PI0012342 (gene) Melon (PI 482460) v1

Overview
NamePI0012342
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionSubtilisin-like protease
Locationchr04: 29773749 .. 29778610 (-)
RNA-Seq ExpressionPI0012342
SyntenyPI0012342
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCACCATGGCCACTGCTGTTATTTCAGTCCGCCACCATTTGAATTACCATGGCGCACTACGTGTGGATAAGGGAAAAGCTTGGCATTTTGGCAGTGCGCGTGCTCTGATCATGAAACCCACCAACTGATCATCTCTCATTGAATTTTTCCCCATATTCCTTCTTCACCACCAGTTTTCATCTGTAAAAACATATAAATTAGCGCTTGAAGTCGAAGGGAACTTACTCAATTAGATCAATACAGAAGCCAATTAGCCAAGCCAATACGCCATGCAAAGCATTTCTTTTTGTGTCTTTTTCGCTTTGGTTTGTGTCATCTTTTTTCTTGTTTCTGAAAATGTTAAAGTAGCTGATGCTGCTGAAGATGCAAAAAATGGTGTTTATGTCGTTTACATGGGATCCGCTTCTGGCTCCAGGACTGACTTCCTTCGCCTTCTTAACTCTGTCAACAGACGGTAACATTATAATATATCTCAATTCCTTACGCTAATGGACCTATTTCCTCTCAATTTAATTCATTAATATTCATCTTTTCTCTAAAGTAAACATTCTAAGTTCAATTTTATTTTATTTTTTTTAAAAAAAGAAGTTTATTGGTTTTCAAAAATCAAACTTACACCTATTTTCTCATTCTTTTTTACAAATTTTTATATTTAAAAGTGTTGAATTCTTAATCGAATTAAAAAAAAACAAAAATAAATATTGAATTTTTTAATCAAAATTTGACTTAGTCCTAAAATGTAGCCAATAAAACATAGAAACCAATAGATGAAAGTAGAATTTGTAGACTTAATTTTAAAATATATGATTATTAAACAAAGCCTAAATAATCACCGAACGAAACTTTAAAATTACAACTACATAGTAAGTTCTTAAATTTTCAATTGTGAGCCTAAATAGTTATCAAAACCTAACCTTACTATATAATAAATTCTTAAGATTACAATTATTAGCCTAAGTAGTTATAAAACCGGAGCCTTAAAATTAAAACTACATGATAAGTTCTTAAATTATCAATTAACTAAGAATTTCTTGACAATGCATTATAAATTTACTCGACACAATAAAAACTATGTAATATTAGATAACAAAGGAAAACCGATGTTGGACTATAGAGATTGAAAAGGCAGAGAGGAATTAAAAACTCACATATTATTTTGTTGTTTCGTTGTGTGGAACGTAGGAACGCAGTGGTACACACATATAAACATGGGTTCACAGGATTTGCAGCTCGTCTATCAGAACATGAAGCCCAAGCCATGAGACAAAACCCTGGAGTTGTTTCGGTTTTTCCAGATCCAATATTGAAGTTACACACAACTCATTCATGGGATTTCTTGGTCAGTCAGACAAGCGTCAAAATCGACGCCAATCCCAAATCAGATCCCCCAACCTCTTCCTCTCAACCATACGATACCATTATAGGAATCTTGGACACTGGTAAATATATATATCAAACATTAATTATTCTCTGCTCTGCAGTCTGCACTAATGATAATATATAAGAAGTTAGTTCTAAATAGTCACCGGTAAAATTAGGTATCTGGCCGGAATCGGAGAGTTTTAATGACGAGGGTATGGGTCCGATACCGTCGAGGTGGAAAGGTACCTGCATGGTAGGCGATGACTTCACTTCCTCCAACTGTAATAGGTGTGTGTGTGCCCCCCACAACTGACACTCTGGTTTACTACCAAGTTTAAAGTTTAGTCTTTTTAAATCATGATATTTATTTTAGTGTTTTTATATTATGATTATTGTTTAAATTACGAACATACTCTATGAACGTGAGTTTTGTTTTATTTAGTATGTTTTGTGTTTTTTTTTAATTTATGGACTTTTAATTTTGTGTAATTATAAACCTATGAACTTTCTATTTTGTTATTATAGTTCTGAAAATCAGTTGATTTATTGAATATAAATCTAAATCAATACATCTATGAATTTTTAAAAAATATATATCAAAATTCAAAGGCTAATATTGTAACTATAAAAATTTAAGAATTAAATAAACATAAATCTGAAATCTTAAGAATGGTTTATTTTTCCAATTATTTTTTTAGTTTTTAATGCTTAAAAAAATATACTCTTTAAGTTTAAAAAAATGTTTTTAAACTCTTTAAAATTAACATAGGCCCTAAATTTGTAGTTTAACTTAAGTTTTAACTTTTCTTTTTTAAGTGTCTTAATCATTTCATGAAATTTTTGTCAATAAGATAGCTATTGCCTATTATGTAATTCACTGACTATAGAATATAATTTAAATATAAAAAATTAAAAAATTGAATTATAATGATTAAATTGAAAAAAGATATGCTTTATTAAAAAAATTGAAAAAGTGTGCTGTGTTTTAAAAAACACATGCATGGAGATTAAAATTAAAGCATCGAACCATATTATGTGCAAGTTTGACATGTTTATCGTGCCAGAAAAATCATTGGAGCAAGATTTTACGAAAGTTCTGAGAGCGACGGCATACGGTTCCACTCACCTCGAGACGGAGCTGGACATGGCACCCACGTGGCATCAACGGCGGCCGGAAGTGCCGTGGCTAATGCATCGTACTATGGCCTCGCAGCCGGAACGGCCAAGGGCGGCTCCCCCGGCTCAAGGATCGCCATGTACAGAGTCTGCATGGCCGACGGCTGTCGTGGATCGTCAATTCTGAAGGCGTTTGATGATTCCATAGCCGATGGGGTCGATGTATTGTCCTTATCTCTCGGGACACCCTCCATATTCCGACAAAATATGACGGTCGACCCTATTGCTATCGGAGCCTTTCATGCGGTTGAGAAGGGCATTACAGTTGTCTGCTCCGCCGGAAACGACGGTCCCAGTTATGGAACGGTGGTGAACGACGCGCCGTGGATTCTAACAGTGGCCGCCTCCACCATTGATAGGGATTTTGAGTCTGATGTTGTGTTGGGCAACAAAAAAGTGATCAAGGTAATTCAAATTCCTTCAGCAACTGCAATGAAAATAGCTAAAACCACATTAAATTATTTCAAATAAAAAAAATTATAACGCAGGGCGAAGGTATAAATTTCTCTGATCTTCAAAAGTCTCCTGTATACCCACTGATACAAGGCAAGTCAGCCAAGAAAGCGAGTGCCAGTGAAGACAGTGCAAGGTATATAAGAAATGACATGCTGAGTAATTTATTAAGATTTCTTACAAAGCTTTCTGATGAGTTCTCCGGTTGTTATGTCTCAATTTAATTAGGATTTGTTCTGAAGACTCCATGGATGAAGCTCAAGTGAAGAGGAAGATAGTTATTTGCGAAAGCAATGTCGAAGGAGGCGGTTCGGATTGGCAAAGTCAGGCTGAAGCAGTGAAGAATCTTGGAGGGGTTGGGGTAGTTTTGATTGATGACGACTCAAAATTAGTTGCAGAGAAGTTTCCTACTCCCATGACAGTTATTAGCAAAAAGGATGGTCCTGAGATCCTCTCCTACGTTAACTCAAGCAGGTAAAAGTTAAAATTATCGTCTATTTAATCAATCTAGATAGAAAGCTTGGATGTGAAGTGAGCTTCAATTCCATATCTTCTTTAGTAGCTTGAACTTCATGATCTTAAATGGCTAATTGAGTTTGAAATTTTCTGAAGGAAACCAGTTGCTACAATTCTCCCCACTGAGACCATAATAAACTATAAGCCAGCACCTGCTATAACATATTTTTCATCCAGAGGGCCTAATCCCGAAGTATTAAACATAATCAAGGTAAAAGGCAGCTTCTACAGGAAGGACGAGGGGAAAGATTATTATATATTAACTTGAAAATTTCAACCTTCATGAAGAAAATTTATTGTTCCACGCCTGAATTTGAGAGTTATTGTGATGCAGCCGGACATATCAGCACCAGGAGTGAACATTCTTGCAGCCTGGCTTGGCAATGATTCAAGCCCAACCCCACAAGCAACAAAGTCACCACTCTTCAATGTGATCTCAGGAACTTCAATGTCCTGCCCCCATGTCTCTGGCGTAGTGGCCTCTGTAAAATCTCAAAACCCCACATGGAGTCCCTCAGCAATCAAATCAGCTATCATGACAACAGGCAAGGAATTAACCTCAGAATGGAAGAAAAAATTCTTCTTTCCTTTTTTTGTTGAACTGAACATGTTTACTAATGACCAATTTTTTCTCAAAAAATTTACAGCAATCCAAACGAATAACTTGGGATCACCAATGACTTTGGATACGGGATCAGTAGCCACACCTTATGATTATGGGGCAGGAGAAATATCAACCAATGGAGCATTACAACCAGGACTAGTCTATGAAACTAGTACAACAGACTACTTACTATACCTTTGCGGCCGAGGTTATGATCTAACCACCATTAAGAGCATCACAACCACTGTTCCTGATGGATTTGATTGCCCCAAGAATTCAACTGCAGACTACATATCCAACATGAACTACCCAACAATAGCAGTATCTGAATTGAAAGGCAAGGAAAGCAAGAAAGTAATCAGAACAGTTACAAATGTTGGTGGCAATGGTGAAACAGTCTACACAGTCAGTGTAGACGCACCTGAGGAAGTAGAGGTCAAAGTGATTCCAGAGAAATTGAAATTTGCAAAGAACAATGAGAAGCAGAGTTATCAAGTGGTTTTCACCCCAACTGTATCCACACTGAAGAAAGGAGGCTTTGGTTCAATCACTTGGACCAATGGAAAACATCGAGTCCGAAGTCCATTTGTCGTCACTAGTCACTAGTGAGAGTAGTGAGCCATAAGATTACTTATAAAACAGTGAACACATTGCAATTAACCAGGTCAAAAGTTTGTAATTCTCTAGTCATCTGTTTCAGTTTGGACTATGGTACTTTCTGAATAAACGAAGTTGGTGTTTTGGATGTC

mRNA sequence

CTCCACCATGGCCACTGCTGTTATTTCAGTCCGCCACCATTTGAATTACCATGGCGCACTACGTGTGGATAAGGGAAAAGCTTGGCATTTTGGCAGTGCGCGTGCTCTGATCATGAAACCCACCAACTGATCATCTCTCATTGAATTTTTCCCCATATTCCTTCTTCACCACCAGTTTTCATCTGTAAAAACATATAAATTAGCGCTTGAAGTCGAAGGGAACTTACTCAATTAGATCAATACAGAAGCCAATTAGCCAAGCCAATACGCCATGCAAAGCATTTCTTTTTGTGTCTTTTTCGCTTTGGTTTGTGTCATCTTTTTTCTTGTTTCTGAAAATGTTAAAGTAGCTGATGCTGCTGAAGATGCAAAAAATGGTGTTTATGTCGTTTACATGGGATCCGCTTCTGGCTCCAGGACTGACTTCCTTCGCCTTCTTAACTCTGTCAACAGACGGAACGCAGTGGTACACACATATAAACATGGGTTCACAGGATTTGCAGCTCGTCTATCAGAACATGAAGCCCAAGCCATGAGACAAAACCCTGGAGTTGTTTCGGTTTTTCCAGATCCAATATTGAAGTTACACACAACTCATTCATGGGATTTCTTGGTCAGTCAGACAAGCGTCAAAATCGACGCCAATCCCAAATCAGATCCCCCAACCTCTTCCTCTCAACCATACGATACCATTATAGGAATCTTGGACACTGGTATCTGGCCGGAATCGGAGAGTTTTAATGACGAGGGTATGGGTCCGATACCGTCGAGGTGGAAAGGTACCTGCATGGTAGGCGATGACTTCACTTCCTCCAACTGTAATAGAAAAATCATTGGAGCAAGATTTTACGAAAGTTCTGAGAGCGACGGCATACGGTTCCACTCACCTCGAGACGGAGCTGGACATGGCACCCACGTGGCATCAACGGCGGCCGGAAGTGCCGTGGCTAATGCATCGTACTATGGCCTCGCAGCCGGAACGGCCAAGGGCGGCTCCCCCGGCTCAAGGATCGCCATGTACAGAGTCTGCATGGCCGACGGCTGTCGTGGATCGTCAATTCTGAAGGCGTTTGATGATTCCATAGCCGATGGGGTCGATGTATTGTCCTTATCTCTCGGGACACCCTCCATATTCCGACAAAATATGACGGTCGACCCTATTGCTATCGGAGCCTTTCATGCGGTTGAGAAGGGCATTACAGTTGTCTGCTCCGCCGGAAACGACGGTCCCAGTTATGGAACGGTGGTGAACGACGCGCCGTGGATTCTAACAGTGGCCGCCTCCACCATTGATAGGGATTTTGAGTCTGATGTTGTGTTGGGCAACAAAAAAGTGATCAAGGGCGAAGGTATAAATTTCTCTGATCTTCAAAAGTCTCCTGTATACCCACTGATACAAGGCAAGTCAGCCAAGAAAGCGAGTGCCAGTGAAGACAGTGCAAGGATTTGTTCTGAAGACTCCATGGATGAAGCTCAAGTGAAGAGGAAGATAGTTATTTGCGAAAGCAATGTCGAAGGAGGCGGTTCGGATTGGCAAAGTCAGGCTGAAGCAGTGAAGAATCTTGGAGGGGTTGGGGTAGTTTTGATTGATGACGACTCAAAATTAGTTGCAGAGAAGTTTCCTACTCCCATGACAGTTATTAGCAAAAAGGATGGTCCTGAGATCCTCTCCTACGTTAACTCAAGCAGGAAACCAGTTGCTACAATTCTCCCCACTGAGACCATAATAAACTATAAGCCAGCACCTGCTATAACATATTTTTCATCCAGAGGGCCTAATCCCGAAGTATTAAACATAATCAAGCCGGACATATCAGCACCAGGAGTGAACATTCTTGCAGCCTGGCTTGGCAATGATTCAAGCCCAACCCCACAAGCAACAAAGTCACCACTCTTCAATGTGATCTCAGGAACTTCAATGTCCTGCCCCCATGTCTCTGGCGTAGTGGCCTCTGTAAAATCTCAAAACCCCACATGGAGTCCCTCAGCAATCAAATCAGCTATCATGACAACAGCAATCCAAACGAATAACTTGGGATCACCAATGACTTTGGATACGGGATCAGTAGCCACACCTTATGATTATGGGGCAGGAGAAATATCAACCAATGGAGCATTACAACCAGGACTAGTCTATGAAACTAGTACAACAGACTACTTACTATACCTTTGCGGCCGAGGTTATGATCTAACCACCATTAAGAGCATCACAACCACTGTTCCTGATGGATTTGATTGCCCCAAGAATTCAACTGCAGACTACATATCCAACATGAACTACCCAACAATAGCAGTATCTGAATTGAAAGGCAAGGAAAGCAAGAAAGTAATCAGAACAGTTACAAATGTTGGTGGCAATGGTGAAACAGTCTACACAGTCAGTGTAGACGCACCTGAGGAAGTAGAGGTCAAAGTGATTCCAGAGAAATTGAAATTTGCAAAGAACAATGAGAAGCAGAGTTATCAAGTGGTTTTCACCCCAACTGTATCCACACTGAAGAAAGGAGGCTTTGGTTCAATCACTTGGACCAATGGAAAACATCGAGTCCGAAGTCCATTTGTCGTCACTAGTCACTAGTGAGAGTAGTGAGCCATAAGATTACTTATAAAACAGTGAACACATTGCAATTAACCAGGTCAAAAGTTTGTAATTCTCTAGTCATCTGTTTCAGTTTGGACTATGGTACTTTCTGAATAAACGAAGTTGGTGTTTTGGATGTC

Coding sequence (CDS)

ATGCAAAGCATTTCTTTTTGTGTCTTTTTCGCTTTGGTTTGTGTCATCTTTTTTCTTGTTTCTGAAAATGTTAAAGTAGCTGATGCTGCTGAAGATGCAAAAAATGGTGTTTATGTCGTTTACATGGGATCCGCTTCTGGCTCCAGGACTGACTTCCTTCGCCTTCTTAACTCTGTCAACAGACGGAACGCAGTGGTACACACATATAAACATGGGTTCACAGGATTTGCAGCTCGTCTATCAGAACATGAAGCCCAAGCCATGAGACAAAACCCTGGAGTTGTTTCGGTTTTTCCAGATCCAATATTGAAGTTACACACAACTCATTCATGGGATTTCTTGGTCAGTCAGACAAGCGTCAAAATCGACGCCAATCCCAAATCAGATCCCCCAACCTCTTCCTCTCAACCATACGATACCATTATAGGAATCTTGGACACTGGTATCTGGCCGGAATCGGAGAGTTTTAATGACGAGGGTATGGGTCCGATACCGTCGAGGTGGAAAGGTACCTGCATGGTAGGCGATGACTTCACTTCCTCCAACTGTAATAGAAAAATCATTGGAGCAAGATTTTACGAAAGTTCTGAGAGCGACGGCATACGGTTCCACTCACCTCGAGACGGAGCTGGACATGGCACCCACGTGGCATCAACGGCGGCCGGAAGTGCCGTGGCTAATGCATCGTACTATGGCCTCGCAGCCGGAACGGCCAAGGGCGGCTCCCCCGGCTCAAGGATCGCCATGTACAGAGTCTGCATGGCCGACGGCTGTCGTGGATCGTCAATTCTGAAGGCGTTTGATGATTCCATAGCCGATGGGGTCGATGTATTGTCCTTATCTCTCGGGACACCCTCCATATTCCGACAAAATATGACGGTCGACCCTATTGCTATCGGAGCCTTTCATGCGGTTGAGAAGGGCATTACAGTTGTCTGCTCCGCCGGAAACGACGGTCCCAGTTATGGAACGGTGGTGAACGACGCGCCGTGGATTCTAACAGTGGCCGCCTCCACCATTGATAGGGATTTTGAGTCTGATGTTGTGTTGGGCAACAAAAAAGTGATCAAGGGCGAAGGTATAAATTTCTCTGATCTTCAAAAGTCTCCTGTATACCCACTGATACAAGGCAAGTCAGCCAAGAAAGCGAGTGCCAGTGAAGACAGTGCAAGGATTTGTTCTGAAGACTCCATGGATGAAGCTCAAGTGAAGAGGAAGATAGTTATTTGCGAAAGCAATGTCGAAGGAGGCGGTTCGGATTGGCAAAGTCAGGCTGAAGCAGTGAAGAATCTTGGAGGGGTTGGGGTAGTTTTGATTGATGACGACTCAAAATTAGTTGCAGAGAAGTTTCCTACTCCCATGACAGTTATTAGCAAAAAGGATGGTCCTGAGATCCTCTCCTACGTTAACTCAAGCAGGAAACCAGTTGCTACAATTCTCCCCACTGAGACCATAATAAACTATAAGCCAGCACCTGCTATAACATATTTTTCATCCAGAGGGCCTAATCCCGAAGTATTAAACATAATCAAGCCGGACATATCAGCACCAGGAGTGAACATTCTTGCAGCCTGGCTTGGCAATGATTCAAGCCCAACCCCACAAGCAACAAAGTCACCACTCTTCAATGTGATCTCAGGAACTTCAATGTCCTGCCCCCATGTCTCTGGCGTAGTGGCCTCTGTAAAATCTCAAAACCCCACATGGAGTCCCTCAGCAATCAAATCAGCTATCATGACAACAGCAATCCAAACGAATAACTTGGGATCACCAATGACTTTGGATACGGGATCAGTAGCCACACCTTATGATTATGGGGCAGGAGAAATATCAACCAATGGAGCATTACAACCAGGACTAGTCTATGAAACTAGTACAACAGACTACTTACTATACCTTTGCGGCCGAGGTTATGATCTAACCACCATTAAGAGCATCACAACCACTGTTCCTGATGGATTTGATTGCCCCAAGAATTCAACTGCAGACTACATATCCAACATGAACTACCCAACAATAGCAGTATCTGAATTGAAAGGCAAGGAAAGCAAGAAAGTAATCAGAACAGTTACAAATGTTGGTGGCAATGGTGAAACAGTCTACACAGTCAGTGTAGACGCACCTGAGGAAGTAGAGGTCAAAGTGATTCCAGAGAAATTGAAATTTGCAAAGAACAATGAGAAGCAGAGTTATCAAGTGGTTTTCACCCCAACTGTATCCACACTGAAGAAAGGAGGCTTTGGTTCAATCACTTGGACCAATGGAAAACATCGAGTCCGAAGTCCATTTGTCGTCACTAGTCACTAG

Protein sequence

MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDFLRLLNSVNRRNAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSVKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFTSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIAIGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGGSDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVATILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATKSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGSVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPKNSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPEKLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTSH
Homology
BLAST of PI0012342 vs. ExPASy Swiss-Prot
Match: Q9LNU1 (CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 1.1e-253
Identity = 459/768 (59.77%), Postives = 574/768 (74.74%), Query Frame = 0

Query: 6   FCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSAS----GSRTDFLRLLNSVNR 65
           F  F  L+C++F   +E      A     +GVY+VYMGSAS     +R   L       R
Sbjct: 10  FLSFLYLLCILFMTETE------AGSRNGDGVYIVYMGSASSAANANRAQILINTMFKRR 69

Query: 66  RNAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSVK 125
            N ++HTYKHGF+GFAARL+  EA+ + + PGVVSVFPDP  +LHTTHSWDFL  QTSVK
Sbjct: 70  ANDLLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVK 129

Query: 126 IDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFTSS 185
           +D+ P   P ++S   YD+I+GILDTGIWPESESFND+ MGPIPSRWKGTCM   DF SS
Sbjct: 130 VDSGP---PSSASDGSYDSIVGILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKSS 189

Query: 186 NCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGG 245
           NCNRKIIGAR+Y++ + D   +++ RD  GHG+HV+ST AGSAV NASYYG+A+GTAKGG
Sbjct: 190 NCNRKIIGARYYKNPDDDS-EYYTTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGG 249

Query: 246 SPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIAIGA 305
           S  +RIAMY+VC   GC GSSIL AFDD+IADGVDVLSLSLG P+  R ++  DPIAIGA
Sbjct: 250 SQNARIAMYKVCNPGGCTGSSILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGA 309

Query: 306 FHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGI 365
           FHAVE+GI V+CSAGNDGP  GTV N APWI+TVAA+TIDRDFESDVVLG  KVIKGEGI
Sbjct: 310 FHAVEQGILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGI 369

Query: 366 NFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGGSDW 425
           +FS++ KSPVYPLI GKSAK A ASE SAR C  DS+D+ +VK KIV+CE NV  GGS +
Sbjct: 370 HFSNVSKSPVYPLIHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCE-NV--GGSYY 429

Query: 426 QSQA-EAVKNLGGVGVVLIDDDSKLVAEKFPT-PMTVISKKDGPEILSYVNSSRKPVATI 485
            S A + VK+ GG G V +DD ++ VA  + + P TVI  K+  EI SY+NS++ PVATI
Sbjct: 430 ASSARDEVKSKGGTGCVFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATI 489

Query: 486 LPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATKS 545
           LPT T+  + PAPA+ YFSSRGP+    +I+KPDI+APGV+ILAAW GNDSS + +   +
Sbjct: 490 LPTATVEKFTPAPAVAYFSSRGPSSLTRSILKPDITAPGVSILAAWTGNDSSISLEGKPA 549

Query: 546 PLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGSV 605
             +NVISGTSM+ PHVS V + +KSQ+PTW PSAI+SAIMTTA QTNN    +T +TG+ 
Sbjct: 550 SQYNVISGTSMAAPHVSAVASLIKSQHPTWGPSAIRSAIMTTATQTNNDKGLITTETGAT 609

Query: 606 ATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPKN 665
           ATPYD GAGE+S+  ++QPGLVYET+ TDYL +LC  GY++TTIK+++   P+ F CP +
Sbjct: 610 ATPYDSGAGELSSTASMQPGLVYETTETDYLNFLCYYGYNVTTIKAMSKAFPENFTCPAD 669

Query: 666 STADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPEK 725
           S  D IS +NYP+I +S  KG  SK V RTVTNVG +GE VYTVSV+ P    ++V PEK
Sbjct: 670 SNLDLISTINYPSIGISGFKGNGSKTVTRTVTNVGEDGEAVYTVSVETPPGFNIQVTPEK 729

Query: 726 LKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           L+F K+ EK +YQV+ + T S LK+  FG++TW+N K++VRSP V++S
Sbjct: 730 LQFTKDGEKLTYQVIVSATAS-LKQDVFGALTWSNAKYKVRSPIVISS 763

BLAST of PI0012342 vs. ExPASy Swiss-Prot
Match: F4HSQ2 (Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 5.6e-221
Identity = 410/777 (52.77%), Postives = 552/777 (71.04%), Query Frame = 0

Query: 7   CVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSAS---GSRTDFLRLLNSVNRRN 66
           C+   ++  +FF +S   K    +E +K+G Y++YMG+AS    +  D + LL+S+ +R+
Sbjct: 4   CLTITIMFFMFFFLSVIQKC--KSETSKSGDYIIYMGAASSDGSTDNDHVELLSSLLQRS 63

Query: 67  --AVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSVK 126
               +H YKHGF+GFAA LSE EA  + + PGV+SVFPD +L+LHTT SWDFLV Q S +
Sbjct: 64  GKTPMHRYKHGFSGFAAHLSEDEAHLIAKQPGVLSVFPDQMLQLHTTRSWDFLV-QESYQ 123

Query: 127 IDA--NPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDF- 186
            D      +    S     DTIIG LD+GIWPE++SFND  MGP+P +WKGTCM G    
Sbjct: 124 RDTYFTEMNYEQESEMHEGDTIIGFLDSGIWPEAQSFNDRHMGPVPEKWKGTCMRGKKTQ 183

Query: 187 -TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGT 246
             S  CNRK+IGAR+Y SS      + +PRD  GHGTHVAS AAG  +ANASYYGLA+G 
Sbjct: 184 PDSFRCNRKLIGARYYNSSFFLDPDYETPRDFLGHGTHVASIAAGQIIANASYYGLASGI 243

Query: 247 AKGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPI 306
            +GGSP SRIAMYR C   GCRGSSIL AFDD+IADGVDV+S+S+G   ++  N+  DP+
Sbjct: 244 MRGGSPSSRIAMYRACSLLGCRGSSILAAFDDAIADGVDVISISMG---LWPDNLLEDPL 303

Query: 307 AIGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLG--NKKV 366
           +IG+FHAVE+GITVVCS GN GPS  +V N APW++TVAASTIDR FES+++LG    ++
Sbjct: 304 SIGSFHAVERGITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDENRL 363

Query: 367 IKGEGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVE 426
           I+G GIN +++ K+  YPLI  +SAKK  A+E++AR C+ D++D+  VK KIV+C+S+++
Sbjct: 364 IEGFGINIANIDKTQAYPLIHARSAKKIDANEEAARNCAPDTLDQTIVKGKIVVCDSDLD 423

Query: 427 GGGSDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPT-PMTVISKKDGPEILSYVNSSRK 486
                W+S  + VK LGG+G+VL+DD+S  ++   P+  +T+I  +DG +I+SY+NS+R+
Sbjct: 424 NQVIQWKS--DEVKRLGGIGMVLVDDESMDLSFIDPSFLVTIIKPEDGIQIMSYINSTRE 483

Query: 487 PVATILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTP 546
           P+ATI+PT +   +  AP+I  FSSRGP     +I+KPDI+APGVNILA+WL  D +  P
Sbjct: 484 PIATIMPTRSRTGHMLAPSIPSFSSRGPYLLTRSILKPDIAAPGVNILASWLVGDRNAAP 543

Query: 547 QATKSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTL 606
           +    PLFN+ SGTSMSCPHVSG+ A +KS+ P+WSP+AI+SAIMTTA+Q  N GS +T 
Sbjct: 544 EGKPPPLFNIESGTSMSCPHVSGIAARLKSRYPSWSPAAIRSAIMTTAVQMTNTGSHITT 603

Query: 607 DTGSVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGF 666
           +TG  ATPYD+GAG+++  G   PGL+YET+  DYL +L   G+    IK I+  +P GF
Sbjct: 604 ETGEKATPYDFGAGQVTIFGPSSPGLIYETNHMDYLNFLGYYGFTSDQIKKISNRIPQGF 663

Query: 667 DCPKNSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVG----GNGETVYTVSVDAPEE 726
            CP+ S    ISN+NYP+I++S   GKES++V RTVTNV     G+ +TVYTVS+DAPE 
Sbjct: 664 ACPEQSNRGDISNINYPSISISNFNGKESRRVSRTVTNVASRLIGDEDTVYTVSIDAPEG 723

Query: 727 VEVKVIPEKLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           + V+VIP +L F K  +K SYQV+F+ T + LK   FGSITW+NG + VRSPFVVTS
Sbjct: 724 LLVRVIPRRLHFRKIGDKLSYQVIFSSTTTILKDDAFGSITWSNGMYNVRSPFVVTS 772

BLAST of PI0012342 vs. ExPASy Swiss-Prot
Match: A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 2.7e-159
Identity = 332/746 (44.50%), Postives = 456/746 (61.13%), Query Frame = 0

Query: 38  YVVYMGSASGSRTDFL-----RLLNSV-----NRRNAVVHTYKHGFTGFAARLSEHEAQA 97
           Y+VYMG  S   ++ +      +L SV     + + A +H Y   F GF+A ++  +A+ 
Sbjct: 28  YIVYMGDRSHPNSESVVRANHEILASVTGSLNDAKAAAIHHYSRSFQGFSAMITPEQAKK 87

Query: 98  MRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSVKIDANPKSDPPTSSSQPYDTIIGILDT 157
           +  +  VVSVF   + KLHTTHSWDFL   T  K       + P++     + I+G++D+
Sbjct: 88  LADHNSVVSVFESKMNKLHTTHSWDFLGLDTVYK-------NNPSALDSASNVIVGVIDS 147

Query: 158 GIWPESESFNDEGMGPIPSRWKGTCMVGDDFTSSNCNRKIIGARFYESSES--------- 217
           G+WPESESFND G+GP+P ++KG C+ GD+FT +NCN+KIIGARFY              
Sbjct: 148 GVWPESESFNDYGLGPVPEKFKGECVTGDNFTLANCNKKIIGARFYSKGLEAEIGPLENI 207

Query: 218 -DGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADG 277
            D I F SPRD  GHGTH AST AGS V+N S +G+A GTA+GG+P +R+++Y+ C    
Sbjct: 208 VDSIFFRSPRDSDGHGTHTASTIAGSIVSNVSLFGMAKGTARGGAPSARLSIYKACWFGF 267

Query: 278 CRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIAIGAFHAVEKGITVVCSAGN 337
           C  + +  A DD+I DGVD+LSLSLG P   +     + I++GAFHA +KGI V  SAGN
Sbjct: 268 CSDADVFAAMDDAIHDGVDILSLSLG-PDPPQPLYFENAISVGAFHAFQKGILVSASAGN 327

Query: 338 DGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGINFSDLQKSPVYPLIQG 397
                 T  N APWI TVAAST+DR+F SD+ LGN KV+KG  +N   ++ S  Y LI G
Sbjct: 328 -SVFPRTACNVAPWIFTVAASTVDREFRSDIYLGNSKVLKGLSLNPIKMEGS--YGLIYG 387

Query: 398 KSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGGSDWQSQAEAVKNLGGVGVV 457
            +A  A  +  +A  C E ++D   +K KIVIC   VE    + + +A  +K  GGVG++
Sbjct: 388 SAAAAAGDAALNASFCKEHTLDPTLIKGKIVIC--TVEKFTDNRREKAIIIKQGGGVGMI 447

Query: 458 LIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVATILPTETIINYKPAPAITYF 517
           LID +++ V  +F  P T+I +    E+ +Y+ + + P ATI PT T++  KPAP    F
Sbjct: 448 LIDHNARDVGFQFVIPSTMIGQDAVEELQAYMKTEKNPTATIFPTLTLVGTKPAPESAAF 507

Query: 518 SSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATKSPLFNVISGTSMSCPHVSG 577
           SS GPN    +IIKPDI+ PGVNILAAW    +  T +  KS  +N+ISGTSMSCPH+S 
Sbjct: 508 SSVGPNIITPDIIKPDITGPGVNILAAWSPVATEATVE-QKSVNYNIISGTSMSCPHISA 567

Query: 578 VVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLD-TGSVATPYDYGAGEISTNGAL 637
           + A +KS +P+WSP+AI SAIMT+A   +N  S +  D  G+ ATP+DYG+G ++   +L
Sbjct: 568 ISAIIKSHHPSWSPAAIMSAIMTSATVMDNTHSLIGRDPNGTQATPFDYGSGHVNPVASL 627

Query: 638 QPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPKNSTADYISNMNYPTIAVS 697
            PGLVY+ S+ D L +LC  G     +K++T  +     C K+ TA Y  N NYP+I VS
Sbjct: 628 NPGLVYDFSSQDVLNFLCSNGASPAQLKNLTGELT---QCQKSPTASY--NFNYPSIGVS 687

Query: 698 ELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPEKLKFAKNNEKQSYQVVFT 757
            L G  S  V RTVT   G   T Y  SV+ P  V V+V P KLKF K  EK ++++ FT
Sbjct: 688 NLNG--SLSVYRTVTYY-GQEPTEYFASVERPSGVIVRVTPAKLKFWKAGEKITFRIDFT 747

Query: 758 PTVSTLKKGGFGSITWTNGKHRVRSP 763
           P  ++     FG++TW NGK RVRSP
Sbjct: 748 PFKNSNGNFVFGALTWNNGKQRVRSP 751

BLAST of PI0012342 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 4.1e-147
Identity = 333/773 (43.08%), Postives = 449/773 (58.09%), Query Frame = 0

Query: 11  ALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDF------LRLLNSVNRRNA 70
           A  C+  +L+   +    A  D    VY+VYMGS S SR D+      + +L  V   ++
Sbjct: 6   ASTCLYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLS-SRADYIPTSDHMSILQQVTGESS 65

Query: 71  V----VHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSV 130
           +    V +YK  F GFAARL+E E   + +  GVVSVFP+ IL+LHTT SWDF+     V
Sbjct: 66  IEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFM----GV 125

Query: 131 KIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFTS 190
           K   N K +    S    DTIIG++DTGIWPES+SF+D+G GP P +WKG C  G +FT 
Sbjct: 126 KEGKNTKRNLAIES----DTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT- 185

Query: 191 SNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKG 250
             CN K+IGAR Y S           RD +GHGTH ASTAAG+AV + S++G+  GT +G
Sbjct: 186 --CNNKLIGARDYTS--------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 245

Query: 251 GSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLG--TPSIFRQNMTVDPIA 310
           G P SRIA Y+VC   GC   ++L +FDD+IADGVD++++S+G   PSIF      DPIA
Sbjct: 246 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFED----DPIA 305

Query: 311 IGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 370
           IGAFHA+ KGI  V SAGN GP   TV + APWI TVAAST +R F + VVLGN K + G
Sbjct: 306 IGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAG 365

Query: 371 EGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGG 430
             +N  D+ K   YPL+ GKSA  ++    +A +C+   +++++VK KI++C     GG 
Sbjct: 366 RSVNAFDM-KGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVC-----GGP 425

Query: 431 SDWQSQAEAVKNLGGVGVVLIDDDSKL--VAEKFPTPMTVISKKDGPEILSYVNSSRKPV 490
           S +       K    VG + I D S    VA     P + +  KD   ++SY+ S   P 
Sbjct: 426 SGY-------KIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQ 485

Query: 491 ATILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQA 550
           A +L TETI N + +P I  FSSRGPN   ++I+KPDI+APGV ILAA+  N   P+   
Sbjct: 486 AAVLKTETIFN-RTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPN-GEPSEDD 545

Query: 551 TKSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDT 610
           T+   ++V SGTSM+CPHV+GV A VK+  P WSPS I+SAIMTTA      G       
Sbjct: 546 TRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKG------R 605

Query: 611 GSVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDC 670
           G  +T + YGAG +    AL PGLVYE    D++ +LCG  Y   T+K I+    D   C
Sbjct: 606 GIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIIS---GDTVKC 665

Query: 671 PKNSTADYISNMNYPTIAVSELKGKESKKVI---RTVTNVGGNGETVYT-VSVDAPEEVE 730
            K +      N+NYP+++ ++L G +S   +   RT+TNVG    T  + V      ++ 
Sbjct: 666 SKKNKI-LPRNLNYPSMS-AKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLS 725

Query: 731 VKVIPEKLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVV 766
           +KV P  L F   NEKQS+ V  T +    +     ++ W++G H VRSP VV
Sbjct: 726 IKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVV 728

BLAST of PI0012342 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 2.7e-146
Identity = 317/767 (41.33%), Postives = 453/767 (59.06%), Query Frame = 0

Query: 15  VIFFLVSENV-KVADAAEDAKNGVYVVYMGSASGSRTDFLRLLNSVNRRNAVVHTYKHGF 74
           V++F    +V ++ + A D     +  ++GS +GSR             +A+ ++Y    
Sbjct: 33  VVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE---------RATDAIFYSYTKHI 92

Query: 75  TGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSVKIDANPKSDPPTS 134
            GFAA L    A  + ++P VVSVFP+  LKLHTT SWDFL  + +  +   P S     
Sbjct: 93  NGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYV---PSSSIWRK 152

Query: 135 SSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFTSSNCNRKIIGARFY 194
           +    DTII  LDTG+WPES+SF DEG+GPIPSRWKG C    D T  +CNRK+IGAR++
Sbjct: 153 ARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT-FHCNRKLIGARYF 212

Query: 195 ESSESDGI-----RFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGGSPGSRIA 254
               +  +      F SPRD  GHG+H  STAAG  V   S +G   GTAKGGSP +R+A
Sbjct: 213 NKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVA 272

Query: 255 MYRVC----MADGCRGSSILKAFDDSIADGVDVLSLSL-GTPSIFRQNMTVDPIAIGAFH 314
            Y+VC      + C  + +L AFD +I DG DV+S+SL G P+ F      D +AIG+FH
Sbjct: 273 AYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFN----DSVAIGSFH 332

Query: 315 AVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGINF 374
           A +K I VVCSAGN GP+  TV N APW +TV AST+DR+F S++VLGN K  KG+ ++ 
Sbjct: 333 AAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSS 392

Query: 375 SDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGGSDWQS 434
           + L  +  YP++   +AK  +AS   A++C   S+D  + K KI++C     G       
Sbjct: 393 TALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGR----VE 452

Query: 435 QAEAVKNLGGVGVVLID---DDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVATIL 494
           +  AV   GG+G+VL +     + L+A+    P T ++ KD   +  Y++ ++KP+A I 
Sbjct: 453 KGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHIT 512

Query: 495 PTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQ-ATKS 554
           P+ T +  KPAP +  FSS+GP+     I+KPDI+APGV+++AA+ G  S    Q   + 
Sbjct: 513 PSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRR 572

Query: 555 PLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGSV 614
            LFN ISGTSMSCPH+SG+   +K++ P+WSP+AI+SAIMTTA   +++  P+   T   
Sbjct: 573 LLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMK 632

Query: 615 ATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPKN 674
           ATP+ +GAG +  N A+ PGLVY+    DYL +LC  GY+ + I   +    + F C  +
Sbjct: 633 ATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFS---GNNFTC--S 692

Query: 675 STADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPEK 734
           S    + N+NYP+I V  L   +   V RTV NVG    ++YTV V+ P+ V V V P  
Sbjct: 693 SPKISLVNLNYPSITVPNLTSSK-VTVSRTVKNVG--RPSMYTVKVNNPQGVYVAVKPTS 752

Query: 735 LKFAKNNEKQSYQVVFTPTVSTLKKG-GFGSITWTNGKHRVRSPFVV 766
           L F K  E+++++V+   +   + KG  FG + W++ KHRVRSP VV
Sbjct: 753 LNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVV 770

BLAST of PI0012342 vs. ExPASy TrEMBL
Match: A0A0A0LJ71 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G873800 PE=3 SV=1)

HSP 1 Score: 1434.1 bits (3711), Expect = 0.0e+00
Identity = 728/768 (94.79%), Postives = 748/768 (97.40%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSA-SGSRTDFLRLLNSV 60
           MQSISFCVFFALVCV FFLVSENVKVADAAEDA+NGVY+VYMGSA SG RTDFLRLLNSV
Sbjct: 1   MQSISFCVFFALVCVTFFLVSENVKVADAAEDARNGVYIVYMGSASSGFRTDFLRLLNSV 60

Query: 61  NRRNAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTS 120
           NRRNAVVHTYKHGFTGFAA LSEHEAQAMRQ+PGVVSVFPDP+LKLHTTHSWDFLVSQTS
Sbjct: 61  NRRNAVVHTYKHGFTGFAAHLSEHEAQAMRQSPGVVSVFPDPLLKLHTTHSWDFLVSQTS 120

Query: 121 VKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFT 180
           VKIDANPKSDPP SSSQPYDTIIGILDTGIWPESESFND GMGPIPSRWKGTCM GDDFT
Sbjct: 121 VKIDANPKSDPPASSSQPYDTIIGILDTGIWPESESFNDMGMGPIPSRWKGTCMTGDDFT 180

Query: 181 SSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240
           SSNCNRKIIGARFYESSESDGIR+HSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK
Sbjct: 181 SSNCNRKIIGARFYESSESDGIRYHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240

Query: 241 GGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIAI 300
           GGSPGSRIAMYRVCMADGCRGSSI+KAFDDSIADGVDVLSLSLGTPS+FR ++T DPIAI
Sbjct: 241 GGSPGSRIAMYRVCMADGCRGSSIMKAFDDSIADGVDVLSLSLGTPSVFRPDLTADPIAI 300

Query: 301 GAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360
           GAFHAVEKGITVVCSAGNDGPS GTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE
Sbjct: 301 GAFHAVEKGITVVCSAGNDGPSSGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360

Query: 361 GINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGGS 420
           GINFSDLQKSPVYPLI+GKSAKKAS SEDSARICSEDSMDEAQVK KIVICE++VEGGGS
Sbjct: 361 GINFSDLQKSPVYPLIEGKSAKKASDSEDSARICSEDSMDEAQVKGKIVICENSVEGGGS 420

Query: 421 DWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVATI 480
           DWQSQAE VKNLGGVG+VLIDDDSKLVAEKF TPMTVISKKDG EILSYVNSSRKPVAT+
Sbjct: 421 DWQSQAETVKNLGGVGLVLIDDDSKLVAEKFSTPMTVISKKDGLEILSYVNSSRKPVATV 480

Query: 481 LPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATKS 540
           LPTETIINYKPAPAITYFSSRGPNP VLNIIKPDISAPGVNILAAWLGNDSS TPQATKS
Sbjct: 481 LPTETIINYKPAPAITYFSSRGPNPAVLNIIKPDISAPGVNILAAWLGNDSSSTPQATKS 540

Query: 541 PLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGSV 600
           PLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAI+SAIMTTAIQTNNLGSPMTLDTGSV
Sbjct: 541 PLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIRSAIMTTAIQTNNLGSPMTLDTGSV 600

Query: 601 ATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPKN 660
           ATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGY+LTTIKSITTT+PDGFDCPKN
Sbjct: 601 ATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYNLTTIKSITTTIPDGFDCPKN 660

Query: 661 STADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPEK 720
           S ADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAP+EVEVKVIPEK
Sbjct: 661 SNADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPQEVEVKVIPEK 720

Query: 721 LKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           LKFAKN EKQSYQVVFTPTVST+K+ GFGSITWTNGKHRVRSPFVVTS
Sbjct: 721 LKFAKNYEKQSYQVVFTPTVSTMKR-GFGSITWTNGKHRVRSPFVVTS 767

BLAST of PI0012342 vs. ExPASy TrEMBL
Match: A0A6J1FFB5 (CO(2)-response secreted protease OS=Cucurbita moschata OX=3662 GN=LOC111443427 PE=3 SV=1)

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 654/769 (85.05%), Postives = 698/769 (90.77%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDFLRLLNSVN 60
           MQ+IS CVF  LVCVIF L+SEN K+A AA    NGVY+VYMGSA  SRTDFLRLL SVN
Sbjct: 1   MQTISSCVFL-LVCVIFLLLSENAKIA-AAAAGNNGVYIVYMGSA--SRTDFLRLLTSVN 60

Query: 61  RR--NAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQT 120
           RR  NAVV TYKHGFTGFAARLSE EA AMRQ PGVVSVFPDPILKLHTTHSWDFLVSQT
Sbjct: 61  RRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQT 120

Query: 121 SVKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDF 180
           SVKID+ PK+DPPTSSSQP+DTIIGILDTGIWPESESF DEGM PIPSRWKGTCM GDDF
Sbjct: 121 SVKIDSAPKADPPTSSSQPFDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 180

Query: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240
           TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA
Sbjct: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240

Query: 241 KGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIA 300
           KGGSPGSRIAMYRVCMADGC GSSIL AFDD+I DGVDVLSLSLG P+ ++  +T DPIA
Sbjct: 241 KGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPNYYKPELTTDPIA 300

Query: 301 IGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360
           IGAFHAVEKGITVVCSAGNDGP+ G+VVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG
Sbjct: 301 IGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360

Query: 361 EGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGG 420
           EGINFS L+ SPVYPLI GKSA K+S SEDSARICSE SMDEA VK KIVICES+ EGGG
Sbjct: 361 EGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGG 420

Query: 421 SDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVAT 480
           S WQ QAE V++LGGVG+VLIDD +KLVAEKF +P+T IS KDG E+L+YV+SSR PVAT
Sbjct: 421 SYWQGQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVAT 480

Query: 481 ILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATK 540
           ILPT T+INYKPAPAI YFSSRGPNP VLN+IKPDISAPGVNILAAWLGND++ TPQ   
Sbjct: 481 ILPTLTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540

Query: 541 SPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGS 600
           SPLFNVISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTTAIQTNNL SPMTLDTGS
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGS 600

Query: 601 VATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPK 660
           VATPYDYGAGEIST GAL+PGLVYETSTTDYL+YLCGRGY+ +TIKSI  TVPDGFDCPK
Sbjct: 601 VATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPK 660

Query: 661 NSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPE 720
           +S ADYISNMNYPTIAVSELKG ESKK++RTVTNVGG+G TVY VSVDAP EV+VKVIPE
Sbjct: 661 DSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPE 720

Query: 721 KLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           KL+F+KNN+KQSY+VVFT TVSTLKK  FGSITW+NGKHRVRSPFVVTS
Sbjct: 721 KLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTS 765

BLAST of PI0012342 vs. ExPASy TrEMBL
Match: A0A6J1IHT1 (CO(2)-response secreted protease OS=Cucurbita maxima OX=3661 GN=LOC111476411 PE=3 SV=1)

HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 652/769 (84.79%), Postives = 695/769 (90.38%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDFLRLLNSVN 60
           MQ+IS C F  LVCVIF L+SEN K+A AA    NGVY+VYMGSA  SRTDFLRLL SVN
Sbjct: 1   MQTISSCAFL-LVCVIFLLLSENAKIA-AAAAGNNGVYIVYMGSA--SRTDFLRLLTSVN 60

Query: 61  RR--NAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQT 120
           RR  NAVV TYKHGFTGFAARLSE EA AMRQ PGVVSVFPDPILKLHTTHSWDFLVSQT
Sbjct: 61  RRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQT 120

Query: 121 SVKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDF 180
           SVKID+ PKS+PPTSSSQP DTIIGILDTGIWPESESF DEGM PIPSRWKGTCM GDDF
Sbjct: 121 SVKIDSAPKSEPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 180

Query: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240
           TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA
Sbjct: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240

Query: 241 KGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIA 300
           KGGSPGSRIAMYRVCMADGC GSSIL AFDD+I DGVDVLSLSLGTP  ++  +  DPIA
Sbjct: 241 KGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGTPYFYKPELATDPIA 300

Query: 301 IGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360
           IGAFHAVEKGITVVCSAGNDGP+ G+VVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG
Sbjct: 301 IGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360

Query: 361 EGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGG 420
           EGINFS L+ SPVYPLI GKSA K+S SEDSARICSE SMDEA VK KIVICES+ EGGG
Sbjct: 361 EGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGG 420

Query: 421 SDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVAT 480
           S WQ QAE V++LGGVG+VLIDD +KLVAEKF +P+T IS KDG E+L+YV+SSR PVAT
Sbjct: 421 SYWQGQAETVESLGGVGIVLIDDQAKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVAT 480

Query: 481 ILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATK 540
           ILPT T+INYKPAPAI YFSSRGPNP VLN+IKPDISAPGVNILAAWLGND++ TPQ   
Sbjct: 481 ILPTLTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540

Query: 541 SPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGS 600
           SPLFNVISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTTAIQTNNL SPMTLDTGS
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGS 600

Query: 601 VATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPK 660
           VATPYDYGAGEIST  AL+PGLVYETSTTDYL+YLCGRGY+ +TIKSI+ TVPDGFDCPK
Sbjct: 601 VATPYDYGAGEISTTEALKPGLVYETSTTDYLIYLCGRGYNRSTIKSISNTVPDGFDCPK 660

Query: 661 NSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPE 720
           +S ADYISNMNYPTIAVSELKG ESKK++RTVTNVGG+G TVY VSVDAP EV+VKVIPE
Sbjct: 661 DSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPE 720

Query: 721 KLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           KL+F+KNN+KQSY+VVFT TVSTLKK  FGSITW+NGKHRVRSPFVVTS
Sbjct: 721 KLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTS 765

BLAST of PI0012342 vs. ExPASy TrEMBL
Match: A0A6J1C938 (CO(2)-response secreted protease OS=Momordica charantia OX=3673 GN=LOC111009346 PE=3 SV=1)

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 636/771 (82.49%), Postives = 681/771 (88.33%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDFLRLLNSVN 60
           MQ I +   F    VI FLVS + K   AAE   NGVY+VYMG+AS SRTDFLRLL+SVN
Sbjct: 1   MQRIIYFSVFLFASVI-FLVSVHGKT--AAEAGNNGVYIVYMGTASASRTDFLRLLSSVN 60

Query: 61  R--RNAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQT 120
           R  RNAVVHTYKHGF+GFAARLSE E Q MRQ PGVVSVFPDP+LKLHTTHSWDFLVSQT
Sbjct: 61  RRNRNAVVHTYKHGFSGFAARLSEKETQEMRQTPGVVSVFPDPLLKLHTTHSWDFLVSQT 120

Query: 121 SVKIDANP-KSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDD 180
           SVKIDANP KSDPP SSSQPYD+IIGILDTGIWPESESFND GMGPIP RWKGTCMVG+D
Sbjct: 121 SVKIDANPTKSDPPASSSQPYDSIIGILDTGIWPESESFNDNGMGPIPLRWKGTCMVGND 180

Query: 181 FTSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGT 240
           FTSSNCN K+IGARFYESS+SD IRFHSPRD AGHGTHVASTAAGSA ANASYYGLAAGT
Sbjct: 181 FTSSNCNTKLIGARFYESSDSDEIRFHSPRDEAGHGTHVASTAAGSAAANASYYGLAAGT 240

Query: 241 AKGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPI 300
           AKGGSPGSRIAMYRVC  DGCRGS+I+ AFDD+IADGVDVLSLSLG+P  FRQ    DPI
Sbjct: 241 AKGGSPGSRIAMYRVCGTDGCRGSAIMAAFDDAIADGVDVLSLSLGSPYYFRQETKDDPI 300

Query: 301 AIGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIK 360
           AIGAFHAVEKGI VVCSAGNDGP+ G+VVNDAPWILTVAASTIDRDFESDVVLGN KVIK
Sbjct: 301 AIGAFHAVEKGIVVVCSAGNDGPTSGSVVNDAPWILTVAASTIDRDFESDVVLGNNKVIK 360

Query: 361 GEGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGG 420
           GEGINFS L+K+P YPLIQGKSAKKA ASEDSARICSEDSMDEA VK KIVICES+VEGG
Sbjct: 361 GEGINFSALKKTPEYPLIQGKSAKKADASEDSARICSEDSMDEALVKGKIVICESSVEGG 420

Query: 421 GSDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFP-TPMTVISKKDGPEILSYVNSSRKPV 480
           GSDWQ+Q E VK L G+G+VLIDD +KLVAEKF  + +T ISKKD  E+LSY NSSR P 
Sbjct: 421 GSDWQNQIETVKGLEGIGMVLIDDRAKLVAEKFDGSNITAISKKDSAEVLSYANSSRNPT 480

Query: 481 ATILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQA 540
           ATILPT T+INYKPAPA+ YFSSRGPNP +LNIIKPDISAPGVNILAAWLGNDS+ TPQ 
Sbjct: 481 ATILPTVTVINYKPAPAVAYFSSRGPNPAILNIIKPDISAPGVNILAAWLGNDSNSTPQG 540

Query: 541 TKSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDT 600
            K PLFNVISGTSMSCPHVSGVV  ++S+NP+WSPSAIKSAIMTTAIQTNNLGSPMTLDT
Sbjct: 541 KKPPLFNVISGTSMSCPHVSGVVGLLRSKNPSWSPSAIKSAIMTTAIQTNNLGSPMTLDT 600

Query: 601 GSVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDC 660
           GSVATPYDYGAGEIST GALQPGLVYET  TDYL YLC RGY+L+ IKSI+ TVPDGFDC
Sbjct: 601 GSVATPYDYGAGEISTTGALQPGLVYETDITDYLNYLCSRGYNLSIIKSISKTVPDGFDC 660

Query: 661 PKNSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVI 720
           PK  TADYISNMNYPTIAVSELKGKESKK+ RTVTNVGGNGETVYTVSVDA  E++V+VI
Sbjct: 661 PKKPTADYISNMNYPTIAVSELKGKESKKISRTVTNVGGNGETVYTVSVDAAGELDVRVI 720

Query: 721 PEKLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           PEKL+F KNN+KQSYQVVFT TV TLKK  FGSITWTNGK+RVRSPFVVTS
Sbjct: 721 PEKLEFTKNNQKQSYQVVFTSTVGTLKKDVFGSITWTNGKYRVRSPFVVTS 768

BLAST of PI0012342 vs. ExPASy TrEMBL
Match: A0A5D3E6D0 (CO(2)-response secreted protease isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007880 PE=3 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 552/581 (95.01%), Postives = 565/581 (97.25%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDFLRLLNSVN 60
           MQSISFCVF ALVCV F LVSENV+VADAAEDAKNGVY+VYMGSASGSR DFLRLLNSVN
Sbjct: 1   MQSISFCVFLALVCVTFCLVSENVEVADAAEDAKNGVYIVYMGSASGSRIDFLRLLNSVN 60

Query: 61  RRNAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSV 120
           RRNA+VHTYKHGFTGFAARLSEHEAQAMRQ+PGVVSVFPDPILKLHTTHSWDFLVSQTSV
Sbjct: 61  RRNALVHTYKHGFTGFAARLSEHEAQAMRQSPGVVSVFPDPILKLHTTHSWDFLVSQTSV 120

Query: 121 KIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFTS 180
           K+DANPKSD PTSSSQPYDTIIGILDTGIWPESESFND+GMGPIPSRWKGTCM+GDDFTS
Sbjct: 121 KVDANPKSDSPTSSSQPYDTIIGILDTGIWPESESFNDKGMGPIPSRWKGTCMIGDDFTS 180

Query: 181 SNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKG 240
           SNCNRKIIGARFYESSESDGIR+HSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKG
Sbjct: 181 SNCNRKIIGARFYESSESDGIRYHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKG 240

Query: 241 GSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIAIG 300
           GSP SRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLG+PS  R ++T DPIAIG
Sbjct: 241 GSPRSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGSPSFLRPDLTADPIAIG 300

Query: 301 AFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEG 360
           AFHAVEKGITVVCSAGNDGPS GTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEG
Sbjct: 301 AFHAVEKGITVVCSAGNDGPSSGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEG 360

Query: 361 INFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGGSD 420
           INFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVK KIVICES+VEGGGSD
Sbjct: 361 INFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKGKIVICESSVEGGGSD 420

Query: 421 WQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVATIL 480
           WQSQAE VK+LGGVGVVLIDDDSKLVAEKF TPMTVISKKDGPEILSYVNSSRKPVATIL
Sbjct: 421 WQSQAETVKSLGGVGVVLIDDDSKLVAEKFTTPMTVISKKDGPEILSYVNSSRKPVATIL 480

Query: 481 PTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATKSP 540
           PTETIINYKPAPAITYFSSRGPNP VLNIIKPDISAPGVNILAAWLGNDSS TPQATKSP
Sbjct: 481 PTETIINYKPAPAITYFSSRGPNPAVLNIIKPDISAPGVNILAAWLGNDSSSTPQATKSP 540

Query: 541 LFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTT 582
           LFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTT
Sbjct: 541 LFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTT 581

BLAST of PI0012342 vs. NCBI nr
Match: XP_004136374.1 (CO(2)-response secreted protease [Cucumis sativus] >KGN60051.1 hypothetical protein Csa_000867 [Cucumis sativus])

HSP 1 Score: 1434.1 bits (3711), Expect = 0.0e+00
Identity = 728/768 (94.79%), Postives = 748/768 (97.40%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSA-SGSRTDFLRLLNSV 60
           MQSISFCVFFALVCV FFLVSENVKVADAAEDA+NGVY+VYMGSA SG RTDFLRLLNSV
Sbjct: 1   MQSISFCVFFALVCVTFFLVSENVKVADAAEDARNGVYIVYMGSASSGFRTDFLRLLNSV 60

Query: 61  NRRNAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTS 120
           NRRNAVVHTYKHGFTGFAA LSEHEAQAMRQ+PGVVSVFPDP+LKLHTTHSWDFLVSQTS
Sbjct: 61  NRRNAVVHTYKHGFTGFAAHLSEHEAQAMRQSPGVVSVFPDPLLKLHTTHSWDFLVSQTS 120

Query: 121 VKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFT 180
           VKIDANPKSDPP SSSQPYDTIIGILDTGIWPESESFND GMGPIPSRWKGTCM GDDFT
Sbjct: 121 VKIDANPKSDPPASSSQPYDTIIGILDTGIWPESESFNDMGMGPIPSRWKGTCMTGDDFT 180

Query: 181 SSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240
           SSNCNRKIIGARFYESSESDGIR+HSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK
Sbjct: 181 SSNCNRKIIGARFYESSESDGIRYHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAK 240

Query: 241 GGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIAI 300
           GGSPGSRIAMYRVCMADGCRGSSI+KAFDDSIADGVDVLSLSLGTPS+FR ++T DPIAI
Sbjct: 241 GGSPGSRIAMYRVCMADGCRGSSIMKAFDDSIADGVDVLSLSLGTPSVFRPDLTADPIAI 300

Query: 301 GAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360
           GAFHAVEKGITVVCSAGNDGPS GTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE
Sbjct: 301 GAFHAVEKGITVVCSAGNDGPSSGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGE 360

Query: 361 GINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGGS 420
           GINFSDLQKSPVYPLI+GKSAKKAS SEDSARICSEDSMDEAQVK KIVICE++VEGGGS
Sbjct: 361 GINFSDLQKSPVYPLIEGKSAKKASDSEDSARICSEDSMDEAQVKGKIVICENSVEGGGS 420

Query: 421 DWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVATI 480
           DWQSQAE VKNLGGVG+VLIDDDSKLVAEKF TPMTVISKKDG EILSYVNSSRKPVAT+
Sbjct: 421 DWQSQAETVKNLGGVGLVLIDDDSKLVAEKFSTPMTVISKKDGLEILSYVNSSRKPVATV 480

Query: 481 LPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATKS 540
           LPTETIINYKPAPAITYFSSRGPNP VLNIIKPDISAPGVNILAAWLGNDSS TPQATKS
Sbjct: 481 LPTETIINYKPAPAITYFSSRGPNPAVLNIIKPDISAPGVNILAAWLGNDSSSTPQATKS 540

Query: 541 PLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGSV 600
           PLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAI+SAIMTTAIQTNNLGSPMTLDTGSV
Sbjct: 541 PLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIRSAIMTTAIQTNNLGSPMTLDTGSV 600

Query: 601 ATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPKN 660
           ATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGY+LTTIKSITTT+PDGFDCPKN
Sbjct: 601 ATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYNLTTIKSITTTIPDGFDCPKN 660

Query: 661 STADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPEK 720
           S ADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAP+EVEVKVIPEK
Sbjct: 661 SNADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPQEVEVKVIPEK 720

Query: 721 LKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           LKFAKN EKQSYQVVFTPTVST+K+ GFGSITWTNGKHRVRSPFVVTS
Sbjct: 721 LKFAKNYEKQSYQVVFTPTVSTMKR-GFGSITWTNGKHRVRSPFVVTS 767

BLAST of PI0012342 vs. NCBI nr
Match: XP_038896398.1 (CO(2)-response secreted protease [Benincasa hispida])

HSP 1 Score: 1336.6 bits (3458), Expect = 0.0e+00
Identity = 689/771 (89.36%), Postives = 718/771 (93.13%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDA--KNGVYVVYMGSASGSRTDFLRLLNS 60
           MQSISFCVFF  VCVIF LVSEN K+  AA +    NGVY+VYMGSAS SRTDFLRLLNS
Sbjct: 1   MQSISFCVFFVFVCVIFSLVSENAKIVTAAAEGPKNNGVYIVYMGSASASRTDFLRLLNS 60

Query: 61  VNRRN--AVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVS 120
           V+RRN  AVVHTYKHGFTGFAA LSE EAQAMRQ+PGVVSVFPDPILKLHTTHSWDFLVS
Sbjct: 61  VHRRNGKAVVHTYKHGFTGFAAHLSEQEAQAMRQSPGVVSVFPDPILKLHTTHSWDFLVS 120

Query: 121 QTSVKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGD 180
           QTSVKIDANPKSD   SS QPYDTIIGILDTGIWPESESF+D+GM PIPSRWKGTCMVGD
Sbjct: 121 QTSVKIDANPKSD---SSPQPYDTIIGILDTGIWPESESFSDKGMSPIPSRWKGTCMVGD 180

Query: 181 DFTSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAG 240
           DFTSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAG
Sbjct: 181 DFTSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAG 240

Query: 241 TAKGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDP 300
           TAKGGSPGSRIAMYRVC+ADGC GSSI+ AFDD+IADGVDVLSLSLG PS FR ++T DP
Sbjct: 241 TAKGGSPGSRIAMYRVCVADGCHGSSIMAAFDDAIADGVDVLSLSLGAPSYFRPDLTADP 300

Query: 301 IAIGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVI 360
           IAIGAFHAVEKGITVVCSAGNDGPS G+VVNDAPWI+TVAASTIDRDFESDVVLGNKKVI
Sbjct: 301 IAIGAFHAVEKGITVVCSAGNDGPSSGSVVNDAPWIITVAASTIDRDFESDVVLGNKKVI 360

Query: 361 KGEGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEG 420
           KGEGINFS+LQKSPVYPLIQGKSAKKASASEDSARICSEDSMDE  VK KIVICE++VEG
Sbjct: 361 KGEGINFSNLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDETHVKGKIVICENSVEG 420

Query: 421 GGSDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPV 480
           GGSDWQ QAE VKNLGGVGVVLIDD+SKLVAEKFP+PMTVISKKDG E+LSYV SS  PV
Sbjct: 421 GGSDWQDQAETVKNLGGVGVVLIDDNSKLVAEKFPSPMTVISKKDGAELLSYVTSSGNPV 480

Query: 481 ATILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQA 540
           ATILPT TIINYKPAPAITYFSSRGPNP VLN+IKPDISAPGVNILAAWLGNDSS TPQA
Sbjct: 481 ATILPTVTIINYKPAPAITYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDSSSTPQA 540

Query: 541 TKSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDT 600
           TKSPLFNVISGTSMSCPHVSGVVA VKSQNPTWSPSAI+SAIMTTAIQTNNLGSPMTLDT
Sbjct: 541 TKSPLFNVISGTSMSCPHVSGVVAFVKSQNPTWSPSAIRSAIMTTAIQTNNLGSPMTLDT 600

Query: 601 GSVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDC 660
           GSVATPYDYGAGEISTNGALQPGLVYETSTTDYL YLCGRGY+ +TIKSI+TTVPD FDC
Sbjct: 601 GSVATPYDYGAGEISTNGALQPGLVYETSTTDYLNYLCGRGYNRSTIKSISTTVPDEFDC 660

Query: 661 PKNSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVI 720
           PKNSTA YISNMNYPTIAVSELKGKESKKVIRTVTNVGG+GE VYTVSVDAP EV+VKVI
Sbjct: 661 PKNSTAYYISNMNYPTIAVSELKGKESKKVIRTVTNVGGDGEAVYTVSVDAPGEVDVKVI 720

Query: 721 PEKLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           PE LKF KNNEKQSYQVVFT TVSTL +  FGSITWT+GKHRVRSPFVVTS
Sbjct: 721 PEILKFGKNNEKQSYQVVFTSTVSTLNE-VFGSITWTDGKHRVRSPFVVTS 767

BLAST of PI0012342 vs. NCBI nr
Match: XP_022937005.1 (CO(2)-response secreted protease [Cucurbita moschata])

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 654/769 (85.05%), Postives = 698/769 (90.77%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDFLRLLNSVN 60
           MQ+IS CVF  LVCVIF L+SEN K+A AA    NGVY+VYMGSA  SRTDFLRLL SVN
Sbjct: 1   MQTISSCVFL-LVCVIFLLLSENAKIA-AAAAGNNGVYIVYMGSA--SRTDFLRLLTSVN 60

Query: 61  RR--NAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQT 120
           RR  NAVV TYKHGFTGFAARLSE EA AMRQ PGVVSVFPDPILKLHTTHSWDFLVSQT
Sbjct: 61  RRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQT 120

Query: 121 SVKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDF 180
           SVKID+ PK+DPPTSSSQP+DTIIGILDTGIWPESESF DEGM PIPSRWKGTCM GDDF
Sbjct: 121 SVKIDSAPKADPPTSSSQPFDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 180

Query: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240
           TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA
Sbjct: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240

Query: 241 KGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIA 300
           KGGSPGSRIAMYRVCMADGC GSSIL AFDD+I DGVDVLSLSLG P+ ++  +T DPIA
Sbjct: 241 KGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPNYYKPELTTDPIA 300

Query: 301 IGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360
           IGAFHAVEKGITVVCSAGNDGP+ G+VVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG
Sbjct: 301 IGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360

Query: 361 EGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGG 420
           EGINFS L+ SPVYPLI GKSA K+S SEDSARICSE SMDEA VK KIVICES+ EGGG
Sbjct: 361 EGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGG 420

Query: 421 SDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVAT 480
           S WQ QAE V++LGGVG+VLIDD +KLVAEKF +P+T IS KDG E+L+YV+SSR PVAT
Sbjct: 421 SYWQGQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVAT 480

Query: 481 ILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATK 540
           ILPT T+INYKPAPAI YFSSRGPNP VLN+IKPDISAPGVNILAAWLGND++ TPQ   
Sbjct: 481 ILPTLTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540

Query: 541 SPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGS 600
           SPLFNVISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTTAIQTNNL SPMTLDTGS
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGS 600

Query: 601 VATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPK 660
           VATPYDYGAGEIST GAL+PGLVYETSTTDYL+YLCGRGY+ +TIKSI  TVPDGFDCPK
Sbjct: 601 VATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPK 660

Query: 661 NSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPE 720
           +S ADYISNMNYPTIAVSELKG ESKK++RTVTNVGG+G TVY VSVDAP EV+VKVIPE
Sbjct: 661 DSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPE 720

Query: 721 KLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           KL+F+KNN+KQSY+VVFT TVSTLKK  FGSITW+NGKHRVRSPFVVTS
Sbjct: 721 KLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTS 765

BLAST of PI0012342 vs. NCBI nr
Match: KAG6591258.1 (CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1276.5 bits (3302), Expect = 0.0e+00
Identity = 654/769 (85.05%), Postives = 697/769 (90.64%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDFLRLLNSVN 60
           MQ+IS CVF  LVCVIF L+SEN K+A AA    NGVY+VYMGSA  SRTDFLRLL SVN
Sbjct: 1   MQTISSCVFL-LVCVIFLLLSENAKIA-AAAAGNNGVYIVYMGSA--SRTDFLRLLTSVN 60

Query: 61  RR--NAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQT 120
           RR  NAVV TYKHGFTGFAARLSE EA AMRQ PGVVSVFPDPILKLHTTHSWDFLVSQT
Sbjct: 61  RRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQT 120

Query: 121 SVKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDF 180
           SVKID+ PK+DPPTSSSQP DTIIGILDTGIWPESESF DEGM PIPSRWKGTCM GDDF
Sbjct: 121 SVKIDSAPKADPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 180

Query: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240
           TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA
Sbjct: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240

Query: 241 KGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIA 300
           KGGSPGSRIAMYRVCMADGC GSSIL AFDD+I DGVDVLSLSLG P+ ++  +T DPIA
Sbjct: 241 KGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGAPTYYKPELTTDPIA 300

Query: 301 IGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360
           IGAFHAVEKGITVVCSAGNDGP+ G+VVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG
Sbjct: 301 IGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360

Query: 361 EGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGG 420
           EGINFS L+ SPVYPLI GKSA K+S SEDSARICSE SMDEA VK KIVICES+ EGGG
Sbjct: 361 EGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGG 420

Query: 421 SDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVAT 480
           S WQ QAE V++LGGVG+VLIDD +KLVAEKF +P+T IS KDG E+L+YV+SSR PVAT
Sbjct: 421 SYWQGQAETVESLGGVGIVLIDDQTKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVAT 480

Query: 481 ILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATK 540
           ILPT T+INYKPAPAI YFSSRGPNP VLN+IKPDISAPGVNILAAWLGND++ TPQ   
Sbjct: 481 ILPTLTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540

Query: 541 SPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGS 600
           SPLFNVISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTTAIQTNNL SPMTLDTGS
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGS 600

Query: 601 VATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPK 660
           VATPYDYGAGEIST GAL+PGLVYETSTTDYL+YLCGRGY+ +TIKSI  TVPDGFDCPK
Sbjct: 601 VATPYDYGAGEISTTGALKPGLVYETSTTDYLIYLCGRGYNRSTIKSIANTVPDGFDCPK 660

Query: 661 NSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPE 720
           +S ADYISNMNYPTIAVSELKG ESKK++RTVTNVGG+G TVY VSVDAP EV+VKVIPE
Sbjct: 661 DSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPE 720

Query: 721 KLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           KL+F+KNN+KQSY+VVFT TVSTLKK  FGSITW+NGKHRVRSPFVVTS
Sbjct: 721 KLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTS 765

BLAST of PI0012342 vs. NCBI nr
Match: XP_022975820.1 (CO(2)-response secreted protease [Cucurbita maxima])

HSP 1 Score: 1271.1 bits (3288), Expect = 0.0e+00
Identity = 652/769 (84.79%), Postives = 695/769 (90.38%), Query Frame = 0

Query: 1   MQSISFCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDFLRLLNSVN 60
           MQ+IS C F  LVCVIF L+SEN K+A AA    NGVY+VYMGSA  SRTDFLRLL SVN
Sbjct: 1   MQTISSCAFL-LVCVIFLLLSENAKIA-AAAAGNNGVYIVYMGSA--SRTDFLRLLTSVN 60

Query: 61  RR--NAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQT 120
           RR  NAVV TYKHGFTGFAARLSE EA AMRQ PGVVSVFPDPILKLHTTHSWDFLVSQT
Sbjct: 61  RRNGNAVVRTYKHGFTGFAARLSEQEAHAMRQRPGVVSVFPDPILKLHTTHSWDFLVSQT 120

Query: 121 SVKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDF 180
           SVKID+ PKS+PPTSSSQP DTIIGILDTGIWPESESF DEGM PIPSRWKGTCM GDDF
Sbjct: 121 SVKIDSAPKSEPPTSSSQPSDTIIGILDTGIWPESESFRDEGMPPIPSRWKGTCMAGDDF 180

Query: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240
           TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA
Sbjct: 181 TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTA 240

Query: 241 KGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIA 300
           KGGSPGSRIAMYRVCMADGC GSSIL AFDD+I DGVDVLSLSLGTP  ++  +  DPIA
Sbjct: 241 KGGSPGSRIAMYRVCMADGCHGSSILAAFDDAINDGVDVLSLSLGTPYFYKPELATDPIA 300

Query: 301 IGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360
           IGAFHAVEKGITVVCSAGNDGP+ G+VVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG
Sbjct: 301 IGAFHAVEKGITVVCSAGNDGPTPGSVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 360

Query: 361 EGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGG 420
           EGINFS L+ SPVYPLI GKSA K+S SEDSARICSE SMDEA VK KIVICES+ EGGG
Sbjct: 361 EGINFSALESSPVYPLIHGKSANKSSTSEDSARICSEGSMDEALVKGKIVICESSEEGGG 420

Query: 421 SDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPTPMTVISKKDGPEILSYVNSSRKPVAT 480
           S WQ QAE V++LGGVG+VLIDD +KLVAEKF +P+T IS KDG E+L+YV+SSR PVAT
Sbjct: 421 SYWQGQAETVESLGGVGIVLIDDQAKLVAEKFTSPITAISSKDGDEVLAYVHSSRNPVAT 480

Query: 481 ILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATK 540
           ILPT T+INYKPAPAI YFSSRGPNP VLN+IKPDISAPGVNILAAWLGND++ TPQ   
Sbjct: 481 ILPTLTVINYKPAPAIAYFSSRGPNPAVLNMIKPDISAPGVNILAAWLGNDTNSTPQPEN 540

