vs. NCBI nr
Match: XP_038883820.1 (protein NOI4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 141.4 bits (355), Expect = 3.1e-30
Identity = 65/68 (95.59%), Postives = 66/68 (97.06%), Query Frame = 0

Query: 4  DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQYSP 63
          DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNT AK+NTSR EENYQYSP
Sbjct: 12 DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTGAKNNTSRPEENYQYSP 71

Query: 64 MKKWFCCF 72
          MKKWFCCF
Sbjct: 72 MKKWFCCF 79

BLAST of PI0015515 vs. NCBI nr
Match: KAG6581070.1 (Protein NOI4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 139.8 bits (351), Expect = 9.0e-30
Identity = 64/68 (94.12%), Postives = 66/68 (97.06%), Query Frame = 0

Query: 4  DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQYSP 63
          D+GRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAK+N SR EENYQYSP
Sbjct: 27 DRGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKNNASRPEENYQYSP 86

Query: 64 MKKWFCCF 72
          MKKWFCCF
Sbjct: 87 MKKWFCCF 94

BLAST of PI0015515 vs. NCBI nr
Match: KAA0066959.1 (RPM1-interacting protein 4-like [Cucumis melo var. makuwa] >TYK30238.1 RPM1-interacting protein 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 128.3 bits (321), Expect = 2.7e-26
Identity = 61/63 (96.83%), Postives = 62/63 (98.41%), Query Frame = 0

Query: 2  ASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY 61
          + DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY
Sbjct: 19 SQDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQY 78

Query: 62 SPM 65
          SPM
Sbjct: 79 SPM 81

BLAST of PI0015515 vs. TAIR 10
Match: AT5G40645.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 83.2 bits (204), Expect = 9.3e-17
Identity = 44/67 (65.67%), Postives = 51/67 (76.12%), Query Frame = 0

Query: 7  RPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAA--KDNTSRQEENYQYSPM 66
          RPLPKFGEWDVN+PASAEGFTVIF+KARD+KK+N A G  A+  +DN   Q+E     P 
Sbjct: 13 RPLPKFGEWDVNDPASAEGFTVIFAKARDDKKTN-ASGRAASQRRDNNKSQDE-----PT 72

Query: 67 KKWFCCF 72
          KK FCCF
Sbjct: 73 KKRFCCF 73

BLAST of PI0015515 vs. TAIR 10
Match: AT5G63270.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 82.8 bits (203), Expect = 1.2e-16
Identity = 42/78 (53.85%), Postives = 51/78 (65.38%), Query Frame = 0

Query: 1  MASDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEA----PGNTAAKDNTSRQE 60
          MA +KG+PLPKFGEWDVNNPASAEGFTVIFSKA DEKK+ +A    P +  +       +
Sbjct: 1  MAENKGKPLPKFGEWDVNNPASAEGFTVIFSKASDEKKTKKASGAGPNSLVSPQRNQNSD 60

Query: 61 ENYQYSPM-----KKWFC 70
          +N  +S        KWFC
Sbjct: 61 QNNNHSSQNPKAKNKWFC 78

BLAST of PI0015515 vs. TAIR 10
Match: AT2G04410.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 81.6 bits (200), Expect = 2.7e-16
Identity = 38/68 (55.88%), Postives = 46/68 (67.65%), Query Frame = 0

Query: 3  SDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGNTAAKDNTSRQEENYQYS 62
          +DKGRPLPKFGEWDVN+P+SAEGFTVIF+KAR+EKK      +    +    +       
Sbjct: 2  ADKGRPLPKFGEWDVNDPSSAEGFTVIFNKARNEKKGGGKSDSPGKDEPGYNKNGEVLEK 61

Query: 63 PMKKWFCC 71
          P KKWFCC
Sbjct: 62 PAKKWFCC 69

BLAST of PI0015515 vs. TAIR 10
Match: AT5G55850.3 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 80.9 bits (198), Expect = 4.6e-16
Identity = 40/69 (57.97%), Postives = 49/69 (71.01%), Query Frame = 0

Query: 4  DKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN--TAAKDNTSRQEENYQY 63
          DKGRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+   +++ +      +   
Sbjct: 19 DKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGSPGKSSEGHVKSGGGDPSK 78

Query: 64 SPMKKWFCC 71
             KKW CC
Sbjct: 79 PQPKKWLCC 87

BLAST of PI0015515 vs. TAIR 10
Match: AT5G55850.1 (RPM1-interacting protein 4 (RIN4) family protein )

HSP 1 Score: 79.0 bits (193), Expect = 1.8e-15
Identity = 36/43 (83.72%), Postives = 40/43 (93.02%), Query Frame = 0

Query: 3  SDKGRPLPKFGEWDVNNPASAEGFTVIFSKARDEKKSNEAPGN 46
          SDKGRPLPKFGEWDVN+PASAEGFTVIF+KARDEKK+   PG+
Sbjct: 2  SDKGRPLPKFGEWDVNDPASAEGFTVIFNKARDEKKTGGKPGS 44

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O226332.2e-1558.57Protein NOI4 OS=Arabidopsis thaliana OX=3702 GN=NOI4 PE=1 SV=1[more]
Q8GYN52.1e-0539.13RPM1-interacting protein 4 OS=Arabidopsis thaliana OX=3702 GN=RIN4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LDP96.5e-34100.00AvrRpt-cleavage domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G59... [more]
A0A5D3E3711.3e-2696.83RPM1-interacting protein 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A5J5AK523.3e-2275.68AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_0... [more]
A0A5J5BYY81.1e-2067.95AvrRpt-cleavage domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_0... [more]
A0A2P5B5J02.4e-2071.05RIN4, pathogenic type III effector avirulence factor Avr cleavage site OS=Parasp... [more]
Match NameE-valueIdentityDescription
KGN58221.11.3e-33100.00hypothetical protein Csa_017629 [Cucumis sativus][more]
XP_038883812.13.1e-3095.59protein NOI4-like isoform X1 [Benincasa hispida][more]
XP_038883820.13.1e-3095.59protein NOI4-like isoform X2 [Benincasa hispida][more]
KAG6581070.19.0e-3094.12Protein NOI4, partial [Cucurbita argyrosperma subsp. sororia][more]
KAA0066959.12.7e-2696.83RPM1-interacting protein 4-like [Cucumis melo var. makuwa] >TYK30238.1 RPM1-inte... [more]
Match NameE-valueIdentityDescription
AT5G40645.19.3e-1765.67RPM1-interacting protein 4 (RIN4) family protein [more]
AT5G63270.11.2e-1653.85RPM1-interacting protein 4 (RIN4) family protein [more]
AT2G04410.12.7e-1655.88RPM1-interacting protein 4 (RIN4) family protein [more]
AT5G55850.34.6e-1657.97RPM1-interacting protein 4 (RIN4) family protein [more]
AT5G55850.11.8e-1583.72RPM1-interacting protein 4 (RIN4) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008700RIN4, pathogenic type III effector avirulence factor Avr cleavage sitePFAMPF05627AvrRpt-cleavagecoord: 4..39
e-value: 2.4E-23
score: 81.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 44..59
NoneNo IPR availablePANTHERPTHR33159:SF69RPM1-INTERACTING PROTEIN 4-LIKEcoord: 1..71
IPR040387RPM1-interacting protein 4/NOI4PANTHERPTHR33159RPM1-INTERACTING PROTEIN 4 (RIN4) FAMILY PROTEINcoord: 1..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0015515.1PI0015515.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005886 plasma membrane