Homology
BLAST of PI0011268 vs. ExPASy Swiss-Prot
Match:
F4HNU6 (Nardilysin-like OS=Arabidopsis thaliana OX=3702 GN=At1g06900 PE=2 SV=1)
HSP 1 Score: 696.4 bits (1796), Expect = 2.6e-199
Identity = 349/566 (61.66%), Postives = 432/566 (76.33%), Query Frame = 0
Query: 2 ESMEREVLAVDSD-------QSCHVNRSFFISSFLAME------GNKKSLVDATEKGINL 61
E+MEREVLAVDS+ +C + + +S GNKKSL A E G++L
Sbjct: 198 EAMEREVLAVDSEFNQALQNDACRLQQLQCYTSAKGHPFNRFAWGNKKSLSGAMENGVDL 257
Query: 62 REQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQS 121
RE I+KL+ +YYHGGLMKL VIGGESLD+LESWV+ELFG+VK G + +P + PIW+
Sbjct: 258 RECIVKLYKEYYHGGLMKLVVIGGESLDMLESWVVELFGDVKNGSKIRPTLEAEGPIWKG 317
Query: 122 GKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWATS 181
GKLY+LEAV+DVHIL L WTLP L++ Y+KKPE+Y+AHLL HEG+GSLH FLKAKGWATS
Sbjct: 318 GKLYRLEAVKDVHILDLTWTLPPLRSAYVKKPEDYLAHLLGHEGRGSLHSFLKAKGWATS 377
Query: 182 LSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFREL 241
LSA V DG++RSS+AY+F +SI+LTDSG EKIY+IIGY+YQ LKLLR VSPQEWIF+EL
Sbjct: 378 LSAGVGDDGINRSSLAYVFGMSIHLTDSGLEKIYDIIGYIYQYLKLLRDVSPQEWIFKEL 437
Query: 242 QDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTPE 301
QDIGNM+FRFAEE+P +DYAA+L+ N+ YP EHVIYG+Y Y+ WD L+ ++GFFTP+
Sbjct: 438 QDIGNMDFRFAEEQPADDYAAELSENMLAYPVEHVIYGDYVYQTWDPKLIEDLMGFFTPQ 497
Query: 302 NMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFIP 361
NMRID+ SK K E+F+ EPWFGS Y +D+P SLM+ W +P E+D SL+LPSKN+FIP
Sbjct: 498 NMRIDVVSKSI-KSEEFQQEPWFGSSYIEEDVPLSLMESWSNPSEVDNSLHLPSKNQFIP 557
Query: 362 SDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSVK 421
DFSIRA D +S PRCI+DEP MK WYKLD +FKVPRA TYFRINL GAY+SVK
Sbjct: 558 CDFSIRAINSDVDPKSQSPPRCIIDEPFMKFWYKLDETFKVPRANTYFRINLKGAYASVK 617
Query: 422 NNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLAT 481
N LLTEL+I LLKD+LN II+QA+IA L TS+++ DKLELKV+GFN+K+P LLS++LA
Sbjct: 618 NCLLTELYINLLKDELNEIIYQASIAKLETSLSMYGDKLELKVYGFNEKIPALLSKILAI 677
Query: 482 VTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDVL 541
+FMP+ +RF KVIKEN ER RN+ MKP H YLR ++L KR YD+DE++ VL
Sbjct: 678 AKSFMPNLERF-----KVIKENMERGFRNTNMKPLNHSTYLRLQLLCKRIYDSDEKLSVL 737
Query: 542 NDLSFVDLKAHIPELLPS-FITDSCH 554
NDLS DL + IPEL FI CH
Sbjct: 738 NDLSLDDLNSFIPELRSQIFIEALCH 757
BLAST of PI0011268 vs. ExPASy Swiss-Prot
Match:
P14735 (Insulin-degrading enzyme OS=Homo sapiens OX=9606 GN=IDE PE=1 SV=4)
HSP 1 Score: 292.4 bits (747), Expect = 1.1e-77
Identity = 189/563 (33.57%), Postives = 299/563 (53.11%), Query Frame = 0
Query: 5 EREVLAVDSDQSCHV---------------NRSFFISSFLAMEGNKKSL-VDATEKGINL 64
+REV AVDS+ +V N S F GNK +L ++GI++
Sbjct: 180 DREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKF--GTGNKYTLETRPNQEGIDV 239
Query: 65 REQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQS 124
R+++LK YY LM + V+G ESLD L + V++LF EV+ P F + P +
Sbjct: 240 RQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFP-EHPFQEE 299
Query: 125 --GKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWA 184
+LYK+ ++D+ L + + +P LQ Y P +Y+ HL+ HEG GSL LK+KGW
Sbjct: 300 HLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWV 359
Query: 185 TSLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFR 244
+L +K+G R M F+++++LT+ G + +II +++Q ++ LR PQEW+F+
Sbjct: 360 NTLVGG-QKEGA-RGFM--FFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQ 419
Query: 245 ELQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFT 304
E +D+ + FRF ++E Y +K+A L YP E V+ Y + + DL+ ++
Sbjct: 420 ECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLR 479
Query: 305 PENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEF 364
PEN+R+ I SK F D + E W+G+ Y + IP ++ W++ +++ LP+KNEF
Sbjct: 480 PENVRVAIVSKSFEGKTD-RTEEWYGTQYKQEAIPDEVIKKWQN-ADLNGKFKLPTKNEF 539
Query: 365 IPSDFSIRACKVCNDLPLESS----PRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSG 424
IP++F I LPLE P I D + KLW+K D+ F +P+AC F
Sbjct: 540 IPTNFEI--------LPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPF 599
Query: 425 AYSSVKNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLL 484
AY + + L++ELLKD LN + A +A L + + L V G+NDK P LL
Sbjct: 600 AYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILL 659
Query: 485 SEVLATVTTFMPSEDRFKVIFIKVIKENFERVLRN-STMKPGIHLLYLRPKVLYKRFYDA 544
+++ + TF E RF ++IKE + R L N +P H +Y ++ + +
Sbjct: 660 KKIIEKMATFEIDEKRF-----EIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTK 719
BLAST of PI0011268 vs. ExPASy Swiss-Prot
Match:
Q24K02 (Insulin-degrading enzyme OS=Bos taurus OX=9913 GN=IDE PE=2 SV=1)
HSP 1 Score: 289.3 bits (739), Expect = 9.4e-77
Identity = 188/563 (33.39%), Postives = 297/563 (52.75%), Query Frame = 0
Query: 5 EREVLAVDSDQSCHV---------------NRSFFISSFLAMEGNKKSL-VDATEKGINL 64
+REV AVDS+ +V N S F GNK +L ++GI++
Sbjct: 180 DREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKF--GTGNKYTLETRPNQEGIDV 239
Query: 65 REQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQS 124
R+++LK YY LM + V+G ESLD L + V++LF EV+ P F + P +
Sbjct: 240 RQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFP-EHPFQEE 299
Query: 125 --GKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWA 184
+LYK+ ++D+ L + + +P LQ Y P +Y+ HL+ HEG GSL LK+KGW
Sbjct: 300 HLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWV 359
Query: 185 TSLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFR 244
+L +K+G R M F+++++LT+ G + +II +++Q ++ LR PQEW+F+
Sbjct: 360 NTLVGG-QKEGA-RGFM--FFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQ 419
Query: 245 ELQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFT 304
E +D+ + FRF ++E Y +K+A L YP E V+ Y + + DL+ ++
Sbjct: 420 ECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLR 479
Query: 305 PENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEF 364
PEN+R+ I SK F D + E W+G+ Y + IP ++ W++ +++ LP KNEF
Sbjct: 480 PENVRVAIVSKSFEGKTD-RTEEWYGTQYKQEAIPDEVIKKWQN-ADLNGKFKLPMKNEF 539
Query: 365 IPSDFSIRACKVCNDLPLESS----PRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSG 424
IP++F I L LE P I D + KLW+K D+ F +P+AC F
Sbjct: 540 IPTNFEI--------LSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPF 599
Query: 425 AYSSVKNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLL 484
AY + + L++ELLKD LN + A +A L + + L V G+NDK P LL
Sbjct: 600 AYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILL 659
Query: 485 SEVLATVTTFMPSEDRFKVIFIKVIKENFERVLRN-STMKPGIHLLYLRPKVLYKRFYDA 544
+++ + TF E RF ++IKE + R L N +P H +Y ++ + +
Sbjct: 660 KKIIEKMATFEIDEKRF-----EIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTK 719
BLAST of PI0011268 vs. ExPASy Swiss-Prot
Match:
P35559 (Insulin-degrading enzyme OS=Rattus norvegicus OX=10116 GN=Ide PE=1 SV=1)
HSP 1 Score: 288.9 bits (738), Expect = 1.2e-76
Identity = 185/559 (33.09%), Postives = 296/559 (52.95%), Query Frame = 0
Query: 5 EREVLAVDSDQSCHV---------------NRSFFISSFLAMEGNKKSL-VDATEKGINL 64
+REV AVDS+ +V N S F GNK +L ++GI++
Sbjct: 180 DREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKF--GTGNKYTLETRPNQEGIDV 239
Query: 65 REQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQS 124
RE++LK YY LM + V+G ESLD L + V++LF EV+ P F + P +
Sbjct: 240 REELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFP-EHPFQEE 299
Query: 125 --GKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWA 184
+LYK+ ++D+ L + + +P LQ Y P +Y+ HL+ HEG GSL LK+KGW
Sbjct: 300 HLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWV 359
Query: 185 TSLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFR 244
+L +K+G R M F+++++LT+ G + +II +++Q ++ LR PQEW+F+
Sbjct: 360 NTLVGG-QKEGA-RGFM--FFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQ 419
Query: 245 ELQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFT 304
E +D+ + FRF ++E Y +K+A L YP V+ Y + + DL++ ++
Sbjct: 420 ECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMVLDKLR 479
Query: 305 PENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEF 364
PEN+R+ I SK F D + E W+G+ Y + IP ++ W++ +++ LP+KNEF
Sbjct: 480 PENVRVAIVSKSFEGKTD-RTEQWYGTQYKQEAIPEDVIQKWQN-ADLNGKFKLPTKNEF 539
Query: 365 IPSDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSS 424
IP++F I A + P I D + KLW+K D+ F +P+AC F AY
Sbjct: 540 IPTNFEILAL----EKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVD 599
Query: 425 VKNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVL 484
+ + L++ELLKD LN + A +A L + + L V G+NDK P LL ++
Sbjct: 600 PLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIT 659
Query: 485 ATVTTFMPSEDRFKVIFIKVIKENFERVLRN-STMKPGIHLLYLRPKVLYKRFYDADERI 544
+ TF + RF ++IKE + R L N +P H +Y ++ + + DE
Sbjct: 660 EKMATFEIDKKRF-----EIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELK 719
BLAST of PI0011268 vs. ExPASy Swiss-Prot
Match:
Q9JHR7 (Insulin-degrading enzyme OS=Mus musculus OX=10090 GN=Ide PE=1 SV=1)
HSP 1 Score: 283.5 bits (724), Expect = 5.2e-75
Identity = 186/563 (33.04%), Postives = 295/563 (52.40%), Query Frame = 0
Query: 5 EREVLAVDSDQSCHV---------------NRSFFISSFLAMEGNKKSL-VDATEKGINL 64
+REV AVDS+ +V N S F GNK +L ++GI++
Sbjct: 180 DREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKF--GTGNKYTLETRPNQEGIDV 239
Query: 65 REQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQS 124
RE++LK YY LM + V+G ESLD L + V++LF EV+ P F + P +
Sbjct: 240 REELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVENKNVPLPEFP-EHPFQEE 299
Query: 125 --GKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWA 184
+LYK+ ++D+ L + + +P LQ Y P Y+ HL+ HEG GSL LK+KGW
Sbjct: 300 HLRQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGYYLGHLIGHEGPGSLLSELKSKGWV 359
Query: 185 TSLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFR 244
+L +K+G R M F+++++LT+ G + +II +++Q ++ LR PQEW+F+
Sbjct: 360 NTLVGG-QKEGA-RGFM--FFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQ 419
Query: 245 ELQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFT 304
E +D+ + FRF ++E Y +K+A L YP V+ Y + + DL++ ++
Sbjct: 420 ECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFRPDLIDMVLDKLR 479
Query: 305 PENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEF 364
PEN+R+ I SK F D + E W+G+ Y + IP ++ W++ +++ LP+KNEF
Sbjct: 480 PENVRVAIVSKSFEGKTD-RTEQWYGTQYKQEAIPEDVIQKWQN-ADLNGKFKLPTKNEF 539
Query: 365 IPSDFSIRACKVCNDLPLESS----PRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSG 424
IP++F I L LE P I D + KLW+K D+ F +P+AC F
Sbjct: 540 IPTNFEI--------LSLEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPF 599
Query: 425 AYSSVKNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLL 484
AY + + L++ELLKD LN + A +A L + + L V +NDK P LL
Sbjct: 600 AYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKRYNDKQPILL 659
Query: 485 SEVLATVTTFMPSEDRFKVIFIKVIKENFERVLRN-STMKPGIHLLYLRPKVLYKRFYDA 544
++ + TF + RF ++IKE + R L N +P H +Y ++ + +
Sbjct: 660 KKITEKMATFEIDKKRF-----EIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTK 719
BLAST of PI0011268 vs. ExPASy TrEMBL
Match:
A0A5D3DT90 (Nardilysin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold260G00220 PE=3 SV=1)
HSP 1 Score: 875.2 bits (2260), Expect = 1.5e-250
Identity = 444/567 (78.31%), Postives = 481/567 (84.83%), Query Frame = 0
Query: 1 MESMEREVLAVDSD-------QSCHVNRSFFISSFLA------MEGNKKSLVDATEKGIN 60
+E+MEREVLAVDS+ C + + +S GNKKSLVDA EKGIN
Sbjct: 288 IEAMEREVLAVDSEFNQVLQNDVCRLQQLQCYTSVPGHPFNRFFWGNKKSLVDAMEKGIN 347
Query: 61 LREQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQ 120
LREQILKLF DYYHGGLMKLTVIGGE LD+LESWVLELF ++KKGVQAKP+FTVKDPIWQ
Sbjct: 348 LREQILKLFRDYYHGGLMKLTVIGGEPLDMLESWVLELFVDLKKGVQAKPKFTVKDPIWQ 407
Query: 121 SGKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWAT 180
SGKLYKLEAV+DVHIL LAWTLPCLQ+NYLKKPE+YIAHLL HEGKGSLHFFLKAKGWAT
Sbjct: 408 SGKLYKLEAVEDVHILDLAWTLPCLQHNYLKKPEDYIAHLLGHEGKGSLHFFLKAKGWAT 467
Query: 181 SLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRE 240
SLSA V DGM RSSMAY+F +SI LTDSGKEKI+EIIGYVYQ LKLLRQ+SPQEWIFRE
Sbjct: 468 SLSAGVGDDGMCRSSMAYVFGMSIYLTDSGKEKIFEIIGYVYQYLKLLRQISPQEWIFRE 527
Query: 241 LQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTP 300
LQDIGNMEFRFAEE+PQ+DYAA+LA NLS YPAEHVIYG Y +KIWDEDLV HIIGFFTP
Sbjct: 528 LQDIGNMEFRFAEEQPQDDYAAELAENLSFYPAEHVIYGEYVHKIWDEDLVKHIIGFFTP 587
Query: 301 ENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFI 360
ENMR+DI SK FSKLEDFKIEPWFGSHYSVDDI SLMDLWRDPPEID SLYLP+KNEFI
Sbjct: 588 ENMRVDIVSKSFSKLEDFKIEPWFGSHYSVDDIAPSLMDLWRDPPEIDASLYLPAKNEFI 647
Query: 361 PSDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSV 420
P DFSIRA KVCNDL LESSPRCILDEPLMK WYKLDNSFK+PRA TYFRINLSG YSSV
Sbjct: 648 PRDFSIRASKVCNDLLLESSPRCILDEPLMKFWYKLDNSFKLPRANTYFRINLSGGYSSV 707
Query: 421 KNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLA 480
KN LLTELF+ LLKDKLN II+QA+IA L TS+A+ DKLELKVFGFNDKLPNLLS++LA
Sbjct: 708 KNCLLTELFVHLLKDKLNDIIYQASIAKLETSVAISGDKLELKVFGFNDKLPNLLSKLLA 767
Query: 481 TVTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDV 540
T TFMPSEDRF KVIKE ER LRN+ MKP H YLR +VL +RFYDADE+ DV
Sbjct: 768 TAKTFMPSEDRF-----KVIKEKMERNLRNTNMKPRSHSSYLRLQVLCERFYDADEKSDV 827
Query: 541 LNDLSFVDLKAHIPELLPS-FITDSCH 554
LNDLSFVDLKAHIPE+L +I CH
Sbjct: 828 LNDLSFVDLKAHIPEVLSQLYIEGLCH 849
BLAST of PI0011268 vs. ExPASy TrEMBL
Match:
A0A5A7SU38 (Nardilysin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold261G00330 PE=3 SV=1)
HSP 1 Score: 875.2 bits (2260), Expect = 1.5e-250
Identity = 444/567 (78.31%), Postives = 481/567 (84.83%), Query Frame = 0
Query: 1 MESMEREVLAVDSD-------QSCHVNRSFFISSFLA------MEGNKKSLVDATEKGIN 60
+E+MEREVLAVDS+ C + + +S GNKKSLVDA EKGIN
Sbjct: 191 IEAMEREVLAVDSEFNQVLQNDVCRLQQLQCYTSVPGHPFNRFFWGNKKSLVDAMEKGIN 250
Query: 61 LREQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQ 120
LREQILKLF DYYHGGLMKLTVIGGE LD+LESWVLELF ++KKGVQAKP+FTVKDPIWQ
Sbjct: 251 LREQILKLFRDYYHGGLMKLTVIGGEPLDMLESWVLELFVDLKKGVQAKPKFTVKDPIWQ 310
Query: 121 SGKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWAT 180
SGKLYKLEAV+DVHIL LAWTLPCLQ+NYLKKPE+YIAHLL HEGKGSLHFFLKAKGWAT
Sbjct: 311 SGKLYKLEAVEDVHILDLAWTLPCLQHNYLKKPEDYIAHLLGHEGKGSLHFFLKAKGWAT 370
Query: 181 SLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRE 240
SLSA V DGM RSSMAY+F +SI LTDSGKEKI+EIIGYVYQ LKLLRQ+SPQEWIFRE
Sbjct: 371 SLSAGVGDDGMCRSSMAYVFGMSIYLTDSGKEKIFEIIGYVYQYLKLLRQISPQEWIFRE 430
Query: 241 LQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTP 300
LQDIGNMEFRFAEE+PQ+DYAA+LA NLS YPAEHVIYG Y +KIWDEDLV HIIGFFTP
Sbjct: 431 LQDIGNMEFRFAEEQPQDDYAAELAENLSFYPAEHVIYGEYVHKIWDEDLVKHIIGFFTP 490
Query: 301 ENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFI 360
ENMR+DI SK FSKLEDFKIEPWFGSHYSVDDI SLMDLWRDPPEID SLYLP+KNEFI
Sbjct: 491 ENMRVDIVSKSFSKLEDFKIEPWFGSHYSVDDIAPSLMDLWRDPPEIDASLYLPAKNEFI 550
Query: 361 PSDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSV 420
P DFSIRA KVCNDL LESSPRCILDEPLMK WYKLDNSFK+PRA TYFRINLSG YSSV
Sbjct: 551 PRDFSIRASKVCNDLLLESSPRCILDEPLMKFWYKLDNSFKLPRANTYFRINLSGGYSSV 610
Query: 421 KNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLA 480
KN LLTELF+ LLKDKLN II+QA+IA L TS+A+ DKLELKVFGFNDKLPNLLS++LA
Sbjct: 611 KNCLLTELFVHLLKDKLNDIIYQASIAKLETSVAISGDKLELKVFGFNDKLPNLLSKLLA 670
Query: 481 TVTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDV 540
T TFMPSEDRF KVIKE ER LRN+ MKP H YLR +VL +RFYDADE+ DV
Sbjct: 671 TAKTFMPSEDRF-----KVIKEKMERNLRNTNMKPRSHSSYLRLQVLCERFYDADEKSDV 730
Query: 541 LNDLSFVDLKAHIPELLPS-FITDSCH 554
LNDLSFVDLKAHIPE+L +I CH
Sbjct: 731 LNDLSFVDLKAHIPEVLSQLYIEGLCH 752
BLAST of PI0011268 vs. ExPASy TrEMBL
Match:
A0A1S3CNH0 (nardilysin-like OS=Cucumis melo OX=3656 GN=LOC103502959 PE=3 SV=1)
HSP 1 Score: 875.2 bits (2260), Expect = 1.5e-250
Identity = 444/567 (78.31%), Postives = 481/567 (84.83%), Query Frame = 0
Query: 1 MESMEREVLAVDSD-------QSCHVNRSFFISSFLA------MEGNKKSLVDATEKGIN 60
+E+MEREVLAVDS+ C + + +S GNKKSLVDA EKGIN
Sbjct: 191 IEAMEREVLAVDSEFNQVLQNDVCRLQQLQCYTSVPGHPFNRFFWGNKKSLVDAMEKGIN 250
Query: 61 LREQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQ 120
LREQILKLF DYYHGGLMKLTVIGGE LD+LESWVLELF ++KKGVQAKP+FTVKDPIWQ
Sbjct: 251 LREQILKLFRDYYHGGLMKLTVIGGEPLDMLESWVLELFVDLKKGVQAKPKFTVKDPIWQ 310
Query: 121 SGKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWAT 180
SGKLYKLEAV+DVHIL LAWTLPCLQ+NYLKKPE+YIAHLL HEGKGSLHFFLKAKGWAT
Sbjct: 311 SGKLYKLEAVEDVHILDLAWTLPCLQHNYLKKPEDYIAHLLGHEGKGSLHFFLKAKGWAT 370
Query: 181 SLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRE 240
SLSA V DGM RSSMAY+F +SI LTDSGKEKI+EIIGYVYQ LKLLRQ+SPQEWIFRE
Sbjct: 371 SLSAGVGDDGMCRSSMAYVFGMSIYLTDSGKEKIFEIIGYVYQYLKLLRQISPQEWIFRE 430
Query: 241 LQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTP 300
LQDIGNMEFRFAEE+PQ+DYAA+LA NLS YPAEHVIYG Y +KIWDEDLV HIIGFFTP
Sbjct: 431 LQDIGNMEFRFAEEQPQDDYAAELAENLSFYPAEHVIYGEYVHKIWDEDLVKHIIGFFTP 490
Query: 301 ENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFI 360
ENMR+DI SK FSKLEDFKIEPWFGSHYSVDDI SLMDLWRDPPEID SLYLP+KNEFI
Sbjct: 491 ENMRVDIVSKSFSKLEDFKIEPWFGSHYSVDDIAPSLMDLWRDPPEIDASLYLPAKNEFI 550
Query: 361 PSDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSV 420
P DFSIRA KVCNDL LESSPRCILDEPLMK WYKLDNSFK+PRA TYFRINLSG YSSV
Sbjct: 551 PRDFSIRASKVCNDLLLESSPRCILDEPLMKFWYKLDNSFKLPRANTYFRINLSGGYSSV 610
Query: 421 KNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLA 480
KN LLTELF+ LLKDKLN II+QA+IA L TS+A+ DKLELKVFGFNDKLPNLLS++LA
Sbjct: 611 KNCLLTELFVHLLKDKLNDIIYQASIAKLETSVAISGDKLELKVFGFNDKLPNLLSKLLA 670
Query: 481 TVTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDV 540
T TFMPSEDRF KVIKE ER LRN+ MKP H YLR +VL +RFYDADE+ DV
Sbjct: 671 TAKTFMPSEDRF-----KVIKEKMERNLRNTNMKPRSHSSYLRLQVLCERFYDADEKSDV 730
Query: 541 LNDLSFVDLKAHIPELLPS-FITDSCH 554
LNDLSFVDLKAHIPE+L +I CH
Sbjct: 731 LNDLSFVDLKAHIPEVLSQLYIEGLCH 752
BLAST of PI0011268 vs. ExPASy TrEMBL
Match:
A0A6J1FUR8 (nardilysin-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448618 PE=3 SV=1)
HSP 1 Score: 846.3 bits (2185), Expect = 7.4e-242
Identity = 427/567 (75.31%), Postives = 476/567 (83.95%), Query Frame = 0
Query: 1 MESMEREVLAVDSD-------QSCHVNRSFFISSFLA------MEGNKKSLVDATEKGIN 60
ME+MEREVLAVDS+ +C + + +S GNKKSLVDA EKGIN
Sbjct: 211 MEAMEREVLAVDSEFNQVLQNDACRLQQLQCYTSVPGHPFNRFFWGNKKSLVDAMEKGIN 270
Query: 61 LREQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQ 120
LREQILKLF DYYHGGLMKLTVIGGE LD+LESWVLELF +VKKGVQ KP FTVKDPIWQ
Sbjct: 271 LREQILKLFRDYYHGGLMKLTVIGGEPLDILESWVLELFDDVKKGVQVKPVFTVKDPIWQ 330
Query: 121 SGKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWAT 180
SGKLYKLEAV+DVHIL LAWTLPCLQ+NYLKKPE+YIAHLL HEGKGSLHFFLKAKGWAT
Sbjct: 331 SGKLYKLEAVEDVHILDLAWTLPCLQHNYLKKPEDYIAHLLGHEGKGSLHFFLKAKGWAT 390
Query: 181 SLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRE 240
SLSA V +GM+RSS+AY+F +SI LTDSG EKI+EIIGYVYQ LKLLRQVSPQEWIFRE
Sbjct: 391 SLSAGVGDEGMYRSSIAYVFGMSIYLTDSGLEKIFEIIGYVYQYLKLLRQVSPQEWIFRE 450
Query: 241 LQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTP 300
LQDIGNM+FRFAEE+PQ+DYAA+LA NL+ YP EHVI+G+Y ++IWD DLV HIIG FTP
Sbjct: 451 LQDIGNMDFRFAEEQPQDDYAAELAENLTFYPPEHVIFGDYVHQIWDVDLVKHIIGLFTP 510
Query: 301 ENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFI 360
ENMRIDI SK FSKLEDFK+EPWFGSHY+VDDI SLMDLWRDPPEID SL+LP+KNEFI
Sbjct: 511 ENMRIDIVSKSFSKLEDFKVEPWFGSHYTVDDIAPSLMDLWRDPPEIDASLHLPAKNEFI 570
Query: 361 PSDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSV 420
P DFSIRA +VCNDL ES PRCILDEPLMK WYKLD+SFK+PRA TYFRINLSG YSSV
Sbjct: 571 PCDFSIRASQVCNDLSCESCPRCILDEPLMKFWYKLDDSFKLPRANTYFRINLSGGYSSV 630
Query: 421 KNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLA 480
KN LLTELF+ LLKDKLN II+QA+IA L TS+A+ DKLELKVFGFNDKLPNLL+++L+
Sbjct: 631 KNCLLTELFVHLLKDKLNEIIYQASIAKLETSVAIIGDKLELKVFGFNDKLPNLLAKLLS 690
Query: 481 TVTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDV 540
T TFMPSEDRF KVIKEN ER LRN+ MKP H YLR +VL +RFYDADE+ +V
Sbjct: 691 TAKTFMPSEDRF-----KVIKENMERNLRNTNMKPRSHSSYLRLQVLCERFYDADEKSNV 750
Query: 541 LNDLSFVDLKAHIPELLPS-FITDSCH 554
LNDLSFV+LKAHIP LL +I CH
Sbjct: 751 LNDLSFVELKAHIPVLLSQLYIEGLCH 772
BLAST of PI0011268 vs. ExPASy TrEMBL
Match:
A0A6J1FXN2 (nardilysin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448618 PE=3 SV=1)
HSP 1 Score: 846.3 bits (2185), Expect = 7.4e-242
Identity = 427/567 (75.31%), Postives = 476/567 (83.95%), Query Frame = 0
Query: 1 MESMEREVLAVDSD-------QSCHVNRSFFISSFLA------MEGNKKSLVDATEKGIN 60
ME+MEREVLAVDS+ +C + + +S GNKKSLVDA EKGIN
Sbjct: 184 MEAMEREVLAVDSEFNQVLQNDACRLQQLQCYTSVPGHPFNRFFWGNKKSLVDAMEKGIN 243
Query: 61 LREQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQ 120
LREQILKLF DYYHGGLMKLTVIGGE LD+LESWVLELF +VKKGVQ KP FTVKDPIWQ
Sbjct: 244 LREQILKLFRDYYHGGLMKLTVIGGEPLDILESWVLELFDDVKKGVQVKPVFTVKDPIWQ 303
Query: 121 SGKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWAT 180
SGKLYKLEAV+DVHIL LAWTLPCLQ+NYLKKPE+YIAHLL HEGKGSLHFFLKAKGWAT
Sbjct: 304 SGKLYKLEAVEDVHILDLAWTLPCLQHNYLKKPEDYIAHLLGHEGKGSLHFFLKAKGWAT 363
Query: 181 SLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRE 240
SLSA V +GM+RSS+AY+F +SI LTDSG EKI+EIIGYVYQ LKLLRQVSPQEWIFRE
Sbjct: 364 SLSAGVGDEGMYRSSIAYVFGMSIYLTDSGLEKIFEIIGYVYQYLKLLRQVSPQEWIFRE 423
Query: 241 LQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTP 300
LQDIGNM+FRFAEE+PQ+DYAA+LA NL+ YP EHVI+G+Y ++IWD DLV HIIG FTP
Sbjct: 424 LQDIGNMDFRFAEEQPQDDYAAELAENLTFYPPEHVIFGDYVHQIWDVDLVKHIIGLFTP 483
Query: 301 ENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFI 360
ENMRIDI SK FSKLEDFK+EPWFGSHY+VDDI SLMDLWRDPPEID SL+LP+KNEFI
Sbjct: 484 ENMRIDIVSKSFSKLEDFKVEPWFGSHYTVDDIAPSLMDLWRDPPEIDASLHLPAKNEFI 543
Query: 361 PSDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSV 420
P DFSIRA +VCNDL ES PRCILDEPLMK WYKLD+SFK+PRA TYFRINLSG YSSV
Sbjct: 544 PCDFSIRASQVCNDLSCESCPRCILDEPLMKFWYKLDDSFKLPRANTYFRINLSGGYSSV 603
Query: 421 KNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLA 480
KN LLTELF+ LLKDKLN II+QA+IA L TS+A+ DKLELKVFGFNDKLPNLL+++L+
Sbjct: 604 KNCLLTELFVHLLKDKLNEIIYQASIAKLETSVAIIGDKLELKVFGFNDKLPNLLAKLLS 663
Query: 481 TVTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDV 540
T TFMPSEDRF KVIKEN ER LRN+ MKP H YLR +VL +RFYDADE+ +V
Sbjct: 664 TAKTFMPSEDRF-----KVIKENMERNLRNTNMKPRSHSSYLRLQVLCERFYDADEKSNV 723
Query: 541 LNDLSFVDLKAHIPELLPS-FITDSCH 554
LNDLSFV+LKAHIP LL +I CH
Sbjct: 724 LNDLSFVELKAHIPVLLSQLYIEGLCH 745
BLAST of PI0011268 vs. NCBI nr
Match:
XP_038901221.1 (nardilysin-like [Benincasa hispida])
HSP 1 Score: 877.9 bits (2267), Expect = 4.7e-251
Identity = 443/566 (78.27%), Postives = 485/566 (85.69%), Query Frame = 0
Query: 2 ESMEREVLAVDSD-------QSCHVNRSFFISSFLA------MEGNKKSLVDATEKGINL 61
E+MEREVLAVDS+ +C + + SS GNKKSLVDA EKGINL
Sbjct: 223 EAMEREVLAVDSEFNQVLQNDACRLQQLQCYSSVPGHPFNRFFWGNKKSLVDAMEKGINL 282
Query: 62 REQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQS 121
REQILKLF DYYHGGLMKLTVIGGE LD+LE+WVLELFG+VKKGVQAKPRFTVKDPIWQS
Sbjct: 283 REQILKLFRDYYHGGLMKLTVIGGEPLDILENWVLELFGKVKKGVQAKPRFTVKDPIWQS 342
Query: 122 GKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWATS 181
GKLYKLEAV+DVHIL LAWTLPCL+++YLKKPE+YIAHLL HEGKGSLHFFLKAKGWATS
Sbjct: 343 GKLYKLEAVEDVHILDLAWTLPCLKHDYLKKPEDYIAHLLGHEGKGSLHFFLKAKGWATS 402
Query: 182 LSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFREL 241
LSA V +GM+RSSMAY+F +SI LTDSGKEKI+EIIGYVYQ LKLLRQVSPQEWIF+EL
Sbjct: 403 LSAGVGDEGMYRSSMAYVFGMSIYLTDSGKEKIFEIIGYVYQYLKLLRQVSPQEWIFKEL 462
Query: 242 QDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTPE 301
QDIGNMEFRFAEE+PQ+DYAA+LA NLS YPAEHVIYG+Y +KIW+EDLV HIIGFFTPE
Sbjct: 463 QDIGNMEFRFAEEQPQDDYAAELAENLSFYPAEHVIYGDYVHKIWEEDLVKHIIGFFTPE 522
Query: 302 NMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFIP 361
NMR+DI SK FSKLEDFKIEPWFGSHYSVDDIP SLMDLWRDPPEID SL+LP++NEFIP
Sbjct: 523 NMRVDIVSKSFSKLEDFKIEPWFGSHYSVDDIPPSLMDLWRDPPEIDASLHLPAQNEFIP 582
Query: 362 SDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSVK 421
DFSIRA KVCNDL LESSPRCILDEPLMK WYKLDNSFK+PRA TYFRINLSG YSSVK
Sbjct: 583 CDFSIRASKVCNDLLLESSPRCILDEPLMKFWYKLDNSFKLPRANTYFRINLSGGYSSVK 642
Query: 422 NNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLAT 481
N LLTELF+ LLKDKLN II+QA+IA L TS+A+ DKLELKVFGFNDKLPNLLS++L T
Sbjct: 643 NCLLTELFVHLLKDKLNEIIYQASIAKLETSVAIFGDKLELKVFGFNDKLPNLLSKLLGT 702
Query: 482 VTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDVL 541
TFMPSEDRF KVIKEN ER LRN+ MKP H YLR +VL +RFYDADE+ DVL
Sbjct: 703 AKTFMPSEDRF-----KVIKENMERNLRNTNMKPRSHSSYLRLQVLCERFYDADEKSDVL 762
Query: 542 NDLSFVDLKAHIPELLPS-FITDSCH 554
NDLSFVDLKAHIPELL +I CH
Sbjct: 763 NDLSFVDLKAHIPELLSQLYIEGLCH 783
BLAST of PI0011268 vs. NCBI nr
Match:
XP_008465304.1 (PREDICTED: nardilysin-like [Cucumis melo] >KAA0033496.1 nardilysin-like [Cucumis melo var. makuwa])
HSP 1 Score: 875.2 bits (2260), Expect = 3.1e-250
Identity = 444/567 (78.31%), Postives = 481/567 (84.83%), Query Frame = 0
Query: 1 MESMEREVLAVDSD-------QSCHVNRSFFISSFLA------MEGNKKSLVDATEKGIN 60
+E+MEREVLAVDS+ C + + +S GNKKSLVDA EKGIN
Sbjct: 191 IEAMEREVLAVDSEFNQVLQNDVCRLQQLQCYTSVPGHPFNRFFWGNKKSLVDAMEKGIN 250
Query: 61 LREQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQ 120
LREQILKLF DYYHGGLMKLTVIGGE LD+LESWVLELF ++KKGVQAKP+FTVKDPIWQ
Sbjct: 251 LREQILKLFRDYYHGGLMKLTVIGGEPLDMLESWVLELFVDLKKGVQAKPKFTVKDPIWQ 310
Query: 121 SGKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWAT 180
SGKLYKLEAV+DVHIL LAWTLPCLQ+NYLKKPE+YIAHLL HEGKGSLHFFLKAKGWAT
Sbjct: 311 SGKLYKLEAVEDVHILDLAWTLPCLQHNYLKKPEDYIAHLLGHEGKGSLHFFLKAKGWAT 370
Query: 181 SLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRE 240
SLSA V DGM RSSMAY+F +SI LTDSGKEKI+EIIGYVYQ LKLLRQ+SPQEWIFRE
Sbjct: 371 SLSAGVGDDGMCRSSMAYVFGMSIYLTDSGKEKIFEIIGYVYQYLKLLRQISPQEWIFRE 430
Query: 241 LQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTP 300
LQDIGNMEFRFAEE+PQ+DYAA+LA NLS YPAEHVIYG Y +KIWDEDLV HIIGFFTP
Sbjct: 431 LQDIGNMEFRFAEEQPQDDYAAELAENLSFYPAEHVIYGEYVHKIWDEDLVKHIIGFFTP 490
Query: 301 ENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFI 360
ENMR+DI SK FSKLEDFKIEPWFGSHYSVDDI SLMDLWRDPPEID SLYLP+KNEFI
Sbjct: 491 ENMRVDIVSKSFSKLEDFKIEPWFGSHYSVDDIAPSLMDLWRDPPEIDASLYLPAKNEFI 550
Query: 361 PSDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSV 420
P DFSIRA KVCNDL LESSPRCILDEPLMK WYKLDNSFK+PRA TYFRINLSG YSSV
Sbjct: 551 PRDFSIRASKVCNDLLLESSPRCILDEPLMKFWYKLDNSFKLPRANTYFRINLSGGYSSV 610
Query: 421 KNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLA 480
KN LLTELF+ LLKDKLN II+QA+IA L TS+A+ DKLELKVFGFNDKLPNLLS++LA
Sbjct: 611 KNCLLTELFVHLLKDKLNDIIYQASIAKLETSVAISGDKLELKVFGFNDKLPNLLSKLLA 670
Query: 481 TVTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDV 540
T TFMPSEDRF KVIKE ER LRN+ MKP H YLR +VL +RFYDADE+ DV
Sbjct: 671 TAKTFMPSEDRF-----KVIKEKMERNLRNTNMKPRSHSSYLRLQVLCERFYDADEKSDV 730
Query: 541 LNDLSFVDLKAHIPELLPS-FITDSCH 554
LNDLSFVDLKAHIPE+L +I CH
Sbjct: 731 LNDLSFVDLKAHIPEVLSQLYIEGLCH 752
BLAST of PI0011268 vs. NCBI nr
Match:
TYK26833.1 (nardilysin-like [Cucumis melo var. makuwa])
HSP 1 Score: 875.2 bits (2260), Expect = 3.1e-250
Identity = 444/567 (78.31%), Postives = 481/567 (84.83%), Query Frame = 0
Query: 1 MESMEREVLAVDSD-------QSCHVNRSFFISSFLA------MEGNKKSLVDATEKGIN 60
+E+MEREVLAVDS+ C + + +S GNKKSLVDA EKGIN
Sbjct: 288 IEAMEREVLAVDSEFNQVLQNDVCRLQQLQCYTSVPGHPFNRFFWGNKKSLVDAMEKGIN 347
Query: 61 LREQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQ 120
LREQILKLF DYYHGGLMKLTVIGGE LD+LESWVLELF ++KKGVQAKP+FTVKDPIWQ
Sbjct: 348 LREQILKLFRDYYHGGLMKLTVIGGEPLDMLESWVLELFVDLKKGVQAKPKFTVKDPIWQ 407
Query: 121 SGKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWAT 180
SGKLYKLEAV+DVHIL LAWTLPCLQ+NYLKKPE+YIAHLL HEGKGSLHFFLKAKGWAT
Sbjct: 408 SGKLYKLEAVEDVHILDLAWTLPCLQHNYLKKPEDYIAHLLGHEGKGSLHFFLKAKGWAT 467
Query: 181 SLSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRE 240
SLSA V DGM RSSMAY+F +SI LTDSGKEKI+EIIGYVYQ LKLLRQ+SPQEWIFRE
Sbjct: 468 SLSAGVGDDGMCRSSMAYVFGMSIYLTDSGKEKIFEIIGYVYQYLKLLRQISPQEWIFRE 527
Query: 241 LQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTP 300
LQDIGNMEFRFAEE+PQ+DYAA+LA NLS YPAEHVIYG Y +KIWDEDLV HIIGFFTP
Sbjct: 528 LQDIGNMEFRFAEEQPQDDYAAELAENLSFYPAEHVIYGEYVHKIWDEDLVKHIIGFFTP 587
Query: 301 ENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFI 360
ENMR+DI SK FSKLEDFKIEPWFGSHYSVDDI SLMDLWRDPPEID SLYLP+KNEFI
Sbjct: 588 ENMRVDIVSKSFSKLEDFKIEPWFGSHYSVDDIAPSLMDLWRDPPEIDASLYLPAKNEFI 647
Query: 361 PSDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSV 420
P DFSIRA KVCNDL LESSPRCILDEPLMK WYKLDNSFK+PRA TYFRINLSG YSSV
Sbjct: 648 PRDFSIRASKVCNDLLLESSPRCILDEPLMKFWYKLDNSFKLPRANTYFRINLSGGYSSV 707
Query: 421 KNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLA 480
KN LLTELF+ LLKDKLN II+QA+IA L TS+A+ DKLELKVFGFNDKLPNLLS++LA
Sbjct: 708 KNCLLTELFVHLLKDKLNDIIYQASIAKLETSVAISGDKLELKVFGFNDKLPNLLSKLLA 767
Query: 481 TVTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDV 540
T TFMPSEDRF KVIKE ER LRN+ MKP H YLR +VL +RFYDADE+ DV
Sbjct: 768 TAKTFMPSEDRF-----KVIKEKMERNLRNTNMKPRSHSSYLRLQVLCERFYDADEKSDV 827
Query: 541 LNDLSFVDLKAHIPELLPS-FITDSCH 554
LNDLSFVDLKAHIPE+L +I CH
Sbjct: 828 LNDLSFVDLKAHIPEVLSQLYIEGLCH 849
BLAST of PI0011268 vs. NCBI nr
Match:
XP_004150605.1 (nardilysin-like isoform X1 [Cucumis sativus] >KAE8647021.1 hypothetical protein Csa_017005 [Cucumis sativus])
HSP 1 Score: 869.0 bits (2244), Expect = 2.2e-248
Identity = 439/566 (77.56%), Postives = 478/566 (84.45%), Query Frame = 0
Query: 2 ESMEREVLAVDS-------DQSCHVNRSFFISSFLA------MEGNKKSLVDATEKGINL 61
E+MEREVLAVDS D SC + + +S GNKKSLVDA EKGINL
Sbjct: 193 EAMEREVLAVDSEFNQVLQDDSCRLQQLQCYTSVPGHPFNRFFWGNKKSLVDAMEKGINL 252
Query: 62 REQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQS 121
R+QILKLF DYYHGGLMKLTVIGGE LDVLESWVLELFG+VKKGVQAKP+FTVKDPIWQS
Sbjct: 253 RDQILKLFSDYYHGGLMKLTVIGGEPLDVLESWVLELFGDVKKGVQAKPKFTVKDPIWQS 312
Query: 122 GKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWATS 181
GKLYKLEAV+DVHIL LAWTLPCLQ+NYLKKPE+Y+AHLL HEG GSLHF LKAKGWATS
Sbjct: 313 GKLYKLEAVEDVHILDLAWTLPCLQHNYLKKPEDYVAHLLGHEGNGSLHFSLKAKGWATS 372
Query: 182 LSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFREL 241
LSA V +GM RSSMAY+F +SI LTDSGKEKI+EIIGYVYQ LKLLRQ+SPQEWIFREL
Sbjct: 373 LSAGVGDEGMCRSSMAYVFGMSIYLTDSGKEKIFEIIGYVYQYLKLLRQISPQEWIFREL 432
Query: 242 QDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTPE 301
QDIGNM+FRFAEE+PQ+DYAA+LA NLS YPAEHVIYG Y YKIWDEDLV HIIGFFTPE
Sbjct: 433 QDIGNMDFRFAEEQPQDDYAAELAENLSFYPAEHVIYGEYVYKIWDEDLVKHIIGFFTPE 492
Query: 302 NMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFIP 361
NMR+DI SK FSKLEDFKIEPWFGSHYSVDDI SLMDLWRDPPEID SL+LP+KN+FIP
Sbjct: 493 NMRVDIVSKSFSKLEDFKIEPWFGSHYSVDDIAPSLMDLWRDPPEIDASLHLPAKNQFIP 552
Query: 362 SDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSVK 421
DFSIRA KVCN+LPLE SP CILDEPLMK WYKLDNSFK+PRA TYF INLSG YSSVK
Sbjct: 553 CDFSIRASKVCNNLPLEYSPICILDEPLMKFWYKLDNSFKLPRANTYFHINLSGGYSSVK 612
Query: 422 NNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLAT 481
N LLTELF+ LLKDKLN II+QATIA L TS+A+ DKLELKVFGFNDKLPNLLS++LAT
Sbjct: 613 NYLLTELFVLLLKDKLNEIIYQATIAKLETSVAISGDKLELKVFGFNDKLPNLLSKLLAT 672
Query: 482 VTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDVL 541
TFMPSEDRF KVIKE ER L+N+ MKP H YLR +VL +RFYDADE+ DVL
Sbjct: 673 ARTFMPSEDRF-----KVIKEKMERNLKNTNMKPRSHSSYLRLQVLCERFYDADEKSDVL 732
Query: 542 NDLSFVDLKAHIPELLPS-FITDSCH 554
NDLSFVDLKAHIP+LL +I CH
Sbjct: 733 NDLSFVDLKAHIPKLLSQLYIEGLCH 753
BLAST of PI0011268 vs. NCBI nr
Match:
XP_031743090.1 (nardilysin-like isoform X2 [Cucumis sativus])
HSP 1 Score: 867.1 bits (2239), Expect = 8.4e-248
Identity = 438/564 (77.66%), Postives = 476/564 (84.40%), Query Frame = 0
Query: 4 MEREVLAVDS-------DQSCHVNRSFFISSFLA------MEGNKKSLVDATEKGINLRE 63
MEREVLAVDS D SC + + +S GNKKSLVDA EKGINLR+
Sbjct: 1 MEREVLAVDSEFNQVLQDDSCRLQQLQCYTSVPGHPFNRFFWGNKKSLVDAMEKGINLRD 60
Query: 64 QILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQSGK 123
QILKLF DYYHGGLMKLTVIGGE LDVLESWVLELFG+VKKGVQAKP+FTVKDPIWQSGK
Sbjct: 61 QILKLFSDYYHGGLMKLTVIGGEPLDVLESWVLELFGDVKKGVQAKPKFTVKDPIWQSGK 120
Query: 124 LYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWATSLS 183
LYKLEAV+DVHIL LAWTLPCLQ+NYLKKPE+Y+AHLL HEG GSLHF LKAKGWATSLS
Sbjct: 121 LYKLEAVEDVHILDLAWTLPCLQHNYLKKPEDYVAHLLGHEGNGSLHFSLKAKGWATSLS 180
Query: 184 ASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRELQD 243
A V +GM RSSMAY+F +SI LTDSGKEKI+EIIGYVYQ LKLLRQ+SPQEWIFRELQD
Sbjct: 181 AGVGDEGMCRSSMAYVFGMSIYLTDSGKEKIFEIIGYVYQYLKLLRQISPQEWIFRELQD 240
Query: 244 IGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTPENM 303
IGNM+FRFAEE+PQ+DYAA+LA NLS YPAEHVIYG Y YKIWDEDLV HIIGFFTPENM
Sbjct: 241 IGNMDFRFAEEQPQDDYAAELAENLSFYPAEHVIYGEYVYKIWDEDLVKHIIGFFTPENM 300
Query: 304 RIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFIPSD 363
R+DI SK FSKLEDFKIEPWFGSHYSVDDI SLMDLWRDPPEID SL+LP+KN+FIP D
Sbjct: 301 RVDIVSKSFSKLEDFKIEPWFGSHYSVDDIAPSLMDLWRDPPEIDASLHLPAKNQFIPCD 360
Query: 364 FSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSVKNN 423
FSIRA KVCN+LPLE SP CILDEPLMK WYKLDNSFK+PRA TYF INLSG YSSVKN
Sbjct: 361 FSIRASKVCNNLPLEYSPICILDEPLMKFWYKLDNSFKLPRANTYFHINLSGGYSSVKNY 420
Query: 424 LLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLATVT 483
LLTELF+ LLKDKLN II+QATIA L TS+A+ DKLELKVFGFNDKLPNLLS++LAT
Sbjct: 421 LLTELFVLLLKDKLNEIIYQATIAKLETSVAISGDKLELKVFGFNDKLPNLLSKLLATAR 480
Query: 484 TFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDVLND 543
TFMPSEDRF KVIKE ER L+N+ MKP H YLR +VL +RFYDADE+ DVLND
Sbjct: 481 TFMPSEDRF-----KVIKEKMERNLKNTNMKPRSHSSYLRLQVLCERFYDADEKSDVLND 540
Query: 544 LSFVDLKAHIPELLPS-FITDSCH 554
LSFVDLKAHIP+LL +I CH
Sbjct: 541 LSFVDLKAHIPKLLSQLYIEGLCH 559
BLAST of PI0011268 vs. TAIR 10
Match:
AT1G06900.1 (Insulinase (Peptidase family M16) family protein )
HSP 1 Score: 696.4 bits (1796), Expect = 1.8e-200
Identity = 349/566 (61.66%), Postives = 432/566 (76.33%), Query Frame = 0
Query: 2 ESMEREVLAVDSD-------QSCHVNRSFFISSFLAME------GNKKSLVDATEKGINL 61
E+MEREVLAVDS+ +C + + +S GNKKSL A E G++L
Sbjct: 198 EAMEREVLAVDSEFNQALQNDACRLQQLQCYTSAKGHPFNRFAWGNKKSLSGAMENGVDL 257
Query: 62 REQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKDPIWQS 121
RE I+KL+ +YYHGGLMKL VIGGESLD+LESWV+ELFG+VK G + +P + PIW+
Sbjct: 258 RECIVKLYKEYYHGGLMKLVVIGGESLDMLESWVVELFGDVKNGSKIRPTLEAEGPIWKG 317
Query: 122 GKLYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWATS 181
GKLY+LEAV+DVHIL L WTLP L++ Y+KKPE+Y+AHLL HEG+GSLH FLKAKGWATS
Sbjct: 318 GKLYRLEAVKDVHILDLTWTLPPLRSAYVKKPEDYLAHLLGHEGRGSLHSFLKAKGWATS 377
Query: 182 LSASVEKDGMHRSSMAYIFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFREL 241
LSA V DG++RSS+AY+F +SI+LTDSG EKIY+IIGY+YQ LKLLR VSPQEWIF+EL
Sbjct: 378 LSAGVGDDGINRSSLAYVFGMSIHLTDSGLEKIYDIIGYIYQYLKLLRDVSPQEWIFKEL 437
Query: 242 QDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTPE 301
QDIGNM+FRFAEE+P +DYAA+L+ N+ YP EHVIYG+Y Y+ WD L+ ++GFFTP+
Sbjct: 438 QDIGNMDFRFAEEQPADDYAAELSENMLAYPVEHVIYGDYVYQTWDPKLIEDLMGFFTPQ 497
Query: 302 NMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFIP 361
NMRID+ SK K E+F+ EPWFGS Y +D+P SLM+ W +P E+D SL+LPSKN+FIP
Sbjct: 498 NMRIDVVSKSI-KSEEFQQEPWFGSSYIEEDVPLSLMESWSNPSEVDNSLHLPSKNQFIP 557
Query: 362 SDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSVK 421
DFSIRA D +S PRCI+DEP MK WYKLD +FKVPRA TYFRINL GAY+SVK
Sbjct: 558 CDFSIRAINSDVDPKSQSPPRCIIDEPFMKFWYKLDETFKVPRANTYFRINLKGAYASVK 617
Query: 422 NNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLAT 481
N LLTEL+I LLKD+LN II+QA+IA L TS+++ DKLELKV+GFN+K+P LLS++LA
Sbjct: 618 NCLLTELYINLLKDELNEIIYQASIAKLETSLSMYGDKLELKVYGFNEKIPALLSKILAI 677
Query: 482 VTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYLRPKVLYKRFYDADERIDVL 541
+FMP+ +RF KVIKEN ER RN+ MKP H YLR ++L KR YD+DE++ VL
Sbjct: 678 AKSFMPNLERF-----KVIKENMERGFRNTNMKPLNHSTYLRLQLLCKRIYDSDEKLSVL 737
Query: 542 NDLSFVDLKAHIPELLPS-FITDSCH 554
NDLS DL + IPEL FI CH
Sbjct: 738 NDLSLDDLNSFIPELRSQIFIEALCH 757
BLAST of PI0011268 vs. TAIR 10
Match:
AT2G41790.1 (Insulinase (Peptidase family M16) family protein )
HSP 1 Score: 256.5 bits (654), Expect = 4.8e-68
Identity = 164/515 (31.84%), Postives = 260/515 (50.49%), Query Frame = 0
Query: 33 GNKKSL-VDATEKGINLREQILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKK 92
GN +L V KG++ R +++K + ++Y +M L V G ESLD ++ V +F E++
Sbjct: 182 GNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVERMFQEIQN 241
Query: 93 GVQAKPRFTVKDPIWQSGK-LYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRH 152
+ PRF + + L K ++ H L ++W + ++Y + P Y+ HL+ H
Sbjct: 242 TNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQYLGHLIGH 301
Query: 153 EGKGSLHFFLKAKGWATSLSASVEKDGMHRSSMAY-IFVLSINLTDSGKEKIYEIIGYVY 212
EG+GSL LK GWAT LSA G ++ Y F +SI+LTD+G E + EI+G ++
Sbjct: 302 EGEGSLFHALKTLGWATGLSA-----GEGEWTLDYSFFKVSIDLTDAGHEHMQEILGLLF 361
Query: 213 QCLKLLRQVSPQEWIFRELQDIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYA 272
++LL+Q +WIF EL I +F + ++ P Y +A N+ +YP + + G+
Sbjct: 362 NYIQLLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVGSSL 421
Query: 273 YKIWDEDLVNHIIGFFTPENMRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWR 332
++ +V ++ +P N RI +S+ F D K EPW+ + YS++ I +S + W
Sbjct: 422 PTKFNPAIVQKVVDELSPSNFRIFWESQKFEGQTD-KAEPWYNTAYSLEKITSSTIQEWV 481
Query: 333 DPPEIDTSLYLPSKNEFIPSDFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKV 392
D L+LP+ N FIP+D S++ E+ P + P +LWYK D F
Sbjct: 482 QSAP-DVHLHLPAPNVFIPTDLSLKDAD-----DKETVPVLLRKTPFSRLWYKPDTMFSK 541
Query: 393 PRACTYFRINLSGAYSSVKNNLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLEL 452
P+A N A SS +LT++F LL D LN + A +A L ++L D+ EL
Sbjct: 542 PKAYVKMDFNCPLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFEL 601
Query: 453 KVFGFNDKLPNLLSEVLATVTTFMPSEDRFKVIFIKVIKENFERVLRNSTMKPGIHLLYL 512
+ G+N KL LL V+ + F DRF VI V KE R +P +Y
Sbjct: 602 TLLGYNHKLRILLETVVGKIANFEVKPDRFAVIKETVTKEYQNYKFR----QPYHQAMYY 661
Query: 513 RPKVLYKRFYDADERIDVLNDLSFVDLKAHIPELL 545
+L + + E +DVL+ L D+ +P LL
Sbjct: 662 CSLILQDQTWPWTEELDVLSHLEAEDVAKFVPMLL 680
BLAST of PI0011268 vs. TAIR 10
Match:
AT3G57470.1 (Insulinase (Peptidase family M16) family protein )
HSP 1 Score: 244.6 bits (623), Expect = 1.9e-64
Identity = 171/556 (30.76%), Postives = 271/556 (48.74%), Query Frame = 0
Query: 6 REVLAVDSDQSCHVN----RSFFISSFLAME---------GNKKSL-VDATEKGINLREQ 65
RE+ AVDS+ ++ R + L+ E GN +L V E G++ R +
Sbjct: 144 REIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEENGVDTRSE 203
Query: 66 ILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKD-PIWQSGK 125
++K + ++Y +M L V G E+LD + V LF ++ Q PRF + +
Sbjct: 204 LIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQPCTLDHLQV 263
Query: 126 LYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWATSLS 185
L K + H L+++W + ++Y + P Y+ L+ HEG+GSL LK GWAT L
Sbjct: 264 LVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKILGWATGLY 323
Query: 186 ASVEKDGMHRSSMAY-IFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRELQ 245
A G SM Y F +SI+LTD+G E + +I+G +++ +K+L+Q +WIF EL
Sbjct: 324 A-----GEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFDELS 383
Query: 246 DIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTPEN 305
I EF + + YA ++ N+ +YP +H + G+ ++ +V ++ +P N
Sbjct: 384 AICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELSPNN 443
Query: 306 MRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFIPS 365
+RI +S F D K+EPW+ + YS++ I + W D +L LP+ N FIP+
Sbjct: 444 VRIFWESNKFEGQTD-KVEPWYNTAYSLEKITKFTIQEWMQSAP-DVNLLLPTPNVFIPT 503
Query: 366 DFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSVKN 425
DFS++ K + P+ + +LWYK D F P+A N A SS
Sbjct: 504 DFSLKDLKDKDIFPV-----LLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDA 563
Query: 426 NLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLATV 485
+L+++F+ LL D LN + A A L ++L D+ EL + GFN KL LL V+ +
Sbjct: 564 AVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKLRILLEAVIQKI 623
Query: 486 TTFMPSEDRFKVIFIKVIKENFERVLRNST-MKPGIHLLYLRPKVLYKRFYDADERIDVL 545
F DRF VIKE + +N+ +P VL + + E +D L
Sbjct: 624 AKFEVKPDRF-----SVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWTEELDAL 682
BLAST of PI0011268 vs. TAIR 10
Match:
AT3G57470.2 (Insulinase (Peptidase family M16) family protein )
HSP 1 Score: 244.6 bits (623), Expect = 1.9e-64
Identity = 171/556 (30.76%), Postives = 271/556 (48.74%), Query Frame = 0
Query: 6 REVLAVDSDQSCHVN----RSFFISSFLAME---------GNKKSL-VDATEKGINLREQ 65
RE+ AVDS+ ++ R + L+ E GN +L V E G++ R +
Sbjct: 69 REIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEENGVDTRSE 128
Query: 66 ILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKD-PIWQSGK 125
++K + ++Y +M L V G E+LD + V LF ++ Q PRF + +
Sbjct: 129 LIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQPCTLDHLQV 188
Query: 126 LYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWATSLS 185
L K + H L+++W + ++Y + P Y+ L+ HEG+GSL LK GWAT L
Sbjct: 189 LVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKILGWATGLY 248
Query: 186 ASVEKDGMHRSSMAY-IFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRELQ 245
A G SM Y F +SI+LTD+G E + +I+G +++ +K+L+Q +WIF EL
Sbjct: 249 A-----GEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFDELS 308
Query: 246 DIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTPEN 305
I EF + + YA ++ N+ +YP +H + G+ ++ +V ++ +P N
Sbjct: 309 AICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELSPNN 368
Query: 306 MRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFIPS 365
+RI +S F D K+EPW+ + YS++ I + W D +L LP+ N FIP+
Sbjct: 369 VRIFWESNKFEGQTD-KVEPWYNTAYSLEKITKFTIQEWMQSAP-DVNLLLPTPNVFIPT 428
Query: 366 DFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSVKN 425
DFS++ K + P+ + +LWYK D F P+A N A SS
Sbjct: 429 DFSLKDLKDKDIFPV-----LLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDA 488
Query: 426 NLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLATV 485
+L+++F+ LL D LN + A A L ++L D+ EL + GFN KL LL V+ +
Sbjct: 489 AVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKLRILLEAVIQKI 548
Query: 486 TTFMPSEDRFKVIFIKVIKENFERVLRNST-MKPGIHLLYLRPKVLYKRFYDADERIDVL 545
F DRF VIKE + +N+ +P VL + + E +D L
Sbjct: 549 AKFEVKPDRF-----SVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWTEELDAL 607
BLAST of PI0011268 vs. TAIR 10
Match:
AT3G57470.3 (Insulinase (Peptidase family M16) family protein )
HSP 1 Score: 244.6 bits (623), Expect = 1.9e-64
Identity = 171/556 (30.76%), Postives = 271/556 (48.74%), Query Frame = 0
Query: 6 REVLAVDSDQSCHVN----RSFFISSFLAME---------GNKKSL-VDATEKGINLREQ 65
RE+ AVDS+ ++ R + L+ E GN +L V E G++ R +
Sbjct: 69 REIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEENGVDTRSE 128
Query: 66 ILKLFGDYYHGGLMKLTVIGGESLDVLESWVLELFGEVKKGVQAKPRFTVKD-PIWQSGK 125
++K + ++Y +M L V G E+LD + V LF ++ Q PRF + +
Sbjct: 129 LIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQPCTLDHLQV 188
Query: 126 LYKLEAVQDVHILALAWTLPCLQNNYLKKPENYIAHLLRHEGKGSLHFFLKAKGWATSLS 185
L K + H L+++W + ++Y + P Y+ L+ HEG+GSL LK GWAT L
Sbjct: 189 LVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKILGWATGLY 248
Query: 186 ASVEKDGMHRSSMAY-IFVLSINLTDSGKEKIYEIIGYVYQCLKLLRQVSPQEWIFRELQ 245
A G SM Y F +SI+LTD+G E + +I+G +++ +K+L+Q +WIF EL
Sbjct: 249 A-----GEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFDELS 308
Query: 246 DIGNMEFRFAEEEPQNDYAAKLAVNLSVYPAEHVIYGNYAYKIWDEDLVNHIIGFFTPEN 305
I EF + + YA ++ N+ +YP +H + G+ ++ +V ++ +P N
Sbjct: 309 AICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELSPNN 368
Query: 306 MRIDIQSKDFSKLEDFKIEPWFGSHYSVDDIPTSLMDLWRDPPEIDTSLYLPSKNEFIPS 365
+RI +S F D K+EPW+ + YS++ I + W D +L LP+ N FIP+
Sbjct: 369 VRIFWESNKFEGQTD-KVEPWYNTAYSLEKITKFTIQEWMQSAP-DVNLLLPTPNVFIPT 428
Query: 366 DFSIRACKVCNDLPLESSPRCILDEPLMKLWYKLDNSFKVPRACTYFRINLSGAYSSVKN 425
DFS++ K + P+ + +LWYK D F P+A N A SS
Sbjct: 429 DFSLKDLKDKDIFPV-----LLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDA 488
Query: 426 NLLTELFIELLKDKLNVIIFQATIANLGTSMALDDDKLELKVFGFNDKLPNLLSEVLATV 485
+L+++F+ LL D LN + A A L ++L D+ EL + GFN KL LL V+ +
Sbjct: 489 AVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKLRILLEAVIQKI 548
Query: 486 TTFMPSEDRFKVIFIKVIKENFERVLRNST-MKPGIHLLYLRPKVLYKRFYDADERIDVL 545
F DRF VIKE + +N+ +P VL + + E +D L
Sbjct: 549 AKFEVKPDRF-----SVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWTEELDAL 607
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4HNU6 | 2.6e-199 | 61.66 | Nardilysin-like OS=Arabidopsis thaliana OX=3702 GN=At1g06900 PE=2 SV=1 | [more] |
| P14735 | 1.1e-77 | 33.57 | Insulin-degrading enzyme OS=Homo sapiens OX=9606 GN=IDE PE=1 SV=4 | [more] |
| Q24K02 | 9.4e-77 | 33.39 | Insulin-degrading enzyme OS=Bos taurus OX=9913 GN=IDE PE=2 SV=1 | [more] |
| P35559 | 1.2e-76 | 33.09 | Insulin-degrading enzyme OS=Rattus norvegicus OX=10116 GN=Ide PE=1 SV=1 | [more] |
| Q9JHR7 | 5.2e-75 | 33.04 | Insulin-degrading enzyme OS=Mus musculus OX=10090 GN=Ide PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5D3DT90 | 1.5e-250 | 78.31 | Nardilysin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold260G0022... | [more] |
| A0A5A7SU38 | 1.5e-250 | 78.31 | Nardilysin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold261G0033... | [more] |
| A0A1S3CNH0 | 1.5e-250 | 78.31 | nardilysin-like OS=Cucumis melo OX=3656 GN=LOC103502959 PE=3 SV=1 | [more] |
| A0A6J1FUR8 | 7.4e-242 | 75.31 | nardilysin-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448618 PE=3 SV... | [more] |
| A0A6J1FXN2 | 7.4e-242 | 75.31 | nardilysin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448618 PE=3 SV... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_038901221.1 | 4.7e-251 | 78.27 | nardilysin-like [Benincasa hispida] | [more] |
| XP_008465304.1 | 3.1e-250 | 78.31 | PREDICTED: nardilysin-like [Cucumis melo] >KAA0033496.1 nardilysin-like [Cucumis... | [more] |
| TYK26833.1 | 3.1e-250 | 78.31 | nardilysin-like [Cucumis melo var. makuwa] | [more] |
| XP_004150605.1 | 2.2e-248 | 77.56 | nardilysin-like isoform X1 [Cucumis sativus] >KAE8647021.1 hypothetical protein ... | [more] |
| XP_031743090.1 | 8.4e-248 | 77.66 | nardilysin-like isoform X2 [Cucumis sativus] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT1G06900.1 | 1.8e-200 | 61.66 | Insulinase (Peptidase family M16) family protein | [more] |
| AT2G41790.1 | 4.8e-68 | 31.84 | Insulinase (Peptidase family M16) family protein | [more] |
| AT3G57470.1 | 1.9e-64 | 30.76 | Insulinase (Peptidase family M16) family protein | [more] |
| AT3G57470.2 | 1.9e-64 | 30.76 | Insulinase (Peptidase family M16) family protein | [more] |
| AT3G57470.3 | 1.9e-64 | 30.76 | Insulinase (Peptidase family M16) family protein | [more] |
