PI0008465 (gene) Melon (PI 482460) v1

Overview
NamePI0008465
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionHeparanase-like protein 1
Locationchr09: 11669843 .. 11672951 (+)
RNA-Seq ExpressionPI0008465
SyntenyPI0008465
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCATGTGGTAGGAATTTTGGAATCAACCATTTATGGAATACCAAATATTCCTTTTGATTTTGGTTTTTGCTTTCATCCCAAGAACTATTTTGGTCATAATGTTACAATAGGAAAAATTGTAGTTGATGGAACTACAAAAATATCGGAAACAGATGAGAATTTCATTTGTTTTACTTTGGACATTTGGTCTCATGATGAGTGTAGTCAACCCAACCTTTGTGCTTGGGATGGTCATGCATCGATGCTTAATTTGGTAAGAATTATTGATCTTAGCTTGGATATGAAAAGAAAGTAATTCATAGTATATTTTATTAATTAATCAATTTTTTTTCTAGGATCTGTCTCTTCCTATTCTTAACAAAGCTGTTCGATGTAATTTGGTTCTTTGAAATATATAAATAAATTAGTAATTAATCTTTATATTAAAATTTTAACTTAATGTTATGCGTGGTTTGCATGATAGTAGCTTTCAAGACATTAAAAATTAGAGTAGGAGGTACCTTACAAGACAGGTTGATTTACGATATTGGTGATGGTTTCAAGGGAAATTGTCATCCATTTCAAGCCGACAAAGATTTACTTTTTGACTTTACAGAAGGTTGTTTATACATGGAAAGATGGGATGATTTGAACAAATTTTTCAACAATACAGGGTATGCTTATCTTTCTTTTCACAACCCAACTATTTATAATCTTTTTTTTTTCGTATATCTTTTTGTTATTATATATATATATATATATACATTTTCACCGTTACTTTTCTTTTTTTTGTAAAAACAAATATACAATAGGGCAATTGTAACTTTTGGCTTAAATGCTCTACTGGGCAAGTACAACACAAAAGGAATACAATGGGAAGGCAATTGGAACTACAGGAATGCTGAGGCTCTTGTTAAATATACAGTGGACAAGAAGTATAATATAAATTCATGGGAGTTTGGTAAGTTAAACAATACCAAATCTCAATTTTCTTGTTAAGTCATTATGAATGTAATTCACGTGGCGTGGTTGGAGAATGATATCCTTGTCCACAACCTCCATCCAAATCTGTGTCTCAAAATTTACCATCTGGATTGAGATTTCTTTTTTTGGAATAATTATTCTTCATTATTTAAAAAGATTTGATTTCAATTAATGGGGAAAAAGTTTACGTTTCATCGTTACAACATGTATTCTGTTACAAAACATATCGTTTGAAATTGGGAGAGAAAATTCTCCTTGTTCTTTCTTTCCACGTGGGAGTTGTGATCTTACATGATGAAATAAATGAACTATATCTCTATAAATAACTATATACATCATATTATAATTGAGGAAGCATATGTGATTATATTCTTGGAATACGTAGGCAATGAATTGGCTGGCTAGCGTTAATGCTTCACAATATGCAAAAAATCTACTGAAACTTCGAGAAATCATAGATCGTTTGTACAAGAATTCCCAACAAAAACCTTTGATTGTTGCACCTGGTGCATTCTTTGATGACAAATGGTATGATGAACTTGTTACAAAAACTGGATCAAATGTTGTTAGTACTCTCACTCATCATATATATATATATAACATGGGTGCAGGTAAATTAGCTTATATATTAGTTAATAATCTTTTACTTAACTATGTATTGATTATCATTATAATTCAACTATTAATTCTTTTAATTTTGGTTTATGTATATATATAGGTGATGACCCCAAATTGATTTAAAAATTTGTTAATCCAACATACCTAAGTCAAGTATCAAACACATTTAGACAACTAAAGAATATAATTGAAAAGCATGCCCCTTGGGCTTCTGCTTGGGTTGGTGAAGCTGGTGGAGCCTACCATGGTGGCAGTCCTCATATTTCTGATGCATTTATCAATAGTTTTTGGTAACAGATTAAATTTTATTTTACATTTCTTCTATATTTTTTTCTCTCCACTTTATTTAAATATGAATTGAATTATGGAGGTATTTAGATCAACTTGGGATGGCCTCTTTATACAATACCAAATACCAAAGTACATTGTAGGCAAACTTTGATAGGTGGATTTTACAATATTCTTAGAGCTAAAACTTATATTCCTACCCTAGACTACTATGGGTAAGATTATTAAATTTGAAAGAGTTTAAAATCCTACCAATTTTTCTTGACATTTAAATGTTATATAGTCAAGTGGGTTGTCTTATGAGCTTGCCTGAACATCATGAATATCAAAAGAATTGAAATATTTTTCTCATCTGATATGTTTTTTTTTTTTAAATTTGTTTAGTGCACTTCTCTTCCACCGACTTATGGGCTCAAGTGCTCTCAAAGTTGATAATAATGTCTCTTCTTATCTTCGCACCTATGCTCATTGCTCGAGAGGAAAAGTAAGAAATCTCTTTTCAAATTTAAATTTAGAAAAAATTGATGATTCATTTGATAACTAATTGATTTTTGGTTACAAGTTTTTGAAAATTAAGTCTATAAACATAATTTCGCACTTTTAAATTTCATGTTTTTTTTATCTACTTTTTACCAACTTTTTCAAAATCCAAGACAAAATTTAAAAACTAAAAAAAAAAAATTATGTTTAAAAACTTAACTATACTTTTTTTAGAATTTGGTTAAGAATTGAATCTTTTTATTTAAAAACGATGCAAACCATTGTACAAAACTTAGAGAAAATAGAGTTAATCTTCAAAAACCAAAAGCCCTAGAATCATATGGTTGTTGAACGAAATCTAAATAAATACGGGTAATTGATTTGAATTGATTATATATATAATTTTATTGCAGTCCGGTGTAACCATGCTTTTCATCAACTTGAGCAATACAACAAAGTTCACAATAAAGATTGAAAACCATATGAACTTGAGTTTGCACAAAAGCAAACCCAAGAATAGTTCACCATCAAAGAATGTGGGAACACAAAGAGAGGAATATCATTTGACACCAGAAAATGGTATTATTAGAAGTTCTACGGTGCTTTTGAATGGAAGAGAATTGGAGCTTACAAAAGAAGGAGAATTGCCAGATCTTGTACCTGTCTATAGAGATAGTAACTCTTCTATAAGTATTGCTAATTGGTCCATTACTTTCATTGTCATCCCTGACTTTGTAGCCGTTGGATGCAATTAA

mRNA sequence

ATGTTGCATGTGGTAGGAATTTTGGAATCAACCATTTATGGAATACCAAATATTCCTTTTGATTTTGGTTTTTGCTTTCATCCCAAGAACTATTTTGGTCATAATGTTACAATAGGAAAAATTGTAGTTGATGGAACTACAAAAATATCGGAAACAGATGAGAATTTCATTTGTTTTACTTTGGACATTTGGTCTCATGATGAGTGTAGTCAACCCAACCTTTGTGCTTGGGATGGTCATGCATCGATGCTTAATTTGGATCTGTCTCTTCCTATTCTTAACAAAGCTGTTCGATCTTTCAAGACATTAAAAATTAGAGTAGGAGGTACCTTACAAGACAGGTTGATTTACGATATTGGTGATGGTTTCAAGGGAAATTGTCATCCATTTCAAGCCGACAAAGATTTACTTTTTGACTTTACAGAAGGTTGTTTATACATGGAAAGATGGGATGATTTGAACAAATTTTTCAACAATACAGGGGCAATTGTAACTTTTGGCTTAAATGCTCTACTGGGCAAGTACAACACAAAAGGAATACAATGGGAAGGCAATTGGAACTACAGGAATGCTGAGGCTCTTGTTAAATATACAGTGGACAAGAAGTATAATATAAATTCATGGGAGTTTGGTAACGTTAATGCTTCACAATATGCAAAAAATCTACTGAAACTTCGAGAAATCATAGATCGTTTGTACAAGAATTCCCAACAAAAACCTTTGATTGTTGCACCTGGTGCATTCTTTGATGACAAATGGTATGATGAACTTGTTACAAAAACTGGATCAAATGTTGTTAGTACTCTCACTCATCATATATATATATATAACATGGGTGCAGTATCAAACACATTTAGACAACTAAAGAATATAATTGAAAAGCATGCCCCTTGGGCTTCTGCTTGGGTTGGTGAAGCTGGTGGAGCCTACCATGGTGGCAGTCCTCATATTTCTGATGCATTTATCAATAGTTTTTGGCAAACTTTGATAGGTGGATTTTACAATATTCTTAGAGCTAAAACTTATATTCCTACCCTAGACTACTATGGTGCACTTCTCTTCCACCGACTTATGGGCTCAAGTGCTCTCAAAGTTGATAATAATGTCTCTTCTTATCTTCGCACCTATGCTCATTGCTCGAGAGGAAAATCCGGTGTAACCATGCTTTTCATCAACTTGAGCAATACAACAAAGTTCACAATAAAGATTGAAAACCATATGAACTTGAGTTTGCACAAAAGCAAACCCAAGAATAGTTCACCATCAAAGAATGTGGGAACACAAAGAGAGGAATATCATTTGACACCAGAAAATGGTATTATTAGAAGTTCTACGGTGCTTTTGAATGGAAGAGAATTGGAGCTTACAAAAGAAGGAGAATTGCCAGATCTTGTACCTGTCTATAGAGATAGTAACTCTTCTATAAGTATTGCTAATTGGTCCATTACTTTCATTGTCATCCCTGACTTTGTAGCCGTTGGATGCAATTAA

Coding sequence (CDS)

ATGTTGCATGTGGTAGGAATTTTGGAATCAACCATTTATGGAATACCAAATATTCCTTTTGATTTTGGTTTTTGCTTTCATCCCAAGAACTATTTTGGTCATAATGTTACAATAGGAAAAATTGTAGTTGATGGAACTACAAAAATATCGGAAACAGATGAGAATTTCATTTGTTTTACTTTGGACATTTGGTCTCATGATGAGTGTAGTCAACCCAACCTTTGTGCTTGGGATGGTCATGCATCGATGCTTAATTTGGATCTGTCTCTTCCTATTCTTAACAAAGCTGTTCGATCTTTCAAGACATTAAAAATTAGAGTAGGAGGTACCTTACAAGACAGGTTGATTTACGATATTGGTGATGGTTTCAAGGGAAATTGTCATCCATTTCAAGCCGACAAAGATTTACTTTTTGACTTTACAGAAGGTTGTTTATACATGGAAAGATGGGATGATTTGAACAAATTTTTCAACAATACAGGGGCAATTGTAACTTTTGGCTTAAATGCTCTACTGGGCAAGTACAACACAAAAGGAATACAATGGGAAGGCAATTGGAACTACAGGAATGCTGAGGCTCTTGTTAAATATACAGTGGACAAGAAGTATAATATAAATTCATGGGAGTTTGGTAACGTTAATGCTTCACAATATGCAAAAAATCTACTGAAACTTCGAGAAATCATAGATCGTTTGTACAAGAATTCCCAACAAAAACCTTTGATTGTTGCACCTGGTGCATTCTTTGATGACAAATGGTATGATGAACTTGTTACAAAAACTGGATCAAATGTTGTTAGTACTCTCACTCATCATATATATATATATAACATGGGTGCAGTATCAAACACATTTAGACAACTAAAGAATATAATTGAAAAGCATGCCCCTTGGGCTTCTGCTTGGGTTGGTGAAGCTGGTGGAGCCTACCATGGTGGCAGTCCTCATATTTCTGATGCATTTATCAATAGTTTTTGGCAAACTTTGATAGGTGGATTTTACAATATTCTTAGAGCTAAAACTTATATTCCTACCCTAGACTACTATGGTGCACTTCTCTTCCACCGACTTATGGGCTCAAGTGCTCTCAAAGTTGATAATAATGTCTCTTCTTATCTTCGCACCTATGCTCATTGCTCGAGAGGAAAATCCGGTGTAACCATGCTTTTCATCAACTTGAGCAATACAACAAAGTTCACAATAAAGATTGAAAACCATATGAACTTGAGTTTGCACAAAAGCAAACCCAAGAATAGTTCACCATCAAAGAATGTGGGAACACAAAGAGAGGAATATCATTTGACACCAGAAAATGGTATTATTAGAAGTTCTACGGTGCTTTTGAATGGAAGAGAATTGGAGCTTACAAAAGAAGGAGAATTGCCAGATCTTGTACCTGTCTATAGAGATAGTAACTCTTCTATAAGTATTGCTAATTGGTCCATTACTTTCATTGTCATCCCTGACTTTGTAGCCGTTGGATGCAATTAA

Protein sequence

MLHVVGILESTIYGIPNIPFDFGFCFHPKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINSWEFGNVNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDELVTKTGSNVVSTLTHHIYIYNMGAVSNTFRQLKNIIEKHAPWASAWVGEAGGAYHGGSPHISDAFINSFWQTLIGGFYNILRAKTYIPTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKIENHMNLSLHKSKPKNSSPSKNVGTQREEYHLTPENGIIRSSTVLLNGRELELTKEGELPDLVPVYRDSNSSISIANWSITFIVIPDFVAVGCN
Homology
BLAST of PI0008465 vs. ExPASy Swiss-Prot
Match: Q9FF10 (Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 3.9e-130
Identity = 231/528 (43.75%), Postives = 333/528 (63.07%), Query Frame = 0

Query: 28  PKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLD 87
           P+      +    IV+ G  ++ ETDENF+C TLD W HD+C+  + C W G++S++N+D
Sbjct: 19  PEKTMAQEMKRASIVIQGARRVCETDENFVCATLDWWPHDKCNY-DQCPW-GYSSVINMD 78

Query: 88  LSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYM 147
           L+ P+L KA+++FK L+IR+GG+LQD++IYD+G+  K  C PFQ     LF F++GCL+M
Sbjct: 79  LTRPLLTKAIKAFKPLRIRIGGSLQDQVIYDVGN-LKTPCRPFQKMNSGLFGFSKGCLHM 138

Query: 148 ERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINS 207
           +RWD+LN F   TGA+VTFGLNAL G++  +G  W G W++ N +  + YTV K Y I+S
Sbjct: 139 KRWDELNSFLTATGAVVTFGLNALRGRHKLRGKAWGGAWDHINTQDFLNYTVSKGYVIDS 198

Query: 208 WEFGN----------VNASQYAKNLLKLREIIDRLYKNS-QQKPLIVAPGAFFDDKWYDE 267
           WEFGN          V+A  Y K+L+ L+++I+++YKNS   KP++VAPG F++ +WY +
Sbjct: 199 WEFGNELSGSGVGASVSAELYGKDLIVLKDVINKVYKNSWLHKPILVAPGGFYEQQWYTK 258

Query: 268 LVTKTGSNVVSTLTHHIYIYNMG----------------AVSNTFRQLKNIIEKHAPWAS 327
           L+  +G +VV  +THHIY    G                 VS TF+ +   I++H PWAS
Sbjct: 259 LLEISGPSVVDVVTHHIYNLGSGNDPALVKKIMDPSYLSQVSKTFKDVNQTIQEHGPWAS 318

Query: 328 AWVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTY 387
            WVGE+GGAY+ G  H+SD FI+SFW                  QTL+GGFY +L   T+
Sbjct: 319 PWVGESGGAYNSGGRHVSDTFIDSFWYLDQLGMSARHNTKVYCRQTLVGGFYGLLEKGTF 378

Query: 388 IPTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIK 447
           +P  DYY ALL+HRLMG   L V  +    LR YAHCS+G++GVT+L INLSN + FT+ 
Sbjct: 379 VPNPDYYSALLWHRLMGKGVLAVQTDGPPQLRVYAHCSKGRAGVTLLLINLSNQSDFTVS 438

Query: 448 IENHMNLSLHKSKPKNSS-------PSKNVGTQ-------REEYHLTPENGIIRSSTVLL 497
           + N +N+ L+    K  S       P   +G++       REEYHLTPENG++RS T++L
Sbjct: 439 VSNGINVVLNAESRKKKSLLDTLKRPFSWIGSKASDGYLNREEYHLTPENGVLRSKTMVL 498

BLAST of PI0008465 vs. ExPASy Swiss-Prot
Match: Q8L608 (Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 3.4e-126
Identity = 230/527 (43.64%), Postives = 322/527 (61.10%), Query Frame = 0

Query: 28  PKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLD 87
           P   FG N+    +V+DG+ +I+ETDENFIC TLD W  ++C+  + C W G+AS++NL+
Sbjct: 16  PPVTFGSNMERTTLVIDGSRRIAETDENFICATLDWWPPEKCNY-DQCPW-GYASLINLN 75

Query: 88  LSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYM 147
           L+ P+L KA+++F+TL+IR+GG+LQD++IYD+GD  K  C  F+   D LF F+EGCLYM
Sbjct: 76  LASPLLAKAIQAFRTLRIRIGGSLQDQVIYDVGD-LKTPCTQFKKTDDGLFGFSEGCLYM 135

Query: 148 ERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINS 207
           +RWD++N FFN TGAIVTFGLNAL G+    G  W G+W++ N +  + YTV K Y I+S
Sbjct: 136 KRWDEVNHFFNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTVSKGYAIDS 195

Query: 208 WEFGN----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDEL 267
           WEFGN          V+   Y K+L+ L+ +I  +YKNS+ KPL+VAPG FF+++WY EL
Sbjct: 196 WEFGNELSGSGIWASVSVELYGKDLIVLKNVIKNVYKNSRTKPLVVAPGGFFEEQWYSEL 255

Query: 268 VTKTGSNVVSTLTHHIYIYNMG----------------AVSNTFRQLKNIIEKHAPWASA 327
           +  +G  V+  LTHHIY    G                 +S  F  +   I++H PWA+A
Sbjct: 256 LRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGISELFANVNQTIQEHGPWAAA 315

Query: 328 WVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTYI 387
           WVGEAGGA++ G   +S+ FINSFW                  Q L+GGFY +L  +T++
Sbjct: 316 WVGEAGGAFNSGGRQVSETFINSFWYLDQLGISSKHNTKVYCRQALVGGFYGLLEKETFV 375

Query: 388 PTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKI 447
           P  DYY ALL+HRLMG   L V    S YLR Y HCS+ ++G+T+L INLS  T FT+ +
Sbjct: 376 PNPDYYSALLWHRLMGKGILGVQTTASEYLRAYVHCSKRRAGITILLINLSKHTTFTVAV 435

Query: 448 ENHMNLSLHKSKPKNSSPSKNVGTQ--------------REEYHLTPENGIIRSSTVLLN 497
            N + + L     K  S  + + ++              REEYHL+P++G +RS  +LLN
Sbjct: 436 SNGVKVVLQAESMKRKSFLETIKSKVSWVGNKASDGYLNREEYHLSPKDGDLRSKIMLLN 495

BLAST of PI0008465 vs. ExPASy Swiss-Prot
Match: Q9FZP1 (Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2)

HSP 1 Score: 366.3 bits (939), Expect = 5.4e-100
Identity = 201/509 (39.49%), Postives = 285/509 (55.99%), Query Frame = 0

Query: 39  GKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLDLSLPILNKAVR 98
           G + V G   +   DE+FIC TLD W  ++C   + C+WD HAS+LNLDL+  IL  A++
Sbjct: 31  GTVFVYGRAAVGTIDEDFICATLDWWPPEKCDYGS-CSWD-HASILNLDLNNVILQNAIK 90

Query: 99  SFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYMERWDDLNKFFN 158
           +F  LKIR+GGTLQD +IY+  D  K  C PF  +  +LF +T+GCL M RWD+LN FF 
Sbjct: 91  AFAPLKIRIGGTLQDIVIYETPDS-KQPCLPFTKNSSILFGYTQGCLPMRRWDELNAFFR 150

Query: 159 NTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINSWEFGN------ 218
            TG  V FGLNAL G+      +  G WNY NAE+ +++T +  Y I+ WE GN      
Sbjct: 151 KTGTKVIFGLNALSGRSIKSNGEAIGAWNYTNAESFIRFTAENNYTIDGWELGNELCGSG 210

Query: 219 ----VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDELVTKTGSNVVST 278
               V A+QYA + + LR I++R+YKN    PL++ PG FF+  W+ E + K   N ++ 
Sbjct: 211 VGARVGANQYAIDTINLRNIVNRVYKNVSPMPLVIGPGGFFEVDWFTEYLNK-AENSLNA 270

Query: 279 LTHHIYIYNMGA----------------VSNTFRQLKNIIEKHAPWASAWVGEAGGAYHG 338
            T HIY    G                  + +FR LKNII+  +  A AWVGE+GGAY+ 
Sbjct: 271 TTRHIYDLGPGVDEHLIEKILNPSYLDQEAKSFRSLKNIIKNSSTKAVAWVGESGGAYNS 330

Query: 339 GSPHISDAFINSFW------------------QTLIGGFYNILRAKTYIPTLDYYGALLF 398
           G   +S+AF+ SFW                  Q+LIGG Y +L    + P  DYY AL++
Sbjct: 331 GRNLVSNAFVYSFWYLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTNFTPNPDYYSALIW 390

Query: 399 HRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKIENHMNLSLHKS 458
            +LMG  AL    + +  +R+Y HC+R   G+T+L +NL NTT    K+E + + SL  +
Sbjct: 391 RQLMGRKALFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNSFSLRHT 450

Query: 459 KPKNSSP--------SKNVGTQREEYHLTPENGIIRSSTVLLNGRELELTKEGELPDLVP 496
           K   S            N   QREEYHLT ++G + S T+LLNG  L++   G+LP + P
Sbjct: 451 KHMKSYKRASSQLFGGPNGVIQREEYHLTAKDGNLHSQTMLLNGNALQVNSMGDLPPIEP 510

BLAST of PI0008465 vs. ExPASy Swiss-Prot
Match: Q9LRC8 (Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.8e-85
Identity = 188/514 (36.58%), Postives = 271/514 (52.72%), Query Frame = 0

Query: 33  GHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLDLSLPI 92
           G   TI KI       +++TDEN++C TLD+W   +C+  N C W G +S LNLDL+  I
Sbjct: 25  GEETTIVKI---EENPVAQTDENYVCATLDLWPPTKCNYGN-CPW-GKSSFLNLDLNNNI 84

Query: 93  LNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYMERWDD 152
           +  AV+ F  LK+R GGTLQDRL+Y        +   F  + +L+ DF+  CL ++RWD+
Sbjct: 85  IRNAVKEFAPLKLRFGGTLQDRLVYQTSRDEPCD-STFYNNTNLILDFSHACLSLDRWDE 144

Query: 153 LNKFFNNTGAIVTFGLNALLGK-YNTKGIQWE-----------GNWNYRNAEALVKYTVD 212
           +N+F   TG+   FGLNAL GK    KGI  +           G W+Y N++ L++Y++ 
Sbjct: 145 INQFILETGSEAVFGLNALRGKTVEIKGIIKDGQYLGETTTAVGEWDYSNSKFLIEYSLK 204

Query: 213 KKY-NINSWEFGN----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFF 272
           K Y +I  W  GN          V+   YA +  KL E++  +Y++    PLI+APGA F
Sbjct: 205 KGYKHIRGWTLGNELGGHTLFIGVSPEDYANDAKKLHELVKEIYQDQGTMPLIIAPGAIF 264

Query: 273 DDKWYDELVTKTGSNVVSTLTHHIYIYNMG-----------------AVSNTFRQLKNII 332
           D +WY E + +T    +   THH+Y    G                 A  + +  L+ I+
Sbjct: 265 DLEWYTEFIDRTPE--LHVATHHMYNLGSGGDDALKDVLLTASFFDEATKSMYEGLQKIV 324

Query: 333 EKHAPWASAWVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFY 392
            +    A AW+GEAGGA++ G   IS+ FIN FW                  QTL GG Y
Sbjct: 325 NRPGTKAVAWIGEAGGAFNSGQDGISNTFINGFWYLNMLGYSALLDTKTFCRQTLTGGNY 384

Query: 393 NILRAKTYIPTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLS 452
            +L+  TYIP  DYY ALL+HRLMGS  LK +   +  +  YAHC++  +G+TML +N  
Sbjct: 385 GLLQTGTYIPNPDYYSALLWHRLMGSKVLKTEIVGTKNVYIYAHCAKKSNGITMLVLNHD 444

Query: 453 NTTKFTIKIENHMNLSLHKSKPKNSSPSKNVGTQREEYHLTPENGIIRSSTVLLNGRELE 489
                    E+ + +SL  SK          G++REEYHLTP N  ++S  V LNG  L 
Sbjct: 445 G--------ESSVKISLDPSK---------YGSKREEYHLTPVNNNLQSRLVKLNGELLH 504

BLAST of PI0008465 vs. ExPASy Swiss-Prot
Match: X4Y2L4 (Hyaluronoglucuronidase OS=Hirudo nipponia OX=42736 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 6.3e-24
Identity = 118/462 (25.54%), Postives = 196/462 (42.42%), Query Frame = 0

Query: 86  LDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKD---------L 145
           +D++ P L K +        RVGGT  + L +D+ +  K   +    DK           
Sbjct: 40  VDITSPKLFKLLEGLSPGYFRVGGTFANWLFFDLDENNKWKDYWAFKDKTPETATITRRW 99

Query: 146 LFDFTEGCLYMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVK 205
           LF   +  L  E +DDL K    +   + F LNA +      G +    W+   AE L K
Sbjct: 100 LFR-KQNNLKKETFDDLVKLTKGSKMRLLFDLNAEVRTGYEIGKKMTSTWDSSEAEKLFK 159

Query: 206 YTVDKKYNIN-SWEFGNVNASQYAKNLL--KLREIIDRLYKNSQQKPL-----IVAPGAF 265
           Y V K Y  N  WE GN      A NL   ++ E    L+K  ++ P      +V P   
Sbjct: 160 YCVSKGYGDNIDWELGNEPDHTSAHNLTEKQVGEDFKALHKVLEKYPTLNKGSLVGPDVG 219

Query: 266 FDDKWYDELVTKTGSNVVSTLTHHIYIY--NMGAVS-----NTFRQLKNIIE------KH 325
           +    Y + +     + V+  T H Y +  N   VS       F++L+ + +      K+
Sbjct: 220 WMGVSYVKGLADGAGDHVTAFTLHQYYFDGNTSDVSTYLDATYFKKLQQLFDKVKDVLKN 279

Query: 326 APWAS--AWVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYN 385
           +P      W+GE    Y+ G+  +SD +++ F                   QT+  G+Y 
Sbjct: 280 SPHKDKPLWLGETSSGYNSGTKDVSDRYVSGFLTLDKLGLSAANNVKVVIRQTIYNGYYG 339

Query: 386 ILRAKTYIPTLDYYGALLFHRLMGSSALKVD-NNVSSYLRTYAHCSRGKSGVTMLFINLS 445
           +L   T  P  DY+   + + L+G++  KVD ++ ++  R YA C++  S  T       
Sbjct: 340 LLDKNTLEPNPDYWLMHVHNSLVGNTVFKVDVSDPTNKARVYAQCTKTNSKHTQSRYYKG 399

Query: 446 NTTKFTIKI-ENHMNLSLHKSKPKNSSPSKNVGTQREEYHLTPENGIIRSSTVLLNGREL 496
           + T F + + +  + L + +            G +   Y LTPE G + S  VLLNG+EL
Sbjct: 400 SLTIFALNVGDEDVTLKIDQYS----------GKKIYSYILTPEGGQLTSQKVLLNGKEL 459

BLAST of PI0008465 vs. ExPASy TrEMBL
Match: A0A0A0KTJ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G390000 PE=3 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.9e-241
Identity = 425/544 (78.12%), Postives = 454/544 (83.46%), Query Frame = 0

Query: 1   MLHVVGILESTI-YGIPNIPFDFGFCFHPKNYFGHNVTIGKIVVDGTTKISETDENFICF 60
           ML + GILE T+ Y I        F F P+   G NVT GKIVVDGTTKI+ETDENFICF
Sbjct: 1   MLGIPGILELTMEYQI--FLLILVFAFIPRTILGLNVTTGKIVVDGTTKIAETDENFICF 60

Query: 61  TLDIWSHDECSQPNLCAWDGHASMLNLDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDI 120
           TLDIW HDECSQPNLC WDGHASMLN+DLSLPILNKAV++FKTL+IRVGGTLQDRLIY+I
Sbjct: 61  TLDIWPHDECSQPNLCVWDGHASMLNMDLSLPILNKAVQAFKTLRIRVGGTLQDRLIYNI 120

Query: 121 GDGFKGNCHPFQADKDLLFDFTEGCLYMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKG 180
           GDGFKGNC+PF+A K LLFDFTEGCLYMERWDDLN FFNNTGAIVTFGLNALLGKYNTKG
Sbjct: 121 GDGFKGNCNPFEAHKGLLFDFTEGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKYNTKG 180

Query: 181 IQWEGNWNYRNAEALVKYTVDKKYNINSWEFGN-----------VNASQYAKNLLKLREI 240
           IQWEGNWNY NAEAL+KYTV+KKYNINSWEFGN           V+ASQYAK+LLKLR+I
Sbjct: 181 IQWEGNWNYSNAEALIKYTVEKKYNINSWEFGNELAGPNSIGASVSASQYAKDLLKLRQI 240

Query: 241 IDRLYKNSQQKPLIVAPGAFFDDKWYDELVTKTGSNVVSTLTHHIYIYNMGA-------- 300
           IDRLYKNSQQKPLIVAPGAFFDDKWYDELVTKTGSNVVS LTHH  IYNMGA        
Sbjct: 241 IDRLYKNSQQKPLIVAPGAFFDDKWYDELVTKTGSNVVSALTHH--IYNMGAGDDPKLIY 300

Query: 301 ----------VSNTFRQLKNIIEKHAPWASAWVGEAGGAYHGGSPHISDAFINSFW---- 360
                     VSNTFRQLKNIIEKHAPWASAWVGEAGGAYHGG  HISD FINSFW    
Sbjct: 301 RFVNPTYLSQVSNTFRQLKNIIEKHAPWASAWVGEAGGAYHGGGLHISDTFINSFWYLDQ 360

Query: 361 --------------QTLIGGFYNILRAKTYIPTLDYYGALLFHRLMGSSALKVDNNVSSY 420
                         QTL+GG+Y +LR KT+IPT DYYGALLFHRLMGSS LKVDNNVSSY
Sbjct: 361 LGMAASYNTKVYCRQTLVGGYYGVLRTKTFIPTPDYYGALLFHRLMGSSVLKVDNNVSSY 420

Query: 421 LRTYAHCSRGKSGVTMLFINLSNTTKFTIKIENHMNLSLHKSKPKNSSPSKNVGTQREEY 480
           LRTYAHCSRG+SGVTMLFINLSNTT+FTI IENHMNLSLHKSKPK+SS SKNVGTQREEY
Sbjct: 421 LRTYAHCSRGRSGVTMLFINLSNTTEFTINIENHMNLSLHKSKPKHSS-SKNVGTQREEY 480

Query: 481 HLTPENGIIRSSTVLLNGRELELTKEGELPDLVPVYRDSNSSISIANWSITFIVIPDFVA 497
           HLTP+NG++RSSTVLLNG+ LELT EGE+PDL PVYRDSNSSISI NWSI FIVIPDFVA
Sbjct: 481 HLTPQNGLLRSSTVLLNGKALELTNEGEVPDLTPVYRDSNSSISIPNWSIAFIVIPDFVA 539

BLAST of PI0008465 vs. ExPASy TrEMBL
Match: A0A5A7TNQ5 (Heparanase-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00530 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 6.9e-223
Identity = 382/503 (75.94%), Postives = 428/503 (85.09%), Query Frame = 0

Query: 26  FHPKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLN 85
           F P+   GHNVT GKIVVDGTTKI+ETDENFICFTLDIW HDECSQPNLC WD HAS+LN
Sbjct: 14  FIPRTILGHNVTTGKIVVDGTTKIAETDENFICFTLDIWPHDECSQPNLCVWDSHASVLN 73

Query: 86  LDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCL 145
           LDLSLPILNKAV++FKTL+IRVGGTLQDRLIY+IG+GF+GNCHPF+AD  LLF+FTEGCL
Sbjct: 74  LDLSLPILNKAVQAFKTLRIRVGGTLQDRLIYNIGEGFEGNCHPFEADDSLLFEFTEGCL 133

Query: 146 YMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNI 205
           YMERWDDLNKFFNNTGA+VTFGLNALLGKY+TKG+QWEGNWN+ NAEAL+KYTVD  Y I
Sbjct: 134 YMERWDDLNKFFNNTGALVTFGLNALLGKYHTKGMQWEGNWNHTNAEALIKYTVDNNYQI 193

Query: 206 NSWEFGN-----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWY 265
           NSWEFGN           V+A+QYAK+LLKLRE++DRLY+NSQQKP+IVAPGAFFDDKWY
Sbjct: 194 NSWEFGNELGGANSIGASVSAAQYAKDLLKLREMVDRLYENSQQKPMIVAPGAFFDDKWY 253

Query: 266 DELVTKTGSNVVSTLTHHIYIYNMGAVSNTFRQLKNIIEKHAPWASAWVGEAGGAYHGGS 325
            ELVTKTG NVV+ LTHH  IYNM  VS TFRQLKNI+EKHAPW+SAWVGEAGGAYHGG+
Sbjct: 254 HELVTKTGPNVVTALTHH--IYNM--VSKTFRQLKNIVEKHAPWSSAWVGEAGGAYHGGA 313

Query: 326 PHISDAFINSFW------------------QTLIGGFYNILRAKTYIPTLDYYGALLFHR 385
             ISD FINSFW                  QTL+GGFY++L+AKT++PT DYYGALLFHR
Sbjct: 314 YRISDTFINSFWYLDQLGMASLYNTKVYCRQTLVGGFYSVLKAKTFVPTPDYYGALLFHR 373

Query: 386 LMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKIENHMNLSLHK-SK 445
           LMG   LKV N VS YLRTYAHCSR KSGVTMLFINLSNTT+FTI +E+ MNLSLHK  K
Sbjct: 374 LMGPGVLKVYNEVSPYLRTYAHCSREKSGVTMLFINLSNTTEFTINVEDPMNLSLHKRGK 433

Query: 446 PK-NSSPSKNVG-TQREEYHLTPENGIIRSSTVLLNGRELELTKEGELPDLVPVYRDSNS 497
           P  +SS + NVG T+REEYHLTP+NG++RSSTVLLNG+ LELTKEGELPDL P+YRDSNS
Sbjct: 434 PNYDSSSNDNVGTTEREEYHLTPQNGLLRSSTVLLNGKALELTKEGELPDLTPIYRDSNS 493

BLAST of PI0008465 vs. ExPASy TrEMBL
Match: A0A5A7TSB3 (Heparanase-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00460 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.9e-221
Identity = 380/511 (74.36%), Postives = 429/511 (83.95%), Query Frame = 0

Query: 26  FHPKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLN 85
           F P+   G+NVTIGKIVVDGTTKI+ETDENFICFTLDIW HDECSQPNLC WD HAS+LN
Sbjct: 14  FIPRTILGYNVTIGKIVVDGTTKIAETDENFICFTLDIWPHDECSQPNLCVWDSHASVLN 73

Query: 86  LDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCL 145
           LDLSLPILNKAV++FKTL+IRVGGTLQDRLIY+IG+GF+GNCHPF+AD  LLF+FTEGCL
Sbjct: 74  LDLSLPILNKAVQAFKTLRIRVGGTLQDRLIYNIGEGFEGNCHPFEADDSLLFEFTEGCL 133

Query: 146 YMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNI 205
           YMERWDDLNKFFNNTGA+VTFGLNALLGKY+TKG+QWEGNWN+ NAEAL+KYTVD  Y I
Sbjct: 134 YMERWDDLNKFFNNTGALVTFGLNALLGKYHTKGMQWEGNWNHTNAEALIKYTVDNNYQI 193

Query: 206 NSWEFGN-----VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDELVTK 265
           NSWEF N     V+A+QYAK+LLKLRE++DRLY+NSQQKP+IVAPGAFFDDKWY ELVTK
Sbjct: 194 NSWEFANSIGASVSAAQYAKDLLKLREMVDRLYENSQQKPMIVAPGAFFDDKWYHELVTK 253

Query: 266 TGSNVVSTLTHHIY--------IYN------MGAVSNTFRQLKNIIEKHAPWASAWVGEA 325
           TG NVV+ LTHHIY        IY       +  VS TFRQLKNI+EKHAPW+SAWVGEA
Sbjct: 254 TGPNVVTALTHHIYNMGDDPKLIYRFVNPTYLSEVSKTFRQLKNIVEKHAPWSSAWVGEA 313

Query: 326 GGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTYIPTLDY 385
           GGAYHGG+  ISD FINSFW                  QTL+GGFY++L+AKT++PT DY
Sbjct: 314 GGAYHGGAYRISDTFINSFWYLDQLGMASLYNTKVYCRQTLVGGFYSVLKAKTFVPTPDY 373

Query: 386 YGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKIENHMN 445
           YGALLFHRLMG   LKV N VS YLRTYAHCSR KSGVTMLFINLSNTT+FTI +E+ +N
Sbjct: 374 YGALLFHRLMGPGVLKVYNEVSPYLRTYAHCSREKSGVTMLFINLSNTTEFTINVEDPVN 433

Query: 446 LSLHK-SKPK-NSSPSKNVGT-QREEYHLTPENGIIRSSTVLLNGRELELTKEGELPDLV 497
           LSLHK  KP  +SS + NVGT +REEYHLTP+NG++RSSTVLLNG+ LELTKEGELPDL 
Sbjct: 434 LSLHKRGKPNYDSSSNDNVGTIEREEYHLTPQNGLLRSSTVLLNGKALELTKEGELPDLT 493

BLAST of PI0008465 vs. ExPASy TrEMBL
Match: A0A0A0KUF1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G043860 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.7e-218
Identity = 373/520 (71.73%), Postives = 425/520 (81.73%), Query Frame = 0

Query: 26  FHPKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLN 85
           F P+   G NVT+GKIVV+G TKI+ETDENFICFTLDIW HDECSQPNLC WD HAS+LN
Sbjct: 14  FIPRTILGQNVTMGKIVVEGITKIAETDENFICFTLDIWPHDECSQPNLCVWDSHASVLN 73

Query: 86  LDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCL 145
           +DLSLPI+NKAV++FKTL+IRVGGTLQDRLIY+IG+GFKGNCHPF+AD  LLFDFTEGCL
Sbjct: 74  VDLSLPIINKAVQAFKTLRIRVGGTLQDRLIYNIGEGFKGNCHPFEADDSLLFDFTEGCL 133

Query: 146 YMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNI 205
           YMERWDDLN FFNNTGAIVTFGLNALLGKY+T+G+QWEGNWNY NAEAL+KYTVDK Y I
Sbjct: 134 YMERWDDLNNFFNNTGAIVTFGLNALLGKYHTQGMQWEGNWNYTNAEALIKYTVDKNYQI 193

Query: 206 NSWEFGN-----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWY 265
           NSWEFGN           ++ASQYAK+LLKLREI+DRLYKNSQQKPLIVAPGAFFDDKWY
Sbjct: 194 NSWEFGNELAGRNSIGASISASQYAKDLLKLREIVDRLYKNSQQKPLIVAPGAFFDDKWY 253

Query: 266 DELVTKTGSNVVSTLTHHIYIYNMGA------------------VSNTFRQLKNIIEKHA 325
            ELVTKTG  VVS LTHH  IYNMGA                  VSNTF+QLKNI++KHA
Sbjct: 254 HELVTKTGPKVVSVLTHH--IYNMGAGDDPKLIYRFVNPTYLSQVSNTFKQLKNIVQKHA 313

Query: 326 PWASAWVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILR 385
           PW+SAWVGEAGGAY GG+  ISD+FINSFW                  QTLIGGFY++L+
Sbjct: 314 PWSSAWVGEAGGAYQGGAYRISDSFINSFWYLDQLGMAAFYNTKVYCRQTLIGGFYSVLK 373

Query: 386 AKTYIPTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTK 445
           AKT +PT DYYGALLFHRLMG   LKV N VS+YLRTYAHCSR +SG++MLFINLSNTT+
Sbjct: 374 AKTLVPTPDYYGALLFHRLMGPGVLKVHNKVSTYLRTYAHCSRERSGISMLFINLSNTTE 433

Query: 446 FTIKIENHMNLSLHK-SKPKNSSPS-KNVGTQREEYHLTPENGIIRSSTVLLNGRELELT 497
           F I +++HM LSLHK  KPK+ S S  N+GT REEYHLTP+NG++RSS VLLNG+ L+LT
Sbjct: 434 FAINVKDHMTLSLHKRRKPKHGSSSINNLGTPREEYHLTPQNGLLRSSNVLLNGKALQLT 493

BLAST of PI0008465 vs. ExPASy TrEMBL
Match: A0A6J1I2E3 (heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111470271 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 2.1e-195
Identity = 340/514 (66.15%), Postives = 392/514 (76.26%), Query Frame = 0

Query: 33  GHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLDLSLPI 92
           GH+ T+G IV+DGT  I+ETDENF+C TLDIW HDEC    LC WDGHASMLNLDL+LPI
Sbjct: 26  GHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHDECRWSKLCVWDGHASMLNLDLTLPI 85

Query: 93  LNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYMERWDD 152
           LNKAV++FK+++IRVGGTLQD+LIY++G GFKG CHPFQA    LFDF+ GCLYMERWDD
Sbjct: 86  LNKAVQAFKSVRIRVGGTLQDKLIYNVGTGFKGTCHPFQATVGSLFDFSAGCLYMERWDD 145

Query: 153 LNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINSWEFGN 212
           LN FFNNTGAIVTFGLNALLGK+NTKGIQWEG WNY NAEAL++YTV+K Y INSWEFGN
Sbjct: 146 LNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQYTVEKNYRINSWEFGN 205

Query: 213 -----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDELVTKT 272
                      + A+QYA++LLKLREIIDRLY NSQQKPLIVAPGAFFD+ WYDE V KT
Sbjct: 206 ELAGRLSIGASITAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKT 265

Query: 273 GSNVVSTLTHHIYIYNMGA------------------VSNTFRQLKNIIEKHAPWASAWV 332
           G  VV  LTHH  IYNMGA                  VSNTF QL+N+I+K+APWA+AWV
Sbjct: 266 GPKVVDVLTHH--IYNMGAGNDPKLIYRFVNSTYLSQVSNTFEQLENVIQKNAPWAAAWV 325

Query: 333 GEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTYIPT 392
           GEAGGAY GGSPH+SD FINSFW                  QTLIGGFY +L++ T +PT
Sbjct: 326 GEAGGAYQGGSPHLSDTFINSFWYLDQLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPT 385

Query: 393 LDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKIEN 452
            DYYGALLFHRLMG   LK++N VSS LR+YAHCSRG+SGVT+L INLSNTT F I ++N
Sbjct: 386 PDYYGALLFHRLMGPGVLKINNKVSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITVQN 445

Query: 453 HMNLSLHKSKPK-NSSPSK---NVGTQREEYHLTPENGIIRSSTVLLNGRELELTKEGEL 496
            MN+SL KS  + N S SK        REEYHLTP++G++RSSTVLLNG  LE TKEG++
Sbjct: 446 DMNMSLRKSNTRTNGSHSKYRQKRTASREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDI 505

BLAST of PI0008465 vs. NCBI nr
Match: XP_004140375.1 (heparanase-like protein 1 [Cucumis sativus] >KAE8648411.1 hypothetical protein Csa_008460 [Cucumis sativus])

HSP 1 Score: 842.4 bits (2175), Expect = 2.0e-240
Identity = 416/520 (80.00%), Postives = 443/520 (85.19%), Query Frame = 0

Query: 24  FCFHPKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASM 83
           F F P+   G NVT GKIVVDGTTKI+ETDENFICFTLDIW HDECSQPNLC WDGHASM
Sbjct: 12  FAFIPRTILGLNVTTGKIVVDGTTKIAETDENFICFTLDIWPHDECSQPNLCVWDGHASM 71

Query: 84  LNLDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEG 143
           LN+DLSLPILNKAV++FKTL+IRVGGTLQDRLIY+IGDGFKGNC+PF+A K LLFDFTEG
Sbjct: 72  LNMDLSLPILNKAVQAFKTLRIRVGGTLQDRLIYNIGDGFKGNCNPFEAHKGLLFDFTEG 131

Query: 144 CLYMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKY 203
           CLYMERWDDLN FFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNY NAEAL+KYTV+KKY
Sbjct: 132 CLYMERWDDLNNFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYSNAEALIKYTVEKKY 191

Query: 204 NINSWEFGN-----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDK 263
           NINSWEFGN           V+ASQYAK+LLKLR+IIDRLYKNSQQKPLIVAPGAFFDDK
Sbjct: 192 NINSWEFGNELAGPNSIGASVSASQYAKDLLKLRQIIDRLYKNSQQKPLIVAPGAFFDDK 251

Query: 264 WYDELVTKTGSNVVSTLTHHIYIYNMGA------------------VSNTFRQLKNIIEK 323
           WYDELVTKTGSNVVS LTHH  IYNMGA                  VSNTFRQLKNIIEK
Sbjct: 252 WYDELVTKTGSNVVSALTHH--IYNMGAGDDPKLIYRFVNPTYLSQVSNTFRQLKNIIEK 311

Query: 324 HAPWASAWVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNI 383
           HAPWASAWVGEAGGAYHGG  HISD FINSFW                  QTL+GG+Y +
Sbjct: 312 HAPWASAWVGEAGGAYHGGGLHISDTFINSFWYLDQLGMAASYNTKVYCRQTLVGGYYGV 371

Query: 384 LRAKTYIPTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNT 443
           LR KT+IPT DYYGALLFHRLMGSS LKVDNNVSSYLRTYAHCSRG+SGVTMLFINLSNT
Sbjct: 372 LRTKTFIPTPDYYGALLFHRLMGSSVLKVDNNVSSYLRTYAHCSRGRSGVTMLFINLSNT 431

Query: 444 TKFTIKIENHMNLSLHKSKPKNSSPSKNVGTQREEYHLTPENGIIRSSTVLLNGRELELT 497
           T+FTI IENHMNLSLHKSKPK+SS SKNVGTQREEYHLTP+NG++RSSTVLLNG+ LELT
Sbjct: 432 TEFTINIENHMNLSLHKSKPKHSS-SKNVGTQREEYHLTPQNGLLRSSTVLLNGKALELT 491

BLAST of PI0008465 vs. NCBI nr
Match: KAA0044764.1 (heparanase-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 783.1 bits (2021), Expect = 1.4e-222
Identity = 382/503 (75.94%), Postives = 428/503 (85.09%), Query Frame = 0

Query: 26  FHPKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLN 85
           F P+   GHNVT GKIVVDGTTKI+ETDENFICFTLDIW HDECSQPNLC WD HAS+LN
Sbjct: 14  FIPRTILGHNVTTGKIVVDGTTKIAETDENFICFTLDIWPHDECSQPNLCVWDSHASVLN 73

Query: 86  LDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCL 145
           LDLSLPILNKAV++FKTL+IRVGGTLQDRLIY+IG+GF+GNCHPF+AD  LLF+FTEGCL
Sbjct: 74  LDLSLPILNKAVQAFKTLRIRVGGTLQDRLIYNIGEGFEGNCHPFEADDSLLFEFTEGCL 133

Query: 146 YMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNI 205
           YMERWDDLNKFFNNTGA+VTFGLNALLGKY+TKG+QWEGNWN+ NAEAL+KYTVD  Y I
Sbjct: 134 YMERWDDLNKFFNNTGALVTFGLNALLGKYHTKGMQWEGNWNHTNAEALIKYTVDNNYQI 193

Query: 206 NSWEFGN-----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWY 265
           NSWEFGN           V+A+QYAK+LLKLRE++DRLY+NSQQKP+IVAPGAFFDDKWY
Sbjct: 194 NSWEFGNELGGANSIGASVSAAQYAKDLLKLREMVDRLYENSQQKPMIVAPGAFFDDKWY 253

Query: 266 DELVTKTGSNVVSTLTHHIYIYNMGAVSNTFRQLKNIIEKHAPWASAWVGEAGGAYHGGS 325
            ELVTKTG NVV+ LTHH  IYNM  VS TFRQLKNI+EKHAPW+SAWVGEAGGAYHGG+
Sbjct: 254 HELVTKTGPNVVTALTHH--IYNM--VSKTFRQLKNIVEKHAPWSSAWVGEAGGAYHGGA 313

Query: 326 PHISDAFINSFW------------------QTLIGGFYNILRAKTYIPTLDYYGALLFHR 385
             ISD FINSFW                  QTL+GGFY++L+AKT++PT DYYGALLFHR
Sbjct: 314 YRISDTFINSFWYLDQLGMASLYNTKVYCRQTLVGGFYSVLKAKTFVPTPDYYGALLFHR 373

Query: 386 LMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKIENHMNLSLHK-SK 445
           LMG   LKV N VS YLRTYAHCSR KSGVTMLFINLSNTT+FTI +E+ MNLSLHK  K
Sbjct: 374 LMGPGVLKVYNEVSPYLRTYAHCSREKSGVTMLFINLSNTTEFTINVEDPMNLSLHKRGK 433

Query: 446 PK-NSSPSKNVG-TQREEYHLTPENGIIRSSTVLLNGRELELTKEGELPDLVPVYRDSNS 497
           P  +SS + NVG T+REEYHLTP+NG++RSSTVLLNG+ LELTKEGELPDL P+YRDSNS
Sbjct: 434 PNYDSSSNDNVGTTEREEYHLTPQNGLLRSSTVLLNGKALELTKEGELPDLTPIYRDSNS 493

BLAST of PI0008465 vs. NCBI nr
Match: KAA0044757.1 (heparanase-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 776.9 bits (2005), Expect = 1.0e-220
Identity = 380/511 (74.36%), Postives = 429/511 (83.95%), Query Frame = 0

Query: 26  FHPKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLN 85
           F P+   G+NVTIGKIVVDGTTKI+ETDENFICFTLDIW HDECSQPNLC WD HAS+LN
Sbjct: 14  FIPRTILGYNVTIGKIVVDGTTKIAETDENFICFTLDIWPHDECSQPNLCVWDSHASVLN 73

Query: 86  LDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCL 145
           LDLSLPILNKAV++FKTL+IRVGGTLQDRLIY+IG+GF+GNCHPF+AD  LLF+FTEGCL
Sbjct: 74  LDLSLPILNKAVQAFKTLRIRVGGTLQDRLIYNIGEGFEGNCHPFEADDSLLFEFTEGCL 133

Query: 146 YMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNI 205
           YMERWDDLNKFFNNTGA+VTFGLNALLGKY+TKG+QWEGNWN+ NAEAL+KYTVD  Y I
Sbjct: 134 YMERWDDLNKFFNNTGALVTFGLNALLGKYHTKGMQWEGNWNHTNAEALIKYTVDNNYQI 193

Query: 206 NSWEFGN-----VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDELVTK 265
           NSWEF N     V+A+QYAK+LLKLRE++DRLY+NSQQKP+IVAPGAFFDDKWY ELVTK
Sbjct: 194 NSWEFANSIGASVSAAQYAKDLLKLREMVDRLYENSQQKPMIVAPGAFFDDKWYHELVTK 253

Query: 266 TGSNVVSTLTHHIY--------IYN------MGAVSNTFRQLKNIIEKHAPWASAWVGEA 325
           TG NVV+ LTHHIY        IY       +  VS TFRQLKNI+EKHAPW+SAWVGEA
Sbjct: 254 TGPNVVTALTHHIYNMGDDPKLIYRFVNPTYLSEVSKTFRQLKNIVEKHAPWSSAWVGEA 313

Query: 326 GGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTYIPTLDY 385
           GGAYHGG+  ISD FINSFW                  QTL+GGFY++L+AKT++PT DY
Sbjct: 314 GGAYHGGAYRISDTFINSFWYLDQLGMASLYNTKVYCRQTLVGGFYSVLKAKTFVPTPDY 373

Query: 386 YGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKIENHMN 445
           YGALLFHRLMG   LKV N VS YLRTYAHCSR KSGVTMLFINLSNTT+FTI +E+ +N
Sbjct: 374 YGALLFHRLMGPGVLKVYNEVSPYLRTYAHCSREKSGVTMLFINLSNTTEFTINVEDPVN 433

Query: 446 LSLHK-SKPK-NSSPSKNVGT-QREEYHLTPENGIIRSSTVLLNGRELELTKEGELPDLV 497
           LSLHK  KP  +SS + NVGT +REEYHLTP+NG++RSSTVLLNG+ LELTKEGELPDL 
Sbjct: 434 LSLHKRGKPNYDSSSNDNVGTIEREEYHLTPQNGLLRSSTVLLNGKALELTKEGELPDLT 493

BLAST of PI0008465 vs. NCBI nr
Match: KGN53270.1 (hypothetical protein Csa_015114 [Cucumis sativus])

HSP 1 Score: 768.5 bits (1983), Expect = 3.6e-218
Identity = 373/520 (71.73%), Postives = 425/520 (81.73%), Query Frame = 0

Query: 26  FHPKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLN 85
           F P+   G NVT+GKIVV+G TKI+ETDENFICFTLDIW HDECSQPNLC WD HAS+LN
Sbjct: 14  FIPRTILGQNVTMGKIVVEGITKIAETDENFICFTLDIWPHDECSQPNLCVWDSHASVLN 73

Query: 86  LDLSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCL 145
           +DLSLPI+NKAV++FKTL+IRVGGTLQDRLIY+IG+GFKGNCHPF+AD  LLFDFTEGCL
Sbjct: 74  VDLSLPIINKAVQAFKTLRIRVGGTLQDRLIYNIGEGFKGNCHPFEADDSLLFDFTEGCL 133

Query: 146 YMERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNI 205
           YMERWDDLN FFNNTGAIVTFGLNALLGKY+T+G+QWEGNWNY NAEAL+KYTVDK Y I
Sbjct: 134 YMERWDDLNNFFNNTGAIVTFGLNALLGKYHTQGMQWEGNWNYTNAEALIKYTVDKNYQI 193

Query: 206 NSWEFGN-----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWY 265
           NSWEFGN           ++ASQYAK+LLKLREI+DRLYKNSQQKPLIVAPGAFFDDKWY
Sbjct: 194 NSWEFGNELAGRNSIGASISASQYAKDLLKLREIVDRLYKNSQQKPLIVAPGAFFDDKWY 253

Query: 266 DELVTKTGSNVVSTLTHHIYIYNMGA------------------VSNTFRQLKNIIEKHA 325
            ELVTKTG  VVS LTHH  IYNMGA                  VSNTF+QLKNI++KHA
Sbjct: 254 HELVTKTGPKVVSVLTHH--IYNMGAGDDPKLIYRFVNPTYLSQVSNTFKQLKNIVQKHA 313

Query: 326 PWASAWVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILR 385
           PW+SAWVGEAGGAY GG+  ISD+FINSFW                  QTLIGGFY++L+
Sbjct: 314 PWSSAWVGEAGGAYQGGAYRISDSFINSFWYLDQLGMAAFYNTKVYCRQTLIGGFYSVLK 373

Query: 386 AKTYIPTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTK 445
           AKT +PT DYYGALLFHRLMG   LKV N VS+YLRTYAHCSR +SG++MLFINLSNTT+
Sbjct: 374 AKTLVPTPDYYGALLFHRLMGPGVLKVHNKVSTYLRTYAHCSRERSGISMLFINLSNTTE 433

Query: 446 FTIKIENHMNLSLHK-SKPKNSSPS-KNVGTQREEYHLTPENGIIRSSTVLLNGRELELT 497
           F I +++HM LSLHK  KPK+ S S  N+GT REEYHLTP+NG++RSS VLLNG+ L+LT
Sbjct: 434 FAINVKDHMTLSLHKRRKPKHGSSSINNLGTPREEYHLTPQNGLLRSSNVLLNGKALQLT 493

BLAST of PI0008465 vs. NCBI nr
Match: XP_038877281.1 (LOW QUALITY PROTEIN: heparanase-like protein 1 [Benincasa hispida])

HSP 1 Score: 741.1 bits (1912), Expect = 6.2e-210
Identity = 362/511 (70.84%), Postives = 413/511 (80.82%), Query Frame = 0

Query: 33  GHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLDLSLPI 92
           GHNV  GKIVVD TTKI+ETDENFICFT+DIW HDECSQPNLC WD HAS+LN+DLSLP+
Sbjct: 20  GHNVAKGKIVVDETTKIAETDENFICFTMDIWPHDECSQPNLCVWDDHASILNVDLSLPM 79

Query: 93  LNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYMERWDD 152
           LNKAV++FK+L+IR+GGTLQDRLIY++G+GFK +C PFQAD DLLFDF+EGCLYMERWDD
Sbjct: 80  LNKAVQAFKSLRIRIGGTLQDRLIYNVGEGFKSHCQPFQADNDLLFDFSEGCLYMERWDD 139

Query: 153 LNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINSWEFG- 212
           LN FFNNTGAI+TFGLNALLGK+NTKG+QWEGNWNY NAEAL++YTVDK Y INSWEFG 
Sbjct: 140 LNNFFNNTGAIITFGLNALLGKHNTKGMQWEGNWNYSNAEALIQYTVDKNYQINSWEFGK 199

Query: 213 ----------NVNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDELVTKT 272
                     +V+ASQYAK+L+KLREIIDRLY NSQQKPL+VAPGAFFDDKWY ELV K 
Sbjct: 200 LMGGRNSIGASVSASQYAKDLMKLREIIDRLYNNSQQKPLMVAPGAFFDDKWYHELVKKN 259

Query: 273 GSNVVSTLTHHIYIYNMGA------------------VSNTFRQLKNIIEKHAPWASAWV 332
            SN+V+ LTHH  IYNMG                   VSNTF+QL NII+KHAPWASAWV
Sbjct: 260 DSNIVNALTHH--IYNMGTGDDPKLIYRFVNPTYLSQVSNTFKQLDNIIQKHAPWASAWV 319

Query: 333 GEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTYIPT 392
           GEAGGAYHGGSPHISDAFINSFW                  QTLIGGFY++L++KTY+PT
Sbjct: 320 GEAGGAYHGGSPHISDAFINSFWYLDQLGMASFYNTKVYCRQTLIGGFYSVLKSKTYLPT 379

Query: 393 LDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKIEN 452
            DYYGALLFHRLMGS  LKVDNNVSSYLRTYAHCSRG+SGVT+LFINLSNTT+FTIKIE+
Sbjct: 380 PDYYGALLFHRLMGSRVLKVDNNVSSYLRTYAHCSRGRSGVTLLFINLSNTTEFTIKIES 439

Query: 453 HMNLSLHKSKPKNSSPSK-NVGTQREEYHLTPENGIIRSSTVLLNGRELELTKEGELPDL 496
           HMN SLH S  +  + S     ++ ++ +L  +NG++RSSTVLLN   LELTKEGELP+ 
Sbjct: 440 HMNPSLHNSNSRRPNDSNLKYSSKVKDPNLPTQNGLLRSSTVLLNENPLELTKEGELPNF 499

BLAST of PI0008465 vs. TAIR 10
Match: AT5G07830.1 (glucuronidase 2 )

HSP 1 Score: 466.5 bits (1199), Expect = 2.7e-131
Identity = 231/528 (43.75%), Postives = 333/528 (63.07%), Query Frame = 0

Query: 28  PKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLD 87
           P+      +    IV+ G  ++ ETDENF+C TLD W HD+C+  + C W G++S++N+D
Sbjct: 19  PEKTMAQEMKRASIVIQGARRVCETDENFVCATLDWWPHDKCNY-DQCPW-GYSSVINMD 78

Query: 88  LSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYM 147
           L+ P+L KA+++FK L+IR+GG+LQD++IYD+G+  K  C PFQ     LF F++GCL+M
Sbjct: 79  LTRPLLTKAIKAFKPLRIRIGGSLQDQVIYDVGN-LKTPCRPFQKMNSGLFGFSKGCLHM 138

Query: 148 ERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINS 207
           +RWD+LN F   TGA+VTFGLNAL G++  +G  W G W++ N +  + YTV K Y I+S
Sbjct: 139 KRWDELNSFLTATGAVVTFGLNALRGRHKLRGKAWGGAWDHINTQDFLNYTVSKGYVIDS 198

Query: 208 WEFGN----------VNASQYAKNLLKLREIIDRLYKNS-QQKPLIVAPGAFFDDKWYDE 267
           WEFGN          V+A  Y K+L+ L+++I+++YKNS   KP++VAPG F++ +WY +
Sbjct: 199 WEFGNELSGSGVGASVSAELYGKDLIVLKDVINKVYKNSWLHKPILVAPGGFYEQQWYTK 258

Query: 268 LVTKTGSNVVSTLTHHIYIYNMG----------------AVSNTFRQLKNIIEKHAPWAS 327
           L+  +G +VV  +THHIY    G                 VS TF+ +   I++H PWAS
Sbjct: 259 LLEISGPSVVDVVTHHIYNLGSGNDPALVKKIMDPSYLSQVSKTFKDVNQTIQEHGPWAS 318

Query: 328 AWVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTY 387
            WVGE+GGAY+ G  H+SD FI+SFW                  QTL+GGFY +L   T+
Sbjct: 319 PWVGESGGAYNSGGRHVSDTFIDSFWYLDQLGMSARHNTKVYCRQTLVGGFYGLLEKGTF 378

Query: 388 IPTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIK 447
           +P  DYY ALL+HRLMG   L V  +    LR YAHCS+G++GVT+L INLSN + FT+ 
Sbjct: 379 VPNPDYYSALLWHRLMGKGVLAVQTDGPPQLRVYAHCSKGRAGVTLLLINLSNQSDFTVS 438

Query: 448 IENHMNLSLHKSKPKNSS-------PSKNVGTQ-------REEYHLTPENGIIRSSTVLL 497
           + N +N+ L+    K  S       P   +G++       REEYHLTPENG++RS T++L
Sbjct: 439 VSNGINVVLNAESRKKKSLLDTLKRPFSWIGSKASDGYLNREEYHLTPENGVLRSKTMVL 498

BLAST of PI0008465 vs. TAIR 10
Match: AT5G61250.2 (glucuronidase 1 )

HSP 1 Score: 453.4 bits (1165), Expect = 2.4e-127
Identity = 230/527 (43.64%), Postives = 322/527 (61.10%), Query Frame = 0

Query: 28  PKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLD 87
           P   FG N+    +V+DG+ +I+ETDENFIC TLD W  ++C+  + C W G+AS++NL+
Sbjct: 16  PPVTFGSNMERTTLVIDGSRRIAETDENFICATLDWWPPEKCNY-DQCPW-GYASLINLN 75

Query: 88  LSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYM 147
           L+ P+L KA+++F+TL+IR+GG+LQD++IYD+GD  K  C  F+   D LF F+EGCLYM
Sbjct: 76  LASPLLAKAIQAFRTLRIRIGGSLQDQVIYDVGD-LKTPCTQFKKTDDGLFGFSEGCLYM 135

Query: 148 ERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINS 207
           +RWD++N FFN TGAIVTFGLNAL G+    G  W G+W++ N +  + YTV K Y I+S
Sbjct: 136 KRWDEVNHFFNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTVSKGYAIDS 195

Query: 208 WEFGN----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDEL 267
           WEFGN          V+   Y K+L+ L+ +I  +YKNS+ KPL+VAPG FF+++WY EL
Sbjct: 196 WEFGNELSGSGIWASVSVELYGKDLIVLKNVIKNVYKNSRTKPLVVAPGGFFEEQWYSEL 255

Query: 268 VTKTGSNVVSTLTHHIYIYNMG----------------AVSNTFRQLKNIIEKHAPWASA 327
           +  +G  V+  LTHHIY    G                 +S  F  +   I++H PWA+A
Sbjct: 256 LRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGISELFANVNQTIQEHGPWAAA 315

Query: 328 WVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTYI 387
           WVGEAGGA++ G   +S+ FINSFW                  Q L+GGFY +L  +T++
Sbjct: 316 WVGEAGGAFNSGGRQVSETFINSFWYLDQLGISSKHNTKVYCRQALVGGFYGLLEKETFV 375

Query: 388 PTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKI 447
           P  DYY ALL+HRLMG   L V    S YLR Y HCS+ ++G+T+L INLS  T FT+ +
Sbjct: 376 PNPDYYSALLWHRLMGKGILGVQTTASEYLRAYVHCSKRRAGITILLINLSKHTTFTVAV 435

Query: 448 ENHMNLSLHKSKPKNSSPSKNVGTQ--------------REEYHLTPENGIIRSSTVLLN 497
            N + + L     K  S  + + ++              REEYHL+P++G +RS  +LLN
Sbjct: 436 SNGVKVVLQAESMKRKSFLETIKSKVSWVGNKASDGYLNREEYHLSPKDGDLRSKIMLLN 495

BLAST of PI0008465 vs. TAIR 10
Match: AT5G61250.1 (glucuronidase 1 )

HSP 1 Score: 453.4 bits (1165), Expect = 2.4e-127
Identity = 230/527 (43.64%), Postives = 322/527 (61.10%), Query Frame = 0

Query: 28  PKNYFGHNVTIGKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLD 87
           P   FG N+    +V+DG+ +I+ETDENFIC TLD W  ++C+  + C W G+AS++NL+
Sbjct: 16  PPVTFGSNMERTTLVIDGSRRIAETDENFICATLDWWPPEKCNY-DQCPW-GYASLINLN 75

Query: 88  LSLPILNKAVRSFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYM 147
           L+ P+L KA+++F+TL+IR+GG+LQD++IYD+GD  K  C  F+   D LF F+EGCLYM
Sbjct: 76  LASPLLAKAIQAFRTLRIRIGGSLQDQVIYDVGD-LKTPCTQFKKTDDGLFGFSEGCLYM 135

Query: 148 ERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINS 207
           +RWD++N FFN TGAIVTFGLNAL G+    G  W G+W++ N +  + YTV K Y I+S
Sbjct: 136 KRWDEVNHFFNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMNYTVSKGYAIDS 195

Query: 208 WEFGN----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDEL 267
           WEFGN          V+   Y K+L+ L+ +I  +YKNS+ KPL+VAPG FF+++WY EL
Sbjct: 196 WEFGNELSGSGIWASVSVELYGKDLIVLKNVIKNVYKNSRTKPLVVAPGGFFEEQWYSEL 255

Query: 268 VTKTGSNVVSTLTHHIYIYNMG----------------AVSNTFRQLKNIIEKHAPWASA 327
           +  +G  V+  LTHHIY    G                 +S  F  +   I++H PWA+A
Sbjct: 256 LRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGISELFANVNQTIQEHGPWAAA 315

Query: 328 WVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTYI 387
           WVGEAGGA++ G   +S+ FINSFW                  Q L+GGFY +L  +T++
Sbjct: 316 WVGEAGGAFNSGGRQVSETFINSFWYLDQLGISSKHNTKVYCRQALVGGFYGLLEKETFV 375

Query: 388 PTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKI 447
           P  DYY ALL+HRLMG   L V    S YLR Y HCS+ ++G+T+L INLS  T FT+ +
Sbjct: 376 PNPDYYSALLWHRLMGKGILGVQTTASEYLRAYVHCSKRRAGITILLINLSKHTTFTVAV 435

Query: 448 ENHMNLSLHKSKPKNSSPSKNVGTQ--------------REEYHLTPENGIIRSSTVLLN 497
            N + + L     K  S  + + ++              REEYHL+P++G +RS  +LLN
Sbjct: 436 SNGVKVVLQAESMKRKSFLETIKSKVSWVGNKASDGYLNREEYHLSPKDGDLRSKIMLLN 495

BLAST of PI0008465 vs. TAIR 10
Match: AT5G34940.2 (glucuronidase 3 )

HSP 1 Score: 366.3 bits (939), Expect = 3.9e-101
Identity = 201/509 (39.49%), Postives = 285/509 (55.99%), Query Frame = 0

Query: 39  GKIVVDGTTKISETDENFICFTLDIWSHDECSQPNLCAWDGHASMLNLDLSLPILNKAVR 98
           G + V G   +   DE+FIC TLD W  ++C   + C+WD HAS+LNLDL+  IL  A++
Sbjct: 31  GTVFVYGRAAVGTIDEDFICATLDWWPPEKCDYGS-CSWD-HASILNLDLNNVILQNAIK 90

Query: 99  SFKTLKIRVGGTLQDRLIYDIGDGFKGNCHPFQADKDLLFDFTEGCLYMERWDDLNKFFN 158
           +F  LKIR+GGTLQD +IY+  D  K  C PF  +  +LF +T+GCL M RWD+LN FF 
Sbjct: 91  AFAPLKIRIGGTLQDIVIYETPDS-KQPCLPFTKNSSILFGYTQGCLPMRRWDELNAFFR 150

Query: 159 NTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNINSWEFGN------ 218
            TG  V FGLNAL G+      +  G WNY NAE+ +++T +  Y I+ WE GN      
Sbjct: 151 KTGTKVIFGLNALSGRSIKSNGEAIGAWNYTNAESFIRFTAENNYTIDGWELGNELCGSG 210

Query: 219 ----VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDELVTKTGSNVVST 278
               V A+QYA + + LR I++R+YKN    PL++ PG FF+  W+ E + K   N ++ 
Sbjct: 211 VGARVGANQYAIDTINLRNIVNRVYKNVSPMPLVIGPGGFFEVDWFTEYLNK-AENSLNA 270

Query: 279 LTHHIYIYNMGA----------------VSNTFRQLKNIIEKHAPWASAWVGEAGGAYHG 338
            T HIY    G                  + +FR LKNII+  +  A AWVGE+GGAY+ 
Sbjct: 271 TTRHIYDLGPGVDEHLIEKILNPSYLDQEAKSFRSLKNIIKNSSTKAVAWVGESGGAYNS 330

Query: 339 GSPHISDAFINSFW------------------QTLIGGFYNILRAKTYIPTLDYYGALLF 398
           G   +S+AF+ SFW                  Q+LIGG Y +L    + P  DYY AL++
Sbjct: 331 GRNLVSNAFVYSFWYLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTNFTPNPDYYSALIW 390

Query: 399 HRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIKIENHMNLSLHKS 458
            +LMG  AL    + +  +R+Y HC+R   G+T+L +NL NTT    K+E + + SL  +
Sbjct: 391 RQLMGRKALFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNSFSLRHT 450

Query: 459 KPKNSSP--------SKNVGTQREEYHLTPENGIIRSSTVLLNGRELELTKEGELPDLVP 496
           K   S            N   QREEYHLT ++G + S T+LLNG  L++   G+LP + P
Sbjct: 451 KHMKSYKRASSQLFGGPNGVIQREEYHLTAKDGNLHSQTMLLNGNALQVNSMGDLPPIEP 510

BLAST of PI0008465 vs. TAIR 10
Match: AT5G34940.1 (glucuronidase 3 )

HSP 1 Score: 268.9 bits (686), Expect = 8.4e-72
Identity = 150/401 (37.41%), Postives = 215/401 (53.62%), Query Frame = 0

Query: 147 MERWDDLNKFFNNTGAIVTFGLNALLGKYNTKGIQWEGNWNYRNAEALVKYTVDKKYNIN 206
           M RWD+LN FF  TG  V FGLNAL G+      +  G WNY NAE+ +++T +  Y I+
Sbjct: 1   MRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAWNYTNAESFIRFTAENNYTID 60

Query: 207 SWEFGN----------VNASQYAKNLLKLREIIDRLYKNSQQKPLIVAPGAFFDDKWYDE 266
            WE GN          V A+QYA + + LR I++R+YKN    PL++ PG FF+  W+ E
Sbjct: 61  GWELGNELCGSGVGARVGANQYAIDTINLRNIVNRVYKNVSPMPLVIGPGGFFEVDWFTE 120

Query: 267 LVTKTGSNVVSTLTHHIYIYNMGA----------------VSNTFRQLKNIIEKHAPWAS 326
            + K   N ++  T HIY    G                  + +FR LKNII+  +  A 
Sbjct: 121 YLNK-AENSLNATTRHIYDLGPGVDEHLIEKILNPSYLDQEAKSFRSLKNIIKNSSTKAV 180

Query: 327 AWVGEAGGAYHGGSPHISDAFINSFW------------------QTLIGGFYNILRAKTY 386
           AWVGE+GGAY+ G   +S+AF+ SFW                  Q+LIGG Y +L    +
Sbjct: 181 AWVGESGGAYNSGRNLVSNAFVYSFWYLDQLGMASLYDTKTYCRQSLIGGNYGLLNTTNF 240

Query: 387 IPTLDYYGALLFHRLMGSSALKVDNNVSSYLRTYAHCSRGKSGVTMLFINLSNTTKFTIK 446
            P  DYY AL++ +LMG  AL    + +  +R+Y HC+R   G+T+L +NL NTT    K
Sbjct: 241 TPNPDYYSALIWRQLMGRKALFTTFSGTKKIRSYTHCARQSKGITVLLMNLDNTTTVVAK 300

Query: 447 IENHMNLSLHKSKPKNSSP--------SKNVGTQREEYHLTPENGIIRSSTVLLNGRELE 496
           +E + + SL  +K   S            N   QREEYHLT ++G + S T+LLNG  L+
Sbjct: 301 VELNNSFSLRHTKHMKSYKRASSQLFGGPNGVIQREEYHLTAKDGNLHSQTMLLNGNALQ 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FF103.9e-13043.75Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1[more]
Q8L6083.4e-12643.64Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1[more]
Q9FZP15.4e-10039.49Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2[more]
Q9LRC83.8e-8536.58Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 S... [more]
X4Y2L46.3e-2425.54Hyaluronoglucuronidase OS=Hirudo nipponia OX=42736 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KTJ91.9e-24178.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G390000 PE=3 SV=1[more]
A0A5A7TNQ56.9e-22375.94Heparanase-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A5A7TSB34.9e-22174.36Heparanase-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A0A0KUF11.7e-21871.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G043860 PE=3 SV=1[more]
A0A6J1I2E32.1e-19566.15heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111470271 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004140375.12.0e-24080.00heparanase-like protein 1 [Cucumis sativus] >KAE8648411.1 hypothetical protein C... [more]
KAA0044764.11.4e-22275.94heparanase-like protein 1 [Cucumis melo var. makuwa][more]
KAA0044757.11.0e-22074.36heparanase-like protein 1 [Cucumis melo var. makuwa][more]
KGN53270.13.6e-21871.73hypothetical protein Csa_015114 [Cucumis sativus][more]
XP_038877281.16.2e-21070.84LOW QUALITY PROTEIN: heparanase-like protein 1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G07830.12.7e-13143.75glucuronidase 2 [more]
AT5G61250.22.4e-12743.64glucuronidase 1 [more]
AT5G61250.12.4e-12743.64glucuronidase 1 [more]
AT5G34940.23.9e-10139.49glucuronidase 3 [more]
AT5G34940.18.4e-7237.41glucuronidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 76..356
e-value: 8.6E-44
score: 152.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 411..431
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 412..431
NoneNo IPR availablePANTHERPTHR14363HEPARANASE-RELATEDcoord: 327..496
NoneNo IPR availablePANTHERPTHR14363HEPARANASE-RELATEDcoord: 29..326
NoneNo IPR availablePANTHERPTHR14363:SF21HEPARANASE-LIKE PROTEIN 1coord: 29..326
NoneNo IPR availablePANTHERPTHR14363:SF21HEPARANASE-LIKE PROTEIN 1coord: 327..496
IPR005199Glycoside hydrolase, family 79PFAMPF03662Glyco_hydro_79ncoord: 40..326
e-value: 4.7E-103
score: 344.5
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 53..358

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0008465.1PI0008465.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0004566 beta-glucuronidase activity
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds