PI0007927 (gene) Melon (PI 482460) v1

Overview


Name	PI0007927
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	DNA-directed RNA polymerases II and IV subunit 5A-like
Location	chr12: 10060794 .. 10063149 (+)
RNA-Seq Expression	PI0007927
Synteny	PI0007927

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATCACAAATATTAGTGATGTTTTGCTATACTTTAATATTTTTTATTATTTCGTTATATTAATTTAGAGCATAAATTATGCAATGTGAACTTAAACATTTTCTAAAAAATTTGAAAGTTTGTTCGGTGTTTGAACTCCCATTAAAAACCAAAATACTTGGGTAAACCAAGATTTCTTTCTTTCACTTTCCTTCTTAATCAGCTTCTTTCAGTACTTTTTCCTCTTAGGAACAAATTAGTTCAATTCTCGTTTTCCGGTTTGTGATTTTGTTTATATTTCAATCATGATGCTGATTTTTTTTCCTGTTGTCTGGATTATGTTTTAGGATTATATTAATGAAGTTGTTGAGGGGAATATGGATAGATTTTATTTTCTTTCTCCCCCTGTTTTTGATTTGGGAAATTGAGTTTTATACTCTTCCATGTGCATCTTTTTGAGAAATTAGAATAATAATTGAATACTGGATTTCTTGAAAGTGTTTTTAGGCTGAAAGAGATGGTGTTGTCAGAGGAAGAAATCACCAGACTCTTTAGAGTCCGCAAAACTGTGTTGCAGATGTTGAGAGACAGAGGCTACATTGTTGGAGACTTTGAGTTTGCTATGTCAAGAGAACAATTTAAAAATCAGTATGGTGAGACCATGAAAAAAGAAGATCTTGTTATCAATAAATCCATGAGAAATAATAGCCCTAACCAGGTATAGTCTCATGCTAGATCGTGTCTCCAAACTTGTTTACTTTGATTCTTACTTAGCAGCTCCATCTTACAACTCTGCACCCAAACACGGGATTCCTAATTCAATTCTAACTTATTAACTCAAACTTCTAACTCAATTTAGTGCCTCCAACACCCCTCAATTGTATAAAACAAGGTCTCAGTGACTCATGCAATTATTGTATATTTGAACACGTTTAAACTTTACCTTGATAAATTTCCTTAGGAGAAATTTCTATTTGAGTCATTCTCAGATACTTTGACATGGAAATTACTTAACTATCTATCATTGAGTAGTATATTATACTATTTGATAAATATCTACCAATTTTATGTTAAGTTTGGTTATAGGGATGTACAGGTTATTTGTTAATCCTTTTATTTGTTCCCCACTAATTTATGACTCTTTTACACAGATATATGTTTTCTTTCCTGAAGAGCCGAAGGTTAGTGTCAAGACAGTGCGAACATATGTAAATCGTATGAAATCAGATAATGTTTTTAGAGCAATATTGGTTGTTCAAGATAAATTGTCCTCCTTTGCTCGTTCTTGTATCTGCGAAGCACCTAAATTCCATTTGGAGGTTTTCCAGGTAAAAATTAAATCTCTGAATAATTTTTGGAGCCTTTTAATGTTCAGATAGATTATAGTAATATTATCGCCATCACATGCTACAAATTTGATGAGAACTAGAGAGGATCTTCTATGCTCCTATCCTAAAATTAGTTCTCATACTTTTATTGTCTCTCACATCGACAGTGAGAATGAAGGGGGGCGGGGAGGGGGGGGGGTGGTAAAGAATGGTATTTTAGTTTTTAATTACTTGTGTGATTTCCATTTGTCATATAATAATGCTTTGCCTACACTTTTTTTTTTTTCTGAAGTTAATATTTACCTCTTTGTCACTTTTTGATGCAGGAAGCAGAATTGCTGGTTAACATCAAAGATCACGTTCTCATTCCCGAGCACCAGTTGTTGACAAACGAAGAGAAGAAGACGTTGTGGAGAGGTATACGGTGAAAGATACACAAGTGAGTTTCATTTTATTAGATCTATTTTCTTGAAATACAAGAAACATTGTGAGAATTATCTTCCATAAATATTTTGAAATGACCTTGGTAGATTGGCCTGCGGTGAAGAAGGGCTTTGTAAATAATAAAAATTTTATGAATGAGCTCAAGCCATTATGAGATTTAATATTCTCCGAGTTACATTGGCCATCAAATATAGTCGGGTTAGGCAGTTCGTTAGAATAGTCGCGATGCATGCAACTTGGTTCTGACATTCTTGGATACATATTGAAATGTGTATAGGTTAGTTCCTAATGTGACTTCGTATTTTGTCTTAGCTACCTCGCATGCAGGTTACTGATCCAATTGCTAGATATTATGGAATGAAGCGTGGACAAGTAGTGAAGATCATCCGACCAAGCGAAACTGCGGGAAGATATGTGACATATAGATATGTTGTTTGAGATCAATATGATATGAGCGATTGAAGTCATGGTACTTGCCTATTTGGAAGTGTGGAAGTGGTTTTAGATTTAGGCAATTCTCGTATATGAGCTCAAACTAGGGAATTCTTTTATTTAGAACATTCTTTTATTTAGTAGCTTCTCTGTGTTCCTTCATCTTTATCTTCT

mRNA sequence

TATCACAAATATTAGTGATGTTTTGCTATACTTTAATATTTTTTATTATTTCGTTATATTAATTTAGAGCATAAATTATGCAATGTGAACTTAAACATTTTCTAAAAAATTTGAAAGTTTGTTCGGTGTTTGAACTCCCATTAAAAACCAAAATACTTGGGTAAACCAAGATTTCTTTCTTTCACTTTCCTTCTTAATCAGCTTCTTTCAGTACTTTTTCCTCTTAGGAACAAATTAGTTCAATTCTCGTTTTCCGGTTTGTGATTTTGTTTATATTTCAATCATGATGCTGATTTTTTTTCCTGTTGTCTGGATTATGTTTTAGGATTATATTAATGAAGTTGTTGAGGGGAATATGGATAGATTTTATTTTCTTTCTCCCCCTGTTTTTGATTTGGGAAATTGAGTTTTATACTCTTCCATGTGCATCTTTTTGAGAAATTAGAATAATAATTGAATACTGGATTTCTTGAAAGTGTTTTTAGGCTGAAAGAGATGGTGTTGTCAGAGGAAGAAATCACCAGACTCTTTAGAGTCCGCAAAACTGTGTTGCAGATGTTGAGAGACAGAGGCTACATTGTTGGAGACTTTGAGTTTGCTATGTCAAGAGAACAATTTAAAAATCAGTATGGTGAGACCATGAAAAAAGAAGATCTTGTTATCAATAAATCCATGAGAAATAATAGCCCTAACCAGATATATGTTTTCTTTCCTGAAGAGCCGAAGGTTAGTGTCAAGACAGTGCGAACATATGTAAATCGTATGAAATCAGATAATGTTTTTAGAGCAATATTGGTTGTTCAAGATAAATTGTCCTCCTTTGCTCGTTCTTGTATCTGCGAAGCACCTAAATTCCATTTGGAGGTTTTCCAGGAAGCAGAATTGCTGGTTAACATCAAAGATCACGTTCTCATTCCCGAGCACCAGTTGTTGACAAACGAAGAGAAGAAGACGTTGTGGAGAGGTATACGGTGAAAGATACACAAGTGAGTTTCATTTTATTAGATCTATTTTCTTGAAATACAAGAAACATTGTGAGAATTATCTTCCATAAATATTTTGAAATGACCTTGGTAGATTGGCCTGCGGTGAAGAAGGGCTTTGTAAATAATAAAAATTTTATGAATGAGCTCAAGCCATTATGAGATTTAATATTCTCCGAGTTACATTGGCCATCAAATATAGTCGGGTTAGGCAGTTCGTTAGAATAGTCGCGATGCATGCAACTTGGTTCTGACATTCTTGGATACATATTGAAATGTGTATAGGTTAGTTCCTAATGTGACTTCGTATTTTGTCTTAGCTACCTCGCATGCAGGTTACTGATCCAATTGCTAGATATTATGGAATGAAGCGTGGACAAGTAGTGAAGATCATCCGACCAAGCGAAACTGCGGGAAGATATGTGACATATAGATATGTTGTTTGAGATCAATATGATATGAGCGATTGAAGTCATGGTACTTGCCTATTTGGAAGTGTGGAAGTGGTTTTAGATTTAGGCAATTCTCGTATATGAGCTCAAACTAGGGAATTCTTTTATTTAGAACATTCTTTTATTTAGTAGCTTCTCTGTGTTCCTTCATCTTTATCTTCT

Coding sequence (CDS)

ATGGTGTTGTCAGAGGAAGAAATCACCAGACTCTTTAGAGTCCGCAAAACTGTGTTGCAGATGTTGAGAGACAGAGGCTACATTGTTGGAGACTTTGAGTTTGCTATGTCAAGAGAACAATTTAAAAATCAGTATGGTGAGACCATGAAAAAAGAAGATCTTGTTATCAATAAATCCATGAGAAATAATAGCCCTAACCAGATATATGTTTTCTTTCCTGAAGAGCCGAAGGTTAGTGTCAAGACAGTGCGAACATATGTAAATCGTATGAAATCAGATAATGTTTTTAGAGCAATATTGGTTGTTCAAGATAAATTGTCCTCCTTTGCTCGTTCTTGTATCTGCGAAGCACCTAAATTCCATTTGGAGGTTTTCCAGGAAGCAGAATTGCTGGTTAACATCAAAGATCACGTTCTCATTCCCGAGCACCAGTTGTTGACAAACGAAGAGAAGAAGACGTTGTGGAGAGGTATACGGTGA

Protein sequence

MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSMRNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICEAPKFHLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTLWRGIR

Homology

[Close]

BLAST of PI0007927 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.9e-51
Identity = 97/154 (62.99%), Postives = 130/154 (84.42%), Query Frame = 0


Query: 2   VLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSMR 61
           +L+EEE+ RL+R++KT++QMLRDRGY + D E  M+++QF  ++G+ MK+EDLV  K+ R
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 62  NNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICE-APKF 121
           N++ +Q+Y+FFP+E KV VKT++ Y NRMKS+NVFRAILVVQ  L+ FAR+CI E + KF
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 122 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           HLEVFQEAE+LVNIK+HVL+PEHQ+LT EEKKTL
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTL 154

[Close]

BLAST of PI0007927 vs. ExPASy Swiss-Prot
Match: Q80UW8 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10090 GN=Polr2e PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.7e-29
Identity = 63/152 (41.45%), Postives = 103/152 (67.76%), Query Frame = 0


Query: 5   EEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGE-----TMKKEDLVINKS 64
           EEE  RL+++RKT++Q+  DRGY+V   E   + E+FK Q+G+       ++ DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  MRNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICE-AP 124
             ++  +Q++VFFPEEPKV +KT++ Y  RM+ +N+ RA++VVQ  ++  A+  + + AP
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELLVNIKDHVLIPEHQLLTNEE 151
           K+ LE F + ELL+NI +H L+PEH ++T EE
Sbjct: 124 KYVLEQFLQQELLINITEHELVPEHVVMTKEE 155

[Close]

BLAST of PI0007927 vs. ExPASy Swiss-Prot
Match: B0BNE2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus OX=10116 GN=Polr2e PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.7e-29
Identity = 63/152 (41.45%), Postives = 103/152 (67.76%), Query Frame = 0


Query: 5   EEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGE-----TMKKEDLVINKS 64
           EEE  RL+++RKT++Q+  DRGY+V   E   + E+FK Q+G+       ++ DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  MRNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICE-AP 124
             ++  +Q++VFFPEEPKV +KT++ Y  RM+ +N+ RA++VVQ  ++  A+  + + AP
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELLVNIKDHVLIPEHQLLTNEE 151
           K+ LE F + ELL+NI +H L+PEH ++T EE
Sbjct: 124 KYVLEQFLQQELLINITEHELVPEHVVMTKEE 155

[Close]

BLAST of PI0007927 vs. ExPASy Swiss-Prot
Match: Q5R587 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=9601 GN=POLR2E PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-28
Identity = 62/152 (40.79%), Postives = 103/152 (67.76%), Query Frame = 0


Query: 5   EEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGE-----TMKKEDLVINKS 64
           EEE  RL+++RKT++Q+  DRGY+V   E   + E+F+ Q+G+       ++ DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFRAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  MRNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICE-AP 124
             ++  +Q++VFFPEEPKV +KT++ Y  RM+ +N+ RA++VVQ  ++  A+  + + AP
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELLVNIKDHVLIPEHQLLTNEE 151
           K+ LE F + ELL+NI +H L+PEH ++T EE
Sbjct: 124 KYILEQFLQQELLINITEHELVPEHVVMTKEE 155

[Close]

BLAST of PI0007927 vs. ExPASy Swiss-Prot
Match: P19388 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Homo sapiens OX=9606 GN=POLR2E PE=1 SV=4)

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-28
Identity = 63/152 (41.45%), Postives = 102/152 (67.11%), Query Frame = 0


Query: 5   EEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGE-----TMKKEDLVINKS 64
           EEE  RL+++RKT++Q+  DRGY+V   E   + E+FK Q G+       ++ DL +  +
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQSGDKPSEGRPRRTDLTVLVA 63

Query: 65  MRNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICE-AP 124
             ++  +Q++VFFPEEPKV +KT++ Y  RM+ +N+ RA++VVQ  ++  A+  + + AP
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELLVNIKDHVLIPEHQLLTNEE 151
           K+ LE F + ELL+NI +H L+PEH ++T EE
Sbjct: 124 KYILEQFLQQELLINITEHELVPEHVVMTKEE 155

[Close]

BLAST of PI0007927 vs. ExPASy TrEMBL
Match: A0A1S3C8T2 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498269 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.2e-73
Identity = 144/154 (93.51%), Postives = 151/154 (98.05%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVLSEEEITRLFRVRKTVLQMLRDRG++VGDFE AMSREQFKNQ+GETMKKED+VINKSM
Sbjct: 1   MVLSEEEITRLFRVRKTVLQMLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSM 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICEAPKF 120
           RNNS +QIYVFFPEEPKVSVKTVRTY+NRMKSDNVFRAILVVQ+KLSSFARS ICEAPKF
Sbjct: 61  RNNSSDQIYVFFPEEPKVSVKTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKF 120

Query: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL
Sbjct: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 154

[Close]

BLAST of PI0007927 vs. ExPASy TrEMBL
Match: A0A5A7TIR4 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold302G00120 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.2e-73
Identity = 144/154 (93.51%), Postives = 151/154 (98.05%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVLSEEEITRLFRVRKTVLQMLRDRG++VGDFE AMSREQFKNQ+GETMKKED+VINKSM
Sbjct: 1   MVLSEEEITRLFRVRKTVLQMLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSM 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICEAPKF 120
           RNNS +QIYVFFPEEPKVSVKTVRTY+NRMKSDNVFRAILVVQ+KLSSFARS ICEAPKF
Sbjct: 61  RNNSSDQIYVFFPEEPKVSVKTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKF 120

Query: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL
Sbjct: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 154

[Close]

BLAST of PI0007927 vs. ExPASy TrEMBL
Match: A0A1S4E290 (DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498269 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.2e-73
Identity = 144/154 (93.51%), Postives = 151/154 (98.05%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVLSEEEITRLFRVRKTVLQMLRDRG++VGDFE AMSREQFKNQ+GETMKKED+VINKSM
Sbjct: 1   MVLSEEEITRLFRVRKTVLQMLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSM 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICEAPKF 120
           RNNS +QIYVFFPEEPKVSVKTVRTY+NRMKSDNVFRAILVVQ+KLSSFARS ICEAPKF
Sbjct: 61  RNNSSDQIYVFFPEEPKVSVKTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKF 120

Query: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL
Sbjct: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 154

[Close]

BLAST of PI0007927 vs. ExPASy TrEMBL
Match: A0A0A0LYM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G702030 PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 7.1e-73
Identity = 143/154 (92.86%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVLSEEEIT+LFR RKTVLQMLRDRG++VGDFE AMSREQFKNQYGETMKKEDLVINKSM
Sbjct: 1   MVLSEEEITKLFRARKTVLQMLRDRGFVVGDFELAMSREQFKNQYGETMKKEDLVINKSM 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICEAPKF 120
           RNNS +QIYVFFPEEPKVSVKTVRTY+NRMKSDNVFRAILVVQ+KLSSFARS ICEAPKF
Sbjct: 61  RNNSSDQIYVFFPEEPKVSVKTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKF 120

Query: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           HLEVFQEAELLVNIKDHVLIPEHQLLTNE+KKTL
Sbjct: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEDKKTL 154

[Close]

BLAST of PI0007927 vs. ExPASy TrEMBL
Match: A0A6J1KT45 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111497988 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 4.3e-62
Identity = 124/155 (80.00%), Postives = 142/155 (91.61%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVL+EEEITRLFRVRKTVLQML+DRGY VGDFE  MSREQFKN+YGE+MK+ED+VINK+ 
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICE-APK 120
           RN+S +QIY+FFPEEPKV VKT++TY NRMKS+NVFRAILVVQ  L+ FAR+CI E + K
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 FHLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           FHLEVFQEAELLVNIKDHVL+PEHQLL+NEEKKTL
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTL 155

[Close]

BLAST of PI0007927 vs. NCBI nr
Match: XP_008459046.1 (PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo] >XP_008459047.1 PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo] >XP_008459048.1 PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo] >KAA0043210.1 DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo var. makuwa] >TYK12258.1 DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 284.3 bits (726), Expect = 6.6e-73
Identity = 144/154 (93.51%), Postives = 151/154 (98.05%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVLSEEEITRLFRVRKTVLQMLRDRG++VGDFE AMSREQFKNQ+GETMKKED+VINKSM
Sbjct: 1   MVLSEEEITRLFRVRKTVLQMLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSM 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICEAPKF 120
           RNNS +QIYVFFPEEPKVSVKTVRTY+NRMKSDNVFRAILVVQ+KLSSFARS ICEAPKF
Sbjct: 61  RNNSSDQIYVFFPEEPKVSVKTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKF 120

Query: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL
Sbjct: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 154

[Close]

BLAST of PI0007927 vs. NCBI nr
Match: XP_016902349.1 (PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 [Cucumis melo])

HSP 1 Score: 284.3 bits (726), Expect = 6.6e-73
Identity = 144/154 (93.51%), Postives = 151/154 (98.05%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVLSEEEITRLFRVRKTVLQMLRDRG++VGDFE AMSREQFKNQ+GETMKKED+VINKSM
Sbjct: 1   MVLSEEEITRLFRVRKTVLQMLRDRGFLVGDFELAMSREQFKNQFGETMKKEDIVINKSM 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICEAPKF 120
           RNNS +QIYVFFPEEPKVSVKTVRTY+NRMKSDNVFRAILVVQ+KLSSFARS ICEAPKF
Sbjct: 61  RNNSSDQIYVFFPEEPKVSVKTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKF 120

Query: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL
Sbjct: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 154

[Close]

BLAST of PI0007927 vs. NCBI nr
Match: XP_004145446.1 (DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >KGN66878.1 hypothetical protein Csa_007321 [Cucumis sativus])

HSP 1 Score: 283.1 bits (723), Expect = 1.5e-72
Identity = 143/154 (92.86%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVLSEEEIT+LFR RKTVLQMLRDRG++VGDFE AMSREQFKNQYGETMKKEDLVINKSM
Sbjct: 1   MVLSEEEITKLFRARKTVLQMLRDRGFVVGDFELAMSREQFKNQYGETMKKEDLVINKSM 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICEAPKF 120
           RNNS +QIYVFFPEEPKVSVKTVRTY+NRMKSDNVFRAILVVQ+KLSSFARS ICEAPKF
Sbjct: 61  RNNSSDQIYVFFPEEPKVSVKTVRTYINRMKSDNVFRAILVVQEKLSSFARSSICEAPKF 120

Query: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           HLEVFQEAELLVNIKDHVLIPEHQLLTNE+KKTL
Sbjct: 121 HLEVFQEAELLVNIKDHVLIPEHQLLTNEDKKTL 154

[Close]

BLAST of PI0007927 vs. NCBI nr
Match: KAG6593063.1 (DNA-directed RNA polymerases II and IV subunit 5A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 247.3 bits (630), Expect = 9.0e-62
Identity = 124/155 (80.00%), Postives = 142/155 (91.61%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVL+EEEITRLFRVRKTVLQML+DRGY VGDFE  MSREQFKN+YGE+MK+ED+VINK+ 
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICE-APK 120
           RN+S +QIY+FFPEEPKV VKT++TY NRMKS+NVFRAILVVQ  L+ FAR+CI E + K
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 FHLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           FHLEVFQEAELLVNIKDHVL+PEHQLL+NEEKKTL
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTL 155

[Close]

BLAST of PI0007927 vs. NCBI nr
Match: XP_022959553.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959554.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959555.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_023004795.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004796.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004797.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023513561.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo] >XP_023513563.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 247.3 bits (630), Expect = 9.0e-62
Identity = 124/155 (80.00%), Postives = 142/155 (91.61%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSM 60
           MVL+EEEITRLFRVRKTVLQML+DRGY VGDFE  MSREQFKN+YGE+MK+ED+VINK+ 
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICE-APK 120
           RN+S +QIY+FFPEEPKV VKT++TY NRMKS+NVFRAILVVQ  L+ FAR+CI E + K
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 FHLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           FHLEVFQEAELLVNIKDHVL+PEHQLL+NEEKKTL
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTL 155

[Close]

BLAST of PI0007927 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 203.4 bits (516), Expect = 1.4e-52
Identity = 97/154 (62.99%), Postives = 130/154 (84.42%), Query Frame = 0


Query: 2   VLSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSMR 61
           +L+EEE+ RL+R++KT++QMLRDRGY + D E  M+++QF  ++G+ MK+EDLV  K+ R
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 62  NNSPNQIYVFFPEEPKVSVKTVRTYVNRMKSDNVFRAILVVQDKLSSFARSCICE-APKF 121
           N++ +Q+Y+FFP+E KV VKT++ Y NRMKS+NVFRAILVVQ  L+ FAR+CI E + KF
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 122 HLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           HLEVFQEAE+LVNIK+HVL+PEHQ+LT EEKKTL
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTL 154

[Close]

BLAST of PI0007927 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 114.4 bits (285), Expect = 8.5e-26
Identity = 64/155 (41.29%), Postives = 102/155 (65.81%), Query Frame = 0


Query: 5   EEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETM---KKEDLVINKSMR 64
           ++EITR+F+VR+TVLQMLRDRGY + + +  + RE+F  ++ +TM    KE L ++ +  
Sbjct: 5   DDEITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKG 64

Query: 65  NNSPNQIYVFFPEEPKVSVKTVRTYVN-RMKSDNVFRAILVVQDKLSSFARSCICEAPK- 124
            N  ++IYVF+PE PKV V  ++  V  +M+ D V R I+VV   +++ AR  + E  K 
Sbjct: 65  PNPADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSELNKM 124

Query: 125 FHLEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
             +EVF+EAEL+ NI +H L+ ++ +L ++ KK L
Sbjct: 125 LTIEVFEEAELVTNITEHKLVNKYYVLDDQAKKKL 159

[Close]

BLAST of PI0007927 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 90.5 bits (223), Expect = 1.3e-18
Identity = 58/153 (37.91%), Postives = 87/153 (56.86%), Query Frame = 0


Query: 3   LSEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSMRN 62
           LS EE  R +  R+  LQMLRDRGY V D +  +S   F+  YGE    + L I+   R+
Sbjct: 20  LSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLRISALHRS 79

Query: 63  NSPNQIYVFFPEEPKVSVKTVRTYV-NRMKSDNVFRAILVVQDKLSSFARSCICEAPKFH 122
           +S  ++ + F     V V  +R+ V + +  + +   ILV+Q+ +++ A   I E   F 
Sbjct: 80  DSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAI-ELFSFK 139

Query: 123 LEVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           +E+FQ  +LLVNI  H L P+HQ+L +EEK TL
Sbjct: 140 VEIFQITDLLVNITKHSLKPQHQVLNDEEKTTL 171

[Close]

BLAST of PI0007927 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 83.6 bits (205), Expect = 1.6e-16
Identity = 53/152 (34.87%), Postives = 87/152 (57.24%), Query Frame = 0


Query: 4   SEEEITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSMRNN 63
           S  E  + +  R+T ++MLRDRGY V D +  +S +QF+  YGE    + L I+   R +
Sbjct: 17  SSVECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKHRFD 76

Query: 64  SPNQIYVFFPEEPKVSVKTVRTY-VNRMKSDNVFRAILVVQDKLSSFARSCICEAPKFHL 123
           S  +I V F     V V  +R    + +  +N+   ILV+Q  +++ A   + E   F +
Sbjct: 77  SSKKISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHITNQALKAV-ELFSFKV 136

Query: 124 EVFQEAELLVNIKDHVLIPEHQLLTNEEKKTL 155
           E+F+  +LLVN+  HVL P+HQ+L ++EK++L
Sbjct: 137 ELFEITDLLVNVSKHVLRPKHQVLNDKEKESL 167

[Close]

BLAST of PI0007927 vs. TAIR 10
Match: AT3G54490.1 (RNA polymerase II fifth largest subunit, E )

HSP 1 Score: 82.8 bits (203), Expect = 2.7e-16
Identity = 51/151 (33.77%), Postives = 85/151 (56.29%), Query Frame = 0


Query: 7   EITRLFRVRKTVLQMLRDRGYIVGDFEFAMSREQFKNQYGETMKKEDLVINKSMRNNSPN 66
           E  R +  R T  +MLRDRGY V + E +++  +F++ +GE  + E L I   +R++   
Sbjct: 35  ESKRFYLARTTAFEMLRDRGYEVNEAELSLTLSEFRSVFGEKPELERLRICVPLRSDPKK 94

Query: 67  QIYVFFPEEPKVSVKTVRT-YVNRMKSDNVFRAILVVQDKLSSFARSCICEAPKFHLEVF 126
           +I V F     ++VK+VR  ++    +  +   ILV+Q K++ FA+  +   P F +E F
Sbjct: 95  KILVVFMGTEPITVKSVRALHIQISNNVGLHAMILVLQSKMNHFAQKALTTFP-FTVETF 154

Query: 127 QEAELLVNIKDHVLIPEHQLLTNEEKKTLWR 157
              +LLVNI  H+  P+ ++L  EEK+ L R
Sbjct: 155 PIEDLLVNITKHIQQPKIEILNKEEKEQLLR 184

The following BLAST results are available for this feature:

BLAST of PI0007927 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81098	1.9e-51	62.99	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
Q80UW8	4.7e-29	41.45	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10...	[more]
B0BNE2	4.7e-29	41.45	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus ...	[more]
Q5R587	1.0e-28	40.79	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=96...	[more]
P19388	1.8e-28	41.45	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Homo sapiens OX=96...	[more]

BLAST of PI0007927 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C8T2	3.2e-73	93.51	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A5A7TIR4	3.2e-73	93.51	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 OS=Cucumis mel...	[more]
A0A1S4E290	3.2e-73	93.51	DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 OS=Cucumis mel...	[more]
A0A0A0LYM6	7.1e-73	92.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G702030 PE=3 SV=1	[more]
A0A6J1KT45	4.3e-62	80.00	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36...	[more]

BLAST of PI0007927 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459046.1	6.6e-73	93.51	PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X1 [Cu...	[more]
XP_016902349.1	6.6e-73	93.51	PREDICTED: DNA-directed RNA polymerases II and IV subunit 5A-like isoform X2 [Cu...	[more]
XP_004145446.1	1.5e-72	92.86	DNA-directed RNA polymerases II and IV subunit 5A [Cucumis sativus] >KGN66878.1 ...	[more]
KAG6593063.1	9.0e-62	80.00	DNA-directed RNA polymerases II and IV subunit 5A, partial [Cucurbita argyrosper...	[more]
XP_022959553.1	9.0e-62	80.00	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_...	[more]

BLAST of PI0007927 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22320.1	1.4e-52	62.99	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT5G57980.1	8.5e-26	41.29	RNA polymerase II fifth largest subunit, C	[more]
AT3G57080.1	1.3e-18	37.91	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT2G41340.1	1.6e-16	34.87	RNA polymerase II fifth largest subunit, D	[more]
AT3G54490.1	2.7e-16	33.77	RNA polymerase II fifth largest subunit, E	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	GENE3D	3.40.1340.10		coord: 3..132 e-value: 1.9E-44 score: 153.1
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 5..133
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 132..155 e-value: 1.7E-4 score: 21.4
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 5..90 e-value: 5.2E-30 score: 103.9
None	No IPR available	PANTHER	PTHR10535:SF17	DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKE	coord: 1..155
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 1..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0007927.1	PI0007927.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity