PI0013678 (gene) Melon (PI 482460) v1

BLAST of PI0013678 vs. ExPASy Swiss-Prot
Match: Q9SXA1 (Phosphatidylinositol 4-kinase alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KA1 PE=1 SV=2)

HSP 1 Score: 2899.0 bits (7514), Expect = 0.0e+00
Identity = 1486/2053 (72.38%), Postives = 1702/2053 (82.90%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            M+AL ELCD+IA+NP  FSEKL+WIC RCP +E +LA SP +SRS +NAVLAVAR++SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            P+S+  R KS V EFL +IP SF  SFWP S+ +  I+SF+ +FL+Y S A +LS +F T
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EVSGFSSEVVFLSCGS-------------------VESFLPIIPSDADKLVSCILDRFFI 180
            EV+ F+ EVV  +                       + F  I+ SD DKL++ +LD+F +
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  SEAAPGTPREHNQANS-----EPSSSQSSPLSVSHQPSNGG--LSPGNENGQVSGSLSSG 240
            +  AP +P+E  Q NS     + SSSQ SP+S +  PS      SPG+E      +LSS 
Sbjct: 181  NR-APASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDEVASHGSNLSS- 240

Query: 241  ASRITDDATSASSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGAGD-------------- 300
                        S  S +MNG SI+WKSG++QL  + GF+EG  G               
Sbjct: 241  -----------KSSSSVVMNGGSIVWKSGVDQL--SFGFSEGSGGANPVFRQQVASFEDE 300

Query: 301  ----------CLQLMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSV 360
                        +L+THILD    D++L +Q+R +AK+QLQ++  FLK +KRDW+EQG V
Sbjct: 301  SIESLEKQEIAFRLITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQV 360

Query: 361  LKSRINTKLLVYQAAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLR 420
            LK+R+N KL VYQAAA++K+K++ SL+ DGK +K+L+ ET ALL+DAA+ACL SVWRK++
Sbjct: 361  LKTRVNAKLSVYQAAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMK 420

Query: 421  ICEELFGSLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTT 480
             CEELF SLL+G+A+IAVARGGQPLRVLLIRLKPLVL VC   D     QGAM ES+  T
Sbjct: 421  ACEELFDSLLSGIAKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKT 480

Query: 481  CCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVA 540
             C IIES W KDRAPVD FIMGLA+SIR+RND EEQ D+EKQ   +QLNVIRLLA + VA
Sbjct: 481  SCVIIESAWAKDRAPVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVA 540

Query: 541  VNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSY 600
            V K E+ DMILPLFIESLEEGDASTP  LRLQLLDAVSR+ATLGF+KSYRET+VLMTRSY
Sbjct: 541  VKKPEVADMILPLFIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSY 600

Query: 601  LSKLSSIGSSESRTVAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGL 660
            LSKLSS+GS ES+T APEATTERVE LPAGFL IA+GL  TKLR +YRHRLLSLCSDVGL
Sbjct: 601  LSKLSSVGSVESKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGL 660

Query: 661  AAESKSGRSGADFLGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQK 720
            AAESKSG SG DFLGPLLPAVAEICSDFDPTM++EPSLLKLFRNLWFYIALFGLAPPI K
Sbjct: 661  AAESKSGGSGVDFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVK 720

Query: 721  SQL-QTKSVSTMLNSVGS-TAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLE 780
            +     KS S  +NSVGS +A ALQAV GPY+W+ QW+ AVQ IA+GTPPLVVSSVKWLE
Sbjct: 721  TPTPPLKSTSNSVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLE 780

Query: 781  DELELNALHNPGSRRGSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFL 840
            DELELNALHNPGSRRG+GNEK A  QR ALS ALGGRVDVAAM+TISGVKATYLLAV+FL
Sbjct: 781  DELELNALHNPGSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFL 840

Query: 841  EIIRFSSNGGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWL 900
            EIIRF SNGGILNG S+V+ASRSAF CVFEYLKTPNL PAVSQCLTAIVHRAFETAVSWL
Sbjct: 841  EIIRFISNGGILNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWL 900

Query: 901  EDRISDTGNEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSC 960
            EDRIS TG +A  R+ T +AH C+LIKSMSQRDEHVRDI+VNLLTQLRDKFPQV+W+SSC
Sbjct: 901  EDRISLTGKDARNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSC 960

Query: 961  LDSLLFSMHNDAPSTVVSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKA 1020
            LDSLLFS+H++ PSTVV+DPAW   VRSLYQ+VVREWI+ SLSYAPCT QGLLQ+KLCKA
Sbjct: 961  LDSLLFSVHDNTPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKA 1020

Query: 1021 NTWQRAQHTPDVISLLSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNL 1080
            NTWQRAQ T DV+SLLSEI+IGT KNE W+GI+TANIPAV+ AAAAASGA+LK++EAFNL
Sbjct: 1021 NTWQRAQTTTDVVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNL 1080

Query: 1081 EVLSTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQ 1140
            EVL TG+VSATVKCNHAGEIAGMRRLYNSIGGFQ   +GST  GFG GLQRLI+GA  Q 
Sbjct: 1081 EVLGTGVVSATVKCNHAGEIAGMRRLYNSIGGFQ---SGSTPSGFGGGLQRLISGAFSQA 1140

Query: 1141 PQNEDDSFNGILIMKFVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTN 1200
            PQ EDDSFN +LI +FV+ LQQFV+ AEKG  ++K +FRETCSQATALLLSNL  ESKTN
Sbjct: 1141 PQPEDDSFNEMLIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTN 1200

Query: 1201 IEGFAQLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTK 1260
            +EGF+QL+RLLCWCPAYISTPDA+ETG+FIWTWLVSAAP+L S VLAELVDAW+WTIDTK
Sbjct: 1201 VEGFSQLLRLLCWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTK 1260

Query: 1261 RGLFASDA-----------SLISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSV 1320
            RGLFASD             L  GEPE  PE DPV+QI+AHR+WLGF IDRFEVVRHNS 
Sbjct: 1261 RGLFASDVRYSGPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSA 1320

Query: 1321 EQLLLFGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLE 1380
            EQLLL GR+LQ ST   W F+RHPAA G+FF+LMLLGLKFCSCQ QGN+Q F++GL+LLE
Sbjct: 1321 EQLLLLGRMLQRSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLE 1380

Query: 1381 DRIYRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENG 1440
            DRIYR SLGWFAH+PEWYDV   NF  SEA SVS+F+H+LS+E   S QSD+K + RE+G
Sbjct: 1381 DRIYRTSLGWFAHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESG 1440

Query: 1441 ISLIDSNYQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPK 1500
             +LID   QYHPVWG ++NY +G+EKR+QLLLMLCQHEADRL+VWAQP + K+S  SR K
Sbjct: 1441 -NLIDVTDQYHPVWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLK 1500

Query: 1501 LTAEKWIEHARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFV 1560
            +++EKW E+A+TAFSVDPRIA S+ SRFP NA ++ E+ QLVQ +I+D+R+IPEALPYFV
Sbjct: 1501 ISSEKWTEYAKTAFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFV 1560

Query: 1561 TPKAVDENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFM 1620
            TPK V+ENS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE+VTFFM
Sbjct: 1561 TPKNVEENSVLLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFM 1620

Query: 1621 PQLVQALRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLA 1680
            PQLVQ+LRYD+GRLVEGYLLRA QRSDIFAHILIWHLQGE  + ++ KD +  KN +F  
Sbjct: 1621 PQLVQSLRYDDGRLVEGYLLRATQRSDIFAHILIWHLQGE-DVQETPKDGSIDKNAAFQE 1680

Query: 1681 LLPVVRQHIIDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEME 1740
            +LP VRQHIIDGF+P ALD+F REFDFFDKVTSISGVLFPLPK+ERRAGIRRELEKIEM+
Sbjct: 1681 ILPQVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIEMQ 1740

Query: 1741 GEDLYLPTATNKLVRGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGD 1800
            G+DLYLPTA NKLVRGI+VDSGIPLQSAAKVPIM+TFNV+DRDGD +++KPQACIFKVGD
Sbjct: 1741 GDDLYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKVGD 1800

Query: 1801 DCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTD 1860
            DCRQDVLALQVISLLRDIFQA GLNLYLFPYGVLPTG  RGIIEVVPNTRSRSQMGETTD
Sbjct: 1801 DCRQDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGETTD 1860

Query: 1861 GGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVH 1920
            GGLYEIFQQDYGPVGS  FE ARENF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVH
Sbjct: 1861 GGLYEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVH 1920

Query: 1921 IDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRH 1980
            IDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR 
Sbjct: 1921 IDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQ 1980

Query: 1981 MDGIINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAG 1990
            MDGII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEM++REAA+FMI VCTDAYNKWTTAG
Sbjct: 1981 MDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAG 2028

BLAST of PI0013678 vs. ExPASy Swiss-Prot
Match: Q9C680 (Phosphatidylinositol 4-kinase alpha 2 OS=Arabidopsis thaliana OX=3702 GN=PI4KA2 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 4.7e-203
Identity = 351/420 (83.57%), Postives = 378/420 (90.00%), Query Frame = 0

Query: 1570 LVEGYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGF 1629
            +VEGYLLRAAQRSDIFAH LIWHLQGE S+ +  KD    KN SF  +L  VRQHI+DGF
Sbjct: 115  MVEGYLLRAAQRSDIFAHTLIWHLQGE-SVEEIVKDGAFDKNASFQEILSDVRQHIVDGF 174

Query: 1630 TPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKL 1689
            TPKAL+ F REFDFF+KVTSISG L PLPK+ER AGIRRELEKI+M+GEDLYLPTA NKL
Sbjct: 175  TPKALNFFSREFDFFEKVTSISGALLPLPKEERVAGIRRELEKIKMQGEDLYLPTAPNKL 234

Query: 1690 VRGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIS 1749
            VR IQVDSGIPLQSAAKVPIM+TFNVVD DGD N++KPQACIFKVGDDCRQDVLALQVIS
Sbjct: 235  VRVIQVDSGIPLQSAAKVPIMITFNVVDLDGDHNDVKPQACIFKVGDDCRQDVLALQVIS 294

Query: 1750 LLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGP 1809
            LL DIFQAVGLNLYLFPYGVLPT        VVPNTRSRSQMGETTDGGLYEIFQQ+YG 
Sbjct: 295  LLGDIFQAVGLNLYLFPYGVLPT--------VVPNTRSRSQMGETTDGGLYEIFQQNYGL 354

Query: 1810 VGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGG 1869
            VGS  FE AR NF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVHIDFGFILETSPGG
Sbjct: 355  VGSTTFETARANFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGG 414

Query: 1870 NMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLD 1929
            NMRFE+AHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR+MD II+TV +ML+
Sbjct: 415  NMRFENAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRYMDEIISTVQMMLE 474

Query: 1930 SGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1989
            SGLPCFSRGDPIGNLRKRFHPEM++REAA FMI VCTDAYNKWTTAGYDLIQYLQQG+EK
Sbjct: 475  SGLPCFSRGDPIGNLRKRFHPEMSEREAALFMINVCTDAYNKWTTAGYDLIQYLQQGVEK 525

HSP 2 Score: 110.9 bits (276), Expect = 1.6e-22
Identity = 54/117 (46.15%), Postives = 76/117 (64.96%), Query Frame = 0

Query: 1   MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
           M+ L ELCD+I QNP  F +KL WIC +CP S+ + A SP  S+S +NA+LAV R+LSK 
Sbjct: 1   MERLNELCDIIEQNPKQFLDKLPWICQQCPQSKLLRAESPRFSQSHLNAILAVTRILSKI 60

Query: 61  PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTD 118
            D+     K  VL+FL+++P SF  SFWP S   ++I++F+  FL Y S   +L  +
Sbjct: 61  VDTTDENAKFVVLDFLQTVPKSFHRSFWPYSLSLESISAFYCSFLGYVSCLSQLMVE 117

BLAST of PI0013678 vs. ExPASy Swiss-Prot
Match: O02811 (Phosphatidylinositol 4-kinase alpha OS=Bos taurus OX=9913 GN=PI4KA PE=2 SV=2)

HSP 1 Score: 589.0 bits (1517), Expect = 2.1e-166
Identity = 470/1693 (27.76%), Postives = 754/1693 (44.54%), Query Frame = 0

Query: 419  NQGAMFESV----LTTCCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGV 478
            +Q +M+E +    +   C  +++  T D   V+ F+     S+ +R    ++ DK+   +
Sbjct: 557  SQPSMYEQLRDIAIDNVCRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLI 616

Query: 479  PMQLNVIRLLAKMTVAVNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLG 538
            P   + IR L  + VA+  +     ++   ++ L++     P  L + ++D +  +   G
Sbjct: 617  PD--HTIRALGHIAVALRDT---PKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITG 676

Query: 539  FEKSYRETIVLMTRSYLSKLSSIGSSESRTVAPEATTERVEILPAGFLHIANGLKSTKLR 598
             +  Y+E   L  +  + K SS+  S ++             +     +IA  ++   L 
Sbjct: 677  NQYIYQEVWNLFQQISV-KASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLV 736

Query: 599  LEYRHRLLSLCSDVGLAAESKSGR-----------SGADFLGPLLPAVAEICSDFDPTMN 658
             E    LL L   +GL  +  S R           S A  LG L+P +A +     P   
Sbjct: 737  DELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKE 796

Query: 659  IEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLNSVGSTAIALQAVSGPYLWNT 718
             +P L KLFR+ W Y  L G                              AV G  LW  
Sbjct: 797  PKPRLQKLFRDFWLYSVLMGF-----------------------------AVEGSGLWPE 856

Query: 719  QWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRRGSGNEKAALAQRAALSAALG 778
            +W   V  IA  +P L   S + L   L+ N+           N+    A+ + L + + 
Sbjct: 857  EWYEGVCEIATKSPLLTFPSKEPLRSVLQYNS--------AMKNDTVTPAELSELRSTIV 916

Query: 779  GRVDVA-AMSTISG----VKATYLLAVSFLEIIRFSSNGGILNGGSNVNASRSAFCCVFE 838
              +D A  +S + G      +TYLL+V  LE +R      +L   S+  A      C FE
Sbjct: 917  NLLDPAPEVSALIGKLDFAMSTYLLSVYRLEYMR------VLR--SSDPARFQVMFCYFE 976

Query: 839  YLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRDSTLFAHTCYLIKSMS 898
                      + QC+ A+  + F+  ++ + ++     NE E     L  H  +L+ S +
Sbjct: 977  DKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEE-----LERHAQFLLVSFN 1036

Query: 899  QRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL-----------FSMHNDAPSTVVSD 958
               + +R +A   L+ L DKFP ++W+ + L ++L             +H D P   + D
Sbjct: 1037 HVHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPD 1096

Query: 959  PAWIVTVRSLYQRVVREWIVK------------SLSYAPCTCQGLLQEKLCKANTWQR-- 1018
              + +TV   Y+   RE IVK            ++ +AP   +  LQE L K   W    
Sbjct: 1097 VPYRITVPDTYE--ARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLSKHQNWVSGL 1156

Query: 1019 AQHTPDVISLLSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLST 1078
            +QHT         + + T    H+ G            + +     L    A   +  S 
Sbjct: 1157 SQHT--------GLAMATESVLHYAGYN--------KQSTSLGATQLTERPACVKKDYSN 1216

Query: 1079 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNED 1138
             M S  ++  +AGE+ GM R              S   G    L +++            
Sbjct: 1217 FMASLNLRNRYAGEVYGMIRF-------------SDATGHTSDLNKMM------------ 1276

Query: 1139 DSFNGILIMKFVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1198
                       VQ L+     A  G G D  ++ +   + TA+L+S+   +         
Sbjct: 1277 -----------VQELK-----AALGAG-DAQQYTQAMFKLTAMLISSRDCD--------P 1336

Query: 1199 QLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA 1258
            QL+  LCW P  +     +ET +  W WL++    +    + E+  AW  T++ K GLF+
Sbjct: 1337 QLLHHLCWGPLQMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWQMTVEQKFGLFS 1396

Query: 1259 SD--------ASLISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGR 1318
            ++        AS  S      PE+ P      H IW+ F + RFE+ ++ S +Q+ +F  
Sbjct: 1397 AEMKEADPLAASEASQPKPCAPEVTP------HYIWIDFLVQRFEIAKYCSSDQVEIFCS 1456

Query: 1319 LLQGSTK-----PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRI 1378
            LLQ S          + +RH AA G  F L+ LGL         N     T   +L ++I
Sbjct: 1457 LLQRSLSLSIGGTAGSMNRHVAAIGPRFKLLTLGLSLLHADVLPN----ATIRNVLREKI 1516

Query: 1379 YRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSER---GNSLQSDAKMRGRENG 1438
            Y  +  +F+  P +         +  +  +  +    S ++    + L        R N 
Sbjct: 1517 YSTAFDYFSCPPRFPTQGEKRLREDISVMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNL 1576

Query: 1439 ISLIDSNYQYHPVWGHLENYAVGRE-----------------------KRRQLLLMLCQH 1498
               + S  Q    W +    + G                         KRR LLL L   
Sbjct: 1577 DIAVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLAT 1636

Query: 1499 EADRLEVWAQP--------------NIKESTPSRPKLTAEKWIEHARTAFSVDPRIAFSM 1558
            E +RL  W  P              ++         L+ ++W ++   A+S+ P +A  +
Sbjct: 1637 EIERLITWYNPLSAPELELDQAGESSVANWRSKYISLSEKQWKDNVNLAWSISPHLAVQL 1696

Query: 1559 VSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWAACSIT 1618
             +RF     +  E+ +LV+L    +  +PEA+ + VT   +D ++  L  +  WA     
Sbjct: 1697 PARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPADPP 1756

Query: 1619 QALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYLLRAAQ 1678
              L + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD+   V  Y+L AA 
Sbjct: 1757 TGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAS 1816

Query: 1679 RSDIFAHILIWHLQGETSLPDSG--KDVNSGKNGSFLALLPVVRQHIIDGFTPKALDLFK 1738
            +S + AH  IW+++    + + G  KD + G       LL  + + I    +  A D ++
Sbjct: 1817 QSQLLAHQFIWNMKTNIYVDEEGHQKDPDIGD------LLEQLVEEITGSLSGPAKDFYQ 1876

Query: 1739 REFDFFDKVTSISGVLFPLPK-DERRAGIRRELEKIEMEGEDLYLPTATNKLVRGIQVDS 1798
            REFDFF+K+T++S ++ P PK DER+      L +++++    YLP+    +V  I   S
Sbjct: 1877 REFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PGCYLPSNPEAIVLDIDYKS 1936

Query: 1799 GIPLQSAAKVPIMVTFNV-------VDRDG----------------DPNNIKPQACIFKV 1858
            G P+QSAAK P +  F V       ++++G                D   I  QA IFKV
Sbjct: 1937 GTPMQSAAKAPYLAKFKVKRCGVSELEKEGLRCRSDPEEEGSMQEADGQKISWQAAIFKV 1996

Query: 1859 GDDCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGET 1918
            GDDCRQD+LALQ+I L ++IFQ VGL+L++FPY V+ T PG G+IE +P+  SR Q+G  
Sbjct: 1997 GDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQ 2056

Query: 1919 TDGGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRL 1978
            TD G+Y+ F + YG   +  F+ AR NFI S A Y++   LLQ KDRHNGN++ D  G L
Sbjct: 2057 TDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHL 2100

Query: 1979 VHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAAR 1988
            +HIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+GYLA R
Sbjct: 2117 IHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVRGYLAVR 2100

BLAST of PI0013678 vs. ExPASy Swiss-Prot
Match: O08662 (Phosphatidylinositol 4-kinase alpha OS=Rattus norvegicus OX=10116 GN=Pi4ka PE=1 SV=2)

HSP 1 Score: 589.0 bits (1517), Expect = 2.1e-166
Identity = 468/1687 (27.74%), Postives = 757/1687 (44.87%), Query Frame = 0

Query: 419  NQGAMFESV----LTTCCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGV 478
            NQ +M+E +    +   C  +++  T D   V+ F+     S+ +R    ++ DK+   +
Sbjct: 551  NQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLI 610

Query: 479  PMQLNVIRLLAKMTVAVNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLG 538
            P   + IR L  + VA+  +     ++   ++ L++     P  L + ++D +  +   G
Sbjct: 611  PD--HTIRALGHIAVALRDT---PKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITG 670

Query: 539  FEKSYRETIVLMTRSYLSKLSSIGSSESRTVAPEATTERVEILPAGFLHIANGLKSTKLR 598
             +  Y+E   L  +  + K SS+  S ++             +     +IA  ++   L 
Sbjct: 671  NQYIYQEVWNLFQQISV-KASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLV 730

Query: 599  LEYRHRLLSLCSDVGLAAESKSGR-----------SGADFLGPLLPAVAEICSDFDPTMN 658
             E    LL L   +GL  +  S R           S A  LG L+P +A +     P   
Sbjct: 731  DELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKE 790

Query: 659  IEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLNSVGSTAIALQAVSGPYLWNT 718
             +P L KLFR+ W Y  L G                              AV G  LW  
Sbjct: 791  AKPRLQKLFRDFWLYSVLMGF-----------------------------AVEGSGLWPE 850

Query: 719  QWSSAVQFIARGTPPLVVSSVKWLEDELELN-ALHNPGSRRGSGNEKAALAQRAALSAAL 778
            +W   V  IA  +P L   S + L   L+ N A+ N        NE      R+ +   L
Sbjct: 851  EWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELNE-----LRSTIINLL 910

Query: 779  GGRVDVAAM-STISGVKATYLLAVSFLEIIRFSSNGGILNGGSNVNASRSAFCCVFEYLK 838
                +V+A+ + +    +TYLL+V  LE +R   +       ++ +  +  F C FE   
Sbjct: 911  DPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRS-------TDPDRFQVMF-CYFEDKA 970

Query: 839  TPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRDSTLFAHTCYLIKSMSQRD 898
                   + QC+ A+  + F+  ++ + ++     NE E     L  H  +L+ + +   
Sbjct: 971  IQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEE-----LERHAQFLLVNFNHIH 1030

Query: 899  EHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL-----------FSMHNDAPSTVVSDPAW 958
            + +R +A   L+ L DKFP ++W+ + L ++L             +H D P   + D  +
Sbjct: 1031 KRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPY 1090

Query: 959  IVTVRSLYQRVVREWIVK------------SLSYAPCTCQGLLQEKLCKANTWQR--AQH 1018
             +TV   Y+   RE IVK            ++ +AP   +  LQE L K   W    +QH
Sbjct: 1091 RITVPDTYE--ARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQH 1150

Query: 1019 TPDVISLLSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTE--AFNLEVLSTG 1078
            T         + + T    H+ G    N    +T          +LTE  A   +  S  
Sbjct: 1151 T--------GLAMATESILHFAGYNKQNTTLGVT----------QLTERPACVKKDYSNF 1210

Query: 1079 MVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDD 1138
            M S  ++  +AGE+ GM R         +G  G         +Q LIT      PQ+   
Sbjct: 1211 MASLNLRNRYAGEVHGMIRF--------SGATGQMSDLNKMMVQDLITALDHSHPQH--- 1270

Query: 1139 SFNGILIMKFVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQ 1198
                                           + +   + TA+L+S+   +         Q
Sbjct: 1271 -------------------------------YTQAMFKLTAMLISSKDCD--------PQ 1330

Query: 1199 LIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFAS 1258
            L+  LCW P  +     +ET +  W WL++    +    + E+  AW  T++ K GLF++
Sbjct: 1331 LLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGLFSA 1390

Query: 1259 ---DASLISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGST 1318
               +A  ++     QP   P E +  H IW+ F + RFE+ ++ S +Q+ +F  LLQ S 
Sbjct: 1391 ETKEADPLAASEASQPRPCPPE-VTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSM 1450

Query: 1319 K-----PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLG 1378
                     + +RH AA G  F L+ LGL         N     T   +L ++IY  +  
Sbjct: 1451 SLHIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPN----ATIRNVLREKIYSTAFD 1510

Query: 1379 WFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSER---GNSLQSDAKMRGRENGISLIDS 1438
            +F+  P++         +  +  +  +    S ++    + L        R N    + S
Sbjct: 1511 YFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGS 1570

Query: 1439 NYQYHPVWGHLENYAVGRE-----------------------KRRQLLLMLCQHEADRLE 1498
              Q    W +    + G                         KRR LLL L   E +RL 
Sbjct: 1571 RQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLI 1630

Query: 1499 VWAQP--------------NIKESTPSRPKLTAEKWIEHARTAFSVDPRIAFSMVSRFPT 1558
             W  P              ++         L+ ++W ++   A+++ P +A  + +RF  
Sbjct: 1631 TWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWTISPYLAVQLPARFKN 1690

Query: 1559 NAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWAACSITQALEFL 1618
               +  E+ +LV+L    +  +PEA+ + VT   +D ++  L  +  WA       L + 
Sbjct: 1691 TEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSYF 1750

Query: 1619 TPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYLLRAAQRSDIFA 1678
            +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD+   V  Y+L AA +S + A
Sbjct: 1751 SSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAAAKSQLLA 1810

Query: 1679 HILIWHLQGETSLPDSG--KDVNSGKNGSFLALLPVVRQHIIDGFTPKALDLFKREFDFF 1738
            H  IW+++    L + G  KD + G       LL  + + I    +  A D ++REFDFF
Sbjct: 1811 HQFIWNMKTNIYLDEEGHQKDPDIGD------LLEQLVEEITGSLSGPAKDFYQREFDFF 1870

Query: 1739 DKVTSISGVLFPLPK-DERRAGIRRELEKIEMEGEDLYLPTATNKLVRGIQVDSGIPLQS 1798
            +K+T++S ++ P PK DER+      L +++++    YLP+    +V  I   SG P+QS
Sbjct: 1871 NKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PGCYLPSNPEAIVLDIDYKSGTPMQS 1930

Query: 1799 AAKVPIMVTFNV-------VDRDG----------------DPNNIKPQACIFKVGDDCRQ 1858
            AAK P +  F V       ++++G                D   I  QA IFKVGDDCRQ
Sbjct: 1931 AAKAPYLAKFKVKRCGVSELEKEGLQCRSDTEDECRRQEADGKKICWQAAIFKVGDDCRQ 1990

Query: 1859 DVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLY 1918
            D+LALQ+I L ++IFQ VGL+L++FPY V+ T PG G+IE +P+  SR Q+G  TD G+Y
Sbjct: 1991 DMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMY 2050

Query: 1919 EIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFG 1978
            + F + YG   +  F+ AR NFI S A Y++   LLQ KDRHNGN++ D  G ++HIDFG
Sbjct: 2051 DYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFG 2094

Query: 1979 FILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGI 1988
            F+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+GYLA R +MD +
Sbjct: 2111 FMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVRGYLAVRPYMDAV 2094

BLAST of PI0013678 vs. ExPASy Swiss-Prot
Match: P42356 (Phosphatidylinositol 4-kinase alpha OS=Homo sapiens OX=9606 GN=PI4KA PE=1 SV=4)

HSP 1 Score: 588.2 bits (1515), Expect = 3.5e-166
Identity = 465/1688 (27.55%), Postives = 753/1688 (44.61%), Query Frame = 0

Query: 419  NQGAMFESV----LTTCCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGV 478
            +Q +M+E +    +   C  +++  T D   V+ F+     S+ +R    ++ DK+   +
Sbjct: 557  SQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLI 616

Query: 479  PMQLNVIRLLAKMTVAVNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLG 538
            P   + IR L  + VA+  +     ++   ++ L++     P  L + ++D +  +   G
Sbjct: 617  PD--HTIRALGHIAVALRDT---PKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITG 676

Query: 539  FEKSYRETIVLMTRSYLSKLSSIGSSESRTVAPEATTERVEILPAGFLHIANGLKSTKLR 598
             +  Y+E   L  +  + K SS+  S ++             +     +IA  ++   L 
Sbjct: 677  NQYIYQEVWNLFQQISV-KASSVVYSATKDYKDHGYRHCSLAVINALANIAANIQDEHLV 736

Query: 599  LEYRHRLLSLCSDVGLAAESKSGR-----------SGADFLGPLLPAVAEICSDFDPTMN 658
             E    LL L   +GL  +  S R           S A  LG L+P +A +     P   
Sbjct: 737  DELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPPIKE 796

Query: 659  IEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLNSVGSTAIALQAVSGPYLWNT 718
             +P L KLFR+ W Y  L G                              AV G  LW  
Sbjct: 797  AKPRLQKLFRDFWLYSVLMGF-----------------------------AVEGSGLWPE 856

Query: 719  QWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRRGSGNEKAALAQRAALSAALG 778
            +W   V  IA  +P L   S + L   L+ N+           N+    A+ + L + + 
Sbjct: 857  EWYEGVCEIATKSPLLTFPSKEPLRSVLQYNS--------AMKNDTVTPAELSELRSTII 916

Query: 779  GRVD-----VAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGSNVNASRSAFCCVFE 838
              +D      A ++ +    +TYLL+V  LE +R   +       ++ +  +  F C FE
Sbjct: 917  NLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRS-------TDPDRFQVMF-CYFE 976

Query: 839  YLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRDSTLFAHTCYLIKSMS 898
                      + QC+ A+  + F+  ++ + D+     NE E     L  H  +L+ + +
Sbjct: 977  DKAIQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTKENEEE-----LERHAQFLLVNFN 1036

Query: 899  QRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL-----------FSMHNDAPSTVVSD 958
               + +R +A   L+ L DKFP ++W+ + L ++L             +H D P   + D
Sbjct: 1037 HIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPD 1096

Query: 959  PAWIVTVRSLYQRVVREWIVK------------SLSYAPCTCQGLLQEKLCKANTWQR-- 1018
              + +TV   Y+   RE IVK            ++ +AP   +  LQE L K   W    
Sbjct: 1097 APYRITVPDTYE--ARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGL 1156

Query: 1019 AQHTPDVISLLSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLST 1078
            +QHT         + + T    H+ G    N               L    A   +  S 
Sbjct: 1157 SQHT--------GLAMATESILHFAGYNKQN--------TTLGATQLSERPACVKKDYSN 1216

Query: 1079 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNED 1138
             M S  ++  +AGE+ GM R            +G+TG                   Q  D
Sbjct: 1217 FMASLNLRNRYAGEVYGMIRF-----------SGTTG-------------------QMSD 1276

Query: 1139 DSFNGILIMKFVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1198
                  L    VQ L   +  +          + +   + TA+L+S+   +         
Sbjct: 1277 ------LNKMMVQDLHSALDRSHPQH------YTQAMFKLTAMLISSKDCD--------P 1336

Query: 1199 QLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA 1258
            QL+  LCW P  +     +ET +  W WL++    +    + E+  AW  T++ K GLF+
Sbjct: 1337 QLLHHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFMREMAGAWHMTVEQKFGLFS 1396

Query: 1259 S---DASLISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGS 1318
            +   +A  ++     QP+  P E +  H IW+ F + RFE+ ++ S +Q+ +F  LLQ S
Sbjct: 1397 AEIKEADPLAASEASQPKPCPPE-VTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRS 1456

Query: 1319 TK-----PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASL 1378
                      + +RH AA G  F L+ LGL         N     T   +L ++IY  + 
Sbjct: 1457 MSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPN----ATIRNVLREKIYSTAF 1516

Query: 1379 GWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSER---GNSLQSDAKMRGRENGISLID 1438
             +F+  P++         +  +  +  +    S ++    + L        R N    + 
Sbjct: 1517 DYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVG 1576

Query: 1439 SNYQYHPVWGHLENYAVGRE-----------------------KRRQLLLMLCQHEADRL 1498
            S  Q    W +    + G                         KRR LLL L   E +RL
Sbjct: 1577 SRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERL 1636

Query: 1499 EVWAQP--------------NIKESTPSRPKLTAEKWIEHARTAFSVDPRIAFSMVSRFP 1558
              W  P              ++         L+ ++W ++   A+S+ P +A  + +RF 
Sbjct: 1637 ITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFK 1696

Query: 1559 TNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPHWAACSITQALEF 1618
                +  E+ +LV+L    +  +PEA+ + VT   +D ++  L  +  WA       L +
Sbjct: 1697 NTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCWAPTDPPTGLSY 1756

Query: 1619 LTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEGYLLRAAQRSDIF 1678
             +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD+   V  Y+L AA +S + 
Sbjct: 1757 FSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASKSQLL 1816

Query: 1679 AHILIWHLQGETSLPDSG--KDVNSGKNGSFLALLPVVRQHIIDGFTPKALDLFKREFDF 1738
            AH  IW+++    L + G  KD + G       LL  + + I    +  A D ++REFDF
Sbjct: 1817 AHQFIWNMKTNIYLDEEGHQKDPDIGD------LLDQLVEEITGSLSGPAKDFYQREFDF 1876

Query: 1739 FDKVTSISGVLFPLPK-DERRAGIRRELEKIEMEGEDLYLPTATNKLVRGIQVDSGIPLQ 1798
            F+K+T++S ++ P PK DER+      L +++++    YLP+    +V  I   SG P+Q
Sbjct: 1877 FNKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PGCYLPSNPEAIVLDIDYKSGTPMQ 1936

Query: 1799 SAAKVPIMVTFNV-------VDRDG----------------DPNNIKPQACIFKVGDDCR 1858
            SAAK P +  F V       ++++G                D   I  QA IFKVGDDCR
Sbjct: 1937 SAAKAPYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCR 1996

Query: 1859 QDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGL 1918
            QD+LALQ+I L ++IFQ VGL+L++FPY V+ T PG G+IE +P+  SR Q+G  TD G+
Sbjct: 1997 QDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGM 2056

Query: 1919 YEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDF 1978
            Y+ F + YG   +  F+ AR NFI S A Y++   LLQ KDRHNGN++ D  G ++HIDF
Sbjct: 2057 YDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDF 2100

Query: 1979 GFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDG 1988
            GF+ E+SPGGN+ +E    KL+ EM  ++   G M++  +  F+ +CV+GYLA R +MD 
Sbjct: 2117 GFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEATPFKWFMEMCVRGYLAVRPYMDA 2100

BLAST of PI0013678 vs. ExPASy TrEMBL
Match: A0A1S3BGG8 (phosphatidylinositol 4-kinase alpha 1-like OS=Cucumis melo OX=3656 GN=LOC103489597 PE=4 SV=1)

HSP 1 Score: 3820.8 bits (9907), Expect = 0.0e+00
Identity = 1946/2037 (95.53%), Postives = 1960/2037 (96.22%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            MDALIELCDLIAQNPA FSEKLSWICSRCPPSEAILAGSP ISRSQ+NAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPTISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            PDSVGLRPKS VLEFLRSIPLSFSLSFWPQSYG+DAIASFFNEFLNYTSKACELSTDFAT
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGHDAIASFFNEFLNYTSKACELSTDFAT 120

Query: 121  EVSGFSSEVVF-----LSCGS----------VESFLPIIPSDADKLVSCILDRFFISEAA 180
            EVSGFSSEVV       S GS           +SFLPIIPSDADKLVSCILDRF ISEA 
Sbjct: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALTKSFLPIIPSDADKLVSCILDRFLISEAI 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            P TPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PETPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGA----------------------GDCLQ 300
            SSRGSGMMNGNSILWKSGLEQLGMNLGF+EGG                           +
Sbjct: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINTKLLVYQ 360
            LMTHILDNSSFDARLWEQM+ LAKKQLQTLPTFLKIKKRDWSEQGSVLK+RINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMKTLAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660
            TVAPEATTERVEILPAGFLHIANGLKS KLRLEYRHRLLSLCSDVGLAAESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLHIANGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRD 900
            SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAE RD
Sbjct: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEARD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLR+KFPQVMWNSSCLDSLLFSMHNDAPST
Sbjct: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLREKFPQVMWNSSCLDSLLFSMHNDAPST 960

Query: 961  VVSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020
            VVSDPAW+VTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL
Sbjct: 961  VVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGTSKNEHWTG QTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTSKNEHWTGTQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKG GLDKLKFRETCSQATALLLSNL SESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLDSESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDAS------ 1260
            AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASD        
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDIKYSGPAA 1260

Query: 1261 -----LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
                 L  GEPE+QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLIDSNYQYHPVWG 1440
            EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAK+RGRENGISLIDSN  YHPVWG
Sbjct: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKIRGRENGISLIDSNDHYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKLTAEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPYRPKLTAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680
            GYLLRAA+RSDIFAHILIWHLQGETS PDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK
Sbjct: 1621 GYLLRAAKRSDIFAHILIWHLQGETSSPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740
            ALDLF+REFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEM+GEDLYLPTATNKLVRG
Sbjct: 1681 ALDLFRREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMDGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1990
            PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2037

BLAST of PI0013678 vs. ExPASy TrEMBL
Match: A0A6J1GKT2 (phosphatidylinositol 4-kinase alpha 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454915 PE=4 SV=1)

HSP 1 Score: 3728.7 bits (9668), Expect = 0.0e+00
Identity = 1892/2036 (92.93%), Postives = 1936/2036 (95.09%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            MDALIELCDLIAQNPA FSEKLSWICSRCPPSEAILAGSPAISRSQ+NAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            PDSVGLRPKS VLEFLRSIPLSFSLSFWPQS+GNDAIASFF EFLNYTS+ACELSTDFA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSFGNDAIASFFTEFLNYTSQACELSTDFAM 120

Query: 121  EVSGFSSEVVF-----LSCGSV----------ESFLPIIPSDADKLVSCILDRFFISEAA 180
            EVSGF+SEVV       S GS           +SFLPIIPSDADKLVSCILD F ISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISSAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  QANSEPSSSQSSPLSVSHQP NGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPLNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGAGDCLQ---------------------L 300
            SSRGSGMMNGNSILWKSGL+QLGMNLG +EGG     Q                     L
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSEGGLAFVRQQVAFFEDESIENLEKQEIAFKL 300

Query: 301  MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINTKLLVYQA 360
            MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLK+RINTKLLVYQA
Sbjct: 301  MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQA 360

Query: 361  AARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA 420
            AARLKM TVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA
Sbjct: 361  AARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA 420

Query: 421  QIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDRA 480
            QIAV RGGQPLRVLLIRLKPL LTVC QADTWG+NQGAMFESVLTTCCEIIESCWTKDRA
Sbjct: 421  QIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDRA 480

Query: 481  PVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPLF 540
            PVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKM+ AVNKSEIV+MILPLF
Sbjct: 481  PVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPLF 540

Query: 541  IESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESRT 600
            IESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVL+TRSYLSKLSSIGSSESRT
Sbjct: 541  IESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESRT 600

Query: 601  VAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKSGRSGADFL 660
            VAPEATTERVEILPAGFLHIA+GLKS KLRLEYRHRLL LCSDVGLAAESKSGRSGADFL
Sbjct: 601  VAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADFL 660

Query: 661  GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLNS 720
            GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVS+MLNS
Sbjct: 661  GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLNS 720

Query: 721  VGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRRG 780
            VGSTAIALQAVSGPYLWNTQWSSAVQFIA+GTPPLVVSSVKWLEDELELNALHNPGSRRG
Sbjct: 721  VGSTAIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRRG 780

Query: 781  SGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS 840
            SGNEKAA+AQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS
Sbjct: 781  SGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS 840

Query: 841  NVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRDS 900
            NVNA+RSAF CVFEYLKTPNLLPAV+QCLTAIVHRAFETAVSWLEDRIS+TGNEAEVRDS
Sbjct: 841  NVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVRDS 900

Query: 901  TLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPSTV 960
            TL+AHTCYLIK MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA STV
Sbjct: 901  TLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASSTV 960

Query: 961  VSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL 1020
            VSDPAW+VTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL
Sbjct: 961  VSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL 1020

Query: 1021 SEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH 1080
            SEIRIGT KN++WTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH
Sbjct: 1021 SEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH 1080

Query: 1081 AGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMKF 1140
            AGEIAGMRRLYNSIGGFQTGVAG TGLGFGQGLQRLITG LPQQPQNEDDSFNGILIMKF
Sbjct: 1081 AGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIMKF 1140

Query: 1141 VQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA 1200
            VQSLQQFVSGAEKG GLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA
Sbjct: 1141 VQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA 1200

Query: 1201 YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDAS------- 1260
            YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFAS+         
Sbjct: 1201 YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAAK 1260

Query: 1261 ----LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP 1320
                L  GEPE QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP
Sbjct: 1261 LRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP 1320

Query: 1321 PWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEPE 1380
            PWNFSRHPAATGSFFTLMLLGLKF SCQ+QGNLQNFKTGLELLEDRIYRASLGWFAHEPE
Sbjct: 1321 PWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHEPE 1380

Query: 1381 WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLIDSNYQYHPVWGH 1440
            WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSD K RGRENGISL+DSN QYHPVWGH
Sbjct: 1381 WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVWGH 1440

Query: 1441 LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSVD 1500
            LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP+RPKL+AEKWIEHARTAFSVD
Sbjct: 1441 LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPARPKLSAEKWIEHARTAFSVD 1500

Query: 1501 PRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPH 1560
            PRIAFSMVSRFPTN FLR E+ QLVQLHILDIRSIPEALPYF+TPKAV+ENSELLRQLPH
Sbjct: 1501 PRIAFSMVSRFPTNGFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVEENSELLRQLPH 1560

Query: 1561 WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEG 1620
            WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLVEG
Sbjct: 1561 WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLVEG 1620

Query: 1621 YLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPKA 1680
            YLLRAAQRSDIFAHILIWHLQGETS+PDSGKD NSGKN SFLALLP+VR HIIDGFTPKA
Sbjct: 1621 YLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTPKA 1680

Query: 1681 LDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI 1740
             DLF+REFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI
Sbjct: 1681 RDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI 1740

Query: 1741 QVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLRD 1800
            QVDSGIPLQSAAKVPIM+TFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVI+LLRD
Sbjct: 1741 QVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALLRD 1800

Query: 1801 IFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860
            IFQAVGLNLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP
Sbjct: 1801 IFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860

Query: 1861 NFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF 1920
            +FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF
Sbjct: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF 1920

Query: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP 1980
            ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP
Sbjct: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP 1980

Query: 1981 CFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1990
            CFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of PI0013678 vs. ExPASy TrEMBL
Match: A0A6J1I6A0 (phosphatidylinositol 4-kinase alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471091 PE=4 SV=1)

HSP 1 Score: 3724.1 bits (9656), Expect = 0.0e+00
Identity = 1890/2036 (92.83%), Postives = 1935/2036 (95.04%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            MDALIELCDLIAQNPA FSEKLSWICSRCPPSEAILAGSPAISRSQ+NAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            P+SVGLRPKS VLEFLRSIPLSFSLSFWPQS+GNDAIASFF EFLNYTSKACELSTDFA 
Sbjct: 61   PESVGLRPKSVVLEFLRSIPLSFSLSFWPQSFGNDAIASFFTEFLNYTSKACELSTDFAM 120

Query: 121  EVSGFSSEVVF-----LSCGSV----------ESFLPIIPSDADKLVSCILDRFFISEAA 180
            EVSGF+SEVV       S GS           +SFLPIIPSDADKLVSCILD F ISE  
Sbjct: 121  EVSGFTSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  QANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGAGDCLQ---------------------L 300
            SSRGSGMMNGNSILWKSGL+QLGMNLG +EGG     Q                     L
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSEGGVAFVRQQVAFFEDESIENLEKQEIAFKL 300

Query: 301  MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINTKLLVYQA 360
            MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLK+RINTKLLVYQA
Sbjct: 301  MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQA 360

Query: 361  AARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA 420
            AARLKM TVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA
Sbjct: 361  AARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA 420

Query: 421  QIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDRA 480
            QIAV RGGQPLRVLLIRLKPL LTVC QADTWG+NQGAMFESVLTTCCEIIESCWTKDRA
Sbjct: 421  QIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDRA 480

Query: 481  PVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPLF 540
            PVDTFIMGLATSIRDRNDSEEQDDKEKQ VP QLNVIRLLAKM+ AVNKSEIV+MILPLF
Sbjct: 481  PVDTFIMGLATSIRDRNDSEEQDDKEKQAVPTQLNVIRLLAKMSAAVNKSEIVEMILPLF 540

Query: 541  IESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESRT 600
            IESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVL+TRSYLSKLSSIGSSESRT
Sbjct: 541  IESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESRT 600

Query: 601  VAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKSGRSGADFL 660
            VAPEATTERVEILPAGFLHIA+GLKS KLRLEYRHRLL LCSDVGLAAESKSGRSGADFL
Sbjct: 601  VAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADFL 660

Query: 661  GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLNS 720
            GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVS+MLNS
Sbjct: 661  GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLNS 720

Query: 721  VGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRRG 780
            VGSTAIALQAVSGPYLWNTQWSSAVQFIA+GTPPLVVSSVKWLEDELELNALHNPGSRRG
Sbjct: 721  VGSTAIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRRG 780

Query: 781  SGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS 840
            SGNEKAA+AQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS
Sbjct: 781  SGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS 840

Query: 841  NVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRDS 900
            NVNA+RSAF CVFEYLKTPNLLPAV+QCLTAIVHRAFETAVSWLEDRIS+TGNEAEVRDS
Sbjct: 841  NVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVRDS 900

Query: 901  TLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPSTV 960
            TL+AHTCYLIK MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA STV
Sbjct: 901  TLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASSTV 960

Query: 961  VSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL 1020
            VSDP+W+VTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL
Sbjct: 961  VSDPSWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL 1020

Query: 1021 SEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH 1080
            SEIRIGT KN++WTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH
Sbjct: 1021 SEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH 1080

Query: 1081 AGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMKF 1140
            AGEIAGMRRLYNSIGGFQTGV G TGLGFGQGLQRLITGALPQQ QNEDDSFNGILIMKF
Sbjct: 1081 AGEIAGMRRLYNSIGGFQTGVTGPTGLGFGQGLQRLITGALPQQSQNEDDSFNGILIMKF 1140

Query: 1141 VQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA 1200
            VQSLQQFVSGAEKG GLDKLKFRETCSQATALLLSNLASE+KTNIEGFAQLIRLLCWCPA
Sbjct: 1141 VQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASEAKTNIEGFAQLIRLLCWCPA 1200

Query: 1201 YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDAS------- 1260
            YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFAS+         
Sbjct: 1201 YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAAK 1260

Query: 1261 ----LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP 1320
                L  GEPE QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP
Sbjct: 1261 LRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP 1320

Query: 1321 PWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEPE 1380
            PWNFS HPAATGSFFTLMLLGLKF SCQ+QGNLQNFKTGLELLEDRIYRASLGWFAHEPE
Sbjct: 1321 PWNFSHHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHEPE 1380

Query: 1381 WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLIDSNYQYHPVWGH 1440
            WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAK RGRENGISL+DSN QYHPVWGH
Sbjct: 1381 WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKTRGRENGISLVDSNDQYHPVWGH 1440

Query: 1441 LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSVD 1500
            LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP+RPKL+AEKWIEHARTAFSVD
Sbjct: 1441 LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPARPKLSAEKWIEHARTAFSVD 1500

Query: 1501 PRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPH 1560
            PRIAFSMVSRFPTN FLR E+ QLVQLHILDIRSIPEALPYF+TPKAVDENSELLRQLPH
Sbjct: 1501 PRIAFSMVSRFPTNVFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVDENSELLRQLPH 1560

Query: 1561 WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEG 1620
            WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLVEG
Sbjct: 1561 WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLVEG 1620

Query: 1621 YLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPKA 1680
            YLLRAAQRSDIFAHILIWHLQGETS+PDSGKD NSGKN SFLALLP+VR HII+GFTPKA
Sbjct: 1621 YLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIINGFTPKA 1680

Query: 1681 LDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI 1740
             DLF+REFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI
Sbjct: 1681 RDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI 1740

Query: 1741 QVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLRD 1800
            QVDSGIPLQSAAKVPIM+TFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVI+LLRD
Sbjct: 1741 QVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALLRD 1800

Query: 1801 IFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860
            IFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP
Sbjct: 1801 IFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860

Query: 1861 NFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF 1920
            +FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF
Sbjct: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF 1920

Query: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP 1980
            ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP
Sbjct: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP 1980

Query: 1981 CFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1990
            CFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of PI0013678 vs. ExPASy TrEMBL
Match: A0A6J1C3T4 (phosphatidylinositol 4-kinase alpha 1-like OS=Momordica charantia OX=3673 GN=LOC111007670 PE=4 SV=1)

HSP 1 Score: 3619.7 bits (9385), Expect = 0.0e+00
Identity = 1840/2047 (89.89%), Postives = 1909/2047 (93.26%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            M+ALIELCDLIAQNPA FSEKLSWICSRCPPSE  LAGSPAISRSQ+NAVLAVARLLSKC
Sbjct: 1    MEALIELCDLIAQNPALFSEKLSWICSRCPPSEVFLAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            PDSVGLRPKS V+EF+RSIPLSF+LSFWPQSYGNDAIASF+ EF+NYTSKACELST+FAT
Sbjct: 61   PDSVGLRPKSVVIEFIRSIPLSFNLSFWPQSYGNDAIASFYAEFMNYTSKACELSTEFAT 120

Query: 121  EVSGFSSEVVF-----LSCGS----------VESFLPIIPSDADKLVSCILDRFFISEAA 180
            E+SGF+SEVV       S GS           +SF+PI+PSDADKLVSCILDRF ISEAA
Sbjct: 121  EISGFTSEVVLSAIDDCSEGSPISRAFLVAMSKSFIPILPSDADKLVSCILDRFLISEAA 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  +ANSE SSSQ SPLSVSH P NGGLSPGNENGQVSGSLSSG SRITDDATSA
Sbjct: 181  PGTPREQIRANSEASSSQGSPLSVSHHPLNGGLSPGNENGQVSGSLSSGGSRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGA----------------------GDCLQ 300
            SSRGSGMMNG+S+LWKSG++QLGMNLG +EGG                           +
Sbjct: 241  SSRGSGMMNGSSLLWKSGVDQLGMNLGVSEGGGVASVRQQVTVFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINTKLLVYQ 360
            L+THILDNSSFDARLWEQMRAL KKQLQTLPTFLKIKKRDWSEQGSVLK+RINTKLLVYQ
Sbjct: 301  LVTHILDNSSFDARLWEQMRALLKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKMK VASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMKAVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVC QADTWG++QGAMFESVL TCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCAQADTWGSSQGAMFESVLKTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKMTVAVNKSE+VDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEVVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660
            T+APEA TERVE LPAGFL IA+GLK  KLR EYRHRLLSLCSDVGL+AESKSGRSGADF
Sbjct: 601  TLAPEANTERVETLPAGFLLIASGLKGAKLRSEYRHRLLSLCSDVGLSAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVS+MLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLN 720

Query: 721  SVGST-AIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSR 780
            SVGS  AIALQAVSGPY+WNTQWSSAVQFIA+GTPPLVVSSVKWLEDELELNALHNPGSR
Sbjct: 721  SVGSMGAIALQAVSGPYMWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSR 780

Query: 781  RGSGNEKAALAQR----AALSAALGGRVDVAAMSTIS-----GVKATYLLAVSFLEIIRF 840
            +GSGNEKAAL+        L       V     S IS     GVKATYLLAVSFLEIIRF
Sbjct: 781  QGSGNEKAALSVAYYIVYCLDVFFSRMVFSFLPSQISLFHFVGVKATYLLAVSFLEIIRF 840

Query: 841  SSNGGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRIS 900
            SSNGG+LNGGSN+NASRSAF CVFEYLKTPNLLPAVSQCLTA+VH AFETAVSWL+DRIS
Sbjct: 841  SSNGGVLNGGSNLNASRSAFSCVFEYLKTPNLLPAVSQCLTAMVHGAFETAVSWLDDRIS 900

Query: 901  DTGNEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL 960
            +TGNEAEVRDSTLFAHTC+LI+SMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL
Sbjct: 901  ETGNEAEVRDSTLFAHTCFLIRSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLL 960

Query: 961  FSMHNDAPSTVVSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQR 1020
            FSMHNDAPS VV+DPAW+VTVRSLYQ VVREWI+KSLSYAPCT QGLLQEKLCK NTWQR
Sbjct: 961  FSMHNDAPSAVVTDPAWVVTVRSLYQGVVREWIIKSLSYAPCTSQGLLQEKLCKVNTWQR 1020

Query: 1021 AQHTPDVISLLSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLST 1080
            AQHTPDVISLLSEIRIGT KN+ WTGIQTANIPAV+TAAAAASGADLKLTEAFNLEVL T
Sbjct: 1021 AQHTPDVISLLSEIRIGTGKNDCWTGIQTANIPAVMTAAAAASGADLKLTEAFNLEVLGT 1080

Query: 1081 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNED 1140
            GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVA  TGLGFGQG QRLI+GA PQ+PQNED
Sbjct: 1081 GMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAAPTGLGFGQGFQRLISGAFPQKPQNED 1140

Query: 1141 DSFNGILIMKFVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1200
            DSFNGILIMKFVQSLQQFVSGAEKG GLDKLKFRETCSQATALLLSNLASESKTNIEGFA
Sbjct: 1141 DSFNGILIMKFVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFA 1200

Query: 1201 QLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA 1260
            +LIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA
Sbjct: 1201 RLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFA 1260

Query: 1261 SDAS-----------LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLL 1320
            S+             L  GEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQ+LL
Sbjct: 1261 SEVKYSGPAAKLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQVLL 1320

Query: 1321 FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYR 1380
            FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQ+QGNLQNFKTGLELLEDRIYR
Sbjct: 1321 FGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRIYR 1380

Query: 1381 ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLID 1440
            ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSS+RGN LQ DAK RGRENGIS+++
Sbjct: 1381 ASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSDRGNGLQFDAKTRGRENGISVVN 1440

Query: 1441 SNYQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKW 1500
            S  QYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKL++EKW
Sbjct: 1441 SKDQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSSEKW 1500

Query: 1501 IEHARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD 1560
            IEH RTAFSVDPRIAFSMVSRFPTNA LRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD
Sbjct: 1501 IEHVRTAFSVDPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVD 1560

Query: 1561 ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA 1620
            ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA
Sbjct: 1561 ENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQA 1620

Query: 1621 LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVR 1680
            LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETS+PDSGKD NSGKNGSFLALLPVVR
Sbjct: 1621 LRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNGSFLALLPVVR 1680

Query: 1681 QHIIDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL 1740
            QHIIDGFTPKALDLF+REF+FFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL
Sbjct: 1681 QHIIDGFTPKALDLFEREFNFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYL 1740

Query: 1741 PTATNKLVRGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDV 1800
            PTATNKLVRGIQVDSGIPLQSAAKVPIM+TFNVV+RDGDPN+IKPQACIFKVGDDCRQDV
Sbjct: 1741 PTATNKLVRGIQVDSGIPLQSAAKVPIMITFNVVNRDGDPNDIKPQACIFKVGDDCRQDV 1800

Query: 1801 LALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI 1860
            LALQVI+LLRDIFQAVG+NLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI
Sbjct: 1801 LALQVIALLRDIFQAVGINLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEI 1860

Query: 1861 FQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFI 1920
            FQQDYGPVGSPNFEAARENFI+SSAGYAVASLLLQPKDRHNGNLL DN GRLVHIDFGFI
Sbjct: 1861 FQQDYGPVGSPNFEAARENFIISSAGYAVASLLLQPKDRHNGNLLIDNTGRLVHIDFGFI 1920

Query: 1921 LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN 1980
            LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN
Sbjct: 1921 LETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIIN 1980

Query: 1981 TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQY 1990
            TVLLMLDSGLPCFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQY
Sbjct: 1981 TVLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQY 2040

BLAST of PI0013678 vs. ExPASy TrEMBL
Match: A0A6P3YUH8 (phosphatidylinositol 4-kinase alpha 1 OS=Ziziphus jujuba OX=326968 GN=LOC107404942 PE=4 SV=1)

HSP 1 Score: 3196.8 bits (8287), Expect = 0.0e+00
Identity = 1617/2046 (79.03%), Postives = 1800/2046 (87.98%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            M+ALIELCDLIAQNP  FSEKLSWIC RCPP E++L GSP +SRSQ+NAVLAVAR LSKC
Sbjct: 1    MEALIELCDLIAQNPVQFSEKLSWICGRCPPPESLLEGSPRVSRSQLNAVLAVARFLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
             DS  LRPKS VLEFLR IP SF+ SFWPQS+GNDAIASFF +FL Y SKA ELS DFAT
Sbjct: 61   QDSADLRPKSVVLEFLRFIPASFNQSFWPQSFGNDAIASFFADFLGYVSKAPELSPDFAT 120

Query: 121  EVSGFSSEV----------------VFLSCGSVESFLPIIPSDADKLVSCILDRFFIS-- 180
            E++GF+ +V                VFL+  S +SF PI  SDA++LV+ +LD+   S  
Sbjct: 121  EIAGFTGDVVVSAISNASADLGISRVFLTALS-QSFPPISASDAERLVNLLLDQLAASGT 180

Query: 181  --EAAPGTPREHNQANSEPSSSQSSPLSVSH-QPSNGGLSPGNENGQVSGSLSSGASRIT 240
                +P TPRE   ANSE SSSQSSPLS++H QP  G  SPGNE   VSGS  S ASRI 
Sbjct: 181  IVAQSPATPREQITANSETSSSQSSPLSLNHSQPHGGSSSPGNEASNVSGSSGSAASRIA 240

Query: 241  DDATSASSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGAGDCL----------------- 300
            DDATSASSRGS MMNG SILWKSG++QLG+N G+N+GG    L                 
Sbjct: 241  DDATSASSRGSMMMNGGSILWKSGVDQLGVNFGYNDGGGAMLLRQQVSSFEEESVESLEK 300

Query: 301  -----QLMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINT 360
                 +L+ HIL+    D  L EQ+R +AK+QLQ+L  FLKI+KRDW+E G++LK+RINT
Sbjct: 301  QEIAFKLIAHILEKCCIDMGLLEQVRFIAKRQLQSLTVFLKIRKRDWNEHGTLLKARINT 360

Query: 361  KLLVYQAAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFG 420
            KLLVY+AAA+L +KT+     DGK AKKL  ET AL MDAAE+CLLSVWRKLRICEELFG
Sbjct: 361  KLLVYKAAAKLTLKTLDCFHSDGKLAKKLAHETLALFMDAAESCLLSVWRKLRICEELFG 420

Query: 421  SLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIES 480
            SLL GL+QIAV RGGQ LR+LLIRLKP++LTVC QADTW T+QGAMFESV+ T C+IIES
Sbjct: 421  SLLAGLSQIAVHRGGQSLRILLIRLKPVILTVCTQADTWATSQGAMFESVMKTTCQIIES 480

Query: 481  CWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGVP-MQLNVIRLLAKMTVAVNKSEI 540
            CW K+RAPVDTFIMGLATSIR+RND EEQ DK+K+ +P MQLNVIRLLA + VAVNKSE+
Sbjct: 481  CWAKERAPVDTFIMGLATSIRERNDYEEQVDKDKETIPVMQLNVIRLLADLNVAVNKSEV 540

Query: 541  VDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSS 600
            VDMILPLFIESLEEGDASTP LLRL+LLDAVSRMA+LGFEKSYRET+VLMTRSYL+KLSS
Sbjct: 541  VDMILPLFIESLEEGDASTPSLLRLRLLDAVSRMASLGFEKSYRETVVLMTRSYLNKLSS 600

Query: 601  IGSSESRTVAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKS 660
            +GS+ES+TVA EATTERVE LP+GF  IA+GL +TKLR +YRHRLLSLCSDVGLAAESKS
Sbjct: 601  VGSAESKTVAAEATTERVETLPSGFHLIASGLTNTKLRADYRHRLLSLCSDVGLAAESKS 660

Query: 661  GRSGADFLGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTK 720
            GRSGADFLGPLLPAVAEICSDFDP++++EPSLLKLFRNLWFYIALFGLAPPIQK Q  TK
Sbjct: 661  GRSGADFLGPLLPAVAEICSDFDPSVDVEPSLLKLFRNLWFYIALFGLAPPIQKIQHPTK 720

Query: 721  SVSTMLNSVGST-AIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNA 780
             VST LNSVGS   IALQAV GPY+WNTQWSSAVQ IA+GTP LVVSSVKWLEDELELNA
Sbjct: 721  PVSTTLNSVGSMGTIALQAVGGPYMWNTQWSSAVQQIAQGTPSLVVSSVKWLEDELELNA 780

Query: 781  LHNPGSRRGSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSS 840
            LHNPGSRRGSGNEKAA++QRAALSAALGGRVDV +M+TISGVKATYLLAV+FLEIIRFSS
Sbjct: 781  LHNPGSRRGSGNEKAAVSQRAALSAALGGRVDVGSMNTISGVKATYLLAVAFLEIIRFSS 840

Query: 841  NGGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDT 900
            NGG++NGG+++NASRSAF CVFEYLK+PNL+PAV QCL AIVHRAFETAV WLEDRIS+T
Sbjct: 841  NGGVINGGTSLNASRSAFSCVFEYLKSPNLMPAVFQCLMAIVHRAFETAVLWLEDRISET 900

Query: 901  GNEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFS 960
            G +AE R+STL AH C+LIKSMSQR+EH+RD+AVNLL QLRDKFPQV+WNSSC DSLLFS
Sbjct: 901  GMKAEYRESTLSAHACFLIKSMSQREEHIRDVAVNLLAQLRDKFPQVLWNSSCFDSLLFS 960

Query: 961  MHNDAPSTVVSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQ 1020
            MHND+PS VV+DP+W+VTVRSLYQ++VREWI+KSLS+APCT QGLLQ+KLCKANTWQRAQ
Sbjct: 961  MHNDSPSVVVNDPSWMVTVRSLYQKIVREWIIKSLSHAPCTTQGLLQDKLCKANTWQRAQ 1020

Query: 1021 HTPDVISLLSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGM 1080
             T DVISLLSEIRIGT KNE WTGIQTANIPAV+ AAAAASGA+ KLTEAFNLEVLSTG+
Sbjct: 1021 PTTDVISLLSEIRIGTMKNECWTGIQTANIPAVMAAAAAASGANFKLTEAFNLEVLSTGI 1080

Query: 1081 VSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDS 1140
            VSATVKCNHAGEIAGMRRLYNSIGGFQ+G   + G G G GLQRLI+GA PQQP  EDDS
Sbjct: 1081 VSATVKCNHAGEIAGMRRLYNSIGGFQSGTT-TGGFGIGIGLQRLISGAFPQQPLAEDDS 1140

Query: 1141 FNGILIMKFVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQL 1200
            FNG+L+ KFV+ LQQFV+ +EKG  +DKL FRETCSQATALLLSNL S+SK+NIEGF+QL
Sbjct: 1141 FNGMLLAKFVRMLQQFVNISEKGGDVDKLHFRETCSQATALLLSNLGSDSKSNIEGFSQL 1200

Query: 1201 IRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASD 1260
            +RLLCWCPAYISTPDA+ETGVFIWTWLVSAAPELGS VLAELVDAWLWTIDTKRGLFASD
Sbjct: 1201 LRLLCWCPAYISTPDAMETGVFIWTWLVSAAPELGSLVLAELVDAWLWTIDTKRGLFASD 1260

Query: 1261 AS-----------LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFG 1320
                         L  GEPE QPEIDPVEQI+AHRIWLGFFIDRFEVVRHNSVEQLLL G
Sbjct: 1261 VKYSGPAAKLRPHLSPGEPEGQPEIDPVEQIVAHRIWLGFFIDRFEVVRHNSVEQLLLLG 1320

Query: 1321 RLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRAS 1380
            R+LQG+TK PWNFSRHPAA G+FFT+MLLGLKFCSCQ+QGNL NFKTGL+LLEDRIYRAS
Sbjct: 1321 RMLQGTTKLPWNFSRHPAAAGTFFTVMLLGLKFCSCQSQGNLHNFKTGLQLLEDRIYRAS 1380

Query: 1381 LGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLIDSN 1440
            LGWFA+E EWYD+ ++NF+QSEAQSVS+F+HYLS+ER +  QSD+K+RGRENG +L+D+N
Sbjct: 1381 LGWFAYEAEWYDINNINFSQSEAQSVSVFVHYLSNERVDGGQSDSKVRGRENGSTLVDAN 1440

Query: 1441 YQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPKLTAEKWI 1500
            +QYHPVWG +ENYAVGREKR+QLLLMLCQHEADRLEVWAQP N K+ST SR K+++EKWI
Sbjct: 1441 HQYHPVWGQMENYAVGREKRKQLLLMLCQHEADRLEVWAQPTNSKDSTSSRSKISSEKWI 1500

Query: 1501 EHARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDE 1560
            E+ARTAFSVDPRIA S+ SRFPTN FL+ E+  LVQ +ILDIR IPEALPYFVTPKAVDE
Sbjct: 1501 EYARTAFSVDPRIALSLASRFPTNMFLKAEVTHLVQSNILDIRGIPEALPYFVTPKAVDE 1560

Query: 1561 NSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQAL 1620
            NS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQ+L
Sbjct: 1561 NSALLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQSL 1620

Query: 1621 RYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQ 1680
            RYD+ RLVEGYLLRAAQRSD+FAHILIWHLQGET +P+SGK+  SGKN SF  LLP+VR 
Sbjct: 1621 RYDDARLVEGYLLRAAQRSDLFAHILIWHLQGETCVPESGKEAISGKNTSFYELLPLVRD 1680

Query: 1681 HIIDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLP 1740
             IIDGF+PKAL+LFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIE++GEDLYLP
Sbjct: 1681 RIIDGFSPKALELFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEVDGEDLYLP 1740

Query: 1741 TATNKLVRGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVL 1800
            TA +KLVRGIQVDSGIPLQSAAKVPIM+TFNVVDR GD N++KPQACIFKVGDDCRQDVL
Sbjct: 1741 TAPSKLVRGIQVDSGIPLQSAAKVPIMITFNVVDRYGDRNDVKPQACIFKVGDDCRQDVL 1800

Query: 1801 ALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIF 1860
            ALQVI+LL DIF+AVG+NLYL+PYGVLPTGP RGIIEVVPNTRSRSQMGETTDGGLYEIF
Sbjct: 1801 ALQVIALLSDIFKAVGINLYLYPYGVLPTGPERGIIEVVPNTRSRSQMGETTDGGLYEIF 1860

Query: 1861 QQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFIL 1920
            QQD+GPVG+P+FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFIL
Sbjct: 1861 QQDHGPVGTPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFIL 1920

Query: 1921 ETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINT 1980
            ETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR+MDGIINT
Sbjct: 1921 ETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSDTWHQFVSLCVKGYLAARRYMDGIINT 1980

Query: 1981 VLLMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYL 1990
            VLLMLDSGLPCFSRGDPIGNLRKRFHPEM++REAANFMI VCTDAYNKWTTAGYDLIQYL
Sbjct: 1981 VLLMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIHVCTDAYNKWTTAGYDLIQYL 2040

BLAST of PI0013678 vs. NCBI nr
Match: XP_008447043.1 (PREDICTED: phosphatidylinositol 4-kinase alpha 1-like [Cucumis melo])

HSP 1 Score: 3820.8 bits (9907), Expect = 0.0e+00
Identity = 1946/2037 (95.53%), Postives = 1960/2037 (96.22%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            MDALIELCDLIAQNPA FSEKLSWICSRCPPSEAILAGSP ISRSQ+NAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPTISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            PDSVGLRPKS VLEFLRSIPLSFSLSFWPQSYG+DAIASFFNEFLNYTSKACELSTDFAT
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGHDAIASFFNEFLNYTSKACELSTDFAT 120

Query: 121  EVSGFSSEVVF-----LSCGS----------VESFLPIIPSDADKLVSCILDRFFISEAA 180
            EVSGFSSEVV       S GS           +SFLPIIPSDADKLVSCILDRF ISEA 
Sbjct: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALTKSFLPIIPSDADKLVSCILDRFLISEAI 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            P TPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PETPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGA----------------------GDCLQ 300
            SSRGSGMMNGNSILWKSGLEQLGMNLGF+EGG                           +
Sbjct: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFSEGGGVAFVRQQVALFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINTKLLVYQ 360
            LMTHILDNSSFDARLWEQM+ LAKKQLQTLPTFLKIKKRDWSEQGSVLK+RINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMKTLAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660
            TVAPEATTERVEILPAGFLHIANGLKS KLRLEYRHRLLSLCSDVGLAAESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLHIANGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRD 900
            SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAE RD
Sbjct: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEARD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLR+KFPQVMWNSSCLDSLLFSMHNDAPST
Sbjct: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLREKFPQVMWNSSCLDSLLFSMHNDAPST 960

Query: 961  VVSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020
            VVSDPAW+VTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL
Sbjct: 961  VVSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGTSKNEHWTG QTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTSKNEHWTGTQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKG GLDKLKFRETCSQATALLLSNL SESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLDSESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDAS------ 1260
            AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASD        
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDIKYSGPAA 1260

Query: 1261 -----LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
                 L  GEPE+QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLIDSNYQYHPVWG 1440
            EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAK+RGRENGISLIDSN  YHPVWG
Sbjct: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKIRGRENGISLIDSNDHYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKLTAEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPYRPKLTAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680
            GYLLRAA+RSDIFAHILIWHLQGETS PDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK
Sbjct: 1621 GYLLRAAKRSDIFAHILIWHLQGETSSPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740
            ALDLF+REFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEM+GEDLYLPTATNKLVRG
Sbjct: 1681 ALDLFRREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMDGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1990
            PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2037

BLAST of PI0013678 vs. NCBI nr
Match: XP_011659072.1 (phosphatidylinositol 4-kinase alpha 1 [Cucumis sativus] >KGN44615.2 hypothetical protein Csa_016276 [Cucumis sativus])

HSP 1 Score: 3795.7 bits (9842), Expect = 0.0e+00
Identity = 1939/2037 (95.19%), Postives = 1952/2037 (95.83%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            MDALIELCDLIAQ+PA FSEKLSWICSRCPPSEAILAGSPAISRSQ+NAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQSPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            PDSVGLRPKS VLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120

Query: 121  EVSGFSSEVVF-----LSCGSV----------ESFLPIIPSDADKLVSCILDRFFISEAA 180
            EVSGFSSEVV       S GS           +SFLPIIPSDADKLVSCILDRF ISEAA
Sbjct: 121  EVSGFSSEVVLSAINDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGA----------------------GDCLQ 300
            SSRGSGMMNGNSILW+SGLEQ      F+EGG                           +
Sbjct: 241  SSRGSGMMNGNSILWRSGLEQ------FSEGGGVAFVRQQVALFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINTKLLVYQ 360
            LMTHILDNSSFD RLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLK+RINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDGRLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAA+ACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAADACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660
            TVAPEATTERVEILPAGFLHIANGLKS KLRLEYRHRLLSLCSDVGLAAESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLHIANGLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKS LQTKSVSTMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSHLQTKSVSTMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQ IARGTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQLIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRD 900
            SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAV WLEDRISDTGNEAEVRD
Sbjct: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISDTGNEAEVRD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST
Sbjct: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960

Query: 961  VVSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020
            VV+DPAW+VTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL
Sbjct: 961  VVTDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVA   GLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVA---GLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKG GLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDAS------ 1260
            AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASD        
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDVKYSGPAA 1260

Query: 1261 -----LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
                 L  GEPE+QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 MLRPHLSPGEPEMQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLIDSNYQYHPVWG 1440
            EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSL SDAKMRGRENGISLID N  YHPVWG
Sbjct: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLHSDAKMRGRENGISLIDLNDHYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680
            GYLLRAA+RSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK
Sbjct: 1621 GYLLRAAKRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740
            ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIR ELEKIEMEGEDLYLPTATNKLVRG
Sbjct: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRSELEKIEMEGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            P+FEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PSFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYL ARRHMDGIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLTARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1990
            PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2028

BLAST of PI0013678 vs. NCBI nr
Match: XP_038887703.1 (phosphatidylinositol 4-kinase alpha 1-like [Benincasa hispida])

HSP 1 Score: 3791.5 bits (9831), Expect = 0.0e+00
Identity = 1929/2037 (94.70%), Postives = 1956/2037 (96.02%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            MDALIELCDLIAQNPA FSEKLSWICSRCPPSEAILAGSPAISRSQ+NAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            PDSVGLRPKS VLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAM 120

Query: 121  EVSGFSSEVVFLSC-----GSV----------ESFLPIIPSDADKLVSCILDRFFISEAA 180
            E+SGFSSEVV  +      GS           +SFLPIIPSDADKLVSCILDRF ISEAA
Sbjct: 121  EISGFSSEVVLSAINDCCEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDRFLISEAA 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPREHNQANSE SSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREHNQANSEASSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGA----------------------GDCLQ 300
            SSRGSGMMNGNSILWKSGLEQLGMN GF+EGG                           +
Sbjct: 241  SSRGSGMMNGNSILWKSGLEQLGMNQGFSEGGGVAFVRQQVASFEDESIENLEKQEIAFK 300

Query: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINTKLLVYQ 360
            LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLK+RINTKLLVYQ
Sbjct: 301  LMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQ 360

Query: 361  AAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420
            AAARLKMKTV SLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL
Sbjct: 361  AAARLKMKTVVSLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGL 420

Query: 421  AQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDR 480
            +QIAV+RGGQPLRVLLIRLKPLVLTVC QADTWG NQGAMFESVLTTCCEIIESCWTKDR
Sbjct: 421  SQIAVSRGGQPLRVLLIRLKPLVLTVCAQADTWG-NQGAMFESVLTTCCEIIESCWTKDR 480

Query: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540
            APVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKMTVAVNKSEIVDMILPL
Sbjct: 481  APVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMTVAVNKSEIVDMILPL 540

Query: 541  FIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600
            FIESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVLMTRSYLSKLSSIGSSESR
Sbjct: 541  FIESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLMTRSYLSKLSSIGSSESR 600

Query: 601  TVAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660
            TVAPEATTERVEILPAGFL IAN LKS KLRLEYRHRLLSLCSDVGLAAESKSGRSGADF
Sbjct: 601  TVAPEATTERVEILPAGFLLIANSLKSAKLRLEYRHRLLSLCSDVGLAAESKSGRSGADF 660

Query: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720
            LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN
Sbjct: 661  LGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLN 720

Query: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780
            SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR
Sbjct: 721  SVGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRR 780

Query: 781  GSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840
            GSGNEKAA+AQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG
Sbjct: 781  GSGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGG 840

Query: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRD 900
            SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAV WLEDRIS+TGNEAEVRD
Sbjct: 841  SNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVLWLEDRISETGNEAEVRD 900

Query: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960
            STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST
Sbjct: 901  STLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPST 960

Query: 961  VVSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISL 1020
            V+SDPAW+VTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQR QHTPDVISL
Sbjct: 961  VISDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRGQHTPDVISL 1020

Query: 1021 LSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080
            LSEIRIGTSKN++WTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN
Sbjct: 1021 LSEIRIGTSKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCN 1080

Query: 1081 HAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMK 1140
            HAGEIAGMRRLYNSIGGFQTGVAG TGLGFGQGLQRLITGA+PQQPQNEDDSFNGILIMK
Sbjct: 1081 HAGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGAVPQQPQNEDDSFNGILIMK 1140

Query: 1141 FVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200
            FVQSLQQFVSGAEKG GLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP
Sbjct: 1141 FVQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCP 1200

Query: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDAS------ 1260
            AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFAS+        
Sbjct: 1201 AYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAA 1260

Query: 1261 -----LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320
                 L  GEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK
Sbjct: 1261 KLRPHLSPGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTK 1320

Query: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEP 1380
            PPWNFSRHPAATGSFFTLMLLGLKFCSCQ+QGNLQNFKTGLELLEDR+YRASLGWFAHEP
Sbjct: 1321 PPWNFSRHPAATGSFFTLMLLGLKFCSCQSQGNLQNFKTGLELLEDRLYRASLGWFAHEP 1380

Query: 1381 EWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLIDSNYQYHPVWG 1440
            EWYDVKHVNFAQS+AQSVSIFLHYLSSERGNSLQSDAK+RGREN ISL+DSN QYHPVWG
Sbjct: 1381 EWYDVKHVNFAQSQAQSVSIFLHYLSSERGNSLQSDAKIRGRENSISLVDSNDQYHPVWG 1440

Query: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSV 1500
            HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKL+AEKWIEHARTAFSV
Sbjct: 1441 HLENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLSAEKWIEHARTAFSV 1500

Query: 1501 DPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLP 1560
            DPRIAFSMVSRFPTNA LRVEMNQLVQLHILDIR+IPEALPYFVTPKAVDENSELLRQLP
Sbjct: 1501 DPRIAFSMVSRFPTNASLRVEMNQLVQLHILDIRTIPEALPYFVTPKAVDENSELLRQLP 1560

Query: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620
            HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE
Sbjct: 1561 HWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVE 1620

Query: 1621 GYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680
            GYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK
Sbjct: 1621 GYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPK 1680

Query: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740
            ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG
Sbjct: 1681 ALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800
            IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLR 1800

Query: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920
            P+FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR
Sbjct: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1990
            PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of PI0013678 vs. NCBI nr
Match: KAG7011695.1 (Phosphatidylinositol 4-kinase alpha 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 3732.6 bits (9678), Expect = 0.0e+00
Identity = 1894/2036 (93.03%), Postives = 1937/2036 (95.14%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            MDALIELCDLIAQNPA FSEKLSWICSRCPPSEAILAGSPAISRSQ+NAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            PDSVGLRPKS VLEFLRSIPLSFSL+FWPQS+GNDAIASFF EFLNYTSKACELSTDFA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLAFWPQSFGNDAIASFFTEFLNYTSKACELSTDFAM 120

Query: 121  EVSGFSSEVVF-----LSCGSV----------ESFLPIIPSDADKLVSCILDRFFISEAA 180
            EVSGF+SEVV       S GS           +SFLPIIPSDADKLVSCILD F ISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  QANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGAGDCLQ---------------------L 300
            SSRGSGMMNGNSILWKSGL+QLGMNLG +EGG     Q                     L
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSEGGVAFVRQQVAFFEDESIENLEKQEIAFKL 300

Query: 301  MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINTKLLVYQA 360
            MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLK+RINTKLLVYQA
Sbjct: 301  MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQA 360

Query: 361  AARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA 420
            AARLKM TVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA
Sbjct: 361  AARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA 420

Query: 421  QIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDRA 480
            QIAV+RGGQPLRVLLIRLKPL LTVC QADTWG+NQGAMFESVLTTCCEIIESCWTKDRA
Sbjct: 421  QIAVSRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDRA 480

Query: 481  PVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPLF 540
            PVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKM+ AVNKSEIV+MILPLF
Sbjct: 481  PVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPLF 540

Query: 541  IESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESRT 600
            IESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVL+TRSYLSKLSSIGSSESRT
Sbjct: 541  IESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESRT 600

Query: 601  VAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKSGRSGADFL 660
            VAPEATTERVEILPAGFLHIA+GLKS KLRLEYRHRLL LCSDVGLAAESKSGRSGADFL
Sbjct: 601  VAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADFL 660

Query: 661  GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLNS 720
            GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVS+MLNS
Sbjct: 661  GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLNS 720

Query: 721  VGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRRG 780
            VGSTAIALQAVSGPYLWNTQWSSAVQFIA+GTPPLVVSSVKWLEDELELNALHNPGSRRG
Sbjct: 721  VGSTAIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRRG 780

Query: 781  SGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS 840
            SGNEKAA+AQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS
Sbjct: 781  SGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS 840

Query: 841  NVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRDS 900
            NVNA+RSAF CVFEYLKTPNLLPAV+QCLTAIVHRAFETAVSWLEDRIS+TGNEAEVRDS
Sbjct: 841  NVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVRDS 900

Query: 901  TLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPSTV 960
            TL+AHTCYLIK MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA STV
Sbjct: 901  TLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASSTV 960

Query: 961  VSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL 1020
            VSDPAW+VTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL
Sbjct: 961  VSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL 1020

Query: 1021 SEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH 1080
            SEIRIGT KN++WTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH
Sbjct: 1021 SEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH 1080

Query: 1081 AGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMKF 1140
            AGEIAGMRRLYNSIGGFQTGVAG TGLGFGQGLQRLITG LPQQPQNEDDSFNGILIMKF
Sbjct: 1081 AGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIMKF 1140

Query: 1141 VQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA 1200
            VQSLQQFVSGAEKG GLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA
Sbjct: 1141 VQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA 1200

Query: 1201 YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDAS------- 1260
            YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFAS+         
Sbjct: 1201 YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAAK 1260

Query: 1261 ----LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP 1320
                L  GEPE QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP
Sbjct: 1261 LRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP 1320

Query: 1321 PWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEPE 1380
            PWNFSRHPAATGSFFTLMLLGLKF SCQ+QGNLQNFKTGLELLEDRIYRASLGWFAHEPE
Sbjct: 1321 PWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHEPE 1380

Query: 1381 WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLIDSNYQYHPVWGH 1440
            WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSD K RGRENGISL+DSN QYHPVWGH
Sbjct: 1381 WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVWGH 1440

Query: 1441 LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSVD 1500
            LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKL+AEKWIEHARTAFSVD
Sbjct: 1441 LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPVRPKLSAEKWIEHARTAFSVD 1500

Query: 1501 PRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPH 1560
            PRIAFSMVSRFPTN FLR E+ QLVQLHILDIRSIPEALPYF+TPKAVDENSELLRQLPH
Sbjct: 1501 PRIAFSMVSRFPTNTFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVDENSELLRQLPH 1560

Query: 1561 WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEG 1620
            WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLVEG
Sbjct: 1561 WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLVEG 1620

Query: 1621 YLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPKA 1680
            YLLRAAQRSDIFAHILIWHLQGETS+PDSGKD NSGKN SFLALLP+VR HIIDGFTPKA
Sbjct: 1621 YLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTPKA 1680

Query: 1681 LDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI 1740
             DLF+REFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI
Sbjct: 1681 RDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI 1740

Query: 1741 QVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLRD 1800
            QVDSGIPLQSAAKVPIM+TFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVI+LLRD
Sbjct: 1741 QVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALLRD 1800

Query: 1801 IFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860
            IFQAVGLNLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP
Sbjct: 1801 IFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860

Query: 1861 NFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF 1920
            +FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF
Sbjct: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF 1920

Query: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP 1980
            ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP
Sbjct: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP 1980

Query: 1981 CFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1990
            CFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of PI0013678 vs. NCBI nr
Match: KAG6572023.1 (Phosphatidylinositol 4-kinase alpha 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3732.2 bits (9677), Expect = 0.0e+00
Identity = 1894/2036 (93.03%), Postives = 1936/2036 (95.09%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            MDALIELCDLIAQNPA FSEKLSWICSRCPPSEAILAGSPAISRSQ+NAVLAVARLLSKC
Sbjct: 1    MDALIELCDLIAQNPALFSEKLSWICSRCPPSEAILAGSPAISRSQLNAVLAVARLLSKC 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            PDSVGLRPKS VLEFLRSIPLSFSL+FWPQS+GNDAIASFF EFLNYTSKACELSTDFA 
Sbjct: 61   PDSVGLRPKSVVLEFLRSIPLSFSLAFWPQSFGNDAIASFFTEFLNYTSKACELSTDFAM 120

Query: 121  EVSGFSSEVVF-----LSCGSV----------ESFLPIIPSDADKLVSCILDRFFISEAA 180
            EVSGF+SEVV       S GS           +SFLPIIPSDADKLVSCILD F ISE  
Sbjct: 121  EVSGFTSEVVLSAISDCSEGSAISRAFLVALSKSFLPIIPSDADKLVSCILDLFLISEVV 180

Query: 181  PGTPREHNQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240
            PGTPRE  QANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA
Sbjct: 181  PGTPREQIQANSEPSSSQSSPLSVSHQPSNGGLSPGNENGQVSGSLSSGASRITDDATSA 240

Query: 241  SSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGAGDCLQ---------------------L 300
            SSRGSGMMNGNSILWKSGL+QLGMNLG +EGG     Q                     L
Sbjct: 241  SSRGSGMMNGNSILWKSGLDQLGMNLGTSEGGVAFVRQQVAFFEDESIENLEKQEIAFKL 300

Query: 301  MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKSRINTKLLVYQA 360
            MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLK+RINTKLLVYQA
Sbjct: 301  MTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSVLKARINTKLLVYQA 360

Query: 361  AARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA 420
            AARLKM TVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA
Sbjct: 361  AARLKMTTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLRICEELFGSLLTGLA 420

Query: 421  QIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTTCCEIIESCWTKDRA 480
            QIAV RGGQPLRVLLIRLKPL LTVC QADTWG+NQGAMFESVLTTCCEIIESCWTKDRA
Sbjct: 421  QIAVTRGGQPLRVLLIRLKPLALTVCAQADTWGSNQGAMFESVLTTCCEIIESCWTKDRA 480

Query: 481  PVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVAVNKSEIVDMILPLF 540
            PVDTFIMGLATSIRDRNDSEEQDDKEKQ VPMQLNVIRLLAKM+ AVNKSEIV+MILPLF
Sbjct: 481  PVDTFIMGLATSIRDRNDSEEQDDKEKQAVPMQLNVIRLLAKMSAAVNKSEIVEMILPLF 540

Query: 541  IESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSYLSKLSSIGSSESRT 600
            IESLEEGDASTPGLLRLQLLDAVSRMA+LGFEKSYRETIVL+TRSYLSKLSSIGSSESRT
Sbjct: 541  IESLEEGDASTPGLLRLQLLDAVSRMASLGFEKSYRETIVLLTRSYLSKLSSIGSSESRT 600

Query: 601  VAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGLAAESKSGRSGADFL 660
            VAPEATTERVEILPAGFLHIA+GLKS KLRLEYRHRLL LCSDVGLAAESKSGRSGADFL
Sbjct: 601  VAPEATTERVEILPAGFLHIASGLKSAKLRLEYRHRLLCLCSDVGLAAESKSGRSGADFL 660

Query: 661  GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSTMLNS 720
            GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVS+MLNS
Sbjct: 661  GPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQKSQLQTKSVSSMLNS 720

Query: 721  VGSTAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLEDELELNALHNPGSRRG 780
            VGSTAIALQAVSGPYLWNTQWSSAVQFIA+GTPPLVVSSVKWLEDELELNALHNPGSRRG
Sbjct: 721  VGSTAIALQAVSGPYLWNTQWSSAVQFIAQGTPPLVVSSVKWLEDELELNALHNPGSRRG 780

Query: 781  SGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS 840
            SGNEKAA+AQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS
Sbjct: 781  SGNEKAAVAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFLEIIRFSSNGGILNGGS 840

Query: 841  NVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWLEDRISDTGNEAEVRDS 900
            NVNA+RSAF CVFEYLKTPNLLPAV+QCLTAIVHRAFETAVSWLEDRIS+TGNEAEVRDS
Sbjct: 841  NVNAARSAFSCVFEYLKTPNLLPAVAQCLTAIVHRAFETAVSWLEDRISETGNEAEVRDS 900

Query: 901  TLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSCLDSLLFSMHNDAPSTV 960
            TL+AHTCYLIK MSQRDEHVRDI+VNLLTQLRDKFPQVMWNS+CLDSLLFSMHNDA STV
Sbjct: 901  TLYAHTCYLIKCMSQRDEHVRDISVNLLTQLRDKFPQVMWNSACLDSLLFSMHNDASSTV 960

Query: 961  VSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL 1020
            VSDPAW+VTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL
Sbjct: 961  VSDPAWVVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKANTWQRAQHTPDVISLL 1020

Query: 1021 SEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH 1080
            SEIRIGT KN++WTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH
Sbjct: 1021 SEIRIGTGKNDYWTGIQTANIPAVITAAAAASGADLKLTEAFNLEVLSTGMVSATVKCNH 1080

Query: 1081 AGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQPQNEDDSFNGILIMKF 1140
            AGEIAGMRRLYNSIGGFQTGVAG TGLGFGQGLQRLITG LPQQPQNEDDSFNGILIMKF
Sbjct: 1081 AGEIAGMRRLYNSIGGFQTGVAGPTGLGFGQGLQRLITGTLPQQPQNEDDSFNGILIMKF 1140

Query: 1141 VQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA 1200
            VQSLQQFVSGAEKG GLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA
Sbjct: 1141 VQSLQQFVSGAEKGCGLDKLKFRETCSQATALLLSNLASESKTNIEGFAQLIRLLCWCPA 1200

Query: 1201 YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASDAS------- 1260
            YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFAS+         
Sbjct: 1201 YISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTKRGLFASEVKYSGPAAK 1260

Query: 1261 ----LISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP 1320
                L  GEPE QPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP
Sbjct: 1261 LRPHLSPGEPESQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSVEQLLLFGRLLQGSTKP 1320

Query: 1321 PWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLEDRIYRASLGWFAHEPE 1380
            PWNFSRHPAATGSFFTLMLLGLKF SCQ+QGNLQNFKTGLELLEDRIYRASLGWFAHEPE
Sbjct: 1321 PWNFSRHPAATGSFFTLMLLGLKFYSCQSQGNLQNFKTGLELLEDRIYRASLGWFAHEPE 1380

Query: 1381 WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENGISLIDSNYQYHPVWGH 1440
            WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSD K RGRENGISL+DSN QYHPVWGH
Sbjct: 1381 WYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDTKTRGRENGISLVDSNDQYHPVWGH 1440

Query: 1441 LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPSRPKLTAEKWIEHARTAFSVD 1500
            LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTP RPKL+AEKWIEHARTAFSVD
Sbjct: 1441 LENYAVGREKRRQLLLMLCQHEADRLEVWAQPNIKESTPVRPKLSAEKWIEHARTAFSVD 1500

Query: 1501 PRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFVTPKAVDENSELLRQLPH 1560
            PRIAFSMVSRFPTN FLR E+ QLVQLHILDIRSIPEALPYF+TPKAVDENSELLRQLPH
Sbjct: 1501 PRIAFSMVSRFPTNTFLRAEVKQLVQLHILDIRSIPEALPYFITPKAVDENSELLRQLPH 1560

Query: 1561 WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFMPQLVQALRYDEGRLVEG 1620
            WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE VTFFMPQLVQALRYDEGRLVEG
Sbjct: 1561 WAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPENVTFFMPQLVQALRYDEGRLVEG 1620

Query: 1621 YLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGFTPKA 1680
            YLLRAAQRSDIFAHILIWHLQGETS+PDSGKD NSGKN SFLALLP+VR HIIDGFTPKA
Sbjct: 1621 YLLRAAQRSDIFAHILIWHLQGETSVPDSGKDANSGKNASFLALLPIVRHHIIDGFTPKA 1680

Query: 1681 LDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI 1740
             DLF+REFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI
Sbjct: 1681 RDLFEREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKLVRGI 1740

Query: 1741 QVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVISLLRD 1800
            QVDSGIPLQSAAKVPIM+TFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVI+LLRD
Sbjct: 1741 QVDSGIPLQSAAKVPIMITFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIALLRD 1800

Query: 1801 IFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860
            IFQAVGLNLYLFPYGVLPTGPG+GIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP
Sbjct: 1801 IFQAVGLNLYLFPYGVLPTGPGKGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860

Query: 1861 NFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF 1920
            +FEAARENFI+SSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF
Sbjct: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGGNMRF 1920

Query: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP 1980
            ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP
Sbjct: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLDSGLP 1980

Query: 1981 CFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1990
            CFSRGDPIGNLRKRFHPEM++REAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 2036

BLAST of PI0013678 vs. TAIR 10
Match: AT1G49340.2 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 2899.0 bits (7514), Expect = 0.0e+00
Identity = 1486/2053 (72.38%), Postives = 1702/2053 (82.90%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            M+AL ELCD+IA+NP  FSEKL+WIC RCP +E +LA SP +SRS +NAVLAVAR++SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            P+S+  R KS V EFL +IP SF  SFWP S+ +  I+SF+ +FL+Y S A +LS +F T
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EVSGFSSEVVFLSCGS-------------------VESFLPIIPSDADKLVSCILDRFFI 180
            EV+ F+ EVV  +                       + F  I+ SD DKL++ +LD+F +
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  SEAAPGTPREHNQANS-----EPSSSQSSPLSVSHQPSNGG--LSPGNENGQVSGSLSSG 240
            +  AP +P+E  Q NS     + SSSQ SP+S +  PS      SPG+E      +LSS 
Sbjct: 181  NR-APASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDEVASHGSNLSS- 240

Query: 241  ASRITDDATSASSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGAGD-------------- 300
                        S  S +MNG SI+WKSG++QL  + GF+EG  G               
Sbjct: 241  -----------KSSSSVVMNGGSIVWKSGVDQL--SFGFSEGSGGANPVFRQQVASFEDE 300

Query: 301  ----------CLQLMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSV 360
                        +L+THILD    D++L +Q+R +AK+QLQ++  FLK +KRDW+EQG V
Sbjct: 301  SIESLEKQEIAFRLITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQV 360

Query: 361  LKSRINTKLLVYQAAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLR 420
            LK+R+N KL VYQAAA++K+K++ SL+ DGK +K+L+ ET ALL+DAA+ACL SVWRK++
Sbjct: 361  LKTRVNAKLSVYQAAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMK 420

Query: 421  ICEELFGSLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTT 480
             CEELF SLL+G+A+IAVARGGQPLRVLLIRLKPLVL VC   D     QGAM ES+  T
Sbjct: 421  ACEELFDSLLSGIAKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKT 480

Query: 481  CCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVA 540
             C IIES W KDRAPVD FIMGLA+SIR+RND EEQ D+EKQ   +QLNVIRLLA + VA
Sbjct: 481  SCVIIESAWAKDRAPVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVA 540

Query: 541  VNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSY 600
            V K E+ DMILPLFIESLEEGDASTP  LRLQLLDAVSR+ATLGF+KSYRET+VLMTRSY
Sbjct: 541  VKKPEVADMILPLFIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSY 600

Query: 601  LSKLSSIGSSESRTVAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGL 660
            LSKLSS+GS ES+T APEATTERVE LPAGFL IA+GL  TKLR +YRHRLLSLCSDVGL
Sbjct: 601  LSKLSSVGSVESKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGL 660

Query: 661  AAESKSGRSGADFLGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQK 720
            AAESKSG SG DFLGPLLPAVAEICSDFDPTM++EPSLLKLFRNLWFYIALFGLAPPI K
Sbjct: 661  AAESKSGGSGVDFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVK 720

Query: 721  SQL-QTKSVSTMLNSVGS-TAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLE 780
            +     KS S  +NSVGS +A ALQAV GPY+W+ QW+ AVQ IA+GTPPLVVSSVKWLE
Sbjct: 721  TPTPPLKSTSNSVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLE 780

Query: 781  DELELNALHNPGSRRGSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFL 840
            DELELNALHNPGSRRG+GNEK A  QR ALS ALGGRVDVAAM+TISGVKATYLLAV+FL
Sbjct: 781  DELELNALHNPGSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFL 840

Query: 841  EIIRFSSNGGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWL 900
            EIIRF SNGGILNG S+V+ASRSAF CVFEYLKTPNL PAVSQCLTAIVHRAFETAVSWL
Sbjct: 841  EIIRFISNGGILNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWL 900

Query: 901  EDRISDTGNEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSC 960
            EDRIS TG +A  R+ T +AH C+LIKSMSQRDEHVRDI+VNLLTQLRDKFPQV+W+SSC
Sbjct: 901  EDRISLTGKDARNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSC 960

Query: 961  LDSLLFSMHNDAPSTVVSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKA 1020
            LDSLLFS+H++ PSTVV+DPAW   VRSLYQ+VVREWI+ SLSYAPCT QGLLQ+KLCKA
Sbjct: 961  LDSLLFSVHDNTPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKA 1020

Query: 1021 NTWQRAQHTPDVISLLSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNL 1080
            NTWQRAQ T DV+SLLSEI+IGT KNE W+GI+TANIPAV+ AAAAASGA+LK++EAFNL
Sbjct: 1021 NTWQRAQTTTDVVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNL 1080

Query: 1081 EVLSTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQ 1140
            EVL TG+VSATVKCNHAGEIAGMRRLYNSIGGFQ   +GST  GFG GLQRLI+GA  Q 
Sbjct: 1081 EVLGTGVVSATVKCNHAGEIAGMRRLYNSIGGFQ---SGSTPSGFGGGLQRLISGAFSQA 1140

Query: 1141 PQNEDDSFNGILIMKFVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTN 1200
            PQ EDDSFN +LI +FV+ LQQFV+ AEKG  ++K +FRETCSQATALLLSNL  ESKTN
Sbjct: 1141 PQPEDDSFNEMLIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTN 1200

Query: 1201 IEGFAQLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTK 1260
            +EGF+QL+RLLCWCPAYISTPDA+ETG+FIWTWLVSAAP+L S VLAELVDAW+WTIDTK
Sbjct: 1201 VEGFSQLLRLLCWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTK 1260

Query: 1261 RGLFASDA-----------SLISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSV 1320
            RGLFASD             L  GEPE  PE DPV+QI+AHR+WLGF IDRFEVVRHNS 
Sbjct: 1261 RGLFASDVRYSGPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSA 1320

Query: 1321 EQLLLFGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLE 1380
            EQLLL GR+LQ ST   W F+RHPAA G+FF+LMLLGLKFCSCQ QGN+Q F++GL+LLE
Sbjct: 1321 EQLLLLGRMLQRSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLE 1380

Query: 1381 DRIYRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENG 1440
            DRIYR SLGWFAH+PEWYDV   NF  SEA SVS+F+H+LS+E   S QSD+K + RE+G
Sbjct: 1381 DRIYRTSLGWFAHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESG 1440

Query: 1441 ISLIDSNYQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPK 1500
             +LID   QYHPVWG ++NY +G+EKR+QLLLMLCQHEADRL+VWAQP + K+S  SR K
Sbjct: 1441 -NLIDVTDQYHPVWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLK 1500

Query: 1501 LTAEKWIEHARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFV 1560
            +++EKW E+A+TAFSVDPRIA S+ SRFP NA ++ E+ QLVQ +I+D+R+IPEALPYFV
Sbjct: 1501 ISSEKWTEYAKTAFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFV 1560

Query: 1561 TPKAVDENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFM 1620
            TPK V+ENS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE+VTFFM
Sbjct: 1561 TPKNVEENSVLLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFM 1620

Query: 1621 PQLVQALRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLA 1680
            PQLVQ+LRYD+GRLVEGYLLRA QRSDIFAHILIWHLQGE  + ++ KD +  KN +F  
Sbjct: 1621 PQLVQSLRYDDGRLVEGYLLRATQRSDIFAHILIWHLQGE-DVQETPKDGSIDKNAAFQE 1680

Query: 1681 LLPVVRQHIIDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEME 1740
            +LP VRQHIIDGF+P ALD+F REFDFFDKVTSISGVLFPLPK+ERRAGIRRELEKIEM+
Sbjct: 1681 ILPQVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIEMQ 1740

Query: 1741 GEDLYLPTATNKLVRGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGD 1800
            G+DLYLPTA NKLVRGI+VDSGIPLQSAAKVPIM+TFNV+DRDGD +++KPQACIFKVGD
Sbjct: 1741 GDDLYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKVGD 1800

Query: 1801 DCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTD 1860
            DCRQDVLALQVISLLRDIFQA GLNLYLFPYGVLPTG  RGIIEVVPNTRSRSQMGETTD
Sbjct: 1801 DCRQDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGETTD 1860

Query: 1861 GGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVH 1920
            GGLYEIFQQDYGPVGS  FE ARENF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVH
Sbjct: 1861 GGLYEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVH 1920

Query: 1921 IDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRH 1980
            IDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR 
Sbjct: 1921 IDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQ 1980

Query: 1981 MDGIINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAG 1990
            MDGII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEM++REAA+FMI VCTDAYNKWTTAG
Sbjct: 1981 MDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAG 2028

BLAST of PI0013678 vs. TAIR 10
Match: AT1G49340.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 2899.0 bits (7514), Expect = 0.0e+00
Identity = 1486/2053 (72.38%), Postives = 1702/2053 (82.90%), Query Frame = 0

Query: 1    MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
            M+AL ELCD+IA+NP  FSEKL+WIC RCP +E +LA SP +SRS +NAVLAVAR++SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTDFAT 120
            P+S+  R KS V EFL +IP SF  SFWP S+ +  I+SF+ +FL+Y S A +LS +F T
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EVSGFSSEVVFLSCGS-------------------VESFLPIIPSDADKLVSCILDRFFI 180
            EV+ F+ EVV  +                       + F  I+ SD DKL++ +LD+F +
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  SEAAPGTPREHNQANS-----EPSSSQSSPLSVSHQPSNGG--LSPGNENGQVSGSLSSG 240
            +  AP +P+E  Q NS     + SSSQ SP+S +  PS      SPG+E      +LSS 
Sbjct: 181  NR-APASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDEVASHGSNLSS- 240

Query: 241  ASRITDDATSASSRGSGMMNGNSILWKSGLEQLGMNLGFNEGGAGD-------------- 300
                        S  S +MNG SI+WKSG++QL  + GF+EG  G               
Sbjct: 241  -----------KSSSSVVMNGGSIVWKSGVDQL--SFGFSEGSGGANPVFRQQVASFEDE 300

Query: 301  ----------CLQLMTHILDNSSFDARLWEQMRALAKKQLQTLPTFLKIKKRDWSEQGSV 360
                        +L+THILD    D++L +Q+R +AK+QLQ++  FLK +KRDW+EQG V
Sbjct: 301  SIESLEKQEIAFRLITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQV 360

Query: 361  LKSRINTKLLVYQAAARLKMKTVASLDFDGKPAKKLIFETFALLMDAAEACLLSVWRKLR 420
            LK+R+N KL VYQAAA++K+K++ SL+ DGK +K+L+ ET ALL+DAA+ACL SVWRK++
Sbjct: 361  LKTRVNAKLSVYQAAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMK 420

Query: 421  ICEELFGSLLTGLAQIAVARGGQPLRVLLIRLKPLVLTVCVQADTWGTNQGAMFESVLTT 480
             CEELF SLL+G+A+IAVARGGQPLRVLLIRLKPLVL VC   D     QGAM ES+  T
Sbjct: 421  ACEELFDSLLSGIAKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKT 480

Query: 481  CCEIIESCWTKDRAPVDTFIMGLATSIRDRNDSEEQDDKEKQGVPMQLNVIRLLAKMTVA 540
             C IIES W KDRAPVD FIMGLA+SIR+RND EEQ D+EKQ   +QLNVIRLLA + VA
Sbjct: 481  SCVIIESAWAKDRAPVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVA 540

Query: 541  VNKSEIVDMILPLFIESLEEGDASTPGLLRLQLLDAVSRMATLGFEKSYRETIVLMTRSY 600
            V K E+ DMILPLFIESLEEGDASTP  LRLQLLDAVSR+ATLGF+KSYRET+VLMTRSY
Sbjct: 541  VKKPEVADMILPLFIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSY 600

Query: 601  LSKLSSIGSSESRTVAPEATTERVEILPAGFLHIANGLKSTKLRLEYRHRLLSLCSDVGL 660
            LSKLSS+GS ES+T APEATTERVE LPAGFL IA+GL  TKLR +YRHRLLSLCSDVGL
Sbjct: 601  LSKLSSVGSVESKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGL 660

Query: 661  AAESKSGRSGADFLGPLLPAVAEICSDFDPTMNIEPSLLKLFRNLWFYIALFGLAPPIQK 720
            AAESKSG SG DFLGPLLPAVAEICSDFDPTM++EPSLLKLFRNLWFYIALFGLAPPI K
Sbjct: 661  AAESKSGGSGVDFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVK 720

Query: 721  SQL-QTKSVSTMLNSVGS-TAIALQAVSGPYLWNTQWSSAVQFIARGTPPLVVSSVKWLE 780
            +     KS S  +NSVGS +A ALQAV GPY+W+ QW+ AVQ IA+GTPPLVVSSVKWLE
Sbjct: 721  TPTPPLKSTSNSVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLE 780

Query: 781  DELELNALHNPGSRRGSGNEKAALAQRAALSAALGGRVDVAAMSTISGVKATYLLAVSFL 840
            DELELNALHNPGSRRG+GNEK A  QR ALS ALGGRVDVAAM+TISGVKATYLLAV+FL
Sbjct: 781  DELELNALHNPGSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFL 840

Query: 841  EIIRFSSNGGILNGGSNVNASRSAFCCVFEYLKTPNLLPAVSQCLTAIVHRAFETAVSWL 900
            EIIRF SNGGILNG S+V+ASRSAF CVFEYLKTPNL PAVSQCLTAIVHRAFETAVSWL
Sbjct: 841  EIIRFISNGGILNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWL 900

Query: 901  EDRISDTGNEAEVRDSTLFAHTCYLIKSMSQRDEHVRDIAVNLLTQLRDKFPQVMWNSSC 960
            EDRIS TG +A  R+ T +AH C+LIKSMSQRDEHVRDI+VNLLTQLRDKFPQV+W+SSC
Sbjct: 901  EDRISLTGKDARNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSC 960

Query: 961  LDSLLFSMHNDAPSTVVSDPAWIVTVRSLYQRVVREWIVKSLSYAPCTCQGLLQEKLCKA 1020
            LDSLLFS+H++ PSTVV+DPAW   VRSLYQ+VVREWI+ SLSYAPCT QGLLQ+KLCKA
Sbjct: 961  LDSLLFSVHDNTPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKA 1020

Query: 1021 NTWQRAQHTPDVISLLSEIRIGTSKNEHWTGIQTANIPAVITAAAAASGADLKLTEAFNL 1080
            NTWQRAQ T DV+SLLSEI+IGT KNE W+GI+TANIPAV+ AAAAASGA+LK++EAFNL
Sbjct: 1021 NTWQRAQTTTDVVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNL 1080

Query: 1081 EVLSTGMVSATVKCNHAGEIAGMRRLYNSIGGFQTGVAGSTGLGFGQGLQRLITGALPQQ 1140
            EVL TG+VSATVKCNHAGEIAGMRRLYNSIGGFQ   +GST  GFG GLQRLI+GA  Q 
Sbjct: 1081 EVLGTGVVSATVKCNHAGEIAGMRRLYNSIGGFQ---SGSTPSGFGGGLQRLISGAFSQA 1140

Query: 1141 PQNEDDSFNGILIMKFVQSLQQFVSGAEKGRGLDKLKFRETCSQATALLLSNLASESKTN 1200
            PQ EDDSFN +LI +FV+ LQQFV+ AEKG  ++K +FRETCSQATALLLSNL  ESKTN
Sbjct: 1141 PQPEDDSFNEMLIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTN 1200

Query: 1201 IEGFAQLIRLLCWCPAYISTPDAIETGVFIWTWLVSAAPELGSFVLAELVDAWLWTIDTK 1260
            +EGF+QL+RLLCWCPAYISTPDA+ETG+FIWTWLVSAAP+L S VLAELVDAW+WTIDTK
Sbjct: 1201 VEGFSQLLRLLCWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTK 1260

Query: 1261 RGLFASDA-----------SLISGEPEVQPEIDPVEQIIAHRIWLGFFIDRFEVVRHNSV 1320
            RGLFASD             L  GEPE  PE DPV+QI+AHR+WLGF IDRFEVVRHNS 
Sbjct: 1261 RGLFASDVRYSGPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSA 1320

Query: 1321 EQLLLFGRLLQGSTKPPWNFSRHPAATGSFFTLMLLGLKFCSCQAQGNLQNFKTGLELLE 1380
            EQLLL GR+LQ ST   W F+RHPAA G+FF+LMLLGLKFCSCQ QGN+Q F++GL+LLE
Sbjct: 1321 EQLLLLGRMLQRSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLE 1380

Query: 1381 DRIYRASLGWFAHEPEWYDVKHVNFAQSEAQSVSIFLHYLSSERGNSLQSDAKMRGRENG 1440
            DRIYR SLGWFAH+PEWYDV   NF  SEA SVS+F+H+LS+E   S QSD+K + RE+G
Sbjct: 1381 DRIYRTSLGWFAHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESG 1440

Query: 1441 ISLIDSNYQYHPVWGHLENYAVGREKRRQLLLMLCQHEADRLEVWAQP-NIKESTPSRPK 1500
             +LID   QYHPVWG ++NY +G+EKR+QLLLMLCQHEADRL+VWAQP + K+S  SR K
Sbjct: 1441 -NLIDVTDQYHPVWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLK 1500

Query: 1501 LTAEKWIEHARTAFSVDPRIAFSMVSRFPTNAFLRVEMNQLVQLHILDIRSIPEALPYFV 1560
            +++EKW E+A+TAFSVDPRIA S+ SRFP NA ++ E+ QLVQ +I+D+R+IPEALPYFV
Sbjct: 1501 ISSEKWTEYAKTAFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFV 1560

Query: 1561 TPKAVDENSELLRQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPEKVTFFM 1620
            TPK V+ENS LL+QLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPE+VTFFM
Sbjct: 1561 TPKNVEENSVLLQQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFM 1620

Query: 1621 PQLVQALRYDEGRLVEGYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLA 1680
            PQLVQ+LRYD+GRLVEGYLLRA QRSDIFAHILIWHLQGE  + ++ KD +  KN +F  
Sbjct: 1621 PQLVQSLRYDDGRLVEGYLLRATQRSDIFAHILIWHLQGE-DVQETPKDGSIDKNAAFQE 1680

Query: 1681 LLPVVRQHIIDGFTPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEME 1740
            +LP VRQHIIDGF+P ALD+F REFDFFDKVTSISGVLFPLPK+ERRAGIRRELEKIEM+
Sbjct: 1681 ILPQVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIEMQ 1740

Query: 1741 GEDLYLPTATNKLVRGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGD 1800
            G+DLYLPTA NKLVRGI+VDSGIPLQSAAKVPIM+TFNV+DRDGD +++KPQACIFKVGD
Sbjct: 1741 GDDLYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKVGD 1800

Query: 1801 DCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTD 1860
            DCRQDVLALQVISLLRDIFQA GLNLYLFPYGVLPTG  RGIIEVVPNTRSRSQMGETTD
Sbjct: 1801 DCRQDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGETTD 1860

Query: 1861 GGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVH 1920
            GGLYEIFQQDYGPVGS  FE ARENF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVH
Sbjct: 1861 GGLYEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVH 1920

Query: 1921 IDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRH 1980
            IDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR 
Sbjct: 1921 IDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRQ 1980

Query: 1981 MDGIINTVLLMLDSGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAG 1990
            MDGII+TV +ML+SGLPCFSRGDPIGNLRKRFHPEM++REAA+FMI VCTDAYNKWTTAG
Sbjct: 1981 MDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTTAG 2028

BLAST of PI0013678 vs. TAIR 10
Match: AT1G51040.1 (Protein kinase superfamily protein )

HSP 1 Score: 710.7 bits (1833), Expect = 3.3e-204
Identity = 351/420 (83.57%), Postives = 378/420 (90.00%), Query Frame = 0

Query: 1570 LVEGYLLRAAQRSDIFAHILIWHLQGETSLPDSGKDVNSGKNGSFLALLPVVRQHIIDGF 1629
            +VEGYLLRAAQRSDIFAH LIWHLQGE S+ +  KD    KN SF  +L  VRQHI+DGF
Sbjct: 115  MVEGYLLRAAQRSDIFAHTLIWHLQGE-SVEEIVKDGAFDKNASFQEILSDVRQHIVDGF 174

Query: 1630 TPKALDLFKREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMEGEDLYLPTATNKL 1689
            TPKAL+ F REFDFF+KVTSISG L PLPK+ER AGIRRELEKI+M+GEDLYLPTA NKL
Sbjct: 175  TPKALNFFSREFDFFEKVTSISGALLPLPKEERVAGIRRELEKIKMQGEDLYLPTAPNKL 234

Query: 1690 VRGIQVDSGIPLQSAAKVPIMVTFNVVDRDGDPNNIKPQACIFKVGDDCRQDVLALQVIS 1749
            VR IQVDSGIPLQSAAKVPIM+TFNVVD DGD N++KPQACIFKVGDDCRQDVLALQVIS
Sbjct: 235  VRVIQVDSGIPLQSAAKVPIMITFNVVDLDGDHNDVKPQACIFKVGDDCRQDVLALQVIS 294

Query: 1750 LLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRSRSQMGETTDGGLYEIFQQDYGP 1809
            LL DIFQAVGLNLYLFPYGVLPT        VVPNTRSRSQMGETTDGGLYEIFQQ+YG 
Sbjct: 295  LLGDIFQAVGLNLYLFPYGVLPT--------VVPNTRSRSQMGETTDGGLYEIFQQNYGL 354

Query: 1810 VGSPNFEAARENFIVSSAGYAVASLLLQPKDRHNGNLLFDNVGRLVHIDFGFILETSPGG 1869
            VGS  FE AR NF++SSAGYAVASLLLQPKDRHNGNLLFD+VGRLVHIDFGFILETSPGG
Sbjct: 355  VGSTTFETARANFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLVHIDFGFILETSPGG 414

Query: 1870 NMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFVSLCVKGYLAARRHMDGIINTVLLMLD 1929
            NMRFE+AHFKLSHEMTQLLDPSGVMKS+TW+ FVSLCVKGYLAARR+MD II+TV +ML+
Sbjct: 415  NMRFENAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARRYMDEIISTVQMMLE 474

Query: 1930 SGLPCFSRGDPIGNLRKRFHPEMNDREAANFMIRVCTDAYNKWTTAGYDLIQYLQQGIEK 1989
            SGLPCFSRGDPIGNLRKRFHPEM++REAA FMI VCTDAYNKWTTAGYDLIQYLQQG+EK
Sbjct: 475  SGLPCFSRGDPIGNLRKRFHPEMSEREAALFMINVCTDAYNKWTTAGYDLIQYLQQGVEK 525

HSP 2 Score: 110.9 bits (276), Expect = 1.2e-23
Identity = 54/117 (46.15%), Postives = 76/117 (64.96%), Query Frame = 0

Query: 1   MDALIELCDLIAQNPAFFSEKLSWICSRCPPSEAILAGSPAISRSQINAVLAVARLLSKC 60
           M+ L ELCD+I QNP  F +KL WIC +CP S+ + A SP  S+S +NA+LAV R+LSK 
Sbjct: 1   MERLNELCDIIEQNPKQFLDKLPWICQQCPQSKLLRAESPRFSQSHLNAILAVTRILSKI 60

Query: 61  PDSVGLRPKSAVLEFLRSIPLSFSLSFWPQSYGNDAIASFFNEFLNYTSKACELSTD 118
            D+     K  VL+FL+++P SF  SFWP S   ++I++F+  FL Y S   +L  +
Sbjct: 61  VDTTDENAKFVVLDFLQTVPKSFHRSFWPYSLSLESISAFYCSFLGYVSCLSQLMVE 117

BLAST of PI0013678 vs. TAIR 10
Match: AT5G64070.1 (phosphatidylinositol 4-OH kinase beta1 )

HSP 1 Score: 211.5 bits (537), Expect = 6.4e-54
Identity = 114/266 (42.86%), Postives = 163/266 (61.28%), Query Frame = 0

Query: 1728 QACIFKVGDDCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRS 1787
            ++ I K GDDCRQ+ LA+Q+IS   DIFQ  GL L+L PY VL T     +IE +P+T S
Sbjct: 859  RSIIVKSGDDCRQEHLAVQLISHFYDIFQEAGLPLWLRPYEVLVTSSYTALIETIPDTAS 918

Query: 1788 ----RSQMGETTDGGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHN 1847
                +S+    T   L + F   Y    SP+F+ A+ NF+ S AGY++   LLQ KDRHN
Sbjct: 919  IHSIKSRYPNIT--SLRDFFVAKY-KENSPSFKLAQRNFVESMAGYSLVCYLLQVKDRHN 978

Query: 1848 GNLLFDNVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLD-PSGVMKSETWNLF 1907
            GNLL D  G ++HIDFGF+L  SPGG + FESA FKL+ E+ +++D  +  + SE ++ F
Sbjct: 979  GNLLLDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDADGVPSEFFDYF 1038

Query: 1908 VSLCVKGYLAARRHMDGIINTVLLMLDSGLPCFSRGD-PIGNLRKRFHPEMNDREAANFM 1967
              LC++G+L  R+H + II  V ++ DSG PCF  G   I NLRKRFH  + + +  + +
Sbjct: 1039 KVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLV 1098

Query: 1968 IRVCTDAYNKWTTAGYDLIQYLQQGI 1988
            + + + + + W T  YD  Q +  GI
Sbjct: 1099 LSLISSSLDAWRTRQYDYYQRVLNGI 1120

BLAST of PI0013678 vs. TAIR 10
Match: AT5G09350.1 (phosphatidylinositol 4-OH kinase beta2 )

HSP 1 Score: 210.7 bits (535), Expect = 1.1e-53
Identity = 113/266 (42.48%), Postives = 162/266 (60.90%), Query Frame = 0

Query: 1728 QACIFKVGDDCRQDVLALQVISLLRDIFQAVGLNLYLFPYGVLPTGPGRGIIEVVPNTRS 1787
            ++ I K GDDCRQ+ LA+Q+IS   DIFQ  GL L+L PY VL T     +IE +P+T S
Sbjct: 854  RSIIVKSGDDCRQEHLAVQLISHFFDIFQEAGLPLWLRPYEVLVTSSYTALIETIPDTAS 913

Query: 1788 ----RSQMGETTDGGLYEIFQQDYGPVGSPNFEAARENFIVSSAGYAVASLLLQPKDRHN 1847
                +S+    T   L + F   +    SP+F+ A+ NF+ S AGY++   LLQ KDRHN
Sbjct: 914  IHSIKSRYPNIT--SLRDFFDAKF-KENSPSFKLAQRNFVESMAGYSLVCYLLQIKDRHN 973

Query: 1848 GNLLFDNVGRLVHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGV-MKSETWNLF 1907
            GNLL D  G ++HIDFGF+L  SPGG + FESA FKL+ E+ +++D     + SE ++ F
Sbjct: 974  GNLLMDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDAEGLPSEFFDYF 1033

Query: 1908 VSLCVKGYLAARRHMDGIINTVLLMLDSGLPCFSRGD-PIGNLRKRFHPEMNDREAANFM 1967
              LC++G+L  R+H + II  V ++ DSG PCF  G   I NLRKRFH  + + +  + +
Sbjct: 1034 KVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLV 1093

Query: 1968 IRVCTDAYNKWTTAGYDLIQYLQQGI 1988
            + + + + + W T  YD  Q +  GI
Sbjct: 1094 LSLISSSLDAWRTRQYDYYQRVLNGI 1115