PI0012625 (gene) Melon (PI 482460) v1

Overview
NamePI0012625
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr03: 15594294 .. 15596166 (+)
RNA-Seq ExpressionPI0012625
SyntenyPI0012625
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGAACTATTCAAGTCATTCCCCTTCTAGCCCTAGTCTTAGTCTTGTTTTCATCTCTTTCAGCTTATTCCTTGCTCTTGTCAACACGAGGAAGATGGATCATTGACTCGCAAACAGGACAACGAGTGAAACTCGTGTGCGTGAATTGGCCTTCCCATACACAAAGCATGTTGATAGAAGGTCTCAACCATAGGCCTTTAAAAGAACTAGCAGAAGAAGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATATGCAACCCACATGTTCACTCGCTACGCAAATAGGACAGTTGAAGAAAACTTCGATCTTCTTGATTTAAAACAAGCAAAAGTTGGATTGGCTCAATATAATCCTTTTGTGTTGAACAAGACTATTGTTGAAGCTTATGAAGCTGTTGTTGATGTGTTGGGGGCAACTGGTCTGATGGTGATTGCTGACAATCACATGAGCCAACCAAGATGGTGTTGCTCTCTTGACGATGGGAATGGCTTCTTTGGAAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCCACGGTATGTAACATCAAAAGATTTTCTTATCAATTTCTTGAAACTATACTAAAGCATTTAAAAACTTCTAGAATGTTTGCATTAAACAAGGAATATTTTTTTGTGATTAGGTGGTGGGGATGAGTCTACGAAATGAGATACGAGGAATGATGGAAAATGCAAATGATTGGAACAATTACGTAACTCAAGGAGTAACCATAATCCATAACATAAATCCAGCAGTTTTAGTAATTGTTTCAGGCTTAAATTATGACAATGATCTCCGATGCTTAAAGGAGAAGCCTTTGAATGTAAGCACCTTAGACAATAAGTTGGTTTTCGAGGTACACCTGTATTCTTTCAGCGGAGATTCTGAGAGCAAGTTTGTGCAACAACCATTGAACAATATTTGTGCAAAAATTATGAATAACTTTATAGATCATGCTGAGTTTGTAATTGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCAGAAAATCGCTTCATGAGTTGTTTCACAGCTCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGCCAAGCAGAGCTTGCAGAAACTTTTGGAGTGCTTGAATCTAATTGGACTCAAATTAAGAACCCTAATTTTGTCCAGAAGTTTCAACTATTGCAGACCATGTTGCAAGGTAACTATATTAAAGATGTTCAATTTATGCAGGGACCTATAATTAACTAGTAAACTACACTATGATTTGGAAAAGTATGTTTAATAGTTCAACTAGTAGTTTTGTAAAAAAGTAACAATTTGGATAAAAAAAATTTTTATGGTACGCAGATCCAAATTCCAATGCATCGTTCTCATATGTTATATATCATCCACAAAGTGGGCAATGTATCGAAGTCTCTAATGACAACAAAGAAATTTTCCTCACCAATTGCTCCACCTCAAGTCGATGGAATCATGACAATGATAGTACTCCAATAAGGATGTCAAGCACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGCCTGGAGGCATCTCTTTCAACTGACTGCTTAGGCAAACAAAGTGTTTGGAGTGCTATTTCAAACTCTAAGCTTCATTTGGCAATTGTCACAGAAGATGGAAAGAGTCTTTGTCTACAGATTGAAAGCTCAAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACAAACGATGATCCAACTTGCCTCCAAGACACACAAAGCCAATGGTTTGAACTTGTTGGAACCAACACATTGTGA

mRNA sequence

ATGGAAAGAACTATTCAAGTCATTCCCCTTCTAGCCCTAGTCTTAGTCTTGTTTTCATCTCTTTCAGCTTATTCCTTGCTCTTGTCAACACGAGGAAGATGGATCATTGACTCGCAAACAGGACAACGAGTGAAACTCGTGTGCGTGAATTGGCCTTCCCATACACAAAGCATGTTGATAGAAGGTCTCAACCATAGGCCTTTAAAAGAACTAGCAGAAGAAGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATATGCAACCCACATGTTCACTCGCTACGCAAATAGGACAGTTGAAGAAAACTTCGATCTTCTTGATTTAAAACAAGCAAAAGTTGGATTGGCTCAATATAATCCTTTTGTGTTGAACAAGACTATTGTTGAAGCTTATGAAGCTGTTGTTGATGTGTTGGGGGCAACTGGTCTGATGGTGATTGCTGACAATCACATGAGCCAACCAAGATGGTGTTGCTCTCTTGACGATGGGAATGGCTTCTTTGGAAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCCACGGTGGTGGGGATGAGTCTACGAAATGAGATACGAGGAATGATGGAAAATGCAAATGATTGGAACAATTACGTAACTCAAGGAGTAACCATAATCCATAACATAAATCCAGCAGTTTTAGTAATTGTTTCAGGCTTAAATTATGACAATGATCTCCGATGCTTAAAGGAGAAGCCTTTGAATGTAAGCACCTTAGACAATAAGTTGGTTTTCGAGGTACACCTGTATTCTTTCAGCGGAGATTCTGAGAGCAAGTTTGTGCAACAACCATTGAACAATATTTGTGCAAAAATTATGAATAACTTTATAGATCATGCTGAGTTTGTAATTGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCAGAAAATCGCTTCATGAGTTGTTTCACAGCTCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGCCAAGCAGAGCTTGCAGAAACTTTTGGAGTGCTTGAATCTAATTGGACTCAAATTAAGAACCCTAATTTTGTCCAGAAGTTTCAACTATTGCAGACCATGTTGCAAGATCCAAATTCCAATGCATCGTTCTCATATGTTATATATCATCCACAAAGTGGGCAATGTATCGAAGTCTCTAATGACAACAAAGAAATTTTCCTCACCAATTGCTCCACCTCAAGTCGATGGAATCATGACAATGATAGTACTCCAATAAGGATGTCAAGCACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGCCTGGAGGCATCTCTTTCAACTGACTGCTTAGGCAAACAAAGTGTTTGGAGTGCTATTTCAAACTCTAAGCTTCATTTGGCAATTGTCACAGAAGATGGAAAGAGTCTTTGTCTACAGATTGAAAGCTCAAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACAAACGATGATCCAACTTGCCTCCAAGACACACAAAGCCAATGGTTTGAACTTGTTGGAACCAACACATTGTGA

Coding sequence (CDS)

ATGGAAAGAACTATTCAAGTCATTCCCCTTCTAGCCCTAGTCTTAGTCTTGTTTTCATCTCTTTCAGCTTATTCCTTGCTCTTGTCAACACGAGGAAGATGGATCATTGACTCGCAAACAGGACAACGAGTGAAACTCGTGTGCGTGAATTGGCCTTCCCATACACAAAGCATGTTGATAGAAGGTCTCAACCATAGGCCTTTAAAAGAACTAGCAGAAGAAGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATATGCAACCCACATGTTCACTCGCTACGCAAATAGGACAGTTGAAGAAAACTTCGATCTTCTTGATTTAAAACAAGCAAAAGTTGGATTGGCTCAATATAATCCTTTTGTGTTGAACAAGACTATTGTTGAAGCTTATGAAGCTGTTGTTGATGTGTTGGGGGCAACTGGTCTGATGGTGATTGCTGACAATCACATGAGCCAACCAAGATGGTGTTGCTCTCTTGACGATGGGAATGGCTTCTTTGGAAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCCACGGTGGTGGGGATGAGTCTACGAAATGAGATACGAGGAATGATGGAAAATGCAAATGATTGGAACAATTACGTAACTCAAGGAGTAACCATAATCCATAACATAAATCCAGCAGTTTTAGTAATTGTTTCAGGCTTAAATTATGACAATGATCTCCGATGCTTAAAGGAGAAGCCTTTGAATGTAAGCACCTTAGACAATAAGTTGGTTTTCGAGGTACACCTGTATTCTTTCAGCGGAGATTCTGAGAGCAAGTTTGTGCAACAACCATTGAACAATATTTGTGCAAAAATTATGAATAACTTTATAGATCATGCTGAGTTTGTAATTGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCAGAAAATCGCTTCATGAGTTGTTTCACAGCTCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGCCAAGCAGAGCTTGCAGAAACTTTTGGAGTGCTTGAATCTAATTGGACTCAAATTAAGAACCCTAATTTTGTCCAGAAGTTTCAACTATTGCAGACCATGTTGCAAGATCCAAATTCCAATGCATCGTTCTCATATGTTATATATCATCCACAAAGTGGGCAATGTATCGAAGTCTCTAATGACAACAAAGAAATTTTCCTCACCAATTGCTCCACCTCAAGTCGATGGAATCATGACAATGATAGTACTCCAATAAGGATGTCAAGCACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGCCTGGAGGCATCTCTTTCAACTGACTGCTTAGGCAAACAAAGTGTTTGGAGTGCTATTTCAAACTCTAAGCTTCATTTGGCAATTGTCACAGAAGATGGAAAGAGTCTTTGTCTACAGATTGAAAGCTCAAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACAAACGATGATCCAACTTGCCTCCAAGACACACAAAGCCAATGGTTTGAACTTGTTGGAACCAACACATTGTGA

Protein sequence

MERTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEGLNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYNPFVLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNKEIFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISNSKLHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL
Homology
BLAST of PI0012625 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.3e-115
Identity = 225/541 (41.59%), Postives = 323/541 (59.70%), Query Frame = 0

Query: 10  LLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEGLNHRPLK 69
           LL  +L+L  +  ++SL L TRGRWI+D  TG RVKL CVNW  H +  L EGLN  P+ 
Sbjct: 13  LLTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVA 72

Query: 70  ELAEEAIKLRFNCVRLTYATHMFTR--YANRTVEENFDLLDLKQAKVGLAQYNPFVLNKT 129
            +A     L FNCVRLTY+ HM TR  Y N TV + F  L+L +A  G+   NP +L+  
Sbjct: 73  TVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLG 132

Query: 130 IVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQ 189
            V AY  VV  L   G+MVI DNH+S+P+WCC++DDGNGFFG+RYF+P  W++GL L+A 
Sbjct: 133 HVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMAT 192

Query: 190 RFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYDNDLRC 249
            FNN   VV MSLRNE+RG       W+ ++  G   +H  NP VLVI+SGL +D DL  
Sbjct: 193 YFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSF 252

Query: 250 LKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFVIEGSN 309
           L   P+ +     K+V+E H YSF     +       N++C      F  +  FV   +N
Sbjct: 253 LPVLPVTL-PFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNVGFVTSSAN 312

Query: 310 --PFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ---AE 369
               PLF+SE+G DQR V+D +NR+++C  A+LA++DLDWALWT  GSYYYR  +    +
Sbjct: 313 ATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKD 372

Query: 370 LAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPN-SNASFSYVIYHPQSGQCIEVSNDN 429
             ET+G    +W++I+NP+F+ + + +Q  +QDP  +   +  +IYHP SG C+E S   
Sbjct: 373 FEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVE-SGIG 432

Query: 430 KEIFLTNC-STSSRWNHD-NDSTPIRMSSTGLCLKASGEGLEASLSTDCLG-KQSVWSAI 489
             + L +C S  SRWN+D +   PI +  +  C+   G GL A ++ +C     ++WS +
Sbjct: 433 NTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTV 492

Query: 490 SNSKLHLA--IVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQ-DTQSQWFELVGT 537
           S+++L L   ++ +DGK   + ++ S S  I TN CIC  D     + + + QWF+++ T
Sbjct: 493 SSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICITDSHCYPKLNPEKQWFKVITT 547

BLAST of PI0012625 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 1.2e-12
Identity = 98/417 (23.50%), Postives = 164/417 (39.33%), Query Frame = 0

Query: 3   RTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVN-WPSHTQSMLIE 62
           RT   + L   + +      +YS+      R I+D  +G+ V+L  VN +   T + ++ 
Sbjct: 5   RTASTLALATALALAAGPAFSYSI---NNSRQIVD-DSGKVVQLKGVNVFGFETGNHVMH 64

Query: 63  GLNHRPLKELAEEAIKLRFNCVRLTYATHMF---TRYANRTVEENFDLLDLKQAKVGLAQ 122
           GL  R  K++  +   L FN VRL +        T  A+     N DL  L   +     
Sbjct: 65  GLWARNWKDMIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSRNADLQGLTSLQ----- 124

Query: 123 YNPFVLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEW 182
               +L+K I E          A G+ V+ D+H      C  + +   ++   Y + Q W
Sbjct: 125 ----ILDKVIAE--------FNARGMYVLLDHHTPD---CAGISE--LWYTGSYTEAQ-W 184

Query: 183 LQGLSLVAQRFNNKSTVVGMSLRNEIRGMM-----ENANDWNNYVTQGVTIIHNINPAVL 242
           L  L  VA R+ N   V+G+ L+NE  G         A DWN    +G   +  + P  L
Sbjct: 185 LADLRFVANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWL 244

Query: 243 VIVSGLN------------YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQ 302
           + V G+             +  +L+ L   PLN+    N+L+   H+Y         FVQ
Sbjct: 245 IAVEGITDNPVCSTNGGIFWGGNLQPLACTPLNIPA--NRLLLAPHVY-----GPDVFVQ 304

Query: 303 QPLNNICAKIMNNFIDHAEFVIEG-----SNPFPLFVSEYGYDQREVDDAENRFMSCFTA 362
              N+      +NF ++   + E      +    L + E+G    E D  +  +      
Sbjct: 305 SYFND------SNFPNNMPAIWERHFGQFAGTHALLLGEFGGKYGEGDARDKTWQDALVK 364

Query: 363 HLAQKDLDWAL-WTWQGSYYYREGQAELAETFGVLESNWTQIKNPNFVQKFQLLQTM 393
           +L  K ++    W+W              +T G+L  +WT ++      K  LL+T+
Sbjct: 365 YLRSKGINQGFYWSW---------NPNSGDTGGILRDDWTSVRQ----DKMTLLRTL 368

BLAST of PI0012625 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 6.0e-12
Identity = 86/409 (21.03%), Postives = 165/409 (40.34%), Query Frame = 0

Query: 1   MERTIQVIPLLALVLVLFS----SLSAYSL--LLSTRGRWIIDSQTGQRVKLVCVNW-PS 60
           +++T  VI  L +   L+     S  A S+     T+G  I+D ++G+      +NW   
Sbjct: 6   LKKTFFVIASLVMGFTLYGYTPVSADAASVKGYYHTQGNKIVD-ESGKEAAFNGLNWFGL 65

Query: 61  HTQSMLIEGLNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAK 120
            T +  + GL  R + ++ ++  K  +N +RL Y+  +F   +        D +D  +  
Sbjct: 66  ETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYSNQLFDSSSRP------DSIDYHK-- 125

Query: 121 VGLAQYNPFVLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYF 180
                 NP ++    ++  + +++  G  G+ +I D H            G+G     ++
Sbjct: 126 ------NPDLVGLNPIQIMDKLIEKAGQRGIQIILDRHR----------PGSGGQSELWY 185

Query: 181 DPQ----EWLQGLSLVAQRFNNKSTVVGMSLRNEIRGMME----NAN-DWNNYVTQGVTI 240
             Q     W+    ++A R+ N  TV+G  L NE  G       NA+ DW     +    
Sbjct: 186 TSQYPESRWISDWKMLADRYKNNPTVIGADLHNEPHGQASWGTGNASTDWRLAAQRAGNA 245

Query: 241 IHNINPAVLVIVSGLNYD-----------NDLRCLKEKPLNVSTLDNKLVFEVHLYSFSG 300
           I ++NP  L++V G++++            +L  +   P+ V  + N++V+  H Y   G
Sbjct: 246 ILSVNPNWLILVEGVDHNVQGNNSQYWWGGNLTGVANYPV-VLDVPNRVVYSPHDYG-PG 305

Query: 301 DSESKFVQQPL--NNICAKIMNNFIDHAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFM 360
            S   +   P   +N+ A       D     I   N  P+ V E+G    ++   E ++ 
Sbjct: 306 VSSQPWFNDPAFPSNLPA-----IWDQTWGYISKQNIAPVLVGEFGGRNVDLSCPEGKWQ 365

Query: 361 SCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLESNWTQIKNP 381
           +    ++   +L +  W+               +T G+L  +WT    P
Sbjct: 366 NALVHYIGANNLYFTYWSL---------NPNSGDTGGLLLDDWTTWNRP 373

BLAST of PI0012625 vs. ExPASy TrEMBL
Match: A0A0A0K853 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.3e-295
Identity = 504/539 (93.51%), Postives = 518/539 (96.10%), Query Frame = 0

Query: 1   MERTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLI 60
           MERTIQVI LLALV V FSS SAYSL LST GRWIIDSQ+G+RVKLVCVNWPSHTQSMLI
Sbjct: 1   MERTIQVILLLALVSV-FSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLI 60

Query: 61  EGLNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYN 120
           EGLNHRPLKELA+EAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL+QAK GLAQYN
Sbjct: 61  EGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYN 120

Query: 121 PFVLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ 180
           PFVLNKTI EAYEAVVDVLGA+GLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ
Sbjct: 121 PFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ 180

Query: 181 GLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLN 240
           GLSLVAQRFNNKSTVVGMSLRNE+RGMMENANDWNNYVTQGVT IH INPAVLVIVSGLN
Sbjct: 181 GLSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLN 240

Query: 241 YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAE 300
           YDNDLRCLK+KPLNVSTLDNKL FEVHLYSFSGDSESKFVQQPLNNICAKIM+ FIDHAE
Sbjct: 241 YDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAE 300

Query: 301 FVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 360
           FVIEG NPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ
Sbjct: 301 FVIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 360

Query: 361 AELAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSND 420
           AELAETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQCIEVSND
Sbjct: 361 AELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSND 420

Query: 421 NKEIFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISN 480
           NKEIFLTNCSTSSRW+HDNDSTPI+MSSTGLCLKASGEGLEASLSTDC+GKQS+WSAISN
Sbjct: 421 NKEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISN 480

Query: 481 SKLHLAIVTEDGKSLCLQ-IESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           S LHL  VTEDGKSLCLQ IESSNSSKIVTNSCICT +DPTCLQDTQSQWFELV TNTL
Sbjct: 481 SNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

BLAST of PI0012625 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 9.0e-277
Identity = 466/536 (86.94%), Postives = 498/536 (92.91%), Query Frame = 0

Query: 3   RTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEG 62
           RT   I L+ALV V FS LS  SL LSTRGRWIIDSQTG+RVKLVC+NWPSHTQSMLIEG
Sbjct: 2   RTFLTILLMALVFV-FSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEG 61

Query: 63  LNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYNPF 122
           LNHRPLK+LA+EAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDLKQAK GLAQYNPF
Sbjct: 62  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 121

Query: 123 VLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGL 182
           VLNKTI EAYEAVVDVLGA+GLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGL
Sbjct: 122 VLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 181

Query: 183 SLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYD 242
           SLVAQRF NKSTVVGMSLRNEIRG MENANDWN Y+TQGVT IHNIN  VLVIVSGLNYD
Sbjct: 182 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 241

Query: 243 NDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFV 302
           NDLRCLKEKPLNV TLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICA IMN FIDHA FV
Sbjct: 242 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFV 301

Query: 303 IEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAE 362
           ++GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE
Sbjct: 302 MQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAE 361

Query: 363 LAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNK 422
             E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSN SFSYVIYHPQS QCI+VSNDNK
Sbjct: 362 PGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNK 421

Query: 423 EIFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISNSK 482
           EIFLT+CST +RW+H ND+TPI+MSSTGLCLKASGEGLEASLSTDCL +QSVWSAISNSK
Sbjct: 422 EIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSK 481

Query: 483 LHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           LHLA VT+ GKSLCLQI+SSNSSK+VTNSCICTN DP CLQDT+SQWFELVGTNTL
Sbjct: 482 LHLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of PI0012625 vs. ExPASy TrEMBL
Match: A0A5A7TMX0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002070 PE=3 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 8.4e-275
Identity = 465/537 (86.59%), Postives = 498/537 (92.74%), Query Frame = 0

Query: 3   RTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEG 62
           RT  VI LLALV V FS L A SL LSTRGRWIIDSQTG+RVKLVC+NWPSHTQSMLIEG
Sbjct: 5   RTFLVILLLALVFV-FSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEG 64

Query: 63  LNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYNPF 122
           LNHRPLK+LA+EAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDLKQAK GLA YNPF
Sbjct: 65  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYNPF 124

Query: 123 VLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGL 182
           VLNKTI EAYEAVVD+LGA+GLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGL
Sbjct: 125 VLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 184

Query: 183 SLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYD 242
           SLVAQRF NKSTVVGMSLRNEIRG MENANDWN Y+TQGVT IHNIN  VLVIVSGLNYD
Sbjct: 185 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 244

Query: 243 NDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFV 302
           NDLRCLKEKPLNV TLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICA IMN FIDHA FV
Sbjct: 245 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFV 304

Query: 303 IEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAE 362
           ++GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE
Sbjct: 305 MQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAE 364

Query: 363 LAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNK 422
             E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSNASFSYVIYHPQS QCI+VSNDNK
Sbjct: 365 PGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSNDNK 424

Query: 423 EIFLTNC-STSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISNS 482
           EIFLT+C ST +RW+H ND+TPI+MSSTGLCLKASGEGLEASLSTDCL +QSVWSAISNS
Sbjct: 425 EIFLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNS 484

Query: 483 KLHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           KLHLA +T+ GKSLCLQI+SSN SK+VTNSCICTN DP CLQDT+SQWFELVGTNTL
Sbjct: 485 KLHLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 540

BLAST of PI0012625 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.1e-274
Identity = 463/536 (86.38%), Postives = 495/536 (92.35%), Query Frame = 0

Query: 3   RTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEG 62
           RT   I LLALV V FS LS  SL LSTRGRWIID QTG+RVKLVC+NWPSHTQSMLIEG
Sbjct: 2   RTFLTILLLALVFV-FSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEG 61

Query: 63  LNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYNPF 122
           LNHRPLK+LA+EAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDLKQAK GLAQYNPF
Sbjct: 62  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 121

Query: 123 VLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGL 182
           VLNKTI EAYEAVVD+LGA+GLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGL
Sbjct: 122 VLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 181

Query: 183 SLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYD 242
           SLVAQRF NKSTVVGMSLRNEIRG MENANDWN Y+TQGVT IHNIN  VLVIVSGLNYD
Sbjct: 182 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 241

Query: 243 NDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFV 302
           NDLRCLKEKPLNV TLDNKLVFEVHLYSFSGDSESKFV+QPLNNICA IMN FIDHA FV
Sbjct: 242 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFV 301

Query: 303 IEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAE 362
           ++GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE
Sbjct: 302 MQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAE 361

Query: 363 LAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNK 422
             E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSNASFSYVIYHPQ+ QCI+VSNDNK
Sbjct: 362 PGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNK 421

Query: 423 EIFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISNSK 482
           EIFLT+CST +RW+H ND+TPI+MSSTGLCLKASGEGLEASLSTDCL +QSVWSAISNSK
Sbjct: 422 EIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSK 481

Query: 483 LHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           LHLA V + G SLCLQI+SSNSSK+VTNSCICTN DP CLQDT+SQWFELV TNTL
Sbjct: 482 LHLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of PI0012625 vs. ExPASy TrEMBL
Match: A0A6J1JZU8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=3 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.4e-274
Identity = 466/538 (86.62%), Postives = 491/538 (91.26%), Query Frame = 0

Query: 1   MERTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLI 60
           M R  QV+  LA  L +F S   YSL LST+ +WIIDS TG+RVKLVCVNWPSHTQSMLI
Sbjct: 1   MGRIFQVVFFLA--LFVFLSPYVYSLPLSTKEKWIIDSTTGRRVKLVCVNWPSHTQSMLI 60

Query: 61  EGLNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYN 120
           EGLNHRPLKELA+EAIKLRFNCVRLTYAT MFTRYANRTVEENFDLLDL+QAK GLAQ N
Sbjct: 61  EGLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQNN 120

Query: 121 PFVLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ 180
           PFVLNKTI EAYEAVVDVLG +GLMVIADNH+SQPRWCCSLDDGNGFFG+RYFDPQEWLQ
Sbjct: 121 PFVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQ 180

Query: 181 GLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLN 240
           GLSLVAQRF+ KSTVVGMSLRNEIRG  ENANDWNNYVTQGVT IHNINP VLVIVSGLN
Sbjct: 181 GLSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLN 240

Query: 241 YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAE 300
           YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGD ESKF+ QPLNNICA I+N F+DHAE
Sbjct: 241 YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDPESKFINQPLNNICANIINGFVDHAE 300

Query: 301 FVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 360
           FV EGSNPFPLFVSEYGYDQREV+DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ
Sbjct: 301 FVTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 360

