PI0010257 (gene) Melon (PI 482460) v1

BLAST of PI0010257 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.1e-143
Identity = 257/528 (48.67%), Postives = 350/528 (66.29%), Query Frame = 0

Query: 28  FSSVSSILFGSFFSSDKREFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLL 87
           +S V S+   S        F ++ATDL  +  YDY+++GGGT+GCPLA TLS K+ VL+L
Sbjct: 22  YSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVL 81

Query: 88  ERGSDPNKYPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFY 147
           ERGS P  YP+VL   G       EDDGKTP +RFVSEDG++N+RGR+LGG SM+NAG Y
Sbjct: 82  ERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVY 141

Query: 148 SRGHREFFESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFD 207
           +R +   + ++GVDWDM LV K YEWVE+T+V +P  + WQ+   +A LE G+ P++GF 
Sbjct: 142 ARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFS 201

Query: 208 LRHLVGTKTGGSIFDTKGNRHGAVELLNKANPKNLKVAIEATVQRILFS----------- 267
           L H  GT+  GS FD KG RH A ELLNK N  NL+V + A+V++I+FS           
Sbjct: 202 LDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVI 261

Query: 268 ----------------GEIFLSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQS 327
                           GE+ +SAG IG+PQLLLLSGVGP+S+LSSL +PVVL  P+VGQ 
Sbjct: 262 YRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQF 321

Query: 328 MTDNPRFGTNIVLPFQVFPSSGKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSI- 387
           + DNPR   NI+ P  + P+   ++GI  D  ++Q   S  PF  PP FS  P     + 
Sbjct: 322 LHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTPP-FSFFPSTSYPLP 381

Query: 388 NPTLATFFGKFSEVHSTGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFK 447
           N T A F  K +   S GSL L SS +V+ SP V+FNYYS+P DL+ CV G++K+G++  
Sbjct: 382 NSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLS 441

Query: 448 TPTMEKIKTQDLEGNKGFMFLGVPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD 507
           T  ++  K +DL G +GF  LG+PLP++  +D+A E +C+++VA+YWHYHGGCLVGKV+D
Sbjct: 442 TDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLD 501

Query: 508 SNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQRSS 528
            +++V GI+ LRVVDGSTF  +P ++P    +MLGRYVG+KI+Q+RS+
Sbjct: 502 GDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERSA 546

BLAST of PI0010257 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.8e-141
Identity = 257/529 (48.58%), Postives = 344/529 (65.03%), Query Frame = 0

Query: 28  FSSVSSILFGSFFSSDKREFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLL 87
           +S V S+   S        FV++ATD   +  YDYI++GGGTAGCPLA TLS+ +SVL+L
Sbjct: 22  YSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVL 81

Query: 88  ERGSDPNKYPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFY 147
           ERGS P +YP++L   G       EDDGKTP +RFVSEDG++N+RGR+LGG SM+NAG Y
Sbjct: 82  ERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVY 141

Query: 148 SRGHREFFESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFD 207
            R +  FF   G++WDM LV + YEWVE+T+V +P  + WQ    +A LE GI+P+NGF 
Sbjct: 142 VRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFS 201

Query: 208 LRHLVGTKTGGSIFDTKGNRHGAVELLNKANPKNLKVAIEATVQRILFS----------- 267
           + HL GT+  GS FD  G RH + ELLNK +P NL+VA++A V++I+FS           
Sbjct: 202 VDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTSGVTAIGV 261

Query: 268 -----------------GEIFLSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQ 327
                            GE+ LSAG IGSPQLLLLSGVGP+S+L+SL + VV   P+VGQ
Sbjct: 262 IYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQ 321

Query: 328 SMTDNPRFGTNIVLPFQVFPSSGKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSI 387
            + DNPR   NI+ P  +  S+  ++GI  D  ++Q   S  PF  PP FS  P     +
Sbjct: 322 YIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLPFDTPP-FSFFPTTSYPL 381

Query: 388 -NPTLATFFGKFSEVHSTGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVF 447
            N T A    K     S G++ LNSS DV+  P V+FNYYS+  DL+ CV G++KLG+V 
Sbjct: 382 PNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVL 441

Query: 448 KTPTMEKIKTQDLEGNKGFMFLGVPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVV 507
            T  +E  K +DL G  GF  LG+PLPEN  +D+A E +C+++VA+YWHYHGGCLVGKV+
Sbjct: 442 STDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVL 501

Query: 508 DSNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQRSS 528
           D  ++V GI  LRVVDGSTF  +P ++P    +MLGRY+G++I+Q+RS+
Sbjct: 502 DDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERSA 547

BLAST of PI0010257 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 3.9e-141
Identity = 253/528 (47.92%), Postives = 348/528 (65.91%), Query Frame = 0

Query: 28  FSSVSSILFGSFFSSDKREFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLL 87
           +S V S+   S        F ++ATDL  +  YDY+++GGGT+GCPLA TLS K+ VL+L
Sbjct: 22  YSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVL 81

Query: 88  ERGSDPNKYPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFY 147
           ERGS P  YP+VL   G       EDDGKTP +RFVSEDG++N+RGR+LGG S++NAG Y
Sbjct: 82  ERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVY 141

Query: 148 SRGHREFFESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFD 207
           +R +   + ++GVDWDM LV + YEWVE+T+V +P  + WQ+  ++A LE G+ P++GF 
Sbjct: 142 ARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFS 201

Query: 208 LRHLVGTKTGGSIFDTKGNRHGAVELLNKANPKNLKVAIEATVQRILFS----------- 267
           L H  GT+  GS FD KG RH A ELLNK N  NL+V + A+V++I+FS           
Sbjct: 202 LDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVI 261

Query: 268 ----------------GEIFLSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQS 327
                           GE+ +SAG IG+PQLLLLSGVGP+S+LSSL +PVVL  P+VGQ 
Sbjct: 262 YRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQF 321

Query: 328 MTDNPRFGTNIVLPFQVFPSSGKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSI- 387
           + DNPR   NI+ P  + P+   ++GI  D  ++Q   S  PF  PP F   P     + 
Sbjct: 322 LHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTPP-FGFFPSASYPLP 381

Query: 388 NPTLATFFGKFSEVHSTGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFK 447
           N T A F  K +   S GSL L SS +V+ SP V+FNYYS+  DL+ CV G++K+G++  
Sbjct: 382 NSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLS 441

Query: 448 TPTMEKIKTQDLEGNKGFMFLGVPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD 507
           T  ++  K +DL G +GF  LG+PLP++  +D+A E +C+++VA+YWHYHGGCLVGKV+D
Sbjct: 442 TDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLD 501

Query: 508 SNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQRSS 528
            +++V GI  LRVVDGSTF  +P ++P    +MLGRYVG+KI+Q+RS+
Sbjct: 502 GDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERSA 546

BLAST of PI0010257 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 3.1e-138
Identity = 250/511 (48.92%), Postives = 333/511 (65.17%), Query Frame = 0

Query: 46  EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 105
           +FV+NA DL  +  YDYI++GGGT+GCPLA TLS+ +SVL+LERG+   +YP+ L   G 
Sbjct: 41  KFVYNAVDLELEGSYDYIIVGGGTSGCPLAATLSANYSVLVLERGTIATEYPNTLTVDGF 100

Query: 106 SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMK 165
           +     +DDGKTP +RFVSEDG++N+R RILGG +++NAG Y+R +  F+ ++GV+WD+ 
Sbjct: 101 AYNLQQQDDGKTPVERFVSEDGIDNVRSRILGGTTIINAGVYARANESFYNNSGVEWDLD 160

Query: 166 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKG 225
           LV +AYEWVE+ +V +P  + WQ+   +A LE G+ PDNGF L H  GT+  GS FD  G
Sbjct: 161 LVNEAYEWVEDAIVYKPSNQSWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSG 220

Query: 226 NRHGAVELLNKANPKNLKVAIEATVQRILFS----------------------------G 285
            RH + ELLNK +P NLKVA+EA VQ+I+FS                            G
Sbjct: 221 TRHASDELLNKGDPDNLKVAVEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKG 280

Query: 286 EIFLSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQV 345
           E+ LSAG +G+PQLLLLSGVGP+S+L+SL + VV   P+VGQ + DNPR   NI+ P  +
Sbjct: 281 EVILSAGTLGTPQLLLLSGVGPESYLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPI 340

Query: 346 FPSSGKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSI-NPTLATFFGKFSEVHST 405
            PS+  ++GI  D  ++Q   S  PF  PP FSL P     + N T A    K     S 
Sbjct: 341 EPSTVTVLGITSD--FYQCSLSSLPFDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSA 400

Query: 406 GSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKG 465
           GSL L SS +V  +P V+FNY S P DL  CV G++K+G    T  ++  K  DL G  G
Sbjct: 401 GSLTLQSSSNVSVAPNVKFNYCSDPVDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDG 460

Query: 466 FMFLGVPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGS 525
           F  LG PLPEN  +D+A E++C+ TVA+YWHYHGG +VGKV+D N++V GI  LRVVDGS
Sbjct: 461 FNILGTPLPENQTDDAAFEKFCRDTVASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGS 520

Query: 526 TFSVSPGTNPMATLMMLGRYVGLKIVQQRSS 528
           TF  +P ++P    +MLGRYVG KIVQ+RS+
Sbjct: 521 TFPATPASHPQGFYLMLGRYVGTKIVQERSA 548

BLAST of PI0010257 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 3.8e-136
Identity = 253/528 (47.92%), Postives = 337/528 (63.83%), Query Frame = 0

Query: 28  FSSVSSILFGSFFSSDKREFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLL 87
           +S V S+   S       +FV+NATD   +  YDYIVIGGGT+GCPLA TLS K+ VLLL
Sbjct: 22  YSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATLSEKYKVLLL 81

Query: 88  ERGSDPNKYPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFY 147
           ERG+   +YP+ L   G +     +DDGKTP +RFVSEDG++N+R RILGG +++NAG Y
Sbjct: 82  ERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSEDGIDNVRARILGGTTIINAGVY 141

Query: 148 SRGHREFFESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFD 207
           +R +  F+   G++WD+ LV K YEWVE+ +V +P  + WQ+      LE GI+PDNGF 
Sbjct: 142 ARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEAGILPDNGFS 201

Query: 208 LRHLVGTKTGGSIFDTKGNRHGAVELLNKANPKNLKVAIEATVQRILFS----------- 267
           L H  GT+  GS FD  G RH A ELLNK +P NL VA++A+V++ILFS           
Sbjct: 202 LDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVEKILFSSNTSNLSAIGV 261

Query: 268 -----------------GEIFLSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQ 327
                            GE+ +SAG IG+PQLLLLSGVGP+S+LSSL + VV   P+VGQ
Sbjct: 262 IYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITVVQPNPYVGQ 321

Query: 328 SMTDNPRFGTNIVLPFQVFPSSGKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSI 387
            + +NPR   N   P  +  S   ++GI  D  Y+Q   S  PF  PP FSL P     +
Sbjct: 322 FLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLPFSTPP-FSLFPTTSYPL 381

Query: 388 -NPTLATFFGKFSEVHSTGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVF 447
            N T A    +     S GS+ LNSS DV+ +P ++FNYYS+  DLA CV G++KLGD+ 
Sbjct: 382 PNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTDLANCVSGMKKLGDLL 441

Query: 448 KTPTMEKIKTQDLEGNKGFMFLGVPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVV 507
           +T  +E  K +D+ G  GF +LGVPLPEN  +D++ E +C   VA+YWHYHGG LVGKV+
Sbjct: 442 RTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHGGSLVGKVL 501

Query: 508 DSNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQRS 527
           D +++V+GI+ LRVVD STF   P ++P    +MLGRYVGL+I+Q+RS
Sbjct: 502 DDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYVGLQILQERS 546

BLAST of PI0010257 vs. ExPASy TrEMBL
Match: A0A1S3BSS8 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.7e-256
Identity = 454/542 (83.76%), Postives = 475/542 (87.64%), Query Frame = 0

Query: 16  NSSFVFFFPFTWFSSVSSILFGSFFSSDKR------EFVHNATDLPTKEEYDYIVIGGGT 75
           NSSFVF    T FS VSSI  G  F   ++      +FVHN  DLP KE+YDYI+IGGGT
Sbjct: 2   NSSFVFSISLTRFSLVSSIWSGPPFLPKRKGNVSYMKFVHNGIDLPAKEKYDYIIIGGGT 61

Query: 76  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVE 135
           AGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQGLSKAF TEDDGKTPFQRFVSEDGVE
Sbjct: 62  AGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGVE 121

Query: 136 NIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQA 195
           NIRGR+LGGGSMVN GFYSRGH+EFFESAGVDWDM+LVEKAYEWVEETVVSQPILKDWQA
Sbjct: 122 NIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQPILKDWQA 181

Query: 196 AFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKGNRHGAVELLNKANPKNLKVAIEAT 255
           AFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFD KGNRHGAVELLNKANPKNLKVAIE T
Sbjct: 182 AFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIEVT 241

Query: 256 VQRILFS------------------------GEIFLSAGAIGSPQLLLLSGVGPKSHLSS 315
           VQRILFS                        GEI LSAGAIGSPQLLLLSG+GPKSHLSS
Sbjct: 242 VQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSHLSS 301

Query: 316 LKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSSGKIVGILQDNIYFQSLASPSPFLV 375
           LKLPVVLHQP+VG+SM+DNPRFG ++V+ FQV P++ K+VGILQDNIYFQSLA PSPFLV
Sbjct: 302 LKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSPFLV 361

Query: 376 PPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKLNSSIDVKKSPIVQFNYYSHPDDLA 435
           PPTFSLLP  P+SIN TLA F GKFSEV+S GSLKLNSSIDVKKSPIVQFNYYSHP DLA
Sbjct: 362 PPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPGDLA 421

Query: 436 RCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLGVPLPENLWNDSAVEEYCKKTVATY 495
           RCVK VRKLGDVFKTPTMEKIKTQDLEGNK FMFLG+PLPENLWNDSAVEEYCKKTVATY
Sbjct: 422 RCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTVATY 481

Query: 496 WHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQR 528
           WHYHGGCLVGKVVD NYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQR
Sbjct: 482 WHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQR 541

BLAST of PI0010257 vs. ExPASy TrEMBL
Match: A0A0A0LZU8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G627390 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.2e-255
Identity = 437/494 (88.46%), Postives = 457/494 (92.51%), Query Frame = 0

Query: 46  EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 105
           +FVHNATDLP KEE+DYI+IGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL
Sbjct: 38  KFVHNATDLPRKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 97

Query: 106 SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMK 165
            KAFDTEDDGKTPFQ FVSEDGVENIRGR+LGGGSMVNAGFYSR H+EFFESAGVDWDM+
Sbjct: 98  LKAFDTEDDGKTPFQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDME 157

Query: 166 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKG 225
           LVEKAYEWVEE+VVSQPILKDWQ+AFRSALLEGGIVPDNGFDLRHLVGTKTGGSI D KG
Sbjct: 158 LVEKAYEWVEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKG 217

Query: 226 NRHGAVELLNKANPKNLKVAIEATVQRILFS------------------------GEIFL 285
           NRHGAVELLNKA+PKNLKVAIEATV+RILFS                        GEIFL
Sbjct: 218 NRHGAVELLNKADPKNLKVAIEATVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFL 277

Query: 286 SAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSS 345
           SAGAIGSPQLLLLSG+GPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNI+LPFQV P+S
Sbjct: 278 SAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTS 337

Query: 346 GKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKL 405
           GK+VGILQDNIYFQS+ASPSPFLVPPTFSLLPPHP+SINPTLA FFGKFSEVHS GSLKL
Sbjct: 338 GKVVGILQDNIYFQSIASPSPFLVPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKL 397

Query: 406 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLG 465
           NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPT+EKIKTQ+L+GNKGFMFLG
Sbjct: 398 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLG 457

Query: 466 VPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVS 516
           +PLP NLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD NYKVI  ENLRVVDGSTFSVS
Sbjct: 458 LPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVS 517

BLAST of PI0010257 vs. ExPASy TrEMBL
Match: A0A1S3BSR3 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 3.0e-253
Identity = 439/506 (86.76%), Postives = 458/506 (90.51%), Query Frame = 0

Query: 46  EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 105
           +FVHN  DLP KE+YDYI+IGGGTAGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQGL
Sbjct: 38  KFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGL 97

Query: 106 SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMK 165
           SKAF TEDDGKTPFQRFVSEDGVENIRGR+LGGGSMVN GFYSRGH+EFFESAGVDWDM+
Sbjct: 98  SKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDME 157

Query: 166 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKG 225
           LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFD KG
Sbjct: 158 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKG 217

Query: 226 NRHGAVELLNKANPKNLKVAIEATVQRILFS------------------------GEIFL 285
           NRHGAVELLNKANPKNLKVAIE TVQRILFS                        GEI L
Sbjct: 218 NRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVL 277

Query: 286 SAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSS 345
           SAGAIGSPQLLLLSG+GPKSHLSSLKLPVVLHQP+VG+SM+DNPRFG ++V+ FQV P++
Sbjct: 278 SAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTA 337

Query: 346 GKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKL 405
            K+VGILQDNIYFQSLA PSPFLVPPTFSLLP  P+SIN TLA F GKFSEV+S GSLKL
Sbjct: 338 AKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKL 397

Query: 406 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLG 465
           NSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDLEGNK FMFLG
Sbjct: 398 NSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLG 457

Query: 466 VPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVS 525
           +PLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD NYKVIGIENLRVVDGSTFSVS
Sbjct: 458 LPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVS 517

Query: 526 PGTNPMATLMMLGRYVGLKIVQQRSS 528
           PGTNPMATLMMLGRYVGLKIVQQRSS
Sbjct: 518 PGTNPMATLMMLGRYVGLKIVQQRSS 543

BLAST of PI0010257 vs. ExPASy TrEMBL
Match: A0A1S3BTY8 ((R)-mandelonitrile lyase 1-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 3.0e-253
Identity = 439/506 (86.76%), Postives = 458/506 (90.51%), Query Frame = 0

Query: 46  EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 105
           +FVHN  DLP KE+YDYI+IGGGTAGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQGL
Sbjct: 2   KFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGL 61

Query: 106 SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMK 165
           SKAF TEDDGKTPFQRFVSEDGVENIRGR+LGGGSMVN GFYSRGH+EFFESAGVDWDM+
Sbjct: 62  SKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDME 121

Query: 166 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKG 225
           LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFD KG
Sbjct: 122 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKG 181

Query: 226 NRHGAVELLNKANPKNLKVAIEATVQRILFS------------------------GEIFL 285
           NRHGAVELLNKANPKNLKVAIE TVQRILFS                        GEI L
Sbjct: 182 NRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVL 241

Query: 286 SAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSS 345
           SAGAIGSPQLLLLSG+GPKSHLSSLKLPVVLHQP+VG+SM+DNPRFG ++V+ FQV P++
Sbjct: 242 SAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTA 301

Query: 346 GKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKL 405
            K+VGILQDNIYFQSLA PSPFLVPPTFSLLP  P+SIN TLA F GKFSEV+S GSLKL
Sbjct: 302 AKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKL 361

Query: 406 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLG 465
           NSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDLEGNK FMFLG
Sbjct: 362 NSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLG 421

Query: 466 VPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVS 525
           +PLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD NYKVIGIENLRVVDGSTFSVS
Sbjct: 422 LPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVS 481

Query: 526 PGTNPMATLMMLGRYVGLKIVQQRSS 528
           PGTNPMATLMMLGRYVGLKIVQQRSS
Sbjct: 482 PGTNPMATLMMLGRYVGLKIVQQRSS 507

BLAST of PI0010257 vs. ExPASy TrEMBL
Match: A0A5A7VHS6 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00760 PE=3 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.6e-246
Identity = 427/494 (86.44%), Postives = 446/494 (90.28%), Query Frame = 0

Query: 46  EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 105
           +FVHN  DLP KE+YDYI+IGGGTAGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQGL
Sbjct: 2   KFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGL 61

Query: 106 SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMK 165
           SKAF TEDDGKTPFQRFVSEDGVENIRGR+LGGGSMVN GFYSRGH+EFFESAGVDWDM+
Sbjct: 62  SKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDME 121

Query: 166 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKG 225
           LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFD KG
Sbjct: 122 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKG 181

Query: 226 NRHGAVELLNKANPKNLKVAIEATVQRILFS------------------------GEIFL 285
           NRHGAVELLNKANPKNLKVAIE TVQRILFS                        GEI L
Sbjct: 182 NRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVL 241

Query: 286 SAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSS 345
           SAGAIGSPQLLLLSG+GPKSHLSSLKLPVVLHQP+VG+SM+DNPRFG ++V+ FQV P++
Sbjct: 242 SAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTA 301

Query: 346 GKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKL 405
            K+VGILQDNIYFQSLA PSPFLVPPTFSLLP  P+SIN TLA F GKFSEV+S GSLKL
Sbjct: 302 AKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKL 361

Query: 406 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLG 465
           NSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDLEGNK FMFLG
Sbjct: 362 NSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLG 421

Query: 466 VPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVS 516
           +PLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD NYKVIGIENLRVVDGSTFSVS
Sbjct: 422 LPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVS 481

BLAST of PI0010257 vs. NCBI nr
Match: XP_011650118.2 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61134.2 hypothetical protein Csa_021306 [Cucumis sativus])

HSP 1 Score: 923.3 bits (2385), Expect = 9.3e-265
Identity = 454/506 (89.72%), Postives = 472/506 (93.28%), Query Frame = 0

Query: 46  EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 105
           +FVHNATDLPTKEEYDYI+IGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNE+GL
Sbjct: 38  KFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEKGL 97

Query: 106 SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMK 165
           SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGH+EFFESAGVDWDM+
Sbjct: 98  SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHKEFFESAGVDWDME 157

Query: 166 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKG 225
           LVEKAYEW+EE+VVSQPILKDWQ+AFRSALLEGGIVPDNGFDL HLVGTKTGGSI D KG
Sbjct: 158 LVEKAYEWIEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLSHLVGTKTGGSIIDDKG 217

Query: 226 NRHGAVELLNKANPKNLKVAIEATVQRILFS------------------------GEIFL 285
           NRHGAVELLNKANPKNLKVAIEATVQRILFS                        GEI +
Sbjct: 218 NRHGAVELLNKANPKNLKVAIEATVQRILFSDLSANGVSYLDSKGKLHTTFIHEKGEIIV 277

Query: 286 SAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSS 345
           SAGAIGSPQLLLLSG+GPKSHLSSLKLPVVLHQPHVGQSM+DNPRFGTNIVLPFQV P+S
Sbjct: 278 SAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQSMSDNPRFGTNIVLPFQVVPTS 337

Query: 346 GKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKL 405
           GK+VGILQDNIYFQSLASPSPFLVPPTFSLLPPHP+SINPTLATFFGKFSEVHS GSLKL
Sbjct: 338 GKVVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKL 397

Query: 406 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLG 465
           NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDL+GNKGFMFLG
Sbjct: 398 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLG 457

Query: 466 VPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVS 525
           +PLP NLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD NYKVIG ENLRVVDGSTFS+S
Sbjct: 458 LPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGTENLRVVDGSTFSIS 517

Query: 526 PGTNPMATLMMLGRYVGLKIVQQRSS 528
           PGTNPMAT+MMLGRYVGLKIVQQ SS
Sbjct: 518 PGTNPMATVMMLGRYVGLKIVQQSSS 543

BLAST of PI0010257 vs. NCBI nr
Match: XP_031736583.1 ((R)-mandelonitrile lyase 1 [Cucumis sativus] >KAE8651658.1 hypothetical protein Csa_021303 [Cucumis sativus])

HSP 1 Score: 908.7 bits (2347), Expect = 2.4e-260
Identity = 446/506 (88.14%), Postives = 469/506 (92.69%), Query Frame = 0

Query: 46  EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 105
           +FVHNATDLP KEE+DYI+IGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL
Sbjct: 38  KFVHNATDLPRKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 97

Query: 106 SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMK 165
            KAFDTEDDGKTPFQ FVSEDGVENIRGR+LGGGSMVNAGFYSR H+EFFESAGVDWDM+
Sbjct: 98  LKAFDTEDDGKTPFQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDME 157

Query: 166 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKG 225
           LVEKAYEWVEE+VVSQPILKDWQ+AFRSALLEGGIVPDNGFDLRHLVGTKTGGSI D KG
Sbjct: 158 LVEKAYEWVEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKG 217

Query: 226 NRHGAVELLNKANPKNLKVAIEATVQRILFS------------------------GEIFL 285
           NRHGAVELLNKA+PKNLKVAIEATV+RILFS                        GEIFL
Sbjct: 218 NRHGAVELLNKADPKNLKVAIEATVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFL 277

Query: 286 SAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSS 345
           SAGAIGSPQLLLLSG+GPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNI+LPFQV P+S
Sbjct: 278 SAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTS 337

Query: 346 GKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKL 405
           GK+VGILQDNIYFQS+ASPSPFLVPPTFSLLPPHP+SINPTLA FFGKFSEVHS GSLKL
Sbjct: 338 GKVVGILQDNIYFQSIASPSPFLVPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKL 397

Query: 406 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLG 465
           NSSIDVKK+PIV+FNYYSHPDDLARCVKGVRKLGDVFKTPT+EKIKTQ+L+GNKGFMFLG
Sbjct: 398 NSSIDVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLG 457

Query: 466 VPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVS 525
           +PLP NLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD NYKVI  ENLRVVDGSTFSVS
Sbjct: 458 LPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVS 517

Query: 526 PGTNPMATLMMLGRYVGLKIVQQRSS 528
           PGTNPMATLMMLGRYVGLKI+QQRSS
Sbjct: 518 PGTNPMATLMMLGRYVGLKILQQRSS 543

BLAST of PI0010257 vs. NCBI nr
Match: XP_008452005.2 (PREDICTED: (R)-mandelonitrile lyase 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 894.8 bits (2311), Expect = 3.6e-256
Identity = 454/542 (83.76%), Postives = 475/542 (87.64%), Query Frame = 0

Query: 16  NSSFVFFFPFTWFSSVSSILFGSFFSSDKR------EFVHNATDLPTKEEYDYIVIGGGT 75
           NSSFVF    T FS VSSI  G  F   ++      +FVHN  DLP KE+YDYI+IGGGT
Sbjct: 2   NSSFVFSISLTRFSLVSSIWSGPPFLPKRKGNVSYMKFVHNGIDLPAKEKYDYIIIGGGT 61

Query: 76  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVE 135
           AGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQGLSKAF TEDDGKTPFQRFVSEDGVE
Sbjct: 62  AGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGVE 121

Query: 136 NIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQA 195
           NIRGR+LGGGSMVN GFYSRGH+EFFESAGVDWDM+LVEKAYEWVEETVVSQPILKDWQA
Sbjct: 122 NIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQPILKDWQA 181

Query: 196 AFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKGNRHGAVELLNKANPKNLKVAIEAT 255
           AFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFD KGNRHGAVELLNKANPKNLKVAIE T
Sbjct: 182 AFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIEVT 241

Query: 256 VQRILFS------------------------GEIFLSAGAIGSPQLLLLSGVGPKSHLSS 315
           VQRILFS                        GEI LSAGAIGSPQLLLLSG+GPKSHLSS
Sbjct: 242 VQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSHLSS 301

Query: 316 LKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSSGKIVGILQDNIYFQSLASPSPFLV 375
           LKLPVVLHQP+VG+SM+DNPRFG ++V+ FQV P++ K+VGILQDNIYFQSLA PSPFLV
Sbjct: 302 LKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSPFLV 361

Query: 376 PPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKLNSSIDVKKSPIVQFNYYSHPDDLA 435
           PPTFSLLP  P+SIN TLA F GKFSEV+S GSLKLNSSIDVKKSPIVQFNYYSHP DLA
Sbjct: 362 PPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPGDLA 421

Query: 436 RCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLGVPLPENLWNDSAVEEYCKKTVATY 495
           RCVK VRKLGDVFKTPTMEKIKTQDLEGNK FMFLG+PLPENLWNDSAVEEYCKKTVATY
Sbjct: 422 RCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTVATY 481

Query: 496 WHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQR 528
           WHYHGGCLVGKVVD NYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQR
Sbjct: 482 WHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQR 541

BLAST of PI0010257 vs. NCBI nr
Match: XP_008451997.1 (PREDICTED: (R)-mandelonitrile lyase 1-like isoform X3 [Cucumis melo])

HSP 1 Score: 884.0 bits (2283), Expect = 6.3e-253
Identity = 439/506 (86.76%), Postives = 458/506 (90.51%), Query Frame = 0

Query: 46  EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 105
           +FVHN  DLP KE+YDYI+IGGGTAGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQGL
Sbjct: 2   KFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGL 61

Query: 106 SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMK 165
           SKAF TEDDGKTPFQRFVSEDGVENIRGR+LGGGSMVN GFYSRGH+EFFESAGVDWDM+
Sbjct: 62  SKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDME 121

Query: 166 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKG 225
           LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFD KG
Sbjct: 122 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKG 181

Query: 226 NRHGAVELLNKANPKNLKVAIEATVQRILFS------------------------GEIFL 285
           NRHGAVELLNKANPKNLKVAIE TVQRILFS                        GEI L
Sbjct: 182 NRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVL 241

Query: 286 SAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSS 345
           SAGAIGSPQLLLLSG+GPKSHLSSLKLPVVLHQP+VG+SM+DNPRFG ++V+ FQV P++
Sbjct: 242 SAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTA 301

Query: 346 GKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKL 405
            K+VGILQDNIYFQSLA PSPFLVPPTFSLLP  P+SIN TLA F GKFSEV+S GSLKL
Sbjct: 302 AKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKL 361

Query: 406 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLG 465
           NSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDLEGNK FMFLG
Sbjct: 362 NSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLG 421

Query: 466 VPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVS 525
           +PLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD NYKVIGIENLRVVDGSTFSVS
Sbjct: 422 LPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVS 481

Query: 526 PGTNPMATLMMLGRYVGLKIVQQRSS 528
           PGTNPMATLMMLGRYVGLKIVQQRSS
Sbjct: 482 PGTNPMATLMMLGRYVGLKIVQQRSS 507

BLAST of PI0010257 vs. NCBI nr
Match: XP_008451990.1 (PREDICTED: (R)-mandelonitrile lyase 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 884.0 bits (2283), Expect = 6.3e-253
Identity = 439/506 (86.76%), Postives = 458/506 (90.51%), Query Frame = 0

Query: 46  EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 105
           +FVHN  DLP KE+YDYI+IGGGTAGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQGL
Sbjct: 38  KFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGL 97

Query: 106 SKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMK 165
           SKAF TEDDGKTPFQRFVSEDGVENIRGR+LGGGSMVN GFYSRGH+EFFESAGVDWDM+
Sbjct: 98  SKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDME 157

Query: 166 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKG 225
           LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFD KG
Sbjct: 158 LVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKG 217

Query: 226 NRHGAVELLNKANPKNLKVAIEATVQRILFS------------------------GEIFL 285
           NRHGAVELLNKANPKNLKVAIE TVQRILFS                        GEI L
Sbjct: 218 NRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVL 277

Query: 286 SAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSS 345
           SAGAIGSPQLLLLSG+GPKSHLSSLKLPVVLHQP+VG+SM+DNPRFG ++V+ FQV P++
Sbjct: 278 SAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTA 337

Query: 346 GKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSINPTLATFFGKFSEVHSTGSLKL 405
            K+VGILQDNIYFQSLA PSPFLVPPTFSLLP  P+SIN TLA F GKFSEV+S GSLKL
Sbjct: 338 AKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKL 397

Query: 406 NSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLG 465
           NSSIDVKKSPIVQFNYYSHP DLARCVK VRKLGDVFKTPTMEKIKTQDLEGNK FMFLG
Sbjct: 398 NSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLG 457

Query: 466 VPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVS 525
           +PLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVD NYKVIGIENLRVVDGSTFSVS
Sbjct: 458 LPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVS 517

Query: 526 PGTNPMATLMMLGRYVGLKIVQQRSS 528
           PGTNPMATLMMLGRYVGLKIVQQRSS
Sbjct: 518 PGTNPMATLMMLGRYVGLKIVQQRSS 543

BLAST of PI0010257 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 482.3 bits (1240), Expect = 5.1e-136
Identity = 238/511 (46.58%), Postives = 332/511 (64.97%), Query Frame = 0

Query: 47  FVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLS 106
           F+ NATD  +++ YDYI++GGGTAGCPLA TLS  F VLLLERG  P   P+V++  G  
Sbjct: 41  FISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFL 100

Query: 107 KAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFFESAGVDWDMKL 166
                 ++  +P Q F+SE+GV N RGR+LGG S +NAGFYSR  ++FFE++G+ WD+  
Sbjct: 101 TTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSS 160

Query: 167 VEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDTKGN 226
           V ++YEWVE  +V +P L+ WQ A R ALLE G+ P NGF L H VGTK GGS FD  G 
Sbjct: 161 VNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGR 220

Query: 227 RHGAVELLNKANPKNLKVAIEATVQRILFS------------------------------ 286
           RH + +LL  A   N++VA+ ATV+R+L +                              
Sbjct: 221 RHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIR 280

Query: 287 --GEIFLSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIVLP 346
             GE+ LSAGA+GSPQLL LSG+GP+S+LS+  +PV L QPHVG  + DNPR G +IV P
Sbjct: 281 DRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPP 340

Query: 347 FQVFPSSGKIVGILQDNIYFQSLASPSPFLVPPTFSLLPPHPSSINPTLATFFGKFSEVH 406
             +  S  ++VG+ +D  + ++ ++  PF  P     +    S +   + T   K     
Sbjct: 341 VPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPV 400

Query: 407 STGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGN 466
           S G L+L +S DV+ +P+V+FNY+S P DL RCV G RK+G++ ++  M+    ++  GN
Sbjct: 401 SIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGN 460

Query: 467 KGFMFLGVPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVD 526
           + F F+G PLP +  ND  + ++C++TV+T WHYHGG +VGKVVDS+ KVIG+ +LR+VD
Sbjct: 461 RRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVD 520

BLAST of PI0010257 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 399.1 bits (1024), Expect = 5.7e-111
Identity = 239/569 (42.00%), Postives = 312/569 (54.83%), Query Frame = 0

Query: 22  FFPFTWFSSVSSILFGSFFSSDKR---EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTL 81
           F+ +  F ++S  L     SSDK     F+ +AT  PT   YDYI+IGGGTAGCPLA TL
Sbjct: 5   FWSWRLFVALSLFLHSPICSSDKAPNYSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATL 64

Query: 82  SSKFSVLLLERGSDPNKYPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGG 141
           S   SVLLLERG  P   P++        A     +  +P QRFVSEDGV N R R+LGG
Sbjct: 65  SQNASVLLLERGDSPYNNPNITRLSAFGAALSDLSE-SSPSQRFVSEDGVINARARVLGG 124

Query: 142 GSMVNAGFYSRGHREFFESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQAAFRSALLEG 201
           GS +NAGFY+R   ++  + G  WD  L  ++Y+WVE  V  QP +  WQ A R  LLE 
Sbjct: 125 GSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEA 184

Query: 202 GIVPDNGFDLRHLVGTKTGGSIFDTKGNRHGAVELLNKANPKNLKVAIEATVQRILFS-- 261
           GIVP+NGF   H+ GTK GG+IFD  GNRH A +LL  A+PK + V + ATV RILF   
Sbjct: 185 GIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTR 244

Query: 262 -----------------------------GEIFLSAGAIGSPQLLLLSGVGPKSHLSSLK 321
                                         EI LSAG +GSPQLL+LSGVGP + L +  
Sbjct: 245 GTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQN 304

Query: 322 LPVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSSGKIVGILQDNIYFQSLASPS------ 381
           + VV+ QPHVGQ M DNP     +  P  V  S  ++VGI  +  Y ++    +      
Sbjct: 305 ITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGG 364

Query: 382 -----------PFLVPPTFSLLPPHP----SSINPTLATF-FGKFSEVHSTGSLKLNSSI 441
                        +  P  +LL  +     SS  P    F   K     STG L+L +  
Sbjct: 365 GSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTR- 424

Query: 442 DVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLGVPLP 501
           + K +PIV FNY+ HPDDL RCV+G++ +  V ++    + K  D+   +  + L    P
Sbjct: 425 NPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV-SFEYLLNLTASTP 484

Query: 502 ENLWN---------DSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGS 526
            NL             + EE+C+ TV T WHYHGGC+VG+VVD +YKVIGI+ LRV+D S
Sbjct: 485 VNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMS 544

BLAST of PI0010257 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 376.7 bits (966), Expect = 3.0e-104
Identity = 232/554 (41.88%), Postives = 298/554 (53.79%), Query Frame = 0

Query: 38  SFFSSDKREFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLLERGSDP--NK 97
           +F SS    F  N  D      YDYIVIGGGTAGCPLA TLS  FSVL+LERG  P  N 
Sbjct: 45  TFSSSSSSSFSSNGQD----SSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNA 104

Query: 98  YPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHREFF 157
             S L    +  A   +    +  Q FVS DGV N R R+LGGGS +NAGFYSR    F 
Sbjct: 105 NVSFLRNFHIGLA---DISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFV 164

Query: 158 ESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTK 217
           + AG  WD KLV+++Y WVE  +V QP L  WQ A R +LLE G+ P NGF   H+ GTK
Sbjct: 165 KRAG--WDPKLVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTK 224

Query: 218 TGGSIFDTKGNRHGAVELLNKANPKNLKVAIEATVQRILF-------------------- 277
            GG+IFD  G RH A ELL  ANP+ L+V I ATVQ+I+F                    
Sbjct: 225 IGGTIFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGN 284

Query: 278 ----------SGEIFLSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQSMTDNP 337
                       E+ LS+GAIGSPQ+L+LSG+GPK  L  LK+PVVL   HVG+ M DNP
Sbjct: 285 QHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNP 344

Query: 338 RFGTNIVLPFQVFPSSGKIVGILQDNIYFQSLA--SPSPFLV----------PPTFSLLP 397
                +     +  S  + VGI +  +Y ++      SP  +             FS +P
Sbjct: 345 MNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIP 404

Query: 398 PHPSSINPTLATFFGKFSEVH----------------STGSLKL-NSSIDVKKSPIVQFN 457
                   T A       ++H                S G L L N+++D   +P V FN
Sbjct: 405 AKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNVD--DNPSVTFN 464

Query: 458 YYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLGVPL-----PENLWND 517
           Y+ HP DL RCV+ +R +  V  +         D +     + L V       P+ L + 
Sbjct: 465 YFKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDT 524

Query: 518 SAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTFSVSPGTNPMATLM 526
            ++ ++CK TV T WHYHGGCLVGKVV  N KV+G++ LRV+DGSTF  SPGTNP AT+M
Sbjct: 525 KSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMM 584

BLAST of PI0010257 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 364.8 bits (935), Expect = 1.2e-100
Identity = 230/567 (40.56%), Postives = 308/567 (54.32%), Query Frame = 0

Query: 23  FPFTWFSSVSSILFGSFFSSDKR---EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLS 82
           F F  F  V+  +F     SD+     F+ +AT  P    +DYI+IGGGTAGC LA TLS
Sbjct: 6   FRFFQFILVAVFIFHDSCYSDEAGNYRFMKDATLAPKLSHFDYIIIGGGTAGCALAATLS 65

Query: 83  SKFSVLLLERGSDPNKYPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGG 142
              +VL+LERG  P   P+  +    +          +  Q F+SEDGV N R R+LGGG
Sbjct: 66  QNATVLVLERGGSPYDDPAATDIGNFANTL-LNITPNSWSQLFISEDGVFNSRARVLGGG 125

Query: 143 SMVNAGFYSRGHREFFESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQAAFRSALLEGG 202
           +++NAGFYSR   +F   AG  W+   VE AYEWVE+ VV +P +  WQ+AFR  LLE G
Sbjct: 126 TVINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAG 185

Query: 203 IVPDNGFDLRHLVGTKTGGSIFDTKGNRHGAVELLNKANPKNLKVAIEATVQRILFS--- 262
           + P NGF   H+VGTK GG+IFD  G+RH A  LL  ANP  + V + A+V +ILF+   
Sbjct: 186 VTPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHKILFTIKG 245

Query: 263 ------------------------------GEIFLSAGAIGSPQLLLLSGVGPKSHLSSL 322
                                          E+ LSAGAI SPQLL+LSGVGP +HL++ 
Sbjct: 246 NQRPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAY 305

Query: 323 KL-PVVLHQPHVGQSMTDNPRFGTNIVLPFQVFPSSGKIVGILQDNIYFQ--SLASPSPF 382
           ++ PV++ QP VGQ M DNP     I  P  V  S  + VGI +   Y +  S  S S  
Sbjct: 306 RVNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGSALSLSIS 365

Query: 383 LVPPTF---------SLLPPHPSS-----------INPTLATFFGKFSEVHSTGSLKLNS 442
           L    F         + LP    S           +         K +   S G L+L +
Sbjct: 366 LTRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELRN 425

Query: 443 SIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLEGNKGFMFLGVP 502
           + +   +P V FNY+  P+DL +CV+G+  +  V  +    K K   L   +G + L + 
Sbjct: 426 T-NPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYK-YPLASARGLLNLILA 485

Query: 503 LPENL-----WNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGSTF 526
           LP NL      +   +E+YC  TV T +HYHGGC VGKVVD+NYKV+G++ LR++DGSTF
Sbjct: 486 LPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTF 545

BLAST of PI0010257 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 361.7 bits (927), Expect = 1.0e-99
Identity = 225/568 (39.61%), Postives = 307/568 (54.05%), Query Frame = 0

Query: 31  VSSILFGSFFSSDKR---EFVHNATDLPTKEEYDYIVIGGGTAGCPLATTLSSKFSVLLL 90
           + + +F +  S DK     F+ +AT  P    +DYI+IGGGT+GC LA TLS   SVL+L
Sbjct: 14  IITYIFHASCSPDKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVL 73

Query: 91  ERGSDPNKYPSVLNEQGLSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFY 150
           ERG  P   P+  + +  +    +    K+  Q F+SEDGV N R R+LGGGS++NAGFY
Sbjct: 74  ERGGAPYDNPTATDIENFATTL-SNTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFY 133

Query: 151 SRGHREFFESAGVDWDMKLVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFD 210
           +R   E+ +    +W    VE AYEWVE+ V  QP +  WQ AF+  LLE G  P NGF 
Sbjct: 134 TRAGDEYVKE--TEWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFT 193

Query: 211 LRHLVGTKTGGSIFDTKGNRHGAVELLNKANPKNLKVAIEATVQRILFS----------- 270
             H+ GTK GG+IFD  G+RH A +LL  ANP N+ V + A+V +ILF+           
Sbjct: 194 YDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYG 253

Query: 271 --------------------GEIFLSAGAIGSPQLLLLSGVGPKSHLSSLKL-PVVLHQP 330
                                E+ LSAGAIGSPQLL+LSG+GP +HL++  + P+VL  P
Sbjct: 254 VIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHP 313

Query: 331 HVGQSMTDNPRFGTNIVLPFQVFPSSGKIVGILQDNIYFQSLA----------------- 390
            VGQ M DNP     I  P  V  S  ++VGI +   Y +  +                 
Sbjct: 314 MVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVL 373

Query: 391 --------SPSPFLVPPTFSL--LPPHPSSINPTL------ATFFGKFSEVHSTGSLKLN 450
                   S +     PT S   +    + INP L           K +   S G L+L 
Sbjct: 374 NYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR 433

Query: 451 SSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQD--LEGNKGFMFL 510
           ++ +   +P V+FNYY  P+DL  CV+G+  +  V  +    K K  D  + G    M L
Sbjct: 434 NT-NPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLM-L 493

Query: 511 GVPL---PENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDSNYKVIGIENLRVVDGST 526
            VP    P ++ +   + ++C  TV T WHYHGGC VG+VVD NY+V+GI++LRV+DGST
Sbjct: 494 SVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGST 553