PI0010724 (gene) Melon (PI 482460) v1

BLAST of PI0010724 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.1e-121
Identity = 231/499 (46.29%), Postives = 330/499 (66.13%), Query Frame = 0

Query: 2   MEMLSVCVIIGSVLGVYF----FVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSF 61
           ME+  VC+   ++L  Y     FVR LN  +Y VKL RR    LPPGD+G PLIG   SF
Sbjct: 1   MEVHWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKL-RRKEHPLPPGDMGWPLIGDLLSF 60

Query: 62  YRAFKAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYP 121
            + F + G PDSFI++L+ + GR G+YK+HL+G P+IIV +P++CRR+  DD  FK  YP
Sbjct: 61  IKDF-SSGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYP 120

Query: 122 KSVR-------ILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWS 181
           KS++       +++V    S  +H+   R+++SP+ G++ L+ Y+  ++EI+   LE+ S
Sbjct: 121 KSIKELARCRPMIDV----SNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELS 180

Query: 182 TMKEPIELLSEIGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLT 241
           +MK P+ELL E+  + FK I+ +F+G+      +  + + +  L   + SI P ++PG T
Sbjct: 181 SMKHPVELLKEMKKVSFKAIVHVFMGSS-NQDIIKKIGSSFTDLYNGMFSI-PINVPGFT 240

Query: 242 YHDALKARKEIGNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAI 301
           +H AL+ARK++  I++ V++E+R ++E         K  +D L+   DENG+KL D + I
Sbjct: 241 FHKALEARKKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDED-I 300

Query: 302 IDLLLGLLHAGHHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIK 361
            DLL+GLL AGH + A + MW+I  ++Q+ H+ +KAKEEQE I + R S+QK LS KEIK
Sbjct: 301 SDLLIGLLFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIK 360

Query: 362 QMKYLMKFINELLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNP 421
           QM YL + I+E LR   IA A+FREA  +V IN Y IPKGW V IW RAIHMD + Y NP
Sbjct: 361 QMVYLSQVIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNP 420

Query: 422 QEFDPSRWDNYTPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDT 481
           +EF+PSRWD+Y  K G FLPFG GSR CPG++L KL I+I LH+F+ NY++ER+NP C  
Sbjct: 421 EEFNPSRWDDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHV 480

Query: 482 THLPAPKLVDNCLCRIIKL 490
           T LP  K  DNCL ++IK+
Sbjct: 481 TSLPVSKPTDNCLAKVIKV 490

BLAST of PI0010724 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 401.4 bits (1030), Expect = 1.5e-110
Identity = 222/484 (45.87%), Postives = 309/484 (63.84%), Query Frame = 0

Query: 9   VIIGSVLGVYFFVRKLNEIW-YLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAFKAGGDP 68
           +I+G  L V  +V K   +W Y+ KLG + +  LPPGDLG P+IG+ +SF RAFK   DP
Sbjct: 14  IILG--LFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 69  DSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVRILEVNG 128
           +SFI   I+R GR G+YK+H++G P ++VT PE CRR+  DD+ F   +PKS   L    
Sbjct: 74  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 133

Query: 129 LFSRI---DHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSEIGS 188
            F  I   +HK   R+ S+P+NG E LS Y+ FI+E +   LE WS M E IE LS +  
Sbjct: 134 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 193

Query: 189 LLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEIGNI 248
           L FKVI+ IFL +E     ++ LE  Y +L   + ++   +LPG  YH ALKARK++   
Sbjct: 194 LTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAA 253

Query: 249 LECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAGHHT 308
            + ++  +R   +RK+      K  +D LI   DENG+ L D+  IIDLLL  L+AGH +
Sbjct: 254 FQSIVTNRRN--QRKQNISSNRKDMLDNLIDVKDENGRVL-DDEEIIDLLLMYLNAGHES 313

Query: 309 PAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINELLR 368
             +  MWA + + ++  + QKAKEEQE I+K+R   QK L+ KE ++M YL + I+E LR
Sbjct: 314 SGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLR 373

Query: 369 RHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNYTPK 428
             T ++ +FREAK++V ++ Y IPKGW V  W R +H+DP+IY +P++FDPSRW+ YTPK
Sbjct: 374 VITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPK 433

Query: 429 PGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDNCLC 488
            G FLPFG+GS  CPG++L KL I+I LHHF+L Y++ER NP C    LP  +  DNCL 
Sbjct: 434 AGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLA 486

BLAST of PI0010724 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 4.1e-108
Identity = 209/475 (44.00%), Postives = 299/475 (62.95%), Query Frame = 0

Query: 16  GVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAFKAGGDPDSFIHHLI 75
           G+ + VRK+N   Y   LG   +  LPPGDLG P IG+  SF RAFK   DPDSF   LI
Sbjct: 21  GLNWLVRKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLI 80

Query: 76  SRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVRILEVNGLFSRI--- 135
            R G  G+YK+H++G P+IIVT  + CRR+  DD+ FKP +P S   L     F  I   
Sbjct: 81  KRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFE 140

Query: 136 DHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSEIGSLLFKVIIR 195
           +HK   R+ ++P+NG+E LS Y+ +I+E +   L+ W+ M E  E L+ +  L F++I+ 
Sbjct: 141 EHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMY 200

Query: 196 IFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEIGNILECVIEEK 255
           IFL +E     ++ LE  Y  L   + ++   ++PG  YH ALKARK +    + ++ E+
Sbjct: 201 IFLSSESEN-VMDALEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQSIVTER 260

Query: 256 RRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAGHHTPAYAAMWA 315
           R   +RK+      K  +D L+   DE+GK L D+  IID+LL  L+AGH +  +  MWA
Sbjct: 261 RN--QRKQNILSNKKDMLDNLLNVKDEDGKTL-DDEEIIDVLLMYLNAGHESSGHTIMWA 320

Query: 316 IVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINELLRRHTIAVAS 375
            V + ++  V Q+AK EQE I+K RP  QKGLS KE ++M++L + ++E LR  T ++ +
Sbjct: 321 TVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTA 380

Query: 376 FREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDN-YTPKPGEFLPF 435
           FREAKT+V +N Y IPKGW V  W R +H+DP+++ +P++FDP+RWDN + PK G FLPF
Sbjct: 381 FREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPF 440

Query: 436 GIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDNCLCRI 487
           G GS  CPG++L KL I+I LHHF+L Y+++R NP C   +LP  +  DNCL RI
Sbjct: 441 GAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of PI0010724 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 3.6e-104
Identity = 192/490 (39.18%), Postives = 311/490 (63.47%), Query Frame = 0

Query: 4   MLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAFKA 63
           +L+  V+ G VL V   VR+ ++   +  LG      LPPG++G P++GS ++F RAFK+
Sbjct: 19  VLAAAVVAGVVL-VDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKS 78

Query: 64  GGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVRIL 123
            G+PD+FI   I R GR G+Y++ ++  P I+   PE C+++ +DDE F   +PK+   L
Sbjct: 79  -GNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTL 138

Query: 124 EVNGLF---SRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKE-PIELL 183
                F   S  DH+   ++ ++P+NG++ L+ Y+SFID+ +   L  WS+ +   +E L
Sbjct: 139 IGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFL 198

Query: 184 SEIGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARK 243
           +E+  + FK+I++IF+       T+  LE  Y  L   + ++   +LPG  Y+ AL+AR+
Sbjct: 199 TELRRMTFKIIVQIFMSG-ADDATMEALERSYTDLNYGMRAM-AINLPGFAYYRALRARR 258

Query: 244 EIGNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLH 303
           ++ ++L+ V++ +R    +   R   +   MD+LI A DE G++L D+  I+D+L+  L+
Sbjct: 259 KLVSVLQGVLDGRRAAAAKGFKRSGAMD-MMDRLIEAEDERGRRLADDE-IVDVLIMYLN 318

Query: 304 AGHHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFI 363
           AGH +  +  MWA V + +N  +F +AK EQE IM+  P+TQ GL+ ++ K+M +L + +
Sbjct: 319 AGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVV 378

Query: 364 NELLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWD 423
           +E LR   I+  SFR+A  ++++N Y IPKGW V +W R++HMD Q+Y +P+ F+PSRW+
Sbjct: 379 DETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWE 438

Query: 424 NYTPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLV 483
              PK G FLPFG+G+R CPG++L KL I++ LHHF+L YK++R NP+C   +LP P+ V
Sbjct: 439 GPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPV 498

Query: 484 DNCLCRIIKL 490
           DNCL  I K+
Sbjct: 499 DNCLATITKV 502

BLAST of PI0010724 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 4.7e-104
Identity = 187/466 (40.13%), Postives = 296/466 (63.52%), Query Frame = 0

Query: 28  WYL-VKLGRRVYKSLPPGDLGLPLIGSSFSFYRAFKAGGDPDSFIHHLISRNGRVGMYKS 87
           WY    LG      LPPG++G PL+G  ++F RAFK+ G PD+FI   + R GR G+Y+S
Sbjct: 58  WYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKS-GKPDAFIASFVRRFGRTGVYRS 117

Query: 88  HLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVRILEVNGLFSRI---DHKTAYRIMSS 147
            ++  P ++VT  E C+++ +DD+ F   +PK+   L     F  +   +H+   ++ ++
Sbjct: 118 FMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRIRKLTAA 177

Query: 148 PLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSEIGSLLFKVIIRIFLGNEIPTPT 207
           P+NG++ L+ Y+ FID  +T  L  W+     +E L+E+  + FK+I++IFLG      T
Sbjct: 178 PINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGG-ADQAT 237

Query: 208 LNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEIGNILECVIEEKRRVLERKETRD 267
              LE  Y  L   + ++   +LPG  Y  AL+AR+ +  +L+ V++E+R    +  +  
Sbjct: 238 TRALERSYTELNYGMRAM-AINLPGFAYRGALRARRRLVAVLQGVLDERRAARAKGVSGG 297

Query: 268 QLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAGHHTPAYAAMWAIVQISQNSHVF 327
            +    MD+LI A DE G+ L D++ IID+L+  L+AGH +  +  MWA V + +N  +F
Sbjct: 298 GV--DMMDRLIEAQDERGRHL-DDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPDMF 357

Query: 328 QKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINELLRRHTIAVASFREAKTNVYIN 387
            +AK EQE IM+  PS+Q+GL+ ++ ++M+YL + I+E LR   I+  SFR+A  +V++N
Sbjct: 358 ARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQATRDVFVN 417

Query: 388 DYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNYTPKPGEFLPFGIGSRFCPGSEL 447
            Y IPKGW V +W R++HMDPQ+Y +P +FDPSRW+ ++P+ G FL FG+G+R CPG++L
Sbjct: 418 GYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGARLCPGNDL 477

Query: 448 TKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDNCLCRIIKL 490
            KL I++ LHHF+L YK+ R NPRC   +LP P+ VDNCL +I ++
Sbjct: 478 AKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAKITRV 517

BLAST of PI0010724 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.2e-231
Identity = 391/489 (79.96%), Postives = 433/489 (88.55%), Query Frame = 0

Query: 2   MEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAF 61
           ME ++VCVIIG VLGVY FVRKLNEIWYLVKLGR+ YKSLPPGD+G PL+GSSFSFY AF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60

Query: 62  KAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVR 121
             GGDPDSFI+HL+SRNGRVGMYKSH YGRP IIVTDPEICRRIYLDDE FKP+YPKSV+
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 122 ILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSE 181
           ILEVNG F+R DHK+AYRIM++P+NGYE+LS YV FID++M KGLE+WS+MKEPIELL E
Sbjct: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180

Query: 182 IGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEI 241
           IGSL FKVI RIFLG +I    +  LE LYK LGPAILSI PYDLPGLT+H ALKARK+I
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240

Query: 242 GNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAG 301
            NIL+CVIEEKRR LE++E     V+ QMDKLI+A DENG KLYDNNAIIDLLLGLLHAG
Sbjct: 241 ENILQCVIEEKRRALEKEERE---VESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAG 300

Query: 302 HHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE 361
           HHTPAYAAMWA+V IS+  HVF KAKEEQE I+K+RPSTQKGL+F+EIKQMKYLMKFINE
Sbjct: 301 HHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINE 360

Query: 362 LLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNY 421
           LLRR+TIAVASFR+AK +V INDY IPKGW V IWTRAIHMDPQ+YSNPQEFDPSRW+NY
Sbjct: 361 LLRRNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWENY 420

Query: 422 TPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDN 481
           +PKPG FLPFGIG+RFCPGSELTKL ITILLHHFILNYKMERVNP+C  THLPAPKLVDN
Sbjct: 421 SPKPGTFLPFGIGTRFCPGSELTKLEITILLHHFILNYKMERVNPKCCITHLPAPKLVDN 480

Query: 482 CLCRIIKLP 491
           CLC I KLP
Sbjct: 481 CLCTITKLP 486

BLAST of PI0010724 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.2e-231
Identity = 385/489 (78.73%), Postives = 435/489 (88.96%), Query Frame = 0

Query: 2   MEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAF 61
           MEM++VC IIG+VLGVY FVRKLNEIWYLVKLGR+ YKSLPPGD+G PL+GSSFSFY AF
Sbjct: 1   MEMINVCTIIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60

Query: 62  KAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVR 121
             GGDPDSFI+HL+SRNGRVGMYKSHLYGRP IIVTDPEICRRIYLDDE FKP+YPKSV+
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 122 ILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSE 181
           ILEVNG F+R DHK+AYRIM++P+NGYE+LS YV FID++M KGLE+WS+MKEPIELL E
Sbjct: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180

Query: 182 IGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEI 241
           IGSL FKVI RIFLG +I    +  LE LYK LGPAILSI PYD+PGL YH ALKARK+I
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKI 240

Query: 242 GNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAG 301
            N+LECVIEEKRR LE+KETR+  V  QMDKLI+A DENG KLYDNN IIDLLLGLLHAG
Sbjct: 241 ENVLECVIEEKRRALEQKETRE--VHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAG 300

Query: 302 HHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE 361
           HHTPAYAAMWA+V IS+  H+F KAKEEQE I+K+RPSTQKGL+F+EIKQMKYLMKFINE
Sbjct: 301 HHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINE 360

Query: 362 LLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNY 421
           LLRR+TI VASFR+AKT++ INDY IPKGW+V IWTRA+HMDPQ+YSNP+EFDPSRW+N+
Sbjct: 361 LLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWENH 420

Query: 422 TPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDN 481
           +PKPG F+PFGIG+RFCPGSELTKL ITILLHHFILNYKMER NP+C  +HLPAPKLVDN
Sbjct: 421 SPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYKMERTNPKCCISHLPAPKLVDN 480

Query: 482 CLCRIIKLP 491
           CLC I KLP
Sbjct: 481 CLCTITKLP 487

BLAST of PI0010724 vs. ExPASy TrEMBL
Match: A0A1S4E5L3 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103503515 PE=3 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 9.8e-230
Identity = 399/463 (86.18%), Postives = 425/463 (91.79%), Query Frame = 0

Query: 1   MMEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRA 60
           MMEMLSVCVIIGSVLGVYFFVRKLNEIW+LVKLGRRVYKSLPPGDLG PLIGSSFSFY+A
Sbjct: 1   MMEMLSVCVIIGSVLGVYFFVRKLNEIWHLVKLGRRVYKSLPPGDLGWPLIGSSFSFYKA 60

Query: 61  FKAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSV 120
           FKAGGDPDSFIH LISRNG                       RRIYLDDEKFKP+YPKS+
Sbjct: 61  FKAGGDPDSFIHRLISRNG-----------------------RRIYLDDEKFKPNYPKSI 120

Query: 121 RILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLS 180
           ++LEVNGLFS +DHKTAY+IM+SPLNGYEILSKYV+FID++M+KGLE+WSTMKEPIELLS
Sbjct: 121 KLLEVNGLFSSMDHKTAYKIMASPLNGYEILSKYVTFIDKLMSKGLEEWSTMKEPIELLS 180

Query: 181 EIGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKE 240
           EIGS+LFKVII IFLGNEIPT TLN LEALYKHLGPAILSI PYDLPGLTYH ALKARKE
Sbjct: 181 EIGSILFKVIISIFLGNEIPTTTLNKLEALYKHLGPAILSILPYDLPGLTYHHALKARKE 240

Query: 241 IGNILECVIEEKRRVLER-KETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLH 300
           IGNILECVIEEKRRVLER KET+DQLV+CQMDKLIVA+DENGK+LYDNNAIIDLLLGLLH
Sbjct: 241 IGNILECVIEEKRRVLERNKETKDQLVQCQMDKLIVAIDENGKRLYDNNAIIDLLLGLLH 300

Query: 301 AGHHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFI 360
           AGHHTPAYAA WA+VQISQNSHVFQKAKEEQEFIMKERPS+QKGLSFKEIKQMKYLMKF+
Sbjct: 301 AGHHTPAYAATWAVVQISQNSHVFQKAKEEQEFIMKERPSSQKGLSFKEIKQMKYLMKFV 360

Query: 361 NELLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWD 420
           NELLRR+ IAVASFREA TNVYINDYTIPKGW V IWTRAIHMDPQ+YSNPQEFDPSRWD
Sbjct: 361 NELLRRYAIAVASFREATTNVYINDYTIPKGWIVTIWTRAIHMDPQMYSNPQEFDPSRWD 420

Query: 421 NYTPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKME 463
           NYTPKPGEF+PFG+GSRFCPGSELTKL ITILLHHFILNYKME
Sbjct: 421 NYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFILNYKME 440

BLAST of PI0010724 vs. ExPASy TrEMBL
Match: A0A6J1GJP8 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.9e-229
Identity = 386/489 (78.94%), Postives = 432/489 (88.34%), Query Frame = 0

Query: 2   MEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAF 61
           ME ++VC+IIG +LGVY FVRKLNEIWYLVKLGR+ YKSLPPGD+G PL+GSSFSFY+AF
Sbjct: 1   METINVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60

Query: 62  KAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVR 121
             GGDPDSFI+HL+SRNGRVGMYKSHLYG P IIVTDPEICRRIYLDDE FKP+YPKSV+
Sbjct: 61  IVGGDPDSFIYHLLSRNGRVGMYKSHLYGSPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 122 ILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSE 181
           ILEVNGLFSR+DHK AYRIM++P+NGY +LS YV FID+IM KGLE+WST KEPIEL  E
Sbjct: 121 ILEVNGLFSRMDHKCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEEWSTKKEPIELFGE 180

Query: 182 IGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEI 241
           IGSL F+VI RIFLG +I    +  LE LYK LGPAILSI PYDLPGLT+H ALKARK+I
Sbjct: 181 IGSLFFRVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHRALKARKKI 240

Query: 242 GNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAG 301
            NIL+CVIEEKRR LE++E     V+ QMDKLI+A DENG KLYDNNAIIDLLLGLLH G
Sbjct: 241 ENILQCVIEEKRRALEKQERE---VESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHGG 300

Query: 302 HHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE 361
           HHTPAYAAMWA+V IS+  HVF KAKEEQE I+K+R STQKGL+F+EIKQMKYLMKFINE
Sbjct: 301 HHTPAYAAMWALVHISETPHVFHKAKEEQESILKQRSSTQKGLTFQEIKQMKYLMKFINE 360

Query: 362 LLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNY 421
           LLRR+TIAVASFREAKT + INDYTIPKGWTV IWTRAIHMDPQ+YSNP+EFDPSRW+N+
Sbjct: 361 LLRRNTIAVASFREAKTEININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDPSRWENH 420

Query: 422 TPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDN 481
           +PKPG F+PFGIG+RFCPGSELTKL +TILLHHFILNYKMERVNP+C  THLPAPKLVDN
Sbjct: 421 SPKPGTFIPFGIGTRFCPGSELTKLELTILLHHFILNYKMERVNPKCCITHLPAPKLVDN 480

Query: 482 CLCRIIKLP 491
           CLC I KLP
Sbjct: 481 CLCTITKLP 486

BLAST of PI0010724 vs. ExPASy TrEMBL
Match: A0A6J1I8I5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.7e-227
Identity = 387/498 (77.71%), Postives = 434/498 (87.15%), Query Frame = 0

Query: 2   MEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAF 61
           ME ++VCVIIG VLGVY FVRKLNEIWYLVKLGR+ YKSLPPGD+G PL+GSSFSFY+AF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60

Query: 62  KAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVR 121
             GGDPDSFI+HL+SRNGRVGMYKSHLYG+P IIVTDPEICRRIYLDDE F+P+YPKSV+
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHLYGKPTIIVTDPEICRRIYLDDEIFQPNYPKSVK 120

Query: 122 ILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSE 181
           ILEVNGLFSR+DH  AYRIM++P+NGY +LS YV FID+IM KGLE WST KEPIELL E
Sbjct: 121 ILEVNGLFSRMDHNCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEKWSTKKEPIELLGE 180

Query: 182 IGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEI 241
           IGSL FKVI RIFLG +I    +  LE LYK LGPAILSI PYD+PGLT+H ALKARK+I
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLTFHRALKARKKI 240

Query: 242 GNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAG 301
            NIL+CVIEEKRR LE++E     V+ QMDKLI+A DENG KLYDNNAIIDLLLGLLH G
Sbjct: 241 ENILQCVIEEKRRALEKQERE---VESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHGG 300

Query: 302 HHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE 361
           HHTPAYAAMWA+VQI +  HVF KAKEEQE I+K+RPSTQKGL+F+EIKQMKYLMKFINE
Sbjct: 301 HHTPAYAAMWALVQIFETPHVFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINE 360

Query: 362 LLRRHTIAVASFREAKTNVYIN---------DYTIPKGWTVPIWTRAIHMDPQIYSNPQE 421
           LLRR+TIAVASFREAKT++ IN         DYTIPKGWTV IWTRAIHMDPQ+YSNP+E
Sbjct: 361 LLRRNTIAVASFREAKTDININGELFYYIVLDYTIPKGWTVLIWTRAIHMDPQLYSNPKE 420

Query: 422 FDPSRWDNYTPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTH 481
           FDPSRW+N++PKPG F+PFG+G+RFCPGSELTKL +TILLHHFILNYKMERVNP+C  TH
Sbjct: 421 FDPSRWENHSPKPGTFIPFGMGTRFCPGSELTKLELTILLHHFILNYKMERVNPKCCITH 480

Query: 482 LPAPKLVDNCLCRIIKLP 491
           LPAPKLVDNCLC I KLP
Sbjct: 481 LPAPKLVDNCLCTITKLP 495

BLAST of PI0010724 vs. NCBI nr
Match: XP_011652661.1 (beta-amyrin 11-oxidase [Cucumis sativus])

HSP 1 Score: 927.2 bits (2395), Expect = 6.0e-266
Identity = 452/491 (92.06%), Postives = 467/491 (95.11%), Query Frame = 0

Query: 1   MMEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRA 60
           MMEMLSVCVIIGSVLGVYFFVRKLNE+WYLVKLGRRVYKSLP GDLG PLIGSSFSFY+A
Sbjct: 1   MMEMLSVCVIIGSVLGVYFFVRKLNEVWYLVKLGRRVYKSLPSGDLGWPLIGSSFSFYKA 60

Query: 61  FKAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSV 120
           FKAGGDPDSFIHHLISRNGRVGMYKSHLYGRP IIVTDPEICRRIYLDD+KFKP+YPKSV
Sbjct: 61  FKAGGDPDSFIHHLISRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDQKFKPNYPKSV 120

Query: 121 RILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLS 180
           ++LEVNGLFSRIDHKT YRIM+SPLNGYEILSKYVSFI+EIM+KGLE+WSTMKEPIELLS
Sbjct: 121 KLLEVNGLFSRIDHKTFYRIMASPLNGYEILSKYVSFIEEIMSKGLEEWSTMKEPIELLS 180

Query: 181 EIGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKE 240
           EIGSLLFKVIIRIFLGNEIPTPTLN LEALYKHLGP ILSI PYD+PGLTYH ALKARKE
Sbjct: 181 EIGSLLFKVIIRIFLGNEIPTPTLNKLEALYKHLGPVILSILPYDVPGLTYHHALKARKE 240

Query: 241 IGNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHA 300
           I NILE VI EKRRVLERKET D+LVKCQMDKLIVA DENGKKLYDN AIIDLLLGLLHA
Sbjct: 241 IENILEGVIMEKRRVLERKETNDELVKCQMDKLIVATDENGKKLYDNKAIIDLLLGLLHA 300

Query: 301 GHHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFIN 360
           GHHTPAY AMWAIVQISQNSH+FQKAKEEQE IMKERPSTQKGLSF EIKQMKYLMKF N
Sbjct: 301 GHHTPAYTAMWAIVQISQNSHIFQKAKEEQELIMKERPSTQKGLSFTEIKQMKYLMKFTN 360

Query: 361 ELLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDN 420
           ELLRRHT+AVASFREA  NV+INDYTIPKGWTV IWTRAIHMDPQIYSNPQEFDPSRWDN
Sbjct: 361 ELLRRHTVAVASFREATANVHINDYTIPKGWTVTIWTRAIHMDPQIYSNPQEFDPSRWDN 420

Query: 421 YTPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVD 480
           YTPKPGEFLPFGIGSRFCPGSELTKLYITILLH FILNYKMERVN RCDTTHLPAPKLVD
Sbjct: 421 YTPKPGEFLPFGIGSRFCPGSELTKLYITILLHQFILNYKMERVNQRCDTTHLPAPKLVD 480

Query: 481 NCLCRIIKLPY 492
           NCLCRIIKL Y
Sbjct: 481 NCLCRIIKLSY 491

BLAST of PI0010724 vs. NCBI nr
Match: XP_038877829.1 (beta-amyrin 11-oxidase-like [Benincasa hispida])

HSP 1 Score: 877.9 bits (2267), Expect = 4.2e-251
Identity = 430/489 (87.93%), Postives = 452/489 (92.43%), Query Frame = 0

Query: 2   MEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAF 61
           ME++S  VIIGSVL VY FVRK NEIWYLVKLGR+VYKSLPPGD G P IG S SFY+AF
Sbjct: 1   MEIISAYVIIGSVLWVYLFVRKFNEIWYLVKLGRKVYKSLPPGDFGWPFIGYSLSFYKAF 60

Query: 62  KAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVR 121
           KAGGDPDSFI HL+S+NGRVGMYKSHLYGRP IIVTD EICR IYLDDEKFKP+YPKSV+
Sbjct: 61  KAGGDPDSFISHLLSKNGRVGMYKSHLYGRPTIIVTDLEICRLIYLDDEKFKPNYPKSVK 120

Query: 122 ILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSE 181
           ILEVNGLFSRIDHKTAY+IM+SP+NGYEILS YVSFID+ M KGLE+WST KEPIELLSE
Sbjct: 121 ILEVNGLFSRIDHKTAYKIMASPINGYEILSNYVSFIDQTMAKGLEEWSTAKEPIELLSE 180

Query: 182 IGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEI 241
           IGSLLFKVII IFLGNEI  PT+  LE LYKHLGPAILSI PYDLPGLT+H ALKARKEI
Sbjct: 181 IGSLLFKVIIHIFLGNEITAPTMAKLEELYKHLGPAILSILPYDLPGLTFHHALKARKEI 240

Query: 242 GNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAG 301
           GNIL+CVIEEKRRVLE KETR+  VK QMDKLIVA DENGKKLYDNN IIDLLLGLLHAG
Sbjct: 241 GNILDCVIEEKRRVLENKETRE--VKSQMDKLIVATDENGKKLYDNNDIIDLLLGLLHAG 300

Query: 302 HHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE 361
           HHTPAYAAMWA+VQIS+N+HVFQ  KEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE
Sbjct: 301 HHTPAYAAMWALVQISENAHVFQ--KEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE 360

Query: 362 LLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNY 421
           LLRRHTIAVASFREAKT+VYINDY IPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNY
Sbjct: 361 LLRRHTIAVASFREAKTDVYINDYIIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNY 420

Query: 422 TPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDN 481
           TPKPG F+PFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCD THLPAPKLVDN
Sbjct: 421 TPKPGAFIPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDITHLPAPKLVDN 480

Query: 482 CLCRIIKLP 491
           CLCRIIKLP
Sbjct: 481 CLCRIIKLP 485

BLAST of PI0010724 vs. NCBI nr
Match: XP_022952147.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 810.1 bits (2091), Expect = 1.1e-230
Identity = 385/489 (78.73%), Postives = 435/489 (88.96%), Query Frame = 0

Query: 2   MEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAF 61
           MEM++VC IIG+VLGVY FVRKLNEIWYLVKLGR+ YKSLPPGD+G PL+GSSFSFY AF
Sbjct: 1   MEMINVCTIIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60

Query: 62  KAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVR 121
             GGDPDSFI+HL+SRNGRVGMYKSHLYGRP IIVTDPEICRRIYLDDE FKP+YPKSV+
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 122 ILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSE 181
           ILEVNG F+R DHK+AYRIM++P+NGYE+LS YV FID++M KGLE+WS+MKEPIELL E
Sbjct: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180

Query: 182 IGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEI 241
           IGSL FKVI RIFLG +I    +  LE LYK LGPAILSI PYD+PGL YH ALKARK+I
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALKARKKI 240

Query: 242 GNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAG 301
            N+LECVIEEKRR LE+KETR+  V  QMDKLI+A DENG KLYDNN IIDLLLGLLHAG
Sbjct: 241 ENVLECVIEEKRRALEQKETRE--VHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHAG 300

Query: 302 HHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE 361
           HHTPAYAAMWA+V IS+  H+F KAKEEQE I+K+RPSTQKGL+F+EIKQMKYLMKFINE
Sbjct: 301 HHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFINE 360

Query: 362 LLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNY 421
           LLRR+TI VASFR+AKT++ INDY IPKGW+V IWTRA+HMDPQ+YSNP+EFDPSRW+N+
Sbjct: 361 LLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWENH 420

Query: 422 TPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDN 481
           +PKPG F+PFGIG+RFCPGSELTKL ITILLHHFILNYKMER NP+C  +HLPAPKLVDN
Sbjct: 421 SPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYKMERTNPKCCISHLPAPKLVDN 480

Query: 482 CLCRIIKLP 491
           CLC I KLP
Sbjct: 481 CLCTITKLP 487

BLAST of PI0010724 vs. NCBI nr
Match: XP_022952843.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 810.1 bits (2091), Expect = 1.1e-230
Identity = 391/489 (79.96%), Postives = 433/489 (88.55%), Query Frame = 0

Query: 2   MEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAF 61
           ME ++VCVIIG VLGVY FVRKLNEIWYLVKLGR+ YKSLPPGD+G PL+GSSFSFY AF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60

Query: 62  KAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVR 121
             GGDPDSFI+HL+SRNGRVGMYKSH YGRP IIVTDPEICRRIYLDDE FKP+YPKSV+
Sbjct: 61  TVGGDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120

Query: 122 ILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSE 181
           ILEVNG F+R DHK+AYRIM++P+NGYE+LS YV FID++M KGLE+WS+MKEPIELL E
Sbjct: 121 ILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPIELLGE 180

Query: 182 IGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEI 241
           IGSL FKVI RIFLG +I    +  LE LYK LGPAILSI PYDLPGLT+H ALKARK+I
Sbjct: 181 IGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDLPGLTFHGALKARKKI 240

Query: 242 GNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAG 301
            NIL+CVIEEKRR LE++E     V+ QMDKLI+A DENG KLYDNNAIIDLLLGLLHAG
Sbjct: 241 ENILQCVIEEKRRALEKEERE---VESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHAG 300

Query: 302 HHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE 361
           HHTPAYAAMWA+V IS+  HVF KAKEEQE I+K+RPSTQKGL+F+EIKQMKYLMKFINE
Sbjct: 301 HHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFINE 360

Query: 362 LLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNY 421
           LLRR+TIAVASFR+AK +V INDY IPKGW V IWTRAIHMDPQ+YSNPQEFDPSRW+NY
Sbjct: 361 LLRRNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWENY 420

Query: 422 TPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDN 481
           +PKPG FLPFGIG+RFCPGSELTKL ITILLHHFILNYKMERVNP+C  THLPAPKLVDN
Sbjct: 421 SPKPGTFLPFGIGTRFCPGSELTKLEITILLHHFILNYKMERVNPKCCITHLPAPKLVDN 480

Query: 482 CLCRIIKLP 491
           CLC I KLP
Sbjct: 481 CLCTITKLP 486

BLAST of PI0010724 vs. NCBI nr
Match: XP_023511599.1 (beta-amyrin 11-oxidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 805.8 bits (2080), Expect = 2.0e-229
Identity = 386/489 (78.94%), Postives = 435/489 (88.96%), Query Frame = 0

Query: 2   MEMLSVCVIIGSVLGVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAF 61
           ME ++VCVIIG VLGVY FVRKLNEIWYLVKLGR+ YKSLPPGD+G PL+GSSFSFY+AF
Sbjct: 1   METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60

Query: 62  KAGGDPDSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVR 121
             GGDPDSFI+HL+SRNGRVGMYKSHLYG P IIVTDPEICRRIYLDDE F+P+YPKSV+
Sbjct: 61  TVGGDPDSFINHLLSRNGRVGMYKSHLYGSPTIIVTDPEICRRIYLDDEIFRPNYPKSVK 120

Query: 122 ILEVNGLFSRIDHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSE 181
           ILEVNGLFSR+DHK AYRIM++P+NGY +LS YV FID+IM KGLE+WST KEPIEL  E
Sbjct: 121 ILEVNGLFSRMDHKCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEEWSTKKEPIELFGE 180

Query: 182 IGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEI 241
           IGSL F+VI RIFLG +I    +  LE LYK LGPAILSI PYDLPGLT+H ALKARK+I
Sbjct: 181 IGSLFFRVISRIFLGTDIGPSRMAELETLYKDLGPAILSILPYDLPGLTFHRALKARKKI 240

Query: 242 GNILECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAG 301
            NIL+CVIEEKRR LE++E     V+ QMDKLI+A DENG KLYDNNAIIDLLLGLLH G
Sbjct: 241 ENILQCVIEEKRRALEKQERE---VESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHGG 300

Query: 302 HHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINE 361
           HHTPAYAAMWA+V IS+N H+F +AKEEQE I+K+RPSTQKGL+F++IKQMKYLMKFINE
Sbjct: 301 HHTPAYAAMWALVHISENPHIFYQAKEEQESILKQRPSTQKGLTFQKIKQMKYLMKFINE 360

Query: 362 LLRRHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNY 421
           LLRR+TIAVASFREAKT++ INDYTIPKGWTV IWTRAIHMDPQ+YSNP+EFDPSRW+N+
Sbjct: 361 LLRRNTIAVASFREAKTDININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDPSRWENH 420

Query: 422 TPKPGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDN 481
           +PKPG F+PFGIG+RFCPGSELTKL +TILLHHFILNYKMERVNP+C  THLPAPKLVDN
Sbjct: 421 SPKPGTFIPFGIGTRFCPGSELTKLELTILLHHFILNYKMERVNPKCCITHLPAPKLVDN 480

Query: 482 CLCRIIKLP 491
           CLC I KLP
Sbjct: 481 CLCTITKLP 486

BLAST of PI0010724 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-111
Identity = 222/484 (45.87%), Postives = 309/484 (63.84%), Query Frame = 0

Query: 9   VIIGSVLGVYFFVRKLNEIW-YLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAFKAGGDP 68
           +I+G  L V  +V K   +W Y+ KLG + +  LPPGDLG P+IG+ +SF RAFK   DP
Sbjct: 14  IILG--LFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 69  DSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVRILEVNG 128
           +SFI   I+R GR G+YK+H++G P ++VT PE CRR+  DD+ F   +PKS   L    
Sbjct: 74  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 133

Query: 129 LFSRI---DHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSEIGS 188
            F  I   +HK   R+ S+P+NG E LS Y+ FI+E +   LE WS M E IE LS +  
Sbjct: 134 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 193

Query: 189 LLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEIGNI 248
           L FKVI+ IFL +E     ++ LE  Y +L   + ++   +LPG  YH ALKARK++   
Sbjct: 194 LTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAA 253

Query: 249 LECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAGHHT 308
            + ++  +R   +RK+      K  +D LI   DENG+ L D+  IIDLLL  L+AGH +
Sbjct: 254 FQSIVTNRRN--QRKQNISSNRKDMLDNLIDVKDENGRVL-DDEEIIDLLLMYLNAGHES 313

Query: 309 PAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINELLR 368
             +  MWA + + ++  + QKAKEEQE I+K+R   QK L+ KE ++M YL + I+E LR
Sbjct: 314 SGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLR 373

Query: 369 RHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNYTPK 428
             T ++ +FREAK++V ++ Y IPKGW V  W R +H+DP+IY +P++FDPSRW+ YTPK
Sbjct: 374 VITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPK 433

Query: 429 PGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDNCLC 488
            G FLPFG+GS  CPG++L KL I+I LHHF+L Y++ER NP C    LP  +  DNCL 
Sbjct: 434 AGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLA 486

BLAST of PI0010724 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 401.4 bits (1030), Expect = 1.1e-111
Identity = 222/484 (45.87%), Postives = 309/484 (63.84%), Query Frame = 0

Query: 9   VIIGSVLGVYFFVRKLNEIW-YLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAFKAGGDP 68
           +I+G  L V  +V K   +W Y+ KLG + +  LPPGDLG P+IG+ +SF RAFK   DP
Sbjct: 14  IILG--LFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDP 73

Query: 69  DSFIHHLISRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVRILEVNG 128
           +SFI   I+R GR G+YK+H++G P ++VT PE CRR+  DD+ F   +PKS   L    
Sbjct: 74  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 133

Query: 129 LFSRI---DHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSEIGS 188
            F  I   +HK   R+ S+P+NG E LS Y+ FI+E +   LE WS M E IE LS +  
Sbjct: 134 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRK 193

Query: 189 LLFKVIIRIFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEIGNI 248
           L FKVI+ IFL +E     ++ LE  Y +L   + ++   +LPG  YH ALKARK++   
Sbjct: 194 LTFKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAA 253

Query: 249 LECVIEEKRRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAGHHT 308
            + ++  +R   +RK+      K  +D LI   DENG+ L D+  IIDLLL  L+AGH +
Sbjct: 254 FQSIVTNRRN--QRKQNISSNRKDMLDNLIDVKDENGRVL-DDEEIIDLLLMYLNAGHES 313

Query: 309 PAYAAMWAIVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINELLR 368
             +  MWA + + ++  + QKAKEEQE I+K+R   QK L+ KE ++M YL + I+E LR
Sbjct: 314 SGHLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLR 373

Query: 369 RHTIAVASFREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDNYTPK 428
             T ++ +FREAK++V ++ Y IPKGW V  W R +H+DP+IY +P++FDPSRW+ YTPK
Sbjct: 374 VITFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPK 433

Query: 429 PGEFLPFGIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDNCLC 488
            G FLPFG+GS  CPG++L KL I+I LHHF+L Y++ER NP C    LP  +  DNCL 
Sbjct: 434 AGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLA 486

BLAST of PI0010724 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 393.3 bits (1009), Expect = 2.9e-109
Identity = 209/475 (44.00%), Postives = 299/475 (62.95%), Query Frame = 0

Query: 16  GVYFFVRKLNEIWYLVKLGRRVYKSLPPGDLGLPLIGSSFSFYRAFKAGGDPDSFIHHLI 75
           G+ + VRK+N   Y   LG   +  LPPGDLG P IG+  SF RAFK   DPDSF   LI
Sbjct: 21  GLNWLVRKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTLI 80

Query: 76  SRNGRVGMYKSHLYGRPAIIVTDPEICRRIYLDDEKFKPSYPKSVRILEVNGLFSRI--- 135
            R G  G+YK+H++G P+IIVT  + CRR+  DD+ FKP +P S   L     F  I   
Sbjct: 81  KRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFE 140

Query: 136 DHKTAYRIMSSPLNGYEILSKYVSFIDEIMTKGLEDWSTMKEPIELLSEIGSLLFKVIIR 195
           +HK   R+ ++P+NG+E LS Y+ +I+E +   L+ W+ M E  E L+ +  L F++I+ 
Sbjct: 141 EHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMY 200

Query: 196 IFLGNEIPTPTLNMLEALYKHLGPAILSIFPYDLPGLTYHDALKARKEIGNILECVIEEK 255
           IFL +E     ++ LE  Y  L   + ++   ++PG  YH ALKARK +    + ++ E+
Sbjct: 201 IFLSSESEN-VMDALEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQSIVTER 260

Query: 256 RRVLERKETRDQLVKCQMDKLIVAVDENGKKLYDNNAIIDLLLGLLHAGHHTPAYAAMWA 315
           R   +RK+      K  +D L+   DE+GK L D+  IID+LL  L+AGH +  +  MWA
Sbjct: 261 RN--QRKQNILSNKKDMLDNLLNVKDEDGKTL-DDEEIIDVLLMYLNAGHESSGHTIMWA 320

Query: 316 IVQISQNSHVFQKAKEEQEFIMKERPSTQKGLSFKEIKQMKYLMKFINELLRRHTIAVAS 375
            V + ++  V Q+AK EQE I+K RP  QKGLS KE ++M++L + ++E LR  T ++ +
Sbjct: 321 TVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTA 380

Query: 376 FREAKTNVYINDYTIPKGWTVPIWTRAIHMDPQIYSNPQEFDPSRWDN-YTPKPGEFLPF 435
           FREAKT+V +N Y IPKGW V  W R +H+DP+++ +P++FDP+RWDN + PK G FLPF
Sbjct: 381 FREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPF 440

Query: 436 GIGSRFCPGSELTKLYITILLHHFILNYKMERVNPRCDTTHLPAPKLVDNCLCRI 487
           G GS  CPG++L KL I+I LHHF+L Y+++R NP C   +LP  +  DNCL RI
Sbjct: 441 GAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of PI0010724 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 199.9 bits (507), Expect = 4.7e-51
Identity = 134/441 (30.39%), Postives = 224/441 (50.79%), Query Frame = 0

Query: 41  LPPGDLGLPLIGSSFSFYRAFKAGGDPDSFIHHLISRNGRVG-MYKSHLYGRPAIIVTDP 100
           LPPG +GLP IG +   Y       +P+SF     +R  + G ++K+H+ G P ++++ P
Sbjct: 49  LPPGSMGLPYIGETLRLYTE-----NPNSF---FATRQNKYGDIFKTHILGCPCVMISSP 108

Query: 101 EICRRIYLDD-EKFKPSYPKS-VRILEVNGLFSRID--HKTAYRIMSSPLNGYEILSKYV 160
           E  R + +     FKP+YP S  R++    LF      H T  R++ S       L   V
Sbjct: 109 EAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMP-SALRPTV 168

Query: 161 SFIDEIMTKGLEDWSTMKEPIELLSEIGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLG 220
           S I+ ++ + L  W++ K  I  L  +    F V I    G++    T+++++ LY+ L 
Sbjct: 169 SHIELLVLQTLSSWTSQKS-INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLE 228

Query: 221 PAILSIFPYDLPGLTYHDALKARKEIGNILECVIEEKRRVLERKETRDQLVKCQMDKLIV 280
               S+ P DLPG  +H ++KAR E+        EE R+V+E++    +     +  L+ 
Sbjct: 229 RGYNSM-PLDLPGTLFHKSMKARIELS-------EELRKVIEKRRENGREEGGLLGVLLG 288

Query: 281 AVDENGKKLYDNNAIIDLLLGLLHAGHHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMK 340
           A D+    L D+  I D ++G++ A   T A    W +  +  + ++ Q+   EQ  I +
Sbjct: 289 AKDQKRNGLSDSQ-IADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQ 348

Query: 341 ERPSTQKGLSFKEIKQMKYLMKFINELLRRHTIAVASFREAKTNVYINDYTIPKGWTVPI 400
           +     + +S+++ ++M    + I E LR  ++   +FREA  +V  + Y IPKGW V  
Sbjct: 349 KIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLP 408

Query: 401 WTRAIHMDPQIYSNPQEFDPSRWDNYTPKPGEFLPFGIGSRFCPGSELTKLYITILLHHF 460
             R IH   + + +P++FDPSR++   PKP  ++PFG G   CPGSEL KL + ILLHH 
Sbjct: 409 LFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHL 468

Query: 461 ILNYKMERVNPRCDTTHLPAP 477
             +++ E +       + P P
Sbjct: 469 TTSFRWEVIGDEEGIQYGPFP 469

BLAST of PI0010724 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 199.9 bits (507), Expect = 4.7e-51
Identity = 134/441 (30.39%), Postives = 224/441 (50.79%), Query Frame = 0

Query: 41  LPPGDLGLPLIGSSFSFYRAFKAGGDPDSFIHHLISRNGRVG-MYKSHLYGRPAIIVTDP 100
           LPPG +GLP IG +   Y       +P+SF     +R  + G ++K+H+ G P ++++ P
Sbjct: 49  LPPGSMGLPYIGETLRLYTE-----NPNSF---FATRQNKYGDIFKTHILGCPCVMISSP 108

Query: 101 EICRRIYLDD-EKFKPSYPKS-VRILEVNGLFSRID--HKTAYRIMSSPLNGYEILSKYV 160
           E  R + +     FKP+YP S  R++    LF      H T  R++ S       L   V
Sbjct: 109 EAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMP-SALRPTV 168

Query: 161 SFIDEIMTKGLEDWSTMKEPIELLSEIGSLLFKVIIRIFLGNEIPTPTLNMLEALYKHLG 220
           S I+ ++ + L  W++ K  I  L  +    F V I    G++    T+++++ LY+ L 
Sbjct: 169 SHIELLVLQTLSSWTSQKS-INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLE 228

Query: 221 PAILSIFPYDLPGLTYHDALKARKEIGNILECVIEEKRRVLERKETRDQLVKCQMDKLIV 280
               S+ P DLPG  +H ++KAR E+        EE R+V+E++    +     +  L+ 
Sbjct: 229 RGYNSM-PLDLPGTLFHKSMKARIELS-------EELRKVIEKRRENGREEGGLLGVLLG 288

Query: 281 AVDENGKKLYDNNAIIDLLLGLLHAGHHTPAYAAMWAIVQISQNSHVFQKAKEEQEFIMK 340
           A D+    L D+  I D ++G++ A   T A    W +  +  + ++ Q+   EQ  I +
Sbjct: 289 AKDQKRNGLSDSQ-IADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQ 348

Query: 341 ERPSTQKGLSFKEIKQMKYLMKFINELLRRHTIAVASFREAKTNVYINDYTIPKGWTVPI 400
           +     + +S+++ ++M    + I E LR  ++   +FREA  +V  + Y IPKGW V  
Sbjct: 349 KIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLP 408

Query: 401 WTRAIHMDPQIYSNPQEFDPSRWDNYTPKPGEFLPFGIGSRFCPGSELTKLYITILLHHF 460
             R IH   + + +P++FDPSR++   PKP  ++PFG G   CPGSEL KL + ILLHH 
Sbjct: 409 LFRRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHL 468

Query: 461 ILNYKMERVNPRCDTTHLPAP 477
             +++ E +       + P P
Sbjct: 469 TTSFRWEVIGDEEGIQYGPFP 469