Query: 361 AELAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSND 420
           AE  ETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCI+VSND
Sbjct: 361 AEPGETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSND 420

Query: 421 NKEIFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISN 480
           NK+IF+ NCS SSRW HDNDSTPIRMSS GLCLK  GEGL  SLSTDCLG QS WSAISN
Sbjct: 421 NKDIFMGNCSISSRWTHDNDSTPIRMSSMGLCLKTIGEGLTPSLSTDCLGPQSSWSAISN 480

Query: 481 SKLHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           +KLHLA +++DGKSLCLQ+ESSNSSKIVTNSCICTN  P CLQDT+SQWFELV TNTL
Sbjct: 481 TKLHLATISQDGKSLCLQVESSNSSKIVTNSCICTNGAPNCLQDTRSQWFELVKTNTL 536

BLAST of PI0012625 vs. NCBI nr
Match: KAE8646606.1 (hypothetical protein Csa_005432 [Cucumis sativus])

HSP 1 Score: 1023.5 bits (2645), Expect = 6.8e-295
Identity = 504/539 (93.51%), Postives = 518/539 (96.10%), Query Frame = 0

Query: 1   MERTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLI 60
           MERTIQVI LLALV V FSS SAYSL LST GRWIIDSQ+G+RVKLVCVNWPSHTQSMLI
Sbjct: 29  MERTIQVILLLALVSV-FSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLI 88

Query: 61  EGLNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYN 120
           EGLNHRPLKELA+EAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL+QAK GLAQYN
Sbjct: 89  EGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYN 148

Query: 121 PFVLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ 180
           PFVLNKTI EAYEAVVDVLGA+GLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ
Sbjct: 149 PFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ 208

Query: 181 GLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLN 240
           GLSLVAQRFNNKSTVVGMSLRNE+RGMMENANDWNNYVTQGVT IH INPAVLVIVSGLN
Sbjct: 209 GLSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLN 268

Query: 241 YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAE 300
           YDNDLRCLK+KPLNVSTLDNKL FEVHLYSFSGDSESKFVQQPLNNICAKIM+ FIDHAE
Sbjct: 269 YDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAE 328

Query: 301 FVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 360
           FVIEG NPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ
Sbjct: 329 FVIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 388

Query: 361 AELAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSND 420
           AELAETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQCIEVSND
Sbjct: 389 AELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSND 448

Query: 421 NKEIFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISN 480
           NKEIFLTNCSTSSRW+HDNDSTPI+MSSTGLCLKASGEGLEASLSTDC+GKQS+WSAISN
Sbjct: 449 NKEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISN 508

Query: 481 SKLHLAIVTEDGKSLCLQ-IESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           S LHL  VTEDGKSLCLQ IESSNSSKIVTNSCICT +DPTCLQDTQSQWFELV TNTL
Sbjct: 509 SNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 566

BLAST of PI0012625 vs. NCBI nr
Match: XP_011658389.1 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 1023.5 bits (2645), Expect = 6.8e-295
Identity = 504/539 (93.51%), Postives = 518/539 (96.10%), Query Frame = 0

Query: 1   MERTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLI 60
           MERTIQVI LLALV V FSS SAYSL LST GRWIIDSQ+G+RVKLVCVNWPSHTQSMLI
Sbjct: 1   MERTIQVILLLALVSV-FSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLI 60

Query: 61  EGLNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYN 120
           EGLNHRPLKELA+EAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL+QAK GLAQYN
Sbjct: 61  EGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYN 120

Query: 121 PFVLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ 180
           PFVLNKTI EAYEAVVDVLGA+GLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ
Sbjct: 121 PFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ 180

Query: 181 GLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLN 240
           GLSLVAQRFNNKSTVVGMSLRNE+RGMMENANDWNNYVTQGVT IH INPAVLVIVSGLN
Sbjct: 181 GLSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLN 240

Query: 241 YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAE 300
           YDNDLRCLK+KPLNVSTLDNKL FEVHLYSFSGDSESKFVQQPLNNICAKIM+ FIDHAE
Sbjct: 241 YDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAE 300

Query: 301 FVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 360
           FVIEG NPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ
Sbjct: 301 FVIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 360

Query: 361 AELAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSND 420
           AELAETFGVL+SNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQCIEVSND
Sbjct: 361 AELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSND 420

Query: 421 NKEIFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISN 480
           NKEIFLTNCSTSSRW+HDNDSTPI+MSSTGLCLKASGEGLEASLSTDC+GKQS+WSAISN
Sbjct: 421 NKEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISN 480

Query: 481 SKLHLAIVTEDGKSLCLQ-IESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           S LHL  VTEDGKSLCLQ IESSNSSKIVTNSCICT +DPTCLQDTQSQWFELV TNTL
Sbjct: 481 SNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 538

BLAST of PI0012625 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 962.2 bits (2486), Expect = 1.8e-276
Identity = 466/536 (86.94%), Postives = 498/536 (92.91%), Query Frame = 0

Query: 3   RTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEG 62
           RT   I L+ALV V FS LS  SL LSTRGRWIIDSQTG+RVKLVC+NWPSHTQSMLIEG
Sbjct: 2   RTFLTILLMALVFV-FSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEG 61

Query: 63  LNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYNPF 122
           LNHRPLK+LA+EAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDLKQAK GLAQYNPF
Sbjct: 62  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPF 121

Query: 123 VLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGL 182
           VLNKTI EAYEAVVDVLGA+GLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGL
Sbjct: 122 VLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 181

Query: 183 SLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYD 242
           SLVAQRF NKSTVVGMSLRNEIRG MENANDWN Y+TQGVT IHNIN  VLVIVSGLNYD
Sbjct: 182 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 241

Query: 243 NDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFV 302
           NDLRCLKEKPLNV TLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICA IMN FIDHA FV
Sbjct: 242 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFV 301

Query: 303 IEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAE 362
           ++GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE
Sbjct: 302 MQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAE 361

Query: 363 LAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNK 422
             E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSN SFSYVIYHPQS QCI+VSNDNK
Sbjct: 362 PGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNK 421

Query: 423 EIFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISNSK 482
           EIFLT+CST +RW+H ND+TPI+MSSTGLCLKASGEGLEASLSTDCL +QSVWSAISNSK
Sbjct: 422 EIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSK 481

Query: 483 LHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           LHLA VT+ GKSLCLQI+SSNSSK+VTNSCICTN DP CLQDT+SQWFELVGTNTL
Sbjct: 482 LHLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of PI0012625 vs. NCBI nr
Match: XP_023533776.1 (uncharacterized protein LOC111795530 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 958.4 bits (2476), Expect = 2.7e-275
Identity = 470/538 (87.36%), Postives = 492/538 (91.45%), Query Frame = 0

Query: 1   MERTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLI 60
           M RT QV+  L L L +F S S YSL LST+GRWIIDS TG+RVKLVCVNWPSHTQSMLI
Sbjct: 1   MGRTFQVV--LFLALFVFLSPSVYSLPLSTKGRWIIDSTTGRRVKLVCVNWPSHTQSMLI 60

Query: 61  EGLNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYN 120
           EGLNHRPLKELA+EAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDL+ AK GLAQ N
Sbjct: 61  EGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEPAKAGLAQNN 120

Query: 121 PFVLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQ 180
           PFVLNKTI EAYEAVVDVLG +GLMVIADNH+SQPRWCCSLDDGNGFFG+RYFDPQEWLQ
Sbjct: 121 PFVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQ 180

Query: 181 GLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLN 240
           GLSLVAQRF+ KSTVVGMSLRNEIRG  ENANDWNNYVTQGVT IHNINP VLVIVSGLN
Sbjct: 181 GLSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLN 240

Query: 241 YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAE 300
           YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKF+ QPLNNICA I+N F+DHAE
Sbjct: 241 YDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFINQPLNNICANIINGFVDHAE 300

Query: 301 FVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 360
           FV EGSNPFPLFVSEYGYDQREV+DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ
Sbjct: 301 FVTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 360

Query: 361 AELAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSND 420
           AE  E FGVL+SNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCI+VSND
Sbjct: 361 AEPGEAFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSND 420

Query: 421 NKEIFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISN 480
           NK+IFL NCS SSRW HDNDSTPIRMSSTGLCLK SGEGL  SLSTDCLG Q+ WSAISN
Sbjct: 421 NKDIFLGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCLGPQNSWSAISN 480

Query: 481 SKLHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           +KLHLA +  D KSLCLQIESSNSSKIVTNS ICTN  P CL+DT+SQWFELV TNTL
Sbjct: 481 TKLHLATIAPDEKSLCLQIESSNSSKIVTNSFICTNGAPNCLEDTRSQWFELVKTNTL 536

BLAST of PI0012625 vs. NCBI nr
Match: KAA0042709.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 955.7 bits (2469), Expect = 1.7e-274
Identity = 465/537 (86.59%), Postives = 498/537 (92.74%), Query Frame = 0

Query: 3   RTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEG 62
           RT  VI LLALV V FS L A SL LSTRGRWIIDSQTG+RVKLVC+NWPSHTQSMLIEG
Sbjct: 5   RTFLVILLLALVFV-FSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEG 64

Query: 63  LNHRPLKELAEEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLKQAKVGLAQYNPF 122
           LNHRPLK+LA+EAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDLKQAK GLA YNPF
Sbjct: 65  LNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYNPF 124

Query: 123 VLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGL 182
           VLNKTI EAYEAVVD+LGA+GLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGL
Sbjct: 125 VLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGL 184

Query: 183 SLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYD 242
           SLVAQRF NKSTVVGMSLRNEIRG MENANDWN Y+TQGVT IHNIN  VLVIVSGLNYD
Sbjct: 185 SLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYD 244

Query: 243 NDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFV 302
           NDLRCLKEKPLNV TLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICA IMN FIDHA FV
Sbjct: 245 NDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFV 304

Query: 303 IEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAE 362
           ++GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE
Sbjct: 305 MQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAE 364

Query: 363 LAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVSNDNK 422
             E+FGVL+SNWTQ+KNPNF +KFQLLQTMLQDPNSNASFSYVIYHPQS QCI+VSNDNK
Sbjct: 365 PGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSNDNK 424

Query: 423 EIFLTNC-STSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQSVWSAISNS 482
           EIFLT+C ST +RW+H ND+TPI+MSSTGLCLKASGEGLEASLSTDCL +QSVWSAISNS
Sbjct: 425 EIFLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNS 484

Query: 483 KLHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELVGTNTL 539
           KLHLA +T+ GKSLCLQI+SSN SK+VTNSCICTN DP CLQDT+SQWFELVGTNTL
Sbjct: 485 KLHLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 540

BLAST of PI0012625 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 417.2 bits (1071), Expect = 2.1e-116
Identity = 210/511 (41.10%), Postives = 321/511 (62.82%), Query Frame = 0

Query: 28  LSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEGLNHRPLKELAEEAIKLRFNCVRLTY 87
           LST  RWI+D + G RVKLVC NWPSH Q ++ EGL+ +P+  +A++ +++ FNCVRLT+
Sbjct: 35  LSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVRLTW 94

Query: 88  ATHMFTRYA---NRTVEENFDLLDLKQAKVGLAQYNPFVLNKTIVEAYEAVVDVLGATGL 147
              + T      N TV ++F  L L    VG    NP +++  ++EAY+ VV  LG   +
Sbjct: 95  PLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGNNDV 154

Query: 148 MVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEI 207
           MVI DNH+++P WCC+ DDGNGFFG+++FDP  W+  L  +A  FN  S VVGMSLRNE+
Sbjct: 155 MVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMSLRNEL 214

Query: 208 RGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYDNDLRCLKEKPLNVSTLDNKLVF 267
           RG  +N NDW  Y+ QG   +H+ N  VLVI+SGL++D DL  ++ +P+ +S    KLVF
Sbjct: 215 RGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKLS-FTGKLVF 274

Query: 268 EVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFVIEGSNPFPLFVSEYGYDQREVD 327
           E+H YSFS D  S     P N+IC +++N   +   +++  +  FPLF+SE+G D+R V+
Sbjct: 275 ELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGYLL--NQGFPLFLSEFGIDERGVN 334

Query: 328 DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLESNWTQIKNPNFVQ 387
             +NR+  C T   A+ D+DW+LW   GSYY R+G+  + E +GVL+S+W  ++N +F+Q
Sbjct: 335 TNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSSFLQ 394

Query: 388 KFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEVS-NDNKEIFLTNCSTSSRWNHDNDSTP 447
           K   LQ+ LQ P        +++HP +G CI  S +D K + L  C++S  W++   +  
Sbjct: 395 KISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTKKA-- 454

Query: 448 IRMSSTGLCLKASGEGLEASLS-TDCLGKQSVWSAISNSKLHLAIVTEDGKSLCLQIESS 507
           +R+    LCL+++G     +++ T C    S W  IS S++HLA  T +  SLCL ++++
Sbjct: 455 LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLDVDTA 514

Query: 508 NSSKIVTNSCICTNDDPTCLQDTQSQWFELV 534
           N+  +V N+C C + D +C  +  SQWF+++
Sbjct: 515 NN--VVANACKCLSKDKSC--EPMSQWFKII 533

BLAST of PI0012625 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 380.2 bits (975), Expect = 2.8e-105
Identity = 215/531 (40.49%), Postives = 310/531 (58.38%), Query Frame = 0

Query: 14  VLVLFSSLSAYSLLLSTRGRWII-DSQTGQRVKLVCVNWPSHTQSMLIEGLNHRPLKELA 73
           V +L   ++ ++   ST  RWI+ D   G+RVKL CVNWPSH ++ + EGL+ +PL  +A
Sbjct: 9   VFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDAIA 68

Query: 74  EEAIKLRFNCVRLTYATHMFTR---YANRTVEENFDLLDLKQAKVGLAQYNPFVLNKTIV 133
           E+ + + FNCVRLT+  ++ T     A  TV ++     L +A  G   +NP +L+  ++
Sbjct: 69  EKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLPLI 128

Query: 134 EAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRF 193
           +A++ VV  L    +MVI DNH+SQP WCCS +DGNGFFG+++ +PQ W++GL  +A  F
Sbjct: 129 KAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMASMF 188

Query: 194 NN-KSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYDNDLRCL 253
            N  S VVGMSLRNE+RG  +N  DW  Y+ +G   +H++NP VLVIVSGLNY  DL  L
Sbjct: 189 ANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDLSFL 248

Query: 254 KEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFVIEGSNP 313
           +E+P  VS    K+VFE+H Y F    E       LN IC K     +  + F++E    
Sbjct: 249 RERPFEVS-FRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMKMSGFLLE--KG 308

Query: 314 FPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFG 373
            PLFVSE+G DQR  +  +N+F+SCF A  A +DLDW+LWT  GSYY RE      E++G
Sbjct: 309 IPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDESYG 368

Query: 374 VLESNWTQIKNPNFVQKFQLLQT-----MLQDPNSNASFSYVIYHPQSGQCIEVSNDNKE 433
           VL+ NW+ I+N   +Q    +QT     M   P        +++HP +G CI V     +
Sbjct: 369 VLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK------IMFHPSTGLCI-VRKSLFQ 428

Query: 434 IFLTNCSTSSRWNHDNDSTPIRMSSTGLCLKASGEGLEASLSTDCLGKQ-SVWSAISNSK 493
           + L +C+ S  W   +           LCLKA  +G    L         S W   S+SK
Sbjct: 429 LKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFSDSK 488

Query: 494 LHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELV 534
           + L+ +T++G S+CL +++ N++ IVTNSC C   + +C  D +SQWF+LV
Sbjct: 489 MQLSSITKNGFSVCLDVDTENNN-IVTNSCKCLRGNSSC--DPRSQWFKLV 522

BLAST of PI0012625 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 377.9 bits (969), Expect = 1.4e-104
Identity = 198/513 (38.60%), Postives = 310/513 (60.43%), Query Frame = 0

Query: 28  LSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEGLNHRPLKELAEEAIKLRFNCVRLTY 87
           LST  RWIID + GQRVKL CVNWPSH Q ++ EGL+ + + +LA++ + + FNCVR T+
Sbjct: 5   LSTNSRWIID-EKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFTW 64

Query: 88  ATHMFTRYA---NRTVEENFDLLDLKQAKVGLAQYNPFVLNKTIVEAYEAVVDVLGATGL 147
              + T      N TV ++F  L L     G    NP +++  ++EAY+ VV  LG   +
Sbjct: 65  PLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNNV 124

Query: 148 MVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNEI 207
           MVI DNH+++P WCC  +DGNGFFG+ +FDP  W+ GL+ +A  F   + VVGMSLRNE+
Sbjct: 125 MVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNEL 184

Query: 208 RGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYDNDLRCLKEKPLNVSTLDNKLVF 267
           RG  +N +DW  Y+ QG   +H  NP VLVI+SGL+YD DL  ++ + +N+ T   KLVF
Sbjct: 185 RGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLVF 244

Query: 268 EVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFVIEGSNPFPLFVSEYGYDQREVD 327
           E+H YSF+  + + +  +  N  C +I+ +  +   F +     FP+F+SE+G D R  +
Sbjct: 245 ELHRYSFT--NTNTWSSKNPNEACGEILKSIENGGGFNL---RDFPVFLSEFGIDLRGKN 304

Query: 328 DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLESNWTQIKNPNFVQ 387
             +NR++ C     A+ D+DW++WT QGSYY REG   ++E +G+L+S+W ++++ +F+Q
Sbjct: 305 VNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFLQ 364

Query: 388 KFQLLQTMLQDPNSNASFSYVIYHPQSGQC-IEVSNDNKEIFLTNCSTSSRWNHDNDSTP 447
           +  L+ + LQ P S +    +++HP +G C ++   D  ++ L  C+ S  W++   +T 
Sbjct: 365 RLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNT- 424

Query: 448 IRMSSTGLCLKASGEGLEASLS-TDCLGKQ-SVWSAISNSKLHLAIVTEDGKSLCLQIES 507
           + +    LCL+++G      LS T C     S W  IS S + LA       SLCL ++ 
Sbjct: 425 LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLA-AKSTNNSLCLDVDE 484

Query: 508 SNSSKIVTNSCICT-NDDPTCLQDTQSQWFELV 534
           +N+  ++ ++C C   +D +C  D  SQWF++V
Sbjct: 485 TNN--LMASNCKCVKGEDSSC--DPISQWFKIV 504

BLAST of PI0012625 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 357.1 bits (915), Expect = 2.5e-98
Identity = 197/538 (36.62%), Postives = 303/538 (56.32%), Query Frame = 0

Query: 1   MERTIQVIPLLALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLI 60
           M + I +   L L L L S   A    L T+ RWI++++ G RVKL C NWPSH + ++ 
Sbjct: 1   MAKAIALTVFLFLFLSLISLTLATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVA 60

Query: 61  EGLNHRPLKELAEEAIKLRFNCVRLTYATHMF---TRYANRTVEENFDLLDLKQAKVGLA 120
           EGL+ +P+  ++++   + FNCVRLT+   +    T   N TV+++F+   L     G+ 
Sbjct: 61  EGLSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIY 120

Query: 121 QYNPFVLNKTIVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQE 180
            +NP+++N  ++  ++AVV  LG   +MVI DNH + P WCCS DD + FFG+  F+P  
Sbjct: 121 THNPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDL 180

Query: 181 WLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVS 240
           W+ GL  +A  F N   VVGMSLRNE+RG    + DW  Y+ +G   +H  NP VLVI+S
Sbjct: 181 WMLGLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILS 240

Query: 241 GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFID 300
           GLN+D DL  LK++P+N+S    KLV E+H YSF+ D   ++    +N+ C+++ +    
Sbjct: 241 GLNFDADLSFLKDRPVNLS-FKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERR 300

Query: 301 HAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 360
              FV++    FPLF+SE+G DQR  D   NR+M+C  A  A+KDLDWA+W   G YY+R
Sbjct: 301 TGGFVLD--QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFR 360

Query: 361 EGQAELAETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEV 420
           EG+  + E +G+L++NW  + N  ++++  ++Q     P    +    I+HP +G C+  
Sbjct: 361 EGKRGVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVR 420

Query: 421 SN--DNKEIFLTNCSTSSRWNHDNDS-TPIRMSSTGLCLKASGEGLEASLSTDCLGKQSV 480
            +     E+ L  C+    W++ +     IR          +  G    L   C   +  
Sbjct: 421 KSHCHESELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICTKIEQ- 480

Query: 481 WSAISNSKLHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFEL 533
              IS +K+HL+  T DG  +CL ++S N+  +V NSC C   D TC  +  SQWF++
Sbjct: 481 ---ISATKMHLSFNTSDGSLVCLDVDSDNN--VVANSCNCLTGDTTC--EPASQWFKI 525

BLAST of PI0012625 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 2.3e-83
Identity = 184/531 (34.65%), Postives = 287/531 (54.05%), Query Frame = 0

Query: 11  LALVLVLFSSLSAYSLLLSTRGRWIIDSQTGQRVKLVCVNWPSHTQSMLIEGLNHRPLKE 70
           L  + +  +S    S  LST+ RWI+D + GQRVKL CVNWP+H Q  + EGL+ +PL  
Sbjct: 9   LFFLFISSTSKLTTSYPLSTKSRWIVD-EKGQRVKLACVNWPAHLQPTVAEGLSKQPLDS 68

Query: 71  LAEEAIKLRFNCVRLTYATHMFTR---YANRTVEENFDLLDLKQAKVGLAQYNPFVLNKT 130
           ++++ + + FNCVRLT+   + T        TV+++F+ L L +  +G+  +NP +L+  
Sbjct: 69  ISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLP 128

Query: 131 IVEAYEAVVDVLGATGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQ 190
           +  A++ VV  LG  G+MVI DNH++ P WCC  +D + FFG  +FDP  W +GL  +A 
Sbjct: 129 LFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMAT 188

Query: 191 RFNNKSTVVGMSLRNEIRGMMENANDWNNYVTQGVTIIHNINPAVLVIVSGLNYDNDLRC 250
            F N + V+GMSLRNE RG  +  + W  ++ QG   +H  NP +LVI+SG+++D +L  
Sbjct: 189 LFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSF 248

Query: 251 LKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICAKIMNNFIDHAEFVIEGSN 310
           L+++ +NVS  D KLVFE+H YSFS D    + +   N+ C KI+     +  F++    
Sbjct: 249 LRDRSVNVSFTD-KLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGGFLL--GR 308

Query: 311 PFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETF 370
            FPL +SE+G DQR  D + NR+M+C  A  A+ DLDWA+W   G YY R G        
Sbjct: 309 GFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTGPG------ 368

Query: 371 GVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCI--EVSNDNKEIF 430
                                       PN N     +++HP +G C+    S++   + 
Sbjct: 369 --------------------------LRPNKN-----LLFHPSTGLCVTNNPSDNIPTLR 428

Query: 431 LTNCSTSSRWNHDNDSTPIRMSSTGLCLKAS---GEGLEASLSTDCLGKQSVWSAISNSK 490
           L  C  S  W  +     + ++   +C++A    G+ ++  + T C    S    IS +K
Sbjct: 429 LGPCPKSDPWTFNPSEGILWINK--MCVEAPNVVGQKVKLGVGTKC----SKLGQISATK 488

Query: 491 LHLAIVTEDGKSLCLQIESSNSSKIVTNSCICTNDDPTCLQDTQSQWFELV 534
           +HL+  T +G  LCL ++  ++S +V N C     D +C  D  SQWF+++
Sbjct: 489 MHLSFKTSNGLLLCLDVDERDNS-VVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA01.3e-11541.59Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P194871.2e-1223.50Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
P235486.0e-1221.03Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0K8533.3e-29593.51Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3... [more]
A0A5A7TMC99.0e-27786.94Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7TMX08.4e-27586.59Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7THG11.1e-27486.38Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1JZU82.4e-27486.62Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=... [more]
Match NameE-valueIdentityDescription
KAE8646606.16.8e-29593.51hypothetical protein Csa_005432 [Cucumis sativus][more]
XP_011658389.16.8e-29593.51glycosyl hydrolase 5 family protein [Cucumis sativus][more]
KAA0042711.11.8e-27686.94major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
XP_023533776.12.7e-27587.36uncharacterized protein LOC111795530 [Cucurbita pepo subsp. pepo][more]
KAA0042709.11.7e-27486.59major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G13130.12.1e-11641.10Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.12.8e-10540.49Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.11.4e-10438.60Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.12.5e-9836.62Glycosyl hydrolase superfamily protein [more]
AT5G16700.12.3e-8334.65Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.80.10.50coord: 406..531
e-value: 8.2E-6
score: 28.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 26..397
e-value: 3.0E-66
score: 226.0
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 10..534
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 64..351
e-value: 4.6E-26
score: 91.9
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 28..387
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 399..515

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0012625.1PI0012625.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds