PI0013644 (gene) Melon (PI 482460) v1

Overview
NamePI0013644
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr07: 814753 .. 817775 (-)
RNA-Seq ExpressionPI0013644
SyntenyPI0013644
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGATTTTCAATAAATTTGTGACAATTTTCTTCTTTTTCTGTTCTTGAGGTTTGCAACAGTCACGTCTCTTCTCTTCTCTATACAAAAATATTGTGAAATTTAGAGCAAAAATAACGTGAGCTTATGGACTATCTTCCAACATTAATTAAAAAAGGTAATTTGTTCTATACAATAGCGTTACATCCAAGATGACATTATGACATTAGCCTGACATAACAAAATCTTCCAGAGAAATCAAGAAGTGAACAGTACATACGATTTCAATTCCGAATTCGCTTCAAATTCCTTTTCAATTTTCTTTTTATTTAAACAAACCAACCACTTAACCAATGCCCAAACATCCCACAAATGGAAACCATCCACCACACCACCGTCCAAACCAACGGCATAAACCTCCACGTTGCCACCGCCGGCCCAGTAACCGGCCCACCCGTCCTCCTCCTCCACGGCTTCCCAGAGCTCTGGTACTCCTGGCGCCACCAGATCATTTTCCTCTCCTCCGCCGGCTACCGCGTTATCGCACCTGACCTCCGTGGGTACGGTGATTCCGACGCACCGCCGTCGTCCGAGACCTACACCGCCCTACACATCGTGGGGGATGTGGTGGGGTTACTCAATGAGCTAGGGATAGATAAGGTGTTATTAGTGGGGCATGATTGGGGTGCCTTAATTGCTTGGTACTTTTGTTTGTTTAGGCCAGATCGGATCAAAGCGTCCGTAATCTTGAGCGTCCAATTTTTCCCTAGAAATCCAAAAATACCATTTATTGAAGGCTTCAAGGCTGTTCTTGGGGACCAGTTTTATATGGTCAGATTTCAGGTCATTTTCTTACCCTGTCCTACAATTTTGTTTTGTGAATTGTAGTATGAATCAATTAAATTTTTAAGAAAATGTGGTTATTTTGAGTGATGTGTGTGTGTTGAAATGAAAATTGATAGGAACCGGGAAAGGCAGAGGAAGAGTTTGCATCGGTGGATATAAGAGAGTTTTTCAAAAACGTGTTATCAAATAGGGACCCACAGGCTCCATATTTGCCTGGCGAAGTGAAATTTGAAGGTGTCCCACCCCCGTCTCTAGCACCCTGGCTCACTCCCCAAGATATTAACTTTTATGCCCAGAAGTTCTCCCAGACTGGCTTCACCGGCGGTCTCAACTATTACCGAGCTTTTGACCGGTAACAACCCTTCTACATTATTTCCTTAATCAAAATATTTGGTTAAATTATAAATTTACACCTTATCATTTTTCTTTTAAAAATATTAGAATTTAATATTTTTAATATTTAAATTTTTATAAGTAAAAACCCTAATAAAACCTCATGAATACAACCATAAAGACTATATTTACGGTATCAAATTTTGAGACTAAATTTTATTAATAAAGTATACAAATTAAACTTTTAAGTTTTTTTACCACTTGCATAAATAGTATTTACCTCGAAAAAATAATAAAATATTTCGTACAACATAAAATAAATAAAAAAAAAATAGTAAGAGAATAAAAACGCAGCCACCATTTAAACACTTCTACTCAAAAATCTTAAATTAAAAATTATTAATGATAGTAACTATTAGTGATATATATGAATTGATAACTGTTAAAATAAATTGTTAAAAGTTATTATCATTGATAGATTGTATTATTGATGGTTCTTAATTTGATAAATGTGTTACAATTGTTTTTTATGGTCACTGTGAATGAATTTCCATCAATAACAATTACTAAAACACAAACCTGACCTTTAAAATTTTTAAATTATATTTTTTAAATTGTACTATTTGTTATAAGTTTTCCTTTTCAAAATCATAATAGCCAAATTTGGAAAAAAGAGTTAGATGTATTTACACGTTTCTTTGTGAGAAAAATTTATAGTATTATTGAAATCCCGTATATTCACATTCAGTCCTTAAATTATTTAATCAAAAAGATTGAATTTTTCTTTAATAGCTTTTAACTTTTTTAACAATTTAGAATTCATTTGGATGGAGCAGAAAAGTATTTTTATTTTTTTTAAAATGACTTGTTCTTTTATAATTAAACACTTTAAATATATAATCTCTTAGCTTAATTAGTTTTTGTAATTTTAAATTTGTAACAATTTTAACAGTTAAAATGATTATGAAATTATTTTAACAGGTTTTAGTTTCAATAATTGATTGTAAATTTGTTTATTAAAAGTGATTTCATGTCATATACATCCAAAAAATAATTGTAAATATAATAATATTTAGAACCAACTTCTAGGATCTATCAACGATAGTAATAGGAAGAACCTACCAGAAATATAGTTTATGGTCCATAAATTTTACTATATTTGTAATTATTTTAAAATGTTATTATCAATTTAATTATTAATTCCAGAAGTGTTGCATTGCACTTACATGTTATTAGGTCAATGTTAGTTATTCAAATGAAAGTATAATTCATTTTCTTTTAATTTTAATTTTATAACGTACACTTTACTGCTAACTCAATATTTTTAACTGATAGAGAAATTGTAAGAGTATTGTCTCGGACTGTGGAGATGATAAATGAATAAATGTGTTTTGAGTTTGAAATACAGGACTTGGGAGCTGACAGCGCCATGGACAGGGGCACAAATTAAGGTACCAGTAAAATTCATAGTGGGGGATTTGGATCTGACTTATCATTTTCCAGGAGCTCAAGACTACATCAATGGCGACGCCTTCAGAAAGGATGTCCCTGGTTTGGAAGAAGTGATCGTTGTGAAAGACGCTTCTCATTTCATCAACCAAGAAAGGCCTGATGAAATCAACTGCCATATTCATGATTTCTTCAACAAATTCTGCTGATTCTCATCTTTCTCTGTTTCCCTTTTCAACTTCTATATATGTTTTTATATATATAACCTAATAAGTTCTCATGTGGGATTGATTTGTGTGTGTCTCCCCTTTTGTTTTTCATTTGTGTATAGAATAATAAGGATTTTAATAAGCCAAAGTAAATATATACATATGTATGCTTCAGTTTGCTTTTTAATGATATTTTTCTTTCCCCTTTTT

mRNA sequence

TGATTTTCAATAAATTTGTGACAATTTTCTTCTTTTTCTGTTCTTGAGGTTTGCAACAGTCACGTCTCTTCTCTTCTCTATACAAAAATATTGTGAAATTTAGAGCAAAAATAACGTGAGCTTATGGACTATCTTCCAACATTAATTAAAAAAGGTAATTTGTTCTATACAATAGCGTTACATCCAAGATGACATTATGACATTAGCCTGACATAACAAAATCTTCCAGAGAAATCAAGAAGTGAACAGTACATACGATTTCAATTCCGAATTCGCTTCAAATTCCTTTTCAATTTTCTTTTTATTTAAACAAACCAACCACTTAACCAATGCCCAAACATCCCACAAATGGAAACCATCCACCACACCACCGTCCAAACCAACGGCATAAACCTCCACGTTGCCACCGCCGGCCCAGTAACCGGCCCACCCGTCCTCCTCCTCCACGGCTTCCCAGAGCTCTGGTACTCCTGGCGCCACCAGATCATTTTCCTCTCCTCCGCCGGCTACCGCGTTATCGCACCTGACCTCCGTGGGTACGGTGATTCCGACGCACCGCCGTCGTCCGAGACCTACACCGCCCTACACATCGTGGGGGATGTGGTGGGGTTACTCAATGAGCTAGGGATAGATAAGGTGTTATTAGTGGGGCATGATTGGGGTGCCTTAATTGCTTGGTACTTTTGTTTGTTTAGGCCAGATCGGATCAAAGCGTCCGTAATCTTGAGCGTCCAATTTTTCCCTAGAAATCCAAAAATACCATTTATTGAAGGCTTCAAGGCTGTTCTTGGGGACCAGTTTTATATGGTCAGATTTCAGGAACCGGGAAAGGCAGAGGAAGAGTTTGCATCGGTGGATATAAGAGAGTTTTTCAAAAACGTGTTATCAAATAGGGACCCACAGGCTCCATATTTGCCTGGCGAAGTGAAATTTGAAGGTGTCCCACCCCCGTCTCTAGCACCCTGGCTCACTCCCCAAGATATTAACTTTTATGCCCAGAAGTTCTCCCAGACTGGCTTCACCGGCGGTCTCAACTATTACCGAGCTTTTGACCGGACTTGGGAGCTGACAGCGCCATGGACAGGGGCACAAATTAAGGTACCAGTAAAATTCATAGTGGGGGATTTGGATCTGACTTATCATTTTCCAGGAGCTCAAGACTACATCAATGGCGACGCCTTCAGAAAGGATGTCCCTGGTTTGGAAGAAGTGATCGTTGTGAAAGACGCTTCTCATTTCATCAACCAAGAAAGGCCTGATGAAATCAACTGCCATATTCATGATTTCTTCAACAAATTCTGCTGATTCTCATCTTTCTCTGTTTCCCTTTTCAACTTCTATATATGTTTTTATATATATAACCTAATAAGTTCTCATGTGGGATTGATTTGTGTGTGTCTCCCCTTTTGTTTTTCATTTGTGTATAGAATAATAAGGATTTTAATAAGCCAAAGTAAATATATACATATGTATGCTTCAGTTTGCTTTTTAATGATATTTTTCTTTCCCCTTTTT

Coding sequence (CDS)

ATGGAAACCATCCACCACACCACCGTCCAAACCAACGGCATAAACCTCCACGTTGCCACCGCCGGCCCAGTAACCGGCCCACCCGTCCTCCTCCTCCACGGCTTCCCAGAGCTCTGGTACTCCTGGCGCCACCAGATCATTTTCCTCTCCTCCGCCGGCTACCGCGTTATCGCACCTGACCTCCGTGGGTACGGTGATTCCGACGCACCGCCGTCGTCCGAGACCTACACCGCCCTACACATCGTGGGGGATGTGGTGGGGTTACTCAATGAGCTAGGGATAGATAAGGTGTTATTAGTGGGGCATGATTGGGGTGCCTTAATTGCTTGGTACTTTTGTTTGTTTAGGCCAGATCGGATCAAAGCGTCCGTAATCTTGAGCGTCCAATTTTTCCCTAGAAATCCAAAAATACCATTTATTGAAGGCTTCAAGGCTGTTCTTGGGGACCAGTTTTATATGGTCAGATTTCAGGAACCGGGAAAGGCAGAGGAAGAGTTTGCATCGGTGGATATAAGAGAGTTTTTCAAAAACGTGTTATCAAATAGGGACCCACAGGCTCCATATTTGCCTGGCGAAGTGAAATTTGAAGGTGTCCCACCCCCGTCTCTAGCACCCTGGCTCACTCCCCAAGATATTAACTTTTATGCCCAGAAGTTCTCCCAGACTGGCTTCACCGGCGGTCTCAACTATTACCGAGCTTTTGACCGGACTTGGGAGCTGACAGCGCCATGGACAGGGGCACAAATTAAGGTACCAGTAAAATTCATAGTGGGGGATTTGGATCTGACTTATCATTTTCCAGGAGCTCAAGACTACATCAATGGCGACGCCTTCAGAAAGGATGTCCCTGGTTTGGAAGAAGTGATCGTTGTGAAAGACGCTTCTCATTTCATCAACCAAGAAAGGCCTGATGAAATCAACTGCCATATTCATGATTTCTTCAACAAATTCTGCTGA

Protein sequence

METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPDLRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRIKASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLSNRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQERPDEINCHIHDFFNKFC
Homology
BLAST of PI0013644 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.9e-58
Identity = 123/314 (39.17%), Postives = 173/314 (55.10%), Query Frame = 0

Query: 9   VQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPDLRGYGDSD 68
           V TNG+ L V  AG    P V+L HGFPEL YSWRHQI  L+ AGY V+APD RGYG S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  APPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRIKASVILSV 128
            P + E Y    +  D+VGLL+++G ++ + VGHDWGA++ W   L   DR+ A   LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 QFFPRNPKIPFIEGFKAVLGDQ-FYMVRFQEPGKAEEEFASVDIREFFKNVLSNRDP--- 188
              PR  ++P  + F++  G+  FY++ FQEPG A+ E      R   + +   R P   
Sbjct: 129 PALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQ 188

Query: 189 ---QAPYLPGEVKF-EGVPPPS-LAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWE 248
                   PG   F + +P P+ L  W++ ++++ Y  +F++TGFTGGLN+YR FDR WE
Sbjct: 189 SAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNWE 248

Query: 249 LTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFIN 308
            TA   G  I VP  FI G  D    F         D   + + G    +++  A H++ 
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTF------TRTDRAAEVISGPYREVLIDGAGHWLQ 308

Query: 309 QERPDEINCHIHDF 314
           QERP E+   + +F
Sbjct: 309 QERPGEVTAALLEF 315

BLAST of PI0013644 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.7e-51
Identity = 113/303 (37.29%), Postives = 166/303 (54.79%), Query Frame = 0

Query: 13  GINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPDLRGYGDSDAPPS 72
           G+ LH    G  +GP V L HGFPE W+SWR+QI  L+ AG+RV+A D++GYG+S APP 
Sbjct: 247 GVRLHFVEMG--SGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPE 306

Query: 73  SETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRIKASVILSVQFFP 132
            E Y+   +  D+V  LN+LG+ + + +GHDWG ++ W   LF P+R++A   L+  F P
Sbjct: 307 IEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMP 366

Query: 133 RNPKIPFIEGFKA--VLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLSNRDPQAPYLP 192
            NP +  +E  KA  V   Q Y   FQEPG AE E    ++   FKN     D +     
Sbjct: 367 SNPNVSPMEIIKANPVFDYQLY---FQEPGVAEAELEQ-NLDRTFKNFFRAHD-ETFLTT 426

Query: 193 GEVK-----FEGVP-PPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWELTAPW 252
             V+     F G P  PSL+  +T +DI FY Q+F ++GF G LN+YR  +R W+     
Sbjct: 427 NRVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKG 486

Query: 253 TGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQERPD 308
           +G +I +P   +  + DL  H   ++   N       +P L+    +KD  H+   ++P 
Sbjct: 487 SGRKILIPALMVTAENDLVLHPKMSKHMENW------IPHLKRG-HIKDCGHWTQIDKPA 535

BLAST of PI0013644 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 2.8e-49
Identity = 105/301 (34.88%), Postives = 160/301 (53.16%), Query Frame = 0

Query: 14  INLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPDLRGYGDSDAPPSS 73
           + LH    G  +GP V L HGFPE WYSWR+QI  L+ AGYRV+A D++GYG+S APP  
Sbjct: 248 VRLHFVELG--SGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEI 307

Query: 74  ETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRIKASVILSVQFFPR 133
           E Y    +  ++V  L++LG+ + + +GHDWG ++ WY  LF P+R++A   L+  F P 
Sbjct: 308 EEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPA 367

Query: 134 NPKIPFIEGFKA--VLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLSNRDPQAPYL-- 193
           NP +  +E  KA  V   Q Y   FQEPG AE E    ++   FK++    D     +  
Sbjct: 368 NPNMSPLESIKANPVFDYQLY---FQEPGVAEAELEQ-NLSRTFKSLFRASDESVLSMHK 427

Query: 194 ---PGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWELTAPWTG 253
               G +       PSL+  +T ++I FY Q+F ++GF G LN+YR  +R W+      G
Sbjct: 428 VCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLG 487

Query: 254 AQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQERPDEI 308
            +I +P   +  + D     P    ++        +P L+    ++D  H+   ++P E+
Sbjct: 488 RKILIPALMVTAEKDFVL-VPQMSQHM-----EDWIPHLKRG-HIEDCGHWTQMDKPTEV 535

BLAST of PI0013644 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.8e-46
Identity = 99/301 (32.89%), Postives = 157/301 (52.16%), Query Frame = 0

Query: 13  GINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPDLRGYGDSDAPPS 72
           GI LH    G  +GP + L HGFPE W+SWR+QI  L+ AG+RV+A D++GYGDS +PP 
Sbjct: 245 GIRLHFVEMG--SGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPE 304

Query: 73  SETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRIKASVILSVQFFP 132
            E Y    +  ++V  LN+LGI + + +GHDW  ++ W   LF P+R++A   L+    P
Sbjct: 305 IEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMP 364

Query: 133 RNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLSNRDPQAPYLPGE 192
            NP++  +E  +++     Y + FQEPG AE E    ++   FK+     D        +
Sbjct: 365 PNPEVSPMEVIRSIPVFN-YQLYFQEPGVAEAELEK-NMSRTFKSFFRTSDDMGLLTVNK 424

Query: 193 VK-----FEGVP-PPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWELTAPWTG 252
                    G P  P ++   T ++I +Y Q+F ++GF G LN+YR  +R W+ +    G
Sbjct: 425 ATEMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALG 484

Query: 253 AQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQERPDEI 308
            +I VP   +  + D+      +++  N   F K          ++D  H+   E+P E+
Sbjct: 485 RKILVPALMVTAEKDIVLRPEMSKNMENWIPFLKRGH-------IEDCGHWTQIEKPAEV 534

BLAST of PI0013644 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 2.4e-45
Identity = 99/302 (32.78%), Postives = 162/302 (53.64%), Query Frame = 0

Query: 13  GINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPDLRGYGDSDAPPS 72
           GI LH    G  +GP + L HGFPE W+SWR+QI  L+ AG+RV+A D++GYGDS +PP 
Sbjct: 245 GIRLHFVEMG--SGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPE 304

Query: 73  SETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRIKASVILSVQFFP 132
            E Y    +  ++V  L++LGI + + +GHDW  ++ W   LF P+R++A   L+  F P
Sbjct: 305 IEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMP 364

Query: 133 RNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLSNRDPQAPYLP-- 192
            +P +  ++  +++     Y + FQEPG AE E    ++   FK+     D +  ++   
Sbjct: 365 PDPDVSPMKVIRSIPVFN-YQLYFQEPGVAEAELEK-NMSRTFKSFFRASD-ETGFIAVH 424

Query: 193 -----GEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWELTAPWT 252
                G +       P+L+   T ++I FY Q+F +TGF G LN+YR  +R W+ +    
Sbjct: 425 KATEIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGL 484

Query: 253 GAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQERPDE 308
           G +I VP   +  + D+      +++        K +P L+    ++D  H+   E+P E
Sbjct: 485 GRKILVPALMVTAEKDIVLRPEMSKN------MEKWIPFLKRG-HIEDCGHWTQIEKPTE 534

BLAST of PI0013644 vs. ExPASy TrEMBL
Match: A0A0A0KV84 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001910 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.4e-181
Identity = 298/318 (93.71%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           METI+H TVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSS GYRVIAPD
Sbjct: 1   METINHITVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSVGYRVIAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDSDAPPSS+TYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI
Sbjct: 61  LRGYGDSDAPPSSDTYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KASVILSVQFFPRNPK+ F+EGFKAVLGDQFYMVRFQEPGKAE+EFASVDIREFFKNV+S
Sbjct: 121 KASVILSVQFFPRNPKVSFVEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGE KFEGVPPPSLAPWLTPQDI++YAQKFS +GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEEKFEGVPPPSLAPWLTPQDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPG QDYINGDAFRKDVPGLEEVIV+KD SHFINQ
Sbjct: 241 TAPWTAAEIKVPVKFIVGDLDLTYHFPGGQDYINGDAFRKDVPGLEEVIVMKDTSHFINQ 300

Query: 301 ERPDEINCHIHDFFNKFC 319
           ERPDEINCHIHDFFNKFC
Sbjct: 301 ERPDEINCHIHDFFNKFC 318

BLAST of PI0013644 vs. ExPASy TrEMBL
Match: A0A5A7TNE2 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001190 PE=4 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 3.8e-174
Identity = 289/318 (90.88%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           METI HT VQTNG+NLHVATAGPVTGPPVLLLHGFPELWYSWR QI+FLSSAGYRVIAPD
Sbjct: 33  METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 92

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDSDAPPSS++YTALHIVGDVVGLL+E GIDKVLLVGHDWGALIAWY CLFRPDRI
Sbjct: 93  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 152

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KASVILSVQFFPRNPK+ FIEGFKAVLGDQFYMVRFQEPGKAE+EFASVDIREFFKNV+S
Sbjct: 153 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 212

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGEVKFEGVPPPSLAPWLTP+DI++YAQKFS +GFTGGLNYYRAFDRTWEL
Sbjct: 213 NRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 272

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPGAQDYINGDAF KDVPGLEEVIV+ DASHFINQ
Sbjct: 273 TAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVIVIGDASHFINQ 332

Query: 301 ERPDEINCHIHDFFNKFC 319
           ERP EIN HIHDFF  FC
Sbjct: 333 ERPVEINSHIHDFFRNFC 350

BLAST of PI0013644 vs. ExPASy TrEMBL
Match: A0A1S3BXU7 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494759 PE=4 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 3.8e-174
Identity = 289/318 (90.88%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           METI HT VQTNG+NLHVATAGPVTGPPVLLLHGFPELWYSWR QI+FLSSAGYRVIAPD
Sbjct: 1   METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDSDAPPSS++YTALHIVGDVVGLL+E GIDKVLLVGHDWGALIAWY CLFRPDRI
Sbjct: 61  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KASVILSVQFFPRNPK+ FIEGFKAVLGDQFYMVRFQEPGKAE+EFASVDIREFFKNV+S
Sbjct: 121 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGEVKFEGVPPPSLAPWLTP+DI++YAQKFS +GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPGAQDYINGDAF KDVPGLEEVIV+ DASHFINQ
Sbjct: 241 TAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVIVIGDASHFINQ 300

Query: 301 ERPDEINCHIHDFFNKFC 319
           ERP EIN HIHDFF  FC
Sbjct: 301 ERPVEINSHIHDFFRNFC 318

BLAST of PI0013644 vs. ExPASy TrEMBL
Match: A0A6J1DP12 (uncharacterized protein LOC111022455 OS=Momordica charantia OX=3673 GN=LOC111022455 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 4.5e-159
Identity = 259/318 (81.45%), Postives = 292/318 (91.82%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           METI HTTV+TNG+ +HVA  G  +GPPVLLLHGFPELWYSWRHQ+++L+SAGYR IAPD
Sbjct: 1   METIEHTTVKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDS+APPS+  YTALHIVGD++G+L++LGI+KVLLVGHDWGALIAWYFCLFRPDRI
Sbjct: 61  LRGYGDSEAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KA V LSVQFFPR+P  PFI+GFKA LGDQ YMVRFQEPGKAE EFA+VDIREFFKNVLS
Sbjct: 121 KALVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLS 180

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP+APYLP EV FEGVPPPSLAPWLTP+DI+ YA KF+QTGFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TAPWT A+I+VPVKFIVGDLDLTYHFPGA++YING AF+ DVPGLEEV+V++DASHFINQ
Sbjct: 241 TAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQ 300

Query: 301 ERPDEINCHIHDFFNKFC 319
           E+P EIN HIH+FF KFC
Sbjct: 301 EKPHEINSHIHNFFKKFC 316

BLAST of PI0013644 vs. ExPASy TrEMBL
Match: A0A6J1KD67 (uncharacterized protein LOC111492796 OS=Cucurbita maxima OX=3661 GN=LOC111492796 PE=4 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 2.9e-158
Identity = 256/318 (80.50%), Postives = 289/318 (90.88%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           ME IHHTTV+TNGINLH+A  G  TGPPVLLLHGFPE WYSWRHQ+I+LSS GYR IAPD
Sbjct: 1   MEAIHHTTVKTNGINLHIAAVG--TGPPVLLLHGFPEFWYSWRHQLIYLSSIGYRAIAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDSDAPPS++ YT LHIVGD+VGLL+ELGI+KVLLVG+DWGA+IAWY CLFRPDRI
Sbjct: 61  LRGYGDSDAPPSADAYTTLHIVGDLVGLLDELGIEKVLLVGNDWGAIIAWYLCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KA+VILSVQFFPRNP  PF+E FK+VLGDQFYMVRFQ+PG+AEEEF +VDI++FF  V+S
Sbjct: 121 KAAVILSVQFFPRNPTTPFVEAFKSVLGDQFYMVRFQQPGQAEEEFGTVDIKKFFNEVMS 180

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP+APYLPGEVKFEG+PPP  A WLTP+DI+ YA  FS+TGFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPRAPYLPGEVKFEGLPPPPPATWLTPEDIDVYADMFSRTGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TA WT A+IKVPVKFIVGDLDLTYHFPGAQ+YI+GD F++DVPGLEEV+V+KD SHFINQ
Sbjct: 241 TAAWTRAEIKVPVKFIVGDLDLTYHFPGAQEYIHGDGFKRDVPGLEEVVVMKDTSHFINQ 300

Query: 301 ERPDEINCHIHDFFNKFC 319
           ERP+E N HIHDFFNKFC
Sbjct: 301 ERPNETNAHIHDFFNKFC 316

BLAST of PI0013644 vs. NCBI nr
Match: XP_004152243.1 (uncharacterized protein LOC101204575 [Cucumis sativus] >KGN52824.1 hypothetical protein Csa_014427 [Cucumis sativus])

HSP 1 Score: 644.4 bits (1661), Expect = 5.0e-181
Identity = 298/318 (93.71%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           METI+H TVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSS GYRVIAPD
Sbjct: 1   METINHITVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSVGYRVIAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDSDAPPSS+TYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI
Sbjct: 61  LRGYGDSDAPPSSDTYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KASVILSVQFFPRNPK+ F+EGFKAVLGDQFYMVRFQEPGKAE+EFASVDIREFFKNV+S
Sbjct: 121 KASVILSVQFFPRNPKVSFVEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGE KFEGVPPPSLAPWLTPQDI++YAQKFS +GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEEKFEGVPPPSLAPWLTPQDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPG QDYINGDAFRKDVPGLEEVIV+KD SHFINQ
Sbjct: 241 TAPWTAAEIKVPVKFIVGDLDLTYHFPGGQDYINGDAFRKDVPGLEEVIVMKDTSHFINQ 300

Query: 301 ERPDEINCHIHDFFNKFC 319
           ERPDEINCHIHDFFNKFC
Sbjct: 301 ERPDEINCHIHDFFNKFC 318

BLAST of PI0013644 vs. NCBI nr
Match: XP_038905173.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 629.8 bits (1623), Expect = 1.3e-176
Identity = 290/318 (91.19%), Postives = 307/318 (96.54%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           M+TIHHTTVQTNGI LHVA AGPVTGPPVLLLHGFPELWYSWRHQI+FLSSAGYRVIAPD
Sbjct: 1   MDTIHHTTVQTNGIKLHVAAAGPVTGPPVLLLHGFPELWYSWRHQIVFLSSAGYRVIAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           L GYGDSDAP SS++YTALHIVGD+VGLL+ELGI+KVLLVGHDWGALIAWYFCLFRPD+I
Sbjct: 61  LHGYGDSDAPSSSDSYTALHIVGDLVGLLDELGIEKVLLVGHDWGALIAWYFCLFRPDKI 120

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KASVILSVQFFPRNPK PFIEGFKAVLGDQFYMVRFQEPGKAEEEFA+VDIREFFKN LS
Sbjct: 121 KASVILSVQFFPRNPKTPFIEGFKAVLGDQFYMVRFQEPGKAEEEFATVDIREFFKNTLS 180

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP+APYLPGEVKFEGVPPP LAPWLTP+DI+FYAQKFS TGFTG LNYYRAFDRTWEL
Sbjct: 181 NRDPRAPYLPGEVKFEGVPPPPLAPWLTPEDIDFYAQKFSHTGFTGSLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TAPWTGAQI+VPVKFIVGDLDLTYHFPGAQ YI+GDAF+KDVPGL+EVIV+KDASHFINQ
Sbjct: 241 TAPWTGAQIEVPVKFIVGDLDLTYHFPGAQKYIHGDAFKKDVPGLDEVIVMKDASHFINQ 300

Query: 301 ERPDEINCHIHDFFNKFC 319
           ERP EINCHIHDFFNKFC
Sbjct: 301 ERPHEINCHIHDFFNKFC 318

BLAST of PI0013644 vs. NCBI nr
Match: KAA0044388.1 (bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK29516.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 620.5 bits (1599), Expect = 7.8e-174
Identity = 289/318 (90.88%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           METI HT VQTNG+NLHVATAGPVTGPPVLLLHGFPELWYSWR QI+FLSSAGYRVIAPD
Sbjct: 33  METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 92

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDSDAPPSS++YTALHIVGDVVGLL+E GIDKVLLVGHDWGALIAWY CLFRPDRI
Sbjct: 93  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 152

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KASVILSVQFFPRNPK+ FIEGFKAVLGDQFYMVRFQEPGKAE+EFASVDIREFFKNV+S
Sbjct: 153 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 212

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGEVKFEGVPPPSLAPWLTP+DI++YAQKFS +GFTGGLNYYRAFDRTWEL
Sbjct: 213 NRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 272

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPGAQDYINGDAF KDVPGLEEVIV+ DASHFINQ
Sbjct: 273 TAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVIVIGDASHFINQ 332

Query: 301 ERPDEINCHIHDFFNKFC 319
           ERP EIN HIHDFF  FC
Sbjct: 333 ERPVEINSHIHDFFRNFC 350

BLAST of PI0013644 vs. NCBI nr
Match: XP_008454324.2 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 620.5 bits (1599), Expect = 7.8e-174
Identity = 289/318 (90.88%), Postives = 303/318 (95.28%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           METI HT VQTNG+NLHVATAGPVTGPPVLLLHGFPELWYSWR QI+FLSSAGYRVIAPD
Sbjct: 1   METIQHTNVQTNGVNLHVATAGPVTGPPVLLLHGFPELWYSWRSQILFLSSAGYRVIAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDSDAPPSS++YTALHIVGDVVGLL+E GIDKVLLVGHDWGALIAWY CLFRPDRI
Sbjct: 61  LRGYGDSDAPPSSDSYTALHIVGDVVGLLDEFGIDKVLLVGHDWGALIAWYCCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KASVILSVQFFPRNPK+ FIEGFKAVLGDQFYMVRFQEPGKAE+EFASVDIREFFKNV+S
Sbjct: 121 KASVILSVQFFPRNPKMSFIEGFKAVLGDQFYMVRFQEPGKAEKEFASVDIREFFKNVMS 180

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP APYLPGEVKFEGVPPPSLAPWLTP+DI++YAQKFS +GFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPSAPYLPGEVKFEGVPPPSLAPWLTPKDIDYYAQKFSHSGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TAPWT A+IKVPVKFIVGDLDLTYHFPGAQDYINGDAF KDVPGLEEVIV+ DASHFINQ
Sbjct: 241 TAPWTKAEIKVPVKFIVGDLDLTYHFPGAQDYINGDAFEKDVPGLEEVIVIGDASHFINQ 300

Query: 301 ERPDEINCHIHDFFNKFC 319
           ERP EIN HIHDFF  FC
Sbjct: 301 ERPVEINSHIHDFFRNFC 318

BLAST of PI0013644 vs. NCBI nr
Match: XP_022155322.1 (uncharacterized protein LOC111022455 [Momordica charantia])

HSP 1 Score: 570.5 bits (1469), Expect = 9.3e-159
Identity = 259/318 (81.45%), Postives = 292/318 (91.82%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           METI HTTV+TNG+ +HVA  G  +GPPVLLLHGFPELWYSWRHQ+++L+SAGYR IAPD
Sbjct: 1   METIEHTTVKTNGVKIHVAAVG--SGPPVLLLHGFPELWYSWRHQMVYLASAGYRAIAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDS+APPS+  YTALHIVGD++G+L++LGI+KVLLVGHDWGALIAWYFCLFRPDRI
Sbjct: 61  LRGYGDSEAPPSAGAYTALHIVGDLIGVLDDLGIEKVLLVGHDWGALIAWYFCLFRPDRI 120

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
           KA V LSVQFFPR+P  PFI+GFKA LGDQ YMVRFQEPGKAE EFA+VDIREFFKNVLS
Sbjct: 121 KALVNLSVQFFPRDPTTPFIQGFKAALGDQLYMVRFQEPGKAEAEFATVDIREFFKNVLS 180

Query: 181 NRDPQAPYLPGEVKFEGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWEL 240
           NRDP+APYLP EV FEGVPPPSLAPWLTP+DI+ YA KF+QTGFTGGLNYYRAFDRTWEL
Sbjct: 181 NRDPRAPYLPNEVLFEGVPPPSLAPWLTPEDIDVYAAKFAQTGFTGGLNYYRAFDRTWEL 240

Query: 241 TAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFINQ 300
           TAPWT A+I+VPVKFIVGDLDLTYHFPGA++YING AF+ DVPGLEEV+V++DASHFINQ
Sbjct: 241 TAPWTAAKIRVPVKFIVGDLDLTYHFPGAREYINGAAFKNDVPGLEEVVVIEDASHFINQ 300

Query: 301 ERPDEINCHIHDFFNKFC 319
           E+P EIN HIH+FF KFC
Sbjct: 301 EKPHEINSHIHNFFKKFC 316

BLAST of PI0013644 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 434.1 bits (1115), Expect = 9.6e-122
Identity = 190/318 (59.75%), Postives = 246/318 (77.36%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           ME I HTT+ TNGIN+HVA+ G  +GP +L +HGFP+LWYSWRHQ++  ++ GYR IAPD
Sbjct: 1   MEKIEHTTISTNGINMHVASIG--SGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNELGIDKVLLVGHDWGALIAWYFCLFRPDRI 120
           LRGYGDSDAPPS E+YT LHIVGD+VGLL+ LG+D+V LVGHDWGA++AW+ C+ RPDR+
Sbjct: 61  LRGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRV 120

Query: 121 KASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVLS 180
            A V  SV F PRNP +  ++ F+A+ GD +Y+ RFQEPG+ EE+FA VD ++      +
Sbjct: 121 NALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFT 180

Query: 181 NRDPQAPYLPGEVKFEGVP-PPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAFDRTWE 240
           +R+P+ P +P  V F G+P PPSL  WLT QD+ FY  KFSQ GFTGGLNYYRA + +WE
Sbjct: 181 SRNPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWE 240

Query: 241 LTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDASHFIN 300
           LTAPWTG QIKVPVKFIVGDLD+TY+ PG ++YI+    +K VP L+EV+V++   HF++
Sbjct: 241 LTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLH 300

Query: 301 QERPDEINCHIHDFFNKF 318
           QE+PDE+  HI+ FF KF
Sbjct: 301 QEKPDEVTDHIYGFFKKF 316

BLAST of PI0013644 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 394.8 bits (1013), Expect = 6.5e-110
Identity = 180/323 (55.73%), Postives = 228/323 (70.59%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPD 60
           ME I H  V  NGI +H+A  GP  GP VLLLHGFP+LWY+WRHQI  LSS GYR +APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDSDAPPSSETYTALHIVGDVVGLLNEL--GIDKVLLVGHDWGALIAWYFCLFRPD 120
           LRGYGDSD+P S   YT L++VGD+V LL+ +    +KV LVGHDWGA+I W+ CLFRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RIKASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNV 180
           +I   V LSV +  RNPK+  ++GFKAV GD +Y+ RFQEPGK E E AS D R F +N+
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LSNRDPQAPYLPGEVKFEGVPPPS-----LAPWLTPQDINFYAQKFSQTGFTGGLNYYRA 240
            + R    P LP +  F   P P+     L  W + +D++FY  KF + GFTGGLNYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 FDRTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKD 300
            D  WELTAPWTGA+I+VPVKF+ GD D+ Y  PG ++YI+G  F  DVP L+E++V++D
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 ASHFINQERPDEINCHIHDFFNK 317
           A HF+NQE+P E+  HI+DFF K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of PI0013644 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 353.2 bits (905), Expect = 2.2e-97
Identity = 166/324 (51.23%), Postives = 225/324 (69.44%), Query Frame = 0

Query: 1   METIHHTTVQTNGINLHVAT---AGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVI 60
           ++ + H T++ NGIN+HVA    +G    P +L LHGFPELWY+WRHQ++ LSS GYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDSDAPPSSETYTALHIVGDVVGLLNEL-GIDK-VLLVGHDWGALIAWYFCLF 120
           APDLRGYGD++AP   E YT L++ GDVV L++ + G DK V +VGHDWGA+IAW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRIKASVILSVQFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFF 180
           RP+++KA V +SV F PRNP    +   + V GD +Y+ RFQ+ G+ E EF  +      
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KNVLSNRDPQAPYLPGEVKFEGV--PPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRA 240
           K  L+ + P    LP +  F+       +L  WLT +D+++Y  K+   GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 FDRTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKD 300
            DR WELTAPWTGA+I+VPVKFI+GD DLTY+FPGA++YING  F++DVP L+E +V+K 
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 ASHFINQERPDEINCHIHDFFNKF 318
             HF+++E PD IN HIH+FF+KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of PI0013644 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 342.8 bits (878), Expect = 2.9e-94
Identity = 164/324 (50.62%), Postives = 219/324 (67.59%), Query Frame = 0

Query: 4   IHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPDLRG 63
           + H  V+ NGI++HVA  GP  GP VLLLHGFPELWYSWRHQI  L++ GYR +APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDSDAPPSSETYTALHIVGDVVGLLNELGI---DKVLLVGHDWGALIAWYFCLFRPDRI 123
           YGDSDAP    +YT  +IVGD++ +++ L     +KV +VGHDWGALIAWY CLFRPDR+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KASVILSV--QFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNV 183
           KA V LSV   F P +P +  ++  +A  GD +Y+ RFQE G  E E A V      K +
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LSNRDPQAPYLPGEVKF-----EGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRA 243
           L+ R P    +P +  F     E +P PS   WLT +D+ ++  KF + GF+G +NYYR 
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIPLPS---WLTEEDVAYFVSKFEEKGFSGPVNYYRN 240

Query: 244 FDRTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKD 303
           F+R  EL  PW G++I+VP KF++G+LDL Y+ PG ++YI+G  F++DVP LEE +V++ 
Sbjct: 241 FNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEG 300

Query: 304 ASHFINQERPDEINCHIHDFFNKF 318
            +HFINQE+P EI   I DF +KF
Sbjct: 301 VAHFINQEKPQEILQIILDFISKF 321

BLAST of PI0013644 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.4e-91
Identity = 159/323 (49.23%), Postives = 218/323 (67.49%), Query Frame = 0

Query: 4   IHHTTVQTNGINLHVATAGPVTGPPVLLLHGFPELWYSWRHQIIFLSSAGYRVIAPDLRG 63
           + H  V+ NGI++HVA  GP  G  VLLLHGFPELWYSWRHQI  L++ GYR +APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGDSDAPPSSETYTALHIVGDVVGLLNELGID--KVLLVGHDWGALIAWYFCLFRPDRIK 123
           YGDSDAP    ++T  +IVGD+V +++ L  +  KV +VGHDWGALIAWY CLFRPD++K
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ASVILSV--QFFPRNPKIPFIEGFKAVLGDQFYMVRFQEPGKAEEEFASVDIREFFKNVL 183
           A V LSV   F+P +P +  ++  +AV G+ +Y+ RFQE G  E E A V      K +L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 SNRDPQAPYLPGEVKF-----EGVPPPSLAPWLTPQDINFYAQKFSQTGFTGGLNYYRAF 243
           + R P    +P +  F     E +P PS   WLT +D+ ++  KF + GF G +NYYR F
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIPLPS---WLTEEDVAYFVSKFKEKGFCGPVNYYRNF 240

Query: 244 DRTWELTAPWTGAQIKVPVKFIVGDLDLTYHFPGAQDYINGDAFRKDVPGLEEVIVVKDA 303
           +R  EL  PW G++I+VP KF++G+LDL Y+ PG ++YI+G  F++DVP +EE +V++  
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGV 300

Query: 304 SHFINQERPDEINCHIHDFFNKF 318
           +HF+NQE+P EI   I DF + F
Sbjct: 301 AHFLNQEKPQEILQIILDFISTF 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I6YGS01.9e-5839.17Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C21.7e-5137.29Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P349132.8e-4934.88Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P802993.8e-4632.89Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
P349142.4e-4532.78Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KV842.4e-18193.71AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001... [more]
A0A5A7TNE23.8e-17490.88Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BXU73.8e-17490.88bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494759 PE... [more]
A0A6J1DP124.5e-15981.45uncharacterized protein LOC111022455 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1KD672.9e-15880.50uncharacterized protein LOC111492796 OS=Cucurbita maxima OX=3661 GN=LOC111492796... [more]
Match NameE-valueIdentityDescription
XP_004152243.15.0e-18193.71uncharacterized protein LOC101204575 [Cucumis sativus] >KGN52824.1 hypothetical ... [more]
XP_038905173.11.3e-17691.19epoxide hydrolase A-like [Benincasa hispida][more]
KAA0044388.17.8e-17490.88bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa] >TYK29516.1 bif... [more]
XP_008454324.27.8e-17490.88PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo][more]
XP_022155322.19.3e-15981.45uncharacterized protein LOC111022455 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G02340.19.6e-12259.75alpha/beta-Hydrolases superfamily protein [more]
AT3G05600.16.5e-11055.73alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.12.2e-9751.23alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.12.9e-9450.62soluble epoxide hydrolase [more]
AT2G26750.11.4e-9149.23alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 99..112
score: 33.25
coord: 113..126
score: 22.17
coord: 53..68
score: 53.23
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 27..140
e-value: 1.5E-24
score: 87.1
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 33..51
score: 57.66
coord: 53..68
score: 64.77
coord: 113..126
score: 42.86
coord: 291..313
score: 36.17
coord: 99..112
score: 55.52
coord: 251..267
score: 38.77
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..317
e-value: 4.5E-109
score: 367.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..316
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..317
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0013644.1PI0013644.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity