PI0010402 (gene) Melon (PI 482460) v1

Overview


Name	PI0010402
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Retrovirus-related Pol polyprotein
Location	chr04: 27833254 .. 27833556 (+)
RNA-Seq Expression	PI0010402
Synteny	PI0010402

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGTCACATGGCATAGTTTTAGGCCATTTAGTATCTTCCAAAGGAATAGAAGTAGATAAAGCAAAAATTGACATTATCCAAAAATTGTCATATCCTACCTGTCTAAAAGACATTAGATCTTTTCTTGGTAGCACCGGTTTTTATAGAAGATTCATTAAAGATTTTTCTAAAATTGCTTTACCTCTAACTTCTCTCCTACAAAAAGACATGCCATTTGTGATAGATGAGAAATGCAAAAAAGCGTTCAATGAACTCAAACAAAGGTTAGCTTCTACTCCTATACTTCAATCTCCTAATTGA

mRNA sequence

ATGGTGTCACATGATAAAGCAAAAATTGACATTATCCAAAAATTGTCATATCCTACCTGTCTAAAAGACATTAGATCTTTTCTTGGTAGCACCGGTTTTTATAGAAGATTCATTAAAGATTTTTCTAAAATTGCTTTACCTCTAACTTCTCTCCTACAAAAAGACATGCCATTTGTGATAGATGAGAAATGCAAAAAAGCGTTCAATGAACTCAAACAAAGGTTAGCTTCTACTCCTATACTTCAATCTCCTAATTGA

Coding sequence (CDS)

ATGGTGTCACATGATAAAGCAAAAATTGACATTATCCAAAAATTGTCATATCCTACCTGTCTAAAAGACATTAGATCTTTTCTTGGTAGCACCGGTTTTTATAGAAGATTCATTAAAGATTTTTCTAAAATTGCTTTACCTCTAACTTCTCTCCTACAAAAAGACATGCCATTTGTGATAGATGAGAAATGCAAAAAAGCGTTCAATGAACTCAAACAAAGGTTAGCTTCTACTCCTATACTTCAATCTCCTAATTGA

Protein sequence

MVSHDKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKCKKAFNELKQRLASTPILQSPN

Homology

[Close]

BLAST of PI0010402 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 5.2e-11
Identity = 33/94 (35.11%), Postives = 55/94 (58.51%), Query Frame = 0


Query: 2   VSHDKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQ-------- 61
           +  D  K+  I ++  PT +K+++ FLG T +YR+FI+D++K+A PLT+L +        
Sbjct: 336 IKADPKKVRAISEMPPPTSVKELKRFLGMTSYYRKFIQDYAKVAKPLTNLTRGLYANIKS 395

Query: 62  ---KDMPFVIDEKCKKAFNELKQRLASTPILQSP 85
                +P  +DE   ++FN+LK  L S+ IL  P
Sbjct: 396 SQSSKVPITLDETALQSFNDLKSILCSSEILAFP 429

[Close]

BLAST of PI0010402 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.2e-10
Identity = 34/79 (43.04%), Postives = 52/79 (65.82%), Query Frame = 0


Query: 8   KIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPF-VIDEKCKK 67
           KI+ IQK   PT  K+I++FLG TG+YR+FI +F+ IA P+T  L+K+M     + +   
Sbjct: 426 KIEAIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFADIAKPMTKCLKKNMKIDTTNPEYDS 485

Query: 68  AFNELKQRLASTPILQSPN 86
           AF +LK  ++  PIL+ P+
Sbjct: 486 AFKKLKYLISEDPILKVPD 504

[Close]

BLAST of PI0010402 vs. ExPASy Swiss-Prot
Match: P10394 (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 3.7e-09
Identity = 30/81 (37.04%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 5   DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
           D  K D+IQ    P      R F+    +YRRFIK+F+  +  +T L +K++PF   ++C
Sbjct: 531 DDKKYDVIQNYPVPHDADSARRFVAFCNYYRRFIKNFADYSRHITRLCKKNVPFEWTDEC 590

Query: 65  KKAFNELKQRLASTPILQSPN 86
           +KAF  LK +L +  +LQ P+
Sbjct: 591 QKAFIHLKSQLINPTLLQYPD 611

[Close]

BLAST of PI0010402 vs. ExPASy Swiss-Prot
Match: P03359 (Gag-Pol polyprotein OS=Woolly monkey sarcoma virus OX=11970 GN=pol PE=3 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 4.9e-09
Identity = 28/74 (37.84%), Postives = 46/74 (62.16%), Query Frame = 0


Query: 12   IQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKCKKAFNEL 71
            + K+  PT  + +R FLG+ GF R +I  F+ +A PL  L ++ +PF+  E+ +KAF+ +
Sbjct: 927  VMKIPPPTTPRQVREFLGTAGFCRLWIPGFASLAAPLYPLTKESIPFIWTEEHQKAFDRI 986

Query: 72   KQRLASTPILQSPN 86
            K+ L S P L  P+
Sbjct: 987  KEALLSAPALALPD 1000

[Close]

BLAST of PI0010402 vs. ExPASy Swiss-Prot
Match: P92523 (Uncharacterized mitochondrial protein AtMg00860 OS=Arabidopsis thaliana OX=3702 GN=AtMg00860 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 6.4e-09
Identity = 33/84 (39.29%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 2   VSHDKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVID 61
           VS D AK++ +     P    ++R FLG TG+YRRF+K++ KI  PLT LL+K+      
Sbjct: 44  VSADPAKLEAMVGWPEPKNTTELRGFLGLTGYYRRFVKNYGKIVRPLTELLKKN-SLKWT 103

Query: 62  EKCKKAFNELKQRLASTPILQSPN 86
           E    AF  LK  + + P+L  P+
Sbjct: 104 EMAALAFKALKGAVTTLPVLALPD 126

[Close]

BLAST of PI0010402 vs. ExPASy TrEMBL
Match: A0A151QL68 (Transposon Ty3-G Gag-Pol polyprotein OS=Cajanus cajan OX=3821 GN=KK1_049186 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.1e-23
Identity = 55/81 (67.90%), Postives = 64/81 (79.01%), Query Frame = 0


Query: 5   DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
           DKAKI++I  L YPTC+K+IRSFLG  GFYRRFIKDFSKIA PL+ LLQKD  F  +E C
Sbjct: 877 DKAKIEVISSLPYPTCVKEIRSFLGHAGFYRRFIKDFSKIAYPLSQLLQKDAEFDFNEAC 936

Query: 65  KKAFNELKQRLASTPILQSPN 86
           + AF+ELK RL S PIL +PN
Sbjct: 937 RSAFDELKSRLVSPPILSAPN 957

[Close]

BLAST of PI0010402 vs. ExPASy TrEMBL
Match: A0A392MXJ5 (Uncharacterized protein (Fragment) OS=Trifolium medium OX=97028 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.1e-23
Identity = 53/81 (65.43%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5   DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
           D AKID+I  L +PTC+++IRSFLG  GFYRRFIKDFSKIALPL++LLQKD+ F  D+KC
Sbjct: 94  DPAKIDVISNLPFPTCVREIRSFLGHAGFYRRFIKDFSKIALPLSNLLQKDIIFTFDDKC 153

Query: 65  KKAFNELKQRLASTPILQSPN 86
           K+AF+ LK+ L STP++Q P+
Sbjct: 154 KRAFDCLKKALTSTPVIQPPD 174

[Close]

BLAST of PI0010402 vs. ExPASy TrEMBL
Match: A0A2U1NRI2 (Reverse transcriptase OS=Artemisia annua OX=35608 GN=CTI12_AA230240 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-22
Identity = 54/81 (66.67%), Postives = 65/81 (80.25%), Query Frame = 0


Query: 5    DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
            DKAK+D+I KL  PT +K IRSFLG  GFYRRFIKDFSKIA PLT LL+KD+PF  D++C
Sbjct: 927  DKAKVDVISKLPPPTNVKGIRSFLGHAGFYRRFIKDFSKIARPLTKLLEKDIPFEFDDEC 986

Query: 65   KKAFNELKQRLASTPILQSPN 86
            +KAF  LK++L  TPI+ SPN
Sbjct: 987  QKAFETLKEKLTCTPIIISPN 1007

[Close]

BLAST of PI0010402 vs. ExPASy TrEMBL
Match: A0A371IAU2 (Retrovirus-related Pol polyprotein (Fragment) OS=Mucuna pruriens OX=157652 GN=pol PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-22
Identity = 53/81 (65.43%), Postives = 64/81 (79.01%), Query Frame = 0


Query: 5   DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
           D+ KID+I  L  PT L+++RSFLG   FYRRFIKDFSKIALPL+ LLQKD+ F+ D+ C
Sbjct: 68  DRVKIDVISSLPNPTSLREVRSFLGHANFYRRFIKDFSKIALPLSKLLQKDVDFIFDQSC 127

Query: 65  KKAFNELKQRLASTPILQSPN 86
             AF ELK+RL STPILQ+PN
Sbjct: 128 VDAFQELKRRLTSTPILQAPN 148

[Close]

BLAST of PI0010402 vs. ExPASy TrEMBL
Match: A0A2K3NDP5 (RT_RNaseH_2 domain-containing protein (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g024451 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.8e-22
Identity = 53/81 (65.43%), Postives = 64/81 (79.01%), Query Frame = 0


Query: 5   DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
           D AKI++   L YP+C+ +IRSFLG  GFY RFIKDFSKIALPL++LLQKD+ F  D+KC
Sbjct: 147 DPAKINVTSNLPYPSCVSEIRSFLGHAGFYMRFIKDFSKIALPLSNLLQKDVTFTFDDKC 206

Query: 65  KKAFNELKQRLASTPILQSPN 86
           KKAF+ LK  L STPI+Q PN
Sbjct: 207 KKAFDCLKMALTSTPIIQPPN 227

[Close]

BLAST of PI0010402 vs. NCBI nr
Match: XP_021603241.1 (uncharacterized protein LOC110608326 [Manihot esculenta])

HSP 1 Score: 122.1 bits (305), Expect = 2.3e-24
Identity = 53/81 (65.43%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5   DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
           DKAK+D+I+ L YPTC++DIRSFLG  GFYRRFIKDFS+IA PL  LLQKD+PF  D +C
Sbjct: 450 DKAKVDVIKDLPYPTCIRDIRSFLGHAGFYRRFIKDFSRIAQPLCKLLQKDVPFNFDNEC 509

Query: 65  KKAFNELKQRLASTPILQSPN 86
           ++AFN LK++L + PI+Q+PN
Sbjct: 510 REAFNTLKEKLVAAPIIQAPN 530

[Close]

BLAST of PI0010402 vs. NCBI nr
Match: XP_028802716.1 (uncharacterized protein LOC114757797 [Prosopis alba])

HSP 1 Score: 120.2 bits (300), Expect = 8.8e-24
Identity = 55/81 (67.90%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5   DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
           DKAKIDII  L YP+C+KD+RSFLG  GFYRRFI+DFSKIALPL+ LLQKD+ FV D+ C
Sbjct: 784 DKAKIDIISSLPYPSCVKDVRSFLGHAGFYRRFIQDFSKIALPLSRLLQKDVGFVFDDAC 843

Query: 65  KKAFNELKQRLASTPILQSPN 86
           + A++ELK+RL + PI+Q PN
Sbjct: 844 RYAWDELKKRLTTPPIIQPPN 864

[Close]

BLAST of PI0010402 vs. NCBI nr
Match: XP_028798815.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC114754213 [Prosopis alba])

HSP 1 Score: 119.8 bits (299), Expect = 1.2e-23
Identity = 55/81 (67.90%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5    DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
            DKAKIDII  L YP+C+KD+RSFLG  GFYRRFI+DFSKIALPL+ LLQKD+ FV D+ C
Sbjct: 933  DKAKIDIISSLPYPSCVKDVRSFLGHAGFYRRFIQDFSKIALPLSRLLQKDVGFVFDDAC 992

Query: 65   KKAFNELKQRLASTPILQSPN 86
            + A++ELK+RL + PI+Q PN
Sbjct: 993  RCAWDELKKRLTTPPIIQPPN 1013

[Close]

BLAST of PI0010402 vs. NCBI nr
Match: XP_028797073.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC114752508 [Prosopis alba])

HSP 1 Score: 119.8 bits (299), Expect = 1.2e-23
Identity = 55/81 (67.90%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5    DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
            DKAKIDII  L YP+C+KD+RSFLG  GFYRRFI+DFSKIALPL+ LLQKD+ FV D+ C
Sbjct: 962  DKAKIDIISSLPYPSCVKDVRSFLGHAGFYRRFIQDFSKIALPLSRLLQKDVGFVFDDAC 1021

Query: 65   KKAFNELKQRLASTPILQSPN 86
            + A++ELK+RL + PI+Q PN
Sbjct: 1022 RCAWDELKKRLTTPPIIQPPN 1042

[Close]

BLAST of PI0010402 vs. NCBI nr
Match: XP_019183635.1 (PREDICTED: uncharacterized protein LOC109178455 [Ipomoea nil])

HSP 1 Score: 119.8 bits (299), Expect = 1.2e-23
Identity = 55/81 (67.90%), Postives = 66/81 (81.48%), Query Frame = 0


Query: 5   DKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVIDEKC 64
           DKAK+DII+ L YPT ++++RSFLG  GFYRRFIKDFSKIALPL  LLQK+ PF  D+KC
Sbjct: 379 DKAKVDIIESLPYPTTVREVRSFLGHAGFYRRFIKDFSKIALPLCKLLQKETPFEFDKKC 438

Query: 65  KKAFNELKQRLASTPILQSPN 86
           K AF+ELK+ L S PI+Q PN
Sbjct: 439 KGAFDELKELLTSAPIIQPPN 459

[Close]

BLAST of PI0010402 vs. TAIR 10
Match: ATMG00860.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 4.5e-10
Identity = 33/84 (39.29%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 2   VSHDKAKIDIIQKLSYPTCLKDIRSFLGSTGFYRRFIKDFSKIALPLTSLLQKDMPFVID 61
           VS D AK++ +     P    ++R FLG TG+YRRF+K++ KI  PLT LL+K+      
Sbjct: 44  VSADPAKLEAMVGWPEPKNTTELRGFLGLTGYYRRFVKNYGKIVRPLTELLKKN-SLKWT 103

Query: 62  EKCKKAFNELKQRLASTPILQSPN 86
           E    AF  LK  + + P+L  P+
Sbjct: 104 EMAALAFKALKGAVTTLPVLALPD 126

The following BLAST results are available for this feature:

BLAST of PI0010402 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8I7P9	5.2e-11	35.11	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]
P04323	1.2e-10	43.04	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
P10394	3.7e-09	37.04	Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaste...	[more]
P03359	4.9e-09	37.84	Gag-Pol polyprotein OS=Woolly monkey sarcoma virus OX=11970 GN=pol PE=3 SV=2	[more]
P92523	6.4e-09	39.29	Uncharacterized mitochondrial protein AtMg00860 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of PI0010402 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A151QL68	2.1e-23	67.90	Transposon Ty3-G Gag-Pol polyprotein OS=Cajanus cajan OX=3821 GN=KK1_049186 PE=4...	[more]
A0A392MXJ5	2.1e-23	65.43	Uncharacterized protein (Fragment) OS=Trifolium medium OX=97028 PE=4 SV=1	[more]
A0A2U1NRI2	1.4e-22	66.67	Reverse transcriptase OS=Artemisia annua OX=35608 GN=CTI12_AA230240 PE=4 SV=1	[more]
A0A371IAU2	1.4e-22	65.43	Retrovirus-related Pol polyprotein (Fragment) OS=Mucuna pruriens OX=157652 GN=po...	[more]
A0A2K3NDP5	1.8e-22	65.43	RT_RNaseH_2 domain-containing protein (Fragment) OS=Trifolium pratense OX=57577 ...	[more]

BLAST of PI0010402 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_021603241.1	2.3e-24	65.43	uncharacterized protein LOC110608326 [Manihot esculenta]	[more]
XP_028802716.1	8.8e-24	67.90	uncharacterized protein LOC114757797 [Prosopis alba]	[more]
XP_028798815.1	1.2e-23	67.90	LOW QUALITY PROTEIN: uncharacterized protein LOC114754213 [Prosopis alba]	[more]
XP_028797073.1	1.2e-23	67.90	LOW QUALITY PROTEIN: uncharacterized protein LOC114752508 [Prosopis alba]	[more]
XP_019183635.1	1.2e-23	67.90	PREDICTED: uncharacterized protein LOC109178455 [Ipomoea nil]	[more]

BLAST of PI0010402 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00860.1	4.5e-10	39.29	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 6..85 e-value: 2.8E-27 score: 96.5
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 4..74
None	No IPR available	PANTHER	PTHR24559:SF345	SUBFAMILY NOT NAMED	coord: 4..74
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 2..85

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0010402.1	PI0010402.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding