PI0012351 (gene) Melon (PI 482460) v1

Overview
NamePI0012351
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptiontRNA N6-adenosine threonylcarbamoyltransferase
Locationchr07: 14412650 .. 14415891 (+)
RNA-Seq ExpressionPI0012351
SyntenyPI0012351
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCCTTGGTCCTGACTTCCTTCTCATCATCACTCTCTCATTTCCTACAGCATTGCACCGTTCAGTCCTTCCAATCCCCACAGCAGGCCGTTCTTCACGGCAATCGTAGCGGCACCTTCCCTCCGTTCTTCGCTCATACCTTTTTTCTGCTTCTCCGTCTATCGAAATCCGGCAAGGCATAAACAAAGGATTCGTGTCCGTGTTCCTTTCCAAATATAGGCCACGCACGATCATCCTCCCTCCAATCCAGAATCGCAGACCAGCAATATATATATATCTGGCCGAAATTCCAAAAATTAGTAGACAAAATTCCGAAGTGCTCAAACTGTAGACAACCAAACTAATCAGAGAATCGTTCTGACGAATTGGAGTTCGTTCGGTCGGTTGATGGTTCAAAGTAGGCTAGAGTCGACGACCTAGTAACGTTGCCTCGGATATCTGGGGGATTAGGAACCAGATACAGTTTAAAGAACCGAGCCAAAGAAAGGGTTTCAACCCTTTTTTGTTAGTTCTACTTTCTCTCTTTTATTTCTTCGTAACCGTTTTATATTTTGATTGATTGATATGCATCTCAGTAGTTGAAAATTTTTCAGCGAAGCTTTTTTTTTTTTGCTTAGTTTAGGGGGTTTTGCGTTTTGATTATCATACATGCTTGAAATTTCATGTGCTACATTTTCAGAAACTCTTGTATTTTGTCAGTTAAAACTCATAGCCGTTTATTGTTATCCCTATGGCCTTCATGTTTTTTAGAGTTTTTTTTTCTTCTTTTCCAATTTTCTATAAGGTCCCTTGAATCGATTGGAAAATGAAGAAAGTGATAGCTCTGGGTTTTGAGGGTTCGGCAAACAAGATCGGTGTTGGGGTTGTAACTTTAGATGGCAACATTTTGTCAAATCCACGTCACACCTACATTACACCTCCTGGACATGGCTTCCTTCCTCGTGAAACTGCACAACACCATCTCCACCACATTCTTCCATTGGTTATGTCTGCTCTGGAAACTGCAAAATTACTCCAAAGGATATTGACTGCCTTTGCTACACTAAAGGGCCTGGCATGGGAGCTCCTCTACAGGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTATTGTTGCTGTTAATCACTGTGTTGCACATATTGAGATGGGAAGGGTTGTAACTGGTGCAGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTATAGTGAAGGAAGGTATAGAATCTTTGGGGAGACTATTGATATCGCTGTTGGAAATTGCTTGGACCGGTTTGCAAGAGTCCTAACACTGTCCAATGATCCAAGTCCTGGATACAATATTGAACAGGTGTGTTACAGGAAAATGATAATATAGTTTTTTTTTAATCCATTATTCCTTACGTTGTTTGTCTTCTATTTGTATTTGATACATGATATCTAAAGAAGGAAGAAGGAAGAAGGAAAGGCCAATTTATTTTTGTTTGTGTGACTACTGTTACTTGATCTATTCAGATTCTTATATAACAGAGTTTCAAGTGGTCTGCAGTAATCACTTCATTGTAGGAGGATGTTGAACATATGAAAATCTTCTTTTATCATTGAAGTTGATCAATGTATTATACGGTTCATTCATTGCTTTTCATTTAACAATTTGTTTTTCTACACTTTCTATTGACTATTGTATTAAAGCATGGTTTCATTGGTTTAAGATCTCACATGTGAAGGAGGCCAATGTCTTCAGGTTTCTCTATTTGTTAGTCTAATGTTTCAAATGGTTCCAAGGTTTTCAACAATTTGTATGATTTGCATGCGGTCCTTATAAGTTGATTCTTGTTCTTTACTTATCATGATATAAATTATTCAAAATGTGTTTTAGCTTGAAACCGTTTTTCTCTTTCCTGAATCCCAACAAGGGCTTGAGCTTAACTAATGTGTAAGAAATGCTTTTGTTGTCTCATTCTATTGATTTTTCTTTGTCCACAGCTTGCAAAGAAAGGAAAACTTTTTATTGAACTTCCATATGTTGTCAAAGGGATGGATGTATCATTCAGTGGGATTTTGAGCTATATTGAAGCAACTGCCGAGGAGAAGCTAAAAAGTAATGAGTGTACTCCTGCTGACTTGTGCTACTCTCTGCAGGTAATCTTTGTTTACCATGATAAAAGCGGATTTTGTCTCCATTTCTGAGATGGGTTGAAAAGGTTGGACCATTCTGTAACTAAGCTGTATTTTTGAATTGGGTTGTGCTCTTTTATAGATGGTCTCAAACTTCCATCCAACTTGTAAAGATGCTATGTACACTCATTCTTCTGTAAAATGTCTTGCAATTGATATTTCATTGACTTTTCACTTCCTACTTGGTTGTAGGAGACTTTGTTTGCTATGCTTGTGGAAATAACGGAAAGAGCCATGGCTCACTGTGACAAGAAAGATGTTCTTATAGTCGGTGGTGTAGGTTGTAACGAGCGCTTGCAAGAGATGATGAGAATAATGTGCTCTGAGAGAGGTGGGAGGTTATTTGCAACTGATGACCGTTATTGTATCGATAATGGGGCAATGATTGCATATACTGGTCTTCTTGCTTGGGCACATGGTGTTTCGACACCATTGGAGGAAGTAACATTCACCCAACGGTTTCGAACTGATGAAGTACATGCAATCTGGAGAGAAAAGGCTTTGACTAATGGAAAGGAGCTTATGGAAGATGTCTAAGCCATCTTTGTTCTCTTCAAAAGGTGATAGTTAGTGTGGCAATGATATGTTTTGTAAGCTTAGTGTAACTGGCATAATTCTATTTAGATTTTCCATGATGCCTGAAGTTTTATAGATAAGCAGCATCAATAAAAAATTTAGCCAAGTGGTCTTGTAGGATATAACTTGTAAACGGTATTTACAGGTCCAATGTAATTAGAAATCGGCAAACGAACGCTAACTCTGAAGGCAACTTAGTTCAGCATCTGCAGTTCATTCTTTCTGGTGTATTGCGCACTTGGGTATTCTTGAAATACTCTTTTTTTTGTTATAAAATTATCGTTATTATTTTTTTTCGTTCAAAGAAGATTGAAGTTTGATGATAATTGCAGCTATGCATGGATAGACAAAAACAAATGTTTTTGCTTAAGTTTTGTACTCTCTTTCTATTGATCGTTTGTCATTGAAATTTTGATGGCCAATGGAATGAACAAATGTATTTATTACTAATTGAGAAGTAGGGTGTACATTCACTAAAATA

mRNA sequence

AGCCTTGGTCCTGACTTCCTTCTCATCATCACTCTCTCATTTCCTACAGCATTGCACCGTTCAGTCCTTCCAATCCCCACAGCAGGCCGTTCTTCACGGCAATCGTAGCGGCACCTTCCCTCCGTTCTTCGCTCATACCTTTTTTCTGCTTCTCCGTCTATCGAAATCCGGCAAGGCATAAACAAAGGATTCGTGTCCGTGTTCCTTTCCAAATATAGGCCACGCACGATCATCCTCCCTCCAATCCAGAATCGCAGACCAGCAATATATATATATCTGGCCGAAATTCCAAAAATTAGTAGACAAAATTCCGAAGTGCTCAAACTGTAGACAACCAAACTAATCAGAGAATCGTTCTGACGAATTGGAGTTCGTTCGGTCGGTTGATGGTTCAAAGTAGGCTAGAGTCGACGACCTAGTAACGTTGCCTCGGATATCTGGGGGATTAGGAACCAGATACAGTTTAAAGAACCGAGCCAAAGAAAGGGTTTCAACCCTTTTTTGTCCCTTGAATCGATTGGAAAATGAAGAAAGTGATAGCTCTGGGTTTTGAGGGTTCGGCAAACAAGATCGGTGTTGGGGTTGTAACTTTAGATGGCAACATTTTGTCAAATCCACGTCACACCTACATTACACCTCCTGGACATGGCTTCCTTCCTCGTGAAACTGCACAACACCATCTCCACCACATTCTTCCATTGGTTATGTCTGCTCTGGAAACTGCAAAATTACTCCAAAGGATATTGACTGCCTTTGCTACACTAAAGGGCCTGGCATGGGAGCTCCTCTACAGGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTATTGTTGCTGTTAATCACTGTGTTGCACATATTGAGATGGGAAGGGTTGTAACTGGTGCAGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTATAGTGAAGGAAGGTATAGAATCTTTGGGGAGACTATTGATATCGCTGTTGGAAATTGCTTGGACCGGTTTGCAAGAGTCCTAACACTGTCCAATGATCCAAGTCCTGGATACAATATTGAACAGCTTGCAAAGAAAGGAAAACTTTTTATTGAACTTCCATATGTTGTCAAAGGGATGGATGTATCATTCAGTGGGATTTTGAGCTATATTGAAGCAACTGCCGAGGAGAAGCTAAAAAGTAATGAGTGTACTCCTGCTGACTTGTGCTACTCTCTGCAGGAGACTTTGTTTGCTATGCTTGTGGAAATAACGGAAAGAGCCATGGCTCACTGTGACAAGAAAGATGTTCTTATAGTCGGTGGTGTAGGTTGTAACGAGCGCTTGCAAGAGATGATGAGAATAATGTGCTCTGAGAGAGGTGGGAGGTTATTTGCAACTGATGACCGTTATTGTATCGATAATGGGGCAATGATTGCATATACTGGTCTTCTTGCTTGGGCACATGGTGTTTCGACACCATTGGAGGAAGTAACATTCACCCAACGGTTTCGAACTGATGAAGTACATGCAATCTGGAGAGAAAAGGCTTTGACTAATGGAAAGGAGCTTATGGAAGATGTCTAAGCCATCTTTGTTCTCTTCAAAAGGTGATAGTTAGTGTGGCAATGATATGTTTTGTAAGCTTAGTGTAACTGGCATAATTCTATTTAGATTTTCCATGATGCCTGAAGTTTTATAGATAAGCAGCATCAATAAAAAATTTAGCCAAGTGGTCTTGTAGGATATAACTTGTAAACGGTATTTACAGGTCCAATGTAATTAGAAATCGGCAAACGAACGCTAACTCTGAAGGCAACTTAGTTCAGCATCTGCAGTTCATTCTTTCTGGTGTATTGCGCACTTGGGTATTCTTGAAATACTCTTTTTTTTGTTATAAAATTATCGTTATTATTTTTTTTCGTTCAAAGAAGATTGAAGTTTGATGATAATTGCAGCTATGCATGGATAGACAAAAACAAATGTTTTTGCTTAAGTTTTGTACTCTCTTTCTATTGATCGTTTGTCATTGAAATTTTGATGGCCAATGGAATGAACAAATGTATTTATTACTAATTGAGAAGTAGGGTGTACATTCACTAAAATA

Coding sequence (CDS)

ATGGGAGCTCCTCTACAGGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTATTGTTGCTGTTAATCACTGTGTTGCACATATTGAGATGGGAAGGGTTGTAACTGGTGCAGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTATAGTGAAGGAAGGTATAGAATCTTTGGGGAGACTATTGATATCGCTGTTGGAAATTGCTTGGACCGGTTTGCAAGAGTCCTAACACTGTCCAATGATCCAAGTCCTGGATACAATATTGAACAGCTTGCAAAGAAAGGAAAACTTTTTATTGAACTTCCATATGTTGTCAAAGGGATGGATGTATCATTCAGTGGGATTTTGAGCTATATTGAAGCAACTGCCGAGGAGAAGCTAAAAAGTAATGAGTGTACTCCTGCTGACTTGTGCTACTCTCTGCAGGAGACTTTGTTTGCTATGCTTGTGGAAATAACGGAAAGAGCCATGGCTCACTGTGACAAGAAAGATGTTCTTATAGTCGGTGGTGTAGGTTGTAACGAGCGCTTGCAAGAGATGATGAGAATAATGTGCTCTGAGAGAGGTGGGAGGTTATTTGCAACTGATGACCGTTATTGTATCGATAATGGGGCAATGATTGCATATACTGGTCTTCTTGCTTGGGCACATGGTGTTTCGACACCATTGGAGGAAGTAACATTCACCCAACGGTTTCGAACTGATGAAGTACATGCAATCTGGAGAGAAAAGGCTTTGACTAATGGAAAGGAGCTTATGGAAGATGTCTAA

Protein sequence

MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMDVSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFTQRFRTDEVHAIWREKALTNGKELMEDV
Homology
BLAST of PI0012351 vs. ExPASy Swiss-Prot
Match: A7SXZ6 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Nematostella vectensis OX=45351 GN=osgep PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 9.4e-107
Identity = 185/254 (72.83%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPL   AV  R ++Q+W KPI+ VNHC+ HIEMGR++TGA++P VLYVSGGNTQVIAY
Sbjct: 82  MGAPLVAVAVVARTVAQLWKKPIIGVNHCIGHIEMGRLITGANNPTVLYVSGGNTQVIAY 141

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
            + RYRIFGETIDIAVGNCLDRFARVL LSNDPSPGYNIEQ+AKKGK  IELPY VKGMD
Sbjct: 142 LQKRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGYNIEQMAKKGKKLIELPYTVKGMD 201

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIE  A + L S ECTP DLC+SLQET+FAMLVE TERAMAHC   +VLIVG
Sbjct: 202 VSFSGILSYIECMAHKLLSSEECTPEDLCFSLQETVFAMLVETTERAMAHCGSNEVLIVG 261

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCN+RLQEMM +M  ERG +L+ATD+R+CIDNGAMIA  G   +  G  TPLE+ T T
Sbjct: 262 GVGCNKRLQEMMDVMAKERGAKLYATDERFCIDNGAMIAQAGWEMFQTGSVTPLEQTTCT 321

Query: 241 QRFRTDEVHAIWRE 255
           QRFRTDEV   WR+
Sbjct: 322 QRFRTDEVEVTWRD 335

BLAST of PI0012351 vs. ExPASy Swiss-Prot
Match: Q0VCI1 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Bos taurus OX=9913 GN=OSGEP PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 4.7e-106
Identity = 183/254 (72.05%), Postives = 208/254 (81.89%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPL   AV  R ++Q+WNKP++ VNHC+ HIEMGR++TGA +P VLYVSGGNTQVIAY
Sbjct: 82  MGAPLVSVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGATNPTVLYVSGGNTQVIAY 141

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+AK+GK  +ELPY VKGMD
Sbjct: 142 SEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMD 201

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILS+IE  A+  L + ECTP DLC+SLQET+FAMLVEITERAMAHC  ++ LIVG
Sbjct: 202 VSFSGILSFIEDVAQRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSQEALIVG 261

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCN RLQEMM  MC ERG RLFATD+R+CIDNGAMIA  G   +  G  TPL E   T
Sbjct: 262 GVGCNVRLQEMMETMCQERGARLFATDERFCIDNGAMIAQAGWEMFQAGHRTPLSESGIT 321

Query: 241 QRFRTDEVHAIWRE 255
           QR+RTDEV   WR+
Sbjct: 322 QRYRTDEVEVTWRD 335

BLAST of PI0012351 vs. ExPASy Swiss-Prot
Match: Q9NPF4 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Homo sapiens OX=9606 GN=OSGEP PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 1.8e-105
Identity = 183/254 (72.05%), Postives = 206/254 (81.10%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPL   AV  R ++Q+WNKP+V VNHC+ HIEMGR++TGA  P VLYVSGGNTQVIAY
Sbjct: 82  MGAPLVSVAVVARTVAQLWNKPLVGVNHCIGHIEMGRLITGATSPTVLYVSGGNTQVIAY 141

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+AK+GK  +ELPY VKGMD
Sbjct: 142 SEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMD 201

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILS+IE  A   L + ECTP DLC+SLQET+FAMLVEITERAMAHC  ++ LIVG
Sbjct: 202 VSFSGILSFIEDVAHRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSQEALIVG 261

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCN RLQEMM  MC ERG RLFATD+R+CIDNGAMIA  G   +  G  TPL +   T
Sbjct: 262 GVGCNVRLQEMMATMCQERGARLFATDERFCIDNGAMIAQAGWEMFRAGHRTPLSDSGVT 321

Query: 241 QRFRTDEVHAIWRE 255
           QR+RTDEV   WR+
Sbjct: 322 QRYRTDEVEVTWRD 335

BLAST of PI0012351 vs. ExPASy Swiss-Prot
Match: Q9WVS2 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Rattus norvegicus OX=10116 GN=Osgep PE=2 SV=2)

HSP 1 Score: 383.3 bits (983), Expect = 2.3e-105
Identity = 181/254 (71.26%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPL   AV  R ++Q+WNKP++ VNHC+ HIEMGR++TGA +P VLYVSGGNTQVI+Y
Sbjct: 82  MGAPLASVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGAVNPTVLYVSGGNTQVISY 141

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+AK+GK  +ELPY VKGMD
Sbjct: 142 SEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMD 201

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILS+IE  A+  L + ECTP DLC+SLQET+FAMLVEITERAMAHC  K+ LIVG
Sbjct: 202 VSFSGILSFIEDAAQRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSKEALIVG 261

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCN RLQEMM  MC ERG +LFATD+R+CIDNGAMIA  G   +  G  TPL++   T
Sbjct: 262 GVGCNVRLQEMMATMCQERGAQLFATDERFCIDNGAMIAQAGWEMFQAGHRTPLQDSGIT 321

Query: 241 QRFRTDEVHAIWRE 255
           QR+RTDEV   WR+
Sbjct: 322 QRYRTDEVEVTWRD 335

BLAST of PI0012351 vs. ExPASy Swiss-Prot
Match: Q8BWU5 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Mus musculus OX=10090 GN=Osgep PE=1 SV=2)

HSP 1 Score: 382.5 bits (981), Expect = 3.9e-105
Identity = 180/254 (70.87%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPL   AV  R ++Q+WNKP++ VNHC+ HIEMGR++TGA +P VLYVSGGNTQVI+Y
Sbjct: 82  MGAPLASVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGAVNPTVLYVSGGNTQVISY 141

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+AK+GK  +ELPY VKGMD
Sbjct: 142 SEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQMAKRGKKLVELPYTVKGMD 201

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILS+IE  A+  L + ECTP DLC+SLQET+FAMLVEITERAMAHC  K+ LIVG
Sbjct: 202 VSFSGILSFIEDAAQRMLATGECTPEDLCFSLQETVFAMLVEITERAMAHCGSKEALIVG 261

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCN RLQEMM  MC ERG +LFATD+R+C+DNGAMIA  G   +  G  TPL++   T
Sbjct: 262 GVGCNLRLQEMMGTMCQERGAQLFATDERFCVDNGAMIAQAGWEMFQAGHRTPLKDSAIT 321

Query: 241 QRFRTDEVHAIWRE 255
           QR+RTDEV   WR+
Sbjct: 322 QRYRTDEVEVTWRD 335

BLAST of PI0012351 vs. ExPASy TrEMBL
Match: A0A5A7T3P8 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=GCP2 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.0e-149
Identity = 264/265 (99.62%), Postives = 265/265 (100.00%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIE+TAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIESTAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT
Sbjct: 265 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 324

Query: 241 QRFRTDEVHAIWREKALTNGKELME 266
           QRFRTDEVHAIWREKALTNGKELME
Sbjct: 325 QRFRTDEVHAIWREKALTNGKELME 349

BLAST of PI0012351 vs. ExPASy TrEMBL
Match: A0A5D3DJA8 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=GCP2 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.3e-148
Identity = 262/266 (98.50%), Postives = 265/266 (99.62%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIE+TAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIESTAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMC+ERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT
Sbjct: 265 GVGCNERLQEMMRIMCTERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 324

Query: 241 QRFRTDEVHAIWREKALTNGKELMED 267
           QRFRTDEVHAIWREK LTNGKELME+
Sbjct: 325 QRFRTDEVHAIWREKTLTNGKELMEE 350

BLAST of PI0012351 vs. ExPASy TrEMBL
Match: A0A6J1KKP2 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111494026 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 8.4e-143
Identity = 255/266 (95.86%), Postives = 260/266 (97.74%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGR+VTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRIVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKG+ FIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGEKFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHG+STPLEEVTFT
Sbjct: 265 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGMSTPLEEVTFT 324

Query: 241 QRFRTDEVHAIWREKALTNGKELMED 267
           QRFRTDEVHAIWREK   +  ELME+
Sbjct: 325 QRFRTDEVHAIWREK--KDSTELMEE 348

BLAST of PI0012351 vs. ExPASy TrEMBL
Match: A0A6J1FLZ0 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447025 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 8.4e-143
Identity = 255/266 (95.86%), Postives = 260/266 (97.74%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGR+VTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRIVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKG+ FIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGEKFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHG+STPLEEVTFT
Sbjct: 265 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGMSTPLEEVTFT 324

Query: 241 QRFRTDEVHAIWREKALTNGKELMED 267
           QRFRTDEVHAIWREK   +  ELME+
Sbjct: 325 QRFRTDEVHAIWREK--KDSTELMEE 348

BLAST of PI0012351 vs. ExPASy TrEMBL
Match: A0A6J1C4S6 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Momordica charantia OX=3673 GN=LOC111008475 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 247/255 (96.86%), Postives = 253/255 (99.22%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKP+VAVNHCVAHIEMGR+VTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGRIVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKG+ FIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGEKFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIEATAEEKLK NECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIEATAEEKLKCNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLL+WAHG+STPLEEVTFT
Sbjct: 265 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLSWAHGISTPLEEVTFT 324

Query: 241 QRFRTDEVHAIWREK 256
           QRFRTDEVHAIWRE+
Sbjct: 325 QRFRTDEVHAIWREE 339

BLAST of PI0012351 vs. NCBI nr
Match: KAA0037603.1 (putative tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis melo var. makuwa] >TYK09345.1 putative tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 536.6 bits (1381), Expect = 1.2e-148
Identity = 264/265 (99.62%), Postives = 265/265 (100.00%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIE+TAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIESTAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT
Sbjct: 265 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 324

Query: 241 QRFRTDEVHAIWREKALTNGKELME 266
           QRFRTDEVHAIWREKALTNGKELME
Sbjct: 325 QRFRTDEVHAIWREKALTNGKELME 349

BLAST of PI0012351 vs. NCBI nr
Match: XP_004142777.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis sativus] >KGN51795.2 hypothetical protein Csa_008020 [Cucumis sativus])

HSP 1 Score: 534.6 bits (1376), Expect = 4.7e-148
Identity = 263/266 (98.87%), Postives = 265/266 (99.62%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIE+TAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIESTAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHG STPLEEVTFT
Sbjct: 265 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGDSTPLEEVTFT 324

Query: 241 QRFRTDEVHAIWREKALTNGKELMED 267
           QRFRTDEVHAIWREKALTNGKELME+
Sbjct: 325 QRFRTDEVHAIWREKALTNGKELMEE 350

BLAST of PI0012351 vs. NCBI nr
Match: TYK23309.1 (putative tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 534.6 bits (1376), Expect = 4.7e-148
Identity = 262/266 (98.50%), Postives = 265/266 (99.62%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIE+TAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIESTAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMC+ERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT
Sbjct: 265 GVGCNERLQEMMRIMCTERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 324

Query: 241 QRFRTDEVHAIWREKALTNGKELMED 267
           QRFRTDEVHAIWREK LTNGKELME+
Sbjct: 325 QRFRTDEVHAIWREKTLTNGKELMEE 350

BLAST of PI0012351 vs. NCBI nr
Match: XP_038905629.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase [Benincasa hispida] >XP_038905630.1 probable tRNA N6-adenosine threonylcarbamoyltransferase [Benincasa hispida] >XP_038905631.1 probable tRNA N6-adenosine threonylcarbamoyltransferase [Benincasa hispida])

HSP 1 Score: 516.2 bits (1328), Expect = 1.7e-142
Identity = 253/255 (99.22%), Postives = 254/255 (99.61%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEE TFT
Sbjct: 265 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEATFT 324

Query: 241 QRFRTDEVHAIWREK 256
           QRFRTDEVHAIWRE+
Sbjct: 325 QRFRTDEVHAIWRER 339

BLAST of PI0012351 vs. NCBI nr
Match: XP_022941751.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita moschata] >XP_022999783.1 probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita maxima] >KAG7031304.1 putative tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 516.2 bits (1328), Expect = 1.7e-142
Identity = 255/266 (95.86%), Postives = 260/266 (97.74%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGR+VTGADDPVVLYVSGGNTQVIAY
Sbjct: 85  MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRIVTGADDPVVLYVSGGNTQVIAY 144

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKG+ FIELPYVVKGMD
Sbjct: 145 SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGEKFIELPYVVKGMD 204

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG
Sbjct: 205 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 264

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHG+STPLEEVTFT
Sbjct: 265 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGMSTPLEEVTFT 324

Query: 241 QRFRTDEVHAIWREKALTNGKELMED 267
           QRFRTDEVHAIWREK   +  ELME+
Sbjct: 325 QRFRTDEVHAIWREK--KDSTELMEE 348

BLAST of PI0012351 vs. TAIR 10
Match: AT4G22720.1 (Actin-like ATPase superfamily protein )

HSP 1 Score: 480.3 bits (1235), Expect = 9.9e-136
Identity = 231/255 (90.59%), Postives = 244/255 (95.69%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSA+ VRVLSQ+W KPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY
Sbjct: 86  MGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 145

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPGYNIEQLAKKG+ FI+LPY VKGMD
Sbjct: 146 SEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGYNIEQLAKKGENFIDLPYAVKGMD 205

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIE TAEEKLK+NECTPADLCYSLQET+FAMLVEITERAMAHCDKKDVLIVG
Sbjct: 206 VSFSGILSYIETTAEEKLKNNECTPADLCYSLQETVFAMLVEITERAMAHCDKKDVLIVG 265

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMR MCSER G+LFATDDRYCIDNGAMIAYTGLLA+ +G+ TP+E+ TFT
Sbjct: 266 GVGCNERLQEMMRTMCSERDGKLFATDDRYCIDNGAMIAYTGLLAFVNGIETPIEDSTFT 325

Query: 241 QRFRTDEVHAIWREK 256
           QRFRTDEVHA+WREK
Sbjct: 326 QRFRTDEVHAVWREK 340

BLAST of PI0012351 vs. TAIR 10
Match: AT4G22720.2 (Actin-like ATPase superfamily protein )

HSP 1 Score: 480.3 bits (1235), Expect = 9.9e-136
Identity = 231/255 (90.59%), Postives = 244/255 (95.69%), Query Frame = 0

Query: 1   MGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 60
           MGAPLQVSA+ VRVLSQ+W KPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY
Sbjct: 86  MGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGRVVTGADDPVVLYVSGGNTQVIAY 145

Query: 61  SEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGKLFIELPYVVKGMD 120
           SEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPGYNIEQLAKKG+ FI+LPY VKGMD
Sbjct: 146 SEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGYNIEQLAKKGENFIDLPYAVKGMD 205

Query: 121 VSFSGILSYIEATAEEKLKSNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVG 180
           VSFSGILSYIE TAEEKLK+NECTPADLCYSLQET+FAMLVEITERAMAHCDKKDVLIVG
Sbjct: 206 VSFSGILSYIETTAEEKLKNNECTPADLCYSLQETVFAMLVEITERAMAHCDKKDVLIVG 265

Query: 181 GVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAWAHGVSTPLEEVTFT 240
           GVGCNERLQEMMR MCSER G+LFATDDRYCIDNGAMIAYTGLLA+ +G+ TP+E+ TFT
Sbjct: 266 GVGCNERLQEMMRTMCSERDGKLFATDDRYCIDNGAMIAYTGLLAFVNGIETPIEDSTFT 325

Query: 241 QRFRTDEVHAIWREK 256
           QRFRTDEVHA+WREK
Sbjct: 326 QRFRTDEVHAVWREK 340

BLAST of PI0012351 vs. TAIR 10
Match: AT2G45270.1 (glycoprotease 1 )

HSP 1 Score: 83.2 bits (204), Expect = 3.5e-16
Identity = 69/243 (28.40%), Postives = 112/243 (46.09%), Query Frame = 0

Query: 5   LQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRVVTG--ADDPVVLYVSGG-NTQVIAYS 64
           L+V     R ++  ++ PIV V+H  AH  + R+V    +   + L +SGG N  V+A+ 
Sbjct: 171 LRVGVRKARRVAGNFSLPIVGVHHMEAHALVARLVEQELSFPFMALLISGGHNLLVLAHK 230

Query: 65  EGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKG-----KLFIELPYVV 124
            G+Y   G T+D A+G   D+ A+ L L    S G  +E+LA +G     K  + + Y  
Sbjct: 231 LGQYTQLGTTVDDAIGEAFDKTAKWLGLDMHRSGGPAVEELALEGDAKSVKFNVPMKY-H 290

Query: 125 KGMDVSFSGILSYIEATAEEKLKSNEC-----------TPADLCYSLQETLFAMLVEITE 184
           K  + S++G+ + +    E K    +C             AD+  S Q      L E  E
Sbjct: 291 KDCNFSYAGLKTQVRLAIEAKEIDAKCPVSSATNEDRRNRADIAASFQRVAVLHLEEKCE 350

Query: 185 RAM-----AHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAY 224
           RA+          K ++I GGV  N+ ++  +  +   +  +L       C DNG M+A+
Sbjct: 351 RAIDWALELEPSIKHMVISGGVASNKYVRLRLNNIVENKNLKLVCPPPSLCTDNGVMVAW 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7SXZ69.4e-10772.83Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Nematostella vectensi... [more]
Q0VCI14.7e-10672.05Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Bos taurus OX=9913 GN... [more]
Q9NPF41.8e-10572.05Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Homo sapiens OX=9606 ... [more]
Q9WVS22.3e-10571.26Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Rattus norvegicus OX=... [more]
Q8BWU53.9e-10570.87Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Mus musculus OX=10090... [more]
Match NameE-valueIdentityDescription
A0A5A7T3P86.0e-14999.62Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucumis melo var. mak... [more]
A0A5D3DJA82.3e-14898.50Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucumis melo var. mak... [more]
A0A6J1KKP28.4e-14395.86Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucurbita maxima OX=3... [more]
A0A6J1FLZ08.4e-14395.86Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucurbita moschata OX... [more]
A0A6J1C4S61.0e-14096.86Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Momordica charantia O... [more]
Match NameE-valueIdentityDescription
KAA0037603.11.2e-14899.62putative tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis melo var. makuw... [more]
XP_004142777.14.7e-14898.87probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis sativus] >KGN51... [more]
TYK23309.14.7e-14898.50putative tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis melo var. makuw... [more]
XP_038905629.11.7e-14299.22probable tRNA N6-adenosine threonylcarbamoyltransferase [Benincasa hispida] >XP_... [more]
XP_022941751.11.7e-14295.86probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita moschata] >XP... [more]
Match NameE-valueIdentityDescription
AT4G22720.19.9e-13690.59Actin-like ATPase superfamily protein [more]
AT4G22720.29.9e-13690.59Actin-like ATPase superfamily protein [more]
AT2G45270.13.5e-1628.40glycoprotease 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017861Kae1/TsaD familyPRINTSPR00789OSIALOPTASEcoord: 14..33
score: 43.04
coord: 46..58
score: 57.19
coord: 176..185
score: 55.65
coord: 68..89
score: 50.4
NoneNo IPR availableGENE3D3.30.420.40coord: 201..257
e-value: 1.1E-12
score: 49.9
NoneNo IPR availableGENE3D3.30.420.40coord: 44..200
e-value: 2.6E-47
score: 162.7
IPR000905Gcp-like domainPFAMPF00814TsaDcoord: 1..220
e-value: 2.4E-68
score: 230.8
IPR000905Gcp-like domainPANTHERPTHR11735TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASEcoord: 1..255
IPR034680tRNA N6-adenosine threonylcarbamoyltransferase Kae1, archaea and eukaryotePANTHERPTHR11735:SF14TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE-RELATEDcoord: 1..255
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 23..220

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0012351.1PI0012351.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002949 tRNA threonylcarbamoyladenosine modification
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000408 EKC/KEOPS complex
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061711 N(6)-L-threonylcarbamoyladenine synthase activity
molecular_function GO:0008194 UDP-glycosyltransferase activity