PI0010481 (gene) Melon (PI 482460) v1

BLAST of PI0010481 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 1.0e-56
Identity = 162/530 (30.57%), Postives = 274/530 (51.70%), Query Frame = 0

Query: 15  KWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLAGLSS 74
           KW  LV+AIW+Q+ +G N+ FS YS+ LKS++ +SQ++LN L+VA DLGKAFG  +G++ 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 75  DRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLC--MGGNSTTWMNTGVL 134
                S++L   +  G +GYG QWLV++  I  LPY    +FLC  + G S  W NT   
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVIT-NIITLPY--SLVFLCCLLAGLSICWFNTACF 162

Query: 135 VTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFAVCLTAI 194
           + C+R+F  NR     +   +  +S A+++   +A+   + + +L L SLVP  V   A+
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAAL 222

Query: 195 IFLREIPF-----SADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLSRIFSIVL 254
             +   P        D    D   F +LN ++V  +  LL+       ++ L+ I ++VL
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLL-SSSSTSSARLNFIGAVVL 282

Query: 255 LSL-LASPLVI-------PLHSFLKNRGGSGEV------------AEALLAGESAADDGV 314
           L   L +PL++       P+ +   N   SG V            + +   G        
Sbjct: 283 LVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAK 342

Query: 315 EGKPV-IGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFIS 374
           EG  V +G++H+    +  +EFW+ ++++ CG   GL   NN+GQI  +LG +  ++ ++
Sbjct: 343 EGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTTL-VT 402

Query: 375 LISIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMALGYIILAIAI--PGSLYIGS 434
           + S + FFGR+LS +A +   K+  + R  W A + +   + + +LA++     +L   +
Sbjct: 403 IYSSFSFFGRLLS-AAPDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTAT 462

Query: 435 IVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEA-- 494
            ++G+  G   A  V + S++FG  + G+ +N+LI N+P+GS L+ G +A  +Y+  A  
Sbjct: 463 ALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLY-GYIAASIYEANASP 522

Query: 495 --TTTKRGGNTCVGAHCYRLVFVVMACSCVIGFAMDLFLAFRTKRLYSKM 511
             T        C+G  CY   FV   C  ++G    L L  RTK +Y ++
Sbjct: 523 DITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRL 565

BLAST of PI0010481 vs. ExPASy Swiss-Prot
Match: Q5AXV1 (Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mch1 PE=3 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 1.2e-06
Identity = 116/544 (21.32%), Postives = 203/544 (37.32%), Query Frame = 0

Query: 5   RRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGK 64
           RR      +  W G+++ +   SI+     FS Y   L + +N +QL++N +S+A  +  
Sbjct: 75  RREVVRVGSFVW-GVITCLGAGSIT----AFSLYGPLLLTRLNYTQLRVNEVSIAAGISM 134

Query: 65  AFGL-LAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKP------LPYWQMCIFL 124
              + LAG   DR S S L L   I   +GY     V      P       P+W M +  
Sbjct: 135 YLPVSLAGYLCDRYSPSPLTLFAGIAFGLGYSLAAFVYKSGPPPDAGGNGWPFWVMVVAF 194

Query: 125 CMGGNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIF------------TDLC 184
              G +T  M    + TC +NF   RG   GI+   +A+  A F              LC
Sbjct: 195 IAIGVATCSMYLAAVTTCAKNF--GRGKHKGII---LAVPIAAFGLSGMWQSQVGTYFLC 254

Query: 185 SALFSDNPS-----SFLALLSLVPFAVCLTAIIFLREIPFSADDETEDFKYFWVLNAVSV 244
             L   N        +   L+++   + +     LR +    DDE E +    +  AV  
Sbjct: 255 ERLKDSNCGDVDVYKYFLFLAILLLTIGVIGTFALRIV----DDEEEKY----IDEAVEE 314

Query: 245 AVAVTLLVFDLIPNPNSFLSRIFSIVLLSLLASPLVIPLHSFLKNRGGSGEV-AEALLAG 304
                LL       P S +   +                 +F  +   +G V  +++   
Sbjct: 315 LERSGLLAESEFFRPRSEVQAAYG----------------TFSDDHEDNGSVDDQSVTIS 374

Query: 305 ESAADDGVEGKPVIGEDHTIVEAMKTVE---------FWILFVSFLCGVGTGLAVMNNMG 364
           E   +     K    E+      +   E          W L + F    G G A +NN+G
Sbjct: 375 EELREAARREKEREEEERRKKNWLLNYETRIFLADHTMWWLALGFFLVTGPGEAYLNNLG 434

Query: 365 QIGLALGYDDVSI-----------FISLISIWGFFGRILSGSASE--------HFI---- 424
            I   L  D  +I            +++I++     R+L+GS S+        HF     
Sbjct: 435 TIVQTLNLDTTAIVDSHPAGLPSTHVTIIALTSTIARLLTGSLSDLFAPTARRHFTVDQE 494

Query: 425 ---------KKAGMPRPLWNAASQILMALGYIILAIAIPGS----LYIGSIVVGICYGVR 479
                    ++  + R  +   S +L++LG+++LA  +P       ++ + +VG+ YG  
Sbjct: 495 TAGPDPFTKQRPALSRLAFLIPSALLLSLGFLLLASPLPTHHPELSHLTTALVGLGYGSI 554

BLAST of PI0010481 vs. ExPASy Swiss-Prot
Match: P37662 (Uncharacterized MFS-type transporter YhjX OS=Escherichia coli (strain K12) OX=83333 GN=yhjX PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-04
Identity = 41/158 (25.95%), Postives = 74/158 (46.84%), Query Frame = 0

Query: 292 VIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDV---SIFISLI 351
           V+ +D+T+ E+M+  ++W+L V FL    +GL V+     I  +L + DV   +  +++I
Sbjct: 200 VVEKDYTLAESMRKPQYWMLAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVI 259

Query: 352 SIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIVVG 411
           SI    GR++ G  S+    K    R +       L+ +  ++ A     + +     V 
Sbjct: 260 SIANLSGRLVLGILSD----KIARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVA 319

Query: 412 ICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGS 447
             +G  + +   + SE FGL N    Y V+ L   +GS
Sbjct: 320 FNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIGS 353

BLAST of PI0010481 vs. ExPASy Swiss-Prot
Match: Q4WVT3 (Probable transporter mch1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mch1 PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 4.4e-04
Identity = 117/544 (21.51%), Postives = 212/544 (38.97%), Query Frame = 0

Query: 17  LGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGL-LAGLSSD 76
           +G++S +   SI+     FS Y     + ++ SQLQ+N +S+A ++     + L G   D
Sbjct: 86  VGVISCLGAGSIT----AFSLYGPLFLTRLHYSQLQVNAVSIAAEISMYLPVPLFGYLCD 145

Query: 77  RLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPY--------WQMCIFLCMGGNSTTWM 136
           R + S L L+  +  + G G      + +  PLP         W M +     G +T+ M
Sbjct: 146 RYTPSPLALLSGL--VFGGGYLLAAFAYRSGPLPEAGGEGWPPWVMVVAFVAIGTATSCM 205

Query: 137 NTGVLVTCLRNFRR--NRGTVSGILKGYIALSTAIFTDLCSALFSDNPSS--------FL 196
               + TC +NF R  ++G +  +      LS    + + + L  +            F 
Sbjct: 206 YLAAVTTCAKNFGRGKHKGIMLAVPIAGFGLSGMWQSQVATYLLCERREDGSRGDVDVFR 265

Query: 197 ALLSLVPFAVCLTAI--IFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNP 256
             L L  F  CL  I    LR +     DE ED    ++  AV       LL       P
Sbjct: 266 YFLFLALFLFCLGVIGTFGLRIV-----DEEED---QYIDEAVEELERSGLLEESEFFRP 325

Query: 257 NSFLSRIFSIVLLSLLASPLVIPLHSFLKNRGGSGEVAEALLAGESAADDGVEGKPVIGE 316
            S +   +     +         L   L       E   A L  E   ++  +   ++  
Sbjct: 326 RSEVQAAYGTFSDAADGDAPGPELSLTLSEE----EREAARLEKEREEEERRKKNWLLNF 385

Query: 317 DHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYD-----------DVSIF 376
           +  +    +T+  W L V F    G G A +NN+G I   L  +             S  
Sbjct: 386 ETRLFLQDQTM--WWLAVGFFLVTGPGEAYINNLGTIIQTLTPELHPPNAPSPAGLPSTH 445

Query: 377 ISLISIWGFFGRILSGSASEHF------------------------IKKAGMPRPLWNAA 436
           +++I++     R+L+GS S+ F                         K+  + R  +   
Sbjct: 446 VTIIALTSTIARLLTGSLSDFFAPPATHLFPANIESGRRSSQSGPTAKRPTLSRLAFLLP 505

Query: 437 SQILMALGYIILAIAI----PGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYN 496
           S +L++LGY++L+  +    PG  ++ + ++G+ YG   ++   + S V+G++N+G  + 
Sbjct: 506 SALLLSLGYLLLSSPLPLQHPGLSHVTTALIGLGYGSAFSLVPIIISVVWGVENFGTNWG 565

BLAST of PI0010481 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 4.4e-04
Identity = 60/254 (23.62%), Postives = 98/254 (38.58%), Query Frame = 0

Query: 262 FLKNRGGSGEVAEALLAGESAADDGVEGKPVIGEDHTIVEAMKTVEFWILFVSFLCGVGT 321
           F   R      +E     E++ D   +G     E  T+ E       W+  + F+   G 
Sbjct: 230 FFSCRTAESMASELRKKAEASTDCNCDGPG--HEGATLKEFFTDKTAWLFLLCFVFIGGP 289

Query: 322 GLAVMNNMGQIGLALGYDDV-----SIFISLISIWGFFGRILSGSASEHFIKKAGMPRPL 381
                NNMG I   +  ++      S  +SL + +    R++ G +SE        P  L
Sbjct: 290 FEMFQNNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSSEAMESHVSRPVLL 349

Query: 382 WNAASQILMALGYIILAIAIPGSLY----------IGSIVVGICYGVRLAITVPMASEVF 441
                  ++AL    + + +P  ++          + +IV G  YG    +   + ++V+
Sbjct: 350 ------SVIALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFTLVPTIVTKVW 409

Query: 442 GLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATT-TKRGGNTCVGAHCYRLVFVVM 500
           G+ N G I+   IL L +GS L  GLL   +YD  +          C G HCY L FV+ 
Sbjct: 410 GIANLGTIWGSFILALAVGS-LGYGLLFAKVYDAASEVGVGSMSQVCSGVHCYGLTFVIT 469

BLAST of PI0010481 vs. ExPASy TrEMBL
Match: A0A1S3BI29 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103490296 PE=4 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.2e-275
Identity = 496/515 (96.31%), Postives = 505/515 (98.06%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60
           MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSL+NLSQLQLNNLSVAK
Sbjct: 25  MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 84

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAGLSSDRLSTSLLL IGSIEGLIGYGAQWLVVS+KIKPLPYWQMCIFLCMG
Sbjct: 85  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 144

Query: 121 GNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFRR+RGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL
Sbjct: 145 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 204

Query: 181 SLVPFAVCLTAIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLS 240
           SLVPF VCLTAI+FLREIP SADDETE+FKYFWVLNAVSVAVAVTLLVFD IPNPNSFLS
Sbjct: 205 SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 264

Query: 241 RIFSIVLLSLLASPLVIPLHSFLKNRGGSGEVAEALLAGESAADDGVEGKPVIGEDHTIV 300
           RIFSI+LLSLLASPLVIPLHSFLKNRGGSGEV EALLAGESAADD VEGKP IGEDHTIV
Sbjct: 265 RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV 324

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRILSG 360
           EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGY+DVSIFISL+SIWGFFGRILSG
Sbjct: 325 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYEDVSIFISLMSIWGFFGRILSG 384

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           SASEHFIKKAGMPRPLWNAASQILMALGYI+LAIAIPGSLYIGSIVVGICYGVRLAITVP
Sbjct: 385 SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP 444

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKRGGNTCVGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTK GGNTC GAHCYRL
Sbjct: 445 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL 504

Query: 481 VFVVMACSCVIGFAMDLFLAFRTKRLYSKMRALKR 516
           VFVVMACSC IGFAMDLFLAFRTKRLYSKMRALKR
Sbjct: 505 VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR 539

BLAST of PI0010481 vs. ExPASy TrEMBL
Match: A0A6J1JP36 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486233 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.6e-233
Identity = 420/512 (82.03%), Postives = 464/512 (90.62%), Query Frame = 0

Query: 1   MNIFR-RSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVA 60
           M+IF    PPSSSA+KWL LVSA+WVQSI+GNNYTFSNYSAALKSLMNLSQLQLNNLSVA
Sbjct: 1   MSIFHPPPPPSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVA 60

Query: 61  KDLGKAFGLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCM 120
           KDLGKAFGLL+GL+SDRL TSLLLLIGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+CM
Sbjct: 61  KDLGKAFGLLSGLASDRLPTSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCM 120

Query: 121 GGNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLAL 180
           GGNS+TWMNT VLVTCLRNFRRN G+VSG+LKGY+ALSTAIFTDLC ALFS++PSSFLAL
Sbjct: 121 GGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLAL 180

Query: 181 LSLVPFAVCLTAIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFL 240
           L++VPFAVCL AI+FLRE PF+ D+ETED +YFWVLNA++VAVAV+LL FDLIPNP S +
Sbjct: 181 LAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDLIPNPTSSV 240

Query: 241 SRIFSIVLLSLLASPLVIPLHSFLKNRGGSGEVAEALLAGESAADDGVEGKPVIGEDHTI 300
           S IFSIVLL LL SPLVIP+HSFLKNRG SG  AEALLA E  A+    G PVIGEDHTI
Sbjct: 241 SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHTI 300

Query: 301 VEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRILS 360
           VEAMKTVEFW+LFVSFL GVGTGLAVMNNMGQIGLALGYDDVS FISL+SIWGFFGRILS
Sbjct: 301 VEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILS 360

Query: 361 GSASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAITV 420
           GSASE+FIKKAGM RPLWNAASQILMA+GYI+LAI +P SLYIGSI+VGICYGVRLAI+V
Sbjct: 361 GSASEYFIKKAGMARPLWNAASQILMAIGYILLAIGMPSSLYIGSIIVGICYGVRLAISV 420

Query: 421 PMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKRGGNTCVGAHCYR 480
           P+ASE+FGLK+YGMIYNVLILNLPLGSFLFSGLLAGILYDMEAT  + GGNTC+GAHCYR
Sbjct: 421 PIASEIFGLKDYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATKIEEGGNTCMGAHCYR 480

Query: 481 LVFVVMACSCVIGFAMDLFLAFRTKRLYSKMR 512
           LVFVVMACSCV+G  +DL L  +TKRLYSK R
Sbjct: 481 LVFVVMACSCVVGLGVDLLLFIKTKRLYSKTR 512

BLAST of PI0010481 vs. ExPASy TrEMBL
Match: A0A6J1ENR3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435199 PE=4 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.4e-231
Identity = 417/513 (81.29%), Postives = 461/513 (89.86%), Query Frame = 0

Query: 1   MNIFRRSPP--SSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60
           M+IF   PP  SSSA+KWL LVSA+WVQSI+GNNYTFSNYSAALKSLMNLSQLQLNNLSV
Sbjct: 1   MSIFHPPPPPSSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSV 60

Query: 61  AKDLGKAFGLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLC 120
           AKDLGKAFGLL+GL+SDRL  SLLLLIGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+C
Sbjct: 61  AKDLGKAFGLLSGLASDRLPPSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMC 120

Query: 121 MGGNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLA 180
           MGGNS+TWMNT VLVTCLRNFRRN G+VSG+LKGY+ALSTAIFTDLC ALFS++PSSFLA
Sbjct: 121 MGGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLA 180

Query: 181 LLSLVPFAVCLTAIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSF 240
           LL++VPFAVCL AI+FLRE PF+ D+ETED +YFWVLNA++VAVAV+LL FD IPNP S 
Sbjct: 181 LLAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDSIPNPTSS 240

Query: 241 LSRIFSIVLLSLLASPLVIPLHSFLKNRGGSGEVAEALLAGESAADDGVEGKPVIGEDHT 300
           +S IFSIVLL LL SPLVIP+HSFLKNRG SG  AEALLA E  A+    G PVIGEDHT
Sbjct: 241 VSIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHT 300

Query: 301 IVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRIL 360
           IVEAMKTVEFW+LFVSFL GVGTGLAVMNNMGQIGLALGYDDVS FISL+SIWGFFGRIL
Sbjct: 301 IVEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRIL 360

Query: 361 SGSASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAIT 420
           SGSASE+FIKKAGM RPLWNAASQILMA+G I+LAI +P SLYIGSI+VGICYGVRLAI+
Sbjct: 361 SGSASEYFIKKAGMARPLWNAASQILMAIGCILLAIGMPSSLYIGSIIVGICYGVRLAIS 420

Query: 421 VPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKRGGNTCVGAHCY 480
           VP+ASE+FGLK+YGMIYNVLI NLPLGSFLFSGLLAGILYDMEAT T+ GGNTC+G HCY
Sbjct: 421 VPIASEIFGLKDYGMIYNVLIFNLPLGSFLFSGLLAGILYDMEATKTEEGGNTCMGEHCY 480

Query: 481 RLVFVVMACSCVIGFAMDLFLAFRTKRLYSKMR 512
           RLVFVVMACSCV+G  +DL L  RTKRLYSK+R
Sbjct: 481 RLVFVVMACSCVVGLGVDLLLFIRTKRLYSKIR 513

BLAST of PI0010481 vs. ExPASy TrEMBL
Match: I1JSE9 (Nodulin-like domain-containing protein OS=Glycine max OX=3847 GN=100813607 PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 1.1e-207
Identity = 367/528 (69.51%), Postives = 443/528 (83.90%), Query Frame = 0

Query: 11  SSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLA 70
           S+  KWLGLV+A+W+Q+ISGNNYTFSNYS ALKSLM+L+Q++LNNLSVAKD+GKAFGLLA
Sbjct: 6   STGSKWLGLVAAVWIQAISGNNYTFSNYSDALKSLMSLTQIELNNLSVAKDVGKAFGLLA 65

Query: 71  GLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMGGNSTTWMNTG 130
           GL+SD+  T  +LLIGS++GLIGYG QWLVVSQ+I+PLPYWQMC+FLC+GGNSTTWMNT 
Sbjct: 66  GLASDKFPTWAILLIGSLQGLIGYGVQWLVVSQRIQPLPYWQMCVFLCIGGNSTTWMNTA 125

Query: 131 VLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFAVCLT 190
           VLVTC+RNFRRNRG VSGILKG++ LSTAIFTDLCSALF+D+P+SFL +L+L+PFAVCL+
Sbjct: 126 VLVTCIRNFRRNRGPVSGILKGFVGLSTAIFTDLCSALFADDPASFLLMLALIPFAVCLS 185

Query: 191 AIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLSRIFSIVLLSL 250
            + FLREIP +A ++ E+  YF V NAV+V VAV LL F  +PNP++ +SR F++VLL L
Sbjct: 186 GMFFLREIPPAATNDQEESTYFAVFNAVAVVVAVYLLAFGFVPNPSALVSRAFAVVLLLL 245

Query: 251 LASPLVIPLHSFLKNR------------------------GGSGEVAEALLAGESAADDG 310
           L +P+ IP+HS+LK R                        G   EV    +  E AA +G
Sbjct: 246 LVAPMGIPVHSYLKARRQDERFKPNLEERVDEPLIRGKEKGSESEVERGNVLAEEAAAEG 305

Query: 311 VEGKPVIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFIS 370
           + G P +GE+HTI EA+KTV+FWILFVSFLCGVGTGLAVMNNMGQIGLALGY DVS+F+S
Sbjct: 306 MSG-PAVGEEHTIWEALKTVDFWILFVSFLCGVGTGLAVMNNMGQIGLALGYSDVSLFVS 365

Query: 371 LISIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIV 430
           L SIWGFFGRI+SGS SEHFIKKA  PRPLWNAASQILMA+GYI+LA+A+PGSLYIGS+V
Sbjct: 366 LTSIWGFFGRIVSGSVSEHFIKKAATPRPLWNAASQILMAVGYILLAMAMPGSLYIGSVV 425

Query: 431 VGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTK 490
           VGICYGVRLAITVP ASE+FGLK YG+IYN+LILNLPLGSFLFSGLLAGILYDMEATTT 
Sbjct: 426 VGICYGVRLAITVPTASELFGLKYYGLIYNILILNLPLGSFLFSGLLAGILYDMEATTTV 485

Query: 491 RGGNTCVGAHCYRLVFVVMACSCVIGFAMDLFLAFRTKRLYSKMRALK 515
            GGNTC+GAHCYRLVF++MA +CV+GF +D+ L+FRTK++Y+K+ + K
Sbjct: 486 GGGNTCIGAHCYRLVFIIMAVACVVGFFLDILLSFRTKKVYNKISSSK 532

BLAST of PI0010481 vs. ExPASy TrEMBL
Match: A0A445KUP2 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Glycine soja OX=3848 GN=D0Y65_008366 PE=4 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 1.1e-207
Identity = 367/528 (69.51%), Postives = 443/528 (83.90%), Query Frame = 0

Query: 11  SSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLA 70
           S+  KWLGLV+A+W+Q+ISGNNYTFSNYS ALKSLM+L+Q++LNNLSVAKD+GKAFGLLA
Sbjct: 6   STGSKWLGLVAAVWIQAISGNNYTFSNYSDALKSLMSLTQIELNNLSVAKDVGKAFGLLA 65

Query: 71  GLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMGGNSTTWMNTG 130
           GL+SD+  T  +LLIGS++GLIGYG QWLVVSQ+I+PLPYWQMC+FLC+GGNSTTWMNT 
Sbjct: 66  GLASDKFPTWAILLIGSLQGLIGYGVQWLVVSQRIQPLPYWQMCVFLCIGGNSTTWMNTA 125

Query: 131 VLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFAVCLT 190
           VLVTC+RNFRRNRG VSGILKG++ LSTAIFTDLCSALF+D+P+SFL +L+L+PFAVCL+
Sbjct: 126 VLVTCIRNFRRNRGPVSGILKGFVGLSTAIFTDLCSALFADDPASFLLMLALIPFAVCLS 185

Query: 191 AIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLSRIFSIVLLSL 250
            + FLREIP +A ++ E+  YF V NAV+V VAV LL F  +PNP++ +SR F++VLL L
Sbjct: 186 GMFFLREIPPAATNDQEESTYFAVFNAVAVVVAVYLLAFGFVPNPSALVSRAFAVVLLLL 245

Query: 251 LASPLVIPLHSFLKNR------------------------GGSGEVAEALLAGESAADDG 310
           L +P+ IP+HS+LK R                        G   EV    +  E AA +G
Sbjct: 246 LVAPMGIPVHSYLKARRQDERFKPNLEERVDEPLIRGKEKGSESEVERGNVLAEEAAAEG 305

Query: 311 VEGKPVIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFIS 370
           + G P +GE+HTI EA+KTV+FWILFVSFLCGVGTGLAVMNNMGQIGLALGY DVS+F+S
Sbjct: 306 MSG-PAVGEEHTIWEALKTVDFWILFVSFLCGVGTGLAVMNNMGQIGLALGYSDVSLFVS 365

Query: 371 LISIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIV 430
           L SIWGFFGRI+SGS SEHFIKKA  PRPLWNAASQILMA+GYI+LA+A+PGSLYIGS+V
Sbjct: 366 LTSIWGFFGRIVSGSVSEHFIKKAATPRPLWNAASQILMAVGYILLAMAMPGSLYIGSVV 425

Query: 431 VGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTK 490
           VGICYGVRLAITVP ASE+FGLK YG+IYN+LILNLPLGSFLFSGLLAGILYDMEATTT 
Sbjct: 426 VGICYGVRLAITVPTASELFGLKYYGLIYNILILNLPLGSFLFSGLLAGILYDMEATTTV 485

Query: 491 RGGNTCVGAHCYRLVFVVMACSCVIGFAMDLFLAFRTKRLYSKMRALK 515
            GGNTC+GAHCYRLVF++MA +CV+GF +D+ L+FRTK++Y+K+ + K
Sbjct: 486 GGGNTCIGAHCYRLVFIIMAVACVVGFFLDILLSFRTKKVYNKISSSK 532

BLAST of PI0010481 vs. NCBI nr
Match: XP_008447967.2 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo])

HSP 1 Score: 958.4 bits (2476), Expect = 2.6e-275
Identity = 496/515 (96.31%), Postives = 505/515 (98.06%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60
           MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSL+NLSQLQLNNLSVAK
Sbjct: 25  MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLLNLSQLQLNNLSVAK 84

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAGLSSDRLSTSLLL IGSIEGLIGYGAQWLVVS+KIKPLPYWQMCIFLCMG
Sbjct: 85  DLGKAFGLLAGLSSDRLSTSLLLFIGSIEGLIGYGAQWLVVSRKIKPLPYWQMCIFLCMG 144

Query: 121 GNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFRR+RGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL
Sbjct: 145 GNSTTWMNTGVLVTCLRNFRRDRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 204

Query: 181 SLVPFAVCLTAIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLS 240
           SLVPF VCLTAI+FLREIP SADDETE+FKYFWVLNAVSVAVAVTLLVFD IPNPNSFLS
Sbjct: 205 SLVPFIVCLTAILFLREIPCSADDETENFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 264

Query: 241 RIFSIVLLSLLASPLVIPLHSFLKNRGGSGEVAEALLAGESAADDGVEGKPVIGEDHTIV 300
           RIFSI+LLSLLASPLVIPLHSFLKNRGGSGEV EALLAGESAADD VEGKP IGEDHTIV
Sbjct: 265 RIFSIILLSLLASPLVIPLHSFLKNRGGSGEVGEALLAGESAADDAVEGKPAIGEDHTIV 324

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRILSG 360
           EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGY+DVSIFISL+SIWGFFGRILSG
Sbjct: 325 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYEDVSIFISLMSIWGFFGRILSG 384

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           SASEHFIKKAGMPRPLWNAASQILMALGYI+LAIAIPGSLYIGSIVVGICYGVRLAITVP
Sbjct: 385 SASEHFIKKAGMPRPLWNAASQILMALGYIVLAIAIPGSLYIGSIVVGICYGVRLAITVP 444

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKRGGNTCVGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTK GGNTC GAHCYRL
Sbjct: 445 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCTGAHCYRL 504

Query: 481 VFVVMACSCVIGFAMDLFLAFRTKRLYSKMRALKR 516
           VFVVMACSC IGFAMDLFLAFRTKRLYSKMRALKR
Sbjct: 505 VFVVMACSCGIGFAMDLFLAFRTKRLYSKMRALKR 539

BLAST of PI0010481 vs. NCBI nr
Match: XP_011658596.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis sativus] >KAE8645667.1 hypothetical protein Csa_020437 [Cucumis sativus])

HSP 1 Score: 949.1 bits (2452), Expect = 1.6e-272
Identity = 490/515 (95.15%), Postives = 501/515 (97.28%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60
           MN FRRSPPS SALKWLGLV+AIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK
Sbjct: 1   MNSFRRSPPSPSALKWLGLVTAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAGL+SDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG
Sbjct: 61  DLGKAFGLLAGLASDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG 120

Query: 121 GNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFR+NRGTVSGILKGYIALSTAIFTD CSALFSDNPSSFLALL
Sbjct: 121 GNSTTWMNTGVLVTCLRNFRKNRGTVSGILKGYIALSTAIFTDFCSALFSDNPSSFLALL 180

Query: 181 SLVPFAVCLTAIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLS 240
           SLVPFAVCL AI+FLRE+P SADDETE+FKYFWVLNAVSVAVAVTLLVFD IPNPNSFLS
Sbjct: 181 SLVPFAVCLAAILFLREVPRSADDETEEFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 240

Query: 241 RIFSIVLLSLLASPLVIPLHSFLKNRGGSGEVAEALLAGESAADDGVEGKPVIGEDHTIV 300
           RIF IVLLSLL SPLVIPLHSFLKN+GGSGEVAEALLAGES AD+ VEGKPVIGEDHTIV
Sbjct: 241 RIFCIVLLSLLVSPLVIPLHSFLKNKGGSGEVAEALLAGESGADNAVEGKPVIGEDHTIV 300

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRILSG 360
           EAMKT EFWI+FVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISL+SIWGFFGRILSG
Sbjct: 301 EAMKTFEFWIMFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           SASEHFIKKAGMPRPLWNAASQILMALGYI+LAIAIPGSLYIGSIVVGICYGVRLAITVP
Sbjct: 361 SASEHFIKKAGMPRPLWNAASQILMALGYILLAIAIPGSLYIGSIVVGICYGVRLAITVP 420

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKRGGNTCVGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTK GGNTCVGAHCYRL
Sbjct: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCVGAHCYRL 480

Query: 481 VFVVMACSCVIGFAMDLFLAFRTKRLYSKMRALKR 516
           VFVVMACSC IGFAMD FLAFRTKRLYSKMRALKR
Sbjct: 481 VFVVMACSCAIGFAMDSFLAFRTKRLYSKMRALKR 515

BLAST of PI0010481 vs. NCBI nr
Match: XP_038887463.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 903.3 bits (2333), Expect = 9.8e-259
Identity = 465/513 (90.64%), Postives = 486/513 (94.74%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60
           M+IF R PPSSSALKWLGLV+A+WVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK
Sbjct: 1   MSIFHRPPPSSSALKWLGLVTAVWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAG++SDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKI PLPYWQMCIFLCMG
Sbjct: 61  DLGKAFGLLAGIASDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKINPLPYWQMCIFLCMG 120

Query: 121 GNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTD+CSALFSDNPSSFLALL
Sbjct: 121 GNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDICSALFSDNPSSFLALL 180

Query: 181 SLVPFAVCLTAIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLS 240
           SLVPFAVCLTAI+FLREIP SAD+ETED +YFW+LNAVSV VAVTLL FD IPNP S +S
Sbjct: 181 SLVPFAVCLTAILFLREIPCSADNETEDSRYFWILNAVSVIVAVTLLAFDSIPNPTSSIS 240

Query: 241 RIFSIVLLSLLASPLVIPLHSFLKNRGGSGEVAEALLAGESAADDGVEGKPVIGEDHTIV 300
           RIFSIVLL  LASPL IPLHSFLKNRG SGE+AEALLA ES A+  VEG+PVIGEDHTIV
Sbjct: 241 RIFSIVLLIFLASPLAIPLHSFLKNRGRSGEIAEALLADESVAEGVVEGQPVIGEDHTIV 300

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRILSG 360
           EAMKTVEFWILF SFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISL+SIWGFFGRILSG
Sbjct: 301 EAMKTVEFWILFGSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           S SEHFI+K GMPRPLWNAASQ+LMALGYI+LAIA+PGSLYIGSI+VGICYGVRLAITVP
Sbjct: 361 SVSEHFIRKDGMPRPLWNAASQVLMALGYILLAIAMPGSLYIGSIIVGICYGVRLAITVP 420

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKRGGNTCVGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEAT  K GGN+CVG HCYRL
Sbjct: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATRRKGGGNSCVGGHCYRL 480

Query: 481 VFVVMACSCVIGFAMDLFLAFRTKRLYSKMRAL 514
           VFVVMACSCVIGFAMDLFLA RTKRLYSKMR L
Sbjct: 481 VFVVMACSCVIGFAMDLFLASRTKRLYSKMRVL 513

BLAST of PI0010481 vs. NCBI nr
Match: XP_031744364.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X2 [Cucumis sativus])

HSP 1 Score: 838.6 bits (2165), Expect = 3.0e-239
Identity = 443/515 (86.02%), Postives = 453/515 (87.96%), Query Frame = 0

Query: 1   MNIFRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60
           MN FRRSPPS SALKWLGLV+AIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK
Sbjct: 1   MNSFRRSPPSPSALKWLGLVTAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAK 60

Query: 61  DLGKAFGLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG 120
           DLGKAFGLLAGL+SDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG
Sbjct: 61  DLGKAFGLLAGLASDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMG 120

Query: 121 GNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALL 180
           GNSTTWMNTGVLVTCLRNFR+NRGTVS                                 
Sbjct: 121 GNSTTWMNTGVLVTCLRNFRKNRGTVS--------------------------------- 180

Query: 181 SLVPFAVCLTAIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLS 240
                            +P SADDETE+FKYFWVLNAVSVAVAVTLLVFD IPNPNSFLS
Sbjct: 181 -----------------VPRSADDETEEFKYFWVLNAVSVAVAVTLLVFDSIPNPNSFLS 240

Query: 241 RIFSIVLLSLLASPLVIPLHSFLKNRGGSGEVAEALLAGESAADDGVEGKPVIGEDHTIV 300
           RIF IVLLSLL SPLVIPLHSFLKN+GGSGEVAEALLAGES AD+ VEGKPVIGEDHTIV
Sbjct: 241 RIFCIVLLSLLVSPLVIPLHSFLKNKGGSGEVAEALLAGESGADNAVEGKPVIGEDHTIV 300

Query: 301 EAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRILSG 360
           EAMKT EFWI+FVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISL+SIWGFFGRILSG
Sbjct: 301 EAMKTFEFWIMFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLMSIWGFFGRILSG 360

Query: 361 SASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAITVP 420
           SASEHFIKKAGMPRPLWNAASQILMALGYI+LAIAIPGSLYIGSIVVGICYGVRLAITVP
Sbjct: 361 SASEHFIKKAGMPRPLWNAASQILMALGYILLAIAIPGSLYIGSIVVGICYGVRLAITVP 420

Query: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKRGGNTCVGAHCYRL 480
           MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTK GGNTCVGAHCYRL
Sbjct: 421 MASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKTGGNTCVGAHCYRL 465

Query: 481 VFVVMACSCVIGFAMDLFLAFRTKRLYSKMRALKR 516
           VFVVMACSC IGFAMD FLAFRTKRLYSKMRALKR
Sbjct: 481 VFVVMACSCAIGFAMDSFLAFRTKRLYSKMRALKR 465

BLAST of PI0010481 vs. NCBI nr
Match: XP_022989043.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 817.8 bits (2111), Expect = 5.4e-233
Identity = 420/512 (82.03%), Postives = 464/512 (90.62%), Query Frame = 0

Query: 1   MNIFR-RSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVA 60
           M+IF    PPSSSA+KWL LVSA+WVQSI+GNNYTFSNYSAALKSLMNLSQLQLNNLSVA
Sbjct: 1   MSIFHPPPPPSSSAVKWLSLVSAVWVQSIAGNNYTFSNYSAALKSLMNLSQLQLNNLSVA 60

Query: 61  KDLGKAFGLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCM 120
           KDLGKAFGLL+GL+SDRL TSLLLLIGS+EGLIGYG QWLVVS+ I PLPYWQ+ IF+CM
Sbjct: 61  KDLGKAFGLLSGLASDRLPTSLLLLIGSLEGLIGYGGQWLVVSKTINPLPYWQISIFMCM 120

Query: 121 GGNSTTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLAL 180
           GGNS+TWMNT VLVTCLRNFRRN G+VSG+LKGY+ALSTAIFTDLC ALFS++PSSFLAL
Sbjct: 121 GGNSSTWMNTAVLVTCLRNFRRNIGSVSGVLKGYVALSTAIFTDLCFALFSNDPSSFLAL 180

Query: 181 LSLVPFAVCLTAIIFLREIPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFL 240
           L++VPFAVCL AI+FLRE PF+ D+ETED +YFWVLNA++VAVAV+LL FDLIPNP S +
Sbjct: 181 LAVVPFAVCLIAILFLRETPFAVDNETEDSQYFWVLNAIAVAVAVSLLAFDLIPNPTSSV 240

Query: 241 SRIFSIVLLSLLASPLVIPLHSFLKNRGGSGEVAEALLAGESAADDGVEGKPVIGEDHTI 300
           S IFSIVLL LL SPLVIP+HSFLKNRG SG  AEALLA E  A+    G PVIGEDHTI
Sbjct: 241 SIIFSIVLLILLVSPLVIPIHSFLKNRGRSGAAAEALLAAEEVAEVEGNGNPVIGEDHTI 300

Query: 301 VEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRILS 360
           VEAMKTVEFW+LFVSFL GVGTGLAVMNNMGQIGLALGYDDVS FISL+SIWGFFGRILS
Sbjct: 301 VEAMKTVEFWLLFVSFLFGVGTGLAVMNNMGQIGLALGYDDVSSFISLMSIWGFFGRILS 360

Query: 361 GSASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAITV 420
           GSASE+FIKKAGM RPLWNAASQILMA+GYI+LAI +P SLYIGSI+VGICYGVRLAI+V
Sbjct: 361 GSASEYFIKKAGMARPLWNAASQILMAIGYILLAIGMPSSLYIGSIIVGICYGVRLAISV 420

Query: 421 PMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATTTKRGGNTCVGAHCYR 480
           P+ASE+FGLK+YGMIYNVLILNLPLGSFLFSGLLAGILYDMEAT  + GGNTC+GAHCYR
Sbjct: 421 PIASEIFGLKDYGMIYNVLILNLPLGSFLFSGLLAGILYDMEATKIEEGGNTCMGAHCYR 480

Query: 481 LVFVVMACSCVIGFAMDLFLAFRTKRLYSKMR 512
           LVFVVMACSCV+G  +DL L  +TKRLYSK R
Sbjct: 481 LVFVVMACSCVVGLGVDLLLFIKTKRLYSKTR 512

BLAST of PI0010481 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 718.4 bits (1853), Expect = 4.1e-207
Identity = 371/535 (69.35%), Postives = 439/535 (82.06%), Query Frame = 0

Query: 4   FRRSPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLG 63
           F+RS  SSSALKWLG V+A+WVQSISGNNYTFSNYS ALKSLMNL+QL+LNNLSVAKD+G
Sbjct: 3   FQRSISSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKDVG 62

Query: 64  KAFGLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMGGNS 123
           KAFG+LAGL+SDRL T ++LLIG  EGL+GYG QWLVVS+ I+P+PYWQMCIFLCMGGNS
Sbjct: 63  KAFGILAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGGNS 122

Query: 124 TTWMNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLV 183
           TTWMNT VLVTC+RNFRRNRG VSGILKGY+ LSTAIFTDLC+ALFS++P+SFL LL++V
Sbjct: 123 TTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLAVV 182

Query: 184 PFAVCLTAIIFLREIP--FSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLSR 243
           PFAVCLTA+ FLREIP   SA +E E+ +YF + N V+V VAV L  +D+I       S 
Sbjct: 183 PFAVCLTAVFFLREIPPASSAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKTGVFSV 242

Query: 244 IFSIVLLSLLASPLVIPLHSFLK--NRGG----SGEVAEALLAGESAA------------ 303
            F+ +LL LLASP+ IP HSF+K  N G      G + E LL  E AA            
Sbjct: 243 AFASILLFLLASPIAIPFHSFIKSLNYGEQDDLEGRIQEPLLRSEIAAAEKEVIVVAAVA 302

Query: 304 ---DDGVEGKPVIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMNNMGQIGLALGYDD 363
              ++    KPV+GEDHTI+EA+ TV+FW+LFVSFLCGVGTGLAVMNNMGQIGLALGY +
Sbjct: 303 AEEEEKKRKKPVLGEDHTIMEAVLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYTN 362

Query: 364 VSIFISLISIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMALGYIILAIAIPGSL 423
           VSIF+S+ SIWGFFGRILSG+ SE+F+KKAG PRPLWNAASQILMA+GYI++A+A+P SL
Sbjct: 363 VSIFVSMTSIWGFFGRILSGTLSEYFLKKAGTPRPLWNAASQILMAVGYILMALAVPNSL 422

Query: 424 YIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSFLFSGLLAGILYDM 483
           YIGS+VVG+CYGVRLAITVP ASE+FGLK YG+IYN+L+LNLPLGSFLFSGLLAG LYD 
Sbjct: 423 YIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILVLNLPLGSFLFSGLLAGFLYDA 482

Query: 484 EATTTKRGGNTCVGAHCYRLVFVVMACSCVIGFAMDLFLAFRTKRLYSKMRALKR 516
           EAT T  GGNTCVGAHCYRL+F+VMA + VIG  +DL LA+RTK +Y+K+ A K+
Sbjct: 483 EATPTPGGGNTCVGAHCYRLIFIVMALASVIGVGLDLVLAYRTKEIYAKIHASKK 537

BLAST of PI0010481 vs. TAIR 10
Match: AT4G34950.1 (Major facilitator superfamily protein )

HSP 1 Score: 707.6 bits (1825), Expect = 7.3e-204
Identity = 363/551 (65.88%), Postives = 441/551 (80.04%), Query Frame = 0

Query: 7   SPPSSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAF 66
           S  SSSALKWLG V+A+WVQSISGNNYTFSNYS ALKSLMNL+QL+LN+LSVAKD+GKAF
Sbjct: 8   SSSSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDVGKAF 67

Query: 67  GLLAGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMGGNSTTW 126
           G+LAGL+SDRLST ++LLIGS EGL+GYG QWLVVS+ I+P+PYWQMC+FLCMGGNSTTW
Sbjct: 68  GILAGLASDRLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYWQMCVFLCMGGNSTTW 127

Query: 127 MNTGVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFA 186
           MNT VLVTC+RNFRRNRG VSGILKGY+ LSTAIFTDLC+ALFS +P+SFL LLS+VPFA
Sbjct: 128 MNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPASFLVLLSVVPFA 187

Query: 187 VCLTAIIFLREIPFSAD--DETEDFKYFWVLNAVSVAVAVTLLVFDLIPNPNSFLSRIFS 246
           VCLTA+ FLREIP S    ++ E+ KYF V N V+V VAV L  +D+I       S  F+
Sbjct: 188 VCLTAVFFLREIPPSTTFAEDNEESKYFAVFNIVAVVVAVYLQSYDIIGIKTGAFSIAFA 247

Query: 247 IVLLSLLASPLVIPLHSFLKNR------------------GGSGEVAEALLAGESAADDG 306
            +LL LLASP+ +P H+F++++                  G   EV E ++   +AAD+ 
Sbjct: 248 SILLILLASPVAVPFHAFIRSKVHDEQDVEGRIDEPLLRSGSEIEVEETIVGAAAAADNE 307

Query: 307 V----------------------EGKPVIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLA 366
           +                      + +PV+GE+HTI+EAM TV+FW+LFVSFLCGVGTGLA
Sbjct: 308 LPPSLKPLSNEEEENHGTIVTTEKKRPVLGEEHTIMEAMLTVDFWVLFVSFLCGVGTGLA 367

Query: 367 VMNNMGQIGLALGYDDVSIFISLISIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQIL 426
           VMNNMGQIGLALGY DVSIF+S+ SIWGFFGRILSG+ SEHFIKKAG PRPLWNAA+QI+
Sbjct: 368 VMNNMGQIGLALGYTDVSIFVSMTSIWGFFGRILSGTISEHFIKKAGTPRPLWNAAAQII 427

Query: 427 MALGYIILAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPL 486
           MA+GY+++A+A+PGSLYIGS+VVG+CYGVRLAITVP ASE+FGLK YG+IYN+LILN+PL
Sbjct: 428 MAVGYLLMALALPGSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILILNMPL 487

Query: 487 GSFLFSGLLAGILYDMEATTTKRGGNTCVGAHCYRLVFVVMACSCVIGFAMDLFLAFRTK 516
           GSFLFSGLLAG+LYD EAT T  GGNTCVGAHC+R+VF+VMA + +IG  +DL LA+RTK
Sbjct: 488 GSFLFSGLLAGLLYDAEATPTPGGGNTCVGAHCFRIVFIVMAFASIIGVGLDLLLAYRTK 547

BLAST of PI0010481 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 4.4e-84
Identity = 192/564 (34.04%), Postives = 302/564 (53.55%), Query Frame = 0

Query: 15  KWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLAGLSS 74
           +WL  V+A+W+QS +G  Y F + S  +KS +N +Q QL+ L VAKDLG + G LAG  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLS 75

Query: 75  DRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMGGNSTTWMNTGVLVT 134
           + L     LL+GS++ L+GYG  WL+V+ +   LP W MCI + +G N  T+ NT  LV+
Sbjct: 76  EILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVS 135

Query: 135 CLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFAVCLTAIIF 194
            ++NF ++RG V GILKG+  L  AI + + + + S + +S + ++++ P  V +  + F
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPLMFF 195

Query: 195 LREIPFSADDETEDFKYFWVLNAVSVAVA----VTLLVFDLIPNPNSFLSRIFSIVLLSL 254
           +R +       + D   F V+ AV + +A      +LV D I   +S +   F++VL ++
Sbjct: 196 IRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIII-AFTVVLFAI 255

Query: 255 LASPLVIPLHS--FLKNRGGSGEVAEALLAGESAADDG---------------------- 314
           L  P+ IP+ +  F  +      + E LL  +   D G                      
Sbjct: 256 LLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEKPK 315

Query: 315 -------------------------VEGK--------PVIGEDHTIVEAMKTVEFWILFV 374
                                     EG         P  GED T+ +A+   +FW++F 
Sbjct: 316 EVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWLIFF 375

Query: 375 SFLCGVGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRILSGSASEHFIKKAGMP 434
           S L G G+GL V++N+GQ+  +LGYD+  +F+S+ISIW F GRI  G  SE  ++    P
Sbjct: 376 SLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSELIVRDYAYP 435

Query: 435 RPLWNAASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGM 494
           RP+  A +Q++M++G+I  A   PG+++IG++++G+ YG   AI    ASE+FGLK +G 
Sbjct: 436 RPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFGLKKFGA 495

Query: 495 IYNVLILNLPLGSFLFSGLLAGILYDMEATTTKRGG-------NTCVGAHCYRLVFVVMA 511
           +YN L L  P GS +FSGL+A  +YD EA    +G          C G+ CY L  ++M+
Sbjct: 496 LYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSICYFLTSLIMS 555

BLAST of PI0010481 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 307.0 bits (785), Expect = 2.9e-83
Identity = 181/559 (32.38%), Postives = 301/559 (53.85%), Query Frame = 0

Query: 15  KWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLLAGLSS 74
           +WL  V+A+W+QS +G  Y F + S  +KS +N +Q +L+ L VAKDLG + G +AG  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 75  DRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMGGNSTTWMNTGVLVT 134
           + L     LL+G+++ LIGYG  WL+V+ +   LP W MC+ + +G N  T+ NTG LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 135 CLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFAVCLTAIIF 194
            ++NF ++RG V GILKG+  L  AI + + + + S NP+S + ++++ P  V +  + F
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFF 195

Query: 195 LREIPFSADDETEDFKYFWVLNAVSVAVAV----TLLVFDLIPNPNSFLSRIFSIVLLSL 254
           +R +         D   F  +  V + +A      +L+ DL+   ++ ++ +F+IVL  +
Sbjct: 196 IRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVIT-VFTIVLFVI 255

Query: 255 LASPLVIPLHS--FLKNRGGSGEVAEALLAGESAADDGVEGKPVI--------------- 314
           L  P+++P+ +  F +       + E L+      + G++   +I               
Sbjct: 256 LVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLL 315

Query: 315 -----------------------------------GEDHTIVEAMKTVEFWILFVSFLCG 374
                                              GED T+ +A+   +FW++F S L G
Sbjct: 316 PASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLG 375

Query: 375 VGTGLAVMNNMGQIGLALGYDDVSIFISLISIWGFFGRILSGSASEHFIKKAGMPRPLWN 434
            G+GL V++N+GQ+  +LGYD+  + +S+ISIW F GRI  G  SE  ++    PRP+  
Sbjct: 376 SGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVAM 435

Query: 435 AASQILMALGYIILAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVL 494
           A +Q++M++G+I  A   PG++YIG++++G+ YG   AI    ASE+FGLK +G +YN L
Sbjct: 436 AVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNFL 495

Query: 495 ILNLPLGSFLFSGLLAGILYDMEATTTKRG-------GNTCVGAHCYRLVFVVMACSCVI 511
            L  P GS +FSG++A  +YD EA     G          C G+ C+ L  ++M+  C+I
Sbjct: 496 TLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSGFCII 555

BLAST of PI0010481 vs. TAIR 10
Match: AT1G80530.1 (Major facilitator superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 3.8e-83
Identity = 189/545 (34.68%), Postives = 297/545 (54.50%), Query Frame = 0

Query: 10  SSSALKWLGLVSAIWVQSISGNNYTFSNYSAALKSLMNLSQLQLNNLSVAKDLGKAFGLL 69
           S S   W+GL +A WVQ  +G+  TF  YS+ALKS++  SQ Q+  L VA DLG+  GLL
Sbjct: 8   SGSRPPWVGLAAAAWVQVSAGSGSTFPLYSSALKSVLGFSQQQVTILGVACDLGENMGLL 67

Query: 70  AGLSSDRLSTSLLLLIGSIEGLIGYGAQWLVVSQKIKPLPYWQMCIFLCMGGNSTTWMNT 129
            G +S++L    +LLIG+    +G+G  WL VSQ +  LP+W + + L +  NS +W  T
Sbjct: 68  PGYASNKLPPWSMLLIGASSCFLGFGVLWLSVSQIVLGLPFWLLFVALALATNSNSWFGT 127

Query: 130 GVLVTCLRNFRRNRGTVSGILKGYIALSTAIFTDLCSALFSDNPSSFLALLSLVPFAVCL 189
             LVT +RNF  +RG V+G+LKGYI +S A FT L S +   +    L  L++    +CL
Sbjct: 128 ASLVTNMRNFPMSRGPVAGLLKGYIGISGAAFTVLFSMVLHHSAMDLLLFLTVGIPVICL 187

Query: 190 TAIIFLRE-IPFSADDETEDFKYFWVLNAVSVAVAVTLLVFDLIPNP---NSFLSRIFSI 249
           T + F+R  IP + +D +E   YF  L   S+  A  L+V  ++       S L  +   
Sbjct: 188 TVMYFIRPCIPATGEDPSEPM-YFAFLLVTSILFAAYLVVTTVLSEVFILPSILKYVLVA 247

Query: 250 VLLSLLASPLVIPLHSFL-----------------KNRG--------------------- 309
           +++ LL SPL +P+   L                 K  G                     
Sbjct: 248 IMVLLLLSPLAVPIKMTLFRSNAKSSPLGSSDNLAKEEGTHEEPLLTPSTSASNLGPIFE 307

Query: 310 GSGEVAEALLAGESAADDGVEGKPVIGEDHTIVEAMKTVEFWILFVSFLCGVGTGLAVMN 369
           G  E    +L  E+      + KP  GED    +     +FW+L+  +  G+G+G+ V N
Sbjct: 308 GDDESDMEILLAEAEGAVKKKRKPRRGEDFKFGQVFVKADFWLLWFVYFLGMGSGVTVSN 367

Query: 370 NMGQIGLALGYDDVSIFISLISIWGFFGRILSGSASEHFIKKAGMPRPLWNAASQILMAL 429
           N+ QIG A G  D +I + L S + F GR+ SG+ SEHF++   +PR LW  A+Q++M  
Sbjct: 368 NLAQIGFAFGIKDTTILLCLFSFFNFIGRLASGAISEHFVRSRTLPRTLWMGAAQLVMVF 427

Query: 430 GYIILAIAIPGSLYIGSIVVGICYGVRLAITVPMASEVFGLKNYGMIYNVLILNLPLGSF 489
            +++ A+AI  ++Y+ + ++GIC G +  +++   SE+FGL+++G+ +N ++L  PLG+ 
Sbjct: 428 TFLLFAMAIDHTIYVATALIGICMGFQF-LSIATISELFGLRHFGINFNFILLGNPLGAT 487

Query: 490 LFSGLLAGILYDMEATTTKRGGNTCVGAHCYRLVFVVMACSCVIGFAMDLFLAFRTKRLY 513
           +FS +LAG +YD EA   K+G  TC+G  C+R+ F+V+A  C +G  + + L  R + +Y
Sbjct: 488 IFSAILAGYIYDKEA--DKQGKMTCIGPDCFRVTFLVLAGVCGLGTLLSIILTVRIRPVY 547