PI0010238 (gene) Melon (PI 482460) v1

Overview
NamePI0010238
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Description4-methyl-5-beta-hydroxyethylthiazole kinase
Locationchr06: 2901813 .. 2904936 (+)
RNA-Seq ExpressionPI0010238
SyntenyPI0010238
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGTATATTTGAAAATGTACCAAATTTAATTAGATTGTTAAAATGAGCGAAGGCGATGAATCCAATTCTTGAACAGTGCGAGTAGAGAAAGACAGAGATAACATATCATCAAGCGATGAATTCGAAGCCGGATGAAGACGAACAACAGCGACGGCAATGGGGAACAAGAGCGTGGGCCACACTTGCCGCCATACGAACCCGATCGCCGCTCATCCAATGCATCACCAACTTCGTTTCAATGGATTTAATGGCCAATACTCTCTTATCCGCCGGAGCCTCGCCGGCCATGCTTCATTCCGTCGAGGAGTTGCCGGATTTCACTCCCAACGTTGACGCTCTCTGTATCAATGTCGGCACGCTTTCCCCCGCCTGGTTACCCGCCATGAAGTTCGCCGCTGAACTGGCGGTAAAGGCCGGGAAACCTTGGGTTCTCGATCCGGTGGCGGCCGGAGCATCTAAGTACCGGATGATGGCTTGTTTGGAGCTTATGAATTTGAAGCCCACCGTTGTTAGAGGGAATGGGTCAGAAATAATCGCTCTGTCTAAAGCTTCTTCGGATTCCAGTATGGTAAGATGCAATTTCATTTTACACTGCAAATTGATATAATTTCAGTACTATTATTCCTTCTTCTTTACCTCAAATTCCTACATCCAAAGCTACAAACTAGGTTACATCCATCTAGTAGTTCAATTCTAGGGGGAGCTTATTTAACTTGGGCATGTCGATTACTTTTCCCTTAATATGCCTAAAATTTCAAGCCCAAGTGTTTGAAGTTTCAAATTTCGAGCATCAGCTCGTCTTTAAAGCCTAGCTTTGTTATCTATGTATTTATATTTACTTTTTTGTTCTTTGAGCGTTGGTCTCTTTTTATTATTTTATCCAAAAGTTTTGTTTTCTTTCATATAACAATCTATGTCTTTCATGGAATCAAGAGTTATAACATTTTAAGTGACAAAATCATTTTTTATTTTCTTTACTCTATAGTTAGTTATGCAGGACCCTTTTTCTTTGTCAAACTGGATTGTTAAATCCTATCATCCCACCCAAAAGGATGAATTATGGTAAATTTAATCACATCAAAATTTTTAACACTTTTTCTTACTCGTAGGTTTGAAAATTGATAAAAGGTCTCATAAGTGAAAATCAATATTAATTGGAAAGATAACAACATTACAGGATTTAAAGAGAGAACTTTTTGCTTCACCATCTTGTTAAATCACTAATCAACTCAAAAGCTTAAACAAATGGGTTATGATAAATTTAATTATATCGATATTTCAACATTCATCTTTATTTCTTTGCCTCGATATCAATTTGAAGGGTGTGGATAGCTGCCACGACTCGGTGGATGCTGTAGAAGCTGCTAAATCATTAGCCCAATCCAGTGGAGCCATAGTAGCAGTATCAGGAGCCGTGGACATAGTTACAGACGGGAACCAAGTTATTGGTGCTCGTAATGGTGTGGCCATGATGCAAAAGATTACAGCAACTGGATGCTCTGTAACAGCTCTTATTGCTGCCTTTCTTGCTGTTGATCAACTTCATGCTTTAGAAGCAACAGCCTCGGCTTTATCAATATTTGGAATTGCAGGAGAGATGGGAATGGACGTTGCAAAGGGCCCTGCATCACTACGAACTCATTTGATTGATTCACTTTATGGGCTCGATGAAGCTTCTATAATCTCAAGAATAAGGATCTCCAGCTTGTGATTAAATTTTGGACGCGGTTTTATGTATGGTATGCCTAGTAACTAGTCTTGAACTCTTTTGAGTTTGCACAGTGCTTTAATGTTCAATAATATTGAAAAGATTGGCTACCCTACACTTTTCGTTTACATATCCATTCAAGGTTCCATTCTTTTCGGTGAAGTTATATACATCTTAATCTGATCGACCTCATAAATTTGTTTCGTTCTTTTGATGCTGTTTTTAACTATTTTTATAATCCGGTTTTATCATGAAATTCAACTAAATTCACTCATGGTATGATCACAACAGAATTACAACTGAGAAATATATAAATTTCATAAAACTGGATTACATTTTTTGACTTGCAGCTGCATTTGATAGAAACTACAGACCATGGTTGCATTCATCACCACACATATATCGCGTTGTTCTTTGTTCACCACACTGGTTTTCAGTAGGTTAGATGTACAAGGACCCTCAGCAATGTTTCTGCTCCATCTGCCACGTGCTGGCCCGGAGGAATGAGGGATCGCACCTCTGCAATCCCACGTGCATTCTTGAACTTGCCAGTTCCTCCAGTCACTGACAAATGCGATGATGGGCTACCAATCCTGAATACTCCATAGAAGTTGAGACTGTCACCATATTCTCCTCCTTCAACCATGGCTGTAAATGCCATCATCTGCGTAGTTCCATCAGCCGAACTAGCCACATATACCCCTTGTGCCTGGCCAATTGACTGTGAGCCCAACTCTGGTGCGGTGGTCAAGACGTCATCAATGACTGTAATCGTTCCAAAGCCTAGCCCCAACCCATCAGGTCCCAGTTGGGTCTGGGGGACATTTTGTGGGTTGGGATTTCCAGCAAAAGCAGTGCCAGACAAGCCAGTCCCTAGAGGGATCCCATTGACGCCATTCACAGTAGGGAGAGCACCATTGGCATTTGGGATCGCTACACCACCGTCTGGGGAAGAAACCCTATAGGTGTTGCAAAGGGTACTTGACCAGTGTATATGTTCCCCAGCAGACCAGTAATCGGTCTCGCCGTTGGGCTGGCACCCCCAAGAATGTCGTGCATATAAAATTCAAGGACTGGCTCATGTCCCCCGACATTGGCAGTGACAGCTGCAGACTTAAACGCCACGAAATGCACTACGATGATTGCAGTTGCAAGGAAGGCGGGAAAAGATGATTGCCTGATGAACATGTTGCAGATTCAGGTATGAAGAACTTCTCTTTCTTGTAGAACTCAGAAGATTATAATTCTTGTGGTGATACGCAAATGAATTCAAACTCTACTCTTTATATAGTGCTGAAATTATTAGATCAATTTGGATTTTGGAGTTGGTATTAACCACCAAAAATGGTGAAGTTGTTGGATTCTGTTCTTGAAGACATGCATAGTGTTTTTGTTTCATCATATCGAAGTTATTTG

mRNA sequence

GGGTATATTTGAAAATGTACCAAATTTAATTAGATTGTTAAAATGAGCGAAGGCGATGAATCCAATTCTTGAACAGTGCGAGTAGAGAAAGACAGAGATAACATATCATCAAGCGATGAATTCGAAGCCGGATGAAGACGAACAACAGCGACGGCAATGGGGAACAAGAGCGTGGGCCACACTTGCCGCCATACGAACCCGATCGCCGCTCATCCAATGCATCACCAACTTCGTTTCAATGGATTTAATGGCCAATACTCTCTTATCCGCCGGAGCCTCGCCGGCCATGCTTCATTCCGTCGAGGAGTTGCCGGATTTCACTCCCAACGTTGACGCTCTCTGTATCAATGTCGGCACGCTTTCCCCCGCCTGGTTACCCGCCATGAAGTTCGCCGCTGAACTGGCGGTAAAGGCCGGGAAACCTTGGGTTCTCGATCCGGTGGCGGCCGGAGCATCTAAGTACCGGATGATGGCTTGTTTGGAGCTTATGAATTTGAAGCCCACCGTTGTTAGAGGGAATGGGTCAGAAATAATCGCTCTGTCTAAAGCTTCTTCGGATTCCAGTATGGGTGTGGATAGCTGCCACGACTCGGTGGATGCTGTAGAAGCTGCTAAATCATTAGCCCAATCCAGTGGAGCCATAGTAGCAGTATCAGGAGCCGTGGACATAGTTACAGACGGGAACCAAGTTATTGGTGCTCGTAATGGTGTGGCCATGATGCAAAAGATTACAGCAACTGGATGCTCTGTAACAGCTCTTATTGCTGCCTTTCTTGCTGTTGATCAACTTCATGCTTTAGAAGCAACAGCCTCGGCTTTATCAATATTTGGAATTGCAGGAGAGATGGGAATGGACGTTGCAAAGGGCCCTGCATCACTACGAACTCATTTGATTGATTCACTTTATGGGCTCGATGAAGCTTCTATAATCTCAAGAATAAGGATCTCCAGCTTGTGATTAAATTTTGGACGCGGTTTTATGTATGGTATGCCTAGTAACTAGTCTTGAACTCTTTTGAGTTTGCACAGTGCTTTAATGTTCAATAATATTGAAAAGATTGGCTACCCTACACTTTTCGTTTACATATCCATTCAAGGTTCCATTCTTTTCGGTGAAGTTATATACATCTTAATCTGATCGACCTCATAAATTTGTTTCGTTCTTTTGATGCTGTTTTTAACTATTTTTATAATCCGGTTTTATCATGAAATTCAACTAAATTCACTCATGGTATGATCACAACAGAATTACAACTGAGAAATATATAAATTTCATAAAACTGGATTACATTTTTTGACTTGCAGCTGCATTTGATAGAAACTACAGACCATGGTTGCATTCATCACCACACATATATCGCGTTGTTCTTTGTTCACCACACTGGTTTTCAGTAGGTTAGATGTACAAGGACCCTCAGCAATGTTTCTGCTCCATCTGCCACGTGCTGGCCCGGAGGAATGAGGGATCGCACCTCTGCAATCCCACGTGCATTCTTGAACTTGCCAGTTCCTCCAGTCACTGACAAATGCGATGATGGGCTACCAATCCTGAATACTCCATAGAAGTTGAGACTGTCACCATATTCTCCTCCTTCAACCATGGCTGTAAATGCCATCATCTGCGTAGTTCCATCAGCCGAACTAGCCACATATACCCCTTGTGCCTGGCCAATTGACTGTGAGCCCAACTCTGGTGCGGTGGTCAAGACGTCATCAATGACTGTAATCGTTCCAAAGCCTAGCCCCAACCCATCAGGTCCCAGTTGGGTCTGGGGGACATTTTGTGGGTTGGGATTTCCAGCAAAAGCAGTGCCAGACAAGCCAGTCCCTAGAGGGATCCCATTGACGCCATTCACAGTAGGGAGAGCACCATTGGCATTTGGGATCGCTACACCACCGTCTGGGGAAGAAACCCTATAGGTGTTGCAAAGGGTACTTGACCAGTGTATATGTTCCCCAGCAGACCAGTAATCGGTCTCGCCGTTGGGCTGGCACCCCCAAGAATGTCGTGCATATAAAATTCAAGGACTGGCTCATGTCCCCCGACATTGGCAGTGACAGCTGCAGACTTAAACGCCACGAAATGCACTACGATGATTGCAGTTGCAAGGAAGGCGGGAAAAGATGATTGCCTGATGAACATGTTGCAGATTCAGGTATGAAGAACTTCTCTTTCTTGTAGAACTCAGAAGATTATAATTCTTGTGGTGATACGCAAATGAATTCAAACTCTACTCTTTATATAGTGCTGAAATTATTAGATCAATTTGGATTTTGGAGTTGGTATTAACCACCAAAAATGGTGAAGTTGTTGGATTCTGTTCTTGAAGACATGCATAGTGTTTTTGTTTCATCATATCGAAGTTATTTG

Coding sequence (CDS)

ATGAATTCGAAGCCGGATGAAGACGAACAACAGCGACGGCAATGGGGAACAAGAGCGTGGGCCACACTTGCCGCCATACGAACCCGATCGCCGCTCATCCAATGCATCACCAACTTCGTTTCAATGGATTTAATGGCCAATACTCTCTTATCCGCCGGAGCCTCGCCGGCCATGCTTCATTCCGTCGAGGAGTTGCCGGATTTCACTCCCAACGTTGACGCTCTCTGTATCAATGTCGGCACGCTTTCCCCCGCCTGGTTACCCGCCATGAAGTTCGCCGCTGAACTGGCGGTAAAGGCCGGGAAACCTTGGGTTCTCGATCCGGTGGCGGCCGGAGCATCTAAGTACCGGATGATGGCTTGTTTGGAGCTTATGAATTTGAAGCCCACCGTTGTTAGAGGGAATGGGTCAGAAATAATCGCTCTGTCTAAAGCTTCTTCGGATTCCAGTATGGGTGTGGATAGCTGCCACGACTCGGTGGATGCTGTAGAAGCTGCTAAATCATTAGCCCAATCCAGTGGAGCCATAGTAGCAGTATCAGGAGCCGTGGACATAGTTACAGACGGGAACCAAGTTATTGGTGCTCGTAATGGTGTGGCCATGATGCAAAAGATTACAGCAACTGGATGCTCTGTAACAGCTCTTATTGCTGCCTTTCTTGCTGTTGATCAACTTCATGCTTTAGAAGCAACAGCCTCGGCTTTATCAATATTTGGAATTGCAGGAGAGATGGGAATGGACGTTGCAAAGGGCCCTGCATCACTACGAACTCATTTGATTGATTCACTTTATGGGCTCGATGAAGCTTCTATAATCTCAAGAATAAGGATCTCCAGCTTGTGA

Protein sequence

MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMACLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVSGAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL
Homology
BLAST of PI0010238 vs. ExPASy Swiss-Prot
Match: Q9LIQ4 (Hydroxyethylthiazole kinase OS=Arabidopsis thaliana OX=3702 GN=THIM PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 6.4e-98
Identity = 180/274 (65.69%), Postives = 220/274 (80.29%), Query Frame = 0

Query: 7   EDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELP 66
           E + ++ +W +  WA L A+R +SPL+QCITNFVSMDL+ANTLLSAGASPAM+HSV E+P
Sbjct: 2   ESKSEQNEWSSGVWAHLTAVRQQSPLVQCITNFVSMDLVANTLLSAGASPAMVHSVVEIP 61

Query: 67  DFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMACLELMN 126
           DFTP++ ALC+NVGTL+P WLP+MK AAELA +  KPWVLDP A   S +R+ ACLEL+ 
Sbjct: 62  DFTPHIHALCVNVGTLTPDWLPSMKAAAELASQLRKPWVLDPAAVSCSGFRLKACLELIE 121

Query: 127 LKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVSGAVDIV 186
           LKPTV++GNGSEIIALS AS   + G DS H+S DA+EAAKSLA SSGA+VAVSGAVDIV
Sbjct: 122 LKPTVIKGNGSEIIALSSASRGQTKGADSSHESTDAIEAAKSLAMSSGAVVAVSGAVDIV 181

Query: 187 TDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGM 246
           TDG QVIG  NG  MMQ+ITATGCS+  LI AFLA+D    LEAT SA+++FGIAGE+G 
Sbjct: 182 TDGKQVIGVHNGTKMMQQITATGCSLAGLIVAFLAIDSSRVLEATVSAMAVFGIAGELGE 241

Query: 247 DVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
            +A GPASLR HLID LYGLDE +++ R+ ++ L
Sbjct: 242 AMANGPASLRMHLIDCLYGLDETTVLKRVNVTRL 275

BLAST of PI0010238 vs. ExPASy Swiss-Prot
Match: K7VCB9 (Hydroxyethylthiazole kinase OS=Zea mays OX=4577 GN=THIM PE=1 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 7.1e-89
Identity = 178/275 (64.73%), Postives = 218/275 (79.27%), Query Frame = 0

Query: 7   EDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELP 66
           E+E  +  WG RAW+ L+A+R R+PL+QCITN VSMD+ AN LL+AGASPAM+HS+ E+P
Sbjct: 7   EEEMGQVWWGHRAWSLLSAVRARAPLVQCITNLVSMDIAANVLLAAGASPAMVHSLREVP 66

Query: 67  DFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMACLELMN 126
           DFTP  DA+ +NVGTLS  WLP+M+ AA     AG+PWVLDPVAA AS +RM ACLEL++
Sbjct: 67  DFTPRCDAVYVNVGTLSEDWLPSMRAAA----SAGRPWVLDPVAAAASGFRMKACLELLS 126

Query: 127 LKPTVVRGNGSEIIAL---SKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVSGAV 186
           L P VVRGN SEI+AL   S A+S +  GVDS H SVDA+EAAK+LA SS A+VAVSGAV
Sbjct: 127 LCPAVVRGNASEILALASRSTAASSNFKGVDSSHCSVDAIEAAKALALSSSAVVAVSGAV 186

Query: 187 DIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGE 246
           D VTDG QVI   NGV MMQKITATGC+VTALIAAF+A++   A+ A A AL+IFG+ GE
Sbjct: 187 DFVTDGKQVISVSNGVPMMQKITATGCAVTALIAAFVAMEPSDAIVAAACALAIFGLVGE 246

Query: 247 MGMDVAKGPASLRTHLIDSLYGLDEASIISRIRIS 279
           +GM+ AKGPASLR HLID+LY L+E ++ SR+RIS
Sbjct: 247 IGMESAKGPASLRMHLIDALYCLNEETVTSRVRIS 277

BLAST of PI0010238 vs. ExPASy Swiss-Prot
Match: B9JK65 (Hydroxyethylthiazole kinase OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-868) OX=311403 GN=thiM PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 7.9e-56
Identity = 128/264 (48.48%), Postives = 166/264 (62.88%), Query Frame = 0

Query: 17  TRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELPDFTPNVDALC 76
           T   + L A+R   PL+QCITNFV+M++ AN +L+AGASPAM+H+ EE  +F     AL 
Sbjct: 6   TTPGSLLTAMRANPPLVQCITNFVAMNIAANVMLAAGASPAMVHAEEEAGEFAAISGALT 65

Query: 77  INVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMACLELMNLKPTVVRGNG 136
           IN+GTLS  WL  M  AA+ A KAGKPWVLDPVA  A+ +R  +  +L+ L+PT++RGN 
Sbjct: 66  INIGTLSTGWLAGMTSAAQAANKAGKPWVLDPVAHYATSFRRRSVAQLLELQPTIIRGNA 125

Query: 137 SEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVSGAVDIVTDGNQVIGAR 196
           SEIIAL+  +S    GVDS      A EAA  LAQ    IVAV+GA D VTDGN+     
Sbjct: 126 SEIIALAGGTS-RGQGVDSRDPVELAEEAAVQLAQKHRTIVAVTGATDFVTDGNKASRIE 185

Query: 197 NGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGMDVAKGPASLR 256
            G  +M ++TA GCS+T L+ AF A    H L+AT +AL+ F +AGE       GP S  
Sbjct: 186 GGSPLMPQVTALGCSLTCLVGAFAATRPDHPLDATVAALAAFAVAGERAGRQVDGPGSFS 245

Query: 257 THLIDSLYGLDEASIISRIRISSL 281
              +D+L  LDE +I S  R+ SL
Sbjct: 246 WRFLDALAALDEKAIDSEARVISL 268

BLAST of PI0010238 vs. ExPASy Swiss-Prot
Match: Q1M5U3 (Hydroxyethylthiazole kinase OS=Rhizobium leguminosarum bv. viciae (strain 3841) OX=216596 GN=thiM PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.3e-55
Identity = 128/266 (48.12%), Postives = 168/266 (63.16%), Query Frame = 0

Query: 14  QWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELPDFTPNVD 73
           Q  T   A L A+R + PL+QCITN+V+M++ AN LL+AGASPAM+H+ EE  +F     
Sbjct: 2   QTRTTPGAMLKAMREKPPLVQCITNYVAMNIAANVLLAAGASPAMVHAAEEAGEFAAIAS 61

Query: 74  ALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMACLELMNLKPTVVR 133
           AL IN+GTLS  W+  M+ AA+ A  AGKPWVLDPVA  A+ +R  A  EL+ LKPT++R
Sbjct: 62  ALTINIGTLSTQWIDGMQAAAKAATSAGKPWVLDPVAHYATAFRRNAVAELLALKPTIIR 121

Query: 134 GNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVSGAVDIVTDGNQVI 193
           GN SEIIAL+   S    GVDS      A  +A+ LA+   A+VAV+GAVD VTDG + +
Sbjct: 122 GNASEIIALAGGES-RGQGVDSRDPVEQAEGSARWLAERQRAVVAVTGAVDFVTDGERAV 181

Query: 194 GARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGMDVAKGPA 253
               G A+M ++TA GCS+T L+ AF A        AT +ALS F IAGE     A GP 
Sbjct: 182 RIEGGSALMPQVTALGCSLTCLVGAFAATAPEDIFGATVAALSTFAIAGEEAALGAAGPG 241

Query: 254 SLRTHLIDSLYGLDEASIISRIRISS 280
           S     +D+L  LD  ++ +R RIS+
Sbjct: 242 SFSWRFLDALAALDAETLDARARISA 266

BLAST of PI0010238 vs. ExPASy Swiss-Prot
Match: A4WQ25 (Hydroxyethylthiazole kinase OS=Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) OX=349102 GN=thiM PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.1e-54
Identity = 124/249 (49.80%), Postives = 163/249 (65.46%), Query Frame = 0

Query: 18  RAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELPDFTPNVDALCI 77
           R  A L  +R+ +PL+QCITNFV+M++MAN LL+AGASPAM+H  EE  +F      L I
Sbjct: 3   RCGAYLDTMRSTAPLVQCITNFVAMNVMANVLLAAGASPAMVHDAEESGEFAAIAQVLTI 62

Query: 78  NVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMACLELMNLKPTVVRGNGS 137
           N+GT SP W+  M+ AA  AV AG+PWVLDPVA GA+ +R      L+ LKPTV+RGN S
Sbjct: 63  NLGTPSPRWVEGMEAAARGAVAAGRPWVLDPVAVGATAFRRGLGARLLALKPTVIRGNAS 122

Query: 138 EIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVSGAVDIVTDGNQVIGARN 197
           EI+AL+ A +    G DS      A  AA+ LA+ SGA+VAV+G VD VTDG + I   N
Sbjct: 123 EILALAGAEA-RGKGADSADTVAAAEAAAQRLAEGSGAVVAVTGPVDFVTDGRRGIRCGN 182

Query: 198 GVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGMDVAKGPASLRT 257
           G A+M K+TA GCS+T ++ AF A     A EATA+ L+ FG+AGE     A GP S + 
Sbjct: 183 GHALMPKVTALGCSLTGIVGAFAAGQP--AFEATAAGLAFFGLAGEEAARRAAGPGSFQV 242

Query: 258 HLIDSLYGL 267
             +D+L+ +
Sbjct: 243 AFLDALHAM 248

BLAST of PI0010238 vs. ExPASy TrEMBL
Match: A0A5A7U6A2 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002350 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.7e-139
Identity = 267/280 (95.36%), Postives = 275/280 (98.21%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK DEDEQQRRQWGTRAWATL+A+RT+SPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 21  MNSKHDEDEQQRRQWGTRAWATLSAVRTQSPLIQCITNFVSMDLMANTLLSAGASPAMLH 80

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           SVEELPDFTPN DALCINVGTLSPAWLPAMKFA+ELAVKAGKPWVLDPVAAGASK+RMMA
Sbjct: 81  SVEELPDFTPNADALCINVGTLSPAWLPAMKFASELAVKAGKPWVLDPVAAGASKFRMMA 140

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLELMNLKPTVVRGNGSEIIALSKAS DSSMGVDSCH+SVDAVEAAKSLA SSGAIVAVS
Sbjct: 141 CLELMNLKPTVVRGNGSEIIALSKASLDSSMGVDSCHESVDAVEAAKSLAHSSGAIVAVS 200

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG +VIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 201 GAVDIVTDGKRVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 260

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGMDVAKGPASLRTHLIDSLYGLDEA+IISRIRISSL
Sbjct: 261 AGEMGMDVAKGPASLRTHLIDSLYGLDEAAIISRIRISSL 300

BLAST of PI0010238 vs. ExPASy TrEMBL
Match: A0A0A0L6V8 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis sativus OX=3659 GN=Csa_3G133300 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 5.9e-139
Identity = 267/280 (95.36%), Postives = 273/280 (97.50%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK DEDEQQRRQWGTRAWATL+A+R RSPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 21  MNSKHDEDEQQRRQWGTRAWATLSALRARSPLIQCITNFVSMDLMANTLLSAGASPAMLH 80

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           SVEELPDFTPN DALCINVGTLSPAWLPAMKFA ELAVKAGKPWVLDPVA GASK+RMMA
Sbjct: 81  SVEELPDFTPNADALCINVGTLSPAWLPAMKFAGELAVKAGKPWVLDPVAVGASKFRMMA 140

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLELM+LKPTVVRGNGSEIIALSKAS DSSMGVDSCH+SVDAVEAAKSLAQSSGAIVAVS
Sbjct: 141 CLELMSLKPTVVRGNGSEIIALSKASLDSSMGVDSCHESVDAVEAAKSLAQSSGAIVAVS 200

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG QVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 201 GAVDIVTDGKQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 260

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGMDVAKGPASLRTHLIDSLYGLDEA+IISRIRISSL
Sbjct: 261 AGEMGMDVAKGPASLRTHLIDSLYGLDEAAIISRIRISSL 300

BLAST of PI0010238 vs. ExPASy TrEMBL
Match: A0A1S3AVZ0 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis melo OX=3656 GN=LOC103483515 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.3e-138
Identity = 267/280 (95.36%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK DEDEQQRRQWGTRAWATL+A+RTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 21  MNSKHDEDEQQRRQWGTRAWATLSAVRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 80

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           SVEELPDFTPN DALCINVGTLSPAWLPAMKFA+ELAVKAGKPWVLDPVAAGASK+RMMA
Sbjct: 81  SVEELPDFTPNADALCINVGTLSPAWLPAMKFASELAVKAGKPWVLDPVAAGASKFRMMA 140

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLELMNLKPTVVRGNGSEIIALSKAS DSSMGVDS H+SVDAVEAAKSLA SSGAIVAVS
Sbjct: 141 CLELMNLKPTVVRGNGSEIIALSKASLDSSMGVDSGHESVDAVEAAKSLAHSSGAIVAVS 200

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG +VIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 201 GAVDIVTDGKRVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 260

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGMDVAKGPASLRTHLIDSLYGLDEA+IISRIRISSL
Sbjct: 261 AGEMGMDVAKGPASLRTHLIDSLYGLDEAAIISRIRISSL 300

BLAST of PI0010238 vs. ExPASy TrEMBL
Match: A0A6J1IBK3 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucurbita maxima OX=3661 GN=LOC111473194 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.5e-131
Identity = 249/280 (88.93%), Postives = 267/280 (95.36%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK  EDE+QRR+WG R W+TLAA+R+RSPLIQCITNFVSMDL+ANTLLSAGASPAMLH
Sbjct: 1   MNSKQKEDEEQRRRWGARTWSTLAAVRSRSPLIQCITNFVSMDLVANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           S+EELPDFTPN DALCINVGTLS AWLPAMKFAAELA+KAGKPWVLDPVAAGASK+RMMA
Sbjct: 61  SIEELPDFTPNADALCINVGTLSAAWLPAMKFAAELALKAGKPWVLDPVAAGASKFRMMA 120

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLELM LKPTVVRGNGSEIIALSKAS++SSMGVDS H+S DAVEAAKSLAQ+SGAIVAVS
Sbjct: 121 CLELMQLKPTVVRGNGSEIIALSKASTESSMGVDSSHESTDAVEAAKSLAQNSGAIVAVS 180

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG QVIGA NGVAMMQKITATGCSVTALIAAFLAVDQLHALE TASALSIFGI
Sbjct: 181 GAVDIVTDGKQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALETTASALSIFGI 240

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGMD AKGPASLRTHLIDSLYGLDEA++ISR++I+SL
Sbjct: 241 AGEMGMDAAKGPASLRTHLIDSLYGLDEAAVISRVKIASL 280

BLAST of PI0010238 vs. ExPASy TrEMBL
Match: A0A6J1DUV0 (4-methyl-5-beta-hydroxyethylthiazole kinase OS=Momordica charantia OX=3673 GN=LOC111024581 PE=3 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.3e-130
Identity = 250/280 (89.29%), Postives = 268/280 (95.71%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK +EDE QRR+WG RAWATLAA+R+RSPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 1   MNSKENEDEAQRRRWGARAWATLAAVRSRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           SVEELPDFTPN DALCINVGTLS AWLPAMK AAELA KAGKPWVLDPVAAGASK+R+MA
Sbjct: 61  SVEELPDFTPNADALCINVGTLSAAWLPAMKVAAELASKAGKPWVLDPVAAGASKFRLMA 120

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLEL+NLKPTVVRGNGSEIIALSKAS++ SMGVDSCH S+DAVEAAKSLAQ+SGAIVAVS
Sbjct: 121 CLELLNLKPTVVRGNGSEIIALSKASTEPSMGVDSCHQSMDAVEAAKSLAQTSGAIVAVS 180

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG QVIGA NGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 181 GAVDIVTDGEQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGMD+AKGPASLRTHLIDSL+ LDEA+IIS+++I+SL
Sbjct: 241 AGEMGMDLAKGPASLRTHLIDSLHELDEAAIISQVKITSL 280

BLAST of PI0010238 vs. NCBI nr
Match: KAA0049185.1 (hydroxyethylthiazole kinase [Cucumis melo var. makuwa] >TYK17375.1 hydroxyethylthiazole kinase [Cucumis melo var. makuwa])

HSP 1 Score: 504.6 bits (1298), Expect = 5.5e-139
Identity = 267/280 (95.36%), Postives = 275/280 (98.21%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK DEDEQQRRQWGTRAWATL+A+RT+SPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 21  MNSKHDEDEQQRRQWGTRAWATLSAVRTQSPLIQCITNFVSMDLMANTLLSAGASPAMLH 80

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           SVEELPDFTPN DALCINVGTLSPAWLPAMKFA+ELAVKAGKPWVLDPVAAGASK+RMMA
Sbjct: 81  SVEELPDFTPNADALCINVGTLSPAWLPAMKFASELAVKAGKPWVLDPVAAGASKFRMMA 140

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLELMNLKPTVVRGNGSEIIALSKAS DSSMGVDSCH+SVDAVEAAKSLA SSGAIVAVS
Sbjct: 141 CLELMNLKPTVVRGNGSEIIALSKASLDSSMGVDSCHESVDAVEAAKSLAHSSGAIVAVS 200

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG +VIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 201 GAVDIVTDGKRVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 260

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGMDVAKGPASLRTHLIDSLYGLDEA+IISRIRISSL
Sbjct: 261 AGEMGMDVAKGPASLRTHLIDSLYGLDEAAIISRIRISSL 300

BLAST of PI0010238 vs. NCBI nr
Match: XP_004134020.2 (hydroxyethylthiazole kinase [Cucumis sativus] >XP_031738020.1 hydroxyethylthiazole kinase [Cucumis sativus] >KGN56779.1 hypothetical protein Csa_011340 [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 1.2e-138
Identity = 267/280 (95.36%), Postives = 273/280 (97.50%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK DEDEQQRRQWGTRAWATL+A+R RSPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 21  MNSKHDEDEQQRRQWGTRAWATLSALRARSPLIQCITNFVSMDLMANTLLSAGASPAMLH 80

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           SVEELPDFTPN DALCINVGTLSPAWLPAMKFA ELAVKAGKPWVLDPVA GASK+RMMA
Sbjct: 81  SVEELPDFTPNADALCINVGTLSPAWLPAMKFAGELAVKAGKPWVLDPVAVGASKFRMMA 140

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLELM+LKPTVVRGNGSEIIALSKAS DSSMGVDSCH+SVDAVEAAKSLAQSSGAIVAVS
Sbjct: 141 CLELMSLKPTVVRGNGSEIIALSKASLDSSMGVDSCHESVDAVEAAKSLAQSSGAIVAVS 200

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG QVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 201 GAVDIVTDGKQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 260

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGMDVAKGPASLRTHLIDSLYGLDEA+IISRIRISSL
Sbjct: 261 AGEMGMDVAKGPASLRTHLIDSLYGLDEAAIISRIRISSL 300

BLAST of PI0010238 vs. NCBI nr
Match: XP_008438408.1 (PREDICTED: hydroxyethylthiazole kinase [Cucumis melo])

HSP 1 Score: 501.5 bits (1290), Expect = 4.7e-138
Identity = 267/280 (95.36%), Postives = 274/280 (97.86%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK DEDEQQRRQWGTRAWATL+A+RTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH
Sbjct: 21  MNSKHDEDEQQRRQWGTRAWATLSAVRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 80

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           SVEELPDFTPN DALCINVGTLSPAWLPAMKFA+ELAVKAGKPWVLDPVAAGASK+RMMA
Sbjct: 81  SVEELPDFTPNADALCINVGTLSPAWLPAMKFASELAVKAGKPWVLDPVAAGASKFRMMA 140

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLELMNLKPTVVRGNGSEIIALSKAS DSSMGVDS H+SVDAVEAAKSLA SSGAIVAVS
Sbjct: 141 CLELMNLKPTVVRGNGSEIIALSKASLDSSMGVDSGHESVDAVEAAKSLAHSSGAIVAVS 200

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG +VIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI
Sbjct: 201 GAVDIVTDGKRVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 260

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGMDVAKGPASLRTHLIDSLYGLDEA+IISRIRISSL
Sbjct: 261 AGEMGMDVAKGPASLRTHLIDSLYGLDEAAIISRIRISSL 300

BLAST of PI0010238 vs. NCBI nr
Match: XP_038882134.1 (hydroxyethylthiazole kinase [Benincasa hispida])

HSP 1 Score: 494.6 bits (1272), Expect = 5.7e-136
Identity = 259/280 (92.50%), Postives = 271/280 (96.79%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK +EDE+QRRQWG RAWATLA +RTRSPLIQCITNFVSMDL+ANTLLSAGASPAMLH
Sbjct: 1   MNSKQNEDEEQRRQWGARAWATLATVRTRSPLIQCITNFVSMDLVANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           SVEELPDFTPN DALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVA GASK+RMMA
Sbjct: 61  SVEELPDFTPNADALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAVGASKFRMMA 120

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLELMNLKPTVVRGNGSEIIALSKAS++SSMGVDSCH+SVDAVE AKSLAQSSGAIVAVS
Sbjct: 121 CLELMNLKPTVVRGNGSEIIALSKASAESSMGVDSCHESVDAVEVAKSLAQSSGAIVAVS 180

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG QVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEAT  ALSIFGI
Sbjct: 181 GAVDIVTDGKQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATTLALSIFGI 240

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGMD+AKGPASLRTHLIDSLYGLDEA++ISR+RISSL
Sbjct: 241 AGEMGMDMAKGPASLRTHLIDSLYGLDEAALISRVRISSL 280

BLAST of PI0010238 vs. NCBI nr
Match: KAG6597318.1 (Hydroxyethylthiazole kinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028785.1 Hydroxyethylthiazole kinase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 478.0 bits (1229), Expect = 5.5e-131
Identity = 250/280 (89.29%), Postives = 268/280 (95.71%), Query Frame = 0

Query: 1   MNSKPDEDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLH 60
           MNSK  EDE+QRRQWG R W+TLAA+R+RSPLIQCITNFVSMDL+ANTLLSAGASPAMLH
Sbjct: 1   MNSKQIEDEEQRRQWGARTWSTLAAVRSRSPLIQCITNFVSMDLVANTLLSAGASPAMLH 60

Query: 61  SVEELPDFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMA 120
           S+EELPDFTPNVDALCINVGTLS AWLPAMKFAAELA+KAGKPWVLDPVAAGASK+RMMA
Sbjct: 61  SIEELPDFTPNVDALCINVGTLSAAWLPAMKFAAELALKAGKPWVLDPVAAGASKFRMMA 120

Query: 121 CLELMNLKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVS 180
           CLELM LKPTVVRGNGSEIIALSKAS++SSMGVDS H+S DAVEAAKSLAQ+SGAIVAVS
Sbjct: 121 CLELMKLKPTVVRGNGSEIIALSKASTESSMGVDSSHESTDAVEAAKSLAQTSGAIVAVS 180

Query: 181 GAVDIVTDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGI 240
           GAVDIVTDG QVIGA NGVAMMQKITATGCSVTALIAAFLAVDQLHALE TASALSIFGI
Sbjct: 181 GAVDIVTDGKQVIGAHNGVAMMQKITATGCSVTALIAAFLAVDQLHALETTASALSIFGI 240

Query: 241 AGEMGMDVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
           AGEMGM+ AKGPASLRTHLIDSLYGLDEA++ISR++I+SL
Sbjct: 241 AGEMGMETAKGPASLRTHLIDSLYGLDEAAVISRVKIASL 280

BLAST of PI0010238 vs. TAIR 10
Match: AT3G24030.1 (hydroxyethylthiazole kinase family protein )

HSP 1 Score: 358.6 bits (919), Expect = 4.5e-99
Identity = 180/274 (65.69%), Postives = 220/274 (80.29%), Query Frame = 0

Query: 7   EDEQQRRQWGTRAWATLAAIRTRSPLIQCITNFVSMDLMANTLLSAGASPAMLHSVEELP 66
           E + ++ +W +  WA L A+R +SPL+QCITNFVSMDL+ANTLLSAGASPAM+HSV E+P
Sbjct: 2   ESKSEQNEWSSGVWAHLTAVRQQSPLVQCITNFVSMDLVANTLLSAGASPAMVHSVVEIP 61

Query: 67  DFTPNVDALCINVGTLSPAWLPAMKFAAELAVKAGKPWVLDPVAAGASKYRMMACLELMN 126
           DFTP++ ALC+NVGTL+P WLP+MK AAELA +  KPWVLDP A   S +R+ ACLEL+ 
Sbjct: 62  DFTPHIHALCVNVGTLTPDWLPSMKAAAELASQLRKPWVLDPAAVSCSGFRLKACLELIE 121

Query: 127 LKPTVVRGNGSEIIALSKASSDSSMGVDSCHDSVDAVEAAKSLAQSSGAIVAVSGAVDIV 186
           LKPTV++GNGSEIIALS AS   + G DS H+S DA+EAAKSLA SSGA+VAVSGAVDIV
Sbjct: 122 LKPTVIKGNGSEIIALSSASRGQTKGADSSHESTDAIEAAKSLAMSSGAVVAVSGAVDIV 181

Query: 187 TDGNQVIGARNGVAMMQKITATGCSVTALIAAFLAVDQLHALEATASALSIFGIAGEMGM 246
           TDG QVIG  NG  MMQ+ITATGCS+  LI AFLA+D    LEAT SA+++FGIAGE+G 
Sbjct: 182 TDGKQVIGVHNGTKMMQQITATGCSLAGLIVAFLAIDSSRVLEATVSAMAVFGIAGELGE 241

Query: 247 DVAKGPASLRTHLIDSLYGLDEASIISRIRISSL 281
            +A GPASLR HLID LYGLDE +++ R+ ++ L
Sbjct: 242 AMANGPASLRMHLIDCLYGLDETTVLKRVNVTRL 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIQ46.4e-9865.69Hydroxyethylthiazole kinase OS=Arabidopsis thaliana OX=3702 GN=THIM PE=1 SV=1[more]
K7VCB97.1e-8964.73Hydroxyethylthiazole kinase OS=Zea mays OX=4577 GN=THIM PE=1 SV=1[more]
B9JK657.9e-5648.48Hydroxyethylthiazole kinase OS=Agrobacterium radiobacter (strain K84 / ATCC BAA-... [more]
Q1M5U32.3e-5548.12Hydroxyethylthiazole kinase OS=Rhizobium leguminosarum bv. viciae (strain 3841) ... [more]
A4WQ251.1e-5449.80Hydroxyethylthiazole kinase OS=Rhodobacter sphaeroides (strain ATCC 17025 / ATH ... [more]
Match NameE-valueIdentityDescription
A0A5A7U6A22.7e-13995.364-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A0A0L6V85.9e-13995.364-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A1S3AVZ02.3e-13895.364-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucumis melo OX=3656 GN=LOC103483... [more]
A0A6J1IBK34.5e-13188.934-methyl-5-beta-hydroxyethylthiazole kinase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1DUV02.3e-13089.294-methyl-5-beta-hydroxyethylthiazole kinase OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
KAA0049185.15.5e-13995.36hydroxyethylthiazole kinase [Cucumis melo var. makuwa] >TYK17375.1 hydroxyethylt... [more]
XP_004134020.21.2e-13895.36hydroxyethylthiazole kinase [Cucumis sativus] >XP_031738020.1 hydroxyethylthiazo... [more]
XP_008438408.14.7e-13895.36PREDICTED: hydroxyethylthiazole kinase [Cucumis melo][more]
XP_038882134.15.7e-13692.50hydroxyethylthiazole kinase [Benincasa hispida][more]
KAG6597318.15.5e-13189.29Hydroxyethylthiazole kinase, partial [Cucurbita argyrosperma subsp. sororia] >KA... [more]
Match NameE-valueIdentityDescription
AT3G24030.14.5e-9965.69hydroxyethylthiazole kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000417Hydroxyethylthiazole kinasePRINTSPR01099HYETHTZKNASEcoord: 176..193
score: 45.06
coord: 44..65
score: 48.48
coord: 101..118
score: 55.56
coord: 31..38
score: 63.89
coord: 131..145
score: 42.22
coord: 75..83
score: 61.73
IPR000417Hydroxyethylthiazole kinasePIRSFPIRSF000513Thz_kinasecoord: 17..280
e-value: 1.2E-91
score: 304.4
IPR000417Hydroxyethylthiazole kinasePFAMPF02110HKcoord: 23..267
e-value: 6.5E-68
score: 228.8
IPR000417Hydroxyethylthiazole kinaseHAMAPMF_00228Thz_kinasecoord: 25..272
score: 29.186951
IPR000417Hydroxyethylthiazole kinaseCDDcd01170THZ_kinasecoord: 23..264
e-value: 2.79395E-106
score: 306.773
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 3..277
e-value: 5.3E-87
score: 293.6
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 16..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0010238.2PI0010238.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009228 thiamine biosynthetic process
biological_process GO:0009229 thiamine diphosphate biosynthetic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0004417 hydroxyethylthiazole kinase activity
molecular_function GO:0046872 metal ion binding