PI0010812 (gene) Melon (PI 482460) v1

Overview
NamePI0010812
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr11: 26560526 .. 26561482 (-)
RNA-Seq ExpressionPI0010812
SyntenyPI0010812
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCGATGAAATTGCCTTAAACGTTGCACCTTATTTCATCGTTTACAAAGACGGTCGAATCGACAGGTTCATCGGCAACGATATCGATCCTCCCGGTCTCGACCCCAAAACCGGCGTCGAGTCAAAAGACGTCGACATCTCGCCGGACGTCGCCGTTCGTGTTTACAGACCCAAATCCGCCGCCGAAAAACAATCTGAGAAGCTCCCTCTTCTCGTTTACTTTCACGGCGGTGGGTTCTGTATCGAAACCGCATTTTCCCCTTTCTACCATCAGCACATCAGCGCTTGGGTAGCCGAAGCCAACATCACCGCCGTCTCCGTCAATTACAGGCGAGCACCGGAGCACCAGTTACCGATTCCTTTCGAAGATGCATGGACCGCCATGAAATGGATCGCGTCCCACTCGGGAGGAAAAGGACCGGATGAGTGGCTGAACGAAATCGCAGATCTGAACCAGGTTTATTTAGCAGGAGACAGCGCCGGAGGAAACATGGCACACAGGATGGCGTTGAGGACGGTGACGGAAGGGCTTGAGGGAGTGAAGATCAAGGGATTGCAATTGATTCACCCACATTTCTGGGGGGGGAAATTGATCGGAGAAGAAAATGATTGGGATCCAAAGGACTTATTCGTAGTGGAGAATCTGTGGTTTGTAGTTTCGAAGGATATAAAGACACTAGATGATCCGATTGTGAACCCAGAGCACGATCCGGATTTGGGGCGGCTACCGGTGGAGAGAGTGGGGGTGTATGTGGCGGAGAAGGACAATCTGAAGGAGAGGGGAAGGCATTATGCAGAGTGTTTGAAGAAATCGGGATGGGGTGGAACAGTGGAGGTGGTGGAGACGGAAGGCGAAGGACACGTATTCCATTTGTTCAATCCGACGTGTGATATGGCGGGGGAATTGGTGAAACAGCTGGCGGCTTTCATCAAGAGCGGCTGCCATGGGTAA

mRNA sequence

ATGGCTTCCGATGAAATTGCCTTAAACGTTGCACCTTATTTCATCGTTTACAAAGACGGTCGAATCGACAGGTTCATCGGCAACGATATCGATCCTCCCGGTCTCGACCCCAAAACCGGCGTCGAGTCAAAAGACGTCGACATCTCGCCGGACGTCGCCGTTCGTGTTTACAGACCCAAATCCGCCGCCGAAAAACAATCTGAGAAGCTCCCTCTTCTCGTTTACTTTCACGGCGGTGGGTTCTGTATCGAAACCGCATTTTCCCCTTTCTACCATCAGCACATCAGCGCTTGGGTAGCCGAAGCCAACATCACCGCCGTCTCCGTCAATTACAGGCGAGCACCGGAGCACCAGTTACCGATTCCTTTCGAAGATGCATGGACCGCCATGAAATGGATCGCGTCCCACTCGGGAGGAAAAGGACCGGATGAGTGGCTGAACGAAATCGCAGATCTGAACCAGGTTTATTTAGCAGGAGACAGCGCCGGAGGAAACATGGCACACAGGATGGCGTTGAGGACGGTGACGGAAGGGCTTGAGGGAGTGAAGATCAAGGGATTGCAATTGATTCACCCACATTTCTGGGGGGGGAAATTGATCGGAGAAGAAAATGATTGGGATCCAAAGGACTTATTCGTAGTGGAGAATCTGTGGTTTGTAGTTTCGAAGGATATAAAGACACTAGATGATCCGATTGTGAACCCAGAGCACGATCCGGATTTGGGGCGGCTACCGGTGGAGAGAGTGGGGGTGTATGTGGCGGAGAAGGACAATCTGAAGGAGAGGGGAAGGCATTATGCAGAGTGTTTGAAGAAATCGGGATGGGGTGGAACAGTGGAGGTGGTGGAGACGGAAGGCGAAGGACACGTATTCCATTTGTTCAATCCGACGTGTGATATGGCGGGGGAATTGGTGAAACAGCTGGCGGCTTTCATCAAGAGCGGCTGCCATGGGTAA

Coding sequence (CDS)

ATGGCTTCCGATGAAATTGCCTTAAACGTTGCACCTTATTTCATCGTTTACAAAGACGGTCGAATCGACAGGTTCATCGGCAACGATATCGATCCTCCCGGTCTCGACCCCAAAACCGGCGTCGAGTCAAAAGACGTCGACATCTCGCCGGACGTCGCCGTTCGTGTTTACAGACCCAAATCCGCCGCCGAAAAACAATCTGAGAAGCTCCCTCTTCTCGTTTACTTTCACGGCGGTGGGTTCTGTATCGAAACCGCATTTTCCCCTTTCTACCATCAGCACATCAGCGCTTGGGTAGCCGAAGCCAACATCACCGCCGTCTCCGTCAATTACAGGCGAGCACCGGAGCACCAGTTACCGATTCCTTTCGAAGATGCATGGACCGCCATGAAATGGATCGCGTCCCACTCGGGAGGAAAAGGACCGGATGAGTGGCTGAACGAAATCGCAGATCTGAACCAGGTTTATTTAGCAGGAGACAGCGCCGGAGGAAACATGGCACACAGGATGGCGTTGAGGACGGTGACGGAAGGGCTTGAGGGAGTGAAGATCAAGGGATTGCAATTGATTCACCCACATTTCTGGGGGGGGAAATTGATCGGAGAAGAAAATGATTGGGATCCAAAGGACTTATTCGTAGTGGAGAATCTGTGGTTTGTAGTTTCGAAGGATATAAAGACACTAGATGATCCGATTGTGAACCCAGAGCACGATCCGGATTTGGGGCGGCTACCGGTGGAGAGAGTGGGGGTGTATGTGGCGGAGAAGGACAATCTGAAGGAGAGGGGAAGGCATTATGCAGAGTGTTTGAAGAAATCGGGATGGGGTGGAACAGTGGAGGTGGTGGAGACGGAAGGCGAAGGACACGTATTCCATTTGTTCAATCCGACGTGTGATATGGCGGGGGAATTGGTGAAACAGCTGGCGGCTTTCATCAAGAGCGGCTGCCATGGGTAA

Protein sequence

MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPDVAVRVYRPKSAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLPIPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEGVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPDLGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDMAGELVKQLAAFIKSGCHG
Homology
BLAST of PI0010812 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 3.3e-82
Identity = 154/320 (48.12%), Postives = 206/320 (64.38%), Query Frame = 0

Query: 5   EIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYRPKSA 64
           EIA++ +P   +YK GRI+R +G    PP  +P+ GV SKDV  S D  ++VR+Y P+ A
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 65  AEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLPIP 124
           A +   KLPLLVYFHGGGF IETAFSP YH  ++  V+ +N  AVSV+YRRAPEH + +P
Sbjct: 64  AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 123

Query: 125 FEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTE----G 184
           F+D+WTA+KW+ +H  G G ++WLN+ AD ++V+L+GDSAG N+ H MA+R   E    G
Sbjct: 124 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPG 183

Query: 185 LEGVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKT-LDDPIVN--P 244
           L    I G+ L+HP+FW    I E++  D      +E  W + S + K   DDP++N   
Sbjct: 184 LNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQ 243

Query: 245 EHDPDLGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFN 304
               DL  L   +V V VAEKD L  +G  YA  L+KSGW G VEVVE+EGE HVFHL  
Sbjct: 244 SESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLK 303

Query: 305 PTCDMAGELVKQLAAFIKSG 316
           P CD A E++ + + FIK G
Sbjct: 304 PECDNAIEVMHKFSGFIKGG 323

BLAST of PI0010812 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 5.4e-77
Identity = 150/323 (46.44%), Postives = 205/323 (63.47%), Query Frame = 0

Query: 5   EIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYRPKSA 64
           EIA + +P  I+YK GRI+R +G    PP  +P+ GV SKDV  SPD  +++R+Y P+ A
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 65  AEKQSE---KLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQL 124
           A  ++E   KLPLLVYFHGGGF +ETAFSP YH  ++A V+ ++  AVSV+YRRAPEH +
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 125 PIPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTV---- 184
           P  ++D+WTA+KW+ SH  G G ++WLN+ AD ++V+LAGDSAG N+ H M ++      
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 185 -TEGLEGVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKT-LDDPIV 244
             E L    I G+ L+HP+FW    + ++   D      +E++W + S + K   DDP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 245 N--PEHDPDLGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTV-EVVETEGEGHV 304
           N       DL  L   +V V VAEKD L  +G  Y E L KS W G V +VVET+GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

Query: 305 FHLFNPTCDMAGELVKQLAAFIK 314
           FHL +P  + A ELV + A FIK
Sbjct: 304 FHLRDPNSEKAHELVHRFAGFIK 326

BLAST of PI0010812 vs. ExPASy Swiss-Prot
Match: Q9ZQ91 (Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.0e-75
Identity = 151/310 (48.71%), Postives = 196/310 (63.23%), Query Frame = 0

Query: 6   IALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYRPKSAA 65
           IA + +P F VYK GRI+R +G    PP L P+ GV SKD+  SP+  +++R+Y P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  EKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLPIPF 125
            K   KLP+L+YFHGGGF IETAFSP YH  +++ VA AN  A+SVNYRRAPE  +PIP+
Sbjct: 65  VK---KLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPY 124

Query: 126 EDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEGVK 185
           ED+W ++KW+ +H  G GP+ W+N+  D  +V+LAGDSAGGN++H + +R   E L    
Sbjct: 125 EDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSL 184

Query: 186 IKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKT-LDDPIVNPEHDPDLG 245
           I G+ LIHP+FW    I E    D      VE  W V S + K  +DDP +N       G
Sbjct: 185 ISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDPSG 244

Query: 246 RLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDMAG 305
            L   RV V VA  D    +G  YAE LKKSGW G VEV+ET+ EGHVFHL NP  D A 
Sbjct: 245 -LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNAR 304

Query: 306 ELVKQLAAFI 313
           ++VK+L  FI
Sbjct: 305 QVVKKLEEFI 310

BLAST of PI0010812 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.3e-74
Identity = 144/312 (46.15%), Postives = 202/312 (64.74%), Query Frame = 0

Query: 5   EIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISP--DVAVRVYRPKSA 64
           +++L + P+ +V+ DG ++R  G ++ PPGLDP TGV SKD+ I P   ++ R+YRP S 
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLPIP 124
             +  +K+PL++YFHGG F I +   P YH  ++  V +AN+ AVSVNYR APEH LP  
Sbjct: 67  --QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTA 126

Query: 125 FEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEGV 184
           +ED+WTA+K I + +     + W+N+ ADL+ ++L GDSAG N++H +A R   +  + +
Sbjct: 127 YEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQTL 186

Query: 185 KIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHD--PD 244
           KIKG+ +IHP+FWG + IG E   D     +V+  W  V    K  DDP +NP  D  PD
Sbjct: 187 KIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPD 246

Query: 245 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 304
           LG L  ERV + VAEKD L ERG+ Y E L KS W G VE++ET+ + HVFH+F P CD 
Sbjct: 247 LGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDE 306

Query: 305 AGELVKQLAAFI 313
           A E+V+ LA FI
Sbjct: 307 AMEMVRCLALFI 309

BLAST of PI0010812 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.4e-71
Identity = 139/314 (44.27%), Postives = 189/314 (60.19%), Query Frame = 0

Query: 5   EIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYRP-KS 64
           EIA    P+  +YKDGR++R IG D  P  LDP   V SKDV  SP+  ++VR++ P KS
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKS 63

Query: 65  AAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLPI 124
                  KLPLL+Y HGG + IE+ FSP YH +++  V  AN  AVSV YRRAPE  +P 
Sbjct: 64  TKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVPA 123

Query: 125 PFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEG 184
            +ED W+A++WI +HS G GP +W+N+ AD  +V+L GDSAGGN++H MA++   E    
Sbjct: 124 AYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKLD 183

Query: 185 VKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVS--KDIKTLDDPIVNPE-HD 244
           +KIKG+ ++HP FWG   + E +  D +    +  +W  ++    +   DDP+ N     
Sbjct: 184 LKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGSG 243

Query: 245 PDLGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTC 304
            D   L  ++V V VA KD    +G  YA  L+K  W GTVEVVE EGE HVFHL NP  
Sbjct: 244 SDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPKS 303

Query: 305 DMAGELVKQLAAFI 313
           D A + +K+   FI
Sbjct: 304 DKALKFLKKFVEFI 317

BLAST of PI0010812 vs. ExPASy TrEMBL
Match: A0A0A0LKY5 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G234510 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 7.6e-183
Identity = 303/318 (95.28%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPDVAVRVYRPK 60
           MASD+IALNVAP+FI+YKDGRIDR IGNDIDPPGLDPKTGVE+KDVDISPDVAVRVYRPK
Sbjct: 1   MASDDIALNVAPFFILYKDGRIDRLIGNDIDPPGLDPKTGVETKDVDISPDVAVRVYRPK 60

Query: 61  SAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLP 120
           S  EKQSEKLPLLVYFHGGGFCIETAFSPFY+QHISAWVAEANI AVSVNYRRAPEHQLP
Sbjct: 61  SPDEKQSEKLPLLVYFHGGGFCIETAFSPFYNQHISAWVAEANIAAVSVNYRRAPEHQLP 120

Query: 121 IPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180
           IPFEDAWTAMKWIASHS GKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE
Sbjct: 121 IPFEDAWTAMKWIASHSEGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180

Query: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240
           GVKIKGLQLIHPHFWGG+L+GEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD
Sbjct: 181 GVKIKGLQLIHPHFWGGELLGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240

Query: 241 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300
           LGRLP ERVG+YVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM
Sbjct: 241 LGRLPAERVGIYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300

Query: 301 AGELVKQLAAFIKSGCHG 319
           AGELVKQLAAFIKSGC G
Sbjct: 301 AGELVKQLAAFIKSGCRG 318

BLAST of PI0010812 vs. ExPASy TrEMBL
Match: A0A5A7SP21 (Putative carboxylesterase 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold223G00540 PE=4 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 4.2e-181
Identity = 303/318 (95.28%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPDVAVRVYRPK 60
           MASD+IALN APYFIVYKDGRIDR IGNDIDPPGLDP TGVESKDVDISPDV VRVYRPK
Sbjct: 1   MASDDIALNAAPYFIVYKDGRIDRLIGNDIDPPGLDPITGVESKDVDISPDVTVRVYRPK 60

Query: 61  SAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLP 120
           SAAEK SEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANI AVSVNYRRAPEHQLP
Sbjct: 61  SAAEKISEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANIIAVSVNYRRAPEHQLP 120

Query: 121 IPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180
           IPFEDAWT+MKWIASHS GKG DEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE
Sbjct: 121 IPFEDAWTSMKWIASHSEGKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180

Query: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240
           GVKIKGLQLIHPHFWGGKLIGEENDWDP DLFVVENLWFVVSKDIKTLDDPIVNPEHDPD
Sbjct: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240

Query: 241 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300
           LGRLP ERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCD+
Sbjct: 241 LGRLPAERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDI 300

Query: 301 AGELVKQLAAFIKSGCHG 319
           AGELVKQLAAFI SGC G
Sbjct: 301 AGELVKQLAAFINSGCRG 318

BLAST of PI0010812 vs. ExPASy TrEMBL
Match: A0A1S3CA69 (probable carboxylesterase 12 OS=Cucumis melo OX=3656 GN=LOC103498399 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 7.1e-181
Identity = 302/318 (94.97%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPDVAVRVYRPK 60
           MASD+IALN APYFIVYKDGRIDR IGNDIDPPGLDP TGVESKDVDISPDV VRVYRPK
Sbjct: 1   MASDDIALNAAPYFIVYKDGRIDRLIGNDIDPPGLDPITGVESKDVDISPDVTVRVYRPK 60

Query: 61  SAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLP 120
           SAAEK SEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANI AVSVNYRRAPEHQLP
Sbjct: 61  SAAEKISEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANIIAVSVNYRRAPEHQLP 120

Query: 121 IPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180
           IPFEDAWT+MKWIASHS GKG DEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE
Sbjct: 121 IPFEDAWTSMKWIASHSEGKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180

Query: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240
           GVKIKGLQLIHPHFWGGKLIGEENDWDP DLFVVENLWFVVSKDIKTLDDPIVNPEHDPD
Sbjct: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240

Query: 241 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300
           LGR+P ERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCD+
Sbjct: 241 LGRMPAERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDI 300

Query: 301 AGELVKQLAAFIKSGCHG 319
           AGELVKQLAAFI SGC G
Sbjct: 301 AGELVKQLAAFINSGCRG 318

BLAST of PI0010812 vs. ExPASy TrEMBL
Match: A0A5D3CQ21 (Putative carboxylesterase 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00140 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 7.1e-181
Identity = 302/318 (94.97%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPDVAVRVYRPK 60
           MASD+IALN APYFIVYKDGRIDR IGNDIDPPGLDP TGVESKDVDISPDV VRVYRPK
Sbjct: 1   MASDDIALNAAPYFIVYKDGRIDRLIGNDIDPPGLDPITGVESKDVDISPDVTVRVYRPK 60

Query: 61  SAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLP 120
           SAAEK SEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANI AVSVNYRRAPEHQLP
Sbjct: 61  SAAEKISEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANIIAVSVNYRRAPEHQLP 120

Query: 121 IPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180
           IPFEDAWT+MKWIASHS GKG DEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE
Sbjct: 121 IPFEDAWTSMKWIASHSEGKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180

Query: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240
           GVKIKGLQLIHPHFWGGKLIGEENDWDP DLFVVENLWFVVSKDIKTLDDPIVNPEHDPD
Sbjct: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240

Query: 241 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300
           LGR+P ERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCD+
Sbjct: 241 LGRMPAERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDI 300

Query: 301 AGELVKQLAAFIKSGCHG 319
           AGELVKQLAAFI SGC G
Sbjct: 301 AGELVKQLAAFINSGCRG 318

BLAST of PI0010812 vs. ExPASy TrEMBL
Match: A0A6J1FSN2 (probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448111 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 3.9e-163
Identity = 269/318 (84.59%), Postives = 293/318 (92.14%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYR 60
           MASDEIALN  PYF+VYKDGRIDR IGND DPPG+DPKTGVESKDV +SP+  V+VR+YR
Sbjct: 1   MASDEIALNAVPYFVVYKDGRIDRLIGNDFDPPGVDPKTGVESKDVVVSPEDSVSVRIYR 60

Query: 61  PKSAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQ 120
           PKSAAE Q++KLPLLVYFHGGGFCIETAFSPFYHQHI+AWVA+ANI AVSVNYRRAPE+Q
Sbjct: 61  PKSAAE-QAQKLPLLVYFHGGGFCIETAFSPFYHQHINAWVAKANIAAVSVNYRRAPEYQ 120

Query: 121 LPIPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEG 180
           LPIPFED+WTAMKW+A+HSGG GPDEWLNEIADLN+VYLAGDSAGGN+AHRMALR  TEG
Sbjct: 121 LPIPFEDSWTAMKWVAAHSGGAGPDEWLNEIADLNRVYLAGDSAGGNIAHRMALRVATEG 180

Query: 181 LEGVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHD 240
           L GVKIKGLQLIHPHFWGGKLIGEE +WDPKD+FVVENLW+VVSKDIK LDDPIVNPEHD
Sbjct: 181 LAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKDIFVVENLWYVVSKDIKELDDPIVNPEHD 240

Query: 241 PDLGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTC 300
           PDL RLP E+V VYVAEKDNLKERGRHYAECL+KSGWGG VEVVET+GEGHVFHLFNPTC
Sbjct: 241 PDLARLPAEKVAVYVAEKDNLKERGRHYAECLRKSGWGGAVEVVETDGEGHVFHLFNPTC 300

Query: 301 DMAGELVKQLAAFIKSGC 317
           D A +LV+ LAAF  S C
Sbjct: 301 DSAADLVELLAAFTSSDC 317

BLAST of PI0010812 vs. NCBI nr
Match: XP_004145409.1 (probable carboxylesterase 12 [Cucumis sativus] >KGN61724.1 hypothetical protein Csa_005943 [Cucumis sativus])

HSP 1 Score: 649.4 bits (1674), Expect = 1.6e-182
Identity = 303/318 (95.28%), Postives = 311/318 (97.80%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPDVAVRVYRPK 60
           MASD+IALNVAP+FI+YKDGRIDR IGNDIDPPGLDPKTGVE+KDVDISPDVAVRVYRPK
Sbjct: 1   MASDDIALNVAPFFILYKDGRIDRLIGNDIDPPGLDPKTGVETKDVDISPDVAVRVYRPK 60

Query: 61  SAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLP 120
           S  EKQSEKLPLLVYFHGGGFCIETAFSPFY+QHISAWVAEANI AVSVNYRRAPEHQLP
Sbjct: 61  SPDEKQSEKLPLLVYFHGGGFCIETAFSPFYNQHISAWVAEANIAAVSVNYRRAPEHQLP 120

Query: 121 IPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180
           IPFEDAWTAMKWIASHS GKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE
Sbjct: 121 IPFEDAWTAMKWIASHSEGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180

Query: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240
           GVKIKGLQLIHPHFWGG+L+GEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD
Sbjct: 181 GVKIKGLQLIHPHFWGGELLGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240

Query: 241 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300
           LGRLP ERVG+YVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM
Sbjct: 241 LGRLPAERVGIYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300

Query: 301 AGELVKQLAAFIKSGCHG 319
           AGELVKQLAAFIKSGC G
Sbjct: 301 AGELVKQLAAFIKSGCRG 318

BLAST of PI0010812 vs. NCBI nr
Match: KAA0032083.1 (putative carboxylesterase 12 [Cucumis melo var. makuwa])

HSP 1 Score: 643.7 bits (1659), Expect = 8.6e-181
Identity = 303/318 (95.28%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPDVAVRVYRPK 60
           MASD+IALN APYFIVYKDGRIDR IGNDIDPPGLDP TGVESKDVDISPDV VRVYRPK
Sbjct: 1   MASDDIALNAAPYFIVYKDGRIDRLIGNDIDPPGLDPITGVESKDVDISPDVTVRVYRPK 60

Query: 61  SAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLP 120
           SAAEK SEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANI AVSVNYRRAPEHQLP
Sbjct: 61  SAAEKISEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANIIAVSVNYRRAPEHQLP 120

Query: 121 IPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180
           IPFEDAWT+MKWIASHS GKG DEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE
Sbjct: 121 IPFEDAWTSMKWIASHSEGKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180

Query: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240
           GVKIKGLQLIHPHFWGGKLIGEENDWDP DLFVVENLWFVVSKDIKTLDDPIVNPEHDPD
Sbjct: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240

Query: 241 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300
           LGRLP ERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCD+
Sbjct: 241 LGRLPAERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDI 300

Query: 301 AGELVKQLAAFIKSGCHG 319
           AGELVKQLAAFI SGC G
Sbjct: 301 AGELVKQLAAFINSGCRG 318

BLAST of PI0010812 vs. NCBI nr
Match: XP_008459207.1 (PREDICTED: probable carboxylesterase 12 [Cucumis melo] >XP_008459208.1 PREDICTED: probable carboxylesterase 12 [Cucumis melo] >TYK13540.1 putative carboxylesterase 12 [Cucumis melo var. makuwa])

HSP 1 Score: 642.9 bits (1657), Expect = 1.5e-180
Identity = 302/318 (94.97%), Postives = 306/318 (96.23%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPDVAVRVYRPK 60
           MASD+IALN APYFIVYKDGRIDR IGNDIDPPGLDP TGVESKDVDISPDV VRVYRPK
Sbjct: 1   MASDDIALNAAPYFIVYKDGRIDRLIGNDIDPPGLDPITGVESKDVDISPDVTVRVYRPK 60

Query: 61  SAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLP 120
           SAAEK SEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANI AVSVNYRRAPEHQLP
Sbjct: 61  SAAEKISEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANIIAVSVNYRRAPEHQLP 120

Query: 121 IPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180
           IPFEDAWT+MKWIASHS GKG DEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE
Sbjct: 121 IPFEDAWTSMKWIASHSEGKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180

Query: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240
           GVKIKGLQLIHPHFWGGKLIGEENDWDP DLFVVENLWFVVSKDIKTLDDPIVNPEHDPD
Sbjct: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240

Query: 241 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300
           LGR+P ERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCD+
Sbjct: 241 LGRMPAERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDI 300

Query: 301 AGELVKQLAAFIKSGCHG 319
           AGELVKQLAAFI SGC G
Sbjct: 301 AGELVKQLAAFINSGCRG 318

BLAST of PI0010812 vs. NCBI nr
Match: XP_038900341.1 (probable carboxylesterase 7 [Benincasa hispida])

HSP 1 Score: 610.5 bits (1573), Expect = 8.1e-171
Identity = 286/318 (89.94%), Postives = 298/318 (93.71%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPDVAVRVYRPK 60
           MASDEI+LN APYFIVYKD RIDR IGNDIDPPGLDP TGVESKDVDISP+VAVRVYRPK
Sbjct: 1   MASDEISLNAAPYFIVYKDSRIDRLIGNDIDPPGLDPNTGVESKDVDISPEVAVRVYRPK 60

Query: 61  SAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLP 120
           SAA KQ EKLPLLVYFHGGGFCIETAFSPFYHQHI+AWVAE NI AVSVNYRRAPE+QLP
Sbjct: 61  SAAGKQ-EKLPLLVYFHGGGFCIETAFSPFYHQHINAWVAEGNIAAVSVNYRRAPEYQLP 120

Query: 121 IPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLE 180
           IPFED+W AMKWIA+HS GKGPDEWLNEIADLNQVYLAGDSAGGN+AHRMALR  TEGL+
Sbjct: 121 IPFEDSWAAMKWIAAHSEGKGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRVATEGLD 180

Query: 181 GVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHDPD 240
           GVKIKGLQLIHPHFWGGKLIGEE +WDPKDLFVVENLWFVVSKDIKTLD PIVNPEHDPD
Sbjct: 181 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKDLFVVENLWFVVSKDIKTLDHPIVNPEHDPD 240

Query: 241 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 300
           LG+LP ERV VYVAEKDNLK RGRHYAECLKKSGWGGTVEVVET+GEGHVFHLFNPTCDM
Sbjct: 241 LGKLPAERVAVYVAEKDNLKVRGRHYAECLKKSGWGGTVEVVETDGEGHVFHLFNPTCDM 300

Query: 301 AGELVKQLAAFIKSGCHG 319
           AGEL+K LAAFI SG HG
Sbjct: 301 AGELIKCLAAFINSGRHG 317

BLAST of PI0010812 vs. NCBI nr
Match: XP_022943297.1 (probable carboxylesterase 12 [Cucurbita moschata] >KAG6571161.1 putative carboxylesterase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 583.9 bits (1504), Expect = 8.1e-163
Identity = 269/318 (84.59%), Postives = 293/318 (92.14%), Query Frame = 0

Query: 1   MASDEIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYR 60
           MASDEIALN  PYF+VYKDGRIDR IGND DPPG+DPKTGVESKDV +SP+  V+VR+YR
Sbjct: 1   MASDEIALNAVPYFVVYKDGRIDRLIGNDFDPPGVDPKTGVESKDVVVSPEDSVSVRIYR 60

Query: 61  PKSAAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQ 120
           PKSAAE Q++KLPLLVYFHGGGFCIETAFSPFYHQHI+AWVA+ANI AVSVNYRRAPE+Q
Sbjct: 61  PKSAAE-QAQKLPLLVYFHGGGFCIETAFSPFYHQHINAWVAKANIAAVSVNYRRAPEYQ 120

Query: 121 LPIPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEG 180
           LPIPFED+WTAMKW+A+HSGG GPDEWLNEIADLN+VYLAGDSAGGN+AHRMALR  TEG
Sbjct: 121 LPIPFEDSWTAMKWVAAHSGGAGPDEWLNEIADLNRVYLAGDSAGGNIAHRMALRVATEG 180

Query: 181 LEGVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHD 240
           L GVKIKGLQLIHPHFWGGKLIGEE +WDPKD+FVVENLW+VVSKDIK LDDPIVNPEHD
Sbjct: 181 LAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKDIFVVENLWYVVSKDIKELDDPIVNPEHD 240

Query: 241 PDLGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTC 300
           PDL RLP E+V VYVAEKDNLKERGRHYAECL+KSGWGG VEVVET+GEGHVFHLFNPTC
Sbjct: 241 PDLARLPAEKVAVYVAEKDNLKERGRHYAECLRKSGWGGAVEVVETDGEGHVFHLFNPTC 300

Query: 301 DMAGELVKQLAAFIKSGC 317
           D A +LV+ LAAF  S C
Sbjct: 301 DSAADLVELLAAFTSSDC 317

BLAST of PI0010812 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 2.3e-83
Identity = 154/320 (48.12%), Postives = 206/320 (64.38%), Query Frame = 0

Query: 5   EIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYRPKSA 64
           EIA++ +P   +YK GRI+R +G    PP  +P+ GV SKDV  S D  ++VR+Y P+ A
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 65  AEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLPIP 124
           A +   KLPLLVYFHGGGF IETAFSP YH  ++  V+ +N  AVSV+YRRAPEH + +P
Sbjct: 64  AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 123

Query: 125 FEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTE----G 184
           F+D+WTA+KW+ +H  G G ++WLN+ AD ++V+L+GDSAG N+ H MA+R   E    G
Sbjct: 124 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSPG 183

Query: 185 LEGVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKT-LDDPIVN--P 244
           L    I G+ L+HP+FW    I E++  D      +E  W + S + K   DDP++N   
Sbjct: 184 LNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVVQ 243

Query: 245 EHDPDLGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFN 304
               DL  L   +V V VAEKD L  +G  YA  L+KSGW G VEVVE+EGE HVFHL  
Sbjct: 244 SESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLLK 303

Query: 305 PTCDMAGELVKQLAAFIKSG 316
           P CD A E++ + + FIK G
Sbjct: 304 PECDNAIEVMHKFSGFIKGG 323

BLAST of PI0010812 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 289.3 bits (739), Expect = 3.8e-78
Identity = 150/323 (46.44%), Postives = 205/323 (63.47%), Query Frame = 0

Query: 5   EIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYRPKSA 64
           EIA + +P  I+YK GRI+R +G    PP  +P+ GV SKDV  SPD  +++R+Y P+ A
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 65  AEKQSE---KLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQL 124
           A  ++E   KLPLLVYFHGGGF +ETAFSP YH  ++A V+ ++  AVSV+YRRAPEH +
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 125 PIPFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTV---- 184
           P  ++D+WTA+KW+ SH  G G ++WLN+ AD ++V+LAGDSAG N+ H M ++      
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 185 -TEGLEGVKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKT-LDDPIV 244
             E L    I G+ L+HP+FW    + ++   D      +E++W + S + K   DDP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 245 N--PEHDPDLGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTV-EVVETEGEGHV 304
           N       DL  L   +V V VAEKD L  +G  Y E L KS W G V +VVET+GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

Query: 305 FHLFNPTCDMAGELVKQLAAFIK 314
           FHL +P  + A ELV + A FIK
Sbjct: 304 FHLRDPNSEKAHELVHRFAGFIK 326

BLAST of PI0010812 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 285.0 bits (728), Expect = 7.2e-77
Identity = 151/310 (48.71%), Postives = 196/310 (63.23%), Query Frame = 0

Query: 6   IALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYRPKSAA 65
           IA + +P F VYK GRI+R +G    PP L P+ GV SKD+  SP+  +++R+Y P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  EKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLPIPF 125
            K   KLP+L+YFHGGGF IETAFSP YH  +++ VA AN  A+SVNYRRAPE  +PIP+
Sbjct: 65  VK---KLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPY 124

Query: 126 EDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEGVK 185
           ED+W ++KW+ +H  G GP+ W+N+  D  +V+LAGDSAGGN++H + +R   E L    
Sbjct: 125 EDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSL 184

Query: 186 IKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKT-LDDPIVNPEHDPDLG 245
           I G+ LIHP+FW    I E    D      VE  W V S + K  +DDP +N       G
Sbjct: 185 ISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDPSG 244

Query: 246 RLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDMAG 305
            L   RV V VA  D    +G  YAE LKKSGW G VEV+ET+ EGHVFHL NP  D A 
Sbjct: 245 -LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNAR 304

Query: 306 ELVKQLAAFI 313
           ++VK+L  FI
Sbjct: 305 QVVKKLEEFI 310

BLAST of PI0010812 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.3e-75
Identity = 144/312 (46.15%), Postives = 202/312 (64.74%), Query Frame = 0

Query: 5   EIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISP--DVAVRVYRPKSA 64
           +++L + P+ +V+ DG ++R  G ++ PPGLDP TGV SKD+ I P   ++ R+YRP S 
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLPIP 124
             +  +K+PL++YFHGG F I +   P YH  ++  V +AN+ AVSVNYR APEH LP  
Sbjct: 67  --QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTA 126

Query: 125 FEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEGV 184
           +ED+WTA+K I + +     + W+N+ ADL+ ++L GDSAG N++H +A R   +  + +
Sbjct: 127 YEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQTL 186

Query: 185 KIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVSKDIKTLDDPIVNPEHD--PD 244
           KIKG+ +IHP+FWG + IG E   D     +V+  W  V    K  DDP +NP  D  PD
Sbjct: 187 KIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPFADGSPD 246

Query: 245 LGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTCDM 304
           LG L  ERV + VAEKD L ERG+ Y E L KS W G VE++ET+ + HVFH+F P CD 
Sbjct: 247 LGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDCDE 306

Query: 305 AGELVKQLAAFI 313
           A E+V+ LA FI
Sbjct: 307 AMEMVRCLALFI 309

BLAST of PI0010812 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 269.6 bits (688), Expect = 3.1e-72
Identity = 139/314 (44.27%), Postives = 189/314 (60.19%), Query Frame = 0

Query: 5   EIALNVAPYFIVYKDGRIDRFIGNDIDPPGLDPKTGVESKDVDISPD--VAVRVYRP-KS 64
           EIA    P+  +YKDGR++R IG D  P  LDP   V SKDV  SP+  ++VR++ P KS
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKS 63

Query: 65  AAEKQSEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANITAVSVNYRRAPEHQLPI 124
                  KLPLL+Y HGG + IE+ FSP YH +++  V  AN  AVSV YRRAPE  +P 
Sbjct: 64  TKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVPA 123

Query: 125 PFEDAWTAMKWIASHSGGKGPDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVTEGLEG 184
            +ED W+A++WI +HS G GP +W+N+ AD  +V+L GDSAGGN++H MA++   E    
Sbjct: 124 AYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKLD 183

Query: 185 VKIKGLQLIHPHFWGGKLIGEENDWDPKDLFVVENLWFVVS--KDIKTLDDPIVNPE-HD 244
           +KIKG+ ++HP FWG   + E +  D +    +  +W  ++    +   DDP+ N     
Sbjct: 184 LKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGSG 243

Query: 245 PDLGRLPVERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNPTC 304
            D   L  ++V V VA KD    +G  YA  L+K  W GTVEVVE EGE HVFHL NP  
Sbjct: 244 SDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPKS 303

Query: 305 DMAGELVKQLAAFI 313
           D A + +K+   FI
Sbjct: 304 DKALKFLKKFVEFI 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMN03.3e-8248.13Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1[more]
Q9SMM95.4e-7746.44Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1[more]
Q9ZQ911.0e-7548.71Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1[more]
Q9SX783.3e-7446.15Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
Q9FX944.4e-7144.27Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LKY57.6e-18395.28Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G2345... [more]
A0A5A7SP214.2e-18195.28Putative carboxylesterase 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3CA697.1e-18194.97probable carboxylesterase 12 OS=Cucumis melo OX=3656 GN=LOC103498399 PE=4 SV=1[more]
A0A5D3CQ217.1e-18194.97Putative carboxylesterase 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1FSN23.9e-16384.59probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448111 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_004145409.11.6e-18295.28probable carboxylesterase 12 [Cucumis sativus] >KGN61724.1 hypothetical protein ... [more]
KAA0032083.18.6e-18195.28putative carboxylesterase 12 [Cucumis melo var. makuwa][more]
XP_008459207.11.5e-18094.97PREDICTED: probable carboxylesterase 12 [Cucumis melo] >XP_008459208.1 PREDICTED... [more]
XP_038900341.18.1e-17189.94probable carboxylesterase 7 [Benincasa hispida][more]
XP_022943297.18.1e-16384.59probable carboxylesterase 12 [Cucurbita moschata] >KAG6571161.1 putative carboxy... [more]
Match NameE-valueIdentityDescription
AT3G48690.12.3e-8348.13alpha/beta-Hydrolases superfamily protein [more]
AT3G48700.13.8e-7846.44carboxyesterase 13 [more]
AT2G03550.17.2e-7748.71alpha/beta-Hydrolases superfamily protein [more]
AT1G47480.12.3e-7546.15alpha/beta-Hydrolases superfamily protein [more]
AT1G49660.13.1e-7244.27carboxyesterase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 73..292
e-value: 7.3E-46
score: 156.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 7..316
e-value: 6.0E-67
score: 228.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 6..313
NoneNo IPR availablePANTHERPTHR23024:SF458CARBOXYLESTERASE 2-RELATEDcoord: 3..313
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 3..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0010812.1PI0010812.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity