PI0008466 (gene) Melon (PI 482460) v1

Overview
NamePI0008466
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionCysteine proteinase inhibitor 5-like
Locationchr08: 7728604 .. 7729394 (+)
RNA-Seq ExpressionPI0008466
SyntenyPI0008466
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCAAGCTTCTTCAAACCATTGCCAAACAAAACTTAAGATGAGTGATATGTTAGTCTCTCCTCGAGGAGCTAATTTGTTGGTTCAAAATCACAAGAATAGTGAATGGAAAACAATAGAAGACACAAAGGACCCCTATGTTGAAGATCTGGCAAGGTTTTCAGTGGCAAAGTTTACACACGATCAGCACATCCAAGAAATGGTGACGTTCATTCGAGTGGTGAGTAGTGAGTGTCTAGTGATGAATGAAGGAACTATGTATCGATTGGTGGTAGAGAGTAGAAAAGAGAATGTAACTCAATCACGTGAGATATATGTGGCTAAGGTATTAGATCATAAGCATCCTTTCAATTCTTGGACACTCTTATCTTTTGAATGCTCTACCCTTTTAGCTTTGCCTTCTAGCTTGGCGCTCTAGAGAATCCTCCCATTTGGTTCATTATCTCTATCCTTTTCAATATGTGTAAATAAAATAAAAGACTTGTATATCTCTAATGCTATGTTTCTTCCTTCTTCATTATCATCCCAACCTTGTGAAAGAGTTATCAACATGCTAGCGAAAGCAATTAGAATCTAAAACTCTTTAAATGTATGAAATTGAATGATTAAGTATGCTTGTATTTAGGCAAAATTGATGAACTTACATTGGTGTAGCTTGAAATTCAAGCAATATTTTGATCAAGAACACTTTGTAGACCACTGCTAGTGTTTTTTTCCCTCTATTCTCTAGCATTAGAACAGTTTGTGGGAGCGAAATCTGTGATGAACTTGAAGTTATTTTGAAGATTTTGG

mRNA sequence

TCTCAAGCTTCTTCAAACCATTGCCAAACAAAACTTAAGATGAGTGATATGTTAGTCTCTCCTCGAGGAGCTAATTTGTTGGTTCAAAATCACAAGAATAGTGAATGGAAAACAATAGAAGACACAAAGGACCCCTATGTTGAAGATCTGGCAAGGTTTTCAGTGGCAAAGTTTACACACGATCAGCACATCCAAGAAATGGTGACGTTCATTCGAGTGGTGAGTAGTGAGTGTCTAGTGATGAATGAAGGAACTATGTATCGATTGGTGGTAGAGAGTAGAAAAGAGAATGTAACTCAATCACGTGAGATATATGTGGCTAAGGTATTAGATCATAAGCATCCTTTCAATTCTTGGACACTCTTATCTTTTGAATGCTCTACCCTTTTAGCTTTGCCTTCTAGCTTGGCGCTCTAGAGAATCCTCCCATTTGGTTCATTATCTCTATCCTTTTCAATATGTGTAAATAAAATAAAAGACTTGTATATCTCTAATGCTATGTTTCTTCCTTCTTCATTATCATCCCAACCTTGTGAAAGAGTTATCAACATGCTAGCGAAAGCAATTAGAATCTAAAACTCTTTAAATGTATGAAATTGAATGATTAAGTATGCTTGTATTTAGGCAAAATTGATGAACTTACATTGGTGTAGCTTGAAATTCAAGCAATATTTTGATCAAGAACACTTTGTAGACCACTGCTAGTGTTTTTTTCCCTCTATTCTCTAGCATTAGAACAGTTTGTGGGAGCGAAATCTGTGATGAACTTGAAGTTATTTTGAAGATTTTGG

Coding sequence (CDS)

ATGAGTGATATGTTAGTCTCTCCTCGAGGAGCTAATTTGTTGGTTCAAAATCACAAGAATAGTGAATGGAAAACAATAGAAGACACAAAGGACCCCTATGTTGAAGATCTGGCAAGGTTTTCAGTGGCAAAGTTTACACACGATCAGCACATCCAAGAAATGGTGACGTTCATTCGAGTGGTGAGTAGTGAGTGTCTAGTGATGAATGAAGGAACTATGTATCGATTGGTGGTAGAGAGTAGAAAAGAGAATGTAACTCAATCACGTGAGATATATGTGGCTAAGGTATTAGATCATAAGCATCCTTTCAATTCTTGGACACTCTTATCTTTTGAATGCTCTACCCTTTTAGCTTTGCCTTCTAGCTTGGCGCTCTAG

Protein sequence

MSDMLVSPRGANLLVQNHKNSEWKTIEDTKDPYVEDLARFSVAKFTHDQHIQEMVTFIRVVSSECLVMNEGTMYRLVVESRKENVTQSREIYVAKVLDHKHPFNSWTLLSFECSTLLALPSSLAL
Homology
BLAST of PI0008466 vs. ExPASy TrEMBL
Match: A0A5A7UB42 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold48614G00010 PE=4 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.1e-52
Identity = 107/125 (85.60%), Postives = 117/125 (93.60%), Query Frame = 0

Query: 1   MSDMLVSPRGANLLVQNHKNSEWKTIEDTKDPYVEDLARFSVAKFTHDQHIQEMVTFIRV 60
           MS+MLVSP+ ANLLVQNHKNSEWK IEDTKDPY+ED+ RFSVAKFT +Q I+EM TFIRV
Sbjct: 1   MSNMLVSPQEANLLVQNHKNSEWKAIEDTKDPYIEDMGRFSVAKFTRNQDIEEMATFIRV 60

Query: 61  VSSECLVMNEGTMYRLVVESRKENVTQSREIYVAKVLDHKHPFNSWTLLSFECSTLLALP 120
           VSSE LV+NEGTMYR+VVE RKENV+QSRE+YVAKVLDHKHPFNSWTLLSFECSTLLALP
Sbjct: 61  VSSEYLVVNEGTMYRIVVEIRKENVSQSRELYVAKVLDHKHPFNSWTLLSFECSTLLALP 120

Query: 121 SSLAL 126
           SSLAL
Sbjct: 121 SSLAL 125

BLAST of PI0008466 vs. ExPASy TrEMBL
Match: A0A6J1BX23 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005501 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 3.8e-05
Identity = 41/118 (34.75%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 1   MSDMLVSPRGANLLVQNHKNSEWKTIEDTKDPYVEDLARFSVAKFTHDQHIQEMVTFIRV 60
           M  + V+P   + L    K   W+ I++ KDPYV+++ RF+V +      +   + F+ V
Sbjct: 32  MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 91

Query: 61  VSSECLVMNEGTMYRLVVESR-KENV------TQSREIYVAKVLDHKHPFNSWTLLSF 112
           VS E  ++  G  YRLVVE +  E +        S  +YVA VLD K    SW +LSF
Sbjct: 92  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLD-KPSDKSWKVLSF 148

BLAST of PI0008466 vs. ExPASy TrEMBL
Match: A0A6J1BUK6 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005500 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.5e-04
Identity = 42/119 (35.29%), Postives = 61/119 (51.26%), Query Frame = 0

Query: 1   MSDMLVSPRGANLLVQNHKNSEWKTIEDTKDPYVEDLARFSV-AKFTHDQHIQEMVTFIR 60
           M  + V+P   + L+       W+ I++ KDPYV+++ RF+V  +      +   + FI 
Sbjct: 1   MDSVAVAPAQNDALLHLKMPGGWEPIKNIKDPYVQEMGRFAVMEQHPKPSELIASMEFIG 60

Query: 61  VVSSECLVMNEGTMYRLVVESR-KENV------TQSREIYVAKVLDHKHPFNSWTLLSF 112
           VVS E  ++  G  YRLVVE R  E +        S  +YVA VLD K    SW +LSF
Sbjct: 61  VVSGEIQLVAHGKEYRLVVEVRVTEKIIVHGCPVSSIRLYVAVVLD-KPSDKSWKVLSF 118

BLAST of PI0008466 vs. NCBI nr
Match: KAA0053163.1 (cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa] >TYK01666.1 cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 215.7 bits (548), Expect = 2.3e-52
Identity = 107/125 (85.60%), Postives = 117/125 (93.60%), Query Frame = 0

Query: 1   MSDMLVSPRGANLLVQNHKNSEWKTIEDTKDPYVEDLARFSVAKFTHDQHIQEMVTFIRV 60
           MS+MLVSP+ ANLLVQNHKNSEWK IEDTKDPY+ED+ RFSVAKFT +Q I+EM TFIRV
Sbjct: 1   MSNMLVSPQEANLLVQNHKNSEWKAIEDTKDPYIEDMGRFSVAKFTRNQDIEEMATFIRV 60

Query: 61  VSSECLVMNEGTMYRLVVESRKENVTQSREIYVAKVLDHKHPFNSWTLLSFECSTLLALP 120
           VSSE LV+NEGTMYR+VVE RKENV+QSRE+YVAKVLDHKHPFNSWTLLSFECSTLLALP
Sbjct: 61  VSSEYLVVNEGTMYRIVVEIRKENVSQSRELYVAKVLDHKHPFNSWTLLSFECSTLLALP 120

Query: 121 SSLAL 126
           SSLAL
Sbjct: 121 SSLAL 125

BLAST of PI0008466 vs. NCBI nr
Match: KAG6603238.1 (Cysteine proteinase inhibitor 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 110.5 bits (275), Expect = 1.0e-20
Identity = 60/115 (52.17%), Postives = 79/115 (68.70%), Query Frame = 0

Query: 15  VQNHKNSEWKTIEDTKDPYVEDLARFSVAKFTHDQHIQEMVTFIRVVSSECLVMNEGTMY 74
           +Q  K  +WK+IED KDPYV+++ RF++ KF H Q   +  TFIRVV+ E L + EG +Y
Sbjct: 10  LQACKPHDWKSIEDIKDPYVQEIGRFAMTKFIHHQ-TGKTWTFIRVVNGETLAVEEGMLY 69

Query: 75  RLVVESRKENVTQSR----EIYVAKVLDHKHPFNSWTLLSFECSTLLALPSSLAL 126
           R+V+E + + V QS     E+YVAKVLDHK P  SW LL+FE S LLALP  LA+
Sbjct: 70  RVVIEVKTDIVVQSYLGSIELYVAKVLDHKQPRKSWELLAFESSALLALPPGLAI 123

BLAST of PI0008466 vs. NCBI nr
Match: KAG6590493.1 (hypothetical protein SDJN03_15916, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-09
Identity = 44/116 (37.93%), Postives = 70/116 (60.34%), Query Frame = 0

Query: 1   MSDMLVSPRGANLLVQNHKNSEWKTIEDTKDPYVEDLARFSVAKFTHDQHIQEMVTFIRV 60
           M  ++V+PR   +L QNHK  +W+ I++  DPYV+++ RF+V +  H   +    +F+ V
Sbjct: 1   MDPVVVAPRQDGVLNQNHKQCDWELIKNKDDPYVQEMGRFAVMEHVHGL-VGAKWSFVGV 60

Query: 61  VSSECLVMNEGTMYRLVVESRKENVT----QSREIYVAKVLDHKHPFNSWTLLSFE 113
           VS E LV+ +G +YRL+++   E V      + E+Y A VLD K    SW L+SF+
Sbjct: 61  VSCESLVVPKGKLYRLLIKVDIEIVVPCHPHAIEVYEAIVLD-KECERSWELMSFK 114

BLAST of PI0008466 vs. NCBI nr
Match: KAG6590492.1 (hypothetical protein SDJN03_15915, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-07
Identity = 43/116 (37.07%), Postives = 66/116 (56.90%), Query Frame = 0

Query: 1   MSDMLVSPRGANLLVQNHKNSEWKTIEDTKDPYVEDLARFSVAKFTHDQHIQEMVTFIRV 60
           M  ++V+PR   +L QNHK  +W+ I++  DPYV ++ RF+V +  H+    E   F+ V
Sbjct: 1   MDSIVVAPRQDGVLNQNHKQCDWELIKNKDDPYVHEMGRFAVMEHAHNLLGAEW-KFVSV 60

Query: 61  VSSECLVMNEGTMYRLVVESRKENV----TQSREIYVAKVLDHKHPFNSWTLLSFE 113
           +S +  V+ +G +Y LV+E     V      S E+Y A VLD K    SW L+SF+
Sbjct: 61  LSCKTQVVPKGKLYCLVIEVDIAIVIPSYPHSIELYEAIVLD-KQCERSWELISFD 114

BLAST of PI0008466 vs. NCBI nr
Match: WP_198843152.1 (hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical protein [Aureibaculum sp. A20])

HSP 1 Score: 61.6 bits (148), Expect = 5.5e-06
Identity = 44/114 (38.60%), Postives = 65/114 (57.02%), Query Frame = 0

Query: 5   LVSPRGANLLVQNH-KNSEWKTIEDTKDPYVEDLARFSVAKFTHDQHIQEMVTFIRVVSS 64
           +++P   + L QN  K   W+ I++  DPYV+++ RFSV + TH + +   + FIRVVS 
Sbjct: 9   VIAPGQNDALHQNRCKPGGWEPIKNIHDPYVQEMGRFSVMEHTH-KLVGASMKFIRVVSG 68

Query: 65  ECLVMNEGTMYRLVVESRKENVT------QSREIYVAKVLDHKHPFNSWTLLSF 112
           E   M+EG  YRLVVE  +  +T       S + Y+A VL+ K    SW L +F
Sbjct: 69  ETRPMSEGKEYRLVVEVAEHIITCPPMLSVSVKFYLATVLE-KPLDRSWQLEAF 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7UB421.1e-5285.60Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1BX233.8e-0534.75cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1BUK61.5e-0435.29cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005... [more]
Match NameE-valueIdentityDescription
KAA0053163.12.3e-5285.60cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa] >TYK01666.1 cyst... [more]
KAG6603238.11.0e-2052.17Cysteine proteinase inhibitor 6, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6590493.11.4e-0937.93hypothetical protein SDJN03_15916, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6590492.11.7e-0737.07hypothetical protein SDJN03_15915, partial [Cucurbita argyrosperma subsp. sorori... [more]
WP_198843152.15.5e-0638.60hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.450.10coord: 19..115
e-value: 5.7E-13
score: 50.7
NoneNo IPR availablePANTHERPTHR47364CYSTEINE PROTEINASE INHIBITOR 5coord: 17..114
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 22..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0008466.1PI0008466.1mRNA