PI0014916 (gene) Melon (PI 482460) v1

Overview
NamePI0014916
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionReverse transcriptase
Locationchr12: 14399512 .. 14400418 (+)
RNA-Seq ExpressionPI0014916
SyntenyPI0014916
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGATGATGTTGGAAAAATGTTTTTAGGTCATGGATGTCTTGGAGAGCGAAAGGTAAACCTTGCCACATTTCTATTTCAAAAAGAGGCAGAGAATTTGGTGGAGGTCAATCGAGGTAAGACGAAGTGATGCAAACATTCTTGATTGGAAAACTTTCAAAGTATTTTTTAGGATACGTACTATCCTAGTACCTATTGTAAGTTAAAAAGAGATGAATTTCTAAGTTTGGAACAAAGATCGCTTTCGATGACGAAATATAAAAGGAAGATAACTAAAATTTCTAGGTATGCTGAAGTGTTAGTGGCAATAGAGGTTGACTGTTGTCGTAAGTTCGAAAGACGTTTAAGGTAAGAAATTCTTACCCCTGTTACTGCAACAGCAAAGTGGAATGACTTCTCCCAGTTGGTTGAGACTGCATTACGGGTTGAACAAGGTCTCTTGAAGACTAAACCAACAATGGAATCAGGTCAAGGGACTTCATTGTTTAGTAATACTAAAGGATGGGAGCAACAACAATTTACACCTGGTGTGTCGAGTAAACAAGATTTTAAAACTCAATCTGGTGAACAGTTTTTCAGACAACGAAGGAAGTGTTTTTCTAAGACCATCAGTTAAAGAAGTAACGATGTCTCGATCAGGACAGGAATCTACAACAAGTAATCAAGATAGATCTCCGTGTGGATATTGTGGAAGATTCCATAGAGGATCATGTTTGGTAAAAGTAGGAGAATGTCACTACTGTAAGAAACTAGGACATTTCATGAGGGAATGTCCGTTGTTGAAAAGAGAATTGCAGGAAAACCAGAAAGAAGAGTCTCAGTTAATGAATCAACAGAAGACTACACCAGTTGGAAAAGAAGAACCCAGTGACACCAAGCAAAAAGGTGTAGGACGACCTCGATAG

mRNA sequence

ATGGTTGATGATGTTGGAAAAATGTTTTTAGGTCATGGATGTCTTGGAGAGCGAAAGGTAAACCTTGCCACATTTCTATTTCAAAAAGAGGCAGAGAATTTGGTGGAGGTCAATCGAGCAAAGTGGAATGACTTCTCCCAGTTGGTTGAGACTGCATTACGGGTTGAACAAGGTCTCTTGAAGACTAAACCAACAATGGAATCAGTAATACTAAAGGATGGGAGCAACAACAATTTACACCTGGTGTGTCGAGTAAACAAGATTTTAAAACTCAATCTGGTGAACAGTTTTTCAGACAACGAAGGAAGTGTTTTTCTAAGACCATCAGTTAAAGAAGTAACGATGTCTCGATCAGGACAGGAATCTACAACAAGTAATCAAGATAGATCTCCGTGTGGATATTGTGGAAGATTCCATAGAGGATCATGTTTGGTAAAAGTAGGAGAATGTCACTACTGTAAGAAACTAGGACATTTCATGAGGGAATGTCCGTTGTTGAAAAGAGAATTGCAGGAAAACCAGAAAGAAGAGTCTCAGTTAATGAATCAACAGAAGACTACACCAGTTGGAAAAGAAGAACCCAGTGACACCAAGCAAAAAGGTGTAGGACGACCTCGATAG

Coding sequence (CDS)

ATGGTTGATGATGTTGGAAAAATGTTTTTAGGTCATGGATGTCTTGGAGAGCGAAAGGTAAACCTTGCCACATTTCTATTTCAAAAAGAGGCAGAGAATTTGGTGGAGGTCAATCGAGCAAAGTGGAATGACTTCTCCCAGTTGGTTGAGACTGCATTACGGGTTGAACAAGGTCTCTTGAAGACTAAACCAACAATGGAATCAGTAATACTAAAGGATGGGAGCAACAACAATTTACACCTGGTGTGTCGAGTAAACAAGATTTTAAAACTCAATCTGGTGAACAGTTTTTCAGACAACGAAGGAAGTGTTTTTCTAAGACCATCAGTTAAAGAAGTAACGATGTCTCGATCAGGACAGGAATCTACAACAAGTAATCAAGATAGATCTCCGTGTGGATATTGTGGAAGATTCCATAGAGGATCATGTTTGGTAAAAGTAGGAGAATGTCACTACTGTAAGAAACTAGGACATTTCATGAGGGAATGTCCGTTGTTGAAAAGAGAATTGCAGGAAAACCAGAAAGAAGAGTCTCAGTTAATGAATCAACAGAAGACTACACCAGTTGGAAAAGAAGAACCCAGTGACACCAAGCAAAAAGGTGTAGGACGACCTCGATAG

Protein sequence

MVDDVGKMFLGHGCLGERKVNLATFLFQKEAENLVEVNRAKWNDFSQLVETALRVEQGLLKTKPTMESVILKDGSNNNLHLVCRVNKILKLNLVNSFSDNEGSVFLRPSVKEVTMSRSGQESTTSNQDRSPCGYCGRFHRGSCLVKVGECHYCKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTKQKGVGRPR
Homology
BLAST of PI0014916 vs. ExPASy TrEMBL
Match: A0A5A7UZA9 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G002660 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 2.2e-18
Identity = 71/175 (40.57%), Postives = 93/175 (53.14%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTMESVILKDGSNNNLHLVCRVNKILKLNLVNSFSD 99
           AKW +FSQLVETALRVEQ + + K  +E   L  G++       R           S + 
Sbjct: 174 AKWTNFSQLVETALRVEQSITEEKSAVE---LSRGTSTASGFRGR---------QASRNV 233

Query: 100 NEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRFHRGSCLVKVGECHY 159
           + GSVF R S       ++    S+ GQES  S   R+PC  CGR HRG CLV  G C+ 
Sbjct: 234 SYGSVFQRQSQRIPSQPIRSTVRSQPGQESIASTVRRTPCTSCGRNHRGQCLVGAGVCYQ 293

Query: 160 CKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTKQKG-VGRPR 207
           C + GHF ++CP L   +Q +Q   SQ + Q + + V  E  S  +QKG VGRPR
Sbjct: 294 CGQPGHFKKDCPQLNMTVQRDQGVGSQTVEQSRVSVVPIEGTSGARQKGVVGRPR 336

BLAST of PI0014916 vs. ExPASy TrEMBL
Match: A0A5A7V2E0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold108G002150 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 2.2e-18
Identity = 74/189 (39.15%), Postives = 98/189 (51.85%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTMESVILKDGSNNNLHL-VCRVNKILKLNLVNSFS 99
           AKW +FSQLVETALRVEQ + + K    +V L  G++    L  C   +      ++S  
Sbjct: 216 AKWTNFSQLVETALRVEQSITEEK---SAVKLSRGTSTASGLRGCEQRRFTPGINISSRQ 275

Query: 100 D-------------NEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRF 159
           D             + GSVF R S       ++    S+ GQES  S   R PC  CGR 
Sbjct: 276 DFKNRSGGQASRNVSYGSVFQRHSQRIPSQPIRSTVRSQPGQESIASTVRRLPCTSCGRN 335

Query: 160 HRGSCLVKVGECHYCKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTK 207
           HRG CLV VG C+ C + GHF ++CP L   +Q +Q   SQ + Q + + V  E+ S  +
Sbjct: 336 HRGQCLVGVGVCYQCGQPGHFKKDCPQLNMTVQRDQGVGSQTVEQSRVSVVPTEDTSGAR 395

BLAST of PI0014916 vs. ExPASy TrEMBL
Match: A0A5A7VQD2 (Putative Gag-pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2484G00150 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 6.5e-18
Identity = 71/186 (38.17%), Postives = 94/186 (50.54%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTME-----SVILKDGSNNNLHLVCRVNKILKLNLV 99
           AKW +FSQLVETA+RVEQ + + K  +E     S                +N   + +  
Sbjct: 216 AKWTNFSQLVETAIRVEQSITEEKSAVELSRGTSTASGFRGREQRRFTPGINISSRQDFK 275

Query: 100 N------SFSDNEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRFHRG 159
           N      S + + GSVF R S       ++    S+ GQES  S   R PC  CGR HRG
Sbjct: 276 NRSGGQASRNVSYGSVFQRQSQRIPSQPIRSTVRSQPGQESIASTVRRIPCTSCGRNHRG 335

Query: 160 SCLVKVGECHYCKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTKQKG 207
            CLV  G C+ C + GHF ++CP LK  +Q +Q   SQ + Q + + V  E  S  +QKG
Sbjct: 336 QCLVGAGVCYQCGQPGHFKKDCPQLKMTVQRDQGVGSQTIEQSRVSVVPTEGTSGARQKG 395

BLAST of PI0014916 vs. ExPASy TrEMBL
Match: A0A5D3BTP3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00020 PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 8.5e-18
Identity = 69/189 (36.51%), Postives = 97/189 (51.32%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTMESVILKDGSNNNLHLVCRVNKILKLNL-VNSFS 99
           AKW +FSQLVETALRV+Q +++ K  ME   L  G +    +  R  +     + V+   
Sbjct: 428 AKWMNFSQLVETALRVKQSIVEEKSAME---LSRGVSTTSGIRGREQRRFTPGVNVSGCQ 487

Query: 100 D-------------NEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRF 159
           D             + GS + R S          V  SR+GQES  S   R+PC  CG+ 
Sbjct: 488 DFKRRSGGKPLRQMSSGSAYQRQSRRASSQPANSVARSRTGQESVASESKRTPCVSCGKS 547

Query: 160 HRGSCLVKVGECHYCKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTK 207
           H+G C++  G C+ C K GHF R+CP L+  ++ +Q  ES  + Q + +    EE S  +
Sbjct: 548 HQGRCVIGAGVCYQCGKTGHFKRDCPQLRVVVRRDQGVESHTVEQSRISTAAGEETSGAR 607

BLAST of PI0014916 vs. ExPASy TrEMBL
Match: A0A5D3C7J1 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold594G00290 PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 8.5e-18
Identity = 70/189 (37.04%), Postives = 99/189 (52.38%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTMESVILKDGSNNNLHLVCRVNKILKLNL-VNSFS 99
           AKW +FSQLVETALRVEQ + + K  +E   L  G++       R  +   L + ++S  
Sbjct: 437 AKWTNFSQLVETALRVEQSITEEKSAVE---LSRGTSTASGFRGREQRRFTLGINISSRQ 496

Query: 100 D-------------NEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRF 159
           D             + GSVF R S       ++    S+ GQ+S  S+  ++PC  CGR 
Sbjct: 497 DFKNRSGGQASRNVSYGSVFQRQSQRIPSQPIRSTVRSQPGQKSIASSVKQTPCSSCGRN 556

Query: 160 HRGSCLVKVGECHYCKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTK 207
           HRG CLV  G C+ C + GHF ++CP L   +Q +Q   SQ + Q + + V  E  S  +
Sbjct: 557 HRGQCLVGAGVCYQCGQPGHFKKDCPQLNMTVQRDQGVGSQTVEQSRVSVVPTEGTSGAR 616

BLAST of PI0014916 vs. NCBI nr
Match: KAA0060440.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 102.4 bits (254), Expect = 4.6e-18
Identity = 71/175 (40.57%), Postives = 93/175 (53.14%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTMESVILKDGSNNNLHLVCRVNKILKLNLVNSFSD 99
           AKW +FSQLVETALRVEQ + + K  +E   L  G++       R           S + 
Sbjct: 174 AKWTNFSQLVETALRVEQSITEEKSAVE---LSRGTSTASGFRGR---------QASRNV 233

Query: 100 NEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRFHRGSCLVKVGECHY 159
           + GSVF R S       ++    S+ GQES  S   R+PC  CGR HRG CLV  G C+ 
Sbjct: 234 SYGSVFQRQSQRIPSQPIRSTVRSQPGQESIASTVRRTPCTSCGRNHRGQCLVGAGVCYQ 293

Query: 160 CKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTKQKG-VGRPR 207
           C + GHF ++CP L   +Q +Q   SQ + Q + + V  E  S  +QKG VGRPR
Sbjct: 294 CGQPGHFKKDCPQLNMTVQRDQGVGSQTVEQSRVSVVPIEGTSGARQKGVVGRPR 336

BLAST of PI0014916 vs. NCBI nr
Match: KAA0059911.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 102.4 bits (254), Expect = 4.6e-18
Identity = 74/189 (39.15%), Postives = 98/189 (51.85%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTMESVILKDGSNNNLHL-VCRVNKILKLNLVNSFS 99
           AKW +FSQLVETALRVEQ + + K    +V L  G++    L  C   +      ++S  
Sbjct: 216 AKWTNFSQLVETALRVEQSITEEK---SAVKLSRGTSTASGLRGCEQRRFTPGINISSRQ 275

Query: 100 D-------------NEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRF 159
           D             + GSVF R S       ++    S+ GQES  S   R PC  CGR 
Sbjct: 276 DFKNRSGGQASRNVSYGSVFQRHSQRIPSQPIRSTVRSQPGQESIASTVRRLPCTSCGRN 335

Query: 160 HRGSCLVKVGECHYCKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTK 207
           HRG CLV VG C+ C + GHF ++CP L   +Q +Q   SQ + Q + + V  E+ S  +
Sbjct: 336 HRGQCLVGVGVCYQCGQPGHFKKDCPQLNMTVQRDQGVGSQTVEQSRVSVVPTEDTSGAR 395

BLAST of PI0014916 vs. NCBI nr
Match: KAA0045363.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 102.1 bits (253), Expect = 6.0e-18
Identity = 72/186 (38.71%), Postives = 94/186 (50.54%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTME-----SVILKDGSNNNLHLVCRVNKILKLNLV 99
           AKW +FSQLVETALRVEQ + K K T+E     S                +N   + +  
Sbjct: 216 AKWTNFSQLVETALRVEQSITKEKSTVELSRGTSTASGFRGREQRRFTPGINISSRQDFK 275

Query: 100 N------SFSDNEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRFHRG 159
           N      S + + GSVF R S       ++    S+ GQES  S   R PC  CGR H G
Sbjct: 276 NRSGGQASRNVSYGSVFQRQSQRIPSQPIRSTVRSQPGQESIASTVRRIPCTSCGRNHWG 335

Query: 160 SCLVKVGECHYCKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTKQKG 207
            CLV  G C+ C++ GHF ++CP L   +Q +Q   SQ + Q + + V  E  S  +QKG
Sbjct: 336 QCLVGAGACYQCRQPGHFKKDCPQLNMTVQRDQGVGSQTVEQSRVSVVPTEGTSGARQKG 395

BLAST of PI0014916 vs. NCBI nr
Match: KAA0067811.1 (putative Gag-pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 100.9 bits (250), Expect = 1.3e-17
Identity = 71/186 (38.17%), Postives = 94/186 (50.54%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTME-----SVILKDGSNNNLHLVCRVNKILKLNLV 99
           AKW +FSQLVETA+RVEQ + + K  +E     S                +N   + +  
Sbjct: 216 AKWTNFSQLVETAIRVEQSITEEKSAVELSRGTSTASGFRGREQRRFTPGINISSRQDFK 275

Query: 100 N------SFSDNEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRFHRG 159
           N      S + + GSVF R S       ++    S+ GQES  S   R PC  CGR HRG
Sbjct: 276 NRSGGQASRNVSYGSVFQRQSQRIPSQPIRSTVRSQPGQESIASTVRRIPCTSCGRNHRG 335

Query: 160 SCLVKVGECHYCKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTKQKG 207
            CLV  G C+ C + GHF ++CP LK  +Q +Q   SQ + Q + + V  E  S  +QKG
Sbjct: 336 QCLVGAGVCYQCGQPGHFKKDCPQLKMTVQRDQGVGSQTIEQSRVSVVPTEGTSGARQKG 395

BLAST of PI0014916 vs. NCBI nr
Match: KAA0041696.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 100.5 bits (249), Expect = 1.8e-17
Identity = 70/189 (37.04%), Postives = 99/189 (52.38%), Query Frame = 0

Query: 40  AKWNDFSQLVETALRVEQGLLKTKPTMESVILKDGSNNNLHLVCRVNKILKLNL-VNSFS 99
           AKW +FSQLVETALRVEQ + + K  +E   L  G++       R  +   L + ++S  
Sbjct: 437 AKWTNFSQLVETALRVEQSITEEKSAVE---LSRGTSTASGFRGREERRFTLGINISSRQ 496

Query: 100 D-------------NEGSVFLRPS-------VKEVTMSRSGQESTTSNQDRSPCGYCGRF 159
           D             + GSVF R S       ++    S+ GQ+S  S+  ++PC  CGR 
Sbjct: 497 DFKNRSGGQASRNVSYGSVFQRQSQRIPSQPIRSTVRSQPGQKSIASSVKQTPCSSCGRN 556

Query: 160 HRGSCLVKVGECHYCKKLGHFMRECPLLKRELQENQKEESQLMNQQKTTPVGKEEPSDTK 207
           HRG CLV  G C+ C + GHF ++CP L   +Q +Q   SQ + Q + + V  E  S  +
Sbjct: 557 HRGQCLVGAGVCYQCGQPGHFKKDCPQLNMTVQRDQGVGSQTVEQSRVSVVPTEGTSGAR 616

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7UZA92.2e-1840.57Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5A7V2E02.2e-1839.15Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold10... [more]
A0A5A7VQD26.5e-1838.17Putative Gag-pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A5D3BTP38.5e-1836.51Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37... [more]
A0A5D3C7J18.5e-1837.04Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold59... [more]
Match NameE-valueIdentityDescription
KAA0060440.14.6e-1840.57retrotransposon protein [Cucumis melo var. makuwa][more]
KAA0059911.14.6e-1839.15reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0045363.16.0e-1838.71DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
KAA0067811.11.3e-1738.17putative Gag-pol protein [Cucumis melo var. makuwa][more]
KAA0041696.11.8e-1737.04DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 166..187
NoneNo IPR availableGENE3D4.10.60.10coord: 139..181
e-value: 2.4E-6
score: 29.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 191..206
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 171..206
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 176..190
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 149..165
e-value: 0.0056
score: 25.1
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 149..164
e-value: 8.9E-5
score: 22.4
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 150..164
score: 8.960441
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 145..170

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0014916.1PI0014916.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding