PI0009172 (gene) Melon (PI 482460) v1

Overview
NamePI0009172
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionUracil-DNA glycosylase
Locationchr10: 9364663 .. 9369779 (+)
RNA-Seq ExpressionPI0009172
SyntenyPI0009172
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGAACCCATCGACCATACGAAAGGACGTAACGAGGCAAATCCCATACAATCAATTTCAAACCATCTTGGAGAGATGAACGCTTTATAGCGGGATTTTCCCTTGCTTCCCGCCATTGAAGCACACTCCTCTCAGCTGCTACCAACCACACCTACAATGGCTTCTTCCTCCGCTTCACTCTCATCCAAAACTAGAACCCTAATCGACATCTTCCAGCCAGCGCTTTCCAAACGCTTAAAAACCTCACAGACATTGAAAACCCTAGCAACCACCGACGACAAATGCGATTCAGACCTCACATTGGCTTCCTCCTCCACTGACATGTCTGCCTCTCAGATATCCCGCATGGAAACCAACAAATGGATGGCCAGATCGAAGCGCAATCTCAAAATTTGCTCAGATAGGGTCTCCAAATGGGAGAATGGATGTGTGAAGTTGGAGGAGCTTTTGGTGGAGGAGACATGGTTCGAAGCTCTTCCTGGAGAGTTCGAGAAGCCCTATGCTCTTAATCTTTGCAAATTTGTACAGACAGAGATTTGCAGTAGTGGTGTCCCTATTTATCCTCCTCCTTCTTTGATCTTTAATGCTCTGAATTCTACCCCTTTTGATAGGGTCAAAGTTGTTATTCTTGGTCAAGATCCTTATCATGGGCCTGGTCAAGCTATGGGTCTTTCATTTTCTGTTCCCGAGGGTGTTAAAATCCCATCTAGTCTTCTCAACATATTCAAGGAACTGAGGAATGATCTTGGTTGTTCCATCCCATCCCATGGAAATCTCGAGAAATGGGCTGTTCAGGTGCTGTTGTCTTTCATCTCTTTGTTCTGTAGACTTAGATATGCTTGCTTAAGTGTATCTACTTCTCTAATATATTGGTATTGTGAATTTTTTGGTGCTGCATAAATGCTATTTACCGCTGGTTAAAGTAGATGACATGGAGGTCTTGCTTATTTATCATTAGATGAAAGTTATGTGAAAATAGGTTTGGGACACAGATGAACTGTACAATCCATGGTCCTCAATTCGCCTTTTGGGGGAGGAAGTCCGTTTTTTTTTATGGCTTGCTGGGTTTGTGCAGTCTTATGGGTCTTAAGGGGTGAGTGGAACATGTTTAGGGGAGTGGAGAAGGGATCCCAAGGAGTTATGGATATTTTTCTCAATGTGTATGGATATGGTTCCTATCACAAGGTTGTGATTTTGGTGATTGCTTAGGGAATGAGGAAATATTTTCGGCATTGAATGCCTTTTCTTTGATTAAAAACTTTACTTCTGAAGACAGCTAATAAATTATGCTCGTTTCAATTCATTCATTTATTAATTGTTATTATTTGAAAACTTGTTGATAAGTCGAGAATTTGGGTTGTTACTAGGTGACTTCTCTAAGTTGAGATATCGTATCATACAATTATTAGTTCTTATTGAAGCATTCTAAGTGTGGTTCGTCATGACATTACTCTTATGAACGTGGACTTGAATGGATTCGTAGGAAAGTTGATCGTTTCAGGAGTTTTCTGCATGGAAAAATGCATGATTAGTTATTTTCTGAATAATTATTTTGTTAGGATATATTTGAAGTCCTATCTGGTGTCTTTTTCATTTTTTTGTGCTCTAAAATGATTTTTATGTACATTGTAGGGAGTCCTGTTGCTTAATGCTGTTCTCTCAGGTAATTTCCACCCAAGTTTACCCCTTCTTTCCTCCGTCTACTTTTATTTTTTAAATTAGTAAAGTGATGGTGAAGGTATGATCAAAATGCTTCGTTTTGCTCTTTGTGATTGTTTTTAAACAAGTATTTCACAATGAGTAACTTTGACCATCTTAAAAGCTTTGTAATAGCTACATGATAGTTTAAATTTCCAGCTCCAAAGAATAATAGCGAACTTTAATGCCATCAGCAACATCTAGGATATCGAACCAATAACTCTTCAAACACCACCAAACTGTGATGAAGTGACATGGCTAACATAGATAGTCATATCTTCAAAGCCAGAAGAGAAATTAGCTACAACTAAAGCAAAATTGTTATCATTCTTAAGCTGCAAACCTTTTCCTAGGAGAAAAATAGCAACAGGGCACCAAATGGGTGATCCTTGCCAAAGAATTTTGTAAAGTTGTAACATTTCATTACTGGAATGCAAGTCCTCGGGGAACAAGCTAACAAATGCACCAGTCCTCAAAATAGCCTTAAAAAAACATCAGACCCAAAGAAGGCTAAAGTGCCCTGTCCAAGATTGCCATAAACAAACCACAACGAACCGTAAACAAACTGAAACCTAGACTTTGGATAGACAGTTGGTAAGAATCAACTGCTTGAGAAGAAATACTGTAAAGGGTGGAGTTTCTTGTTCTGTATCATTTCTATCACATATTCAAATTTAGGGTTTCTTTAAAGTGGCCTTATGTCCATCAAACTTTAATAAGAGTTCTATATTATTCTATTTTGACGTTAGGATACATGCTTCCTGCATTTAGTTATTGATTAATATTTTGGTTTTACCTGTTTAGTTCGAAAGCATCAAGCAAACTCTCATGCTAAGAGAGGATGGGAACAATTCACTGATGCTGTTATCAAGACAATATCACAAAAAAAGGAAGGGATTGTCTTTTTACTTTGGGGGAACTCCGCTCAAGCGAAATTGAGGTACTTCTGTCATATGATATTAGTTCTAAGTTTCTAACAGTGAGTTCACGACTCCGGAAAGAACCAAACTAATAACAGAATCAAAATTAAAATAAAAATTGCAACCTTTAGATTTTTCTTGTTTGAAAATAAGAAAATCAATGACCTTTCATTATTTTTTTTGCATCTAGCATGCATCTTTCATACATTGATCAAAATCTGGCATAACAAATCTTAATGGGAAAAGAAAACAAGGACCGAGGGCTTTCAGTTGAAGACTTGAAGTATTTGATAGATAAAATGGGATGAGATATTAGATAATTAGTGTAATTCAGTTAATATAAGTCTAGTTGTTGACTCTTAGATTAACGTGATTGATTAATATTTGGGATTTATTTCTTTGTCTGTTAATTTTATCATAAGACTGTCAATATCACTTTTTAGGACTGTAAGGAAGCTTTTGAATATTATTTCAAAGTAAACAACAAAATAAAACCTACATGATTGAAGTACTTTGAGAGGCTAGGAGGTCTCACTCAAAGACCATTTCCCAAAGTTTGTTTACTTCAAAAAACTGTTCATTTTATTTCTAACCACTCAACTTTGACGAGCTTATATTAAGAATTACAATGAATATGCATGCCATTGGTAATATTCCCCTTTCTCAATTGCATTTCATATTAATAGTTCACAAATTAAAAGCCTCACGTGAATGTAAAGTGCACACCCACACATGGGGGATGTGTTGTAAATACTTTACCATAGTAGTCATTTCCTACCTATGTTGTTATGCAAGCTTTAATCTTTCAAGTATAGGAACTTTTCCTGTGAAGTACATGAAACTATTTGGGAAAACATGGTTTTGCTGAGTTTTAAAATCAACATAGTGCAATTTTCATCTACTTTAGCTATCTAACGTTCTATTGTCTTGATAAAACTTACAAGAGCATCACCAAAGAGATTTAATTCCTCATCTTTGGGGGGCAATAACATCGTACTTCACTTAGAAGCTAATGTATGATGTGACCAGGTTAATTGATGAGAAAAAGCATCACATTCTCAAAGCAGCGCATCCTTCTGGTTTGTCTGCTAACAGAGGCTTCTTTGGTTGCAGGTTAGTTTCATCGTTTAATTCCATCATACACTTTTTAAACCTTCCTTTTCTTAAAATATTAACTAGGATTTGCTCTACCTCTAGCCATTGTATTTTATCATTTCCGTTCAGTATAGTTCTCACAATATCAACATTTTATTGCCAGTGCATAGCTTGGTATTTTGTGTATATCACTTGGTTTTCTGCCTTCTCTCTAAATTAAACTTATTATGTTGGACCACTTCTTTGCTCGATTTTATAAGTGATTATGGCCGGAGCGTGAATTTGATATGATTTTTCTGATTGTGAAAAAAATATTTAGCCTATCCCAAACAAACATTTTCGAACTTTTTATGTAGTTTTGATTTCATAGTAAAAAATGATTGAAAAGAGTTCTCAAAATACATAAAAGCACTTTTGGAACATAGTTGTTTATAGATAAAATGTGACTTAAAAGTATTTACTCTCGAGAATCATACCAAACTCCCACTTACCTTTTAGGCTCTTCCCTTAAGTTCTGTAGTCCATCTCTCAGAACATAGACAATTTTGTGAGGCTGTTGAAGTTTATAATCATAAGAGATTATATGTTTATGTAATTCACTAGTATGAAGCTATTCAGATGGGTGGTTTTTCATTTCGTTTGGAATTTTAGTTTGTTTACTGCCAACTTTGGATTTTCGACAGGCATTTTTCTCGAACAAACATACTTCTCAAGGAACTAGGTACTGCTTCCATAGATTGGCAACTTTGATCCAAGCATGTGAACCATTGAAGTTCATTTTTTGTGGATACACTTCAAGATCCCCAGTCATGCTCAGACCTGCTTGATCGATGACTTTTTGTTGAATTTTCACTTGTCAATTCACCGTTGTCTAAATATGGAGATGCCCATCCTTTACCTTTTTGTATTATAGTGTTTATTCCTGCTGTTTGATATTTATCCCAAAGCAGCAGCAGGACCCTTCTTTTTATAAAATAGGGTAAAATTGGAAGTTAGTCTATCATATGGTGACCGGTGGTTATCATAAATCAGGCTAGATGTCTGGTTGTACCTAACTTTTGGCTGTTTTCTCAATCCTATCGTGGGAAGTGGGAAGATTCATTTTAGTCCCTGTTGTTGGTAAAATATGGGCAGAAAACTGACTGCCCTTCATGTGCTTGTTATAATTTTGGAGAAAGATACTTGCAAAACAAAGAAAAAGAACACATGTACGGTGGCAACCATAGGACCTCCAACCTCCGATGCTTAAGTCAATCCAGGGAGCAAACTAATAGTAGGAAAAATATGTTACCTTTTTTGGTTTTATGATCTCTTATTTATTGAGGGGTGAACCGGAGATGATGATTGGAGCTTATCAGAGTCTTAGAGCATGGGCCACAAGAAATGGGCTCTCGTGAAAGGAAG

mRNA sequence

ATTGAACCCATCGACCATACGAAAGGACGTAACGAGGCAAATCCCATACAATCAATTTCAAACCATCTTGGAGAGATGAACGCTTTATAGCGGGATTTTCCCTTGCTTCCCGCCATTGAAGCACACTCCTCTCAGCTGCTACCAACCACACCTACAATGGCTTCTTCCTCCGCTTCACTCTCATCCAAAACTAGAACCCTAATCGACATCTTCCAGCCAGCGCTTTCCAAACGCTTAAAAACCTCACAGACATTGAAAACCCTAGCAACCACCGACGACAAATGCGATTCAGACCTCACATTGGCTTCCTCCTCCACTGACATGTCTGCCTCTCAGATATCCCGCATGGAAACCAACAAATGGATGGCCAGATCGAAGCGCAATCTCAAAATTTGCTCAGATAGGGTCTCCAAATGGGAGAATGGATGTGTGAAGTTGGAGGAGCTTTTGGTGGAGGAGACATGGTTCGAAGCTCTTCCTGGAGAGTTCGAGAAGCCCTATGCTCTTAATCTTTGCAAATTTGTACAGACAGAGATTTGCAGTAGTGGTGTCCCTATTTATCCTCCTCCTTCTTTGATCTTTAATGCTCTGAATTCTACCCCTTTTGATAGGGTCAAAGTTGTTATTCTTGGTCAAGATCCTTATCATGGGCCTGGTCAAGCTATGGGTCTTTCATTTTCTGTTCCCGAGGGTGTTAAAATCCCATCTAGTCTTCTCAACATATTCAAGGAACTGAGGAATGATCTTGGTTGTTCCATCCCATCCCATGGAAATCTCGAGAAATGGGCTGTTCAGGGAGTCCTGTTGCTTAATGCTGTTCTCTCAGTTCGAAAGCATCAAGCAAACTCTCATGCTAAGAGAGGATGGGAACAATTCACTGATGCTGTTATCAAGACAATATCACAAAAAAAGGAAGGGATTGTCTTTTTACTTTGGGGGAACTCCGCTCAAGCGAAATTGAGGTTAATTGATGAGAAAAAGCATCACATTCTCAAAGCAGCGCATCCTTCTGGTTTGTCTGCTAACAGAGGCTTCTTTGGTTGCAGGCATTTTTCTCGAACAAACATACTTCTCAAGGAACTAGGTACTGCTTCCATAGATTGGCAACTTTGATCCAAGCATGTGAACCATTGAAGTTCATTTTTTGTGGATACACTTCAAGATCCCCAGTCATGCTCAGACCTGCTTGATCGATGACTTTTTGTTGAATTTTCACTTGTCAATTCACCGTTGTCTAAATATGGAGATGCCCATCCTTTACCTTTTTGTATTATAGTGTTTATTCCTGCTGTTTGATATTTATCCCAAAGCAGCAGCAGGACCCTTCTTTTTATAAAATAGGGTAAAATTGGAAGTTAGTCTATCATATGGTGACCGGTGGTTATCATAAATCAGGCTAGATGTCTGGTTGTACCTAACTTTTGGCTGTTTTCTCAATCCTATCGTGGGAAGTGGGAAGATTCATTTTAGTCCCTGTTGTTGGTAAAATATGGGCAGAAAACTGACTGCCCTTCATGTGCTTGTTATAATTTTGGAGAAAGATACTTGCAAAACAAAGAAAAAGAACACATGTACGGTGGCAACCATAGGACCTCCAACCTCCGATGCTTAAGTCAATCCAGGGAGCAAACTAATAGTAGGAAAAATATGTTACCTTTTTTGGTTTTATGATCTCTTATTTATTGAGGGGTGAACCGGAGATGATGATTGGAGCTTATCAGAGTCTTAGAGCATGGGCCACAAGAAATGGGCTCTCGTGAAAGGAAG

Coding sequence (CDS)

ATGGCTTCTTCCTCCGCTTCACTCTCATCCAAAACTAGAACCCTAATCGACATCTTCCAGCCAGCGCTTTCCAAACGCTTAAAAACCTCACAGACATTGAAAACCCTAGCAACCACCGACGACAAATGCGATTCAGACCTCACATTGGCTTCCTCCTCCACTGACATGTCTGCCTCTCAGATATCCCGCATGGAAACCAACAAATGGATGGCCAGATCGAAGCGCAATCTCAAAATTTGCTCAGATAGGGTCTCCAAATGGGAGAATGGATGTGTGAAGTTGGAGGAGCTTTTGGTGGAGGAGACATGGTTCGAAGCTCTTCCTGGAGAGTTCGAGAAGCCCTATGCTCTTAATCTTTGCAAATTTGTACAGACAGAGATTTGCAGTAGTGGTGTCCCTATTTATCCTCCTCCTTCTTTGATCTTTAATGCTCTGAATTCTACCCCTTTTGATAGGGTCAAAGTTGTTATTCTTGGTCAAGATCCTTATCATGGGCCTGGTCAAGCTATGGGTCTTTCATTTTCTGTTCCCGAGGGTGTTAAAATCCCATCTAGTCTTCTCAACATATTCAAGGAACTGAGGAATGATCTTGGTTGTTCCATCCCATCCCATGGAAATCTCGAGAAATGGGCTGTTCAGGGAGTCCTGTTGCTTAATGCTGTTCTCTCAGTTCGAAAGCATCAAGCAAACTCTCATGCTAAGAGAGGATGGGAACAATTCACTGATGCTGTTATCAAGACAATATCACAAAAAAAGGAAGGGATTGTCTTTTTACTTTGGGGGAACTCCGCTCAAGCGAAATTGAGGTTAATTGATGAGAAAAAGCATCACATTCTCAAAGCAGCGCATCCTTCTGGTTTGTCTGCTAACAGAGGCTTCTTTGGTTGCAGGCATTTTTCTCGAACAAACATACTTCTCAAGGAACTAGGTACTGCTTCCATAGATTGGCAACTTTGA

Protein sequence

MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLCKFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL
Homology
BLAST of PI0009172 vs. ExPASy Swiss-Prot
Match: Q9LIH6 (Uracil-DNA glycosylase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=UNG PE=1 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 3.7e-110
Identity = 209/330 (63.33%), Postives = 247/330 (74.85%), Query Frame = 0

Query: 9   SSKTRTLIDIFQPALSKRLKT---------------SQTLKTLATTDDKCDSDLTLASSS 68
           SS  +TL+D FQPA  KRLK                S+ L ++A +  +     ++A  S
Sbjct: 3   SSTPKTLMDFFQPA--KRLKASPSSSSFPAVSVAGGSRDLGSVANSPPRVTVTTSVADDS 62

Query: 69  TDMSASQISRMETNKWMARSKRNLKICSDRV--SKWENGC-VKLEELLVEETWFEALPGE 128
           + ++  QI+R E NK++A+SKRNL +CS+RV  +K E  C V L ELLVEE+W +ALPGE
Sbjct: 63  SGLTPEQIARAEFNKFVAKSKRNLAVCSERVTKAKSEGNCYVPLSELLVEESWLKALPGE 122

Query: 129 FEKPYALNLCKFVQTEIC--SSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQ 188
           F KPYA +L  F++ EI   S    IYPP  LIFNALN+TPFDRVK VI+GQDPYHGPGQ
Sbjct: 123 FHKPYAKSLSDFLEREIITDSKSPLIYPPQHLIFNALNTTPFDRVKTVIIGQDPYHGPGQ 182

Query: 189 AMGLSFSVPEGVKIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQ 248
           AMGLSFSVPEG K+PSSLLNIFKEL  D+GCSIP HGNL+KWAVQGVLLLNAVL+VR  Q
Sbjct: 183 AMGLSFSVPEGEKLPSSLLNIFKELHKDVGCSIPRHGNLQKWAVQGVLLLNAVLTVRSKQ 242

Query: 249 ANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLS 308
            NSHAK+GWEQFTDAVI++ISQ+KEG+VFLLWG  AQ K +LID  KHHIL AAHPSGLS
Sbjct: 243 PNSHAKKGWEQFTDAVIQSISQQKEGVVFLLWGRYAQEKSKLIDATKHHILTAAHPSGLS 302

Query: 309 ANRGFFGCRHFSRTNILLKELGTASIDWQL 319
           ANRGFF CRHFSR N LL+E+G   IDWQL
Sbjct: 303 ANRGFFDCRHFSRANQLLEEMGIPPIDWQL 330

BLAST of PI0009172 vs. ExPASy Swiss-Prot
Match: C1DQR0 (Uracil-DNA glycosylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) OX=322710 GN=ung PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.4e-72
Identity = 131/224 (58.48%), Postives = 164/224 (73.21%), Query Frame = 0

Query: 95  EELLVEETWFEALPGEFEKPYALNLCKFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVK 154
           + + +E +W EAL  EFEKPY   L  F++ E  ++G  IYPP SLIFNAL+STP D+VK
Sbjct: 6   DRVRLEASWKEALHDEFEKPYMQELSDFLRRE-KAAGKEIYPPGSLIFNALDSTPLDQVK 65

Query: 155 VVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQG 214
           VVI+GQDPYHGPGQA GL FSV  GV +P SL NIFKEL+ DL   IP HG+L++WA QG
Sbjct: 66  VVIIGQDPYHGPGQAHGLCFSVQPGVPVPPSLQNIFKELKRDLNIDIPKHGHLQRWAEQG 125

Query: 215 VLLLNAVLSVRKHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEK 274
           VLLLN  L+V +  A SHA  GW++FTD VI+ +SQ++E +VF+LWG+ AQ+K RLID  
Sbjct: 126 VLLLNTSLTVERGNAGSHAGMGWQRFTDRVIEVVSQRREHVVFMLWGSHAQSKRRLIDSS 185

Query: 275 KHHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL 319
           KH +L +AHPS LSA+RGF G  HFSR N  L++ G   IDW L
Sbjct: 186 KHLVLCSAHPSPLSAHRGFIGNGHFSRANQFLEQHGLTPIDWHL 228

BLAST of PI0009172 vs. ExPASy Swiss-Prot
Match: A7I0A8 (Uracil-DNA glycosylase OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) OX=360107 GN=ung PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 8.9e-72
Identity = 138/227 (60.79%), Postives = 165/227 (72.69%), Query Frame = 0

Query: 92  VKLEELLVEETWFEALPGEFEKPYALNLCKFVQTEICSSGVPIYPPPSLIFNALNSTPFD 151
           +KLE + +E++W E L GEF  PY L + K     + +SGV IYPP +LIFNA N TPFD
Sbjct: 3   IKLENIKIEKSWKEVLKGEFLSPYFLEI-KEKLVCLKNSGVTIYPPGNLIFNAFNLTPFD 62

Query: 152 RVKVVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELRNDLGCSIPSHGNLEKWA 211
           +VKVVILGQDPYH   QAMGLSFSVP+ V+IP SL+NIFKE+ +DLG + P+ G+L  WA
Sbjct: 63  KVKVVILGQDPYHEVNQAMGLSFSVPKDVRIPPSLINIFKEINSDLGINEPNCGDLTFWA 122

Query: 212 VQGVLLLNAVLSVRKHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLI 271
            QGVLLLNA LSV    ANSH   GW+ FTDAVIKT+SQK+E IVF+LWGN A+AK  LI
Sbjct: 123 KQGVLLLNASLSVSAKIANSHKNFGWQIFTDAVIKTLSQKRENIVFMLWGNFAKAKATLI 182

Query: 272 DEKKHHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL 319
           D KKH IL AAHPS L A   FFGC+HFS+ N  L   G + I+W L
Sbjct: 183 DAKKHLILTAAHPSPL-AGGAFFGCKHFSKCNNFLISRGISPINWDL 227

BLAST of PI0009172 vs. ExPASy Swiss-Prot
Match: Q88N05 (Uracil-DNA glycosylase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) OX=160488 GN=ung PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.5e-71
Identity = 129/224 (57.59%), Postives = 161/224 (71.88%), Query Frame = 0

Query: 95  EELLVEETWFEALPGEFEKPYALNLCKFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVK 154
           + + +E +W  AL GEF++PY   L +F++ E  ++G  IYPP  LIFNALNSTP D+VK
Sbjct: 5   DRIKLEPSWKAALRGEFDQPYMHQLREFLRGEY-AAGKEIYPPGPLIFNALNSTPLDQVK 64

Query: 155 VVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQG 214
           VVILGQDPYHGPGQA GL FSV  GV  P SL+NI+KEL+ DL   IPSHG L+ WA QG
Sbjct: 65  VVILGQDPYHGPGQAHGLCFSVQPGVATPPSLVNIYKELQRDLNIPIPSHGYLQSWAEQG 124

Query: 215 VLLLNAVLSVRKHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEK 274
           VLLLN  ++V +  A SHAK+GWE FTD +I+ +S++   +VFLLWG  AQ+K +LID  
Sbjct: 125 VLLLNTTMTVERANAASHAKKGWELFTDRIIQVVSEQCPNVVFLLWGAHAQSKQKLIDGT 184

Query: 275 KHHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL 319
           KH +LK+ HPS LSA RGF GC HFSR N  L++ G   IDW L
Sbjct: 185 KHLVLKSVHPSPLSAYRGFIGCGHFSRANSFLEQRGLGPIDWAL 227

BLAST of PI0009172 vs. ExPASy Swiss-Prot
Match: A5W8H2 (Uracil-DNA glycosylase OS=Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1) OX=351746 GN=ung PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.6e-71
Identity = 129/224 (57.59%), Postives = 161/224 (71.88%), Query Frame = 0

Query: 95  EELLVEETWFEALPGEFEKPYALNLCKFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVK 154
           + + +E +W  AL GEF++PY   L +F++ E  ++G  IYPP  LIFNALNSTP  +VK
Sbjct: 5   DRIKLEPSWKAALRGEFDQPYMHQLREFLRGEY-AAGKEIYPPGPLIFNALNSTPLGQVK 64

Query: 155 VVILGQDPYHGPGQAMGLSFSVPEGVKIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQG 214
           VVILGQDPYHGPGQA GL FSV  GV  P SL+NI+KEL+ DL   IPSHG L+ WA QG
Sbjct: 65  VVILGQDPYHGPGQAHGLCFSVQPGVATPPSLVNIYKELQRDLNIPIPSHGYLQSWAEQG 124

Query: 215 VLLLNAVLSVRKHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEK 274
           VLLLN  ++V +  A SHAK+GWE FTD +I+ +S++   +VFLLWG  AQ+K +LID  
Sbjct: 125 VLLLNTTMTVERANAASHAKKGWELFTDRIIQVVSEQCPNVVFLLWGAHAQSKQKLIDGT 184

Query: 275 KHHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL 319
           KH +LK+ HPS LSA RGF GC HFSRTN  L++ G   IDW L
Sbjct: 185 KHLVLKSVHPSPLSAYRGFIGCGHFSRTNSFLEQRGLGPIDWAL 227

BLAST of PI0009172 vs. ExPASy TrEMBL
Match: A0A1S3CPW3 (Uracil-DNA glycosylase OS=Cucumis melo OX=3656 GN=LOC103502882 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.4e-176
Identity = 312/318 (98.11%), Postives = 316/318 (99.37%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT DDKCDSDLTLASSSTDMSASQ
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSTDMSASQ 60

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
           ISRMETNKWMARSKRNLKICSDRVSKWENGC+KLEELLVEETWFEALPGEFEKPYALNLC
Sbjct: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCMKLEELLVEETWFEALPGEFEKPYALNLC 120

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240
           KIPSSLLNIFKEL++DLGCSIPSHGNL KWAVQGVLLLNAVLSVR+HQANSHAKRGWEQF
Sbjct: 181 KIPSSLLNIFKELKDDLGCSIPSHGNLGKWAVQGVLLLNAVLSVREHQANSHAKRGWEQF 240

Query: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNILLKELGTASIDWQL 319
           RTNILLKELGTASIDWQL
Sbjct: 301 RTNILLKELGTASIDWQL 318

BLAST of PI0009172 vs. ExPASy TrEMBL
Match: A0A0A0KMG3 (Uracil-DNA glycosylase OS=Cucumis sativus OX=3659 GN=Csa_5G289610 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 9.9e-175
Identity = 308/318 (96.86%), Postives = 314/318 (98.74%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT DDKCDSDLTLASSS D+SASQ
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSADISASQ 60

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
           ISRMETNKW+ARSKRNLK CSDRVSKWENGCVKLEELLVEETWFEALPGEF+KPYALNLC
Sbjct: 61  ISRMETNKWIARSKRNLKTCSDRVSKWENGCVKLEELLVEETWFEALPGEFQKPYALNLC 120

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240
           KIPSSLLNIFKELR+DLGCSIPSHGNL KWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF
Sbjct: 181 KIPSSLLNIFKELRDDLGCSIPSHGNLGKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240

Query: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVIKTISQKKEGI+FLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNILLKELGTASIDWQL 319
           RTNILLKE+GTASIDWQL
Sbjct: 301 RTNILLKEMGTASIDWQL 318

BLAST of PI0009172 vs. ExPASy TrEMBL
Match: A0A1S3CND7 (Uracil-DNA glycosylase OS=Cucumis melo OX=3656 GN=LOC103502882 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 3.5e-172
Identity = 312/343 (90.96%), Postives = 316/343 (92.13%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT DDKCDSDLTLASSSTDMSASQ
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSTDMSASQ 60

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
           ISRMETNKWMARSKRNLKICSDRVSKWENGC+KLEELLVEETWFEALPGEFEKPYALNLC
Sbjct: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCMKLEELLVEETWFEALPGEFEKPYALNLC 120

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLS----------------- 240
           KIPSSLLNIFKEL++DLGCSIPSHGNL KWAVQGVLLLNAVLS                 
Sbjct: 181 KIPSSLLNIFKELKDDLGCSIPSHGNLGKWAVQGVLLLNAVLSATSRILNQKLFKHHQTV 240

Query: 241 --------VRKHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKK 300
                   VR+HQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKK
Sbjct: 241 MKSNKADIVREHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKK 300

Query: 301 HHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL 319
           HHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL
Sbjct: 301 HHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL 343

BLAST of PI0009172 vs. ExPASy TrEMBL
Match: A0A6J1J2E4 (Uracil-DNA glycosylase OS=Cucurbita maxima OX=3661 GN=LOC111480636 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.4e-168
Identity = 295/318 (92.77%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MASSSASL SKTRTLIDIFQPALSKRLKTSQTLKTLATTD+KCDS+LTLASSS D+S+SQ
Sbjct: 1   MASSSASLKSKTRTLIDIFQPALSKRLKTSQTLKTLATTDEKCDSELTLASSSMDISSSQ 60

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
            SRMETNKW+ARS RNLKI SDRVSKWENGCVKLEELLV+ETWFEALPGEF+KPYALNLC
Sbjct: 61  KSRMETNKWLARSNRNLKISSDRVSKWENGCVKLEELLVDETWFEALPGEFQKPYALNLC 120

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFV+TEICSSGVPIYPPP LIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVETEICSSGVPIYPPPCLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240
           KIPSSLLNIFKELR DLGCSIPSHGNL+KWAVQGVLLLNAVL+VRKHQANSHAK+GWEQF
Sbjct: 181 KIPSSLLNIFKELREDLGCSIPSHGNLKKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQF 240

Query: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVI+TISQKKEG+VFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDAVIQTISQKKEGVVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNILLKELGTASIDWQL 319
           RTN+LLKELG  SIDWQL
Sbjct: 301 RTNVLLKELGIGSIDWQL 318

BLAST of PI0009172 vs. ExPASy TrEMBL
Match: A0A6J1EEH8 (Uracil-DNA glycosylase OS=Cucurbita moschata OX=3662 GN=LOC111433476 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.4e-167
Identity = 294/318 (92.45%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MASSSASL SKTRTLIDIFQPALSKR+KTSQTLKTLATTD+K DS+LTLASSS D+S+SQ
Sbjct: 1   MASSSASLKSKTRTLIDIFQPALSKRIKTSQTLKTLATTDEKGDSELTLASSSMDISSSQ 60

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
            SRMETNKW+ARSKRNLKI SDRVSKWENGCVKLEELLV+ETWFEALPGEF+KPYALNLC
Sbjct: 61  KSRMETNKWLARSKRNLKISSDRVSKWENGCVKLEELLVDETWFEALPGEFDKPYALNLC 120

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFV+TEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVETEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240
           KIPSSLLNIFKELR DLGCSIPSHGNLEKWAVQGVLLLNAVL+VRKHQANSHAK+GWEQF
Sbjct: 181 KIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQF 240

Query: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TD VIKTISQ KEG+VFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDVVIKTISQNKEGVVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNILLKELGTASIDWQL 319
           RTN+LLKELG  +IDWQL
Sbjct: 301 RTNVLLKELGIDAIDWQL 318

BLAST of PI0009172 vs. NCBI nr
Match: XP_008465227.1 (PREDICTED: uracil-DNA glycosylase, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 627.9 bits (1618), Expect = 4.9e-176
Identity = 312/318 (98.11%), Postives = 316/318 (99.37%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT DDKCDSDLTLASSSTDMSASQ
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSTDMSASQ 60

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
           ISRMETNKWMARSKRNLKICSDRVSKWENGC+KLEELLVEETWFEALPGEFEKPYALNLC
Sbjct: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCMKLEELLVEETWFEALPGEFEKPYALNLC 120

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240
           KIPSSLLNIFKEL++DLGCSIPSHGNL KWAVQGVLLLNAVLSVR+HQANSHAKRGWEQF
Sbjct: 181 KIPSSLLNIFKELKDDLGCSIPSHGNLGKWAVQGVLLLNAVLSVREHQANSHAKRGWEQF 240

Query: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNILLKELGTASIDWQL 319
           RTNILLKELGTASIDWQL
Sbjct: 301 RTNILLKELGTASIDWQL 318

BLAST of PI0009172 vs. NCBI nr
Match: XP_004140430.1 (uracil-DNA glycosylase, mitochondrial [Cucumis sativus] >KGN50850.1 hypothetical protein Csa_004717 [Cucumis sativus])

HSP 1 Score: 622.5 bits (1604), Expect = 2.1e-174
Identity = 308/318 (96.86%), Postives = 314/318 (98.74%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT DDKCDSDLTLASSS D+SASQ
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSADISASQ 60

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
           ISRMETNKW+ARSKRNLK CSDRVSKWENGCVKLEELLVEETWFEALPGEF+KPYALNLC
Sbjct: 61  ISRMETNKWIARSKRNLKTCSDRVSKWENGCVKLEELLVEETWFEALPGEFQKPYALNLC 120

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240
           KIPSSLLNIFKELR+DLGCSIPSHGNL KWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF
Sbjct: 181 KIPSSLLNIFKELRDDLGCSIPSHGNLGKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240

Query: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVIKTISQKKEGI+FLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDAVIKTISQKKEGIIFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNILLKELGTASIDWQL 319
           RTNILLKE+GTASIDWQL
Sbjct: 301 RTNILLKEMGTASIDWQL 318

BLAST of PI0009172 vs. NCBI nr
Match: XP_008465225.1 (PREDICTED: uracil-DNA glycosylase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 614.0 bits (1582), Expect = 7.3e-172
Identity = 312/343 (90.96%), Postives = 316/343 (92.13%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLAT DDKCDSDLTLASSSTDMSASQ
Sbjct: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATNDDKCDSDLTLASSSTDMSASQ 60

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
           ISRMETNKWMARSKRNLKICSDRVSKWENGC+KLEELLVEETWFEALPGEFEKPYALNLC
Sbjct: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCMKLEELLVEETWFEALPGEFEKPYALNLC 120

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLS----------------- 240
           KIPSSLLNIFKEL++DLGCSIPSHGNL KWAVQGVLLLNAVLS                 
Sbjct: 181 KIPSSLLNIFKELKDDLGCSIPSHGNLGKWAVQGVLLLNAVLSATSRILNQKLFKHHQTV 240

Query: 241 --------VRKHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKK 300
                   VR+HQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKK
Sbjct: 241 MKSNKADIVREHQANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKK 300

Query: 301 HHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL 319
           HHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL
Sbjct: 301 HHILKAAHPSGLSANRGFFGCRHFSRTNILLKELGTASIDWQL 343

BLAST of PI0009172 vs. NCBI nr
Match: XP_038891400.1 (uracil-DNA glycosylase, mitochondrial [Benincasa hispida])

HSP 1 Score: 611.3 bits (1575), Expect = 4.7e-171
Identity = 302/318 (94.97%), Postives = 313/318 (98.43%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTD+KCDS+LTLAS S DMSA+Q
Sbjct: 46  MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDEKCDSELTLASFSIDMSAAQ 105

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
            SRMETNKWMARSKRNLKI SDRVSKWENGC+KLE+LLVEETWFEALPGEFEKPYA+NLC
Sbjct: 106 KSRMETNKWMARSKRNLKIVSDRVSKWENGCMKLEDLLVEETWFEALPGEFEKPYAVNLC 165

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 166 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 225

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240
           KIPSSLLNIFKEL++DLGCSIPSHGNLEKWAVQGVLLLN VLSVRKHQANSHAK+GWEQF
Sbjct: 226 KIPSSLLNIFKELKDDLGCSIPSHGNLEKWAVQGVLLLNTVLSVRKHQANSHAKKGWEQF 285

Query: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSA+RGFFGCRHFS
Sbjct: 286 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSASRGFFGCRHFS 345

Query: 301 RTNILLKELGTASIDWQL 319
           RTN+LLKELGTASIDWQL
Sbjct: 346 RTNVLLKELGTASIDWQL 363

BLAST of PI0009172 vs. NCBI nr
Match: XP_023525611.1 (uracil-DNA glycosylase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602.8 bits (1553), Expect = 1.7e-168
Identity = 296/318 (93.08%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MASSSASLSSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSDLTLASSSTDMSASQ 60
           MAS S SL SKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDS+LTLASSS D+S+SQ
Sbjct: 1   MASFSPSLKSKTRTLIDIFQPALSKRLKTSQTLKTLATTDDKCDSELTLASSSMDISSSQ 60

Query: 61  ISRMETNKWMARSKRNLKICSDRVSKWENGCVKLEELLVEETWFEALPGEFEKPYALNLC 120
            SRMETNKW+ARSKRNLKI SDRVSKWENGCVKLEELLV+ETWFEALPGEFEKPYALNLC
Sbjct: 61  KSRMETNKWLARSKRNLKISSDRVSKWENGCVKLEELLVDETWFEALPGEFEKPYALNLC 120

Query: 121 KFVQTEICSSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180
           KFV+TEICSSGVP+YPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV
Sbjct: 121 KFVETEICSSGVPVYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQAMGLSFSVPEGV 180

Query: 181 KIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQANSHAKRGWEQF 240
           KIPSSLLNIFKELR DLGCSIPSHGNLEKWAVQGVLLLNAVL+VRKHQANSHAK+GWEQF
Sbjct: 181 KIPSSLLNIFKELREDLGCSIPSHGNLEKWAVQGVLLLNAVLTVRKHQANSHAKKGWEQF 240

Query: 241 TDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300
           TDAVI+TISQKKEG+VFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS
Sbjct: 241 TDAVIQTISQKKEGVVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLSANRGFFGCRHFS 300

Query: 301 RTNILLKELGTASIDWQL 319
           RTN+LLKELG  +IDWQL
Sbjct: 301 RTNMLLKELGIGAIDWQL 318

BLAST of PI0009172 vs. TAIR 10
Match: AT3G18630.1 (uracil dna glycosylase )

HSP 1 Score: 399.4 bits (1025), Expect = 2.6e-111
Identity = 209/330 (63.33%), Postives = 247/330 (74.85%), Query Frame = 0

Query: 9   SSKTRTLIDIFQPALSKRLKT---------------SQTLKTLATTDDKCDSDLTLASSS 68
           SS  +TL+D FQPA  KRLK                S+ L ++A +  +     ++A  S
Sbjct: 3   SSTPKTLMDFFQPA--KRLKASPSSSSFPAVSVAGGSRDLGSVANSPPRVTVTTSVADDS 62

Query: 69  TDMSASQISRMETNKWMARSKRNLKICSDRV--SKWENGC-VKLEELLVEETWFEALPGE 128
           + ++  QI+R E NK++A+SKRNL +CS+RV  +K E  C V L ELLVEE+W +ALPGE
Sbjct: 63  SGLTPEQIARAEFNKFVAKSKRNLAVCSERVTKAKSEGNCYVPLSELLVEESWLKALPGE 122

Query: 129 FEKPYALNLCKFVQTEIC--SSGVPIYPPPSLIFNALNSTPFDRVKVVILGQDPYHGPGQ 188
           F KPYA +L  F++ EI   S    IYPP  LIFNALN+TPFDRVK VI+GQDPYHGPGQ
Sbjct: 123 FHKPYAKSLSDFLEREIITDSKSPLIYPPQHLIFNALNTTPFDRVKTVIIGQDPYHGPGQ 182

Query: 189 AMGLSFSVPEGVKIPSSLLNIFKELRNDLGCSIPSHGNLEKWAVQGVLLLNAVLSVRKHQ 248
           AMGLSFSVPEG K+PSSLLNIFKEL  D+GCSIP HGNL+KWAVQGVLLLNAVL+VR  Q
Sbjct: 183 AMGLSFSVPEGEKLPSSLLNIFKELHKDVGCSIPRHGNLQKWAVQGVLLLNAVLTVRSKQ 242

Query: 249 ANSHAKRGWEQFTDAVIKTISQKKEGIVFLLWGNSAQAKLRLIDEKKHHILKAAHPSGLS 308
            NSHAK+GWEQFTDAVI++ISQ+KEG+VFLLWG  AQ K +LID  KHHIL AAHPSGLS
Sbjct: 243 PNSHAKKGWEQFTDAVIQSISQQKEGVVFLLWGRYAQEKSKLIDATKHHILTAAHPSGLS 302

Query: 309 ANRGFFGCRHFSRTNILLKELGTASIDWQL 319
           ANRGFF CRHFSR N LL+E+G   IDWQL
Sbjct: 303 ANRGFFDCRHFSRANQLLEEMGIPPIDWQL 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIH63.7e-11063.33Uracil-DNA glycosylase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=UNG PE=... [more]
C1DQR01.4e-7258.48Uracil-DNA glycosylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) OX=... [more]
A7I0A88.9e-7260.79Uracil-DNA glycosylase OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568... [more]
Q88N051.5e-7157.59Uracil-DNA glycosylase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCI... [more]
A5W8H22.6e-7157.59Uracil-DNA glycosylase OS=Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BC... [more]
Match NameE-valueIdentityDescription
A0A1S3CPW32.4e-17698.11Uracil-DNA glycosylase OS=Cucumis melo OX=3656 GN=LOC103502882 PE=3 SV=1[more]
A0A0A0KMG39.9e-17596.86Uracil-DNA glycosylase OS=Cucumis sativus OX=3659 GN=Csa_5G289610 PE=3 SV=1[more]
A0A1S3CND73.5e-17290.96Uracil-DNA glycosylase OS=Cucumis melo OX=3656 GN=LOC103502882 PE=3 SV=1[more]
A0A6J1J2E42.4e-16892.77Uracil-DNA glycosylase OS=Cucurbita maxima OX=3661 GN=LOC111480636 PE=3 SV=1[more]
A0A6J1EEH83.4e-16792.45Uracil-DNA glycosylase OS=Cucurbita moschata OX=3662 GN=LOC111433476 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008465227.14.9e-17698.11PREDICTED: uracil-DNA glycosylase, mitochondrial isoform X2 [Cucumis melo][more]
XP_004140430.12.1e-17496.86uracil-DNA glycosylase, mitochondrial [Cucumis sativus] >KGN50850.1 hypothetical... [more]
XP_008465225.17.3e-17290.96PREDICTED: uracil-DNA glycosylase, mitochondrial isoform X1 [Cucumis melo][more]
XP_038891400.14.7e-17194.97uracil-DNA glycosylase, mitochondrial [Benincasa hispida][more]
XP_023525611.11.7e-16893.08uracil-DNA glycosylase, mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G18630.12.6e-11163.33uracil dna glycosylase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005122Uracil-DNA glycosylase-likeSMARTSM00986UDG_2coord: 146..306
e-value: 4.9E-39
score: 145.7
IPR005122Uracil-DNA glycosylase-likePFAMPF03167UDGcoord: 151..307
e-value: 1.7E-24
score: 86.6
NoneNo IPR availableSMARTSM00987UDG_2_acoord: 146..306
e-value: 4.9E-39
score: 145.7
IPR036895Uracil-DNA glycosylase-like domain superfamilyGENE3D3.40.470.10coord: 80..318
e-value: 1.6E-97
score: 327.8
IPR036895Uracil-DNA glycosylase-like domain superfamilySUPERFAMILY52141Uracil-DNA glycosylase-likecoord: 98..318
IPR002043Uracil-DNA glycosylase family 1TIGRFAMTIGR00628TIGR00628coord: 101..308
e-value: 4.6E-79
score: 263.1
IPR002043Uracil-DNA glycosylase family 1PANTHERPTHR11264URACIL-DNA GLYCOSYLASEcoord: 34..318
IPR002043Uracil-DNA glycosylase family 1HAMAPMF_00148UDGcoord: 100..317
score: 38.680672
IPR002043Uracil-DNA glycosylase family 1CDDcd10027UDG-F1-likecoord: 115..316
e-value: 1.80028E-130
score: 367.93
IPR018085Uracil-DNA glycosylase, active sitePROSITEPS00130U_DNA_GLYCOSYLASEcoord: 154..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0009172.1PI0009172.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0097510 base-excision repair, AP site formation via deaminated base removal
biological_process GO:0006284 base-excision repair
biological_process GO:0006281 DNA repair
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
molecular_function GO:0004844 uracil DNA N-glycosylase activity
molecular_function GO:0016799 hydrolase activity, hydrolyzing N-glycosyl compounds