Query: 541 SPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGS 600
           SPLFNVISGTSMSCPHVSGVVA VKSQNPTWSPSAIKSAIMTTAIQTNNL SPMTLDTGS
Sbjct: 541 SPLFNVISGTSMSCPHVSGVVAMVKSQNPTWSPSAIKSAIMTTAIQTNNLESPMTLDTGS 600

Query: 601 VATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPK 660
           VATPYDYGAGEIST  AL+PGLVYETSTTDYL+YLCGRGY+ +TIKSI+ TVPDGFDCPK
Sbjct: 601 VATPYDYGAGEISTTEALKPGLVYETSTTDYLIYLCGRGYNRSTIKSISNTVPDGFDCPK 660

Query: 661 NSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPE 720
           +S ADYISNMNYPTIAVSELKG ESKK++RTVTNVGG+G TVY VSVDAP EV+VKVIPE
Sbjct: 661 DSNADYISNMNYPTIAVSELKGSESKKIVRTVTNVGGDGATVYKVSVDAPGEVDVKVIPE 720

Query: 721 KLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           KL+F+KNN+KQSY+VVFT TVSTLKK  FGSITW+NGKHRVRSPFVVTS
Sbjct: 721 KLEFSKNNQKQSYKVVFTSTVSTLKKEAFGSITWSNGKHRVRSPFVVTS 765

BLAST of PI0012342 vs. TAIR 10
Match: AT1G20160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 877.5 bits (2266), Expect = 8.0e-255
Identity = 459/768 (59.77%), Postives = 574/768 (74.74%), Query Frame = 0

Query: 6   FCVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSAS----GSRTDFLRLLNSVNR 65
           F  F  L+C++F   +E      A     +GVY+VYMGSAS     +R   L       R
Sbjct: 10  FLSFLYLLCILFMTETE------AGSRNGDGVYIVYMGSASSAANANRAQILINTMFKRR 69

Query: 66  RNAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSVK 125
            N ++HTYKHGF+GFAARL+  EA+ + + PGVVSVFPDP  +LHTTHSWDFL  QTSVK
Sbjct: 70  ANDLLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVK 129

Query: 126 IDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFTSS 185
           +D+ P   P ++S   YD+I+GILDTGIWPESESFND+ MGPIPSRWKGTCM   DF SS
Sbjct: 130 VDSGP---PSSASDGSYDSIVGILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKSS 189

Query: 186 NCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKGG 245
           NCNRKIIGAR+Y++ + D   +++ RD  GHG+HV+ST AGSAV NASYYG+A+GTAKGG
Sbjct: 190 NCNRKIIGARYYKNPDDDS-EYYTTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGG 249

Query: 246 SPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPIAIGA 305
           S  +RIAMY+VC   GC GSSIL AFDD+IADGVDVLSLSLG P+  R ++  DPIAIGA
Sbjct: 250 SQNARIAMYKVCNPGGCTGSSILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGA 309

Query: 306 FHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKGEGI 365
           FHAVE+GI V+CSAGNDGP  GTV N APWI+TVAA+TIDRDFESDVVLG  KVIKGEGI
Sbjct: 310 FHAVEQGILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGI 369

Query: 366 NFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGGSDW 425
           +FS++ KSPVYPLI GKSAK A ASE SAR C  DS+D+ +VK KIV+CE NV  GGS +
Sbjct: 370 HFSNVSKSPVYPLIHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCE-NV--GGSYY 429

Query: 426 QSQA-EAVKNLGGVGVVLIDDDSKLVAEKFPT-PMTVISKKDGPEILSYVNSSRKPVATI 485
            S A + VK+ GG G V +DD ++ VA  + + P TVI  K+  EI SY+NS++ PVATI
Sbjct: 430 ASSARDEVKSKGGTGCVFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATI 489

Query: 486 LPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQATKS 545
           LPT T+  + PAPA+ YFSSRGP+    +I+KPDI+APGV+ILAAW GNDSS + +   +
Sbjct: 490 LPTATVEKFTPAPAVAYFSSRGPSSLTRSILKPDITAPGVSILAAWTGNDSSISLEGKPA 549

Query: 546 PLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDTGSV 605
             +NVISGTSM+ PHVS V + +KSQ+PTW PSAI+SAIMTTA QTNN    +T +TG+ 
Sbjct: 550 SQYNVISGTSMAAPHVSAVASLIKSQHPTWGPSAIRSAIMTTATQTNNDKGLITTETGAT 609

Query: 606 ATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDCPKN 665
           ATPYD GAGE+S+  ++QPGLVYET+ TDYL +LC  GY++TTIK+++   P+ F CP +
Sbjct: 610 ATPYDSGAGELSSTASMQPGLVYETTETDYLNFLCYYGYNVTTIKAMSKAFPENFTCPAD 669

Query: 666 STADYISNMNYPTIAVSELKGKESKKVIRTVTNVGGNGETVYTVSVDAPEEVEVKVIPEK 725
           S  D IS +NYP+I +S  KG  SK V RTVTNVG +GE VYTVSV+ P    ++V PEK
Sbjct: 670 SNLDLISTINYPSIGISGFKGNGSKTVTRTVTNVGEDGEAVYTVSVETPPGFNIQVTPEK 729

Query: 726 LKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           L+F K+ EK +YQV+ + T S LK+  FG++TW+N K++VRSP V++S
Sbjct: 730 LQFTKDGEKLTYQVIVSATAS-LKQDVFGALTWSNAKYKVRSPIVISS 763

BLAST of PI0012342 vs. TAIR 10
Match: AT1G20160.2 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 862.8 bits (2228), Expect = 2.0e-250
Identity = 447/732 (61.07%), Postives = 556/732 (75.96%), Query Frame = 0

Query: 42  MGSAS----GSRTDFLRLLNSVNRRNAVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSV 101
           MGSAS     +R   L       R N ++HTYKHGF+GFAARL+  EA+ + + PGVVSV
Sbjct: 1   MGSASSAANANRAQILINTMFKRRANDLLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSV 60

Query: 102 FPDPILKLHTTHSWDFLVSQTSVKIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFN 161
           FPDP  +LHTTHSWDFL  QTSVK+D+ P   P ++S   YD+I+GILDTGIWPESESFN
Sbjct: 61  FPDPHFQLHTTHSWDFLKYQTSVKVDSGP---PSSASDGSYDSIVGILDTGIWPESESFN 120

Query: 162 DEGMGPIPSRWKGTCMVGDDFTSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVA 221
           D+ MGPIPSRWKGTCM   DF SSNCNRKIIGAR+Y++ + D   +++ RD  GHG+HV+
Sbjct: 121 DKDMGPIPSRWKGTCMEAKDFKSSNCNRKIIGARYYKNPDDDS-EYYTTRDVIGHGSHVS 180

Query: 222 STAAGSAVANASYYGLAAGTAKGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDV 281
           ST AGSAV NASYYG+A+GTAKGGS  +RIAMY+VC   GC GSSIL AFDD+IADGVDV
Sbjct: 181 STIAGSAVENASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGSSILAAFDDAIADGVDV 240

Query: 282 LSLSLGTPSIFRQNMTVDPIAIGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAA 341
           LSLSLG P+  R ++  DPIAIGAFHAVE+GI V+CSAGNDGP  GTV N APWI+TVAA
Sbjct: 241 LSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPDGGTVTNTAPWIMTVAA 300

Query: 342 STIDRDFESDVVLGNKKVIKGEGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDS 401
           +TIDRDFESDVVLG  KVIKGEGI+FS++ KSPVYPLI GKSAK A ASE SAR C  DS
Sbjct: 301 NTIDRDFESDVVLGGNKVIKGEGIHFSNVSKSPVYPLIHGKSAKSADASEGSARACDSDS 360

Query: 402 MDEAQVKRKIVICESNVEGGGSDWQSQA-EAVKNLGGVGVVLIDDDSKLVAEKFPT-PMT 461
           +D+ +VK KIV+CE NV  GGS + S A + VK+ GG G V +DD ++ VA  + + P T
Sbjct: 361 LDQEKVKGKIVLCE-NV--GGSYYASSARDEVKSKGGTGCVFVDDRTRAVASAYGSFPTT 420

Query: 462 VISKKDGPEILSYVNSSRKPVATILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDIS 521
           VI  K+  EI SY+NS++ PVATILPT T+  + PAPA+ YFSSRGP+    +I+KPDI+
Sbjct: 421 VIDSKEAAEIFSYLNSTKDPVATILPTATVEKFTPAPAVAYFSSRGPSSLTRSILKPDIT 480

Query: 522 APGVNILAAWLGNDSSPTPQATKSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIK 581
           APGV+ILAAW GNDSS + +   +  +NVISGTSM+ PHVS V + +KSQ+PTW PSAI+
Sbjct: 481 APGVSILAAWTGNDSSISLEGKPASQYNVISGTSMAAPHVSAVASLIKSQHPTWGPSAIR 540

Query: 582 SAIMTTAIQTNNLGSPMTLDTGSVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCG 641
           SAIMTTA QTNN    +T +TG+ ATPYD GAGE+S+  ++QPGLVYET+ TDYL +LC 
Sbjct: 541 SAIMTTATQTNNDKGLITTETGATATPYDSGAGELSSTASMQPGLVYETTETDYLNFLCY 600

Query: 642 RGYDLTTIKSITTTVPDGFDCPKNSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVGG 701
            GY++TTIK+++   P+ F CP +S  D IS +NYP+I +S  KG  SK V RTVTNVG 
Sbjct: 601 YGYNVTTIKAMSKAFPENFTCPADSNLDLISTINYPSIGISGFKGNGSKTVTRTVTNVGE 660

Query: 702 NGETVYTVSVDAPEEVEVKVIPEKLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNG 761
           +GE VYTVSV+ P    ++V PEKL+F K+ EK +YQV+ + T S LK+  FG++TW+N 
Sbjct: 661 DGEAVYTVSVETPPGFNIQVTPEKLQFTKDGEKLTYQVIVSATAS-LKQDVFGALTWSNA 720

Query: 762 KHRVRSPFVVTS 768
           K++VRSP V++S
Sbjct: 721 KYKVRSPIVISS 724

BLAST of PI0012342 vs. TAIR 10
Match: AT1G20150.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 768.8 bits (1984), Expect = 4.0e-222
Identity = 410/777 (52.77%), Postives = 552/777 (71.04%), Query Frame = 0

Query: 7   CVFFALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSAS---GSRTDFLRLLNSVNRRN 66
           C+   ++  +FF +S   K    +E +K+G Y++YMG+AS    +  D + LL+S+ +R+
Sbjct: 4   CLTITIMFFMFFFLSVIQKC--KSETSKSGDYIIYMGAASSDGSTDNDHVELLSSLLQRS 63

Query: 67  --AVVHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSVK 126
               +H YKHGF+GFAA LSE EA  + + PGV+SVFPD +L+LHTT SWDFLV Q S +
Sbjct: 64  GKTPMHRYKHGFSGFAAHLSEDEAHLIAKQPGVLSVFPDQMLQLHTTRSWDFLV-QESYQ 123

Query: 127 IDA--NPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDF- 186
            D      +    S     DTIIG LD+GIWPE++SFND  MGP+P +WKGTCM G    
Sbjct: 124 RDTYFTEMNYEQESEMHEGDTIIGFLDSGIWPEAQSFNDRHMGPVPEKWKGTCMRGKKTQ 183

Query: 187 -TSSNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGT 246
             S  CNRK+IGAR+Y SS      + +PRD  GHGTHVAS AAG  +ANASYYGLA+G 
Sbjct: 184 PDSFRCNRKLIGARYYNSSFFLDPDYETPRDFLGHGTHVASIAAGQIIANASYYGLASGI 243

Query: 247 AKGGSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLGTPSIFRQNMTVDPI 306
            +GGSP SRIAMYR C   GCRGSSIL AFDD+IADGVDV+S+S+G   ++  N+  DP+
Sbjct: 244 MRGGSPSSRIAMYRACSLLGCRGSSILAAFDDAIADGVDVISISMG---LWPDNLLEDPL 303

Query: 307 AIGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLG--NKKV 366
           +IG+FHAVE+GITVVCS GN GPS  +V N APW++TVAASTIDR FES+++LG    ++
Sbjct: 304 SIGSFHAVERGITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDENRL 363

Query: 367 IKGEGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVE 426
           I+G GIN +++ K+  YPLI  +SAKK  A+E++AR C+ D++D+  VK KIV+C+S+++
Sbjct: 364 IEGFGINIANIDKTQAYPLIHARSAKKIDANEEAARNCAPDTLDQTIVKGKIVVCDSDLD 423

Query: 427 GGGSDWQSQAEAVKNLGGVGVVLIDDDSKLVAEKFPT-PMTVISKKDGPEILSYVNSSRK 486
                W+S  + VK LGG+G+VL+DD+S  ++   P+  +T+I  +DG +I+SY+NS+R+
Sbjct: 424 NQVIQWKS--DEVKRLGGIGMVLVDDESMDLSFIDPSFLVTIIKPEDGIQIMSYINSTRE 483

Query: 487 PVATILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTP 546
           P+ATI+PT +   +  AP+I  FSSRGP     +I+KPDI+APGVNILA+WL  D +  P
Sbjct: 484 PIATIMPTRSRTGHMLAPSIPSFSSRGPYLLTRSILKPDIAAPGVNILASWLVGDRNAAP 543

Query: 547 QATKSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTL 606
           +    PLFN+ SGTSMSCPHVSG+ A +KS+ P+WSP+AI+SAIMTTA+Q  N GS +T 
Sbjct: 544 EGKPPPLFNIESGTSMSCPHVSGIAARLKSRYPSWSPAAIRSAIMTTAVQMTNTGSHITT 603

Query: 607 DTGSVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGF 666
           +TG  ATPYD+GAG+++  G   PGL+YET+  DYL +L   G+    IK I+  +P GF
Sbjct: 604 ETGEKATPYDFGAGQVTIFGPSSPGLIYETNHMDYLNFLGYYGFTSDQIKKISNRIPQGF 663

Query: 667 DCPKNSTADYISNMNYPTIAVSELKGKESKKVIRTVTNVG----GNGETVYTVSVDAPEE 726
            CP+ S    ISN+NYP+I++S   GKES++V RTVTNV     G+ +TVYTVS+DAPE 
Sbjct: 664 ACPEQSNRGDISNINYPSISISNFNGKESRRVSRTVTNVASRLIGDEDTVYTVSIDAPEG 723

Query: 727 VEVKVIPEKLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVVTS 768
           + V+VIP +L F K  +K SYQV+F+ T + LK   FGSITW+NG + VRSPFVVTS
Sbjct: 724 LLVRVIPRRLHFRKIGDKLSYQVIFSSTTTILKDDAFGSITWSNGMYNVRSPFVVTS 772

BLAST of PI0012342 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 523.5 bits (1347), Expect = 2.9e-148
Identity = 333/773 (43.08%), Postives = 449/773 (58.09%), Query Frame = 0

Query: 11  ALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDF------LRLLNSVNRRNA 70
           A  C+  +L+   +    A  D    VY+VYMGS S SR D+      + +L  V   ++
Sbjct: 6   ASTCLYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLS-SRADYIPTSDHMSILQQVTGESS 65

Query: 71  V----VHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSV 130
           +    V +YK  F GFAARL+E E   + +  GVVSVFP+ IL+LHTT SWDF+     V
Sbjct: 66  IEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFM----GV 125

Query: 131 KIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFTS 190
           K   N K +    S    DTIIG++DTGIWPES+SF+D+G GP P +WKG C  G +FT 
Sbjct: 126 KEGKNTKRNLAIES----DTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT- 185

Query: 191 SNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKG 250
             CN K+IGAR Y S           RD +GHGTH ASTAAG+AV + S++G+  GT +G
Sbjct: 186 --CNNKLIGARDYTS--------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 245

Query: 251 GSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLG--TPSIFRQNMTVDPIA 310
           G P SRIA Y+VC   GC   ++L +FDD+IADGVD++++S+G   PSIF      DPIA
Sbjct: 246 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFED----DPIA 305

Query: 311 IGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 370
           IGAFHA+ KGI  V SAGN GP   TV + APWI TVAAST +R F + VVLGN K + G
Sbjct: 306 IGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAG 365

Query: 371 EGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGG 430
             +N  D+ K   YPL+ GKSA  ++    +A +C+   +++++VK KI++C     GG 
Sbjct: 366 RSVNAFDM-KGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVC-----GGP 425

Query: 431 SDWQSQAEAVKNLGGVGVVLIDDDSKL--VAEKFPTPMTVISKKDGPEILSYVNSSRKPV 490
           S +       K    VG + I D S    VA     P + +  KD   ++SY+ S   P 
Sbjct: 426 SGY-------KIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQ 485

Query: 491 ATILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQA 550
           A +L TETI N + +P I  FSSRGPN   ++I+KPDI+APGV ILAA+  N   P+   
Sbjct: 486 AAVLKTETIFN-RTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPN-GEPSEDD 545

Query: 551 TKSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDT 610
           T+   ++V SGTSM+CPHV+GV A VK+  P WSPS I+SAIMTTA      G       
Sbjct: 546 TRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKG------R 605

Query: 611 GSVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDC 670
           G  +T + YGAG +    AL PGLVYE    D++ +LCG  Y   T+K I+    D   C
Sbjct: 606 GIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIIS---GDTVKC 665

Query: 671 PKNSTADYISNMNYPTIAVSELKGKESKKVI---RTVTNVGGNGETVYT-VSVDAPEEVE 730
            K +      N+NYP+++ ++L G +S   +   RT+TNVG    T  + V      ++ 
Sbjct: 666 SKKNKI-LPRNLNYPSMS-AKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLS 725

Query: 731 VKVIPEKLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVV 766
           +KV P  L F   NEKQS+ V  T +    +     ++ W++G H VRSP VV
Sbjct: 726 IKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVV 728

BLAST of PI0012342 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 521.5 bits (1342), Expect = 1.1e-147
Identity = 332/773 (42.95%), Postives = 448/773 (57.96%), Query Frame = 0

Query: 11  ALVCVIFFLVSENVKVADAAEDAKNGVYVVYMGSASGSRTDF------LRLLNSVNRRNA 70
           A  C+  +L+   +    A  D    VY+VYMGS S SR D+      + +L  V   ++
Sbjct: 6   ASTCLYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLS-SRADYIPTSDHMSILQQVTGESS 65

Query: 71  V----VHTYKHGFTGFAARLSEHEAQAMRQNPGVVSVFPDPILKLHTTHSWDFLVSQTSV 130
           +    V +YK  F GFAARL+E E   + +  GVVSVFP+ IL+LHTT SWDF+     V
Sbjct: 66  IEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFM----GV 125

Query: 131 KIDANPKSDPPTSSSQPYDTIIGILDTGIWPESESFNDEGMGPIPSRWKGTCMVGDDFTS 190
           K   N K +    S    DTIIG++DTGIWPES+SF+D+G GP P +WKG C  G +FT 
Sbjct: 126 KEGKNTKRNLAIES----DTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT- 185

Query: 191 SNCNRKIIGARFYESSESDGIRFHSPRDGAGHGTHVASTAAGSAVANASYYGLAAGTAKG 250
             CN K+IGAR Y S           RD +GHGTH ASTAAG+AV + S++G+  GT +G
Sbjct: 186 --CNNKLIGARDYTS--------EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 245

Query: 251 GSPGSRIAMYRVCMADGCRGSSILKAFDDSIADGVDVLSLSLG--TPSIFRQNMTVDPIA 310
           G P SRIA Y+VC   GC   ++L +FDD+IADGVD++++S+G   PSIF      DPIA
Sbjct: 246 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFED----DPIA 305

Query: 311 IGAFHAVEKGITVVCSAGNDGPSYGTVVNDAPWILTVAASTIDRDFESDVVLGNKKVIKG 370
           IGAFHA+ KGI  V SAGN GP   TV + APWI TVAAST +R F + VVLGN K + G
Sbjct: 306 IGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAG 365

Query: 371 EGINFSDLQKSPVYPLIQGKSAKKASASEDSARICSEDSMDEAQVKRKIVICESNVEGGG 430
             +N  D+ K   YPL+ GKSA  ++    +A +C+   +++++VK KI++C     GG 
Sbjct: 366 RSVNAFDM-KGKKYPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVC-----GGP 425

Query: 431 SDWQSQAEAVKNLGGVGVVLIDDDSKL--VAEKFPTPMTVISKKDGPEILSYVNSSRKPV 490
           S +       K    VG + I D S    VA     P + +  KD   ++SY+ S   P 
Sbjct: 426 SGY-------KIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQ 485

Query: 491 ATILPTETIINYKPAPAITYFSSRGPNPEVLNIIKPDISAPGVNILAAWLGNDSSPTPQA 550
           A +L TETI N + +P I  FSSRGPN   ++I+KPDI+APGV ILAA+  N   P+   
Sbjct: 486 AAVLKTETIFN-RTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPN-GEPSEDD 545

Query: 551 TKSPLFNVISGTSMSCPHVSGVVASVKSQNPTWSPSAIKSAIMTTAIQTNNLGSPMTLDT 610
           T+   ++V SGTSM+CPHV+GV A VK+  P WSPS I+SAIMTTA              
Sbjct: 546 TRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTA-----------KGR 605

Query: 611 GSVATPYDYGAGEISTNGALQPGLVYETSTTDYLLYLCGRGYDLTTIKSITTTVPDGFDC 670
           G  +T + YGAG +    AL PGLVYE    D++ +LCG  Y   T+K I+    D   C
Sbjct: 606 GIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIIS---GDTVKC 665

Query: 671 PKNSTADYISNMNYPTIAVSELKGKESKKVI---RTVTNVGGNGETVYT-VSVDAPEEVE 730
            K +      N+NYP+++ ++L G +S   +   RT+TNVG    T  + V      ++ 
Sbjct: 666 SKKNKI-LPRNLNYPSMS-AKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLS 723

Query: 731 VKVIPEKLKFAKNNEKQSYQVVFTPTVSTLKKGGFGSITWTNGKHRVRSPFVV 766
           +KV P  L F   NEKQS+ V  T +    +     ++ W++G H VRSP VV
Sbjct: 726 IKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVV 723

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LNU11.1e-25359.77CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV... [more]
F4HSQ25.6e-22152.77Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 S... [more]
A9JQS72.7e-15944.50Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1[more]
Q8L7D24.1e-14743.08Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... [more]
Q9ZSP52.7e-14641.33Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LJ710.0e+0094.79Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G873800 PE=3 SV=1[more]
A0A6J1FFB50.0e+0085.05CO(2)-response secreted protease OS=Cucurbita moschata OX=3662 GN=LOC111443427 P... [more]
A0A6J1IHT10.0e+0084.79CO(2)-response secreted protease OS=Cucurbita maxima OX=3661 GN=LOC111476411 PE=... [more]
A0A6J1C9380.0e+0082.49CO(2)-response secreted protease OS=Momordica charantia OX=3673 GN=LOC111009346 ... [more]
A0A5D3E6D00.0e+0095.01CO(2)-response secreted protease isoform X2 OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
XP_004136374.10.0e+0094.79CO(2)-response secreted protease [Cucumis sativus] >KGN60051.1 hypothetical prot... [more]
XP_038896398.10.0e+0089.36CO(2)-response secreted protease [Benincasa hispida][more]
XP_022937005.10.0e+0085.05CO(2)-response secreted protease [Cucurbita moschata][more]
KAG6591258.10.0e+0085.05CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022975820.10.0e+0084.79CO(2)-response secreted protease [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G20160.18.0e-25559.77Subtilisin-like serine endopeptidase family protein [more]
AT1G20160.22.0e-25061.07Subtilisin-like serine endopeptidase family protein [more]
AT1G20150.14.0e-22252.77Subtilisin-like serine endopeptidase family protein [more]
AT5G59090.12.9e-14843.08subtilase 4.12 [more]
AT5G59090.21.1e-14742.95subtilase 4.12 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 137..156
score: 26.21
coord: 546..562
score: 52.07
coord: 208..221
score: 53.53
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 37..106
e-value: 1.5E-11
score: 44.8
NoneNo IPR availableGENE3D2.60.40.2310coord: 633..768
e-value: 8.3E-36
score: 124.6
NoneNo IPR availableGENE3D3.50.30.30coord: 341..486
e-value: 4.3E-178
score: 594.8
NoneNo IPR availablePANTHERPTHR10795:SF647SUBTILISIN-LIKE PROTEASE SBT5.1coord: 54..766
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 110..616
score: 27.131884
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 347..479
e-value: 3.13803E-24
score: 96.7139
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 667..765
e-value: 3.2E-28
score: 97.8
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 139..629
e-value: 4.3E-178
score: 594.8
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 138..620
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 21..106
e-value: 1.2E-18
score: 69.3
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 140..586
e-value: 9.6E-45
score: 153.0
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 54..766
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 547..557
IPR022398Peptidase S8, subtilisin, His-active sitePROSITEPS00137SUBTILASE_HIScoord: 212..222
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 103..583
e-value: 1.85088E-134
score: 398.51

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0012342.1PI0012342.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
cellular_component GO:0016020 membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity