PI0015183 (gene) Melon (PI 482460) v1

BLAST of PI0015183 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.1e-140
Identity = 249/528 (47.16%), Postives = 355/528 (67.23%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPT 60
           + L  I  +S   L+FL  F+         +  R +  P  SG+ P++GHL  L+ ++  
Sbjct: 8   LNLNTIAIASILSLIFLCLFL-------YRKNSRGKDAPVVSGAWPILGHLSLLNGSQTP 67

Query: 61  HKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNY 120
           HK L  +AD YGP+FT++LGM  ALV+S+WE+++E FTTND   +SRPK +A E++ YN 
Sbjct: 68  HKVLGALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQ 127

Query: 121 AAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNK- 180
           A +G++PYGPYWR +RKI T E L+N R+EQ  +IR+SEV+TSIK+L+++  +   N   
Sbjct: 128 AFVGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESR 187

Query: 181 -ATVEMKTWFEKLTMNTILRLVVGKRFDSAF--QGID-GEKYRKALRDLIYLFGAFVPSD 240
              V++K W   LT N ++R+VVGKR+      +G D  +++ K +R+ + L G F  +D
Sbjct: 188 YTLVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVAD 247

Query: 241 SFPFLSWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKNEMERNEEDFLDVMLSIVE 300
             P L WLD+GG++KAMK  AK +D++  +W+EE R+ K   E   ++ DF+DVM+S + 
Sbjct: 248 GVPCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISAL- 307

Query: 301 HHGEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGR 360
           +  +   FD DT+ KAT L +ILGG D+T +T+TWALSLLL N  AL KA+ E+D ++G+
Sbjct: 308 NGAQIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGK 367

Query: 361 QRRVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQK 420
              +  SD++ L YLQAIVKETLRLYPP P   P E TE+CI+ GYHI   TRLI N+ K
Sbjct: 368 DEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWK 427

Query: 421 LQQEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLA 480
           + ++P +W DP EF+PERFLT+HKD DL+G NF+L+PFGSGRR+C G+S  L ++H TLA
Sbjct: 428 IHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLA 487

Query: 481 NLLHEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQCM 524
           NLLH F+I  PS E ++M E FG T  K TPL+++V PR     Y+ +
Sbjct: 488 NLLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPRQSPNYYETL 527

BLAST of PI0015183 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 5.7e-140
Identity = 258/523 (49.33%), Postives = 355/523 (67.88%), Query Frame = 0

Query: 3   LPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPTHK 62
           L  +L   F LL   + FV       I +      PP+ +G+ P+IGHLH L+  +  HK
Sbjct: 7   LETLLLGFFVLLPCFFYFVWKKPNNKIKE------PPQPAGAWPIIGHLHLLARGDLPHK 66

Query: 63  TLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNYAA 122
            L+  AD  GP+F ++LG++ ALVV++ EIA+ECFTTNDR F +RP  +A++++GYNY  
Sbjct: 67  ILSSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVM 126

Query: 123 MGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKATV 182
           +GV+PYGPYWR +RKI  LE L+N RL+  +++  SE+  S K+LY+L  +++N +   V
Sbjct: 127 LGVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKL-WETQNIDFCLV 186

Query: 183 EMKTWFEKLTMNTILRLVVGKRF-----DSAFQGIDGEKYRKALRDLIYLFGAFVPSDSF 242
           +MK W   LT+N  +++VVGKRF      SA +  +     K LR++  L G+FV SD  
Sbjct: 187 DMKQWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYL 246

Query: 243 PFLSWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKNEMERNEEDFLDVMLSIVEHH 302
           P+L WLD+GG++K MK T K LD +F  W++E +  +     E +++DF+DVMLSI+E  
Sbjct: 247 PYLRWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEE- 306

Query: 303 GEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQR 362
               G DVDT+ K  CL +ILGGADTT  T+TWALSLLLNN  ALKKAQ ELD  VGR R
Sbjct: 307 -SKLGNDVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDR 366

Query: 363 RVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQ 422
            V+ SD+  LTY+ AI+KETLRLYPP PLL P   TEDC ++GYH+ A TRLIVN  K+Q
Sbjct: 367 NVDESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQ 426

Query: 423 QEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANL 482
           ++PL+W  P E++PERFL   +D D+KGQ+F+LIPFGSGRR CP IS ALQ++ LTLA++
Sbjct: 427 RDPLVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHI 486

Query: 483 LHEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVY 521
           LH FE+  P+   ++M E+ G+   K TPL+V+V PR+  K +
Sbjct: 487 LHGFELRTPNQNKVDMTETPGIVHAKATPLEVLVAPRISPKCF 518

BLAST of PI0015183 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 486.5 bits (1251), Expect = 3.8e-136
Identity = 253/543 (46.59%), Postives = 348/543 (64.09%), Query Frame = 0

Query: 9   SSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPTHKTLAKMA 68
           S  +LL FL+ F +++  +          PP  SGS P++GHL  +  T+  HKTL  + 
Sbjct: 15  SLISLLFFLFRFSKVSHTKE---------PPIISGSWPLLGHLPLMRNTQTPHKTLGALV 74

Query: 69  DVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNYAAMGVSPY 128
           D YGPIFT++LG   ALV+S+WE+A+ECFT ND + +SRPK +A EL+ YN A +G +PY
Sbjct: 75  DKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPY 134

Query: 129 GPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKL-----------YELCGDSRNN 188
           G YWR +RKI TLE+L+N R+E   +IR+SEVQTSIK+L           Y L  D++++
Sbjct: 135 GAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSS 194

Query: 189 NK---------ATVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKA------LR 248
           +           +VE+K WF +LT+N +LR+VVGKR    F  +D E   +A      +R
Sbjct: 195 STNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKR---CFGDVDVENKEEAKRFLENIR 254

Query: 249 DLIYLFGAFVPSDSFPFLSWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKNEMER- 308
           D + L G F   D  PFL WLD+GG++K MK  AK  D + ++W+EE RE K     ++ 
Sbjct: 255 DFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDKV 314

Query: 309 -NEEDFLDVMLSIVEHHGEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQE 368
             E DF+D ML +++      GFDVDT+ KAT L +ILGG+DTT  T+TWA+ LLL +  
Sbjct: 315 VGERDFMDAMLLVLKDK-PIEGFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPH 374

Query: 369 ALKKAQLELDEKVGRQRRVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSG 428
            L+K + EL+  +G++R V  SD+  L YL AI+KETLRLYPP P   P E TEDC + G
Sbjct: 375 VLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGG 434

Query: 429 YHIPAKTRLIVNVQKLQQEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMC 488
           YHI   TRL+ N+ K+ ++P +W DP EF+PERFL++HKD D++GQNF+L+PFGSGRRMC
Sbjct: 435 YHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMC 494

Query: 489 PGISFALQLMHLTLANLLHEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVY 524
            G+S  L ++H  LAN LH FEI  PS E +++ E       K TPL+V+V P L  K Y
Sbjct: 495 AGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCLSFKCY 544

BLAST of PI0015183 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 5.0e-136
Identity = 246/522 (47.13%), Postives = 355/522 (68.01%), Query Frame = 0

Query: 9   SSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEP-THKTLAKM 68
           S F+L + +  FV I   +  +++ +    P  SG+ P+IGHLH L   E   ++TL KM
Sbjct: 4   SLFSLFVPILVFVFIALFKK-SKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKM 63

Query: 69  ADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNYAAMGVSP 128
           AD YGP  +L+LG N A VVSS+E+A++CFT ND+  ASRP   A++ +GYN+A  G +P
Sbjct: 64  ADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAP 123

Query: 129 YGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKATVEMKTW 188
           Y  +WR +RKIAT+ELL+N RL+  +++R+SE+   +K LY L   +       V++K+W
Sbjct: 124 YSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSW 183

Query: 189 FEKLTMNTILRLVVGKRFDSAFQGIDGE------KYRKALRDLIYLFGAFVPSDSFPFLS 248
            E +T+N I+R+V GKR+      +  E      + +KA+    +L G F  SD+FP LS
Sbjct: 184 LEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLS 243

Query: 249 WLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKNEMERNEEDFLDVMLSIVEHHGEFP 308
           + D+ G++K MK T   LD + ++WIE  R+ +K +  + N+ DF+DVM+S+ E  G+  
Sbjct: 244 FFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAE-QGKLS 303

Query: 309 --GFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQRRV 368
              +D +T  K+TCL +ILGG+DT+  T+TWA+SLLLNN+E LKKAQ E+D  VGR R V
Sbjct: 304 HLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNV 363

Query: 369 EASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQQE 428
           E SD+ NL YLQAI+KETLRLYP  PLL P E+ EDC V+GY++P  TRLIVNV K+Q++
Sbjct: 364 EDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRD 423

Query: 429 PLIWEDPCEFRPERFLTSH-KDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANLL 488
           P ++ +P EFRPERF+T   K+FD++GQNF+L+PFGSGRR CPG S A+Q++HL LA  L
Sbjct: 424 PKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFL 483

Query: 489 HEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVY 521
           H F++    D  ++M E+ GLTI K TPL+V++ PR+  +++
Sbjct: 484 HSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523

BLAST of PI0015183 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 7.4e-132
Identity = 247/520 (47.50%), Postives = 344/520 (66.15%), Query Frame = 0

Query: 6   ILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPTHKTLA 65
           +++  F L LFLY  ++     S       +  P   G+ P+ GHL  L  ++  HK L 
Sbjct: 15  VVSLIFLLCLFLYGPLKKVHGSS-------KEAPTVGGAWPIFGHLPLLIGSKSPHKALG 74

Query: 66  KMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNYAAMGV 125
            +A+ +GP+FT++LG   ALVVS WE+ARECFTTND   ++RPK L +EL+ YN A + V
Sbjct: 75  ALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLV 134

Query: 126 SPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRN-NNKATVEM 185
           +PYGPYWR +RKI   E+L++ R+EQ + +R+SEVQ SI +LY++    +N ++ A+VE+
Sbjct: 135 APYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVEL 194

Query: 186 KTWFEKLTMNTILRLVVGKRFDSAFQGID-GEKYRKALRDLIYLFGAFVPSDSFPFLSWL 245
           K WF +   N +LR+VVGKRF SA    +  EK  KA+ + + L G F   D+ P+L WL
Sbjct: 195 KQWFAQPIFNMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWL 254

Query: 246 DVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKNEMERNEEDFLDVMLSIVEHHGEFPGF 305
           D GGY+KAMK TAK LD +  +W+EE R+ +   E     +DF++VMLS ++      G 
Sbjct: 255 DFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSLDGK-TIDGI 314

Query: 306 DVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQRRVEASD 365
           D DT+ K+T LT+I  G + ++ T+ WA+ L+L N   L+  + ELD +VG+ R +  SD
Sbjct: 315 DADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGKDRCICESD 374

Query: 366 VTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQQEPLIW 425
           ++NL YLQA+VKETLRLY P PL  P E  EDC + GYH+   TRLI N+ K+  +P +W
Sbjct: 375 ISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNVW 434

Query: 426 EDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANLLHEFEI 485
            DP EF+P+RFLT+HKD D+KG +FQL+PFGSGRR+CPGISF LQ +HL LA+ LH FEI
Sbjct: 435 SDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFEI 494

Query: 486 SRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQCM 524
             PS E L+M E+FG+T  K TPL+V+V P L    Y+ M
Sbjct: 495 LNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCYKSM 525

BLAST of PI0015183 vs. ExPASy TrEMBL
Match: A0A6J1FBD1 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442568 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 8.0e-222
Identity = 388/523 (74.19%), Postives = 440/523 (84.13%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPT 60
           MEL H+L + F LLLFLY    ITSRRS A +   RLPPEA G LPVIGHLHKLSATEPT
Sbjct: 1   MELTHVLPAIFALLLFLYALFAITSRRSSAHRTNKRLPPEAGGRLPVIGHLHKLSATEPT 60

Query: 61  HKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNY 120
           H TLAKMAD YGPIFTLRLGMNTALVVSSWEIARECFTTND IFASR KA+ASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDSIFASRSKAVASELLGYNY 120

Query: 121 AAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKA 180
             + +SPYGPYWRH+RKIATLELLTNHRL Q ++IR+SEV++SIKKLYEL  +  +  K 
Sbjct: 121 TVLSLSPYGPYWRHVRKIATLELLTNHRLHQLQHIRISEVRSSIKKLYEL--NKGSGGKV 180

Query: 181 TVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDSFPFL 240
            VEM+TWF   T+NTI R+V+GKRF + F+G  GE+YR+ALRDL+ L GAFVPSDSFPFL
Sbjct: 181 RVEMRTWFGDATLNTIFRMVMGKRFSTVFEGSGGEQYRQALRDLLELLGAFVPSDSFPFL 240

Query: 241 SWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKN--EMERNEEDFLDVMLSIVEHHG 300
           SWLD+GGYKKAM  TAKVLDQ+  KWI+E RE  K +    E  E+DF+D+MLS V   G
Sbjct: 241 SWLDLGGYKKAMNKTAKVLDQMLSKWIKEHREKTKTSGERGETEEQDFMDLMLSAVHDDG 300

Query: 301 EFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQRR 360
              GFD DTVTKATCLTMILGG+DTT +TMTWALSLLLNN++ALKKAQLELDEKVGR R 
Sbjct: 301 GLCGFDADTVTKATCLTMILGGSDTTTVTMTWALSLLLNNEQALKKAQLELDEKVGRSRH 360

Query: 361 VEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQQ 420
           V+ SDV NL YLQAIVKETLRLYP VPLL+PHES  DC+VSGYHIPAKTRLIVNVQKLQ+
Sbjct: 361 VKESDVKNLPYLQAIVKETLRLYPAVPLLVPHESMVDCMVSGYHIPAKTRLIVNVQKLQK 420

Query: 421 EPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANLL 480
           +PL+WEDP EFRPERFLTS   FD++GQN QL+PFGSGRRMCPGISFALQLMHLTLA+LL
Sbjct: 421 DPLVWEDPSEFRPERFLTSDTKFDMRGQNQQLMPFGSGRRMCPGISFALQLMHLTLASLL 480

Query: 481 HEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQ 522
           HEFEI RPS E+LNM+E FG+T+LKKTPL+VV+ PRLP +VY+
Sbjct: 481 HEFEIGRPSQELLNMDEGFGITVLKKTPLEVVLSPRLPIQVYE 521

BLAST of PI0015183 vs. ExPASy TrEMBL
Match: A0A6J1F723 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442948 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 3.2e-218
Identity = 388/523 (74.19%), Postives = 436/523 (83.37%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRN-RLPPEASGSLPVIGHLHKLSATEP 60
           MEL HIL ++F LLLFLY   RI SRRS A    N R PPEA+G+LP+IGHLHKLSATEP
Sbjct: 1   MELTHILPATFALLLFLYALFRIASRRSSAAHRSNKRHPPEAAGALPLIGHLHKLSATEP 60

Query: 61  THKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYN 120
           TH TLAKMAD YGPIFTLRLGMNTALVVSSWEIARECFTTND IFASR K +ASELLGYN
Sbjct: 61  THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRSKVVASELLGYN 120

Query: 121 YAAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRN--- 180
           YAA+G+SPYGPYWRH+RK+ATLELLTNHRLEQ ++IR+ EVQ SIKKLYELC DSRN   
Sbjct: 121 YAALGISPYGPYWRHVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGS 180

Query: 181 NNKATVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDS 240
             K  VEM+ WF  +++NTI R+VVGKRF + F+G   E+YR+ALR    LF AFVPSD 
Sbjct: 181 GGKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGSGAEQYREALRAFFELFAAFVPSDL 240

Query: 241 FPFLSWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKK---KNEMERNEEDFLDVMLSI 300
           FPFLSWLD+GGYKKAM  TAKVLDQ+  KWIEE RE KK   +  +E  E+DF+D+MLS 
Sbjct: 241 FPFLSWLDLGGYKKAMNKTAKVLDQMLSKWIEEHREKKKNWGEGGVETEEQDFMDLMLSA 300

Query: 301 VEHHGEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKV 360
           V   G   G   DTVTKATCLTMILGG DTT ITMTWALSLLLN+QEALKKAQLELDEK+
Sbjct: 301 VHDDGGLCGIGGDTVTKATCLTMILGGLDTTTITMTWALSLLLNDQEALKKAQLELDEKI 360

Query: 361 GRQRRVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNV 420
           GR R+V+ SDV NLTYLQAIVKETLRLYP  PLLIPHES EDCIVSGYHIPA+TRL+VNV
Sbjct: 361 GRSRQVKESDVKNLTYLQAIVKETLRLYPAAPLLIPHESVEDCIVSGYHIPARTRLLVNV 420

Query: 421 QKLQQEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLT 480
           QKLQ++PL+WEDPCEFRPERFL SH  FDL+GQN QL+PFGSGRR CPGISF+LQLMHLT
Sbjct: 421 QKLQKDPLVWEDPCEFRPERFLISHTKFDLRGQNHQLMPFGSGRRKCPGISFSLQLMHLT 480

Query: 481 LANLLHEFEISRPSDEMLNM-EESFGLTILKKTPLQVVVCPRL 516
           LANLLHEFEI RPS+++L+  EE FGLT +KKTPL+VV+ PRL
Sbjct: 481 LANLLHEFEIGRPSEKLLDKEEEGFGLTGVKKTPLEVVLSPRL 523

BLAST of PI0015183 vs. ExPASy TrEMBL
Match: A0A6J1F6F5 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442560 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.6e-217
Identity = 381/524 (72.71%), Postives = 436/524 (83.21%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPT 60
           MEL H + ++F LLLFLY   RI+SRRS A +  N LPPEA G LPVIGHLHKLSATEPT
Sbjct: 41  MELTHNIPATFALLLFLYALFRISSRRSAAHRTNNTLPPEAGGRLPVIGHLHKLSATEPT 100

Query: 61  HKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNY 120
           H TLAKMAD YGPIFTLRLGMNTALVVSSWEIARECFTTND IFASR K LASELLGYNY
Sbjct: 101 HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRSKGLASELLGYNY 160

Query: 121 AAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKA 180
             + VSPYGPYWRH+RKIAT ELL N RLEQ ++IR+SEV++SIKKLYEL  +  +  K 
Sbjct: 161 TLLSVSPYGPYWRHVRKIATHELLANQRLEQLQHIRMSEVRSSIKKLYEL--NKGSGGKV 220

Query: 181 TVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDSFPFL 240
            VEM+ WF  +++NTILR+VVGKR  S F+G   E+Y++ALRD+  LFGAFVPSDSFP L
Sbjct: 221 RVEMRRWFGDVSLNTILRMVVGKRLSSVFEGSGAEQYKEALRDVFELFGAFVPSDSFPCL 280

Query: 241 SWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKK---KNEMERNEEDFLDVMLSIVEHH 300
           SWLD+GGYKKAM  TAKVLDQ+  KWIEE RE  K   +  +E  E+DF+DVMLS V  H
Sbjct: 281 SWLDLGGYKKAMNKTAKVLDQMLTKWIEEHREKNKNWGEGGVETEEQDFMDVMLSPVSDH 340

Query: 301 GEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQR 360
           G   GFD DTVTKATC  M+LG  DTTMITMTWALSLLLNNQ+ALKKAQLELD KVGR R
Sbjct: 341 GGLGGFDADTVTKATCFAMVLGAYDTTMITMTWALSLLLNNQQALKKAQLELDGKVGRSR 400

Query: 361 RVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQ 420
           +V+  DV NL YLQAIVKETLRLYP VPLLIPHES EDC VSGYHIPA+T+L+VNV+KLQ
Sbjct: 401 QVKEPDVKNLPYLQAIVKETLRLYPAVPLLIPHESVEDCTVSGYHIPARTQLLVNVKKLQ 460

Query: 421 QEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANL 480
           ++PL+WEDPC+FRPERFLTS   FD++GQN QL+PFGSGRRMCPGISFALQLMHLTLA+L
Sbjct: 461 EDPLVWEDPCKFRPERFLTSATKFDMRGQNLQLMPFGSGRRMCPGISFALQLMHLTLASL 520

Query: 481 LHEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQ 522
           LHEFEI RPS+E+LNM+E FG+T+LKKTPL+VV+ PRLP+ VY+
Sbjct: 521 LHEFEIDRPSEELLNMDEGFGITVLKKTPLEVVLHPRLPSLVYE 562

BLAST of PI0015183 vs. ExPASy TrEMBL
Match: A0A6J1IJB7 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476808 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 4.6e-217
Identity = 381/526 (72.43%), Postives = 437/526 (83.08%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPT 60
           MEL HIL ++F LLLFLY   RI+SRRS A +    LPPEA+G LPVIGHLHKLSATEPT
Sbjct: 1   MELTHILPATFALLLFLYALFRISSRRSAAHRTNKTLPPEAAGRLPVIGHLHKLSATEPT 60

Query: 61  HKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNY 120
           H TLAKMAD YGPIFTLRLGMNTAL+VSSWEIARECFTTND IFASRPK LASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALIVSSWEIARECFTTNDTIFASRPKGLASELLGYNY 120

Query: 121 AAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRN---N 180
             + +SPYGPYWRH+RKIAT ELL N RLEQ ++IR+SEV++SIK LYELCGDSRN    
Sbjct: 121 TVLSLSPYGPYWRHVRKIATHELLANQRLEQLQHIRMSEVRSSIKTLYELCGDSRNKGSG 180

Query: 181 NKATVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDSF 240
            K  VEM+TWF  +++NTILR+VVGKR    F+G   E+YR+A RD+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMRTWFGDVSLNTILRMVVGKRLSFVFEGSGAEQYREAFRDVFELFGAFVPSDSF 240

Query: 241 PFLSWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKN----EMERNEEDFLDVMLSI 300
           PFLSWLD+GGYKKAM  TAKVLDQ+  KWI+E RE K KN     +E  E+DF+DVMLS 
Sbjct: 241 PFLSWLDLGGYKKAMNKTAKVLDQMLTKWIQEHRE-KTKNWGECGVETKEQDFMDVMLSP 300

Query: 301 VEHHGEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKV 360
           V +HG    FDVDTVTKATC  M+LG  DTTMITMTWALSLLLN+ EAL+K QLELDEKV
Sbjct: 301 VSNHGGLGDFDVDTVTKATCFAMVLGAYDTTMITMTWALSLLLNHPEALRKVQLELDEKV 360

Query: 361 GRQRRVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNV 420
           GR R+V+ SDV NL YLQAI+KETLRLYP  PLLIPHES EDC V GYHIPA+TRLIVNV
Sbjct: 361 GRSRQVKESDVKNLLYLQAIMKETLRLYPAAPLLIPHESIEDCTVLGYHIPARTRLIVNV 420

Query: 421 QKLQQEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLT 480
           QKL ++ L+WE+PCEFRPERFLTS   FD++GQ+ QL+PFG GRRMCPGISFALQLMHLT
Sbjct: 421 QKLHKDSLVWENPCEFRPERFLTSDTKFDMRGQDLQLMPFGCGRRMCPGISFALQLMHLT 480

Query: 481 LANLLHEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKV 520
           LA+LLHEFEI RPS+E+L+M+E FG+T+LKKTPL+VV+ PRLP++V
Sbjct: 481 LASLLHEFEIDRPSEELLDMDEGFGITVLKKTPLEVVLHPRLPSQV 525

BLAST of PI0015183 vs. ExPASy TrEMBL
Match: A0A6J1F6A2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442569 PE=3 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 5.0e-216
Identity = 379/524 (72.33%), Postives = 435/524 (83.02%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPT 60
           ME  H  A++F LLLFLY   RI SRR  A    N LPPEA G+LP+IGHLHKLSAT+P 
Sbjct: 1   MEHTHSSAATFALLLFLYALFRIASRRPGAHSRNNTLPPEAGGALPLIGHLHKLSATQPP 60

Query: 61  HKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNY 120
           H TLAKMAD YGPIFTLRLGMN AL+VSSWEIARECFTTND IFASRPK LASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNKALIVSSWEIARECFTTNDSIFASRPKGLASELLGYNY 120

Query: 121 AAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKA 180
             + +SPYGPYWR +RKIATLELLTNHRL Q ++IR+SEVQ+SIKKLYEL  +  +  + 
Sbjct: 121 TVLSISPYGPYWRRVRKIATLELLTNHRLHQLQHIRISEVQSSIKKLYEL--NKGSGGRV 180

Query: 181 TVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDSFPFL 240
            VEM+ WF  +++NTI R+VVGKRF + F+G   E+YR+A RDL  LFGAFVPSDSFPFL
Sbjct: 181 RVEMRAWFGDVSLNTIFRMVVGKRFSTVFEGSGAEQYREAFRDLSELFGAFVPSDSFPFL 240

Query: 241 SWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKK---KNEMERNEEDFLDVMLSIVEHH 300
           SWLD+GGYKKAM  TAKV+DQ+  KWI+E RE KK   +  +E  E+DF+DVMLS V+  
Sbjct: 241 SWLDLGGYKKAMNKTAKVVDQMVTKWIKEHREKKKSSGEGGVETKEQDFMDVMLSTVDDQ 300

Query: 301 GEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQR 360
            EF GFD DTVTKATCL MILG +DTT +TMTWALSLLLNNQ+AL KAQLELDEKVGR R
Sbjct: 301 -EFCGFDADTVTKATCLMMILGASDTTTVTMTWALSLLLNNQQALNKAQLELDEKVGRSR 360

Query: 361 RVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQ 420
           +V+ SDV NL YLQAIVKET+RLYP  PLLIPHES EDC VSGYHIPAKTRLIVNVQKLQ
Sbjct: 361 QVKESDVKNLPYLQAIVKETMRLYPAAPLLIPHESAEDCTVSGYHIPAKTRLIVNVQKLQ 420

Query: 421 QEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANL 480
           ++P +WE PCEFRPERFLTS  +FD++GQN QL+PFG GRR+CPGISFALQLMHLTLANL
Sbjct: 421 KDPRVWEAPCEFRPERFLTSDTEFDMRGQNQQLMPFGCGRRICPGISFALQLMHLTLANL 480

Query: 481 LHEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQ 522
           LHEFEI RPS+E+LNM+ESFG+TILKKTPL+VV+ PRLP +VY+
Sbjct: 481 LHEFEIGRPSEELLNMDESFGITILKKTPLEVVLSPRLPNQVYE 521

BLAST of PI0015183 vs. NCBI nr
Match: XP_022935743.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 779.6 bits (2012), Expect = 1.7e-221
Identity = 388/523 (74.19%), Postives = 440/523 (84.13%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPT 60
           MEL H+L + F LLLFLY    ITSRRS A +   RLPPEA G LPVIGHLHKLSATEPT
Sbjct: 1   MELTHVLPAIFALLLFLYALFAITSRRSSAHRTNKRLPPEAGGRLPVIGHLHKLSATEPT 60

Query: 61  HKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNY 120
           H TLAKMAD YGPIFTLRLGMNTALVVSSWEIARECFTTND IFASR KA+ASELLGYNY
Sbjct: 61  HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDSIFASRSKAVASELLGYNY 120

Query: 121 AAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKA 180
             + +SPYGPYWRH+RKIATLELLTNHRL Q ++IR+SEV++SIKKLYEL  +  +  K 
Sbjct: 121 TVLSLSPYGPYWRHVRKIATLELLTNHRLHQLQHIRISEVRSSIKKLYEL--NKGSGGKV 180

Query: 181 TVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDSFPFL 240
            VEM+TWF   T+NTI R+V+GKRF + F+G  GE+YR+ALRDL+ L GAFVPSDSFPFL
Sbjct: 181 RVEMRTWFGDATLNTIFRMVMGKRFSTVFEGSGGEQYRQALRDLLELLGAFVPSDSFPFL 240

Query: 241 SWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKN--EMERNEEDFLDVMLSIVEHHG 300
           SWLD+GGYKKAM  TAKVLDQ+  KWI+E RE  K +    E  E+DF+D+MLS V   G
Sbjct: 241 SWLDLGGYKKAMNKTAKVLDQMLSKWIKEHREKTKTSGERGETEEQDFMDLMLSAVHDDG 300

Query: 301 EFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQRR 360
              GFD DTVTKATCLTMILGG+DTT +TMTWALSLLLNN++ALKKAQLELDEKVGR R 
Sbjct: 301 GLCGFDADTVTKATCLTMILGGSDTTTVTMTWALSLLLNNEQALKKAQLELDEKVGRSRH 360

Query: 361 VEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQQ 420
           V+ SDV NL YLQAIVKETLRLYP VPLL+PHES  DC+VSGYHIPAKTRLIVNVQKLQ+
Sbjct: 361 VKESDVKNLPYLQAIVKETLRLYPAVPLLVPHESMVDCMVSGYHIPAKTRLIVNVQKLQK 420

Query: 421 EPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANLL 480
           +PL+WEDP EFRPERFLTS   FD++GQN QL+PFGSGRRMCPGISFALQLMHLTLA+LL
Sbjct: 421 DPLVWEDPSEFRPERFLTSDTKFDMRGQNQQLMPFGSGRRMCPGISFALQLMHLTLASLL 480

Query: 481 HEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQ 522
           HEFEI RPS E+LNM+E FG+T+LKKTPL+VV+ PRLP +VY+
Sbjct: 481 HEFEIGRPSQELLNMDEGFGITVLKKTPLEVVLSPRLPIQVYE 521

BLAST of PI0015183 vs. NCBI nr
Match: XP_023535445.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 768.8 bits (1984), Expect = 2.9e-218
Identity = 382/524 (72.90%), Postives = 436/524 (83.21%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPT 60
           MEL HI+ ++F LLLFLY  +RITSRRS A +   RLPPEA+G LPVIGHLHKLSATEPT
Sbjct: 40  MELTHIIPATFALLLFLYALLRITSRRSAAHRTNKRLPPEAAGRLPVIGHLHKLSATEPT 99

Query: 61  HKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNY 120
           H TLAKMAD YGPIFTL+LGMNT LVVSSWEIARECFTTND IFASR K LASELLGYNY
Sbjct: 100 HITLAKMADAYGPIFTLKLGMNTVLVVSSWEIARECFTTNDTIFASRSKGLASELLGYNY 159

Query: 121 AAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKA 180
             + V+PYGPYWRH+RKIAT ELL N RLEQ ++IR+SEV++SIKKLYEL  +  +  K 
Sbjct: 160 TLLSVTPYGPYWRHVRKIATHELLANQRLEQLQHIRMSEVRSSIKKLYEL--NKGSGGKV 219

Query: 181 TVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDSFPFL 240
            VEM TWF  +++NTILR+VVGKR  S  +G   E+Y++A RD+  LFGAFVPSDSFPFL
Sbjct: 220 RVEMGTWFGDVSLNTILRMVVGKRLSSVLEGSGAEQYKEASRDVFELFGAFVPSDSFPFL 279

Query: 241 SWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKK---KNEMERNEEDFLDVMLSIVEHH 300
           SWLD+GGYKKAM  TAKVLDQ+  KWIEE RE  K   +  +E  E+DF+DVMLS V   
Sbjct: 280 SWLDLGGYKKAMNKTAKVLDQMLTKWIEEHREKNKNLGEGGVETEEQDFMDVMLSAVSDQ 339

Query: 301 GEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQR 360
           G   GFD DTVTKATC  M+LG  DTTMITMTWALSLLLNNQ+AL KAQLELDEKVG  R
Sbjct: 340 GGLGGFDADTVTKATCFAMVLGAYDTTMITMTWALSLLLNNQQALNKAQLELDEKVGTSR 399

Query: 361 RVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQ 420
           RV+ SDV NL YLQAI+KETLRLYP  PLLIPHES EDCIVSGYHIPA TRL+VNVQKLQ
Sbjct: 400 RVKESDVKNLPYLQAILKETLRLYPAAPLLIPHESVEDCIVSGYHIPAGTRLLVNVQKLQ 459

Query: 421 QEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANL 480
           + PL+WEDPCEFRPERFLTS   FD++GQN QL+PFGSGRRMCPGISFALQLMHLTLA+L
Sbjct: 460 KNPLVWEDPCEFRPERFLTSDTKFDMRGQNMQLMPFGSGRRMCPGISFALQLMHLTLASL 519

Query: 481 LHEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQ 522
           LHEFEI+RPS+E+LNM+E FG+T+LKKTPL+VV+ PRLP++VY+
Sbjct: 520 LHEFEINRPSEELLNMDEGFGITVLKKTPLEVVLHPRLPSQVYE 561

BLAST of PI0015183 vs. NCBI nr
Match: XP_022936281.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 767.7 bits (1981), Expect = 6.5e-218
Identity = 388/523 (74.19%), Postives = 436/523 (83.37%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRN-RLPPEASGSLPVIGHLHKLSATEP 60
           MEL HIL ++F LLLFLY   RI SRRS A    N R PPEA+G+LP+IGHLHKLSATEP
Sbjct: 1   MELTHILPATFALLLFLYALFRIASRRSSAAHRSNKRHPPEAAGALPLIGHLHKLSATEP 60

Query: 61  THKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYN 120
           TH TLAKMAD YGPIFTLRLGMNTALVVSSWEIARECFTTND IFASR K +ASELLGYN
Sbjct: 61  THITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRSKVVASELLGYN 120

Query: 121 YAAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRN--- 180
           YAA+G+SPYGPYWRH+RK+ATLELLTNHRLEQ ++IR+ EVQ SIKKLYELC DSRN   
Sbjct: 121 YAALGISPYGPYWRHVRKLATLELLTNHRLEQLQHIRICEVQNSIKKLYELCADSRNKGS 180

Query: 181 NNKATVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDS 240
             K  VEM+ WF  +++NTI R+VVGKRF + F+G   E+YR+ALR    LF AFVPSD 
Sbjct: 181 GGKVRVEMRRWFGDVSLNTIFRMVVGKRFSAVFEGSGAEQYREALRAFFELFAAFVPSDL 240

Query: 241 FPFLSWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKK---KNEMERNEEDFLDVMLSI 300
           FPFLSWLD+GGYKKAM  TAKVLDQ+  KWIEE RE KK   +  +E  E+DF+D+MLS 
Sbjct: 241 FPFLSWLDLGGYKKAMNKTAKVLDQMLSKWIEEHREKKKNWGEGGVETEEQDFMDLMLSA 300

Query: 301 VEHHGEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKV 360
           V   G   G   DTVTKATCLTMILGG DTT ITMTWALSLLLN+QEALKKAQLELDEK+
Sbjct: 301 VHDDGGLCGIGGDTVTKATCLTMILGGLDTTTITMTWALSLLLNDQEALKKAQLELDEKI 360

Query: 361 GRQRRVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNV 420
           GR R+V+ SDV NLTYLQAIVKETLRLYP  PLLIPHES EDCIVSGYHIPA+TRL+VNV
Sbjct: 361 GRSRQVKESDVKNLTYLQAIVKETLRLYPAAPLLIPHESVEDCIVSGYHIPARTRLLVNV 420

Query: 421 QKLQQEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLT 480
           QKLQ++PL+WEDPCEFRPERFL SH  FDL+GQN QL+PFGSGRR CPGISF+LQLMHLT
Sbjct: 421 QKLQKDPLVWEDPCEFRPERFLISHTKFDLRGQNHQLMPFGSGRRKCPGISFSLQLMHLT 480

Query: 481 LANLLHEFEISRPSDEMLNM-EESFGLTILKKTPLQVVVCPRL 516
           LANLLHEFEI RPS+++L+  EE FGLT +KKTPL+VV+ PRL
Sbjct: 481 LANLLHEFEIGRPSEKLLDKEEEGFGLTGVKKTPLEVVLSPRL 523

BLAST of PI0015183 vs. NCBI nr
Match: XP_023535446.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.3 bits (1980), Expect = 8.5e-218
Identity = 383/527 (72.68%), Postives = 436/527 (82.73%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPT 60
           MEL HIL ++F LLLFLY    ITSRRS A +  N LPPE +G LP+IGHLHKLSATEPT
Sbjct: 1   MELTHILPATFALLLFLYALFAITSRRSAAHRTNNTLPPEPAGRLPLIGHLHKLSATEPT 60

Query: 61  HKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNY 120
           H TLAKMAD YGPIFTLRLG+NTALVVSSWEIARECFTTND IFASRPK LA ELLGYN 
Sbjct: 61  HITLAKMADAYGPIFTLRLGVNTALVVSSWEIARECFTTNDTIFASRPKGLAPELLGYNS 120

Query: 121 AAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSR---NN 180
           A M +SPYGPYWRH+RKIATLELLTNHRLEQ ++IR+SEVQ+SIKKLYELC DS    + 
Sbjct: 121 AMMAISPYGPYWRHVRKIATLELLTNHRLEQLQHIRISEVQSSIKKLYELCADSTSKGSG 180

Query: 181 NKATVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDSF 240
            K  VEM+TWF  +++NTI R++VGKRF +  +G  GEKYR+ALRD+  LFGAFVPSDSF
Sbjct: 181 GKVRVEMETWFGDVSLNTIFRMMVGKRFSTDSEGSGGEKYRQALRDVFELFGAFVPSDSF 240

Query: 241 PFLSWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKK---KNEMERNEEDFLDVMLSIV 300
           P L WLD+GGYKKAM  TAKVLDQ+  KWIEE RE KK   +  +E  E+DF+DVMLS V
Sbjct: 241 PCLGWLDLGGYKKAMNKTAKVLDQMLTKWIEEHREKKKNLGEGGVETKEQDFMDVMLSAV 300

Query: 301 EHHGEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVG 360
              G   GFD DTVTKATCL ++LG +DTTMITMTWALSLLLNNQ+ALKKAQLELDEKVG
Sbjct: 301 HDDGGLCGFDADTVTKATCLAIVLGASDTTMITMTWALSLLLNNQQALKKAQLELDEKVG 360

Query: 361 RQRRVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQ 420
             R V+ SDV NLTYLQAIVKETLRL+P  PLLIPHE  EDC VSGYHIPA+TRL+VNVQ
Sbjct: 361 ISRHVKESDVKNLTYLQAIVKETLRLHPAAPLLIPHEFVEDCTVSGYHIPARTRLLVNVQ 420

Query: 421 KLQQEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTL 480
           KLQ++PL+WEDPCEFRPERFLTS   FD++GQN QL+PFGSGRRMCPGISFALQLMHLTL
Sbjct: 421 KLQKDPLVWEDPCEFRPERFLTSDTKFDMRGQNQQLMPFGSGRRMCPGISFALQLMHLTL 480

Query: 481 ANLLHEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQ 522
           ANLL +FEI RPS E+L+MEE FG T+ ++TPL+VV+ PRL  + Y+
Sbjct: 481 ANLLQKFEIERPSQELLDMEEGFGSTVPRRTPLEVVLSPRLSNQFYE 527

BLAST of PI0015183 vs. NCBI nr
Match: XP_022935737.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 765.4 bits (1975), Expect = 3.2e-217
Identity = 381/524 (72.71%), Postives = 436/524 (83.21%), Query Frame = 0

Query: 1   MELPHILASSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPT 60
           MEL H + ++F LLLFLY   RI+SRRS A +  N LPPEA G LPVIGHLHKLSATEPT
Sbjct: 41  MELTHNIPATFALLLFLYALFRISSRRSAAHRTNNTLPPEAGGRLPVIGHLHKLSATEPT 100

Query: 61  HKTLAKMADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNY 120
           H TLAKMAD YGPIFTLRLGMNTALVVSSWEIARECFTTND IFASR K LASELLGYNY
Sbjct: 101 HITLAKMADAYGPIFTLRLGMNTALVVSSWEIARECFTTNDTIFASRSKGLASELLGYNY 160

Query: 121 AAMGVSPYGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKA 180
             + VSPYGPYWRH+RKIAT ELL N RLEQ ++IR+SEV++SIKKLYEL  +  +  K 
Sbjct: 161 TLLSVSPYGPYWRHVRKIATHELLANQRLEQLQHIRMSEVRSSIKKLYEL--NKGSGGKV 220

Query: 181 TVEMKTWFEKLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDSFPFL 240
            VEM+ WF  +++NTILR+VVGKR  S F+G   E+Y++ALRD+  LFGAFVPSDSFP L
Sbjct: 221 RVEMRRWFGDVSLNTILRMVVGKRLSSVFEGSGAEQYKEALRDVFELFGAFVPSDSFPCL 280

Query: 241 SWLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKK---KNEMERNEEDFLDVMLSIVEHH 300
           SWLD+GGYKKAM  TAKVLDQ+  KWIEE RE  K   +  +E  E+DF+DVMLS V  H
Sbjct: 281 SWLDLGGYKKAMNKTAKVLDQMLTKWIEEHREKNKNWGEGGVETEEQDFMDVMLSPVSDH 340

Query: 301 GEFPGFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQR 360
           G   GFD DTVTKATC  M+LG  DTTMITMTWALSLLLNNQ+ALKKAQLELD KVGR R
Sbjct: 341 GGLGGFDADTVTKATCFAMVLGAYDTTMITMTWALSLLLNNQQALKKAQLELDGKVGRSR 400

Query: 361 RVEASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQ 420
           +V+  DV NL YLQAIVKETLRLYP VPLLIPHES EDC VSGYHIPA+T+L+VNV+KLQ
Sbjct: 401 QVKEPDVKNLPYLQAIVKETLRLYPAVPLLIPHESVEDCTVSGYHIPARTQLLVNVKKLQ 460

Query: 421 QEPLIWEDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANL 480
           ++PL+WEDPC+FRPERFLTS   FD++GQN QL+PFGSGRRMCPGISFALQLMHLTLA+L
Sbjct: 461 EDPLVWEDPCKFRPERFLTSATKFDMRGQNLQLMPFGSGRRMCPGISFALQLMHLTLASL 520

Query: 481 LHEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQ 522
           LHEFEI RPS+E+LNM+E FG+T+LKKTPL+VV+ PRLP+ VY+
Sbjct: 521 LHEFEIDRPSEELLNMDEGFGITVLKKTPLEVVLHPRLPSLVYE 562

BLAST of PI0015183 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 486.1 bits (1250), Expect = 3.5e-137
Identity = 246/522 (47.13%), Postives = 355/522 (68.01%), Query Frame = 0

Query: 9   SSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEP-THKTLAKM 68
           S F+L + +  FV I   +  +++ +    P  SG+ P+IGHLH L   E   ++TL KM
Sbjct: 4   SLFSLFVPILVFVFIALFKK-SKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKM 63

Query: 69  ADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNYAAMGVSP 128
           AD YGP  +L+LG N A VVSS+E+A++CFT ND+  ASRP   A++ +GYN+A  G +P
Sbjct: 64  ADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAP 123

Query: 129 YGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKATVEMKTW 188
           Y  +WR +RKIAT+ELL+N RL+  +++R+SE+   +K LY L   +       V++K+W
Sbjct: 124 YSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSW 183

Query: 189 FEKLTMNTILRLVVGKRFDSAFQGIDGE------KYRKALRDLIYLFGAFVPSDSFPFLS 248
            E +T+N I+R+V GKR+      +  E      + +KA+    +L G F  SD+FP LS
Sbjct: 184 LEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLS 243

Query: 249 WLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKNEMERNEEDFLDVMLSIVEHHGEFP 308
           + D+ G++K MK T   LD + ++WIE  R+ +K +  + N+ DF+DVM+S+ E  G+  
Sbjct: 244 FFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAE-QGKLS 303

Query: 309 --GFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQRRV 368
              +D +T  K+TCL +ILGG+DT+  T+TWA+SLLLNN+E LKKAQ E+D  VGR R V
Sbjct: 304 HLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNV 363

Query: 369 EASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQQE 428
           E SD+ NL YLQAI+KETLRLYP  PLL P E+ EDC V+GY++P  TRLIVNV K+Q++
Sbjct: 364 EDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRD 423

Query: 429 PLIWEDPCEFRPERFLTSH-KDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANLL 488
           P ++ +P EFRPERF+T   K+FD++GQNF+L+PFGSGRR CPG S A+Q++HL LA  L
Sbjct: 424 PKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFL 483

Query: 489 HEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVY 521
           H F++    D  ++M E+ GLTI K TPL+V++ PR+  +++
Sbjct: 484 HSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523

BLAST of PI0015183 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 466.5 bits (1199), Expect = 2.9e-131
Identity = 239/521 (45.87%), Postives = 350/521 (67.18%), Query Frame = 0

Query: 9   SSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEP-THKTLAKM 68
           S F+L + +  FV I   +  +++ ++   P  SG+ P+IGHLH LS  E   ++TL KM
Sbjct: 4   SLFSLFVPILVFVFIALFKK-SKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKM 63

Query: 69  ADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNYAAMGVSP 128
           AD YGP  +LRLG +   VVSS+E+A++CFT ND+  ASRP   A++ +GY+ A  G +P
Sbjct: 64  ADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAP 123

Query: 129 YGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKATVEMKTW 188
           Y  +WR +RKIATLELL+N RL+  +++R+SE+   ++ LY L      +    V++K+W
Sbjct: 124 YSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSW 183

Query: 189 FEKLTMNTILRLVVGKRFD-----SAFQGIDGEKYRKALRDLIYLFGAFVPSDSFPFLSW 248
            E +++N ++R+V GKR+      S     +  + RK + +  +L G F  SD+FP L W
Sbjct: 184 LEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGW 243

Query: 249 LDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKNEMERNEEDFLDVMLSIVEHHGEFPG 308
            D  G++K MK T + LD + ++WIE  R+ +K +  + N+ DF+DVMLS+ E  G+F  
Sbjct: 244 FDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAE-QGKFSH 303

Query: 309 FDVDTVT--KATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQRRVE 368
              D +T  K+TCL +ILGG++T+  T+TWA+SLLLNN++ LKKAQ E+D  VGR R VE
Sbjct: 304 LQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVE 363

Query: 369 ASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQQEP 428
            SD+ NL Y+QAI+KETLRLYP  PLL   E+ EDC V+GY++   TR++VNV K+Q++P
Sbjct: 364 DSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDP 423

Query: 429 LIWEDPCEFRPERFLTSH-KDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANLLH 488
            ++ +P EFRPERF+T   K+FD++GQNF+L+PFGSGRR CPG S A+Q++HL LA  L 
Sbjct: 424 RVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQ 483

Query: 489 EFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVY 521
            F++    D  ++M ES GLTI K TPL++++ PRL   +Y
Sbjct: 484 SFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of PI0015183 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 445.3 bits (1144), Expect = 6.9e-125
Identity = 237/522 (45.40%), Postives = 339/522 (64.94%), Query Frame = 0

Query: 9   SSFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEP-THKTLAKM 68
           S F+L + +  FV I   +  +++ +    P  SG+ P+IGHLH L   E   ++TL KM
Sbjct: 4   SLFSLFVSILVFVFIALFKK-SKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKM 63

Query: 69  ADVYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNYAAMGVSP 128
           AD YGP  +LRLG +   V SS+E+A++CFT ND+  AS   A A++ +GY         
Sbjct: 64  ADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASLMTA-AAKHMGY--------- 123

Query: 129 YGPYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKATVEMKTW 188
              +W  +RKIA +ELL+N RL+    +R+SE+   +K LY L      +    V++K+W
Sbjct: 124 --VFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSW 183

Query: 189 FEKLTMNTILRLVVGKRF------DSAFQGIDGEKYRKALRDLIYLFGAFVPSDSFPFLS 248
            E +  N I+R+V GKR+      +S+    +  ++RK +    +L G F  SD+FP L 
Sbjct: 184 LEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLG 243

Query: 249 WLDVGGYKKAMKNTAKVLDQVFDKWIEERREMKKKNEMERNEEDFLDVMLSIVEHHGEFP 308
           WLD+ G++K MK T + LD + ++WIE  R+ +K +  + N+ DF+DVMLS+ E  G+  
Sbjct: 244 WLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAE-QGKLS 303

Query: 309 --GFDVDTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQRRV 368
              +D +T  K TCL +ILGG++T+  T+TWA+SLLLNN++ LKK Q E+D  VGR R V
Sbjct: 304 HLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNV 363

Query: 369 EASDVTNLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQQE 428
           E SD+ NL YLQAI+KETLRLYP  PLL   E+ EDC V+GY++P  TRLIVNV K+Q++
Sbjct: 364 EDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRD 423

Query: 429 PLIWEDPCEFRPERFLTSH-KDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANLL 488
           P ++ +P EFRPERF+T   KDFD++GQNF+L+PFGSGRR CPG S A+Q++HL LA  L
Sbjct: 424 PKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFL 483

Query: 489 HEFEISRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVY 521
           H FE+    D  ++M ES GLTI K TPL+V++ PRL  +++
Sbjct: 484 HSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511

BLAST of PI0015183 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 394.8 bits (1013), Expect = 1.1e-109
Identity = 222/514 (43.19%), Postives = 305/514 (59.34%), Query Frame = 0

Query: 10  SFTLLLFLYTFVRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPTHKTLAKMAD 69
           S  L++F Y F+R    R    ++ +   PE  G+LP+ GHLH L   +   K LA M+ 
Sbjct: 14  SLALVIFGYIFLRKQLSRC---EVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQ 73

Query: 70  VYGPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNYAAMGVSPYG 129
            +GPIF+L+LG    +V S  +  ++CFTTND   A+RP       +GYN A++ ++PYG
Sbjct: 74  KHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYG 133

Query: 130 PYWRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNKATVEMKTWFE 189
            YWR +RKI T+ L +NH +E   +IR SEV T IK LY+      N   + V++   FE
Sbjct: 134 DYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYK-----GNGGTSIVKIDMLFE 193

Query: 190 KLTMNTILRLVVGKRFDSAFQGIDGEKYRKALRDLIYLFGAFVPSDSFPFLSWLDVGGYK 249
            LT N ILR +VGKR        D  +Y++AL+   YL    +  D  P+L WLD     
Sbjct: 194 FLTFNIILRKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDF-AKN 253

Query: 250 KAMKNTAKVLDQVFDKWIEERREMKKKNEMERNEEDFLDVMLSIVEHHGEFPGFDVDTVT 309
             MK   K LD V  KW+ E  + + +NE ++ E   +D++L I+       G   D + 
Sbjct: 254 SQMKRLFKELDSVNTKWLHEHLKKRSRNEKDQ-ERTIMDLLLDILPEDIVISGHVRDVIV 313

Query: 310 KATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQRRVEASDVTNLTY 369
           KAT L + L G+D+T IT+TWA+SLLLNN  AL+ AQ E+D  VG+ R +E SD+ NL Y
Sbjct: 314 KATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKY 373

Query: 370 LQAIVKETLRLYPPVPLLIPHESTEDCIVSGYHIPAKTRLIVNVQKLQQEPLIWEDPCEF 429
           LQAIVKET RLYPP PL    E+ EDC V GY +   TRL+VN+ KL ++P IW DP  F
Sbjct: 374 LQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTF 433

Query: 430 RPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANLLHEFEISRPSDE 489
           +PERF+        +  NF+ IPFGSGRR CPG++  L+++H  LA LL  FE+ + SDE
Sbjct: 434 KPERFMEDKS--QCEKSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDE 493

Query: 490 MLNMEESFGLTILKKTPLQVVVCPRLPTKVYQCM 524
            L+M E  GL + K  P++VVV PRL  K+Y  +
Sbjct: 494 PLDMAEGPGLALPKINPVEVVVMPRLDPKLYSLL 515

BLAST of PI0015183 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 374.8 bits (961), Expect = 1.1e-103
Identity = 209/518 (40.35%), Postives = 313/518 (60.42%), Query Frame = 0

Query: 13  LLLFLYTF-VRITSRRSIAQQMRNRLPPEASGSLPVIGHLHKLSATEPTHKTLAKMADVY 72
           LL  L+ F V I  +  +  + +    P   G+ P++GHLH      PTH T   MADVY
Sbjct: 8   LLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVY 67

Query: 73  GPIFTLRLGMNTALVVSSWEIARECFTTNDRIFASRPKALASELLGYNYAAMGVSPYGPY 132
           GP+F  +LG    ++++S E+A+E +T +D++   RP+  AS+LLGYN + +  SPYG Y
Sbjct: 68  GPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSPYGLY 127

Query: 133 WRHIRKIATLELLTNHRLEQFRYIRLSEVQTSIKKLYELCGDSRNNNK--ATVEMKTWFE 192
           WR IRKIA  EL +   ++     R  E   + + LY +  + R   K    V+MK  F 
Sbjct: 128 WREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALY-VRWEKRGKPKEGVLVDMKQEFI 187

Query: 193 KLTMNTILRLVVGKRFDSAFQGIDGEKYR---KALRDLIYLFGAFVPSDSFPFLSWLDVG 252
            LT N  L +V GKR+       + ++ R   K +R+ +  F  F+ SD  P L +LD  
Sbjct: 188 DLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLD-W 247

Query: 253 GYKKAMKNTAKVLDQVFDKWIEERREMKKKNEMERNEEDFLDVMLSIVEHHGEFPGF-DV 312
             K+ MK TAK LD+V + WIEE +   K+++  R+E D+LD+++ I+    + PG  D 
Sbjct: 248 KTKRGMKRTAKGLDKVAEGWIEEHK--NKRSDHGRSENDYLDILIKIL-GQDKIPGLSDT 307

Query: 313 DTVTKATCLTMILGGADTTMITMTWALSLLLNNQEALKKAQLELDEKVGRQRRVEASDVT 372
            T  KA CL ++L G++T ++ + WA+SLLLNN   L+KAQ ELD K+G++R VE  D+ 
Sbjct: 308 HTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIK 367

Query: 373 NLTYLQAIVKETLRLYPPVPLLIPHESTEDCIVS--GYHIPAKTRLIVNVQKLQQEPLIW 432
           +L YLQAIVKET RLYPPVPL+      ED  ++    H+PA T+L+V+  K+ ++P +W
Sbjct: 368 DLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVW 427

Query: 433 EDPCEFRPERFLTSHKDFDLKGQNFQLIPFGSGRRMCPGISFALQLMHLTLANLLHEFEI 492
            +P +F PERFLTS+++ D+ GQ+++  PFG GRR CP I   ++++H  L   LH F++
Sbjct: 428 SNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDL 487

Query: 493 SRPSDEMLNMEESFGLTILKKTPLQVVVCPRLPTKVYQ 522
           +RPS + ++M ES GL   K TPL+V + PRL   +Y+
Sbjct: 488 ARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLYE 519