PI0014103 (gene) Melon (PI 482460) v1

Overview
NamePI0014103
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionmitotic checkpoint serine/threonine-protein kinase BUB1
Locationchr01: 2459888 .. 2464054 (-)
RNA-Seq ExpressionPI0014103
SyntenyPI0014103
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGAAAGCTACAAGCAATGACGATTTTCTTCGCCGATTCTCAAATCTCCACCACCACAGCATCCGCTCAAGACCCTTTGTTGCCTTGGCTCTGGTATTCCTTGATTTCTGTTATGTGATAGAGCTTATTTACAGCCATTCTTACACTCAATCTAAGGAAAATTGTTCGTGCTATTTGGTTCCATTTGCAGGTCCATCAAGGATGCTCTTGAAAATTCAACTTCAGGAAATGTTTCTGGGTCAGAACTCGCCAAGCTTCTCTCCGATTGCATTGGAAATTTCAGCGGCAATGTCAAGTACAAAAACGATGTTCGATTTCTCAAGATTTGGTTTCTCTCTGTGCGTAATTATCTTTCTTTAACTGTTATATTTGTGGGTTTAACTTATCGTCTTTGTTTCATACATGCGGTTGATTAGATAACTTGTATGTGAGCATGTGCTATGTTTTTTATGAAGTATCTTTTGAAATTTGCTTATGCTACCTACTTCCCTACCCTTCTCTTTCCTTTTCCAATCATGCTCACTTTAGATTATTTGTGGGTTTATTCGGGTTCTTAATAAATTGATTCTTCCGTTGGTTATTATATTTGTATGATTTTGTTTTATGTGTAAATGCTTGGATCTCTTTGAAAAGTTGGACATTTTCGACAATTTTTAATCCGGTTATAACATTATGTTGTTCGTGCAAATAGATTGGTATGAGGGAGGATTTTGAAACTGGGTTCAAGGAATTGTTGGAGCAGGAGATATGCATTGGCAATTCTTTACTTTATATTTGGTTGGCAGCGTTTCTCGAATCCAAGGGGAGGTTGAACGACGCTAACATAGTTTATGAGCTTGGGTTGATGAGGTATTCCTATCTCTCTCTCTCTCATTGACATGCTGTTAAATACATGTAGATTATGCCTGTGAAAATTTTTGTTGATTAGGATTTTTTGTTTGGTTGTGTTCTGTGTTGTTTAGTTCCTTACATTTTTAAGTGATAAACTGACAATAGGAACGCCGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAAGATTGTTTGTTGATAGGATATCTGAGTTGGTCGATACTCATTCAATGCAAGTGGTACGTGATCTTAAAGCATAAAACCATTGAAGTTCACATTCCTTCAATTGTATAATTTAAGAACTTTGACAAGGTTGGTTTTCATTTTTGTAGAATGATGTCAGTGAATCCACTAAATTTGCAGAAAGTTACATCAACCCTTGGTCTTCCTCCATTATGAACGATTTATTAAACAAGATTAATCCAAAGATTATGAAGTATGATGTAAGAAATTACAGTTTTCTTTCGCCAAATGATGAAAGCAAGCTGGTTATATTGTTCAAATAAATTACTCTCAAACTAACTTTAATATGAATTTAATGTGTGTTTCAGGGTTACCATTCAAGTACCAAAGCCTACTCTAAAAAGATGAATCTAAAGTCATTGCGCAATTCATCAAGAAATAAGACTATTGAGATTGGTAATTTTTTATGTTACATTATAGTTAGATTTGTCACCTAAACGGATAGAACTACCTTCAGTCTGTCCTTCAAAAGAACGAGCTTTCTTGAAATATTGTATAAGAAATGGAAGAAAAAGTTTTCTTATGCCACGGATTCATTTTTCATTATAATGATACCCTTACCAACATTTACAGGTGGAAAGAAGTATGAGATTAAAGGCTGTGCCGGTACAGGCGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCAGATGATGTAGTAGCATTGAAGGTAAAATCTCTTATTGATTGACGTGCAATCTGCATATGTCCTTTATTTCCAGCATTCCTTATATGTCATAACTGAATGTGAACTAGTTGGATATCATGATTCTCTTCAAATGCTTGCCAGATACAAACACCTCCTTTTCCTTGGGAGTTCTACATGTATCGTCAACTCGATCTACGTGTTGATGCGAAGGAAGTATGTACTAGCCCCTTATTTTCATCAAGCATTTATTACCTCTAAATGTTAGCAACTCGATTTATACTCCTACTCCATCTTTCATAATGTCAGAGGTCAAGTTTTGGTTGTGCTCATGCCATGTATCTCTATTCTGACTACAGCATACTTACATGTGATTACTTGGCTCATGGAACATTACAGGTTAGTATACAGATTCTGAACCTTTTATACTGTATGACCTTTTAAAATATTCATTGTAAGTAACTATCTTGTTAAAACCTCGAGCAGGATGCCATAAATTCGTATGTAGTCCTTGGAAAGACAATGGAAGAAGTTTTATGTATTTATTACACCATAGAGATGCTCTACATGCTAGAAACTTTACACAGCATTGGAATTATCCATGGGGATTTTAAACCGGACAATCTGCTTATTCGTTATGCGGGGTAAGTATATTTTGGTTCTAGATCTTTATTGATGACTTCCATCTTTCTGAGAGTTGATTTGATTTATATAGTTCCCGATTACAATACTCGTGAAAAAAGAAAAGAACCGTAATAAATGAAAAGATGAGACCTCTAGTTGTTATATACAACTTCAGCCTACCAACAATGATTAGCACCCGACTGGAGAATGTACTCCAATTCAATAGTTCTTTACCAATTTATTTTATTTAACCGTTATGCAGGGAAAGCCTAACAGAAGATGAATTTCAGGAACGTCGTGGTCCTTGGCAAGATCAGGTATGTAGTATCATCCCCTTTTTTGTTCCCAAGTCATTACATGTCTACTTGGAATGGCCTTTTAAGTGCTGAAAAACAGTGTTTTTAAATGAAAAAAATTGCGTTTTAAGCACTTTTAAAGGCATTCCAAACCGACCCTATTAACTGTGAGCAAACAAGATTATTTGTAGTTTTTATATTATAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCAACTCTTTCCTGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCCGGCTTTCGTTGTCCAGAAATGCTAGAGAATAGACCTTGGAAATATCAGGTCAGTTTCTTCTGCAATGTGGTTGTTTATAACAGCTCTAGAGAAAACCCACTGTAAAGAAGAAAACAGAAGCTCTACCAATAGCATTTTGTCGTTTGTACAGGTAGACATCTATGGCTTTTGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAGGCATCATCTGATGGTGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTAGTTCCCATTTCTGGCTTTTTAGGTCCCATTAAGTAATCATTTTGTTTTTTGTTTCTAAAAATAAAGCGTATTTCCTTTTAATTTTTTATAATAATTTTCATCTGTCTTAGGAACAAAAGTTTAATTTTTAGCCAAATTCTAAAAACAAAAACTAAAATTTTAAGAATTGTTCTTTTTAGTTTTCAAATTTTGGCTAATTTTTGAAAGTATTGGTTAAAAGTAGATAAAAAATTAAGAAATTTAAAGGTGAAAGGAGTGTTGTTAAGCTTAATTTTCAAAAACAGAAAACAAAATACCAAATAGTTACCAAACGGGACGATAATCGTCTGTTTTCTGTTTTTTCCCATCAATATGTTGTGCCTGAATTTTAACTCTTCTAGATTCTTCGTAGAGTTTTAGATCAACAACTTTGTTTTATAGAGTGCAACTGCCCTCTCACCTCCTCCCCTCCTCCCCCTGACTTGTTCTCTCTGTTCCTCTATAGGTATTGGAACATTGAATTATGGAAGAATCTGTTCACGAAGTTGCTTAATATCACACCAGGTCATTACAACAAAGAGTTGTTGCAGAGTTTAAGGAAATCTTTCCAGGATTACATGTGCTCCAATCCTCAACTTATAAGGAAGTTAAAGGAATTACTTGTAAAACAAAGAGCCTCCTTATGTACTTCTTAATCAAGAACCTCCTCTTGAATATATGTAAATTCCCCAAAAAATGTAGGTGGTGGTTCATGTCTGCCTCTAGCAAACTTAGATTAGTTCATTGCTCTGATGATGGAATGTCTGTATTCTTTTTTCTTGTCTGTATTATTAAAAACAAAGAAAGTTTGAAGAGTTGTCGTAGTCTGAGTGGTTTCTTTGAAATGCTGTCTTGTGGGGTTCATTGTTTATCCTAGAGGTAAGCTGTAATGTCGTAGGTTGCAGAGATTCATT

mRNA sequence

CAGAAAGCTACAAGCAATGACGATTTTCTTCGCCGATTCTCAAATCTCCACCACCACAGCATCCGCTCAAGACCCTTTGTTGCCTTGGCTCTGGTCCATCAAGGATGCTCTTGAAAATTCAACTTCAGGAAATGTTTCTGGGTCAGAACTCGCCAAGCTTCTCTCCGATTGCATTGGAAATTTCAGCGGCAATGTCAAGTACAAAAACGATGTTCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACTGGGTTCAAGGAATTGTTGGAGCAGGAGATATGCATTGGCAATTCTTTACTTTATATTTGGTTGGCAGCGTTTCTCGAATCCAAGGGGAGGTTGAACGACGCTAACATAGTTTATGAGCTTGGGTTGATGAGGAACGCCGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAAGATTGTTTGTTGATAGGATATCTGAGTTGGTCGATACTCATTCAATGCAAGTGAATGATGTCAGTGAATCCACTAAATTTGCAGAAAGTTACATCAACCCTTGGTCTTCCTCCATTATGAACGATTTATTAAACAAGATTAATCCAAAGATTATGAAGTATGATGGTTACCATTCAAGTACCAAAGCCTACTCTAAAAAGATGAATCTAAAGTCATTGCGCAATTCATCAAGAAATAAGACTATTGAGATTGGTGGAAAGAAGTATGAGATTAAAGGCTGTGCCGGTACAGGCGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCAGATGATGTAGTAGCATTGAAGATACAAACACCTCCTTTTCCTTGGGAGTTCTACATGTATCGTCAACTCGATCTACGTGTTGATGCGAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGCCATGTATCTCTATTCTGACTACAGCATACTTACATGTGATTACTTGGCTCATGGAACATTACAGGATGCCATAAATTCGTATGTAGTCCTTGGAAAGACAATGGAAGAAGTTTTATGTATTTATTACACCATAGAGATGCTCTACATGCTAGAAACTTTACACAGCATTGGAATTATCCATGGGGATTTTAAACCGGACAATCTGCTTATTCGTTATGCGGGGGAAAGCCTAACAGAAGATGAATTTCAGGAACGTCGTGGTCCTTGGCAAGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCAACTCTTTCCTGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCCGGCTTTCGTTGTCCAGAAATGCTAGAGAATAGACCTTGGAAATATCAGGTAGACATCTATGGCTTTTGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAGGCATCATCTGATGGTGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTGGAACATTGAATTATGGAAGAATCTGTTCACGAAGTTGCTTAATATCACACCAGGTCATTACAACAAAGAGTTGTTGCAGAGTTTAAGGAAATCTTTCCAGGATTACATGTGCTCCAATCCTCAACTTATAAGGAAGTTAAAGGAATTACTTGTAAAACAAAGAGCCTCCTTATGTACTTCTTAATCAAGAACCTCCTCTTGAATATATGTAAATTCCCCAAAAAATGTAGGTGGTGGTTCATGTCTGCCTCTAGCAAACTTAGATTAGTTCATTGCTCTGATGATGGAATGTCTGTATTCTTTTTTCTTGTCTGTATTATTAAAAACAAAGAAAGTTTGAAGAGTTGTCGTAGTCTGAGTGGTTTCTTTGAAATGCTGTCTTGTGGGGTTCATTGTTTATCCTAGAGGTAAGCTGTAATGTCGTAGGTTGCAGAGATTCATT

Coding sequence (CDS)

ATGACGATTTTCTTCGCCGATTCTCAAATCTCCACCACCACAGCATCCGCTCAAGACCCTTTGTTGCCTTGGCTCTGGTCCATCAAGGATGCTCTTGAAAATTCAACTTCAGGAAATGTTTCTGGGTCAGAACTCGCCAAGCTTCTCTCCGATTGCATTGGAAATTTCAGCGGCAATGTCAAGTACAAAAACGATGTTCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACTGGGTTCAAGGAATTGTTGGAGCAGGAGATATGCATTGGCAATTCTTTACTTTATATTTGGTTGGCAGCGTTTCTCGAATCCAAGGGGAGGTTGAACGACGCTAACATAGTTTATGAGCTTGGGTTGATGAGGAACGCCGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAAGATTGTTTGTTGATAGGATATCTGAGTTGGTCGATACTCATTCAATGCAAGTGAATGATGTCAGTGAATCCACTAAATTTGCAGAAAGTTACATCAACCCTTGGTCTTCCTCCATTATGAACGATTTATTAAACAAGATTAATCCAAAGATTATGAAGTATGATGGTTACCATTCAAGTACCAAAGCCTACTCTAAAAAGATGAATCTAAAGTCATTGCGCAATTCATCAAGAAATAAGACTATTGAGATTGGTGGAAAGAAGTATGAGATTAAAGGCTGTGCCGGTACAGGCGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCAGATGATGTAGTAGCATTGAAGATACAAACACCTCCTTTTCCTTGGGAGTTCTACATGTATCGTCAACTCGATCTACGTGTTGATGCGAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGCCATGTATCTCTATTCTGACTACAGCATACTTACATGTGATTACTTGGCTCATGGAACATTACAGGATGCCATAAATTCGTATGTAGTCCTTGGAAAGACAATGGAAGAAGTTTTATGTATTTATTACACCATAGAGATGCTCTACATGCTAGAAACTTTACACAGCATTGGAATTATCCATGGGGATTTTAAACCGGACAATCTGCTTATTCGTTATGCGGGGGAAAGCCTAACAGAAGATGAATTTCAGGAACGTCGTGGTCCTTGGCAAGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCAACTCTTTCCTGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCCGGCTTTCGTTGTCCAGAAATGCTAGAGAATAGACCTTGGAAATATCAGGTAGACATCTATGGCTTTTGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAGGCATCATCTGATGGTGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTGGAACATTGAATTATGGAAGAATCTGTTCACGAAGTTGCTTAATATCACACCAGGTCATTACAACAAAGAGTTGTTGCAGAGTTTAAGGAAATCTTTCCAGGATTACATGTGCTCCAATCCTCAACTTATAAGGAAGTTAAAGGAATTACTTGTAAAACAAAGAGCCTCCTTATGTACTTCTTAA

Protein sequence

MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYSILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Homology
[Close]
BLAST of PI0014103 vs. ExPASy Swiss-Prot
Match: F4IVI0 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana OX=3702 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 3.1e-194
Identity = 316/518 (61.00%), Postives = 400/518 (77.22%), Query Frame = 0 

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLS 75
           +A DPL PWL  IK+++E+  +G  SG +L KLL DCI  +  + +Y+ND+RFLKIWFL 
Sbjct: 8   AAGDPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLY 67

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +  REDFE  ++E+ E EIC G+SLLY W A FLE KG    AN VY+ GL R AEP + 
Sbjct: 68  LEGREDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDR 127

Query: 136 LKKAKRLFVDRISELVDTHSMQ-VNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKY 195
           LK+A  LF+ RIS+     S++ V D +++T     ++NPW +S +N L++KI P+++KY
Sbjct: 128 LKEAHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKY 187

Query: 196 DGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDD 255
           DGYH S K +  K NL SL+N SRNK IEIGG+KY++KGCAG GGFAQV+K+++DSNPD+
Sbjct: 188 DGYHVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDE 247

Query: 256 VVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYSILTCDYLAHGTLQD 315
           VVALK+Q PPFPWEF+MYRQLD R+   +RSSFG A  +++YSDYSIL CDYL+HGTLQD
Sbjct: 248 VVALKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQD 307

Query: 316 AINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGESLTEDEF 375
            INSYVV+GK+MEEVLC+YYTIEML MLETLHS+GIIHGDFKPDNLLIRY  E+LTE  F
Sbjct: 308 VINSYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGF 367

Query: 376 QERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKYQVDIYG 435
            E+ G W  +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E++PWK+QVD YG
Sbjct: 368 HEKTGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYG 427

Query: 436 FCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGHYNK 495
            CV+VH MLH  YME+EKK S DG ++ +P++SFKRYW ++LWK LFTKLLN      + 
Sbjct: 428 LCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDT 487

Query: 496 ELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           E L++LRKS ++Y+CS+P+L++KL ELL KQR SLC+S
Sbjct: 488 ETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

[Close]
BLAST of PI0014103 vs. ExPASy Swiss-Prot
Match: P41695 (Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BUB1 PE=1 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 2.6e-47
Identity = 107/327 (32.72%), Postives = 176/327 (53.82%), Query Frame = 0 

Query: 173 NPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKT------IEIGG 232
           NP S+++    L++I+P + +Y+ +++  +       LK +   SRN+        +  G
Sbjct: 643 NPLSNNLRAKFLSEISPPLFQYNTFYNYNQELKMSSLLKKIHRVSRNENKNPIVDFKKTG 702

Query: 233 KKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV-DAKERS 292
             Y I+G  G GG+A VY +  +S+   + ALK++ P   WE+Y+  Q++ R+  +    
Sbjct: 703 DLYCIRGELGEGGYATVYLA--ESSQGHLRALKVEKPASVWEYYIMSQVEFRLRKSTILK 762

Query: 293 SFGCAHAMYLYSDYSILTCDYLAHGTLQDAIN----SYVVLGKTMEEVLCIYYTIEMLYM 352
           S   A A++L+ D S L  +Y + GT+ D IN      +     M+E LC++ T+E++ +
Sbjct: 763 SIINASALHLFLDESYLVLNYASQGTVLDLINLQREKAIDGNGIMDEYLCMFITVELMKV 822

Query: 353 LETLHSIGIIHGDFKPDNLLIRY--AGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQL 412
           LE +H +GIIHGD KPDN +IR    GE L     +     W+++G+ L+D+GR  D+ L
Sbjct: 823 LEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMRNGEDGWENKGIYLIDFGRSFDMTL 882

Query: 413 FPENVEFKGDCRTSGFRCPEMLENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGD 472
            P   +FK + +     C EM   +PW Y+ D YG   V+H+ML G ++E  +  +    
Sbjct: 883 LPPGTKFKSNWKADQQDCWEMRAGKPWSYEADYYGLAGVIHSMLFGKFIETIQLQNGRCK 942

Query: 473 FVYLPKSSFKRYWNIELWKNLFTKLLN 487
                K+ FKRYW  E+W  +F  LLN
Sbjct: 943 L----KNPFKRYWKKEIWGVIFDLLLN 963

[Close]
BLAST of PI0014103 vs. ExPASy Swiss-Prot
Match: O08901 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090 GN=Bub1 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.4e-47
Identity = 116/355 (32.68%), Postives = 183/355 (51.55%), Query Frame = 0 

Query: 173  NPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKT-IEIGGKKYEI 232
            NPW   ++  LL+          G      +YS     +S   + + KT  ++G     +
Sbjct: 714  NPWDDELILKLLS----------GLSKPVTSYSNTFEWQSKLPAIKTKTEYQLGSLLVYV 773

Query: 233  KGCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSF 292
                G G FAQV+++      ++  +    LK+Q P   WEFY+  QL  R+  +    F
Sbjct: 774  NHLLGEGAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEVHHMF 833

Query: 293  GCAHAMYLYSDYSILTCDYLAHGTLQDAINSYV-VLGKTMEEVLCIYYTIEMLYMLETLH 352
               ++ +L+ + SIL  +  ++GTL + IN Y     K M + L + + I MLYM+E +H
Sbjct: 834  IKFYSAHLFKNGSILVGELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQVH 893

Query: 353  SIGIIHGDFKPDNLLIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEF 412
            S  IIHGD KPDN ++ +         F E+       GL L+D G+ ID++LFP+   F
Sbjct: 894  SCEIIHGDIKPDNFILGH--------RFLEQADEDLATGLALIDLGQSIDMKLFPKGTVF 953

Query: 413  KGDCRTSGFRCPEMLENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKS 472
             G C TSGF+CPEML N+PW YQ+D +G    ++ ML GSYM+V+ +       V+ P+ 
Sbjct: 954  TGKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYMKVKNEGG-----VWKPEG 1013

Query: 473  SFKRYWNIELWKNLFTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKEL 522
             F+R  ++++W+  F  +LNI P  +N   L  LR++ +  +    Q   K+K L
Sbjct: 1014 LFRRLPHLDMWEEFFHIMLNI-PDCHNLPSLDFLRQNMKKLL--EQQYSNKIKTL 1042


HSP 2 Score: 57.0 bits (136), Expect = 7.5e-07
Identity = 40/133 (30.08%), Postives = 58/133 (43.61%), Query Frame = 0 

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLS 75
           +  DPL  W   IK   EN          L  LL   +  F     Y ND RF+      
Sbjct: 19  TGNDPLGEWESFIKWVEENFPDNK---EYLMTLLEHLMKEFLHKKNYHNDSRFINYCLKF 78

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEW 135
                D    F+ L  Q I   +S +Y+  A  LE++G L  A+ +++ G+   AEP E 
Sbjct: 79  AEYNSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKEL 138

Query: 136 LKKAKRLFVDRIS 149
           L++  RLF  R++
Sbjct: 139 LQQQYRLFQARLT 148

[Close]
BLAST of PI0014103 vs. ExPASy Swiss-Prot
Match: O43683 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.3e-46
Identity = 114/359 (31.75%), Postives = 183/359 (50.97%), Query Frame = 0 

Query: 173  NPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIK 232
            NPW   ++  LL+ ++  +  Y         +  +  L +++  +     ++G K   + 
Sbjct: 740  NPWDDKLIFKLLSGLSKPVSSY------PNTFEWQCKLPAIKPKTE---FQLGSKLVYVH 799

Query: 233  GCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFG 292
               G G FAQVY++      D+       LK+Q P  PWEFY+  QL  R+    +  F 
Sbjct: 800  HLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFM 859

Query: 293  CAHAMYLYSDYSILTCDYLAHGTLQDAINSYV-VLGKTMEEVLCIYYTIEMLYMLETLHS 352
              ++ +L+ + S+L  +  ++GTL +AIN Y     K M + L I + + MLYM+E +H 
Sbjct: 860  KFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHD 919

Query: 353  IGIIHGDFKPDNLLIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFK 412
              IIHGD KPDN ++   G    E + ++        GL L+D G+ ID++LFP+   F 
Sbjct: 920  CEIIHGDIKPDNFIL---GNGFLEQDDEDD----LSAGLALIDLGQSIDMKLFPKGTIFT 979

Query: 413  GDCRTSGFRCPEMLENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSS 472
              C TSGF+C EML N+PW YQ+D +G    V+ ML G+YM+V+ +          P+  
Sbjct: 980  AKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGE-----CKPEGL 1039

Query: 473  FKRYWNIELWKNLFTKLLNITPGHYNKE---LLQSLRKSFQDYMCSNPQLIRKLKELLV 524
            F+R  ++++W   F  +LNI   H+      L Q L+K FQ +  +    IR L+  L+
Sbjct: 1040 FRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNK---IRALRNRLI 1074

[Close]
BLAST of PI0014103 vs. ExPASy Swiss-Prot
Match: O94751 (Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bub1 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.7e-44
Identity = 120/380 (31.58%), Postives = 186/380 (48.95%), Query Frame = 0 

Query: 155 SMQVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSL- 214
           +M   +   S     + INP   S+ + L   + P +++   YH    +++   +++S  
Sbjct: 623 TMPYEEAMASLSNLPTLINPLDQSLRDLLFQVLRPSLLRDKDYHEHETSFALVEHIESFV 682

Query: 215 ------------RNSSRNKTIEIGGKKY----------EIKGCAGTGGFAQVY--KSYLD 274
                       R SS   +++  G ++           +    G G FA VY  KS ++
Sbjct: 683 SKIKPKAGGPGRRRSSNRHSLD--GPEFHLFYPPNTNLSVISKLGQGAFAPVYLVKSKIE 742

Query: 275 SNPDDV------------VALKIQTPPFPWEFYMYRQLDLRVDA-KERSSFGCAHAMYLY 334
           +   DV             ALKI+TPP  +EFY+ RQ   R+   +E +S    H ++++
Sbjct: 743 TENGDVSQGGAENNESKLFALKIETPPSCFEFYLTRQAMTRLKGLRETNSILPVHQLHMF 802

Query: 335 SDYSILTCDYLAHGTLQDAINSY---VVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHG 394
            D S L  DY   G++ D +NS          M+E+L ++++IE L ++E LH+  IIHG
Sbjct: 803 HDTSHLLMDYRPQGSILDLVNSMHNSTFSSSGMDEILVVFFSIEFLRIIEALHTHKIIHG 862

Query: 395 DFKPDNLLIRYAGESLTEDEFQERRGP-----WQDQGLCLVDWGRGIDLQLFPENVEFKG 454
           D K DN L+R   E++ + E+     P     W  +G+ L+D+GRGIDL LF E V+F  
Sbjct: 863 DLKADNALLRL--ETVADSEWSPIYSPEGLYGWSFKGIYLIDFGRGIDLSLFEEKVKFIA 922

Query: 455 DCRTSGFRCPEMLENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSF 489
           D  T    C EM E RPW YQ+D +G   +++TML G Y+E   +   +G    +     
Sbjct: 923 DWDTDLQDCIEMREGRPWTYQIDYHGLAAIIYTMLFGQYIETRIEV-INGQRRQVLTQRM 982

[Close]
BLAST of PI0014103 vs. ExPASy TrEMBL
Match: A0A5D3C9K3 (Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003120 PE=3 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.8e-309
Identity = 519/532 (97.56%), Postives = 526/532 (98.87%), Query Frame = 0 

Query: 1    MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
            MTI FADSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 816  MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 875

Query: 61   KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
            KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 876  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 935

Query: 121  VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
            VYELGLMRNAEPLEWLKKAKRLF+DRISELV+THSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 936  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 995

Query: 181  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
            NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF
Sbjct: 996  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 1055

Query: 241  AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
            AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV AKERSSFGCAHAMYLYSDYS
Sbjct: 1056 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 1115

Query: 301  ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
            IL CDYLA+GTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 1116 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 1175

Query: 361  LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
            L+RYAGE+LTED+FQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 1176 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 1235

Query: 421  LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
            LENRPWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 1236 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 1295

Query: 481  FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
            FTKLLNITPG YNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 1296 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 1347

[Close]
BLAST of PI0014103 vs. ExPASy TrEMBL
Match: A0A1S3C1A0 (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucumis melo OX=3656 GN=LOC103495479 PE=4 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.8e-309
Identity = 519/532 (97.56%), Postives = 526/532 (98.87%), Query Frame = 0 

Query: 1   MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           MTI FADSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 1   MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAKRLF+DRISELV+THSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
           NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF
Sbjct: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV AKERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           IL CDYLA+GTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360

Query: 361 LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           L+RYAGE+LTED+FQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENRPWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPG YNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

[Close]
BLAST of PI0014103 vs. ExPASy TrEMBL
Match: A0A5A7SMW7 (Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003080 PE=3 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 5.1e-309
Identity = 518/532 (97.37%), Postives = 526/532 (98.87%), Query Frame = 0 

Query: 1    MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
            MTI FADSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 843  MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 902

Query: 61   KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
            KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 903  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 962

Query: 121  VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
            VYELGLMRNAEPLEWLKKAKRLF+DRISELV+THSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 963  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 1022

Query: 181  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
            NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF
Sbjct: 1023 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 1082

Query: 241  AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
            AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV AKERSSFGCAHAMYLYSDYS
Sbjct: 1083 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 1142

Query: 301  ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
            IL CDYLA+GTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 1143 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 1202

Query: 361  LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
            L+RYAGE+LTED+FQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 1203 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 1262

Query: 421  LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
            LENRPWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 1263 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 1322

Query: 481  FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
            FTKLLNITPG YNKELLQSLRKSFQDYMCSNPQLIRKL+ELLVKQRASLCTS
Sbjct: 1323 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLQELLVKQRASLCTS 1374

[Close]
BLAST of PI0014103 vs. ExPASy TrEMBL
Match: A0A0A0K5T0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G050780 PE=4 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 3.7e-307
Identity = 516/532 (96.99%), Postives = 521/532 (97.93%), Query Frame = 0 

Query: 1   MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           M IFFADSQISTTTAS QDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNF GNV
Sbjct: 1   MAIFFADSQISTTTASPQDPLLPWLWSIKAALENSTSGNVSGSELAKLLSDCIGNFRGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI NSLLYIWLAAFLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICISNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAKRLF+DRISELV+THSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
           +DLLNKI+PKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF
Sbjct: 181 SDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV AKERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           IL CDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNL 360

Query: 361 LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           LIRYAGES+TED FQER GPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGESVTEDGFQERHGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENRPWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

[Close]
BLAST of PI0014103 vs. ExPASy TrEMBL
Match: A0A6J1EXJ7 (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita moschata OX=3662 GN=LOC111437097 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.0e-277
Identity = 461/532 (86.65%), Postives = 487/532 (91.54%), Query Frame = 0 

Query: 1   MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           M I   DSQI+TTT SAQDPLLPWLWSIKDALENSTSGNVSG  LA L+SDCI  F G V
Sbjct: 58  MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 117

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIW A FLESKGRLNDAN+
Sbjct: 118 KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 177

Query: 121 VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
           +YELGL+RNAEPLEWLKKAK LF+DR+SELV+THSM++ND  ESTKF ESY+NPWSSS+M
Sbjct: 178 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 237

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
            DLL+KIN KI+KY+GYHSS K Y KKM L SLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 238 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 297

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFY+YRQLD RV  KERSSFGCAH MYLYSDYS
Sbjct: 298 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 357

Query: 301 ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           IL CDYLAHGTLQDAIN YVVLGKTMEEVLCIYYTIEMLYMLETLH+IGIIHGDFKPDNL
Sbjct: 358 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 417

Query: 361 LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           LIRYA   LTED F+ER GPW DQGLCLVDWGRGIDLQLFPENVEF+GDCRTSGFRC EM
Sbjct: 418 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 477

Query: 421 LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWKYQVDIYGFCVVVHTMLHGSYM+VEKK SSDG FVYLPK+SFKRYWN+ELWKNL
Sbjct: 478 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 537

Query: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNI  G YNKELLQS+R+SFQDYMCSNPQLIRKLKELL KQRASLCT+
Sbjct: 538 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 589

[Close]
BLAST of PI0014103 vs. NCBI nr
Match: TYK06996.1 (protein TPX2 [Cucumis melo var. makuwa])

HSP 1 Score: 1070.8 bits (2768), Expect = 3.7e-309
Identity = 519/532 (97.56%), Postives = 526/532 (98.87%), Query Frame = 0 

Query: 1    MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
            MTI FADSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 816  MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 875

Query: 61   KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
            KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 876  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 935

Query: 121  VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
            VYELGLMRNAEPLEWLKKAKRLF+DRISELV+THSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 936  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 995

Query: 181  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
            NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF
Sbjct: 996  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 1055

Query: 241  AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
            AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV AKERSSFGCAHAMYLYSDYS
Sbjct: 1056 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 1115

Query: 301  ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
            IL CDYLA+GTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 1116 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 1175

Query: 361  LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
            L+RYAGE+LTED+FQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 1176 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 1235

Query: 421  LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
            LENRPWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 1236 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 1295

Query: 481  FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
            FTKLLNITPG YNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 1296 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 1347

[Close]
BLAST of PI0014103 vs. NCBI nr
Match: XP_008455277.1 (PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis melo])

HSP 1 Score: 1070.8 bits (2768), Expect = 3.7e-309
Identity = 519/532 (97.56%), Postives = 526/532 (98.87%), Query Frame = 0 

Query: 1   MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           MTI FADSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 1   MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAKRLF+DRISELV+THSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
           NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF
Sbjct: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV AKERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           IL CDYLA+GTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 301 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360

Query: 361 LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           L+RYAGE+LTED+FQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENRPWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPG YNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 481 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

[Close]
BLAST of PI0014103 vs. NCBI nr
Match: KAA0031543.1 (protein TPX2 [Cucumis melo var. makuwa])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.1e-308
Identity = 518/532 (97.37%), Postives = 526/532 (98.87%), Query Frame = 0 

Query: 1    MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
            MTI FADSQIS+TTASAQDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNFSGNV
Sbjct: 843  MTISFADSQISSTTASAQDPLLPWLWSIKGALENSTSGNVSGSELAKLLSDCIGNFSGNV 902

Query: 61   KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
            KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI
Sbjct: 903  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 962

Query: 121  VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
            VYELGLMRNAEPLEWLKKAKRLF+DRISELV+THSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 963  VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 1022

Query: 181  NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
            NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF
Sbjct: 1023 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 1082

Query: 241  AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
            AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV AKERSSFGCAHAMYLYSDYS
Sbjct: 1083 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 1142

Query: 301  ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
            IL CDYLA+GTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 1143 ILICDYLANGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 1202

Query: 361  LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
            L+RYAGE+LTED+FQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 1203 LVRYAGENLTEDDFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 1262

Query: 421  LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
            LENRPWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 1263 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 1322

Query: 481  FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
            FTKLLNITPG YNKELLQSLRKSFQDYMCSNPQLIRKL+ELLVKQRASLCTS
Sbjct: 1323 FTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLQELLVKQRASLCTS 1374

[Close]
BLAST of PI0014103 vs. NCBI nr
Match: XP_004136844.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis sativus] >KGN43647.1 hypothetical protein Csa_017323 [Cucumis sativus])

HSP 1 Score: 1063.1 bits (2748), Expect = 7.6e-307
Identity = 516/532 (96.99%), Postives = 521/532 (97.93%), Query Frame = 0 

Query: 1   MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           M IFFADSQISTTTAS QDPLLPWLWSIK ALENSTSGNVSGSELAKLLSDCIGNF GNV
Sbjct: 1   MAIFFADSQISTTTASPQDPLLPWLWSIKAALENSTSGNVSGSELAKLLSDCIGNFRGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI NSLLYIWLAAFLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICISNSLLYIWLAAFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAKRLF+DRISELV+THSMQVNDVSESTKFAESYINPWSSSIM
Sbjct: 121 VYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSIM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
           +DLLNKI+PKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF
Sbjct: 181 SDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV AKERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYS 300

Query: 301 ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           IL CDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNL 360

Query: 361 LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           LIRYAGES+TED FQER GPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGESVTEDGFQERHGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LENRPWKYQVDIYG CVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL
Sbjct: 421 LENRPWKYQVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS
Sbjct: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 532

[Close]
BLAST of PI0014103 vs. NCBI nr
Match: XP_038888854.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Benincasa hispida] >XP_038888855.1 mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1035.8 bits (2677), Expect = 1.3e-298
Identity = 497/532 (93.42%), Postives = 515/532 (96.80%), Query Frame = 0 

Query: 1   MTIFFADSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNV 60
           M IF+ DSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSG ELAKLLSDCI NFSG+V
Sbjct: 1   MAIFYPDSQISTTTASAQDPLLPWLWSIKDALENSTSGNVSGPELAKLLSDCIENFSGDV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANI 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIG+SLLYIWLA FLESKGRLNDANI
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGHSLLYIWLAVFLESKGRLNDANI 120

Query: 121 VYELGLMRNAEPLEWLKKAKRLFVDRISELVDTHSMQVNDVSESTKFAESYINPWSSSIM 180
           VYELGLMRNAEPLEWLKKAK LF+DRISELV+THSMQVNDVSESTKFAESYINPWSSS M
Sbjct: 121 VYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSMQVNDVSESTKFAESYINPWSSSTM 180

Query: 181 NDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGF 240
           NDLL+KI+PKIMKYDGYHSSTKAYSKKM LKSLRNSSRNK IEIGGKKYEIKGCAGTGGF
Sbjct: 181 NDLLSKISPKIMKYDGYHSSTKAYSKKMTLKSLRNSSRNKIIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYS 300
           AQVY+SYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRV+ KERSSFGCAHAMYLYSDYS
Sbjct: 241 AQVYRSYLDSNPDDVVALKIQTPPFPWEFYMYRQLDLRVNGKERSSFGCAHAMYLYSDYS 300

Query: 301 ILTCDYLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360
           IL CD+LAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL
Sbjct: 301 ILICDFLAHGTLQDAINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNL 360

Query: 361 LIRYAGESLTEDEFQERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEM 420
           LIRYAG++LTEDEFQER GPWQDQGLCLVDWGRGIDL LFPENVEFKGDCRTSGFRCPEM
Sbjct: 361 LIRYAGDNLTEDEFQERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEM 420

Query: 421 LENRPWKYQVDIYGFCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNL 480
           LEN+PWK+QVDIYGFCVVVHTMLHGSYMEVEKK SSDGDFVYLPK+SFKRYWNIELWKNL
Sbjct: 421 LENKPWKFQVDIYGFCVVVHTMLHGSYMEVEKKPSSDGDFVYLPKTSFKRYWNIELWKNL 480

Query: 481 FTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           FTKLLNITPG YNKEL+QS+RKSFQDYMCSNPQLIRKLKELL KQRASLC +
Sbjct: 481 FTKLLNITPGDYNKELIQSIRKSFQDYMCSNPQLIRKLKELLAKQRASLCNA 532

[Close]
BLAST of PI0014103 vs. TAIR 10
Match: AT2G20635.1 (ATP binding;protein kinases;protein serine/threonine kinases )

HSP 1 Score: 679.5 bits (1752), Expect = 2.2e-195
Identity = 316/518 (61.00%), Postives = 400/518 (77.22%), Query Frame = 0 

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLS 75
           +A DPL PWL  IK+++E+  +G  SG +L KLL DCI  +  + +Y+ND+RFLKIWFL 
Sbjct: 8   AAGDPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLY 67

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +  REDFE  ++E+ E EIC G+SLLY W A FLE KG    AN VY+ GL R AEP + 
Sbjct: 68  LEGREDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDR 127

Query: 136 LKKAKRLFVDRISELVDTHSMQ-VNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKY 195
           LK+A  LF+ RIS+     S++ V D +++T     ++NPW +S +N L++KI P+++KY
Sbjct: 128 LKEAHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKY 187

Query: 196 DGYHSSTKAYSKKMNLKSLRNSSRNKTIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDD 255
           DGYH S K +  K NL SL+N SRNK IEIGG+KY++KGCAG GGFAQV+K+++DSNPD+
Sbjct: 188 DGYHVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDE 247

Query: 256 VVALKIQTPPFPWEFYMYRQLDLRVDAKERSSFGCAHAMYLYSDYSILTCDYLAHGTLQD 315
           VVALK+Q PPFPWEF+MYRQLD R+   +RSSFG A  +++YSDYSIL CDYL+HGTLQD
Sbjct: 248 VVALKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQD 307

Query: 316 AINSYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGESLTEDEF 375
            INSYVV+GK+MEEVLC+YYTIEML MLETLHS+GIIHGDFKPDNLLIRY  E+LTE  F
Sbjct: 308 VINSYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGF 367

Query: 376 QERRGPWQDQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKYQVDIYG 435
            E+ G W  +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E++PWK+QVD YG
Sbjct: 368 HEKTGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYG 427

Query: 436 FCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGHYNK 495
            CV+VH MLH  YME+EKK S DG ++ +P++SFKRYW ++LWK LFTKLLN      + 
Sbjct: 428 LCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDT 487

Query: 496 ELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRASLCTS 533
           E L++LRKS ++Y+CS+P+L++KL ELL KQR SLC+S
Sbjct: 488 ETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

[Close]
BLAST of PI0014103 vs. TAIR 10
Match: AT2G33560.2 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 73.6 bits (179), Expect = 5.5e-13
Identity = 54/190 (28.42%), Postives = 88/190 (46.32%), Query Frame = 0 

Query: 19  DPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGM 78
           DPL PW+  IK   E    G    S L  +   C+  F  + +YK+D+R+LK+W      
Sbjct: 80  DPLSPWIECIKWVQEAFPPGG-ECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEH 139

Query: 79  REDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKK 138
             D E  +K L   EI   +++ YI  A  +E K ++  AN ++ LG+ R+A+P+E L  
Sbjct: 140 CADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLND 199

Query: 139 AKRLFVDRI---SELVDTHSMQVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYD 198
           A + F+ R    S   D    + N+   S  F  + ++   ++     L   NP+  K  
Sbjct: 200 AYKKFMVRTMRRSNTADEQEPKENNDLPSRSFG-TLLSRGDNNARRQALGSSNPQAKKLK 259

Query: 199 GYHSSTKAYS 206
              SS   ++
Sbjct: 260 PNQSSKTPFA 267

[Close]
BLAST of PI0014103 vs. TAIR 10
Match: AT5G05510.1 (Mad3/BUB1 homology region 1 )

HSP 1 Score: 73.6 bits (179), Expect = 5.5e-13
Identity = 39/132 (29.55%), Postives = 66/132 (50.00%), Query Frame = 0 

Query: 16  SAQDPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLS 75
           S +D LLPW+  +K  ++ S    +   +L + L  C  +F  + +YKND R++++W   
Sbjct: 23  SGKDHLLPWIRGVK-KMKESLPSQILNEKLPRFLQKCAESFESDKRYKNDSRYIRVWLQL 82

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEW 135
           +   +D     + +  + I    SL Y   A   E   R  DA  +Y LG+   AEP++ 
Sbjct: 83  MDFVDDPRALLRTMEAKSIGTKRSLFYQAYALHYEKMKRFEDAEKMYRLGVQNLAEPMDE 142

Query: 136 LKKAKRLFVDRI 148
           L+K+   FV R+
Sbjct: 143 LQKSYLQFVTRM 153

[Close]
BLAST of PI0014103 vs. TAIR 10
Match: AT2G33560.1 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 73.2 bits (178), Expect = 7.2e-13
Identity = 55/190 (28.95%), Postives = 89/190 (46.84%), Query Frame = 0 

Query: 19  DPLLPWLWSIKDALENSTSGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGM 78
           DPL PW+  IK   E    G    S L  +   C+  F  + +YK+D+R+LK+W      
Sbjct: 80  DPLSPWIECIKWVQEAFPPGG-ECSGLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEH 139

Query: 79  REDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKK 138
             D E  +K L   EI   +++ YI  A  +E K ++  AN ++ LG+ R+A+P+E L  
Sbjct: 140 CADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLND 199

Query: 139 AKRLFVDRI---SELVDTHSMQVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYD 198
           A + F+ R    S   D    + ND+  S  F  + ++   ++     L   NP+  K  
Sbjct: 200 AYKKFMVRTMRRSNTADEEPKENNDL-PSRSFG-TLLSRGDNNARRQALGSSNPQAKKLK 259

Query: 199 GYHSSTKAYS 206
              SS   ++
Sbjct: 260 PNQSSKTPFA 266

[Close]
BLAST of PI0014103 vs. TAIR 10
Match: AT2G25090.1 (CBL-interacting protein kinase 16 )

HSP 1 Score: 57.0 bits (136), Expect = 5.4e-08
Identity = 60/277 (21.66%), Postives = 113/277 (40.79%), Query Frame = 0 

Query: 213 LRNSSRNKTIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYMY 272
           +  S+R+ T+     KY I    GTG FA+VY     S  DDV    I+         M 
Sbjct: 1   MEESNRSSTVLF--DKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMM 60

Query: 273 RQLDLRVDAK---ERSSFGCAHAMYLYSDYSILTCDYLAHGTLQDAINSYVVLGKTMEEV 332
            Q++  +         +      +           +Y+  G L + I+     GK  E++
Sbjct: 61  EQIEREIAVMRLLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRD---GKLPEDL 120

Query: 333 LCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGESLTEDEFQERRGPWQDQGLCLV 392
              Y+  +++  ++  HS G+ H D KP+NLL+   G+               D GL  +
Sbjct: 121 ARKYFQ-QLISAVDFCHSRGVFHRDIKPENLLLDGEGDLKV-----------TDFGLSAL 180

Query: 393 DWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWK-YQVDIYGFCVVVHTMLHGSYM 452
               G+  +    +      C T  +  PE+L N+ +     DI+   +V++ +L G   
Sbjct: 181 MMPEGLGGRRGSSDDLLHTRCGTPAYVAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLP 240

Query: 453 EVEKKASSDGDFVYLPKSSFKRYWNIELWKNLFTKLL 486
            +++   +    ++  +  F  ++++E  K L ++LL
Sbjct: 241 FIDENVMTLYTKIFKAECEFPPWFSLES-KELLSRLL 259

The following BLAST results are available for this feature:
BLAST of PI0014103 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4IVI03.1e-19461.00Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana ... [more]
P416952.6e-4732.72Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (str... [more]
O089014.4e-4732.68Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090... [more]
O436831.3e-4631.75Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 ... [more]
O947512.7e-4431.58Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (st... [more]
BLAST of PI0014103 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3C9K31.8e-30997.56Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003120 P... [more]
A0A1S3C1A01.8e-30997.56mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucumis melo OX=3656 ... [more]
A0A5A7SMW75.1e-30997.37Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003080 ... [more]
A0A0A0K5T03.7e-30796.99Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G050780 PE=4 SV=1[more]
A0A6J1EXJ73.0e-27786.65mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita moschata OX... [more]
BLAST of PI0014103 vs. NCBI nr
Match NameE-valueIdentityDescription
TYK06996.13.7e-30997.56protein TPX2 [Cucumis melo var. makuwa][more]
XP_008455277.13.7e-30997.56PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis melo... [more]
KAA0031543.11.1e-30897.37protein TPX2 [Cucumis melo var. makuwa][more]
XP_004136844.17.6e-30796.99mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis sativus] >KGN43... [more]
XP_038888854.11.3e-29893.42mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Benincasa hi... [more]
BLAST of PI0014103 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G20635.12.2e-19561.00ATP binding;protein kinases;protein serine/threonine kinases [more]
AT2G33560.25.5e-1328.42BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT5G05510.15.5e-1329.55Mad3/BUB1 homology region 1 [more]
AT2G33560.17.2e-1328.95BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT2G25090.15.4e-0821.66CBL-interacting protein kinase 16 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 229..522
e-value: 2.7E-9
score: -2.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 232..448
e-value: 4.4E-16
score: 59.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 229..529
score: 18.393293
IPR013212Mad3/Bub1 homology region 1SMARTSM00777mad3_bub1_icoord: 8..126
e-value: 1.2E-10
score: 49.6
IPR013212Mad3/Bub1 homology region 1PFAMPF08311Mad3_BUB1_Icoord: 9..126
e-value: 7.3E-22
score: 77.7
IPR013212Mad3/Bub1 homology region 1PROSITEPS51489BUB1_Ncoord: 6..167
score: 32.629723
NoneNo IPR availableGENE3D1.25.40.430coord: 6..164
e-value: 1.0E-34
score: 121.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 226..529
e-value: 6.7E-99
score: 332.2
NoneNo IPR availablePANTHERPTHR14030:SF4BUB1 KINASE, ISOFORM A-RELATEDcoord: 161..531
coord: 15..148
IPR015661Mitotic spindle checkpoint protein Bub1/Mad3PANTHERPTHR14030MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 161..531
IPR015661Mitotic spindle checkpoint protein Bub1/Mad3PANTHERPTHR14030MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 15..148
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 350..362
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 226..488

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0014103.1PI0014103.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032147 activation of protein kinase activity
biological_process GO:0051754 meiotic sister chromatid cohesion, centromeric
biological_process GO:0007094 mitotic spindle assembly checkpoint signaling
biological_process GO:0060236 regulation of mitotic spindle organization
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000776 kinetochore
cellular_component GO:0005874 microtubule
cellular_component GO:0005634 nucleus
cellular_component GO:0005819 spindle
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity