PI0008564 (gene) Melon (PI 482460) v1

Overview
NamePI0008564
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionNuclear ribonuclease Z
Locationchr08: 4960345 .. 4962996 (+)
RNA-Seq ExpressionPI0008564
SyntenyPI0008564
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCCGGAACTGCCAACTTTAGCAGCAAAATCTCGGACTCGAACCCGATGGAAATGAGGAAGGGAATACAAATTGAAGGATACCCGATTAAGGGCTTGTCCATTGGAGGCCATGAAACTTGCGTGATTTTCCCAACTCTCAATTTGGCCTTTGATATTGGTCGCTGCCCTCAAAAGGCCATAAGCCAGGACTTCCTCTTCATCTCCCATGGTCATATGGATCACATTGTAAGTCCAGGCCCCTCTCTCTGCTTCATTTTTCAACCATTTTGTTATCGTCTGAGCTTGTTCCCATGGCGGGAGACCTCACTGTTGATACGTTTAGTTGATGTTGAAGTGGAATTTCATTGATTTAGGGTTTAGGGTTTGAAGTCTAGTAATTTCCACTGGTTCTGTTGCAGCTATCTTCAATCAATTCAAGTATCTATTTAATATTTGAATTTCGTTTTACCTTTCCAATTCAAACAGCTATTTGAATGTAAGCCTTTTTGCCGTTTGTTCATCTGTAGCTCATCAGTAGCTTTTGTGCTTAATAGGGGGGACTTCCCTTGTACGTTGCTACCCGTGGATTATACAAAATGGGACCACCAACAATTGTTGTACCAAAATCTATAAAGAATGATGTAGAAAATCTTTTAGAGGTGCACAGAAGATTGGATCAATCTGAGCTGAGATGTAATTTGATTGGCTTGGAAGTGGGTATCGTCTCTTATTGAATCATCTTATTTTGTCCAATGAATAGTTTGGTTCCCAATTTTCGTGCTTTGGTTTAATACATTCTTGCATTCATATATTTTGTTTGCAGGGGAAGAGTTGTCATTGAGAAGGGACCTCAAAGTGAGGGTGTTTAGGACATACCATGTCATTGACAGCCAGGTAGCCTACTTCTTGCACATAAACTTTGATTTTATTATTATTCTTAGGTTTATTATTATCCTTTTTGAGTTCTACAATATGTGGGTAGGAGATTTGAACTTCTGAGCTCTTGGTCAAAAGTATATACTTTTTGCAGGTTGCTATTTTTATGTTGCCATTTTTCATGATTTTGTTCTTCTTGTATGTCCAAAATTTACCTATAACTATGGCAGGGTTATCTACTTTACTCTGTAAAGCAGAAGCTTAAGAAAGATTACTTGGGACTATCTGGCAACGAGATCAAGAAACTCCGCTTATCTGGTGTAGAGGTTTGTCTTAACCATTAGATTCACAGATTTCTTTGTAATGGAACACATGGACTTTGAAAAACTTCATTTTTATACATTCCATCATTGAAAAAGGGTTTAGTGGCTACCCTGGCGAATAATTAGACTAGGAATTTGTGAGTATTATGGCAGGTAGGTGACTTAAAGCATTTGAAACAACAGTCATTTGGTTGAAATCAGGGTCTTTTTCACTATATTTCCTTAAATTTCAACCTTATTTCTTAGTTAAAGTGAACAAAGTTTAACGCCTCTCTGATTATACTTTTTCTTTTACATTTCCCCATTCTTTAAACTTCTGCTCAAGTACACAATAATCTTTGGTTATTTTTCTTTGTGCCAGATTACTTACACAATTACCGAGCCTGAAGTGGCTTTTACAGGAGATACCACGTCCGACTTTATAGTTGATGAAAATAATATTGACGTATTGAGGGCGAAGATCCTGGTTATGGAGGTCCGGTACTAGAAGGCTACACACTATGTCTCAATCTTGTTTTCCTCAAACGAATGTTTGATTATTATTACTTTTGGTTAACCTTTTGAACAGAAATAGAGTTTTGCAGAGTGAGTTTGAATGATAAATAGCTTCTTTTAAGACAGCATGATGTATATTTATGAATGCGTTGTAGCTTAATAAAATGAAATTAGCATTAGAATGCAGATTTTTTTCCTACATATGGGGTTCTGCATGGTTACGGATTGGTATAAGATATGTTTGCAAGTAATTTTGACATGATTAAACTTATGCTAAAAATCACTTAGAAACATGCATCTACTCAATAAAAAACAATTTGATGTTTGATTTTATACTTTTGAACATGCCCATAGGTTTAGATTGGAAAGAGAAAATGGTGTTGGTTATCACTTTGGTTGGGTGGGTTCTACTTCTATTGAGGTTTAATCCTTCATGGTGCACAACCATTCTTTGCAGAGCACATTTCTTGAAAATAGAGTTACAGTGGAGCATGCTAGAGAATACGGACACACTCACTTATTTGAGGTTCGTTTACTCAACATTGTAATACATGTTATTCCTGTATTCATTCAGCAACTTATATCTTATAATTTTACATCCTTGTGTGCTTTGTATGTTGATGATACAACCCAGATAATAAATCATGCGAAAAAGTTTAAGAACAAAGCGATTCTTCTGATTCACTTTTCAGCTCGCTACACTACAGAGGTACGTCTAATACCAGTTTATTGATCTCTTAAGTTCATGTTTTTATCGAGTTTAATAGTGCAATGCTTCTATTAGGTGACATCCTCTTCTTTCCACTTCCATCTCCTTTATAGAGAGGATTGAGAAACATTTTATTTTCCAGTATGTATTGGTAAAGCTAAATACATTTCCTGCTTTTTGGTTTATCAGGAAATTCAGCAAGCCATATCTACTTTACCTCCAACTTTGGCGGGCCGAGTTTCTGCGCTAACGGAAGGTTTTTGA

mRNA sequence

ATGGCAGCCGGAACTGCCAACTTTAGCAGCAAAATCTCGGACTCGAACCCGATGGAAATGAGGAAGGGAATACAAATTGAAGGATACCCGATTAAGGGCTTGTCCATTGGAGGCCATGAAACTTGCGTGATTTTCCCAACTCTCAATTTGGCCTTTGATATTGGTCGCTGCCCTCAAAAGGCCATAAGCCAGGACTTCCTCTTCATCTCCCATGGTCATATGGATCACATTGGGGGACTTCCCTTGTACGTTGCTACCCGTGGATTATACAAAATGGGACCACCAACAATTGTTGTACCAAAATCTATAAAGAATGATGTAGAAAATCTTTTAGAGGTGCACAGAAGATTGGATCAATCTGAGCTGAGATGTAATTTGATTGGCTTGGAAGTGGGGGAAGAGTTGTCATTGAGAAGGGACCTCAAAGTGAGGGTGTTTAGGACATACCATGTCATTGACAGCCAGGGTTATCTACTTTACTCTGTAAAGCAGAAGCTTAAGAAAGATTACTTGGGACTATCTGGCAACGAGATCAAGAAACTCCGCTTATCTGGTGTAGAGATTACTTACACAATTACCGAGCCTGAAGTGGCTTTTACAGGAGATACCACGTCCGACTTTATAGTTGATGAAAATAATATTGACGTATTGAGGGCGAAGATCCTGGTTATGGAGAGCACATTTCTTGAAAATAGAGTTACAGTGGAGCATGCTAGAGAATACGGACACACTCACTTATTTGAGATAATAAATCATGCGAAAAAGTTTAAGAACAAAGCGATTCTTCTGATTCACTTTTCAGCTCGCTACACTACAGAGGAAATTCAGCAAGCCATATCTACTTTACCTCCAACTTTGGCGGGCCGAGTTTCTGCGCTAACGGAAGGTTTTTGA

Coding sequence (CDS)

ATGGCAGCCGGAACTGCCAACTTTAGCAGCAAAATCTCGGACTCGAACCCGATGGAAATGAGGAAGGGAATACAAATTGAAGGATACCCGATTAAGGGCTTGTCCATTGGAGGCCATGAAACTTGCGTGATTTTCCCAACTCTCAATTTGGCCTTTGATATTGGTCGCTGCCCTCAAAAGGCCATAAGCCAGGACTTCCTCTTCATCTCCCATGGTCATATGGATCACATTGGGGGACTTCCCTTGTACGTTGCTACCCGTGGATTATACAAAATGGGACCACCAACAATTGTTGTACCAAAATCTATAAAGAATGATGTAGAAAATCTTTTAGAGGTGCACAGAAGATTGGATCAATCTGAGCTGAGATGTAATTTGATTGGCTTGGAAGTGGGGGAAGAGTTGTCATTGAGAAGGGACCTCAAAGTGAGGGTGTTTAGGACATACCATGTCATTGACAGCCAGGGTTATCTACTTTACTCTGTAAAGCAGAAGCTTAAGAAAGATTACTTGGGACTATCTGGCAACGAGATCAAGAAACTCCGCTTATCTGGTGTAGAGATTACTTACACAATTACCGAGCCTGAAGTGGCTTTTACAGGAGATACCACGTCCGACTTTATAGTTGATGAAAATAATATTGACGTATTGAGGGCGAAGATCCTGGTTATGGAGAGCACATTTCTTGAAAATAGAGTTACAGTGGAGCATGCTAGAGAATACGGACACACTCACTTATTTGAGATAATAAATCATGCGAAAAAGTTTAAGAACAAAGCGATTCTTCTGATTCACTTTTCAGCTCGCTACACTACAGAGGAAATTCAGCAAGCCATATCTACTTTACCTCCAACTTTGGCGGGCCGAGTTTCTGCGCTAACGGAAGGTTTTTGA

Protein sequence

MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKKLRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF
Homology
BLAST of PI0008564 vs. ExPASy Swiss-Prot
Match: Q8LGU7 (tRNase Z TRZ1 OS=Arabidopsis thaliana OX=3702 GN=TRZ1 PE=1 SV=3)

HSP 1 Score: 436.4 bits (1121), Expect = 2.6e-121
Identity = 204/280 (72.86%), Postives = 246/280 (87.86%), Query Frame = 0

Query: 18  MEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAISQDFLFISHGHMDHI 77
           ME +K +QIEGYPI+GLSIGGHETC+IFP+L +AFDIGRCP +AISQDFLFISH HMDHI
Sbjct: 1   MEKKKAMQIEGYPIEGLSIGGHETCIIFPSLRIAFDIGRCPHRAISQDFLFISHSHMDHI 60

Query: 78  GGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEELSL 137
           GGLP+YVATRGLYKM PPTI+VP SIK  VE+L EVHR+LD SEL+ NL+GL++GEE  +
Sbjct: 61  GGLPMYVATRGLYKMKPPTIIVPASIKETVESLFEVHRKLDSSELKHNLVGLDIGEEFII 120

Query: 138 RRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKKLRLSGVEITYTITEPEV 197
           R+DLKV+ F+T+HVI SQGY++YS K KLKK+Y+GLSGNEIK L++SGVEIT +I  PEV
Sbjct: 121 RKDLKVKAFKTFHVIQSQGYVVYSTKYKLKKEYIGLSGNEIKNLKVSGVEITDSIITPEV 180

Query: 198 AFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAKKFK 257
           AFTGDTTSDF+VDE N D L+AK+LVMESTFL++ V+VEHAR+YGH H+ EI+NHA+KF+
Sbjct: 181 AFTGDTTSDFVVDETNADALKAKVLVMESTFLDDSVSVEHARDYGHIHISEIVNHAEKFE 240

Query: 258 NKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           NKAILLIHFSARYT +EI+ A+S LPP L GRV ALT+GF
Sbjct: 241 NKAILLIHFSARYTVKEIEDAVSALPPPLEGRVFALTQGF 280

BLAST of PI0008564 vs. ExPASy Swiss-Prot
Match: Q8L633 (tRNase Z TRZ2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TRZ2 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 3.6e-91
Identity = 158/277 (57.04%), Postives = 207/277 (74.73%), Query Frame = 0

Query: 21  RKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAISQDFLFISHGHMDHIGGL 80
           RKG+++E Y I+G+S+GGHETCVI P L   FDIGRCP +AI Q FLFI+H H+DHIGGL
Sbjct: 73  RKGVELESYAIEGISVGGHETCVIVPELKCVFDIGRCPSRAIQQKFLFITHAHLDHIGGL 132

Query: 81  PLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEELSLRRD 140
           P+YVA+RGLY + PP I VP SIK DVE LLE+HR + Q EL   LI L VGE   LR D
Sbjct: 133 PMYVASRGLYNLEPPKIFVPPSIKEDVEKLLEIHRTMGQVELNVELIPLAVGETYELRND 192

Query: 141 LKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKKLRLSGVEITYTITEPEVAFT 200
           + VR F T+HVI SQGY++YSV++KL+K Y  L G +I+K++ SGVEIT TI  PE+AFT
Sbjct: 193 IVVRPFATHHVIPSQGYVIYSVRKKLQKQYAHLKGKQIEKIKKSGVEITDTILSPEIAFT 252

Query: 201 GDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAKKFKNKA 260
           GDTTS++++D  N D LRAK+L+ E+TFL+   + EHA+  GHTH+ +II +AK  ++K 
Sbjct: 253 GDTTSEYMLDPRNADALRAKVLITEATFLDESFSTEHAQALGHTHISQIIENAKWIRSKT 312

Query: 261 ILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           +LL HFS+RY  EEI++A+  L   ++ +V  LTEGF
Sbjct: 313 VLLTHFSSRYHVEEIREAVLKLQSKVSAKVIPLTEGF 349

BLAST of PI0008564 vs. ExPASy Swiss-Prot
Match: P60193 (Nuclear ribonuclease Z (Fragment) OS=Triticum aestivum OX=4565 GN=ELAC PE=1 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.4e-71
Identity = 122/170 (71.76%), Postives = 150/170 (88.24%), Query Frame = 0

Query: 24  IQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAISQDFLFISHGHMDHIGGLPLY 83
           ++IEGYP++G+SIGGHETCVIFPTL+LAFDIGRCPQ+A++QDFLFISH H+DHIGGLP+Y
Sbjct: 7   LEIEGYPVEGISIGGHETCVIFPTLSLAFDIGRCPQRAVAQDFLFISHAHLDHIGGLPMY 66

Query: 84  VATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEELSLRRDLKV 143
           VATRGLY++ PPTI VPK ++  VE L +VHR +DQSEL   L+ L++GEE  LRRDLKV
Sbjct: 67  VATRGLYRLRPPTIFVPKYLRELVERLFDVHRAMDQSELNHTLVPLDIGEEYELRRDLKV 126

Query: 144 RVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKKLRLSGVEITYTIT 194
           R F+TYH I SQGY++YSVKQKLK+DYLGL G+EIK+L+LSGVEIT T+T
Sbjct: 127 RAFKTYHTIPSQGYVIYSVKQKLKQDYLGLPGSEIKRLKLSGVEITNTVT 176

BLAST of PI0008564 vs. ExPASy Swiss-Prot
Match: Q973F1 (Ribonuclease Z OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=rnz PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 4.8e-11
Identity = 63/221 (28.51%), Postives = 105/221 (47.51%), Query Frame = 0

Query: 69  ISHGHMDHIGGLPLYVATRGLYKMGPPTIVV-PKSIKNDVENLLEVHRRLDQSELRCNLI 128
           I+H H DH+ GLP  + T G+Y      +++ PK +K  +E++ +        E++    
Sbjct: 58  ITHMHGDHVLGLPGMIETMGMYSRKESLLLMGPKELKEFLEDIFKKTYFYPNFEIQI--- 117

Query: 129 GLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKKLRL---- 188
            ++  E+     ++K+  F T H I+SQGY L+  K +LK D   L    IK  R+    
Sbjct: 118 -IDKYED----ENIKISTFETCHTIESQGY-LFEEKDRLKIDIDKLRKEGIKDWRIIRML 177

Query: 189 ---SGVEITYTITEPE----------VAFTGDTTSDFIVDENNIDVLR-AKILVMESTFL 248
                VEI   +  PE          +A+TGDT       E  I+ ++   +L+ +STF+
Sbjct: 178 KEGKRVEINGKVLLPEDYLIVKKGIRIAYTGDTGP----CEKVINAVKDVDLLIHDSTFI 237

Query: 249 ENRVTVEHAREYGHTHLFEIINHAKKFKNKAILLIHFSARY 271
           + +     A +YGH++ ++    A K   K + L H S RY
Sbjct: 238 DEK----EAYKYGHSNSYDAAYVALKANVKRLALFHISPRY 261

BLAST of PI0008564 vs. ExPASy Swiss-Prot
Match: Q49XV1 (Ribonuclease Z OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) OX=342451 GN=rnz PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.4e-10
Identity = 71/268 (26.49%), Postives = 112/268 (41.79%), Query Frame = 0

Query: 52  FDIGRCPQKAI--------SQDFLFISHGHMDHIGGLPLYVATRGLY--KMGPPTIVVPK 111
           FD+G   Q  I          D +FI+H H DHI GLP  + +R     +  P T++ PK
Sbjct: 36  FDVGEGTQHQILHHSIKLGKVDHIFITHMHGDHIFGLPGLLTSRSFQGGEGKPLTLIGPK 95

Query: 112 SIKNDVENLLEVHRRLDQSELRCNLIGLEVGEELSLRRD-LKVRVFRTYHVIDSQGYLLY 171
            IK  +E  L    RL +S+L   +  +E+   LS + +   V      H I S GY + 
Sbjct: 96  GIKAYIETTL----RLSESKLNYPITYIEIENHLSYQNNGFNVEAHMLNHGIPSFGYRIE 155

Query: 172 SVKQKLKKD--------------YLGLSGNEIKKLRLSGVEITY------TITEPEVAFT 231
           +     K D              Y  +  NE      +G+           +  P +A  
Sbjct: 156 APYTSGKIDVSALKEIGLEPGPKYQDVKNNE--TFEYNGIIYNSKDFKGDAVKGPVIAIF 215

Query: 232 GDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAKKFKNKA 286
           GDT          I    A ++V EST++E   ++  A  Y H+H+ ++    +      
Sbjct: 216 GDTMP---CQNEEIIADHANVMVHESTYIEGDKSL--ANNYHHSHIEDVFTLIENAHVDY 275

BLAST of PI0008564 vs. ExPASy TrEMBL
Match: A0A1S3C5G3 (nuclear ribonuclease Z OS=Cucumis melo OX=3656 GN=LOC103497261 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.7e-160
Identity = 290/297 (97.64%), Postives = 291/297 (97.98%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           MAAGTANFSSK  DSNPMEM KGIQI GYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQDFLFISHGHMDHIGGLPLYVATRGLYKM PPTIVVPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQ+AISTLPP LAGRVSALTEGF
Sbjct: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQEAISTLPPLLAGRVSALTEGF 297

BLAST of PI0008564 vs. ExPASy TrEMBL
Match: A0A5A7V6W7 (Nuclear ribonuclease Z OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00700 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.7e-160
Identity = 290/297 (97.64%), Postives = 291/297 (97.98%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           MAAGTANFSSK  DSNPMEM KGIQI GYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQDFLFISHGHMDHIGGLPLYVATRGLYKM PPTIVVPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQ+AISTLPP LAGRVSALTEGF
Sbjct: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQEAISTLPPPLAGRVSALTEGF 297

BLAST of PI0008564 vs. ExPASy TrEMBL
Match: A0A5D3DXQ4 (Nuclear ribonuclease Z OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold258G00190 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 4.8e-155
Identity = 290/333 (87.09%), Postives = 291/333 (87.39%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           MAAGTANFSSK  DSNPMEM KGIQI GYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQDFLFISHGHMDHIGGLPLYVATRGLYKM PPTIVVPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFE------------------------------------IINHAKKFKNKAILLI 298
           YGHTHLFE                                    IINHAKKFKNKAILLI
Sbjct: 241 YGHTHLFEVGLLNIVIPVIPVFIQQLISYNFTSLSALYVDDPTQIINHAKKFKNKAILLI 300

BLAST of PI0008564 vs. ExPASy TrEMBL
Match: A0A6J1GGV3 (tRNase Z TRZ1 OS=Cucurbita moschata OX=3662 GN=LOC111453818 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 6.7e-149
Identity = 268/297 (90.24%), Postives = 282/297 (94.95%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           M AGT + SSK  DS+  E  KG+QIEGYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQK
Sbjct: 1   MEAGTGS-SSKSLDSDLKERTKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQK 60

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQ+FL ISHGHMDHIGGLPLYVATRGLYKM PPTI+VPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEE SLRRDLKVR F+TYHVIDSQGYLLYSVKQKLKK+YLGL GNEIKK
Sbjct: 121 ELRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKK 180

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVE+TYTIT+PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           YGHTHLFEIINHA+KFKNKAIL+IHFSARYTTEEIQQA++TLPP LAGRVSALTEGF
Sbjct: 241 YGHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVATLPPPLAGRVSALTEGF 296

BLAST of PI0008564 vs. ExPASy TrEMBL
Match: A0A6J1IPL7 (tRNase Z TRZ1 OS=Cucurbita maxima OX=3661 GN=LOC111478792 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.5e-148
Identity = 267/297 (89.90%), Postives = 281/297 (94.61%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           M AGT + SSK  DS+  E  KG+QIEGYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQK
Sbjct: 1   MEAGTGS-SSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQK 60

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQ+FL ISHGHMDHIGGLPLYVATRGLYKM PPTI+VPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEE SLRRDLKVR F+TYHVIDSQGYLLYSVKQKLKK+YLGL GNEIKK
Sbjct: 121 ELRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKK 180

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVE+TYTIT+PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           YGHTHLFEIINHA+KFKNKAIL+IHFSARYTTEEIQQA++ LPP LAGRVSALTEGF
Sbjct: 241 YGHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 296

BLAST of PI0008564 vs. NCBI nr
Match: XP_008457600.1 (PREDICTED: nuclear ribonuclease Z [Cucumis melo] >XP_008457601.1 PREDICTED: nuclear ribonuclease Z [Cucumis melo] >XP_008457604.1 PREDICTED: nuclear ribonuclease Z [Cucumis melo])

HSP 1 Score: 575.1 bits (1481), Expect = 3.5e-160
Identity = 290/297 (97.64%), Postives = 291/297 (97.98%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           MAAGTANFSSK  DSNPMEM KGIQI GYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQDFLFISHGHMDHIGGLPLYVATRGLYKM PPTIVVPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQ+AISTLPP LAGRVSALTEGF
Sbjct: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQEAISTLPPLLAGRVSALTEGF 297

BLAST of PI0008564 vs. NCBI nr
Match: KAA0063843.1 (nuclear ribonuclease Z [Cucumis melo var. makuwa])

HSP 1 Score: 575.1 bits (1481), Expect = 3.5e-160
Identity = 290/297 (97.64%), Postives = 291/297 (97.98%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           MAAGTANFSSK  DSNPMEM KGIQI GYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQDFLFISHGHMDHIGGLPLYVATRGLYKM PPTIVVPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQ+AISTLPP LAGRVSALTEGF
Sbjct: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQEAISTLPPPLAGRVSALTEGF 297

BLAST of PI0008564 vs. NCBI nr
Match: XP_011653493.2 (LOW QUALITY PROTEIN: tRNase Z TRZ1 [Cucumis sativus])

HSP 1 Score: 568.9 bits (1465), Expect = 2.5e-158
Identity = 285/297 (95.96%), Postives = 289/297 (97.31%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           MAAGTA+FSSK  DSNPMEM KGIQIEGYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTASFSSKNLDSNPMEMSKGIQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQK 60

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQD  FISHGHMDHIGGLPLYVATRGLYKMGPPTI+VPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQDSFFISHGHMDHIGGLPLYVATRGLYKMGPPTIIVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVEITYTITEPEVAFTGDTT DFIVDENNIDVLRAKILVMESTFLENRV VEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTXDFIVDENNIDVLRAKILVMESTFLENRVKVEHARE 240

Query: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQ+AISTLPP LAGRVSALTEGF
Sbjct: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQEAISTLPPPLAGRVSALTEGF 297

BLAST of PI0008564 vs. NCBI nr
Match: TYK28208.1 (nuclear ribonuclease Z [Cucumis melo var. makuwa])

HSP 1 Score: 557.0 bits (1434), Expect = 9.9e-155
Identity = 290/333 (87.09%), Postives = 291/333 (87.39%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           MAAGTANFSSK  DSNPMEM KGIQI GYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQDFLFISHGHMDHIGGLPLYVATRGLYKM PPTIVVPKSIKNDVENLLEVHRRLDQS
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLFE------------------------------------IINHAKKFKNKAILLI 298
           YGHTHLFE                                    IINHAKKFKNKAILLI
Sbjct: 241 YGHTHLFEVGLLNIVIPVIPVFIQQLISYNFTSLSALYVDDPTQIINHAKKFKNKAILLI 300

BLAST of PI0008564 vs. NCBI nr
Match: XP_038882012.1 (tRNase Z TRZ1 isoform X1 [Benincasa hispida])

HSP 1 Score: 547.0 bits (1408), Expect = 1.0e-151
Identity = 275/297 (92.59%), Postives = 285/297 (95.96%), Query Frame = 0

Query: 1   MAAGTANFSSKISDSNPMEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           MAAGT   SSK  DS+PME  KG+QIEGYPIKGLSIGGHETCVIFPTL+LAFDIGRCPQK
Sbjct: 27  MAAGT-GCSSKSLDSDPMESCKGMQIEGYPIKGLSIGGHETCVIFPTLSLAFDIGRCPQK 86

Query: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQS 120
           AISQDFLFISHGHMDHIGGLPLYVATRGLYKM PPTI+VPKSIKNDVENLLEVHRRLDQS
Sbjct: 87  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMKPPTIIVPKSIKNDVENLLEVHRRLDQS 146

Query: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180
           ELRCNLIGLEVGEELSLRRDLKVR F+TYHVIDSQGYLLYSVKQKLKK+YLGLSGNEIKK
Sbjct: 147 ELRCNLIGLEVGEELSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLSGNEIKK 206

Query: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240
           LRLSG EITYTITEPEVAFTGDTTSDFIVDENNID+LRAKILVMESTFLENRVTVEHARE
Sbjct: 207 LRLSGAEITYTITEPEVAFTGDTTSDFIVDENNIDILRAKILVMESTFLENRVTVEHARE 266

Query: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           YGHTHLFEIINHA+KFKNKAILLIHFSARYTTEEIQQAI+T+PP LAGRVSAL EGF
Sbjct: 267 YGHTHLFEIINHAEKFKNKAILLIHFSARYTTEEIQQAIATIPPPLAGRVSALMEGF 322

BLAST of PI0008564 vs. TAIR 10
Match: AT1G74700.1 (tRNAse Z1 )

HSP 1 Score: 436.4 bits (1121), Expect = 1.8e-122
Identity = 204/280 (72.86%), Postives = 246/280 (87.86%), Query Frame = 0

Query: 18  MEMRKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAISQDFLFISHGHMDHI 77
           ME +K +QIEGYPI+GLSIGGHETC+IFP+L +AFDIGRCP +AISQDFLFISH HMDHI
Sbjct: 1   MEKKKAMQIEGYPIEGLSIGGHETCIIFPSLRIAFDIGRCPHRAISQDFLFISHSHMDHI 60

Query: 78  GGLPLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEELSL 137
           GGLP+YVATRGLYKM PPTI+VP SIK  VE+L EVHR+LD SEL+ NL+GL++GEE  +
Sbjct: 61  GGLPMYVATRGLYKMKPPTIIVPASIKETVESLFEVHRKLDSSELKHNLVGLDIGEEFII 120

Query: 138 RRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKKLRLSGVEITYTITEPEV 197
           R+DLKV+ F+T+HVI SQGY++YS K KLKK+Y+GLSGNEIK L++SGVEIT +I  PEV
Sbjct: 121 RKDLKVKAFKTFHVIQSQGYVVYSTKYKLKKEYIGLSGNEIKNLKVSGVEITDSIITPEV 180

Query: 198 AFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAKKFK 257
           AFTGDTTSDF+VDE N D L+AK+LVMESTFL++ V+VEHAR+YGH H+ EI+NHA+KF+
Sbjct: 181 AFTGDTTSDFVVDETNADALKAKVLVMESTFLDDSVSVEHARDYGHIHISEIVNHAEKFE 240

Query: 258 NKAILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           NKAILLIHFSARYT +EI+ A+S LPP L GRV ALT+GF
Sbjct: 241 NKAILLIHFSARYTVKEIEDAVSALPPPLEGRVFALTQGF 280

BLAST of PI0008564 vs. TAIR 10
Match: AT2G04530.1 (Metallo-hydrolase/oxidoreductase superfamily protein )

HSP 1 Score: 336.3 bits (861), Expect = 2.6e-92
Identity = 158/277 (57.04%), Postives = 207/277 (74.73%), Query Frame = 0

Query: 21  RKGIQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAISQDFLFISHGHMDHIGGL 80
           RKG+++E Y I+G+S+GGHETCVI P L   FDIGRCP +AI Q FLFI+H H+DHIGGL
Sbjct: 73  RKGVELESYAIEGISVGGHETCVIVPELKCVFDIGRCPSRAIQQKFLFITHAHLDHIGGL 132

Query: 81  PLYVATRGLYKMGPPTIVVPKSIKNDVENLLEVHRRLDQSELRCNLIGLEVGEELSLRRD 140
           P+YVA+RGLY + PP I VP SIK DVE LLE+HR + Q EL   LI L VGE   LR D
Sbjct: 133 PMYVASRGLYNLEPPKIFVPPSIKEDVEKLLEIHRTMGQVELNVELIPLAVGETYELRND 192

Query: 141 LKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKKLRLSGVEITYTITEPEVAFT 200
           + VR F T+HVI SQGY++YSV++KL+K Y  L G +I+K++ SGVEIT TI  PE+AFT
Sbjct: 193 IVVRPFATHHVIPSQGYVIYSVRKKLQKQYAHLKGKQIEKIKKSGVEITDTILSPEIAFT 252

Query: 201 GDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREYGHTHLFEIINHAKKFKNKA 260
           GDTTS++++D  N D LRAK+L+ E+TFL+   + EHA+  GHTH+ +II +AK  ++K 
Sbjct: 253 GDTTSEYMLDPRNADALRAKVLITEATFLDESFSTEHAQALGHTHISQIIENAKWIRSKT 312

Query: 261 ILLIHFSARYTTEEIQQAISTLPPTLAGRVSALTEGF 298
           +LL HFS+RY  EEI++A+  L   ++ +V  LTEGF
Sbjct: 313 VLLTHFSSRYHVEEIREAVLKLQSKVSAKVIPLTEGF 349

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LGU72.6e-12172.86tRNase Z TRZ1 OS=Arabidopsis thaliana OX=3702 GN=TRZ1 PE=1 SV=3[more]
Q8L6333.6e-9157.04tRNase Z TRZ2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TRZ2 PE=2 SV=1[more]
P601932.4e-7171.76Nuclear ribonuclease Z (Fragment) OS=Triticum aestivum OX=4565 GN=ELAC PE=1 SV=1[more]
Q973F14.8e-1128.51Ribonuclease Z OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 1... [more]
Q49XV11.4e-1026.49Ribonuclease Z OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC... [more]
Match NameE-valueIdentityDescription
A0A1S3C5G31.7e-16097.64nuclear ribonuclease Z OS=Cucumis melo OX=3656 GN=LOC103497261 PE=4 SV=1[more]
A0A5A7V6W71.7e-16097.64Nuclear ribonuclease Z OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A5D3DXQ44.8e-15587.09Nuclear ribonuclease Z OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A6J1GGV36.7e-14990.24tRNase Z TRZ1 OS=Cucurbita moschata OX=3662 GN=LOC111453818 PE=4 SV=1[more]
A0A6J1IPL72.5e-14889.90tRNase Z TRZ1 OS=Cucurbita maxima OX=3661 GN=LOC111478792 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008457600.13.5e-16097.64PREDICTED: nuclear ribonuclease Z [Cucumis melo] >XP_008457601.1 PREDICTED: nucl... [more]
KAA0063843.13.5e-16097.64nuclear ribonuclease Z [Cucumis melo var. makuwa][more]
XP_011653493.22.5e-15895.96LOW QUALITY PROTEIN: tRNase Z TRZ1 [Cucumis sativus][more]
TYK28208.19.9e-15587.09nuclear ribonuclease Z [Cucumis melo var. makuwa][more]
XP_038882012.11.0e-15192.59tRNase Z TRZ1 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G74700.11.8e-12272.86tRNAse Z1 [more]
AT2G04530.12.6e-9257.04Metallo-hydrolase/oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001279Metallo-beta-lactamasePFAMPF12706Lactamase_B_2coord: 65..266
e-value: 4.8E-10
score: 39.3
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeGENE3D3.60.15.10coord: 30..289
e-value: 7.6E-45
score: 155.2
IPR036866Ribonuclease Z/Hydroxyacylglutathione hydrolase-likeSUPERFAMILY56281Metallo-hydrolase/oxidoreductasecoord: 45..277
NoneNo IPR availablePANTHERPTHR46504FAMILY NOT NAMEDcoord: 16..297
NoneNo IPR availableCDDcd16272RNaseZ_MBL-foldcoord: 34..226
e-value: 5.21119E-27
score: 101.956

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0008564.1PI0008564.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034414 tRNA 3'-trailer cleavage, endonucleolytic
molecular_function GO:0042781 3'-tRNA processing endoribonuclease activity