PI0014164 (gene) Melon (PI 482460) v1

BLAST of PI0014164 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.0e-43
Identity = 89/202 (44.06%), Postives = 132/202 (65.35%), Query Frame = 0

Query: 4   LNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHLTLA 63
           LNT+  I   L + F  LF + + S   R +  P   GAWP++GHL  L  ++  H  L 
Sbjct: 10  LNTI-AIASILSLIFLCLFLYRKNS---RGKDAPVVSGAWPILGHLSLLNGSQTPHKVLG 69

Query: 64  KMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAMMVF 123
            +AD YGP+FT + GMK AL++SNWE++KE FTTND   +SRPKLVA + ++Y+ A +  
Sbjct: 70  ALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGL 129

Query: 124 SPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAK--VVVE 183
           +PY P+WR +RKI T E L+N R++Q  +IR  EV+T +KEL+++W   NK      +V+
Sbjct: 130 APYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVD 189

Query: 184 MKRRLADITLNTIFKMVIGKRF 204
           +K+ LA +T N + +MV+GKR+
Sbjct: 190 IKQWLAYLTFNMVVRMVVGKRY 207

BLAST of PI0014164 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-40
Identity = 87/209 (41.63%), Postives = 131/209 (62.68%), Query Frame = 0

Query: 6   TLFGIIFPLLIFFYVLFTWS-RRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHLTLAK 65
           T  G++   LIF   LF +   + V    +  P  GGAWP+ GHL  L  +K  H  L  
Sbjct: 11  TTIGVV--SLIFLLCLFLYGPLKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGA 70

Query: 66  MADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAMMVFS 125
           +A+ +GP+FT + G KKAL+VS+WE+A+E FTTND   ++RPKL+ ++ + Y+ AM++ +
Sbjct: 71  LAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVA 130

Query: 126 PYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWR-LNNKGAKVVVEMK 185
           PY P+WR +RKI   E+L++ RV+QLQ +R  EV+  + ELY++WR   N+     VE+K
Sbjct: 131 PYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELK 190

Query: 186 RRLADITLNTIFKMVIGKRFSSMEYGNEK 213
           +  A    N + +MV+GKRF S    +EK
Sbjct: 191 QWFAQPIFNMVLRMVVGKRFLSATATDEK 217

BLAST of PI0014164 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-40
Identity = 89/197 (45.18%), Postives = 122/197 (61.93%), Query Frame = 0

Query: 7   LFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHLTLAKMA 66
           L G    L  FFY  F W + +   + + PP+  GAWP+IGHLH L      H  L+  A
Sbjct: 11  LLGFFVLLPCFFY--FVWKKPN--NKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFA 70

Query: 67  DAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAMMVFSPY 126
           D  GP+F  + G+ +AL+V+N EIAKE FTTND  F +RP  VA+K + Y+Y M+  +PY
Sbjct: 71  DKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPY 130

Query: 127 TPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVVEMKRRL 186
            P+WR +RKI  LE L+N R+  L+++   E+    KELY+LW   N     +V+MK+ L
Sbjct: 131 GPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDF-CLVDMKQWL 190

Query: 187 ADITLNTIFKMVIGKRF 204
           AD+TLN   KMV+GKRF
Sbjct: 191 ADLTLNMSVKMVVGKRF 202

BLAST of PI0014164 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.1e-40
Identity = 83/199 (41.71%), Postives = 126/199 (63.32%), Query Frame = 0

Query: 6   TLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKP-THLTLAK 65
           +LF +  P+L+F ++     ++S   +    P   GAWP+IGHLH L   +   + TL K
Sbjct: 4   SLFSLFVPILVFVFIAL--FKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGK 63

Query: 66  MADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAMMVFS 125
           MAD YGP  + + G  +A +VS++E+AK+ FT ND   ASRP   A+K + Y++A+  F+
Sbjct: 64  MADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFA 123

Query: 126 PYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVVEMKR 185
           PY+  WR +RKIAT+ELL+N R+  L+++R  E+   +K+LY LW  N     V+V++K 
Sbjct: 124 PYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKS 183

Query: 186 RLADITLNTIFKMVIGKRF 204
            L D+TLN I +MV GKR+
Sbjct: 184 WLEDMTLNMIVRMVAGKRY 200

BLAST of PI0014164 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 164.5 bits (415), Expect = 1.3e-39
Identity = 82/199 (41.21%), Postives = 123/199 (61.81%), Query Frame = 0

Query: 6   TLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKP-THLTLAK 65
           +LF +  P+L+F ++     ++S   +    P   GAWP+IGHLH L   +   + TL K
Sbjct: 4   SLFSLFVPILVFVFIAL--FKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGK 63

Query: 66  MADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAMMVFS 125
           MAD YGP  + R G  +  +VS++E+AK+ FT ND   ASRP   A+K + YD A+  F+
Sbjct: 64  MADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFA 123

Query: 126 PYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVVEMKR 185
           PY+  WR +RKIATLELL+N R+  L+++R  E+   M++LY LW        V+V++K 
Sbjct: 124 PYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKS 183

Query: 186 RLADITLNTIFKMVIGKRF 204
            L D++LN + +MV GKR+
Sbjct: 184 WLEDMSLNMMVRMVAGKRY 200

BLAST of PI0014164 vs. ExPASy TrEMBL
Match: A0A5A7SQD8 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00980 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.3e-106
Identity = 191/213 (89.67%), Postives = 201/213 (94.37%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           M QLNTLFGIIFPLLI FYVLFTWSRRSVAQRKRLPP+AGGAWPVIGHLH L  ++PTH+
Sbjct: 1   MGQLNTLFGIIFPLLILFYVLFTWSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
           T AKMADAYGPMFTFRFGM +ALIVSNWE+AKEFFTTND IFASRPKLVASK LAYDYAM
Sbjct: 61  TFAKMADAYGPMFTFRFGMNEALIVSNWELAKEFFTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVV 180
           M FSPY+PHWRHVRKIATLELLTNHRV QLQYIRAFE+KTWMKELYELWRLNNKG KVVV
Sbjct: 121 MGFSPYSPHWRHVRKIATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVVV 180

Query: 181 EMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           EMK+RLADITLNTIFKMVIGKRFSS+EYGNEKF
Sbjct: 181 EMKKRLADITLNTIFKMVIGKRFSSIEYGNEKF 213

BLAST of PI0014164 vs. ExPASy TrEMBL
Match: A0A1S3CMD6 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502577 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.3e-106
Identity = 191/213 (89.67%), Postives = 201/213 (94.37%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           M QLNTLFGIIFPLLI FYVLFTWSRRSVAQRKRLPP+AGGAWPVIGHLH L  ++PTH+
Sbjct: 1   MGQLNTLFGIIFPLLILFYVLFTWSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
           T AKMADAYGPMFTFRFGM +ALIVSNWE+AKEFFTTND IFASRPKLVASK LAYDYAM
Sbjct: 61  TFAKMADAYGPMFTFRFGMNEALIVSNWELAKEFFTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVV 180
           M FSPY+PHWRHVRKIATLELLTNHRV QLQYIRAFE+KTWMKELYELWRLNNKG KVVV
Sbjct: 121 MGFSPYSPHWRHVRKIATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVVV 180

Query: 181 EMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           EMK+RLADITLNTIFKMVIGKRFSS+EYGNEKF
Sbjct: 181 EMKKRLADITLNTIFKMVIGKRFSSIEYGNEKF 213

BLAST of PI0014164 vs. ExPASy TrEMBL
Match: A0A0A0LIS1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G853140 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 9.5e-105
Identity = 187/213 (87.79%), Postives = 203/213 (95.31%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           ME LNTLFGIIFPLLIFFY+LFT SRRSVAQRKRLPP+AGGAWPVIGHLH L  ++PTH+
Sbjct: 1   MEYLNTLFGIIFPLLIFFYLLFTCSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
           TLAKMADAYGPMFTFRFGMK+ALIVSNW++AKE FTTND IFASRPKLVASK LAYDYAM
Sbjct: 61  TLAKMADAYGPMFTFRFGMKRALIVSNWDLAKEIFTTNDRIFASRPKLVASKILAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVV 180
           M FSPY+PHWR+VRKIATLELLTNHRVDQLQYIRAFEV+TWM+ELYELWRLNNKG KVVV
Sbjct: 121 MGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMEELYELWRLNNKGEKVVV 180

Query: 181 EMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           EMK+RLAD+TLNT+FKMVIGK+FSSMEYGNEKF
Sbjct: 181 EMKKRLADVTLNTMFKMVIGKKFSSMEYGNEKF 213

BLAST of PI0014164 vs. ExPASy TrEMBL
Match: A0A6J1IFL6 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.8e-87
Identity = 165/214 (77.10%), Postives = 182/214 (85.05%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           ME L  L GIIFP L F YVLFT SRRSVA RKR+PPEAGGAWPVIGHLH L    PTH+
Sbjct: 1   MEHLYALAGIIFPFLFFVYVLFTSSRRSVAHRKRIPPEAGGAWPVIGHLHLLHAGDPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
            LAKMADAYGPMFT RFGMKKAL+VS+WEIAKEF TTND IFASRPKLVASK LAYDYAM
Sbjct: 61  ALAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYEL-WRLNNKGAKVV 180
           M FSPYTPHWR VRKIATLELLTNHR++QLQ+IR FEV+ WMKEL+++  R N +G KV 
Sbjct: 121 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRVFEVQRWMKELHKMSIRSNERGDKVA 180

Query: 181 VEMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           VEMK+ LADITLNTIFKMVIGKRFS++++G E F
Sbjct: 181 VEMKKWLADITLNTIFKMVIGKRFSAIQHGTENF 214

BLAST of PI0014164 vs. ExPASy TrEMBL
Match: A0A6J1F5K6 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442570 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.8e-87
Identity = 164/214 (76.64%), Postives = 183/214 (85.51%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           ME L  L GIIFP L F YVLFT SRRSVA RKR+PPEAGGAWP+IGHLH L    PTH+
Sbjct: 1   MEHLYALAGIIFPFLFFLYVLFTSSRRSVAHRKRIPPEAGGAWPLIGHLHLLHAGDPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
            LAKMADAYGPMFT RFGMKKAL+VS+WEIAKEF TTND IFASRPKLVASK LAYDYAM
Sbjct: 61  VLAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYEL-WRLNNKGAKVV 180
           M FSPYTPHWR VRKIATLELLTNHR++QLQ+IRAFEV+ WMKEL+++  + N +G KV 
Sbjct: 121 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQRWMKELHKMSIKSNERGEKVA 180

Query: 181 VEMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           VEMK+ LADITLNTIFKMVIGKRFS++++G E F
Sbjct: 181 VEMKKWLADITLNTIFKMVIGKRFSAIQHGTENF 214

BLAST of PI0014164 vs. NCBI nr
Match: XP_008464769.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo])

HSP 1 Score: 395.6 bits (1015), Expect = 2.7e-106
Identity = 191/213 (89.67%), Postives = 201/213 (94.37%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           M QLNTLFGIIFPLLI FYVLFTWSRRSVAQRKRLPP+AGGAWPVIGHLH L  ++PTH+
Sbjct: 1   MGQLNTLFGIIFPLLILFYVLFTWSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
           T AKMADAYGPMFTFRFGM +ALIVSNWE+AKEFFTTND IFASRPKLVASK LAYDYAM
Sbjct: 61  TFAKMADAYGPMFTFRFGMNEALIVSNWELAKEFFTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVV 180
           M FSPY+PHWRHVRKIATLELLTNHRV QLQYIRAFE+KTWMKELYELWRLNNKG KVVV
Sbjct: 121 MGFSPYSPHWRHVRKIATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVVV 180

Query: 181 EMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           EMK+RLADITLNTIFKMVIGKRFSS+EYGNEKF
Sbjct: 181 EMKKRLADITLNTIFKMVIGKRFSSIEYGNEKF 213

BLAST of PI0014164 vs. NCBI nr
Match: KAA0032758.1 (cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98789.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 395.6 bits (1015), Expect = 2.7e-106
Identity = 191/213 (89.67%), Postives = 201/213 (94.37%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           M QLNTLFGIIFPLLI FYVLFTWSRRSVAQRKRLPP+AGGAWPVIGHLH L  ++PTH+
Sbjct: 1   MGQLNTLFGIIFPLLILFYVLFTWSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
           T AKMADAYGPMFTFRFGM +ALIVSNWE+AKEFFTTND IFASRPKLVASK LAYDYAM
Sbjct: 61  TFAKMADAYGPMFTFRFGMNEALIVSNWELAKEFFTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVV 180
           M FSPY+PHWRHVRKIATLELLTNHRV QLQYIRAFE+KTWMKELYELWRLNNKG KVVV
Sbjct: 121 MGFSPYSPHWRHVRKIATLELLTNHRVAQLQYIRAFEIKTWMKELYELWRLNNKGEKVVV 180

Query: 181 EMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           EMK+RLADITLNTIFKMVIGKRFSS+EYGNEKF
Sbjct: 181 EMKKRLADITLNTIFKMVIGKRFSSIEYGNEKF 213

BLAST of PI0014164 vs. NCBI nr
Match: XP_011652397.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN60056.2 hypothetical protein Csa_002536 [Cucumis sativus])

HSP 1 Score: 389.4 bits (999), Expect = 2.0e-104
Identity = 187/213 (87.79%), Postives = 203/213 (95.31%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           ME LNTLFGIIFPLLIFFY+LFT SRRSVAQRKRLPP+AGGAWPVIGHLH L  ++PTH+
Sbjct: 1   MEYLNTLFGIIFPLLIFFYLLFTCSRRSVAQRKRLPPKAGGAWPVIGHLHLLNASEPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
           TLAKMADAYGPMFTFRFGMK+ALIVSNW++AKE FTTND IFASRPKLVASK LAYDYAM
Sbjct: 61  TLAKMADAYGPMFTFRFGMKRALIVSNWDLAKEIFTTNDRIFASRPKLVASKILAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVV 180
           M FSPY+PHWR+VRKIATLELLTNHRVDQLQYIRAFEV+TWM+ELYELWRLNNKG KVVV
Sbjct: 121 MGFSPYSPHWRYVRKIATLELLTNHRVDQLQYIRAFEVETWMEELYELWRLNNKGEKVVV 180

Query: 181 EMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           EMK+RLAD+TLNT+FKMVIGK+FSSMEYGNEKF
Sbjct: 181 EMKKRLADVTLNTMFKMVIGKKFSSMEYGNEKF 213

BLAST of PI0014164 vs. NCBI nr
Match: XP_023535449.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 343.2 bits (879), Expect = 1.6e-90
Identity = 167/214 (78.04%), Postives = 188/214 (87.85%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           ME L  L GIIFP L+F YV FTWSRRSVA RKR+PPEAGGAWPVIGHLH L   +PTH+
Sbjct: 1   MEHLRPLAGIIFPFLLFLYVFFTWSRRSVAHRKRIPPEAGGAWPVIGHLHLLHAGEPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
           TLAKMADAYGP+FTFRFGMKKALIVS+WEIAKEFFTTND IFASRPKLVASK LAYDYAM
Sbjct: 61  TLAKMADAYGPIFTFRFGMKKALIVSSWEIAKEFFTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYEL-WRLNNKGAKVV 180
           + FSPYTPHWR VRKIATLELLTNHR++QLQ+IRAFEV+ WM EL+++  + N +G KV 
Sbjct: 121 IGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQQWMNELHKMSIKSNERGEKVA 180

Query: 181 VEMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           VEMK+ LADITLNTIFKMVIGKRFS++++GNE F
Sbjct: 181 VEMKKWLADITLNTIFKMVIGKRFSAIQHGNENF 214

BLAST of PI0014164 vs. NCBI nr
Match: XP_022976392.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])

HSP 1 Score: 332.0 bits (850), Expect = 3.7e-87
Identity = 165/214 (77.10%), Postives = 182/214 (85.05%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           ME L  L GIIFP L F YVLFT SRRSVA RKR+PPEAGGAWPVIGHLH L    PTH+
Sbjct: 1   MEHLYALAGIIFPFLFFVYVLFTSSRRSVAHRKRIPPEAGGAWPVIGHLHLLHAGDPTHI 60

Query: 61  TLAKMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAM 120
            LAKMADAYGPMFT RFGMKKAL+VS+WEIAKEF TTND IFASRPKLVASK LAYDYAM
Sbjct: 61  ALAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 120

Query: 121 MVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYEL-WRLNNKGAKVV 180
           M FSPYTPHWR VRKIATLELLTNHR++QLQ+IR FEV+ WMKEL+++  R N +G KV 
Sbjct: 121 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRVFEVQRWMKELHKMSIRSNERGDKVA 180

Query: 181 VEMKRRLADITLNTIFKMVIGKRFSSMEYGNEKF 214
           VEMK+ LADITLNTIFKMVIGKRFS++++G E F
Sbjct: 181 VEMKKWLADITLNTIFKMVIGKRFSAIQHGTENF 214

BLAST of PI0014164 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 167.2 bits (422), Expect = 1.5e-41
Identity = 83/199 (41.71%), Postives = 126/199 (63.32%), Query Frame = 0

Query: 6   TLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKP-THLTLAK 65
           +LF +  P+L+F ++     ++S   +    P   GAWP+IGHLH L   +   + TL K
Sbjct: 4   SLFSLFVPILVFVFIAL--FKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGK 63

Query: 66  MADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAMMVFS 125
           MAD YGP  + + G  +A +VS++E+AK+ FT ND   ASRP   A+K + Y++A+  F+
Sbjct: 64  MADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFA 123

Query: 126 PYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVVEMKR 185
           PY+  WR +RKIAT+ELL+N R+  L+++R  E+   +K+LY LW  N     V+V++K 
Sbjct: 124 PYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKS 183

Query: 186 RLADITLNTIFKMVIGKRF 204
            L D+TLN I +MV GKR+
Sbjct: 184 WLEDMTLNMIVRMVAGKRY 200

BLAST of PI0014164 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 164.5 bits (415), Expect = 9.6e-41
Identity = 82/199 (41.21%), Postives = 123/199 (61.81%), Query Frame = 0

Query: 6   TLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKP-THLTLAK 65
           +LF +  P+L+F ++     ++S   +    P   GAWP+IGHLH L   +   + TL K
Sbjct: 4   SLFSLFVPILVFVFIAL--FKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGK 63

Query: 66  MADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAMMVFS 125
           MAD YGP  + R G  +  +VS++E+AK+ FT ND   ASRP   A+K + YD A+  F+
Sbjct: 64  MADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFA 123

Query: 126 PYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKVVVEMKR 185
           PY+  WR +RKIATLELL+N R+  L+++R  E+   M++LY LW        V+V++K 
Sbjct: 124 PYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKS 183

Query: 186 RLADITLNTIFKMVIGKRF 204
            L D++LN + +MV GKR+
Sbjct: 184 WLEDMSLNMMVRMVAGKRY 200

BLAST of PI0014164 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 142.9 bits (359), Expect = 3.0e-34
Identity = 81/201 (40.30%), Postives = 111/201 (55.22%), Query Frame = 0

Query: 4   LNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHLTLA 63
           L+ LF  IFP+LI        SR     +K   P   GAWP++GHLH      PTH+T  
Sbjct: 9   LSALF--IFPVLILIK-----SRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFG 68

Query: 64  KMADAYGPMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDYAMMVF 123
            MAD YGP+F  + G  K +I+++ E+AKE +T +D +   RP+L ASK L Y+ + + F
Sbjct: 69  AMADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTF 128

Query: 124 SPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAK-VVVEM 183
           SPY  +WR +RKIA  EL +   VD     RA E     + LY  W    K  + V+V+M
Sbjct: 129 SPYGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDM 188

Query: 184 KRRLADITLNTIFKMVIGKRF 204
           K+   D+T N    MV GKR+
Sbjct: 189 KQEFIDLTANISLMMVSGKRY 201

BLAST of PI0014164 vs. TAIR 10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )

HSP 1 Score: 131.7 bits (330), Expect = 6.9e-31
Identity = 71/181 (39.23%), Postives = 113/181 (62.43%), Query Frame = 0

Query: 33  KRLPPEAGGAWPVIGHLHHLKVNKP-THLTLAKMADAYGPMFTFRFGMKKALIVSNWEIA 92
           K LPP     +P+IGHLH LK  KP  H TL+ ++ + GP+F+ R G + A+I+S+   A
Sbjct: 28  KNLPPSPNICFPIIGHLHLLK--KPLLHRTLSHLSHSLGPVFSLRLGSRLAVIISSPTAA 87

Query: 93  KE-FFTTNDSIFASRPKLVASKFLAYDYAMMVFSPYTPHWRHVRKIATLELLTNHRVDQL 152
           +E F T ND + A+RP+ +  K++AYDY  MV +PY  HWR++R+I  LE+ + +R++  
Sbjct: 88  EECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWRNLRRITALEVFSTNRLNAS 147

Query: 153 QYIRAFEVKTWMKELYELWRLNNKGAKVVVEMKRRLADITLNTIFKMVIGKRFSSMEYGN 212
             IR  EVK  +++L++L    +      VE+++ L  +TLN I +M+ GKRF   + G 
Sbjct: 148 AEIRHDEVKMLLQKLHDL----SVERPAKVELRQLLTGLTLNVIMRMMTGKRFFEEDDGG 202

BLAST of PI0014164 vs. TAIR 10
Match: AT2G23220.1 (cytochrome P450, family 81, subfamily D, polypeptide 6 )

HSP 1 Score: 128.3 bits (321), Expect = 7.6e-30
Identity = 74/205 (36.10%), Postives = 117/205 (57.07%), Query Frame = 0

Query: 1   MEQLNTLFGIIFPLLIFFYVLFTWSRRSVAQRKRLPPEAGGAWPVIGHLHHLKVNKPTHL 60
           ME    L  + F L I   +LF   +R    +  LPP      P+IGHLH LK+  P H 
Sbjct: 16  METYFLLLSLAFFLFISVKLLF--GKR--LSKFNLPPSPARPLPLIGHLHLLKL--PLHR 75

Query: 61  TLAKMADAYG--PMFTFRFGMKKALIVSNWEIAKEFFTTNDSIFASRPKLVASKFLAYDY 120
           T    + + G  P+F  R G +  ++VS++ IA+E FT ND +FA+RP+L+  K + Y+ 
Sbjct: 76  TFLSFSQSLGGAPIFCLRLGNRLTVVVSSYSIAEECFTKNDIVFANRPELILGKHIEYNS 135

Query: 121 AMMVFSPYTPHWRHVRKIATLELLTNHRVDQLQYIRAFEVKTWMKELYELWRLNNKGAKV 180
             M  +PY  HWR++R+IATLE+  +H+++    +R  E++  +  L +    N++    
Sbjct: 136 TTMTSAPYGDHWRNLRRIATLEIFASHKLNGFLSVRKDEIRQLLLRLSK----NSRHGFA 195

Query: 181 VVEMKRRLADITLNTIFKMVIGKRF 204
            VEM+  L ++T+N +F+MV GKRF
Sbjct: 196 KVEMRHLLFELTINNVFRMVAGKRF 210