PI0009331 (gene) Melon (PI 482460) v1

Overview
NamePI0009331
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionbasic 7S globulin-like
Locationchr11: 4805060 .. 4806597 (+)
RNA-Seq ExpressionPI0009331
SyntenyPI0009331
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAAACGATATCAAATTCATTTCCACCTCAGCATTTTTCATCTTCATTAAAAAATTAAATTAAATTAAAATTTATATCCATAAATTTTATAAATATATTCTCTCAAACAAACTCCTCCACCTGGCTCTTATCCAAAAATTTTCCTCTTCCAAAGTCTTCACTCTCTCTCTCCCTCCCTCTTTAACCTATTCCCAATCCGCGGCGAACCTCCGCCGACTGTACCAGAAATTTCCATGGCTGTTCCCCATTCTCCCTTCCTTCTTCTTCTTCTTCTTCTTCTCTCTTCCTTAACCCTCTTCCTTGGCCAAACCTTCTCTCTCGTCATCCCCCTCACTAAAGATTCCCTCACCAATCAGTATTTGGCCACTGTTTTCCATGGTTCTCCGATTAAGCCCGTCCATCTCGCAGTCGATCTTGGTGGCCAGTCTCTCTGGATGGCTTGTGGTGGATCTTCTTCCAGCCGTTCGATTCCCAGCCGCTCTATTCAGTGTATTGCGGCTACTGGTGGGGGTAGATCTGGGTCTGTTGGTGGCGCGTGTGATGTAATCGCTGGGAATCCCTTTGGGGATTTGGAAGGGAAGGCTATTCTGGTCGAGGATATGGTGGCCGTGAGGTCTTTGGACCGTTCTACGGCAGCGGTGATTGTCGCTCTTCATTCTTGTGCTCCGAGATTTCTTTTACAAGGTTTGGCAAAATCTGTTAAAGGGATTTTAGGGCTTGGCCGGAATCAAATCTCGCTTCCGGCTCAGCTCGCGACGGAGCTTGGTTCTAACCGGCGGTTTTCTATTTGTCTGTCGTCGACGTACGGTGTTGTTTTTCCGGATAGTGGAAGTCAAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACTAAGATAATTGACGCCTCGCAGTCGCCGGAGTATTTTATTAATGTAAAAGCCATTAAAGTAGACGGTAACCGCCTCGATTTGAACAAGTCGTTGCTCGATTTAGAGGGAGGAGGCGACGGAGGAGGTGGAGGAGGGACGAGATTAAGTACGGTTGTGCCGTACACTGCTTTAGAGAGTTCAATCTTTAACTCTTTGACGACAGCTTTCAGGGCGGCAGCGGCAGCGATGAATATGAAAGAAGTGGCGCCGGTAGCGCCGTTTGAGGTGTGTTTTGAATCGGAAAACATGGAGATGACGGCAACAGGGCCGAAAGTGCCGGAGATTGAACTGATCTTACAGAGCGAGATGGTGGGATGGAAAATCTATGGAAGAAATTCAATGGTGAAGGTTAACGACGAAGCATTTTGTTTAGGGTTTGTCGATGGCGGATTGAAGCCAAAAAACGCCATCGTTTTGGGAGGGTATCAAATGGAGGATATCGTTCTTGATTTTGACATGGGAACTTCTATGCTCGGATTTAGCTCCTCTTTGTTACAGAGGAAGAGATCTTGCTCGGAATTTTCGCCGGAAAATTTACTGGAAAGAACCGGATGATTTGGGGAAGATAAACTTAAATTAGGGCTTAAATTTTGAGGGCTTTTTTT

mRNA sequence

TAAAAACGATATCAAATTCATTTCCACCTCAGCATTTTTCATCTTCATTAAAAAATTAAATTAAATTAAAATTTATATCCATAAATTTTATAAATATATTCTCTCAAACAAACTCCTCCACCTGGCTCTTATCCAAAAATTTTCCTCTTCCAAAGTCTTCACTCTCTCTCTCCCTCCCTCTTTAACCTATTCCCAATCCGCGGCGAACCTCCGCCGACTGTACCAGAAATTTCCATGGCTGTTCCCCATTCTCCCTTCCTTCTTCTTCTTCTTCTTCTTCTCTCTTCCTTAACCCTCTTCCTTGGCCAAACCTTCTCTCTCGTCATCCCCCTCACTAAAGATTCCCTCACCAATCAGTATTTGGCCACTGTTTTCCATGGTTCTCCGATTAAGCCCGTCCATCTCGCAGTCGATCTTGGTGGCCAGTCTCTCTGGATGGCTTGTGGTGGATCTTCTTCCAGCCGTTCGATTCCCAGCCGCTCTATTCAGTGTATTGCGGCTACTGGTGGGGGTAGATCTGGGTCTGTTGGTGGCGCGTGTGATGTAATCGCTGGGAATCCCTTTGGGGATTTGGAAGGGAAGGCTATTCTGGTCGAGGATATGGTGGCCGTGAGGTCTTTGGACCGTTCTACGGCAGCGGTGATTGTCGCTCTTCATTCTTGTGCTCCGAGATTTCTTTTACAAGGTTTGGCAAAATCTGTTAAAGGGATTTTAGGGCTTGGCCGGAATCAAATCTCGCTTCCGGCTCAGCTCGCGACGGAGCTTGGTTCTAACCGGCGGTTTTCTATTTGTCTGTCGTCGACGTACGGTGTTGTTTTTCCGGATAGTGGAAGTCAAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACTAAGATAATTGACGCCTCGCAGTCGCCGGAGTATTTTATTAATGTAAAAGCCATTAAAGTAGACGGTAACCGCCTCGATTTGAACAAGTCGTTGCTCGATTTAGAGGGAGGAGGCGACGGAGGAGGTGGAGGAGGGACGAGATTAAGTACGGTTGTGCCGTACACTGCTTTAGAGAGTTCAATCTTTAACTCTTTGACGACAGCTTTCAGGGCGGCAGCGGCAGCGATGAATATGAAAGAAGTGGCGCCGGTAGCGCCGTTTGAGGTGTGTTTTGAATCGGAAAACATGGAGATGACGGCAACAGGGCCGAAAGTGCCGGAGATTGAACTGATCTTACAGAGCGAGATGGTGGGATGGAAAATCTATGGAAGAAATTCAATGGTGAAGGTTAACGACGAAGCATTTTGTTTAGGGTTTGTCGATGGCGGATTGAAGCCAAAAAACGCCATCGTTTTGGGAGGGTATCAAATGGAGGATATCGTTCTTGATTTTGACATGGGAACTTCTATGCTCGGATTTAGCTCCTCTTTGTTACAGAGGAAGAGATCTTGCTCGGAATTTTCGCCGGAAAATTTACTGGAAAGAACCGGATGATTTGGGGAAGATAAACTTAAATTAGGGCTTAAATTTTGAGGGCTTTTTTT

Coding sequence (CDS)

ATGGCTGTTCCCCATTCTCCCTTCCTTCTTCTTCTTCTTCTTCTTCTCTCTTCCTTAACCCTCTTCCTTGGCCAAACCTTCTCTCTCGTCATCCCCCTCACTAAAGATTCCCTCACCAATCAGTATTTGGCCACTGTTTTCCATGGTTCTCCGATTAAGCCCGTCCATCTCGCAGTCGATCTTGGTGGCCAGTCTCTCTGGATGGCTTGTGGTGGATCTTCTTCCAGCCGTTCGATTCCCAGCCGCTCTATTCAGTGTATTGCGGCTACTGGTGGGGGTAGATCTGGGTCTGTTGGTGGCGCGTGTGATGTAATCGCTGGGAATCCCTTTGGGGATTTGGAAGGGAAGGCTATTCTGGTCGAGGATATGGTGGCCGTGAGGTCTTTGGACCGTTCTACGGCAGCGGTGATTGTCGCTCTTCATTCTTGTGCTCCGAGATTTCTTTTACAAGGTTTGGCAAAATCTGTTAAAGGGATTTTAGGGCTTGGCCGGAATCAAATCTCGCTTCCGGCTCAGCTCGCGACGGAGCTTGGTTCTAACCGGCGGTTTTCTATTTGTCTGTCGTCGACGTACGGTGTTGTTTTTCCGGATAGTGGAAGTCAAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACTAAGATAATTGACGCCTCGCAGTCGCCGGAGTATTTTATTAATGTAAAAGCCATTAAAGTAGACGGTAACCGCCTCGATTTGAACAAGTCGTTGCTCGATTTAGAGGGAGGAGGCGACGGAGGAGGTGGAGGAGGGACGAGATTAAGTACGGTTGTGCCGTACACTGCTTTAGAGAGTTCAATCTTTAACTCTTTGACGACAGCTTTCAGGGCGGCAGCGGCAGCGATGAATATGAAAGAAGTGGCGCCGGTAGCGCCGTTTGAGGTGTGTTTTGAATCGGAAAACATGGAGATGACGGCAACAGGGCCGAAAGTGCCGGAGATTGAACTGATCTTACAGAGCGAGATGGTGGGATGGAAAATCTATGGAAGAAATTCAATGGTGAAGGTTAACGACGAAGCATTTTGTTTAGGGTTTGTCGATGGCGGATTGAAGCCAAAAAACGCCATCGTTTTGGGAGGGTATCAAATGGAGGATATCGTTCTTGATTTTGACATGGGAACTTCTATGCTCGGATTTAGCTCCTCTTTGTTACAGAGGAAGAGATCTTGCTCGGAATTTTCGCCGGAAAATTTACTGGAAAGAACCGGATGA

Protein sequence

MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILVEDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSNRRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKVDGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKEVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG
Homology
BLAST of PI0009331 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.7e-70
Identity = 172/430 (40.00%), Postives = 247/430 (57.44%), Query Frame = 0

Query: 9   LLLLLLLLSSLTLFLGQTF---SLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGGQS 68
           LL  LL ++S T     +F    L++P+TKD+LT QYL  +   +P+ PV L +DLGGQ 
Sbjct: 11  LLCSLLFITSTTAQSETSFRPKGLILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQF 70

Query: 69  LWMACGG---SSSSRSIPSRSIQC-IAATGGG--------RSGSVGGACDVIAGNPFGDL 128
           LW+ C     SS+ R    RS QC +A  G G        + G     C ++  N     
Sbjct: 71  LWVDCDQGYVSSTYRPARCRSAQCSLAGAGSGCGQCFSPPKPGCNNNTCGLLPDNTITRT 130

Query: 129 EGKAILVEDMVAVRSLDRSTAAVIVA----LHSCAPRFLLQGLAKSVKGILGLGRNQISL 188
                L  D V V+S +       V+    L  C   FLL+GLA  VKG+ GLGR +ISL
Sbjct: 131 ATSGELASDTVQVQSSNGKNPGRHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISL 190

Query: 189 PAQLATELGSNRRFSICLSS---TYGVVFPDSGSQDSVYGSEISSS-LTYTPILTKIIDA 248
           P+Q + E    R+F++CLSS   + GVV    G    +   E +++  +YTP+    +  
Sbjct: 191 PSQFSAEFSFPRKFAVCLSSSTNSKGVVLFGDGPYTFLPNREFANNDFSYTPLFINPVST 250

Query: 249 SQ-------SPEYFINVKAIKVDGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALE 308
           +        S EYFI VK+IK++   + +N +LL +    D  G GGT++STV PYT LE
Sbjct: 251 ASAFSSREPSSEYFIGVKSIKINEKVVPINTTLLSI----DNQGVGGTKISTVNPYTILE 310

Query: 309 SSIFNSLTTAFRAAAAAMNMKEVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVG 368
           +SI+N++T  F      +N+  VA VAPF  CF+S N+  T  GP VP I+L+LQ+E V 
Sbjct: 311 TSIYNAVTNFF--VKELVNITRVASVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVF 370

Query: 369 WKIYGRNSMVKVNDEAFCLGFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLL 409
           W+I+G NSMV+V++   CLGFVDGG+ P+ +IV+GGY +ED +L FD+  S LGF+SS+L
Sbjct: 371 WRIFGANSMVQVSENVLCLGFVDGGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSIL 430

BLAST of PI0009331 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 230.3 bits (586), Expect = 3.9e-59
Identity = 152/416 (36.54%), Postives = 231/416 (55.53%), Query Frame = 0

Query: 16  LSSLTLFLGQTFS--LVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGGQSLWMACGG- 75
           L+ L LFL  TF   L+ P++KD  T  Y  +VF  +P++P  L + LG    W+ C   
Sbjct: 13  LAILFLFLTPTFQIPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLCDST 72

Query: 76  --SSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILVEDMVAVRSLD 135
             SSSS  IP  +  C +      S +    C +   NP          + D +A+ + D
Sbjct: 73  YTSSSSHHIPCNTPLCNSFPSNACSNN-SSLCALFPENPVTRNTLLDTALIDSLALPTYD 132

Query: 136 RSTAAVIVA--LHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSNRRFSICL- 195
            S++ V+++  + SCA   LLQGLA +  G+  LGR+  SLPAQ++T L S R F++CL 
Sbjct: 133 ASSSLVLISDFIFSCATAHLLQGLAANALGLASLGRSNYSLPAQISTSLTSPRSFTLCLP 192

Query: 196 --SSTYGVVFPDSGSQDSVYGSEISSSLTYTPIL------TKIIDASQ-SPEYFINVKAI 255
             S+  G     S +   ++ S+I   LTYT ++      T + D  Q S EYFIN+ +I
Sbjct: 193 ASSANTGAAIFASTASSFLFSSKI--DLTYTQLIVNPVADTVVTDNPQPSDEYFINLTSI 252

Query: 256 KVDGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNM 315
           K++G  L +N S+L +    D  G GGT++ST  PYT LE+SI+      F   ++A N+
Sbjct: 253 KINGKPLYINSSILTV----DQTGFGGTKISTAEPYTVLETSIYRLFVQRFVNESSAFNL 312

Query: 316 KEVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKV---NDEAF 375
                V PF VC+ + ++  T  GP VP ++L++ SE V W+I+G NSMV+V     + +
Sbjct: 313 TVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHSEDVFWRIFGGNSMVRVAKGGVDVW 372

Query: 376 CLGFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPEN 412
           CLGFVDGG + +  IV+GG+Q+ED ++ FD+ ++  GF+S+LL +   CS     N
Sbjct: 373 CLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRFGFTSTLLLQDAKCSNLKVNN 421

BLAST of PI0009331 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 5.7e-50
Identity = 146/426 (34.27%), Postives = 218/426 (51.17%), Query Frame = 0

Query: 10  LLLLLLLSSLTLFLGQTF------SLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGG 69
           L L++L SS T    QT        LV+ + KD  TN ++  +   +P+      +DL G
Sbjct: 15  LSLIILTSSAT--KSQTHVPIRPNKLVLKVQKDRATNLHVVQIHKRTPLVQFPFVIDLTG 74

Query: 70  QSLWMACGG---SSSSRSIPSRSIQCIAA---------TGGGRSGSVGGACDVIAGNPFG 129
           + L + C     SS+ ++    S QC  A         +   R G    AC ++  NP  
Sbjct: 75  RFLSVNCENQYTSSTYKAPVCHSSQCARANSHTCRTCSSSKTRPGCHTNACGLLTTNPVT 134

Query: 130 DLEGKAILVEDMVAVRSLDRSTAAVIVA----LHSCAPRFLLQ-GLAKSVKGILGLGRNQ 189
               +  L ED++ + S   S+   +V     L +CAP  +LQ GL K+V+G+ GLG + 
Sbjct: 135 QQSAQGELAEDVLKIPSTQGSSPGPMVTYPHFLFACAPSNILQKGLPKNVQGVAGLGHSP 194

Query: 190 ISLPAQLATELGSNRRFSICLSST---YGVVFPDSGSQDSVYGSEISSSLTYTPILTKII 249
           ISLP QLA+  G   +F++CL+S+    G VF   G      G ++S  LTY P      
Sbjct: 195 ISLPYQLASHFGFPPKFAVCLTSSPGKNGAVFFGEGPYFMKPGIDVSRQLTYAP-----F 254

Query: 250 DASQSPEYFINVKAIKVDGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFN 309
              Q  EY+INV++ K++   L               GG GG  +ST  PYT L++ IF 
Sbjct: 255 TIGQQGEYYINVQSFKINNAMLPSIPK----------GGFGGAMISTTTPYTTLQTPIFR 314

Query: 310 SLTTAFRAAAAAMNMKEVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEM-VGWKIY 369
           +L   F        +  V PVAPF  CF++  +  +  GP VP I+L+L ++  + W+I+
Sbjct: 315 ALNQLF--MNQLRGVPHVKPVAPFGACFDANRIPTSKMGPTVPSIDLVLDNKKNIMWRIF 374

Query: 370 GRNSMVKVNDEAFCLGFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKR 409
           G N+M++      CL FVDGG++PK  IV+G  Q+ED +L FD+  S LGFSSSLL R+ 
Sbjct: 375 GANAMIQPRPGVMCLAFVDGGMRPKAPIVIGTQQLEDNLLQFDLMNSRLGFSSSLLFRRT 421

BLAST of PI0009331 vs. ExPASy Swiss-Prot
Match: Q9FSH9 (Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 4.3e-42
Identity = 132/409 (32.27%), Postives = 203/409 (49.63%), Query Frame = 0

Query: 29  LVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGGQSLWMACGG--SSSSRSIP------ 88
           LV+P+ +D+ T  +   +   +P+  V + +DL G+ LW+ C    SSS+   P      
Sbjct: 48  LVLPIQQDASTKLHWGNILKRTPLMQVPVLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQ 107

Query: 89  ---SRSIQCIAATGG--GRSGSVGGACDVIAGNPFGDLEGKAILVEDMVAVRSLDRSTAA 148
              + + QC   T     R G     C +I+ NP     G   L +D++A+ S   S   
Sbjct: 108 CSRANTHQCFTCTDSTTSRPGCHNNTCGLISSNPVTQESGLGELAQDVLALHSTHGSKLG 167

Query: 149 VIVA----LHSCAPRFLLQ-GLAKSVKGILGLGRNQISLPAQLATELGSNRRFSICLSS- 208
            +V     L SCAP FL Q GL  +V+G LGLG   ISLP QL +  G  R+F++CLSS 
Sbjct: 168 SLVKIPQFLFSCAPTFLTQKGLPNNVQGALGLGHAPISLPNQLFSHFGLKRQFTMCLSSY 227

Query: 209 --TYGVV----FPDSGSQDSVYGS-EISSSLTYTPILTKIIDASQSPEYFINVKAIKVDG 268
             + G +      D  + + ++ S ++   + YTP     +  S+  EYFI V AI+V+ 
Sbjct: 228 PTSNGAILFGDINDPNNNNYIHNSLDVLHDMVYTP-----LTISKQGEYFIQVSAIRVNK 287

Query: 269 NRL--DLNKSLLDLEGGG---DGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMN 328
           + +    N S+          +    GG  ++T  PYT L  SIF   T  F  A     
Sbjct: 288 HMVIPTKNPSMFPSSSSSSYHESSEIGGAMITTTNPYTVLRHSIFEVFTQVF--ANNVPK 347

Query: 329 MKEVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCL 388
             +V  V PF +C++++ +        VP ++LI+    V W+I G N MV+  D   CL
Sbjct: 348 QAQVKAVGPFGLCYDTKKI-----SGGVPSVDLIMDKSDVVWRISGENLMVQAQDGVSCL 407

Query: 389 GFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKRSCS 406
           GFVDGG+  +  I LG +Q+E+ ++ FD+  S +GF ++SL    +SCS
Sbjct: 408 GFVDGGVHTRAGIALGTHQLEENLVVFDLARSRVGFNTNSLKSHGKSCS 444

BLAST of PI0009331 vs. ExPASy Swiss-Prot
Match: Q42369 (Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.1e-40
Identity = 134/407 (32.92%), Postives = 201/407 (49.39%), Query Frame = 0

Query: 29  LVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGGQSLWMACGG--SSSSRSIP------ 88
           LV+P+ +D+ T  + A +   +P+  V L +DL G+ LW+ C    SSS+   P      
Sbjct: 47  LVLPVQEDASTGLHWANIHKRTPLMQVPLLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQ 106

Query: 89  ---SRSIQCIAATGG--GRSGSVGGACDVIAGNPFGDLEGKAILVEDMVAVRSLDRSTAA 148
              + + QC   T     R G     C +++ NP     G   L +D++A+ S   S   
Sbjct: 107 CSRANTHQCFTCTDSTTTRPGCHNNTCGLLSSNPVTQESGLGELAQDVLAIHSTHGSKLG 166

Query: 149 VIVA----LHSCAPRFLLQ-GLAKSVKGILGLGRNQISLPAQLATELGSNRRFSICLS-- 208
            +V     L SCAP FL Q GL  +V+G LGLG+  ISL  QL +  G  R+FS+CLS  
Sbjct: 167 PMVKVPQFLFSCAPSFLAQKGLPNNVQGALGLGQAPISLQNQLFSHFGLKRQFSVCLSRY 226

Query: 209 --STYGVVF---PDSGSQDSVYGS-EISSSLTYTPILTKIIDASQSPEYFINVKAIKVDG 268
             S   ++F    D  + + ++ S ++   L YTP     +  S+  EYFI V AI+V+ 
Sbjct: 227 STSNGAILFGDINDPNNNNYIHNSLDVLHDLVYTP-----LTISKQGEYFIQVNAIRVNK 286

Query: 269 NRLDLNKS-LLDLEGGGDGGGG--GGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMK 328
           + +   K+  +        G G  GG  ++T  PYT L  SIF   T  F  A       
Sbjct: 287 HLVIPTKNPFISPSSTSYHGSGEIGGALITTTHPYTVLSHSIFEVFTQVF--ANNMPKQA 346

Query: 329 EVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGF 388
           +V  V PF +C++S  +   A     P ++LIL      W+I   N MV+  D   CLGF
Sbjct: 347 QVKAVGPFGLCYDSRKISGGA-----PSVDLILDKNDAVWRISSENFMVQAQDGVSCLGF 406

Query: 389 VDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKRSCS 406
           VDGG+  +  I LG + +E+ ++ FD+  S +GF S+SL    ++CS
Sbjct: 407 VDGGVHARAGIALGAHHLEENLVVFDLERSRVGFNSNSLKSYGKTCS 441

BLAST of PI0009331 vs. ExPASy TrEMBL
Match: A0A0A0KHJ8 (Basic 7S globulin 2 small subunit OS=Cucumis sativus OX=3659 GN=Csa_6G237600 PE=4 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 9.2e-213
Identity = 394/417 (94.48%), Postives = 401/417 (96.16%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MAVPH PF   LLLLLSSLTLF GQTFSLVIPLTKDSLTNQY+ATVFHGSPIKPVHLAVD
Sbjct: 1   MAVPHFPF---LLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYVATVFHGSPIKPVHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV
Sbjct: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKG+LGLGRNQISLPAQ+ATELGS+
Sbjct: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELGSH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFS+CLSST GVVFPDSGSQDSVYGSEISSSLTYTPILTK IDA QSPEYFINVKAIKV
Sbjct: 181 RRFSLCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDALQSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDLEG GDG GGGGTRLSTVVPYT LESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLEGVGDGEGGGGTRLSTVVPYTVLESSIFNSLTAAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVAPFEVCFESENMEMTA GPKVPEIELILQSEMVGWKIYGRNSMVKVNDEA+CLGFV
Sbjct: 301 VAPVAPFEVCFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAYCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSP NLLE TG
Sbjct: 361 DGGLKPRNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPVNLLESTG 414

BLAST of PI0009331 vs. ExPASy TrEMBL
Match: A0A5A7SQG3 (Basic 7S globulin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00740 PE=4 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 6.6e-211
Identity = 390/417 (93.53%), Postives = 401/417 (96.16%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MAVPHSPFLLLLLLLLSSLTLF GQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD
Sbjct: 1   MAVPHSPFLLLLLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGG+SLWMACGGSSSSRSIPSRSIQCIAAT GGRSGSVGGAC+VIA NPFGDLEGKAILV
Sbjct: 61  LGGRSLWMACGGSSSSRSIPSRSIQCIAATAGGRSGSVGGACNVIAENPFGDLEGKAILV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VAV SL RSTAAVIVALHSCAPRFLLQGLAKSVKG+LGLGRNQISLPAQ+ATELGS+
Sbjct: 121 EDTVAVGSLGRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELGSH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFS+CLSST GVVFPDSGS DSVYGSEISSSLTYTPILTK IDA  SPEYFINVKAIKV
Sbjct: 181 RRFSLCLSSTNGVVFPDSGSPDSVYGSEISSSLTYTPILTKKIDALLSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDLE  GDGGGGGGTRLSTVVPYTALESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLEEAGDGGGGGGTRLSTVVPYTALESSIFNSLTVAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVAPFE CFESENMEMTA GPKVPEIELILQSEMVGWKIYGRNSMVKVND+A+CLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDKAYCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NAIVLGGYQMEDIVLDFD+GTSMLGFSSSLLQRKRSCSEFSPENLL+RTG
Sbjct: 361 DGGLKPRNAIVLGGYQMEDIVLDFDIGTSMLGFSSSLLQRKRSCSEFSPENLLKRTG 417

BLAST of PI0009331 vs. ExPASy TrEMBL
Match: A0A1S3C7N9 (basic 7S globulin-like OS=Cucumis melo OX=3656 GN=LOC103497928 PE=4 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 6.6e-211
Identity = 390/417 (93.53%), Postives = 401/417 (96.16%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MAVPHSPFLLLLLLLLSSLTLF GQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD
Sbjct: 1   MAVPHSPFLLLLLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGG+SLWMACGGSSSSRSIPSRSIQCIAAT GGRSGSVGGAC+VIA NPFGDLEGKAILV
Sbjct: 61  LGGRSLWMACGGSSSSRSIPSRSIQCIAATAGGRSGSVGGACNVIAENPFGDLEGKAILV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VAV SL RSTAAVIVALHSCAPRFLLQGLAKSVKG+LGLGRNQISLPAQ+ATELGS+
Sbjct: 121 EDTVAVGSLGRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELGSH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFS+CLSST GVVFPDSGS DSVYGSEISSSLTYTPILTK IDA  SPEYFINVKAIKV
Sbjct: 181 RRFSLCLSSTNGVVFPDSGSPDSVYGSEISSSLTYTPILTKKIDALLSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDLE  GDGGGGGGTRLSTVVPYTALESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLEEAGDGGGGGGTRLSTVVPYTALESSIFNSLTVAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVAPFE CFESENMEMTA GPKVPEIELILQSEMVGWKIYGRNSMVKVND+A+CLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDKAYCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NAIVLGGYQMEDIVLDFD+GTSMLGFSSSLLQRKRSCSEFSPENLL+RTG
Sbjct: 361 DGGLKPRNAIVLGGYQMEDIVLDFDIGTSMLGFSSSLLQRKRSCSEFSPENLLKRTG 417

BLAST of PI0009331 vs. ExPASy TrEMBL
Match: A0A6J1H4R8 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460386 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.1e-194
Identity = 365/417 (87.53%), Postives = 379/417 (90.89%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MAVP S  LLLL LLL    LF G  FSLVIP+ KDS+TNQYLAT++HGSPIKPVHLAVD
Sbjct: 1   MAVPESRLLLLLRLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGGQ LWMACGGSSSSR I SRSIQCIAA GGGRSGS G ACDVIAGNPFGDLEGKA+LV
Sbjct: 61  LGGQFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VAVRSLDRSTAAVIVALHSCAPRFLLQGLA+S KG+LGLGRNQISLPAQ+ATELG +
Sbjct: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAESAKGVLGLGRNQISLPAQIATELGFH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFS+CLSST GVVFPDSG+ DSVYGSEISSSLTYTPILTK IDASQSPEYFINVKAIKV
Sbjct: 181 RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDL    DGG GGGTRLSTVVPYT LESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDL----DGGSGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVAPFE CFESENMEMTATGPKVPEIELILQSEMVGWKI GRNSMVKVNDE  CLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NA+VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRK+SCSEFSPENL  R G
Sbjct: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 413

BLAST of PI0009331 vs. ExPASy TrEMBL
Match: A0A6J1L1S1 (basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111499071 PE=4 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 8.1e-193
Identity = 363/417 (87.05%), Postives = 379/417 (90.89%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MAVP S FLLLLLL  S+  LF G  FSLVIP+ KDS+TNQYLAT++HGS IKPVHLAVD
Sbjct: 1   MAVPKSRFLLLLLLCFSA--LFHGGAFSLVIPVNKDSVTNQYLATLYHGSQIKPVHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGG+ LWMACGGSSSSR I SRSIQCIAA GGGRSGS G ACDVIAGNPFGDLEGKA+LV
Sbjct: 61  LGGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VAVRSLDRSTAAVIVALHSCAPRFLLQGLAKS KG+LGLGRNQISLPAQ+ATELG +
Sbjct: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFS+CLSST GVVFPD+G+ DSVYGSEISSSLTYTPILTK  DASQSPEYFINVKAIKV
Sbjct: 181 RRFSVCLSSTNGVVFPDTGNLDSVYGSEISSSLTYTPILTKKTDASQSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDL    DGGGGGGTRLSTVVPYT L SSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDL----DGGGGGGTRLSTVVPYTTLGSSIFNSLTAAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVAPFE CFESENMEMTATGPKVPEIELILQSEMVGWKI GRNSMVKVNDE  CLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NA+VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRK+SCSEFSPENL  R G
Sbjct: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 411

BLAST of PI0009331 vs. NCBI nr
Match: XP_004151001.1 (probable aspartic proteinase GIP2 [Cucumis sativus] >KGN47256.1 hypothetical protein Csa_017018 [Cucumis sativus])

HSP 1 Score: 749.2 bits (1933), Expect = 1.9e-212
Identity = 394/417 (94.48%), Postives = 401/417 (96.16%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MAVPH PF   LLLLLSSLTLF GQTFSLVIPLTKDSLTNQY+ATVFHGSPIKPVHLAVD
Sbjct: 1   MAVPHFPF---LLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYVATVFHGSPIKPVHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV
Sbjct: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKG+LGLGRNQISLPAQ+ATELGS+
Sbjct: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELGSH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFS+CLSST GVVFPDSGSQDSVYGSEISSSLTYTPILTK IDA QSPEYFINVKAIKV
Sbjct: 181 RRFSLCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDALQSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDLEG GDG GGGGTRLSTVVPYT LESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLEGVGDGEGGGGTRLSTVVPYTVLESSIFNSLTAAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVAPFEVCFESENMEMTA GPKVPEIELILQSEMVGWKIYGRNSMVKVNDEA+CLGFV
Sbjct: 301 VAPVAPFEVCFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAYCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSP NLLE TG
Sbjct: 361 DGGLKPRNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPVNLLESTG 414

BLAST of PI0009331 vs. NCBI nr
Match: XP_008458562.1 (PREDICTED: basic 7S globulin-like [Cucumis melo] >KAA0033422.1 basic 7S globulin-like [Cucumis melo var. makuwa])

HSP 1 Score: 743.0 bits (1917), Expect = 1.4e-210
Identity = 390/417 (93.53%), Postives = 401/417 (96.16%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MAVPHSPFLLLLLLLLSSLTLF GQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD
Sbjct: 1   MAVPHSPFLLLLLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGG+SLWMACGGSSSSRSIPSRSIQCIAAT GGRSGSVGGAC+VIA NPFGDLEGKAILV
Sbjct: 61  LGGRSLWMACGGSSSSRSIPSRSIQCIAATAGGRSGSVGGACNVIAENPFGDLEGKAILV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VAV SL RSTAAVIVALHSCAPRFLLQGLAKSVKG+LGLGRNQISLPAQ+ATELGS+
Sbjct: 121 EDTVAVGSLGRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELGSH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFS+CLSST GVVFPDSGS DSVYGSEISSSLTYTPILTK IDA  SPEYFINVKAIKV
Sbjct: 181 RRFSLCLSSTNGVVFPDSGSPDSVYGSEISSSLTYTPILTKKIDALLSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDLE  GDGGGGGGTRLSTVVPYTALESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLEEAGDGGGGGGTRLSTVVPYTALESSIFNSLTVAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVAPFE CFESENMEMTA GPKVPEIELILQSEMVGWKIYGRNSMVKVND+A+CLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDKAYCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NAIVLGGYQMEDIVLDFD+GTSMLGFSSSLLQRKRSCSEFSPENLL+RTG
Sbjct: 361 DGGLKPRNAIVLGGYQMEDIVLDFDIGTSMLGFSSSLLQRKRSCSEFSPENLLKRTG 417

BLAST of PI0009331 vs. NCBI nr
Match: XP_038875838.1 (probable aspartic proteinase GIP2 [Benincasa hispida])

HSP 1 Score: 726.5 bits (1874), Expect = 1.3e-205
Identity = 386/417 (92.57%), Postives = 396/417 (94.96%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MA  HSPFLLLLLL    LTLF G TFSLVIPLTKDS+TN YLATV+HGSPIKP+HLAVD
Sbjct: 1   MAGTHSPFLLLLLLCF--LTLFHGSTFSLVIPLTKDSVTNHYLATVYHGSPIKPLHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGGQSLWMACG SSSSRSIPSRSIQCIAATGGGRSGSVGG CDVIA NPFGDLEGKA+LV
Sbjct: 61  LGGQSLWMACGASSSSRSIPSRSIQCIAATGGGRSGSVGGVCDVIARNPFGDLEGKAVLV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VA+RSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQ+ATELGS+
Sbjct: 121 EDTVALRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQIATELGSH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFSICLSST GVVFPDSGSQDSVYGSEISSSLTYTPILTK IDASQSPEYFINVKAIKV
Sbjct: 181 RRFSICLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDL+GGGD GGGGGTRLSTVVPYTALESSIFNSL  AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLDGGGD-GGGGGTRLSTVVPYTALESSIFNSLIAAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVA FE CFESENMEMTATGPKVPEIELILQSEMVGWKI GRNSMVKVNDEAFCLGFV
Sbjct: 301 VAPVALFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDEAFCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLL+RTG
Sbjct: 361 DGGLKPRNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKRTG 414

BLAST of PI0009331 vs. NCBI nr
Match: KAG7013583.1 (Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 694.5 bits (1791), Expect = 5.6e-196
Identity = 367/417 (88.01%), Postives = 382/417 (91.61%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MAVP S  LLLLLLL  S  LF G  FSLVIP+ KDS+TNQYLAT++HGSPIKPVHLAVD
Sbjct: 1   MAVPESRLLLLLLLLCFS-ALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGG+ LWMACGGSSSSR I SRSIQCIAA GGGRSGS G ACDVIAGNPFGDLEGKA+LV
Sbjct: 61  LGGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VAVRSLDRSTAAVIVALHSCAPRFLLQGLAKS KG+LGLGRNQISLPAQ+ATELG +
Sbjct: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFS+CLSST GVVFPDSG+ DSVYGSEISSSLTYTPILTK IDASQSPEYFINVKAIKV
Sbjct: 181 RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDL+G   GGGGGGTRLSTVVPYT LESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVAPFE CFESENMEMTATGPKVPEIELILQSEMVGWKI GRNSMVKVNDE  CLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NA+VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRK+SCSEFSPENL  R G
Sbjct: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 416

BLAST of PI0009331 vs. NCBI nr
Match: XP_022959401.1 (basic 7S globulin-like [Cucurbita moschata])

HSP 1 Score: 689.1 bits (1777), Expect = 2.3e-194
Identity = 365/417 (87.53%), Postives = 379/417 (90.89%), Query Frame = 0

Query: 1   MAVPHSPFLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60
           MAVP S  LLLL LLL    LF G  FSLVIP+ KDS+TNQYLAT++HGSPIKPVHLAVD
Sbjct: 1   MAVPESRLLLLLRLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60

Query: 61  LGGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILV 120
           LGGQ LWMACGGSSSSR I SRSIQCIAA GGGRSGS G ACDVIAGNPFGDLEGKA+LV
Sbjct: 61  LGGQFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120

Query: 121 EDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGSN 180
           ED VAVRSLDRSTAAVIVALHSCAPRFLLQGLA+S KG+LGLGRNQISLPAQ+ATELG +
Sbjct: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAESAKGVLGLGRNQISLPAQIATELGFH 180

Query: 181 RRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIKV 240
           RRFS+CLSST GVVFPDSG+ DSVYGSEISSSLTYTPILTK IDASQSPEYFINVKAIKV
Sbjct: 181 RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDL    DGG GGGTRLSTVVPYT LESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDL----DGGSGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFCLGFV 360
           VAPVAPFE CFESENMEMTATGPKVPEIELILQSEMVGWKI GRNSMVKVNDE  CLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360

Query: 361 DGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLERTG 418
           DGGLKP+NA+VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRK+SCSEFSPENL  R G
Sbjct: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 413

BLAST of PI0009331 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 250.8 bits (639), Expect = 2.0e-66
Identity = 157/421 (37.29%), Postives = 237/421 (56.29%), Query Frame = 0

Query: 9   LLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGGQSLWM 68
           LLL +  LSS      +  +L++P+TKD  T QY   +   +P+ P  +  DLGG+ LW+
Sbjct: 11  LLLFIFSLSSSAQTPFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGGRELWV 70

Query: 69  AC--GGSSSSRSIP---------SRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKA 128
            C  G  SS+   P         + S  C       R G     C  I  N         
Sbjct: 71  DCDKGYVSSTYQSPRCNSAVCSRAGSTSCGTCFSPPRPGCSNNTCGGIPDNTVTGTATSG 130

Query: 129 ILVEDMVAVRSLDRSTAAVIV----ALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQL 188
               D+V+++S + S    +V     +  C   FLL+GLAK   G+ G+GR+ I LP+Q 
Sbjct: 131 EFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGLAKGTVGMAGMGRHNIGLPSQF 190

Query: 189 ATELGSNRRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDAS------Q 248
           A     +R+F++CL+S  GV F  +G    + G +ISS  T   ++  +  AS      +
Sbjct: 191 AAAFSFHRKFAVCLTSGKGVAFFGNGPYVFLPGIQISSLQTTPLLINPVSTASAFSQGEK 250

Query: 249 SPEYFINVKAIKVDGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTT 308
           S EYFI V AI++    + +N +LL +       G GGT++S+V PYT LESSI+N+ T+
Sbjct: 251 SSEYFIGVTAIQIVEKTVPINPTLLKINA---STGIGGTKISSVNPYTVLESSIYNAFTS 310

Query: 309 AFRAAAAAMNMKEVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSM 368
            F   AAA ++K VA V PF  CF ++N+ +T  G  VPEIEL+L S+ V W+I+G NSM
Sbjct: 311 EFVKQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIELVLHSKDVVWRIFGANSM 370

Query: 369 VKVNDEAFCLGFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEF 409
           V V+D+  CLGFVDGG+  + ++V+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C+ F
Sbjct: 371 VSVSDDVICLGFVDGGVNARTSVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNCANF 428

BLAST of PI0009331 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 245.0 bits (624), Expect = 1.1e-64
Identity = 159/422 (37.68%), Postives = 236/422 (55.92%), Query Frame = 0

Query: 9   LLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGGQSLWM 68
           LLL +  LSS      +  +L++P+TKD  T QY   +   +P+ P  +  DLGG+  W+
Sbjct: 12  LLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASVVFDLGGREFWV 71

Query: 69  AC--GGSSSSRSIP---------SRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKA 128
            C  G  S++   P         + SI C       R G     C     N         
Sbjct: 72  DCDQGYVSTTYRSPRCNSAVCSRAGSIACGTCFSPPRPGCSNNTCGAFPDNSITGWATSG 131

Query: 129 ILVEDMVAVRSLDRSTAAVIV----ALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQL 188
               D+V+++S + S     V     + SC    LL+GLAK   G+ G+GR+ I LP Q 
Sbjct: 132 EFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLKGLAKGAVGMAGMGRHNIGLPLQF 191

Query: 189 ATELGSNRRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPIL----TKIIDAS--- 248
           A     NR+F++CL+S  GV F  +G    + G +I S L  TP+L    T + + S   
Sbjct: 192 AAAFSFNRKFAVCLTSGRGVAFFGNGPYVFLPGIQI-SRLQKTPLLINPGTTVFEFSKGE 251

Query: 249 QSPEYFINVKAIKVDGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLT 308
           +SPEYFI V AIK+    L ++ +LL +       G GGT++S+V PYT LESSI+ + T
Sbjct: 252 KSPEYFIGVTAIKIVEKTLPIDPTLLKINA---STGIGGTKISSVNPYTVLESSIYKAFT 311

Query: 309 TAFRAAAAAMNMKEVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNS 368
           + F   AAA ++K VA V PF  CF ++N+ +T  G  VPEI+L+L S+ V W+I+G NS
Sbjct: 312 SEFIRQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIQLVLHSKDVVWRIFGANS 371

Query: 369 MVKVNDEAFCLGFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSE 409
           MV V+D+  CLGFVDGG+ P  ++V+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C+ 
Sbjct: 372 MVSVSDDVICLGFVDGGVNPGASVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNCAN 429

BLAST of PI0009331 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 219.2 bits (557), Expect = 6.4e-57
Identity = 158/409 (38.63%), Postives = 209/409 (51.10%), Query Frame = 0

Query: 8   FLLLLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGGQSLW 67
           F   L  L+ S +        +V P+ KD  T QYLA +  G    PV L VDL G  LW
Sbjct: 11  FFSFLSALIISKSQISDSVNGVVFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSILW 70

Query: 68  MACGG---SSSSRSIPSRSIQCIAATGGGRSGSVGGA--------CDVIAGNPFGDLEGK 127
             C     SSS   I   S  C+ A  G    S   +        C+++  N    +  +
Sbjct: 71  FDCSSRHVSSSRNLISGSSSGCLKAKVGNERVSSSSSSRKDQNADCELLVKNDAFGITAR 130

Query: 128 AILVEDMVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATE 187
             L  D+++V S+  ++   +  L +C P +LL+GLA   +G++GLGR QISLP+QLA E
Sbjct: 131 GELFSDVMSVGSV--TSPGTVDLLFACTPPWLLRGLASGAQGVMGLGRAQISLPSQLAAE 190

Query: 188 LGSNRRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVK 247
               RR ++ LS   GVV   + S + V+G   S SL YTP+LT       S  Y INVK
Sbjct: 191 TNERRRLTVYLSPLNGVV--STSSVEEVFGVAASRSLVYTPLLT-----GSSGNYVINVK 250

Query: 248 AIKVDGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAM 307
           +I+V+G +L +   L                LSTVVPYT LESSI+     A+  A AA 
Sbjct: 251 SIRVNGEKLSVEGPL-------------AVELSTVVPYTILESSIYKVFAEAY--AKAAG 310

Query: 308 NMKEVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAFC 367
               V PVAPF +CF S+           P ++L LQSEMV W+I+G+N MV V     C
Sbjct: 311 EATSVPPVAPFGLCFTSD--------VDFPAVDLALQSEMVRWRIHGKNLMVDVGGGVRC 370

Query: 368 LGFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCS 406
            G VDGG    N IV+GG Q+E  +LDFD+G SM+GF     QR RS S
Sbjct: 371 SGIVDGGSSRVNPIVMGGLQLEGFILDFDLGNSMMGFG----QRTRSDS 383

BLAST of PI0009331 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.8e-44
Identity = 141/409 (34.47%), Postives = 201/409 (49.14%), Query Frame = 0

Query: 11  LLLLLLSSLTLFLGQTFSLVIPLTKDSLTNQYLATVFHGSPIK-PVHLAVDLGGQSLWMA 70
           LL+ L     + L      ++P+TK   TN +  T   GS  K PV+L +DLG    W+ 
Sbjct: 8   LLVFLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLGTNLTWLD 67

Query: 71  C---GGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGD---LEGKAILVEDM 130
           C      SS R +  +S  C +  G   +G  G +C     NP G    + G+  +V+D 
Sbjct: 68  CRKLKSLSSLRLVTCQSSTCKSIPG---NGCAGKSCLYKQPNPLGQNPVVTGR--VVQDR 127

Query: 131 VAVRSLD----RSTAAVIVALHSCAPRFLLQGLAKSVKGILGLGRNQISLPAQLATELGS 190
            ++ + D     S  +V     SCA    LQGL   V G+L L     S   Q+ +    
Sbjct: 128 ASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGLPPPVDGVLALSPGSSSFTKQVTSAFNV 187

Query: 191 NRRFSICLSSTYGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDASQSPEYFINVKAIK 250
             +FS+CL S+    F  +G     +    +SS    P     I  + S +Y I VK+I 
Sbjct: 188 IPKFSLCLPSSGTGHFYIAGIH--YFIPPFNSSDNPIPRTLTPIKGTDSGDYLITVKSIY 247

Query: 251 VDGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSIFNSLTTAFRAAAAAMNMK 310
           V G  L LN  LL           GG +LSTVV YT L++ I+N+L  +F   A AM + 
Sbjct: 248 VGGTALKLNPDLLT----------GGAKLSTVVHYTVLQTDIYNALAQSFTLKAKAMGIA 307

Query: 311 EVAPVAPFEVCFESENMEMTAT-GPKVPEIELILQSEM--VGWKIYGRNSMVKVNDEAFC 370
           +V  VAPF+ CF+S       T GP VP IE+ L   +  V W  YG N++VKV +   C
Sbjct: 308 KVPSVAPFKHCFDSRTAGKNLTAGPNVPVIEIGLPGRIGEVKWGFYGANTVVKVKETVMC 367

Query: 371 LGFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCS 406
           L F+DGG  PK+ +V+G +Q++D +L+FD   ++L FS SLL    SCS
Sbjct: 368 LAFIDGGKTPKDLMVIGTHQLQDHMLEFDFSGTVLAFSESLLLHNTSCS 399

BLAST of PI0009331 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 130.2 bits (326), Expect = 3.9e-30
Identity = 124/424 (29.25%), Postives = 189/424 (44.58%), Query Frame = 0

Query: 11  LLLLLLSSLTLFLGQTF-------SLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVDLGG 70
           ++ LLLS + L+L  T        S + P+ KD+  N Y   +  GS        +DL G
Sbjct: 5   VIFLLLSLVFLYLANTSHSLRKFQSFLHPIYKDTAKNIYTIPLSIGS-TSSEKFVLDLNG 64

Query: 71  QS-LWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILVED 130
            + L   C   ++++S     I+C             G+      NP        I  + 
Sbjct: 65  AAPLLQNC--PTAAKSTTYHPIRC-------------GSTRCKYANPNFPCPNNVIAKKR 124

Query: 131 MVAVRS----LDRSTAAVIVALHSCAPR----------FLLQGLAKSVKGILGLGRNQIS 190
            V + S    L R T  ++   +    R              G     +  +GL    +S
Sbjct: 125 TVCLSSDNSRLFRDTVPLLYTFNGVYTRDSEMSSSLTLTCTDGAPALKQRTIGLANTHLS 184

Query: 191 LPAQLATELGSNRRFSICLSST------YGVVFPDSGSQDSV-YGSEISSSLTYTPILTK 250
           +P+QL +      + ++CL ST       G ++   G    + Y  ++S     TP    
Sbjct: 185 IPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIGKGEYYYLPYDKDVSKIFASTP---- 244

Query: 251 IIDASQSPEYFINVKAIKVDGNRLDLNKSLLDLEGGGDGGGGGGTRLSTVVPYTALESSI 310
           +I   +S EY I+VK+I++    + +                G T++ST+ PYT  ++S+
Sbjct: 245 LIGNGKSGEYLIDVKSIQIGAKTVPIPY--------------GATKISTLAPYTVFQTSL 304

Query: 311 FNSLTTAFRAAAAAMNMKEVAPVAPFEVCFESENMEMTATGPKVPEIELILQSEMVGWKI 370
           + +L TAF      + + +   V PF  CF S        G  VP I+L+L S    W+I
Sbjct: 305 YKALLTAF---TENIKIAKAPAVKPFGACFYSNG------GRGVPVIDLVL-SGGAKWRI 364

Query: 371 YGRNSMVKVNDEAFCLGFVDGGLKPKNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRK 406
           YG NS+VKVN    CLGFVDGG+KPK  IV+GG+QMED +++FD+  S   FSSSLL   
Sbjct: 365 YGSNSLVKVNKNVVCLGFVDGGVKPKYPIVIGGFQMEDNLVEFDLEASKFSFSSSLLLHN 384

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0DO211.7e-7040.00Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 ... [more]
I1JNS63.9e-5936.54Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2[more]
P829525.7e-5034.27Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2[more]
Q9FSH94.3e-4232.27Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1[more]
Q423691.1e-4032.92Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KHJ89.2e-21394.48Basic 7S globulin 2 small subunit OS=Cucumis sativus OX=3659 GN=Csa_6G237600 PE=... [more]
A0A5A7SQG36.6e-21193.53Basic 7S globulin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3C7N96.6e-21193.53basic 7S globulin-like OS=Cucumis melo OX=3656 GN=LOC103497928 PE=4 SV=1[more]
A0A6J1H4R81.1e-19487.53basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460386 PE=4 SV=1[more]
A0A6J1L1S18.1e-19387.05basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111499071 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004151001.11.9e-21294.48probable aspartic proteinase GIP2 [Cucumis sativus] >KGN47256.1 hypothetical pro... [more]
XP_008458562.11.4e-21093.53PREDICTED: basic 7S globulin-like [Cucumis melo] >KAA0033422.1 basic 7S globulin... [more]
XP_038875838.11.3e-20592.57probable aspartic proteinase GIP2 [Benincasa hispida][more]
KAG7013583.15.6e-19688.01Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022959401.12.3e-19487.53basic 7S globulin-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G03220.12.0e-6637.29Eukaryotic aspartyl protease family protein [more]
AT1G03230.11.1e-6437.68Eukaryotic aspartyl protease family protein [more]
AT5G19120.16.4e-5738.63Eukaryotic aspartyl protease family protein [more]
AT5G19110.12.8e-4434.47Eukaryotic aspartyl protease family protein [more]
AT5G19100.13.9e-3029.25Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 26..196
e-value: 8.2E-30
score: 106.1
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 204..410
e-value: 2.2E-59
score: 202.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 34..398
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 230..394
e-value: 4.8E-52
score: 176.0
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 42..189
e-value: 5.8E-24
score: 85.3
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 9..407
NoneNo IPR availablePANTHERPTHR47965:SF46BASIC 7S GLOBULIN 2 SMALL SUBUNITcoord: 9..407
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 42..394
score: 19.840481

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009331.1PI0009331.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity